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154 results on '"Prokopowicz, M."'

1. Carbon Nanomaterials Application as a Counter Electrode for Dye-Sensitized Solar Cells

Author

Dobrzański L. A., Prokopiuk vel Prokopowicz M., Drygała A., Wierzbicka A., Lukaszkowicz K., and Szindler M.

Subjects
dye-sensitized solar cell, carbon nanomaterials, counter electrode, nanocrystalline photovoltaic cells, Mining engineering. Metallurgy, TN1-997, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

The paper presents the results of the structure investigation of a counter electrode in dye-sensitized solar cells using the carbon nanomaterials. Solar cells were fabricated on the glass with transparent conductive oxide TCO (10Ω/sq). Nanocrystalline titania based photoanode was prepared by spreading TiO2 paste onto TCO glass and subsequently annealed at 450°C for at least 30 min to convert anatase phase and make an interparticle network. After then the nanostructured titania films was immersed into an ethanolic solution of the ruthenium-based dye. As a counter electrodes of dye-sensitized solar cells composite films of carbon nanomaterials and polystyrene sulfonate doped poly (3,4-ethylenedioxythiophene) PEDOT-PSS (Sigma-Aldrich) were deposited onto TCO substrates. Because carbon nanoelements and titanium oxide consist of nano-metric structural units to determine the properties of the cells and their parameters several surface sensitive techniques and methods, i.e. Raman spectroscopy, Scanning Electron Microscopy (SEM), High-Resolution Transmission Electron Microscopy (HRTEM), and electric properties of conductive layers were used.

Published
2017
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2. Nanocrystalline TiO2 Powder Prepared by Sol-Gel Method for Dye-Sensitized Solar Cells

Author

Dobrzański L. A., Szindler M. M., Szindler M., Lukaszkowicz K., Drygała A., and Prokopiuk vel Prokopowicz M.

Subjects
sol-gel, nanopowder, titanium dioxide, dye-sensitized solar cell, Mining engineering. Metallurgy, TN1-997, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

In this study titanium dioxide nanopowder has been manufactured and examined. Nanocrystalline TiO2 powder has been obtained by hydrolysis and peptization of a solution of titanium isopropoxide and isopropanol. Subsequently, produced powder has been subjected to structural analysis by using a transmission electron microscope, X-ray diffractometer, and Raman spectrometer. For comparison purposes, a commercially available titanium dioxide powder (i.e. titanium white) was also used. Thin layers have been made from this powder and further have been examined by using a UV/VIS spectrometer. Completed research shows the nanocrystalline structure of obtained layers and their good properties such as absorbance at the range of wavelength equal 200 - 1000 nm.

Published
2016
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3. Carbon Nanotubes Counter Electrode for Dye-Sensitized Solar Cells Application

Author

Drygała A., Dobrzański L. A., Szindler M., Prokopiuk vel Prokopowicz M., Pawlyta M., and Lukaszkowicz K.

Subjects
dye-sensitized solar cells, counter electrode, carbon nanotubes, TEM., Mining engineering. Metallurgy, TN1-997, Materials of engineering and construction. Mechanics of materials, TA401-492
Abstract

The influence of the carbon nanotubes counter electrode deposited on the FTO glass substrates on the structure and optoelectrical properties of dye-sensitized solar cells counter electrode (CE) was analysed. Carbon materials have been applied in DSSC s in order to produce low-cost solar cells with reasonable efficiency. Platinum is a preferred material for the counter electrode because of its high conductivity and catalytic activity. However, the costs of manufacturing of the platinum counter electrode limit its use to large-scale applications in solar cells. This paper presents the results of examining the structure and properties of the studied layers, defining optical properties of conductive layers and electrical properties of dye-sensitized solar cells manufactured with the use of carbon nanotubes.

Published
2016
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10. P981Lvot area measurement using gated ct data reclassifies aortic stenosis severity as graded by echocardiographyP982Paradoxical low-flow low-gradient aortic stenosis: an intermediate state between moderate and severe aortic stenosis?P983Can rheumatic significant mitral stenosis be a cause of paradoxical low gradient, low flow, in patients with severe aortic stenosis? an echocardiographic and outcome studyP984Clinical and hemodynamic comparison of isolated versus combined aortic and mitral stenosisP985Echocardiographic end-diastolic velocity in the proximal descending aorta should be interpreted with caution when the ascending aorta is dilated: insights from cardiovascular magnetic resonanceP987Prevalence of atrial mitral regurgitation in patients with severe mitral regurgitationP988Role of 2D/3D echocardiography in the risk stratification of endocardial lead-related tricuspid regurgitation: a single-centre study among?241 patientsP989When TEE is needed in patients with staphylococcus aureus bacteremia for the assessment of risk profile of infective endocarditis?P990Appropriateness criteria to echocardiograms for suspected infective endocarditis: experience of a tertiary referral centerP991Independent predictors of outcome in infective endocarditisP992The role of transesophageal cardiography in clinical course and prognosis of complicated infective endocarditis in critically ill patients: our 15 years experienceP993Left bundle branch block atypical pattern as a prognostic determinant in patients taken to TAVIP994Efficacy of long-term ivabradine therapy in severe systolic chronic heart failure patients with and without type 2 diabetes mellitusP995Relations between left ventricular reverse remodeling and serum markers of extracellular matrix fibrosis in dilated cardiomyopathyP996The healthy left ventricle accommodates an increasing vortex formation time for volume transfer in diastolic filling :Implications for heart failureP997Evolutionary changes of pulmonary artery pressure after left ventricular assist device implantP998Functional correlates and prognostic value of coronary flow velocity reserve by vasodilator stress echocardiography in hypertrophic cardiomyopathyP999Quantification of myocardial performance in patients with non-obstructive versus latent-obstructive hypertrophic cardiomyopathyP1000Lifelong arrhythmic risk stratification in arrhythmogenic right ventricular cardiomyopathy: distribution of events and impact of periodical reassessmentP1001Impact of fibrosis visualized by CMR in vectorcardiogram recordings of patients with suspected arrhythmogenic cardiomyopathyP1002Determinants of the beneficial effect of aldosterone antagonism on exercise capacity in heart failure with reduced ejection fractionP1003Myocardial strain values in patients with acute myocarditis and preserved ejection fraction. A magnetic resonance feature tracking studyP1004Detection of subclinical left ventricular dysfunction by speckle tracking echocardiography in patients with myocarditis without prominent wall motion abnormalitiesP1005Aborted sudden cardiac death patients aged <50 years show only mild alterations on cardiac magnetic resonance imagingP1006Relationships between subepicardial and subendocardial longitudinal strain with late gadolinium enhancement in uncomplicated hypertensive patients

