Search

Background Quercus salicina (Blume) leaves are traditionally used as folk medicine in some Asian countries. The aim of this study was to evaluate the effects of ball milling for different periods (0, 6, 12, 18, and 24 h) on the physicochemical properties of superfine Quercus salicina (Blume) leaf (QSL) powders. Results The particle sizes, water-holding capacity, angle of repose, and redness of the superfine QSL powder decreased with increasing ball-milling times, whereas the water solubility index, bulk density, tapped density, brightness, and yellowness were found to increase. Significantly higher (P > 0.05) total phenolic and flavonoid contents, and antioxidant activities, were observed for the superfine QSL powders obtained after 24 h ball-milling time. A total of 12 phenolic compounds in free and cell-wall-bound forms were quantified in the superfine QSL powder. Free phenolics such as protocatechuic acid, caffeic acid, rutin, and p-coumaric acid were increased and all cell-wall-bound phenolics were decreased with increasing ball-milling times. The antioxidant activity of the free phenolics increased with increasing ball-milling times, and the cell-wall-bound forms decreased. Conclusion Superfine grinding by ball milling for 24 h can thus be used to produce superfine QSL powder with higher free phenolic metabolite content and antioxidant activity, and improved water solubility index, color, bulk, and tapped densities. This study will be useful for the food / nutraceutical / pharmaceutical industries in the manufacturing of active food ingredients or value-added products using QSL powders. © 2020 Society of Chemical Industry.

The effects of two different freezing temperatures (− 20 °C and − 80 °C) on the astringency trait of persimmon fruits during 15 to 60 days of storage were investigated. The levels of soluble and insoluble tannins, proanthocyanidins and other physicochemical characteristics were evaluated. Storage at − 20 °C and − 80 °C temperatures up to 60 days has been found to be an effective method to remove astringency of persimmon fruits. Proanthocyanidin concentration was negligible at both temperatures during storage. Total soluble solid contents were decreased as 3.34 from 4.59 (mg/g DW) whereas, insoluble tannin contents were increased as 20.30 from 16.45 (mg/g DW) by freezing temperatures treatment during storage. Comparatively, higher soluble tannin content 11.68 (mg/g DW) and lower insoluble tannin content 10.02 (mg/g DW) was observed in control (day 0). Therefore, the astringency of persimmon fruits incubated at − 20 °C and − 80 °C was markedly reduced and after 15 up to 60 days of storage, the astringent taste virtually disappeared. The proanthocyanidin contents were decreased as 0.02 from 0.52 (mg/g DW) at − 20 °C storage and 0.17 from 0.47 (mg/g DW) at − 80 °C storage, in comparison with the control 2.65 (mg/g DW). The moreover, along with the removal of astringency, other physicochemical parameters including color, pH, moisture content, total soluble solid, and sensory attributes were also conserved on freezing at both the temperatures. These findings suggest that freezing temperature treatments aid the removal of astringency from persimmon fruits which could be used in different food preparations or as supplements. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13197-020-04818-3) contains supplementary material, which is available to authorized users.

Carotenoids are potent antioxidants, but they are highly unstable and susceptible during processing and storage. Encapsulation technologies protect against degradation and are capable of releasing individual or combination of bioactive substances during processing as well as development of various functional food products. Moreover, encapsulating agents can be used to increase the stability of carotenoids and form a barrier between the core and wall materials. Suitable encapsulating agents, temperature, and drying methods are the most important factors for the encapsulation process. In this report, we reviewed the current status of encapsulation of carotenoids from different fruits, vegetables, spices, seaweeds, microorganisms, and synthetic sources using various types of encapsulating agents through spray drying and freeze drying. We also focused on the degradation kinetics and various factors that affect the stability and bioavailability of encapsulated carotenoids during their processing and storage.

