1. Efficiency of erythropoietin's signal peptide for HIV(MN)-1 gp 120 expression.
- Author
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Herrera AM, Musacchio A, Fernández JR, and Duarte CA
- Subjects
- Amino Acid Sequence, Animals, COS Cells, Erythropoietin biosynthesis, Gene Expression, Genetic Vectors, HIV Envelope Protein gp120 biosynthesis, HIV-1 metabolism, Humans, Molecular Sequence Data, Protein Sorting Signals biosynthesis, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Tissue Plasminogen Activator biosynthesis, Tissue Plasminogen Activator genetics, Transfection, Erythropoietin genetics, HIV Envelope Protein gp120 genetics, HIV-1 genetics, Protein Sorting Signals genetics
- Abstract
The HIV-1 gp120 gene with natural signal sequence expressed in eukaryotic expression systems showed extremely low levels of synthesis and secretion. Several expression systems have been used to improve the secretion levels of gp 120. In mammalian cells, the efficient expression of gp120 fused to t-PA signal peptide has been previously reported. Here, the effects of t-PA and EPO signal peptides were compared as secretion sequences for expression of gp120 in COS-7 cells. The EPO's signal peptide is used for the first time as leader sequence for secretion of foreign proteins. Our results indicated that higher amounts of secreted gp 120 were obtained when vectors containing EPO signal peptide were used., (Copyright 2000 Academic Press.)
- Published
- 2000
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