Author

Moderato, L., primary, Di Nora, C., primary, Soufiani, A., primary, Bech-Hanssen, O., primary, Johnson, K., primary, Hamadanchi, A., primary, Longobardo, L., primary, Casadei, F., primary, Ilhao Moreira, R., primary, Ahmad, F., primary, Parra Laca, B., primary, Tumasyan, LR., primary, Wisniowska-Smialek, S., primary, King, GJ., primary, Di Ruocco, MV., primary, Ciampi, Q., primary, Wang, J., primary, Cappelletto, C., primary, Igual Munoz, B., primary, Kosmala, W., primary, Almeida, A M G, primary, Kostakou, P., primary, Zaremba, T., primary, Contaldi, C., primary, Palumbo, A., additional, Coli, S., additional, Orlandini, D., additional, Russo, G., additional, Gaibazzi, N., additional, Poli, S., additional, Vriz, O., additional, Zito, C., additional, Carerj, S., additional, Pavan, D., additional, Antonini-Canterin, F., additional, Ougnou, H., additional, Elhattab, FZ., additional, Bendagha, N., additional, Tribak, M., additional, Lahchab, FZ., additional, Mahfoudi, L., additional, Fadel, B., additional, Moughil, S., additional, Mohty, D., additional, El Hattab, FZ., additional, Aboyans, V., additional, Polte, CL., additional, Svensson, F., additional, Johnsson, ÅA, additional, Lagerstrand, KM., additional, Gao, SA., additional, Abdel-Rahman, ST., additional, Dobson, L., additional, Gatenby, K., additional, Kidambi, A., additional, Schlosshan, D., additional, Moradi, B., additional, Asadi, Y., additional, Poerner, TC., additional, Doenst, T., additional, Schulze, PC., additional, Goebel, B., additional, Trio, O., additional, Cusma'-Piccione, M., additional, Di Bella, G., additional, Khandheria, BK., additional, De Chiara, B., additional, Spano', F., additional, Bokor, D., additional, Quattrocchi, S., additional, Varrenti, M., additional, Vigano', E., additional, Rinaldi, I., additional, Giannattasio, C., additional, Moreo, A., additional, Coutinho Cruz, M., additional, Moura Branco, L., additional, Galrinho, A., additional, Coutinho Miranda, L., additional, Viveiros Monteiro, A., additional, Aguiar Rosa, S., additional, Rodrigues, I., additional, Fragata, J., additional, Cruz Ferreira, R., additional, Cox, S., additional, Chan, ST., additional, Abdul Rahman, E., additional, Zainal Abidin, I., additional, Sadiq, MA., additional, Islas, F., additional, De Agustin, A., additional, Nombela, L., additional, Marcos-Alberca, P., additional, Macaya, C., additional, Perez De Isla, L., additional, Olmos, C., additional, Adamyan, KG., additional, Rubis, P., additional, Khachatryan, L., additional, Karabinowska, A., additional, Faltyn, P., additional, Dziewiecka, E., additional, Biernacka-Fialkowska, B., additional, Hlawaty, M., additional, Kostkiewicz, M., additional, Podolec, P., additional, Coen, K., additional, Serseniuc, M., additional, Nolan, PG., additional, Clarke, JG., additional, Temporelli, PL., additional, Pistono, M., additional, Imparato, A., additional, Eleuteri, E., additional, Giannuzzi, P., additional, Cortigiani, L., additional, Tesic, M., additional, Beleslin, B., additional, Rigo, F., additional, Djorkievic-Dikic, A., additional, Picano, E., additional, Zuo, L., additional, Ta, SJ., additional, Kang, N., additional, Zhou, MY., additional, Guo, RQ., additional, Liu, L., additional, Stolfo, D., additional, De Luca, A., additional, Pinamonti, B., additional, Barbati, G., additional, Gobbo, G., additional, Pivetta, A., additional, Brun, F., additional, Merlo, M., additional, Sinagra, G., additional, Sanz, JS., additional, Molina-Aguilar, PMA, additional, Castells Ramon, FCR, additional, Giner Blasco, JGB, additional, Santiago Jimenez, SG., additional, Maceira-Gonzalez, AMG, additional, Monserrat Reina, AMR, additional, Tuset Sanchez, LTS, additional, Zorio-Grima, EZG, additional, Sacharczuk, W., additional, Kotwica, T., additional, Dankowski, R., additional, Przewlocka-Kosmala, M., additional, Kobusiak-Prokopowicz, M., additional, Mysiak, A., additional, Szyszka, A., additional, Menezes, MN., additional, Lima Silva, G., additional, Guimaraes, T., additional, Francisco, AR., additional, Placido, R., additional, David, C., additional, Pinto, FJ., additional, Kouris, N., additional, Trifou, E., additional, Kostopoulos, V., additional, Hatzigiannis, P., additional, Olympios, CD., additional, Broendberg, AK., additional, Jensen, HK., additional, Kim, WY., additional, Imbriaco, M., additional, Alcidi, G., additional, Ponsiglione, A., additional, Santoro, C., additional, Puglia, M., additional, Barbuto, L., additional, Cuocolo, A., additional, Trimarco, B., additional, and Galderisi, M., additional

Published
2016
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14. Nanocrystalline TiO2 Powder Prepared by Sol-Gel Method for Dye-Sensitized Solar Cells.

Author

Dobrzański, L. A., Szindler, M. M., Szindler, M., Lukaszkowicz, K., Drygała, A., and Prokopiuk vel Prokopowicz, M.

Subjects
DYE-sensitized solar cells, TITANIUM dioxide, NANOCRYSTALS, SOL-gel processes, METAL powders
Abstract

In this study titanium dioxide nanopowder has been manufactured and examined. Nanocrystalline TiO2 powder has been obtained by hydrolysis and peptization of a solution of titanium isopropoxide and isopropanol. Subsequently, produced powder has been subjected to structural analysis by using a transmission electron microscope, X-ray diffractometer, and Raman spectrometer. For comparison purposes, a commercially available titanium dioxide powder (i.e. titanium white) was also used. Thin layers have been made from this powder and further have been examined by using a UV/VIS spectrometer. Completed research shows the nanocrystalline structure of obtained layers and their good properties such as absorbance at the range of wavelength equal 200 - 1000 nm. [ABSTRACT FROM AUTHOR]

Published
2016
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15. Abstracts