Three muscadine grape genotypes (Muscadinia rotundifolia (Michx.) Small) were evaluated for their metabolite profiling and antioxidant activities at different berry developmental stages. A total of 329 metabolites were identified using UPLC-TOF-MS analysis (Ultimate 3000LC combined with Q Exactive MS and screened with ESI-MS) in muscadine genotypes throughout different developmental stages. Untargeted metabolomics study revealed the dominant chemical groups as amino acids, organic acids, sugars, and phenolics. Principal component analysis indicated that developmental stages rather than genotypes could explain the variations among the metabolic profiles of muscadine berries. For instance, catechin, epicatechin-3-gallate, and gallic acid were more accumulated in ripening seeds (RIP-S). However, tartaric acid and malonic acid were more abundant during the fruit-set (FS) stage, and malic acid was more abundant in the veraison (V) stage. The variable importance in the projection (VIP &gt, 0.5) in partial least-squares–discriminant analysis described 27 biomarker compounds, representing the muscadine berry metabolome profiles. A heatmap of Pearson’s correlation analysis between the 27 biomarker compounds and antioxidant activities was able to identify nine antioxidant determinants, among them, gallic acid, 4-acetamidobutanoic acid, trehalose, catechine, and epicatechin-3-gallate displayed the highest correlations with different types of antioxidant activities. For instance, DPPH and FRAP conferred a similar antioxidant activity pattern and were highly correlated with gallic acid and 4-acetamidobutanoic acid. This comprehensive study of the metabolomics and antioxidant activities of muscadine berries at different developmental stages is of great reference value for the plant, food, pharmaceutical, and nutraceutical sectors.

Eight blueberry cultivars at three developmental stages were investigated for metabolite profiling, antioxidant, and anticancer activities. Cultivars- and developmental stages-variations were determined in total phenolic, flavonoid, DPPH, and FRAP antioxidant assays. The anticancer capacity was equal against A549, HepG2, and Caco-2 cancer cells, whereas the inhibition rate was dose-, incubation period-, cultivar-, and developmental stages-dependent. The untargeted metabolite profiling by UPLC-TOF-MS analysis of two contrast cultivars, 'Vernon' and 'Star', throughout the developmental stages revealed 328 metabolites; the majority of them were amino acids, organic acids, and flavonoids. The multivariate statistical analysis identified five metabolites, including quinic acid, methyl succinic acid, chlorogenic acid, oxoadipic acid, and malic acid, with positively higher correlations with all anticancer activities. This comprehensive database of blueberry metabolites along with anticancer activities could be targeted as natural anticancer potentials. This study would be of great value for food, nutraceutical, and pharmaceutical industries as well as plant biotechnologists.

Green tea contains bioactive compounds, such as polyphenols, responsible for its health-promoting effects, including antiobesity and antidiabetic effects. We previously reported that ultra-sonication extraction (UE) could efficiently increase the extraction yield of green tea compounds. In the present study, we found that the extract obtained using UE contained higher phenolic and flavonoid contents than that obtained using the conventional method. We therefore considered the extract as a bioactive metabolite-rich functional green tea extract (BMF-GTE), and tested its glucose-lowering effect by generating an adipocyte cell line stably expressing 7myc-GLUT4-GFP. We found that BMF-GTE treatment increased GLUT4 translocation to the plasma membrane. Moreover, BMF-GTE administration attenuated weight gain in mice fed a high-fat diet (HFD). Importantly, HFD-induced glucose tolerance was ameliorated in the mice receiving BMF-GTE. Therefore, we conclude that BMF-GTE worked against obesity and diabetes, at least partially, by enhancing GLUT4 translocation in adipocytes. PRACTICAL APPLICATIONS: As green tea is one of the most consumed beverages worldwide, its health effects have been widely tested. In our previous studies, we found that ultra-sonication extraction (UE) has the potential to increase the aqueous extraction yield of green tea compounds compared to conventional extraction techniques. In this study, we examined the biological effect of bioactive metabolite-rich functional green tea extract (BMF-GTE) obtained using UE; we observed that administering BMF-GTE lowered the body weight and increased insulin sensitivity in mice fed a high-fat diet, potentially by facilitating the membrane translocation of GLUT4 in adipocytes. Therefore, this study suggests that the extract obtained with UE had antiobesity and antidiabetic properties, indicative of a potential application of UE in maximizing the beneficial effects of green tea on human health.

