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This paper first presents the O2O teaching strategy for choral conducting and analyzes specific methods and processes for teaching choral conducting using the O2O model. Then, the TFAHP choral conducting teaching evaluation model was constructed, and the judgment matrix was used to calculate the comprehensive degree of fuzzy value of the evaluation criteria and to compare the importance value between the indicators. Lastly, the impact of teaching was evaluated using indicators such as grades, comprehensive performance, and learning emotions. The mean value of the achievement of all knowledge modules is much higher than the mean value before teaching, and the average value of the achievement of each knowledge module is between 0.6-0.85, and the P value of each knowledge module is lower than 0.01, which is an extremely significant difference, indicating that the teaching effect is significant. The innovative development of music education is promoted, and this study greatly impacts choral conducting education.

SUMMARY: The osmotic resistance mechanism has been extensively studied in whole plants or plant tissues. However, little is known about it in embryogenic tissue (ET) which is widely used in plant‐based biotechnological systems. Suberin, a cell wall aliphatic and aromatic heteropolymer, plays a critical role in plant cells against osmosis stress. The suberin regulatory biosynthesis has rarely been studied in gymnosperms. Here, PaMYB11, a subgroup 11 R2R3‐MYB transcription factor, plays a key role in the osmotic resistance of Norway spruce (Picea abies) ETs during cryoprotectant pretreatment. Thus, RNA‐seq, histological, and analytical chemical analyses are performed on the stable transformations of PaMYB11‐OE and PaMYB11‐SRDX in Norway spruce ETs. DAP‐seq, Y1H, and LUC are further combined to explore the PaMYB11 targets. Activation of PaMYB11 is necessary and sufficient for suberin lamellae deposition on Norway spruce embryogenic cell walls, which plays a decisive role in ET survival under osmotic stress. Transcriptome analysis shows that PaMYB11 enhances suberin lamellae monomer synthesis by promoting very long‐chain fatty acid (VLCFA) synthesis. PaPOP, PaADH1, and PaTET8L, the first two (PaADH1 and PaPOP, included) involved in VLCFA synthesis, are proved to be the direct targets of PaMYB11. Our study identified a novel osmotic response directed by PaMYB11 in Norway spruce ET, which provides a new understanding of the resistance mechanism against osmosis in gymnosperms. Significance Statement: In brief, our data suggested that the activation of PaMYB11 enhances SL accumulation by promoting very long‐chain fatty acids synthesis in Picea abies embryogenic tissues. PaADH1, PaPOP and TET8L are direct targets of PaMYB11. However, the correlation between very long‐chain fatty acids synthesis and the expression of PaADH1, PaPOP and TET8L is still unclear. It provides novel insight into the resistance mechanism against osmosis in gymnosperms. [ABSTRACT FROM AUTHOR]