Author

Barthelemy, O., primary, Silvain, J., additional, Brieger, D., additional, Bellemain-Appaix, A., additional, Cayla, G., additional, Beygui, F., additional, Lancar, R., additional, Collet, J. P., additional, Mercadier, A., additional, Montalescot, G., additional, Cha, K. S., additional, Nam, Y. H., additional, Kim, J. H., additional, Park, S. Y., additional, Park, T. H., additional, Kim, M. H., additional, Kim, Y. D., additional, Lee, H. C., additional, Ahn, M. S., additional, Hong, T. J., additional, Blanco, R., additional, Blanco, F., additional, Szarfer, J., additional, Garcia Escudero, A., additional, Gigena, G., additional, Gagliardi, J., additional, Rodriguez, A., additional, Sarmiento, R., additional, Affatatto, S., additional, Riccitelli, M., additional, Petris, A., additional, Datcu, M. D., additional, Pop, C., additional, Radoi, M., additional, Arsenescu-Georgescu, C., additional, Petrescu, I., additional, Petrescu, L., additional, Serban, L., additional, Nechita, E., additional, Tatu-Chitoiu, G., additional, Dorobantu, M., additional, Benedek, I., additional, Craiu, E., additional, Sinescu, C., additional, Ionescu, D. D., additional, Ginghina, C., additional, Minescu, B., additional, Izzo, A., additional, Mantovani, P., additional, Tomasi, L., additional, Dall'oglio, L., additional, Bonatti, S., additional, Rosiello, R., additional, Romano, M., additional, Agostini, F., additional, Zanini, R., additional, Zhao, Z. Y., additional, Wu, Y. J., additional, Li, J. J., additional, Yany, Y. J., additional, Qian, H. Y., additional, Tang, Y. D., additional, Timoteo, A. T., additional, Toste, A., additional, Lousinha, A., additional, Ramos, R., additional, Oliveira, J. A., additional, Ferreira, M. L., additional, Ferreira, R. C., additional, Cabades, C., additional, Diez Gil, J. L., additional, Aguar, P., additional, Sanmiguel, D., additional, Lopez-March, A., additional, Marmol, R., additional, Guerra, L., additional, Girbes, V., additional, Ferrando, J., additional, Rincon De Arellano, A., additional, Patricio, L., additional, Blondal, M., additional, Ainla, T., additional, Marandi, T., additional, Eha, J., additional, Oliveira, M. M., additional, Silva, M. N., additional, Cunha, P. S., additional, Feliciano, J., additional, Silva, S., additional, Kanovsky, J., additional, Kala, P., additional, Parenica, J., additional, Poloczek, M., additional, Prymusova, K., additional, Kubkova, L., additional, Spinar, J., additional, Olinic, D., additional, Homorodean, C., additional, Ober, M., additional, Olinic, M., additional, Andrioaia, C., additional, Condac, A., additional, Masmoudi, M., additional, Berdaoui, B., additional, Labidi, S., additional, Tapia Ballesteros, C., additional, Hernandez Luis, C., additional, Sandin, M. G., additional, Vegas, J. M., additional, Andion, R., additional, Martinez, N., additional, Gonzalez, I. A., additional, Alvarado, M., additional, Amat, I. J., additional, San Roman, J. A., additional, Garcia Gonzalez, M. J., additional, Arroyo Ucar, E., additional, Hernandez Garcia, C., additional, Dorta Martin, M., additional, Marrero Rodriguez, F., additional, Dragu, R., additional, Kapeliovich, M., additional, Hammerman, H., additional, Silva, D., additional, Cortez-Dias, N., additional, Jorge, C., additional, Silva Marques, J., additional, Carilho Ferreira, P., additional, Robalo Martins, S., additional, Almeida Ribeiro, M., additional, Calisto, C., additional, Fiuza, M., additional, Lopes, M. G., additional, Milicevic, P., additional, Panic, M., additional, Stankovic, I., additional, Milicevic, D., additional, Kalezic, T., additional, Kafedzic, S., additional, Ilic, I., additional, Cerovic, M., additional, Putnikovic, B., additional, Neskovic, A., additional, Rott, D., additional, Leibowitz, D., additional, Monhart, Z., additional, Reissigova, J., additional, Grunfeldova, H., additional, Jansky, P., additional, Valente, B., additional, Villanueva Benito, I., additional, Solla, I., additional, Paredes, E., additional, Diaz Castro, O., additional, Calvo, F., additional, Baz, J. A., additional, Iniguez, A., additional, Aleksova, A., additional, Gerloni, R., additional, Belfiore, R., additional, Carriere, C., additional, Barbati, G., additional, Fabris, E., additional, Possa, F., additional, Nait, D., additional, Milo, M., additional, Sinagra, G., additional, Marques, N., additional, Mimoso, J., additional, Gomes, V., additional, Agra Bermejo, R. M., additional, Emad Abu Assi, E. A. A., additional, Sergio Raposeiras Roubin, S. R. R., additional, Pilar Cabanas Grandio, P. C. G., additional, Carlos Pena Gil, C. P. G., additional, Jose Maria Garcia Acuna, J. M. G. A., additional, Jose Ramon Gonzalez Juanatey, J. R. G. J., additional, Daly, M. J., additional, Scott, P., additional, Owens, C. G., additional, Tomlin, A., additional, Smith, B., additional, Adgey, A. A. J., additional, Alvarez-Contreras, L. R., additional, Juarez, U., additional, Altamirano, A., additional, Arias, A., additional, Alvarez-San Gabriel, A., additional, Gonzalez-Pacheco, H., additional, Martinez-Sanchez, C., additional, Rahnavardi, M., additional, Keshtkar-Jahromi, M., additional, Vakili, H., additional, Gholamin, S., additional, Razavi, S. M., additional, Gilis-Januszewski, T., additional, Mellwig, K.- P., additional, Wiemer, M., additional, Gilis-Januszewski, J., additional, Peterschroeder, A., additional, Koerfer, J., additional, Horstkotte, D., additional, Vrsalovic, M., additional, Getaldic, B., additional, Vrkic, N., additional, Pintaric, H., additional, Khan, S., additional, Wasan, B., additional, Moretti, L., additional, Grossi, P., additional, Silenzi, S., additional, Testa, M., additional, Candelori, L., additional, Clementi, L. N., additional, Forlini, M., additional, Lando, L., additional, Pezzuoli, M. L., additional, Corradetti, P., additional, Leurent, G., additional, Pennec, P. Y., additional, Filippi, E., additional, Moquet, B., additional, Hacot, J. P., additional, Druelles, P., additional, Rialan, A., additional, Rouault, G., additional, Coudert, I., additional, Le Breton, H., additional, Gevaert, S., additional, Tromp, F., additional, Vandecasteele, E., additional, De Somer, F., additional, Van Belleghem, Y., additional, Bouchez, S., additional, Martens, F., additional, Herck, I., additional, De Pauw, M., additional, Ludka, O., additional, Sepsi, M., additional, Miklik, R., additional, Dusek, L., additional, Tomcikova, D., additional, Garcia-Acuna, J. M., additional, Aguiar-Souto, P., additional, Raposeiras Roubin, S., additional, Agra-Bermejo, R., additional, Jacquet, M., additional, Abu-Assi, E., additional, Gonzalez-Juanatey, J. R., additional, Ibatov, A., additional, Labrova, R., additional, Karlik, R., additional, Lokaj, P., additional, She, Q., additional, Deng, S. B., additional, Huang, S. H., additional, Gu, L. J., additional, Rong, J. I. A. N., additional, Wu, Z. K., additional, Li, Y., additional, Zhang, J., additional, Parascan, L., additional, Campanile, A., additional, Spinelli, L., additional, Santulli, G., additional, Ciccarelli, M., additional, De Gennaro, S., additional, Assante Di Panzillo, E., additional, Trimarco, B., additional, Iaccarino, G., additional, Bobescu, E., additional, Datcu, G., additional, Dobreanu, D., additional, Doka, B., additional, Charniot, J.- C., additional, Cosson, C., additional, Albertini, J. P., additional, Bittar, R., additional, Giral, P., additional, Cherfils, C., additional, Guillerm, E., additional, Bonnefont-Rousselot, D., additional, Rusali, A., additional, Cojocaru, L., additional, Parepa, I., additional, Koizumi, T., additional, Iida, S., additional, Sato, J., additional, Kikutani, T., additional, Muramatsu, T., additional, Nishimura, S., additional, Komiyama, N., additional, Lee, W. P., additional, Ong, B. B., additional, Haralambos, K., additional, Townsend, D., additional, Rees, J. A. E., additional, Williams, E. J., additional, Halcox, J. P., additional, Mcdowell, I., additional, Damjanovic, M., additional, Koracevic, G., additional, Djordjevic-Radojkovic, D., additional, Pavlovic, M., additional, Krstic, N., additional, Ciric-Zdravkovic, S., additional, Stojkovic, A., additional, Perisic, Z., additional, Apostolovic, S., additional, Faustino, A., additional, Seca, L., additional, Barra, S., additional, Caetano, F., additional, Providencia, R., additional, Silva, J., additional, Gomes, P., additional, Costa, G., additional, Costa, M., additional, Leitao-Marques, A., additional, Volkova, A. L., additional, Arutyunov, G. P., additional, Bylova, N. A., additional, Dayter, I. I., additional, Jao, Y. T. F. N., additional, Fang, C. C., additional, Chen, Y., additional, Yu, C. L., additional, Wang, S. P., additional, Valencia, J., additional, Perez-Berbel, P., additional, Ruiz-Nodar, J. M., additional, Pineda, J., additional, Bordes, P., additional, Quintanilla, M., additional, Mainar, V., additional, Sogorb, F., additional, Santos, N., additional, Serrao, M., additional, Cafe, H., additional, Silva, B., additional, Oliveira, R., additional, Caires, G., additional, Drumond, A., additional, Araujo, J., additional, Providencia, R. A., additional, Gomes, P. L., additional, Pais, J. R., additional, Mota, P., additional, Leitao-Marques, A. M., additional, Farhan, S., additional, Jarai, R., additional, Tentzeris, I., additional, Vogel, B., additional, Freynhofer, M. K., additional, Wojta, J., additional, Huber, K., additional, Poli, M., additional, Trambaiolo, P., additional, Corsi, F., additional, De Luca, M., additional, Mustilli, M., additional, Lukic, V., additional, Simonetti, M., additional, Ferraiuolo, G., additional, Lettino, M., additional, Casella, G., additional, Conte, M. R., additional, De Luca, L., additional, Geraci, G., additional, Ceravolo, R., additional, Pani, A., additional, Fradella, G., additional, Schratter, A., additional, Thiele, H., additional, Klemm, T., additional, Demmin, K., additional, Lehmann, D., additional, Mende, M., additional, Schuler, G., additional, Pittl, U., additional, Chernova, A., additional, Nikulina, S. U., additional, Naruke, T., additional, Inomata, T., additional, Yanagisawa, T., additional, Maekawa, E., additional, Mizutani, T., additional, Shinagawa, H., additional, Nishii, M., additional, Takeuchi, I., additional, Takehana, H., additional, Izumi, T., additional, Paulo, C., additional, Mascarenhas, J., additional, Patacho, M., additional, Pimenta, J., additional, Bettencourt, P., additional, Nardai, S., additional, Szabo, G. Y., additional, Berta, B., additional, Edes, I., additional, Merkely, B., additional, Delgado