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2 results on '"Protein sulfhydril"'

1. Determination of protein thiolation index (PTI) as a biomarker of oxidative stress in human serum.

Author

Giustarini, Daniela, Galvagni, Federico, Colombo, Graziano, Dalle-Donne, Isabella, Milzani, Aldo, Aloisi, Anna Maria, and Rossi, Ranieri

Subjects
*THIOLS, *BLOOD proteins, *OXIDATIVE stress, *BLOOD coagulation, *BLOOD serum analysis
Abstract

We have introduced protein thiolation index (PTI), i.e. the molar ratio of the sum of all low molecular mass thiols bound to plasma proteins to protein free cysteinyl residues, as a sensitive biomarker of oxidative stress. According to the original procedure its determination requires a rapid separation of plasma and a specific treatment of samples to stabilize thiols. Here we demonstrate that samples can be collected without use of any anticoagulant to prevent blood clotting and without any stabilization of thiols too. This simplification of the determination of PTI makes its analysis more feasible also in routine clinical laboratories. [ABSTRACT FROM AUTHOR]

Published
2017
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2. Determination of protein thiolation index (PTI) as a biomarker of oxidative stress in human serum

Author

Aldo Milzani, Ranieri Rossi, Isabella Dalle-Donne, Daniela Giustarini, Federico Galvagni, Anna Maria Aloisi, and Graziano Colombo

Subjects
Serum, 0301 basic medicine, Adult, Male, Biomarkers, Oxidative stress, Protein sulfhydril, S-thiolation, Aged, Blood Chemical Analysis, Blood Coagulation, Blood Proteins, Female, Humans, Middle Aged, Smoking, Sulfhydryl Compounds, Young Adult, Oxidative Stress, Spectrophotometry, Biophysics, Biochemistry, Molecular Biology, Cell Biology, 030204 cardiovascular system & hematology, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, Molar ratio, medicine, Chromatography, Blood clotting, Molecular mass, Chemistry, Blood proteins, 030104 developmental biology, Protein free, Biomarker (medicine)
Abstract

We have introduced protein thiolation index (PTI), i.e. the molar ratio of the sum of all low molecular mass thiols bound to plasma proteins to protein free cysteinyl residues, as a sensitive biomarker of oxidative stress. According to the original procedure its determination requires a rapid separation of plasma and a specific treatment of samples to stabilize thiols. Here we demonstrate that samples can be collected without use of any anticoagulant to prevent blood clotting and without any stabilization of thiols too. This simplification of the determination of PTI makes its analysis more feasible also in routine clinical laboratories.

Published
2017

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