Research on oxidative stress–related pathogenicities is gaining immense attention. Oxidative stress is induced by reactive species that are byproducts of biological metabolic reactions or arise due to environmental factors. Oxidative stress is crucial in cellular dysfunctions, wherein cellular components such as lipids, proteins, and nucleic acids are oxidized, thereby leading to several disorders including metabolic syndrome, cardiovascular disorders, neurodegenerative disorders, and cancer. Natural dietary antioxidants have been reported to reduce oxidative stress and to prevent associated diseases. Tea is considered as a predominant source of natural antioxidants. The major antioxidants present in tea include phenolic metabolites, although nonphenolic metabolites also possess prospective activity against oxidative stress. In this chapter, the role of oxidative stress–induced pathophysiology in several disorders is summarized, and the antioxidant potentialities of dietary antioxidants considering the phenolic and nonphenolic metabolites in tea have been described.

The aim of this study was to compare methods for efficient extraction of green tea metabolites. Aqueous green tea leaf powder extracts were prepared by ultra-sonication extraction (UE), agitation extraction (AE), and the conventional extraction method (control) at different temperatures (60, 70, and 80 °C) and times (5–30 min). Compared to the control, both UE and AE techniques significantly increased the yields of polyphenols (three-fold), catechins (two-fold), and flavonoids (two-fold), and resulted in higher antioxidant activity. For both techniques, highest extraction efficiency of bioactive metabolites occurred at 80 °C for 20 min. Caffeine, total free amino acid, vitamin C, and total soluble solid contents were also significantly increased using either technique compared to the control. Thus, AE and UE have similar extraction efficiencies and could be used to prepare antioxidant-rich green tea extract or green tea extract powder.

Volatile compounds and non-phenolic metabolites (amino acids, organic acids, and sugars) of aqueous green tea extracts obtained by ultrasonic extraction (UE), agitation extraction (AE), hot water extraction (HWE), and conventional extraction (CE) were determined using SPME-GC-MS and HPLC, respectively. Significantly higher (P 0.05) yields of volatiles and non-phenolic metabolites were obtained via UE and AE than via HWE and CE. UE, AE, HWE, and CE released 212, 201, 103, and 65 volatiles, respectively. Sum total of amino acid and organic acid in extracts was 54.57, 54.35, 27.11, and 12.67 (mg/100 g), and 5.96, 6.19, 3.81, and 1.68 (mg/100 g) for UE, AE, HWE, and CE, respectively. Volatiles except nitrogen-containing compounds had higher positive correlations with l-theanine, sucrose, malic acid, and catechins yields. Findings of the current study suggest that an efficient extraction technique may significantly increase volatile and non-phenolic metabolite yields in aqueous green tea extract.

In this study, phenolic metabolites in their free, soluble esterified, and soluble glycosylated forms in green tea infusions and cell wall-bound forms in solid residues obtained by ultrasound treatment, agitation treatment, and normal infusion preparation method were investigated. In total, thirty-three phenolic compounds were characterized including twenty-two flavonoids and eleven phenolic acids using UPLC-DAD-QToF/MS analysis. The flavonoids were sub-characterized as flavanols and flavonols, and phenolic acids were sub-characterized as hydroxybenzoic acid, hydroxybenzoylquinic acid, hydroxycinnamic acid, and hydroxycinnamoylquinic acid. As flavanols, EGCG was dominant in their free form and was significantly higher (p