Silva, J., additional, Baptista, R., additional, Faria, R., additional, Trigo, J., additional, Gago, P., additional, Gheorghe, G., additional, Nanea, I. T., additional, Cristea, A., additional, Almarichi, S., additional, Martins, H., additional, Saraiva, F., additional, Jorge, E., additional, Mendes, P. L., additional, Monteiro, P., additional, Costa, S., additional, Franco, F., additional, Providencia, L. A., additional, Nanea, T., additional, Gheorghe, G. S., additional, Visan, S., additional, Paun, N., additional, Gaber, R., additional, Delewi, R., additional, Nijveldt, R., additional, De Bruin, H. A., additional, Hirsch, A., additional, Van Der Laan, A., additional, Bouma, B. J., additional, Tijssen, J. P. G., additional, Van Rossum, A. C., additional, Zijlstra, F., additional, Piek, J. J., additional, Rus, H., additional, Donea, M., additional, Ciurea, C., additional, Ifteni, G., additional, Casolo, G., additional, Chioccioli, M., additional, Magnacca, M., additional, Del Meglio, J., additional, Comella, A., additional, Baratto, M., additional, Lera, J., additional, Salvadori, L., additional, Tessa, C., additional, Vignali, C., additional, Keca, Z., additional, Momcilov Popin, T., additional, Panic, G., additional, White, R., additional, Mateen, F., additional, Weaver, A., additional, Agmon, Y., additional, Okisheva, E., additional, Tsaregorodtsev, D., additional, Sulimov, V., additional, Amat Santos, I. J., additional, Hernandez, C., additional, Tapia, C., additional, Campo, A., additional, Fredman, D., additional, Svensson, L., additional, Rosenqvist, M., additional, Tadel-Kocjancic, S., additional, Radsel, P., additional, Knafelj, R., additional, Gorjup, V., additional, Noc, M., additional, Zima, E., additional, Jenei, Z. S., additional, Kovacs, E., additional, Osztheimer, I., additional, Molnar, L., additional, Horvath, A., additional, Becker, D., additional, Geller, L., additional, Maggi, R., additional, Furukawa, T., additional, Viscardi, V., additional, Brignole, M., additional, Leal, S. R. N., additional, Dores, H., additional, Rosario, I., additional, Monge, J., additional, Carvalho, M. J., additional, Arroja, I., additional, Leitao, A., additional, Fonseca, C., additional, Aleixo, A., additional, Silva, A., additional, Keuleers, S., additional, Herijgers, P., additional, Herregods, M. C., additional, Budts, W., additional, Dubois, C., additional, Meuris, B., additional, Verhamme, P., additional, Flameng, W., additional, Van De Werf, F., additional, Adriaenssens, T., additional, Badran, H., additional, Elnoamany, M., additional, Lolah, T., additional, Olariu, C., additional, Macarie, C., additional, Mollik, M. A. H., additional, Hassan, A. I., additional, Paul, T. K., additional, Haque, M. Z., additional, Jahan, R., additional, Rahmatullah, M., additional, Khatun, M. A., additional, Rahman, M. T., additional, Chowdhury, M. H., additional, Bustamante Munguira, J., additional, Tamayo, E., additional, Garcia-Cuenca, I., additional, Bustamante, E., additional, Gualis, J., additional, Gomez-Martinez, M. L., additional, Florez, S., additional, Gomez-Herreras, J. I., additional, Ramirez Rodriguez, R., additional, Ramirez Rodriguez, A. M., additional, Garcia-Bello, M. A., additional, Hernadez Ortega, E., additional, Caballero Dorta, E., additional, Garcia Quintana, A., additional, Piro Mastraccio, V., additional, Medina Fernandez Aceytuno, A., additional, Assanelli, E., additional, De Metrio, M., additional, Rubino, M., additional, Lauri, G., additional, Cabiati, A., additional, Campodonico, J., additional, Grazi, M., additional, Moltrasio, M., additional, Marana, I., additional, Marenzi, G., additional, Lovlien, M., additional, Schei, B., additional, Picon-Heras, R., additional, Acebal, C., additional, Garcia Rubira, J. C., additional, Vivas Balcones, D., additional, Nunez-Gil, I., additional, Ruiz-Mateos, B., additional, Ibanez, B., additional, Fernandez-Ortiz, A., additional, Vintila, V. D., additional, Enescu, O. A., additional, Stoicescu, C. 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J., additional, Gomez Salvador, I., additional, San Roman Calvar, J. A., additional, Mamarasulov, T. M., additional, Todorovic, L., additional, Cherneva, Z. C. H., additional, Denchev, S. D., additional, Heltai, K., additional, Boytsov, A., additional, Nikulina, N. N., additional, Zanna, D., additional, Marangelli, V., additional, Caiati, C., additional, Picon Heras, R., additional, Loureiro, M. J., additional, Urazovskaya, I., additional, Vinogradova, D., additional, Vasilieva, E., additional, Shpektor, A., additional, Conti, E., additional, Musumeci, M. B., additional, Lauri, F. M., additional, Dito, E., additional, De Giusti, M., additional, Lallo, A., additional, Fusco, D., additional, Davoli, M., additional, Volpe, M., additional, Autore, C., additional, Gamra, H., additional, Dridi, Z., additional, Hassine, M., additional, Addad, F., additional, Gherissi, I., additional, Reda, A., additional, Mahjoub, M., additional, Bouraoui, S., additional, Abdennadher, M., additional, Betbout, F., additional, Mota, P. M. F. P., additional, Silva, J. D., additional, Jankovic Tomasevic, R., additional, Djordjevic, V., additional, Djordjevic Radojkovic, D., additional, Scafa Udriste, A., additional, Fruntelata, A., additional, Gainoiu, E., additional, Bogdan, S., additional, Zamfir, D., additional, Teodorescu, C., additional, Guran, M., additional, Constantinescu, D., additional, Konopka, A., additional, Banaszewski, M., additional, Wojtkowska, I., additional, Stepinska, J., additional, Vidergold, J. V., additional, Osipova, I. V., additional, Tavrovskaya, T. V., additional, Galkina, J. V., additional, Timofeev, A. V., additional, Vorobyov, R. I., additional, Vorobyova, E. N., additional, Matos, L., additional, Carvalho, A. C. C., additional, Oliveira, W., additional, Cintra, F., additional, Poyares, D., additional, Andersen, M., additional, Martins, R., additional, Tufik, S., additional, Ostadal, P., additional, Brada, J., additional, Horakova, S., additional, Mlcek, M., additional, Hrachovina, V., additional, Kittnar, O., additional, Gorudko, I. V., additional, Buko, I. V., additional, Cherenkevich, S. N., additional, Polonetsky, L. Z., additional, Plotkin, V. Y., additional, Timoshina, M. A., additional, Azanchevskaya, S. V., additional, Chromov-Borisov, N. N., additional, Vorlat, A., additional, Snoep, L., additional, Claeys, M. J., additional, Vrints, C. J., additional, Palazzuoli, A., additional, Caputo, M., additional, Quatrini, I., additional, Calabro, A., additional, Antonelli, G., additional, Campagna, M. S., additional, Franci, B., additional, Nuti, R., additional, Maisel, A., additional, Negrini, M., additional, Minora, T., additional, Marino, P., additional, Seregni, R., additional, Tavlueva, E., additional, Barbarash, O., additional, Barbarash, L., additional, Janota, T., additional, Kudlicka, J., additional, Malik, K., additional, Wichterle, D., additional, Hradec, J., additional, Body, R., additional, Carley, S. D., additional, Mcdowell, G., additional, Nuttall, M., additional, Wibberley, C., additional, France, M., additional, Cruickshank, J. K., additional, Mackway-Jones, K., additional, Leon, M., additional, Cozma, C., additional, Mitu, F., additional, Almeida, D. R., additional, Dias, C. B., additional, Burazor, I., additional, Burazor, M., additional, Krstic, M., additional, Lazovic, M., additional, Vukmanovic, M., additional, Djordjevic, J., additional, Radovanovic, Z., additional, Ilic, D., additional, Bosnjakovic, P., additional, Ferreira, A. C., additional, Mateus, P. S., additional, Fontes, P., additional, Teixeira, T., additional, Conte, G., additional, Menozzi, A., additional, Solinas, E., additional, Bolognesi, M. G., additional, Tadonio, I., additional, Mantovani, F., additional, Cattabiani, A., additional, Vignali, L., additional, Ardissino, D., additional, Tautu, O., additional, Alexandrescu, A., additional, Niculescu, R., additional, Jankovic, R., additional, Bozinovic, N., additional, Santos, C., additional, Costa, F., additional, Cardoso, G., additional, Correia, I., additional, Fountoulaki, K., additional, Kastellanos, S., additional, Voltirakis, E., additional, Kokotos, A., additional, Michalakeas, C., additional, Kontsas, K., additional, Hasioti, K., additional, Iliodromitis, E. T., additional, Sandin Fuentes, M. G., additional, Zatarain Nicolas, E., additional, Martinez Uruena, N., additional, Alvarado Montes De Oca, M., additional, Dytrych, V., additional, Kovarnik, T., additional, Smid, O., additional, Kral, A., additional, Aroutunov, A. G., additional, Intwala, S., additional, Jegere, I., additional, Shaalan, H. S. H., additional, Pagava, Z., additional, Agladze, R., additional, Shakarishvili, R., additional, Sharashidze, N., additional, Gujejiani, L., additional, Saatashvili, G., additional, Katova, T. Z., additional, Kostova, V., additional, Simova, Y., additional, Vukotic, S., additional, Rafajlovski, S., additional, Romanovic, R., additional, Antonijevic, N., additional, Gligic, B., additional, Hutyra, M., additional, Skala, T., additional, Horak, D., additional, Vindis, D., additional, Taborsky, M., additional, Contine, A., additional, Del Pinto, M., additional, Angeli, F., additional, Verdecchia, P., additional, Borgognoni, F., additional, Grikstaite, E., additional, Pantano, P., additional, Ambrosio, G., additional, Cavallini, C., additional, Bonanad, C., additional, Sanchis, J., additional, Bodi, V., additional, Nunez, J., additional, Bosch, X., additional, Heras, M., additional, Pellicer, M., additional, Llacer, A., additional, Adao, L., additional, Oliveira, M., additional, Goncalves, H., additional, Primo, J., additional, Gama, V., additional, Lombardi, C., additional, Metra, M., additional, Bugatti, S., additional, Pasotti, E., additional, Quinzani, F., additional, Adamo, M., additional, Villa, C., additional, Rovetta, R., additional, Manerba, A., additional, Mariani, M., additional, Dushpanova, A., additional, Baroni, M., additional, Cerone, E., additional, Nardelli, A., additional, Gianetti, J., additional, Berti, S., additional, Feliciano, F., additional, Soares, R., additional, Santos, S., additional, Kruger, A., additional, Vondrakova, D., additional, Herget, J., additional, Navarro, C., additional, Cromie, N. A., additional, Adgey, J. A. A., additional, Caeiro Pereira, D., additional, Braga, P., additional, Fontes Carvalho, R., additional, Rodrigues, A., additional, Goncalves, M., additional, Simoes, L., additional, and Borisov, K. V., additional

