Search

Metagenomics, the integral study of all organisms present in the selected environment, has rapidly advanced with the advent of next generation sequencing (NGS) methods. Among the key parameters of NGS that affect the reliability of the results are read length, sequencing depth and read accuracy. In this review, we depict the essential NGS parameters in metagenomic projects and strategies to improve their reliability. We then further discuss the parameters and strategies along two recent case studies - determination of human oral microbiome and mining of novel genes for thermo-stable degradation of cellulose. [ABSTRACT FROM AUTHOR]