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2 results on '"Pseudorabies microbiology"'

1. Detection of pseudorabies virus DNA in the inner ear of intranasally infected BALB/c mice with nucleic acid hybridization in situ

Author

M. Haus, Hans Wolf, I. Bandtlow, and N. Falser

Subjects
Herpesvirus 1, Suid/isolation & purification, Pseudorabies/microbiology, Herpesvirus 4, Human, Immunology, Labyrinth Diseases, 610 Medizin, Pseudorabies, In situ hybridization, Biology, Microbiology, Virus, BALB/c, Ear, Inner/microbiology, Nucleic acid thermodynamics, Mice, Labyrinthitis/microbiology, Virology, DNA, Viral/analysis, medicine, Animals, Inner ear, Labyrinthitis, Frozen section procedure, ddc:610, Mice, Inbred BALB C, Brain, Nucleic Acid Hybridization, Herpesvirus 4, Human/isolation & purification, biology.organism_classification, Herpesvirus 1, Suid, medicine.anatomical_structure, Insect Science, Ear, Inner, DNA, Viral, Brain/microbiology, Nucleic acid, Labyrinth Diseases/microbiology, Research Article
Abstract

Evidence for the pathogenicity of pseudorabies virus for the auditory and vestibular organs of experimentally infected mice is presented. We demonstrate viral genomes in cells of the peripheral sensory organs, the nerve structures, and the affected areas of the brain in single sections from an entire cranium of an adult mouse. The data were obtained by an in situ hybridization technique adapted for use with fixed, plastic-embedded materials. In contrast to conventional methods which use frozen sections, we were able to analyze cartilaginous and bony materials with high resolution.

Published
1986

2. Demonstration of pseudorabies virus DNA in the mouse inner ear by an in situ nucleic acid hybridization technique in plastic embedded bony material

Author

I. Bandtlow, N. Falser, M. Haus, and Hans Wolf

Subjects
In situ, Pseudorabies/microbiology, Pathology, medicine.medical_specialty, Histology, 610 Medizin, Pseudorabies, In situ hybridization, Virus, Pathology and Forensic Medicine, Ear, Inner/microbiology, Mice, Nucleic acid thermodynamics, chemistry.chemical_compound, Neural Pathways, Labyrinthitis/pathology, DNA, Viral/analysis, medicine, Animals, Methylmethacrylates, Brain/pathology, Inner ear, Labyrinthitis, Mice, Inbred BALB C, Neural Pathways/pathology, ddc:610, biology, DNA–DNA hybridization, Histological Techniques, Brain, Herpesvirus 1, Suid/genetics, Nucleic Acid Hybridization, Cochlea/pathology, biology.organism_classification, Herpesvirus 1, Suid, Cochlea, medicine.anatomical_structure, chemistry, Ear, Inner, DNA, Viral, Autoradiography, sense organs, DNA
Abstract

This investigation is concerned with the possibility of identifying viral DNA using the in situ DNA hybridization method in methylmethacrylate-embedded material. As an experimental model we chose viral labyrinthitis produced by intranasal infection of the mouse with pseudorabies virus. Fixation and embedding methods specially adapted to this procedure and bony histology preparation technique (specimens by grinding or micromilling) made it possible to identify viral DNA directly morphologically and virologically in the inner ear. Quantitative microphotometric analyses of trans-sagittal sections of the entire skull after in situ DNA hybridization are presented and discussed here as an explicit method of investigating the path of distribution of viral DNA in the brain and the inner ear.

Published
1986

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