Published
2010
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17. AQM-MD simulation approach to the analysis of FRET processes in (bio)molecular systems. A case study: complexes of E. coli purine nucleoside phosphorylase and its mutants with formycin A.

Author

Sobieraj, M., Krzyśko, K. A., Jarmuła, A., Kalinowski, M. W., Lesyng, B., Prokopowicz, M., Cieśla, J., Gojdź, A., and Kierdaszuk, B.

Abstract

Abstract Predicting FRET pathways in proteins using computer simulation techniques is very important for reliable interpretation of experimental data. A novel and relatively simple methodology has been developed and applied to purine nucleoside phosphorylase (PNP) complexed with a fluorescent ligand — formycin A (FA). FRET occurs between an excited Tyr residue (D*) and FA (A). This study aims to interpret experimental data that, among others, suggests the absence of FRET for the PNPF159A mutant in complex with FA, based on novel theoretical methodology. MD simulations for the protein molecule containing D*, and complexed with A, are carried out. Interactions of D* with its molecular environment are accounted by including changes of the ESP charges in S1, compared to S0, and computed at the SCF-CI level. FRET probability WF depends on the inverse six-power of the D*-A distance, Rda. The orientational factor 02<4 between D* and A is computed and included in the analysis. Finally WF is time-averaged over the MD trajectories resulting in its mean value. The red-shift of the tyrosinate anion emission and thus lack of spectral overlap integral and thermal energy dissipation are the reasons for the FRET absence in the studied mutants at pH 7 and above. The presence of the tyrosinate anion results in a competitive energy dissipation channel and red-shifted emission, thus in consequence in the absence of FRET. These studies also indicate an important role of the phenyl ring of Phe159 for FRET in the wild-type PNP, which does not exist in the Ala159 mutant, and for the effective association of PNP with FA. In a more general context, our observations point out very interesting and biologically important properties of the tyrosine residue in its excited state, which may undergo spontaneous deprotonation in the biomolecular systems, resulting further in unexpected physical and/or biological phenomena. Until now, this observation has not been widely discussed in the literature. [ABSTRACT FROM AUTHOR]

Published
2015
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23. In Vitro Measurement of Conformational Stability of Fibrinogen Adsorbed on Siloxane

Author

Prokopowicz, M., L-tukasiak, J., Banecki, B., and Przyjazny, A.

Abstract

The paper describes the effect of 10-, 50-, 100-, 200-, 300-, 400-, and 500-fold excess of low-molecular weight, linear, hexamethyldisiloxane (L2) and cyclic hexamethylcyclotrisiloxane (D3) with respect to fibrinogen (Fbg) on the conformational stability of Fbg during a 60-h incubation at 37 °C. This study used SPE/HPLC/UV and fluorescence of a tryptophan located in the domain of Fbg and fluorescence of 1,1‘-bis(4-anilino)naphthalene-5,5‘-disulfonic acid (ANS) spectroscopy. The fact that the decrease in fluorescence intensity of siloxane-treated Fbg was accompanied by red shift in the maximum wavelength indicated that denaturation of Fbg had taken place. The differences (the decrease in peak height and the shift in retention time) in chromatograms between control Fbg and siloxane-treated Fbg indicated the adsorption process of Fbg on the surface of siloxanes. Incubating hexamethyldisiloxane with Fbg at L2-to-Fbg ratios >300 for 20 h yielded white and mould-like precipitates of Fbg. The same phenomenon demonstrating massive denaturation and aggregation was observed for a greater than 500-fold excess of D3 with respect to Fbg after 20 h of incubation.

Published
2005

31. 14. Ventricular Fibrillation & Out-of-Hospital Cardiac Arrest.

Author

Mysiak, A., Glabisz, W., Kubler, A., Salomon, P., Pielka, S., Solski, L., and Kobusiak-Prokopowicz, M.

Abstract

Introduction Chaos theory is particularly attractive to those, who are studying ventricular fibrillation, because of the way it describes complex phenomena. Methods The study group consisted of 15 rabbits, which were anesthetized (midazolam dose 0,75 mg/kg/h) and then ventricular fibrillation (VF) was induced: in 5 cases by pacing transesophageal electrode (12 V, 50 Hz); in 5 by epinephrine (1mg/kg) and in 5 by calcium intravenously (0,001/kg). After 5 minutes of cardiac arrest defibrillation shock was delivered. If defibrillation was unsuccessful the cardiopulmonary resuscitation was started. ECG data were analyzed using a numerical technique, based on discrete signal wavelet packet analysis of determining chaotic states of a signal. Results Transition from a non-chaotic state to a chaotic state manifested itself in the magnitude and distribution of system response wavelet expansion coefficients. No features connected with chaotic processes were observed during sinus rhythm and ventricular fibrillation. Features connected with chaotic processes were associated only with ventricular tachycardia. Conclusions 1. The standard EKG registration does not allow the analysis of developed chaotic process in myocardium during ventricular fibrillation. 2. Chaotic characteristic could be found in ventricular tachycardia. [ABSTRACT FROM PUBLISHER]

Published
2005
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32. A new approach to generation of standard gas mixtures used in the calibration of gas analyzers

Author

Namiesnik, J., Prokopowicz, M., and Konieczka, P.

Subjects
MEASUREMENT, SILICA gel, HAZARDOUS wastes, CALIBRATION
Abstract

A new method of generation of gaseous standard mixtures has been developed in which the measured component is generated by thermal decomposition of a compound chemically bonded to the surface of silica gel.The method enables preparation of standard mixture immediately before the calibration step. Consequently, it can be applied for the generation of standard mixtures containing volatile, malodorous, unstable and toxic compounds. Examples are thiols, isothiocyanates, amines. [ABSTRACT FROM AUTHOR]

Published
1999

33. Evaluation of the Safety and Potential Benefits of Beetroot-Based Dietary Supplements According to Their Elemental Composition.

Author

Brzezińska-Rojek J, Rutkowska M, Ośko J, Konieczka P, Prokopowicz M, and Grembecka M

Subjects
Humans, Trace Elements analysis, Spectrophotometry, Atomic, Dietary Supplements analysis, Beta vulgaris chemistry
Abstract

The study aimed to assess the health value and safety associated with the consumption of the chosen 37 beetroot-based dietary supplements (DSs). An optimized and validated analytical procedure, using a method called microwave plasma atomic emission spectrometry (MP-AES), was developed to determine the profiles of 19 elements (Na, K, Fe, Ca, Pt, Zn, Cd, Cu, V, Co, Ni, Pb, Mo, Mg, Al, Mn, Sr, Cr, Ba) in the DSs. The products were assessed for compliance with the recommended daily doses for the chosen elements, and any deviations were identified. Results showed that powders constituted a richer source of elements than capsules and tablets. The exception was iron-enriched products, which provided the highest dose of Fe (3.75 to 25 mg/daily dose). Safety assessment was evaluated in 3 steps, including (1) the determination of levels of Al, Ba, Cd, and Pb; (2) comparison of their content with the permissible contamination limits; and (3) comparison of the weekly or monthly intake of Al and Cd with the provisional tolerable weekly (PTWI) or monthly (PTMI) intake, respectively. The content of Ba was evaluated because of the oral reference dose (RfD). Five products were significantly contaminated with Cd (4-134% of PTMI Cd), two with Al (11-12%), and three with Ba (1.085-1.331 µg/d.d.). Lead was not detected above the LOQ (0.035 mg/kg). Factor analysis was employed to differentiate between the pharmaceutical forms (capsules, tablets, and powders) and determine the origin of the powder contained in the capsules. These results highlight the importance of implementing more stringent control measures and regulatory changes in the DSs market to ensure consumer safety., (© 2023. The Author(s).)

Published
2024
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34. An unusual long-term follow-up of a patient with a left ventricular pseudoaneurysm after myocardial infarction.

Author

Zachwyc J, Kobusiak-Prokopowicz M, Guziński M, and Kuliczkowski W

Subjects
Humans, Follow-Up Studies, Heart Ventricles diagnostic imaging, Aneurysm, False complications, Aneurysm, False diagnostic imaging, Myocardial Infarction complications, Heart Rupture, Post-Infarction, Heart Aneurysm complications, Heart Aneurysm diagnostic imaging
Published
2024
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35. Biocompatible antibiotic-loaded mesoporous silica/bioglass/collagen-based scaffolds as bone drug delivery systems.