To characterize the p-coumaric acid (pCA)-induced phenolic metabolites in relation to the disease resistance against Xanthomonas campestris pv. campestris (Xcc.), the responses of soluble- and cell wall-bound flavonoid and hydroxycinnamic acids compounds to the pretreatment of pCA or the inhibitor of the 4-coumarate-CoA ligase, 3,4-(methylenedioxy) cinnamic acid (MDCA), following Xcc inoculation were assessed, and the resulting data were interpreted with regard to susceptibility to Xcc in Chinese cabbage (Brassica rapa var. pekinensis). At 12 days post-inoculation (DPI) with Xcc, disease symptom development could be distinguished by necrotic lesions, and characterized by an enhanced lipid peroxidation. Overall, pCA acts as a positive stimulus for an accumulation of hydroxycinnamic acids and flavonoids, while MDCA acts as a negative regulator. Pretreatment with pCA resulted in an accumulation of specific hydroxycinnamic acids, pCA, ferulic acid (FA), and sinapic acid (SiA) in both soluble and cell wall-bound forms in Xcc-inoculated leaves, while MDCA pretreatment decreased accumulation in a dose-dependent manner. Two flavonoid compounds, epigallocatechin (EGC) and epigallocatechin gallate (EGCG), showed a similar response to pCA and MDCA pretreatments. These results indicate that a lower disease symptom development in pCA-pretreated leaves was associated with a higher accumulation of hydroxycinnamic acids and flavonoids, and vice-versa in MDCA- and non-pretreated (control) leaves.

Background: Herpes simplex viruses (HSV) are communal an infection has been established an alarming problem around the world. Objective: The objectives of this study are to scrutinize age and sex stratified prevalence in HSV seroprevalence in Dhaka the most populated metropolitan the capital of Bangladesh and under a timeline January, 2014 to June, 2014. Patients and Methods: This cross-sectional privately representative survey was carried out at the department of biochemistry, Primeasia University, Dhaka, Bangladesh. Importance was given on statistical inferences for national seroprevalence of herpes simplex virus estimates. Analysis was done using standard procedure. Result: Infected individuals were more likely to be female than male (50.0% vs 25.6%; P

Context: Medicinal practices of the tribes of Bangladesh remain largely un-documented. Aims: The aim of the present study was to conduct an ethnomedicinal survey and documentation among the Nag clan of the Rai Ghatual tribe of Bangladesh. Settings and Design: The survey was carried out among the Nag clan of the Rai Ghatual tribal community of Moulvibazar district. The clan, according to them, is the only Nag clan of the Rai Ghatual tribe in Bangladesh. The clan has three tribal healers, still continuing their traditional medicinal practices. Materials and Methods: Interviews of the healers were carried out with the help of a semi-structured questionnaire and the guided field-walk method. Results: The Nag clan healers were observed to use 28 different plant species distributed into 22 families for treatment of ailments such as fever, loss of appetite, male infertility, dysentery, lower abdominal pain during menstruation, jaundice, stomachache, burning sensations during urination, bodily pain and weak health. Conclusions: This is the first reported study of the traditional medicinal practices of Nag clan healers. Several of the plants can be validated in their uses on the basis of existing scientific literature. The medicinal plants used by the Nag healers warrant further scientific studies, for the plants are readily available and can form alternative medicinal sources instead of costlier biomedical drugs.

The Mandais are a little known tribe of Bangladesh inhabiting the north central regions, particularly Tangail district of Bangladesh. Their population has been estimated to be less than 10,000 people. Although the tribe has for the most part assimilated with the mainstream Bengali-speaking population, they to some extent still retain their original tribal customs, including their traditional medicinal practices. Since this practice is also on the verge of disappearance, the objective of the present study was to conduct an ethnomedicinal survey among Mandai tribal practitioners to document their use of medicinal plants for treatment of various ailments. Four traditional practitioners were found in the exclusive Mandai-inhabited village of Chokchokia in Tangail district. Information was collected from the practitioners with the help of a semi-structured questionnaire and guided field-walk method. It was observed that the four traditional practitioners used a total of 31 plants distributed into 23 families for treatment. The various ailments treated included diabetes, low semen density, jaundice, gastrointestinal tract disorders (stomach ache, indigestion, dysentery, and diarrhea), leucorrhea, pain (rheumatic pain, joint pain), skin disorders, respiratory tract disorders (coughs, mucus, and allergy), debility, fever, and helminthiasis. From the number of plants used (seven), it appeared that gastrointestinal tract disorders formed the most common ailment among the Mandai community, possibly brought about by the low income status of the people coupled with unhygienic conditions of living.