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In this study, the complete mitochondrial genome (mitogenome) of Cotylorhiza tuberculata (Scyphozoa; Rhizostomeae; Cepheidae) was assembled by the next-generation sequencing data. The complete mitogenome spanned 16,590 bp and contained 14 protein-coding genes, two transfer RNA genes, and two ribosomal RNA genes. Total AT% content was 67.7%, comprising A 30.22%, C 16.16%, G 17.05%, and T 36.56%. The gene arrangement exhibited consistency with the known mitogenomes of other jellyfish species. Furthermore, the phylogenetic relationship of C. tuberculata was investigated based on analysis of the 13 common protein-coding genes. Results indicated a close relationship between C. tuberculata and both Cassiopea xamachana and Cassiopea andromeda. These findings provide a valuable reference for advancing understanding of the phylogenetic relationships, taxonomic classification, and phylogeography of jellyfish species. [ABSTRACT FROM AUTHOR]

Abstract In order to investigate the expression levels of nerve growth factor (NGF) and its receptors (TrkA and p75) in prehierarchical follicles and oviducts of hens, five 130-day-old laying hens were examined by immunohistochemistry and RT-PCR analysis. NGF and its receptors were expressed in theca cells and granulosa cells of prehierarchical follicles, and they were also expressed in the epithelial cells of oviducts. The expression of the genes NGF, TrkA and p75 were significantly different in prehierarchical follicles (p

In order to investigate the expression level of nerve growth factor(NGF) and its receptors in the ovary of hens,2 groups (each group contains 5 birds) of 83 and 230 days old laying hens were examined by immunohistochemistry and fluorescence quantitative PCR analyses. The results demonstrated that NGF and its two receptors were not detected in the ovary of 83 days old laying hens,but expressed in thecal cells and granulosa cells of prehierarchical follicles of 230 days old laying hens. Expressions of NGF and its receptors tyrosine kinase A(TrkA) and p75 neurotrophin receptor(p75) in small yellow follicle(SYF) of 230 days old were significantly higher than those in small white follicle( SWF) and large white follicle( LWF). Furthermore, serum levels of estrogen and/ or progesterone were significantly higher in 230 days old than those in 83 days old. Taken together, these results indicated that NGF may play a role in the growth,development and ovulation of hierarchical follicles,and it may also affect the laying rate in different laying periods. [ABSTRACT FROM AUTHOR]

In this study, the complete mitochondrial genome (mitogenome) of Cotylorhiza tuberculata (Scyphozoa; Rhizostomeae; Cepheidae) was assembled by the next-generation sequencing data. The complete mitogenome spanned 16,590 bp and contained 14 protein-coding genes, two transfer RNA genes, and two ribosomal RNA genes. Total AT% content was 67.7%, comprising A 30.22%, C 16.16%, G 17.05%, and T 36.56%. The gene arrangement exhibited consistency with the known mitogenomes of other jellyfish species. Furthermore, the phylogenetic relationship of C. tuberculata was investigated based on analysis of the 13 common protein-coding genes. Results indicated a close relationship between C. tuberculata and both Cassiopea xamachana and Cassiopea andromeda . These findings provide a valuable reference for advancing understanding of the phylogenetic relationships, taxonomic classification, and phylogeography of jellyfish species., Competing Interests: No potential competing interests are reported by the authors., (© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)