Author

Skwira A, Szewczyk A, Barros J, Laranjeira M, Monteiro FJ, Sądej R, and Prokopowicz M

Subjects
Chick Embryo, Animals, Tissue Scaffolds chemistry, Silicon Dioxide chemistry, Drug Delivery Systems, Collagen chemistry, Bone and Bones, Ciprofloxacin pharmacology, Porosity, Biocompatible Materials chemistry, Bone Regeneration, Anti-Bacterial Agents pharmacology, Osteomyelitis drug therapy
Abstract

Local delivery of antibiotics has gained increasing interest in the treatment of osteomyelitis due to its effectiveness and safety. Since the regeneration of bone tissue at the site of infection is as important as bacterial eradication, implantable drug delivery systems should not only release the drugs in a proper manner but also exert the osseointegration capability. Herein, we present an implantable drug delivery system in a scaffold form with a unique set of features for local treatment of osteomyelitis. For the first time, collagen type I, ciprofloxacin-loaded mesoporous silica, and bioglass were combined to obtain scaffolds using the molding method. Drug-loaded mesoporous silica was blended with polydimethylsiloxane to prolong the drug release, whereas bioglass served as a remineralization agent. Collagen-silica scaffolds were evaluated in terms of physicochemical properties, drug release rate, mineralization potential, osteoblast response in vitro, antimicrobial activity, and biological properties using an in vivo preclinical model - chick embryo chorioallantoic membrane (CAM). The desirable multifunctionality of the proposed collagen-silica scaffolds was confirmed. They released the ciprofloxacin for 80 days, prevented biofilm development, and induced hydroxyapatite formation. Moreover, the resulting macroporous structure of the scaffolds promoted osteoblast attachment, infiltration, and proliferation. Collagen-silica scaffolds were also biocompatible and effectively integrated with CAM., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Published
2023
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36. Antioxidant Capacity, Nitrite and Nitrate Content in Beetroot-Based Dietary Supplements.

Author

Brzezińska-Rojek J, Sagatovych S, Malinowska P, Gadaj K, Prokopowicz M, and Grembecka M

Abstract

Due to the high content of bioactive substances, beetroot and its preserves might be a valuable constituent of a diet. Research into the antioxidant capacity and content of nitrate (III) and (V) in beetroot-based dietary supplements (DSs) worldwide is limited. The Folin-Ciocalteu method, CUPRAC, DPPH, and Griess methods were used to determine total antioxidant capacity, total phenolic content, nitrites, and nitrates content in fifty DSs and twenty beetroot samples. Moreover, the safety of products was evaluated because of the concentration of nitrites, nitrates, and the correctness of labelling. The research showed that a serving of fresh beetroot provides significantly more antioxidants, nitrites, and nitrates than most daily portions of DSs. Product P9 provided the highest dose of nitrates (169 mg/daily dose). However, in most cases, the consumption of DSs would be associated with a low health value. The acceptable daily intake was not exceeded in the cases of nitrites (0.0015-0.55%) and nitrates (0.056-48%), assuming that the supplementation followed the manufacturer's recommendation. According to European and Polish regulations, 64% of the products tested did not meet all the requirements for labelling food packaging. The findings point to the need for tighter regulation of DSs, as their consumption might be dangerous.

Published
2023
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37. Bioglass obtained via one-pot synthesis as osseointegrative drug delivery system.

Author

Skwira A, Szewczyk A, Sądej R, and Prokopowicz M

Subjects
Powders metabolism, Ceramics, Osteoblasts, Drug Delivery Systems, Glass, Calcium metabolism, Biocompatible Materials metabolism
Abstract

Osseointegration is a fundamental process during which implantable biomaterial integrates with host bone tissue. The surgical procedure of biomaterial implantation is highly associated with the risk of bacterial infection. Thus, the research continues for biodegradable bone void fillers which are able to stimulate the bone tissue regeneration and locally deliver the antibacterial agent. Herein, we obtained bifunctional bioglass (BG) using novel, preoptimized, rapid one-pot synthesis. Following the ISO Standards, the influence of the obtained BG on osteoblast-mediated phenomena, such as osteoconduction and osteoinduction was assessed and compared to two commercial materials: bioactive glass powder 45S and bioactive glass powder 85S. Direct-contact tests revealed osteoblast adhesion to BG particles; whereas, tests on extracts confirmed high viability of cells incubated with BG extract. Analyses of gene expression, alkaline phosphatase activity, and calcium phosphates deposition confirmed the stimulation of early and late stages of osteoblast differentiation and mineralization. Additionally, an extended evaluation of intracellular calcium fluctuations revealed a possible correlation between osteoblast calcium uptake and extracellular matrix mineralization. Moreover, proposed bioglass exhibited satisfactory doxycycline adsorption capacity and release profile. The obtained results confirmed the bifunctionality of the proposed BG and indicated its potential as osseointegrative bone drug delivery system., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Published
2023
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38. Cardiovascular, Pulmonary, and Neuropsychiatric Short- and Long-Term Complications of COVID-19.

Author

Kobusiak-Prokopowicz M, Fułek K, Fułek M, Kaaz K, Mysiak A, Kurpas D, Beszłej JA, Brzecka A, and Leszek J

Subjects
Humans, Pandemics, SARS-CoV-2, Post-Acute COVID-19 Syndrome, Lung, COVID-19 complications
Abstract

Beginning with the various strategies of the SARS-CoV-2 virus to invade our bodies and manifest infection, and ending with the recent long COVID, we are witnessing the evolving course of the disease in addition to the pandemic. Given the partially controlled course of the COVID-19 pandemic, the greatest challenge currently lies in managing the short- and long-term complications of COVID-19. We have assembled current knowledge of the broad spectrum of cardiovascular, pulmonary, and neuropsychiatric sequelae following SARS-CoV-2 infection to understand how these clinical manifestations collectively lead to a severe form of the disease. The ultimate goal would be to better understand these complications and find ways to prevent clinical deterioration.

Published
2022
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39. Assessment of the Mineral Composition and the Selected Physicochemical Parameters of Dietary Supplements Containing Green Tea Extracts.

Author

Ośko J, Szewczyk A, Berk P, Prokopowicz M, and Grembecka M

Abstract

Our aim was to assess the mineral composition as well as the physicochemical quality of green tea-based dietary supplements (capsules and tablets) with respect to average weight, size and shape, friability, breaking force and disintegration time. Products were analysed by flame atomic absorption spectrometry for Ca, K, Mg, Na, Cr, Cu, Fe, Zn, Mn, Pb and Cd. Approximately 60% of the analysed supplements met the requirements of European and Polish regulations. The dietary supplements passed weight variation tests, but not all products had compliant capsule sizes. One product in tablet form failed the friability test, and eleven dietary supplements in tablet form failed the disintegration test. The supplements were characterised by a varied elemental composition, with the highest values found for Ca, Fe, Zn and Mn. The recommended daily allowance realisation for Fe and Zn in two dietary supplements (capsule form) exceeded 100%. As a result of the estimation of the monthly intake of toxic metals, it was concluded that the tested products do not pose a risk to consumer health. Significant relationships (p < 0.001) were found for K, Mg, Na, Cu, Fe, Mn and Zn. The application of factor and cluster analyses allowed the verification of the similarity of green tea extracts contained in dietary supplements to the natural raw material, green tea leaves, used as a reference material.

Published
2022
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40. Antioxidant-Loaded Mesoporous Silica-An Evaluation of the Physicochemical Properties.

Author

Szewczyk A, Brzezińska-Rojek J, Ośko J, Majda D, Prokopowicz M, and Grembecka M

Abstract

The dangerous effects of oxidative stress can be alleviated by antioxidants—substances with the ability to prevent damage caused by reactive oxygen species. The adsorption of antioxidants onto nanocarriers is a well-known method that might protect them against rough environ-mental conditions. The aim of this study was to investigate the adsorption and desorption of gallic acid (GA), protocatechuic acid (PCA), chlorogenic acid (CGA), and 4-hydroxybenzoic acid (4-HBA) using commercially available mesoporous silica materials (MSMs), both parent (i.e., SBA-15 and MCM-41) and surface functionalized (i.e., SBA-NH2 and SBA-SH). The MSMs loaded with active compounds were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), thermoporometry (TPM), and powder X-ray diffraction (XRD). High-performance liquid chromatography (HPLC-CAD) was used to evaluate the performance of the adsorption and desorption processes. The antioxidant potential was investigated using the Folin−Ciocalteu (FC) spectrophotometric method. Among the studied MSMs, the highest adsorption of GA was observed for amine-modified SBA-15 mesoporous silica. The adsorption capacity of SBA-NH2 increased in the order of PCA, 4-HBA < GA < CGA. Different desorption effectiveness levels of the adsorbed compounds were observed with the antioxidant capacity preserved for all investigated compounds.

Published
2022
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41. Mineral Composition of Dietary Supplements-Analytical and Chemometric Approach.

Author

Brzezińska-Rojek J, Rutkowska M, Brzezicha J, Konieczka P, Prokopowicz M, and Grembecka M

Subjects
Dietary Supplements toxicity, Humans, Risk Factors, Beta vulgaris chemistry, Chemometrics methods, Dietary Supplements analysis, Minerals chemistry, Plant Roots chemistry
Abstract

There is a lack of data on the actual composition and effectiveness of beetroot-based dietary supplements. The research aimed to determine the profile of 22 elements (Na, K, Ca, Mg, P, Fe, As, Se, Zn, Cu, Ag, Co, Ni, Mo, Al, Mn, Sr, Cr, Ba, Li, Pb, Cd) in beetroot and its supplements by the microwave plasma atomic emission spectrometry (MP-AES) method. The analytical procedure was optimised and validated. The composition of both groups was compared, assessing compliance with the recommended daily doses for the chosen elements, and the health risk was estimated. Furthermore, chemometric analysis was applied. Beetroots constituted a significant source of elements, especially K, Na, Mg, Ca, P, in contrast to supplements which contained their negligible amounts except from iron-enriched products which provided notable amounts of Fe (38.3-88% of the Recommended Dietary Allowance for an adult male from 19 to 75 years old). Some products were significantly contaminated with toxic elements (As, Cd). Factor and cluster analyses were helpful in the differentiation of beetroot and its supplements in view of their type (vegetable, supplement, iron-enriched supplement), origin, type of cultivation (conventional, organic), and form (capsule, tablet) based on their mineral composition. The obtained results indicate the need for more stringent control of supplements, as they may pose a significant health risk to consumers.

Published
2021
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42. Evaluation of Physicochemical Properties of Beetroot-Based Dietary Supplements.

Author

Brzezińska J, Szewczyk A, Brzezicha J, Prokopowicz M, and Grembecka M

Abstract

In the European Union, no specific requirements for the physicochemical parameters of dietary supplements have been established, contrary to the United States of America. This research aimed to assess the selected physical parameters of 31 commercially available beetroot-based dietary supplements in the form of tablets and capsules following the United States Pharmacopoeia (USP) guidelines and the Food and Drug Administration (FDA) recommendations. There was also estimated zinc and iron content by atomic absorption spectroscopy with flame detection. Results showed that nine products did not meet the USP requirements. Seven supplements needed more than 30 min to disintegrate. Two products in the form of tablets did not pass the friability test because of cracking. The hardness values varied significantly between manufacturers, demonstrating values from 59.1 to 455.8 N. The iron-enriched supplements differed significantly in iron content compared with the manufacturers' declaration (84.91-140.69%). Inappropriate quality of dietary supplements, which may constitute a potential risk to consumers, can be related to the lack of specific regulations in Europe; hence, similar to the USA requirements should be considered in the European Union. The work emphasizes the need to better control the quality of dietary supplements before they are introduced to the European market.

Published
2021
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43. Molecular Mechanism of Thymidylate Synthase Inhibition by N 4 -Hydroxy-dCMP in View of Spectrophotometric and Crystallographic Studies.

Author

Maj P, Jarmuła A, Wilk P, Prokopowicz M, Rypniewski W, Zieliński Z, Dowierciał A, Bzowska A, and Rode W

Subjects
Animals, Crystallography, X-Ray, Deoxycytidine Monophosphate chemistry, Deoxycytidine Monophosphate pharmacology, Enzyme Inhibitors chemistry, Humans, Mice, Molecular Docking Simulation, Spectrophotometry, Thymidylate Synthase chemistry, Thymidylate Synthase metabolism, Trichinellosis parasitology, Deoxycytidine Monophosphate analogs & derivatives, Enzyme Inhibitors pharmacology, Thymidylate Synthase antagonists & inhibitors, Trichinella enzymology
Abstract

Novel evidence is presented allowing further clarification of the mechanism of the slow-binding thymidylate synthase (TS) inhibition by N 4 -hydroxy-dCMP (N 4 -OH-dCMP). Spectrophotometric monitoring documented time- and temperature-, and N 4 -OH-dCMP-dependent TS-catalyzed dihydrofolate production, accompanying the mouse enzyme incubation with N 4 -OH-dCMP and N 5,10 -methylenetetrahydrofolate, known to inactivate the enzyme by the covalent binding of the inhibitor, suggesting the demonstrated reaction to be uncoupled from the pyrimidine C(5) methylation. The latter was in accord with the hypothesis based on the previously presented structure of mouse TS (cf. PDB ID: 4EZ8), and with conclusions based on the present structure of the parasitic nematode Trichinella spiralis , both co-crystallized with N 4 -OH-dCMP and N 5,10 -methylenetetrahdrofolate. The crystal structure of the mouse TS-N 4 -OH-dCMP complex soaked with N 5,10 -methylenetetrahydrofolate revealed the reaction to run via a unique imidazolidine ring opening, leaving the one-carbon group bound to the N(10) atom, thus too distant from the pyrimidine C(5) atom to enable the electrophilic attack and methylene group transfer.

Published
2021
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44. Mesoporous Silica-Bioglass Composite Pellets as Bone Drug Delivery System with Mineralization Potential.

Author

Szewczyk A, Skwira A, Konopacka A, Sądej R, and Prokopowicz M

Subjects
Adsorption, Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents pharmacokinetics, Bone Regeneration, Bone Substitutes pharmacokinetics, Calcification, Physiologic, Calorimetry, Differential Scanning, Doxycycline administration & dosage, Doxycycline pharmacokinetics, Drug Carriers administration & dosage, Drug Carriers chemistry, Humans, In Vitro Techniques, Materials Testing, Microscopy, Electron, Scanning, Particle Size, Regenerative Medicine, Spectrometry, X-Ray Emission, Spectroscopy, Fourier Transform Infrared, Staphylococcus aureus drug effects, Bone Substitutes administration & dosage, Bone Substitutes chemistry, Ceramics chemistry, Drug Delivery Systems, Silicon Dioxide administration & dosage, Silicon Dioxide chemistry
Abstract

For decades, local bone drug delivery systems have been investigated in terms of their application in regenerative medicine. Among them, inorganic polymers based on amorphous silica have been widely explored. In this work, we combined two types of amorphous silica: bioglass and doxycycline-loaded mesoporous silica MCM-41 into the form of spherical granules (pellets) as a bifunctional bone drug delivery system. Both types of silica were obtained in a sol-gel method. The drug adsorption onto the MCM-41 was performed via adsorption from concentrated doxycycline hydrochloride solution. Pellets were obtained on a laboratory scale using the wet granulation-extrusion-spheronization method and investigated in terms of physical properties, drug release, antimicrobial activity against Staphylococcus aureus , mineralization properties in simulated body fluid, and cytotoxicity towards human osteoblasts. The obtained pellets were characterized by satisfactory mechanical properties which eliminated the risk of pellets cracking during further investigations. The biphasic drug release from pellets was observed: burst stage (44% of adsorbed drug released within the first day) followed by prolonged release with zero-order kinetics (estimated time of complete drug release was 19 days) with maintained antimicrobial activity. The progressive biomimetic apatite formation on the surface of the pellets was observed. No cytotoxic effect of pellets towards human osteoblasts was noticed.

Published
2021
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45. Advanced Spectroscopy and APBS Modeling for Determination of the Role of His190 and Trp103 in Mouse Thymidylate Synthase Interaction with Selected dUMP Analogues.

Author

Prokopowicz M, Jarmuła A, Casamayou-Boucau Y, Gordon F, Ryder A, Sobich J, Maj P, Cieśla J, Zieliński Z, Fita P, and Rode W

Subjects
Amino Acid Substitution, Animals, Deoxycytidine Monophosphate analogs & derivatives, Deoxycytidine Monophosphate metabolism, Deoxyuracil Nucleotides chemistry, Fluorodeoxyuridylate metabolism, Mice, Models, Molecular, Multivariate Analysis, Protein Conformation, Thymidylate Synthase chemistry, Deoxyuracil Nucleotides metabolism, Models, Theoretical, Spectrometry, Fluorescence methods, Static Electricity, Thymidylate Synthase metabolism
Abstract

A homo-dimeric enzyme, thymidylate synthase (TS), has been a long-standing molecular target in chemotherapy. To further elucidate properties and interactions with ligands of wild-type mouse thymidylate synthase (mTS) and its two single mutants, H190A and W103G, spectroscopic and theoretical investigations have been employed. In these mutants, histidine at position 190 and tryptophan at position 103 are substituted with alanine and glycine, respectively. Several emission-based spectroscopy methods used in the paper demonstrate an especially important role for Trp 103 in TS ligands binding. In addition, the Advanced Poisson-Boltzmann Solver (APBS) results show considerable differences in the distribution of electrostatic potential around Trp 103, as compared to distributions observed for all remaining Trp residues in the mTS family of structures. Together, spectroscopic and APBS results reveal a possible interplay between Trp 103 and His190, which contributes to a reduction in enzymatic activity in the case of H190A mutation. Comparison of electrostatic potential for mTS complexes, and their mutants, with the substrate, dUMP, and inhibitors, FdUMP and N4-OH-dCMP, suggests its weaker influence on the enzyme-ligand interactions in N4OH-dCMP-mTS compared to dUMP-mTS and FdUMP-mTS complexes. This difference may be crucial for the explanation of the "abortive reaction" inhibitory mechanism of N4OH-dCMP towards TS. In addition, based on structural analyses and the H190A mutant capacity to form a denaturation-resistant complex with N4-OH-dCMP in the mTHF-dependent reaction, His190 is apparently responsible for a strong preference of the enzyme active center for the anti rotamer of the imino inhibitor form.

Published
2021
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46. The Links between Cardiovascular Diseases and Alzheimer's Disease.

Author

Leszek J, Mikhaylenko EV, Belousov DM, Koutsouraki E, Szczechowiak K, Kobusiak-Prokopowicz M, Mysiak A, Diniz BS, Somasundaram SG, Kirkland CE, and Aliev G

Subjects
Genome-Wide Association Study, Humans, Inflammation, Alzheimer Disease epidemiology, Cardiovascular Diseases epidemiology, Gastrointestinal Microbiome
Abstract

The root cause of non-inherited Alzheimer's disease (AD) remains unknown despite hundreds of research studies performed to attempt to solve this problem. Since proper prophylaxis remains the best strategy, many scientists have studied the risk factors that may affect AD development. There is robust evidence supporting the hypothesis that cardiovascular diseases (CVD) may contribute to AD progression, as the diseases often coexist. Therefore, a lack of well-defined diagnostic criteria makes studying the relationship between AD and CVD complicated. Additionally, inflammation accompanies the pathogenesis of AD and CVD, and is not only a consequence but also implicated as a significant contributor to the course of the diseases. Of note, АроЕε4 is found to be one of the major risk factors affecting both the cardiovascular and nervous systems. According to genome wide association and epidemiological studies, numerous common risk factors have been associated with the development of AD-related pathology. Furthermore, the risk of developing AD and CVDs appears to be increased by a wide range of conditions and lifestyle factors: hypertension, dyslipidemia, hypercholesterolemia, hyperhomocysteinemia, gut/oral microbiota, physical activity, and diet. This review summarizes the literature and provides possible mechanistic links between CVDs and AD., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Published
2021
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47. Relationships between Circulating Matrix Metalloproteinases, Tissue Inhibitor TIMP-2, and Renal Function in Patients with Myocarditis.

Author

Kobusiak-Prokopowicz M, Kaaz K, Marciniak D, Karolko B, and Mysiak A

Subjects
Adult, Female, Glomerular Filtration Rate, Humans, Kidney physiopathology, Male, Myocarditis complications, Myocarditis physiopathology, Renal Insufficiency, Chronic complications, Renal Insufficiency, Chronic physiopathology, Matrix Metalloproteinase 2 blood, Matrix Metalloproteinase 3 blood, Matrix Metalloproteinase 9 blood, Myocarditis blood, Renal Insufficiency, Chronic blood, Tissue Inhibitor of Metalloproteinase-2 blood
Abstract

Introduction: Under physiological conditions, the myocardial extracellular matrix (ECM) is maintained by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). However, changes in the balance between MMPs and TIMPs can lead to pathological remodeling of the ECM, which contributes to cardiovascular and kidney diseases. The aim of our study was to assess levels of MMPs and TIMP-2 in patients with myocarditis and their relationship to renal function., Materials and Methods: Forty five patients with myocarditis who underwent CMR were included, comprising 11 with concurrent chronic kidney disease (CKD). Blood samples were obtained to assess serum levels of MMP-2, MMP-3, MMP-9, and TIMP-2., Results: Serum MMP-2, MMP-3, and TIMP-2 levels negatively correlated with the ejection fraction in patients with myocarditis, while MMP-3 levels correlated with longitudinal deformation (p < 0.05). Serum MMP-2, MMP-3, and TIMP-2 levels also negatively correlated with renal function, as assessed by the estimated glomerular filtration rate (eGFR) (p < 0.05). Patients with myocarditis and concurrent CKD had higher levels of MMP-2 and TIMP-2 than those without kidney damage., Conclusions: (1) We demonstrated that MMP-2, MMP-3, and TIMP-2 concentrations were related to left-ventricular ejection fraction, and MMP-3 levels correlated with longitudinal deformation, indicating MMPs play an important role in the post-inflammatory remodeling of the myocardium. (2) A negative correlation between the eGFR and MMP-2, MMP-3, and TIMP-2 and a positive correlation between creatinine and MMP-3 levels indicate the role of MMPs and TIMP-2 in renal dysfunction., (© 2021 The Author(s). Published by S. Karger AG, Basel.)

Published
2021
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48. Microwave-Assisted Fabrication of Mesoporous Silica-Calcium Phosphate Composites for Dental Application.

Author

Szewczyk A, Skwira A, Ginter M, Tajer D, and Prokopowicz M

Abstract

Herein, the microwave-assisted wet precipitation method was used to obtain materials consisting of mesoporous silica (SBA-15) and calcium orthophosphates (CaP). Composites were prepared through immersion of mesoporous silica in different calcification coating solutions and then exposed to microwave radiation. The composites were characterized in terms of molecular structure, crystallinity, morphology, chemical composition, and mineralization potential by Fourier-transform infrared spectroscopy (FTIR), powder X-ray diffraction (XRD), and scanning electron microscopy equipped with energy-dispersive X-ray spectroscopy (SEM-EDX). The application of microwave irradiation resulted in the formation of different types of calcium orthophosphates such as calcium deficient hydroxyapatite (CDHA), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP) on the SBA-15 surface, depending on the type of coating solution. The composites for which the progressive formation of hydroxyapatite during incubation in simulated body fluid was observed were further used in the production of final pharmaceutical forms: membranes, granules, and pellets. All of the obtained pharmaceutical forms preserved mineralization properties.

Published
2020
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49. Response to antiplatelet therapy in patients undergoing invasive treatment due to acute coronary syndrome complicated by cardiogenic shock.

Author

Kuliczkowski W, Cielecka-Prynda M, Karolko B, Kaaz K, Adamik B, Bednarczyk D, Kobusiak-Prokopowicz M, and Mysiak A

Abstract

Introduction: There are limited data on platelet reactivity and response to antiplatelet drugs in patients with cardiogenic shock., Aim: To assess platelet reactivity on dual antiplatelet therapy with acetylsalicylic acid (ASA) and ticagrelor, a novel potent P2Y12 receptor inhibitor, in patients with cardiogenic shock in the course of acute coronary syndrome (ACS) who received invasive treatment., Material and Methods: We enrolled 12 consecutive patients with ACS complicated by cardiogenic shock. To assess response to antiplatelet therapy during cardiogenic shock, only patients with symptoms persisting for at least 3 days and who completed a 5-day follow-up were included in the study. Patients received a loading dose of ASA (300 mg) and ticagrelor (180 mg), followed by a maintenance dose (ASA, 1 × 75 mg; ticagrelor, 2 × 90 mg). Blood samples for platelet function tests were collected. Platelet aggregation was assessed with a Multiplate whole-blood impedance aggregometer. Arachidonic acid (AA), adenosine diphosphate (ADP), and thrombin receptor-activating peptide (TRAP) were used as aggregation agonists., Results: Response to antiplatelet therapy assessed by aggregometry showed numerically higher on-ASA platelet reactivity on day one and statistically significant higher on-ticagrelor platelet reactivity on day one in comparison with following days. There were 2 patients with high on ASA platelet reactivity and 3 with high on ticagrelor platelet reactivity, but only on the day one., Conclusions: Some patients with cardiogenic shock in the course of ACS treated invasively show a lower response to ASA and ticagrelor only on the first day after invasive treatment, with a good response on subsequent days., Competing Interests: The authors declare no conflict of interest., (Copyright © 2020 Termedia Sp. z o. o.)

Published
2020
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50. Mesoporous silica pellets as bifunctional bone drug delivery system for cefazolin.

Author

Szewczyk A, Skwira A, Konopacka A, Sądej R, Walker G, and Prokopowicz M

Subjects
Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents toxicity, Cefazolin administration & dosage, Cefazolin toxicity, Cell Line, Delayed-Action Preparations, Drug Compounding, Drug Implants, Drug Liberation, Durapatite chemistry, Hardness, Humans, Kinetics, Osteoblasts drug effects, Osteomyelitis drug therapy, Osteomyelitis microbiology, Porosity, Silicon Dioxide toxicity, Staphylococcus aureus drug effects, Staphylococcus aureus growth & development, Surface Properties, Anti-Bacterial Agents chemistry, Cefazolin chemistry, Drug Carriers, Silicon Dioxide chemistry
Abstract

For decades, bone drug delivery systems dedicated for osteomyelitis treatment have been investigated as bifunctional materials that exhibit prolonged drug release and mineralization potential. Herein, composite-type pellets based on cefazolin-loaded amino-modified mesoporous silica SBA-15 and microwave-assisted hydroxyapatite were investigated as potential bone drug delivery system in vitro. Pellets were obtained by granulation, extrusion and spheronization methods in laboratory scale and studied in terms of physical properties, drug release, mineralization potential, antimicrobial activity and cytotoxicity towards human osteoblasts. The obtained pellets were characterized for hardness and friability which indicated the pellets durability during further investigations. Prolonged (5-day) release of cefazolin from pellets was observed. The pellets exhibited mineralization potential in simulated body fluid, i.e., a continuous layer of bone-like apatite was formed on the surface of pellets after 28 days of incubation. An antimicrobial assay of pellets revealed an antibacterial effect against Staphylococcus aureus strain during 6 days. No cytotoxic effects of pellets towards human osteoblasts were observed. The obtained results proved that proposed pellets appear to have potential applications as bone drug delivery systems., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Published
2020
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