118 results on '"Prigent-Combaret C"'
Search Results
2. Azospirillum -Plant Interaction
- Author
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Wisniewski-Dyé, F, primary, Drogue, B, additional, Borland, S, additional, and Prigent-Combaret, C, additional
- Published
- 2013
- Full Text
- View/download PDF
3. Assessment of SCAR markers to design real-time PCR primers for rhizosphere quantification of Azospirillum brasilense phytostimulatory inoculants of maize
- Author
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Couillerot, O., Poirier, M.-A., Prigent-Combaret, C., Mavingui, P., Caballero-Mellado, J., and Moënne-Loccoz, Y.
- Published
- 2010
- Full Text
- View/download PDF
4. Survival and spread of Shiga toxin-producing Escherichia coli in alpine pasture grasslands
- Author
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Fremaux, B., Prigent-Combaret, C., Beutin, L., Gleizal, A., Trevisan, D., Quetin, P., Jocteur-Monrozier, L., and Rozand, C.
- Published
- 2010
- Full Text
- View/download PDF
5. Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens
- Author
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Couillerot, O., Prigent-Combaret, C., Caballero-Mellado, J., and Moënne-Loccoz, Y.
- Published
- 2009
- Full Text
- View/download PDF
6. Persistence of Shiga toxin-producing Escherichia coli O26 in various manure-amended soil types
- Author
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Fremaux, B., Prigent-Combaret, C., Delignette-Muller, M. L., Mallen, B., Dothal, M., Gleizal, A., and Vernozy-Rozand, C.
- Published
- 2008
7. Persistence of Shiga toxin-producing Escherichia coli O26 in cow slurry
- Author
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Fremaux, B., Prigent-Combaret, C., Delignette-Muller, M. L., Dothal, M., and Vernozy-Rozand, C.
- Published
- 2007
8. Growth and survival of non-O157:H7 Shiga-toxin-producing Escherichia coli in cow manure
- Author
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Fremaux, B., Delignette-Muller, M. L., Prigent-Combaret, C., Gleizal, A., and Vernozy-Rozand, C.
- Published
- 2007
9. Freshwater selenium-methylating bacterial thiopurine methyltransferases: diversity and molecular phylogeny
- Author
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Favre-Bonté, S., Ranjard, L., Colinon, C., Prigent-Combaret, C., Nazaret, S., and Cournoyer, B.
- Published
- 2005
10. The bacterial thiopurine methyltransferase tellurite resistance process is highly dependent upon aggregation properties and oxidative stress response
- Author
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Prigent-Combaret, C., Sanguin, H., Champier, L., Bertrand, C., Monnez, Claire, Colinon-Dupuich, C., Blaha, D., Ghigo, J. M., Cournoyer, Benoit, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Génétique des Biofilms, Institut Pasteur [Paris], French National Research Agency (ANR) ANR, Contaminants et Environnements: Metrologie, Sante, Adaptabilite, Comportements et Usages' (CESA), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)
- Subjects
[SDV]Life Sciences [q-bio] ,Methylation ,Gene Knockout Techniques ,Toxicité ,Drug Resistance, Bacterial ,tellurite de potassium ,Escherichia coli ,Expression des gènes ,Oxydation ,Enzyme Inhibitors ,Adhesins, Bacterial ,Résistance aux produits chimiques ,Bacteria ,Gene Expression Profiling ,Volatilisation ,000 - Autres thèmes ,stress oxydatif ,Gene Expression Regulation, Bacterial ,Methyltransferases ,Oxidative Stress ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Genes, Bacterial ,Mutation ,Tellurium ,Activité enzymatique ,Oxidation-Reduction ,P02 - Pollution - Abstract
International audience; Bacterial thiopurine methyltransferases (bTPMTs) can favour resistance towards toxic tellurite oxyanions through a pathway leading to the emission of a garlic-like smell. Gene expression profiling completed by genetic, physiological and electron microscopy analyses was performed to identify key bacterial activities contributing to this resistance process. Escherichia coli strain MG1655 expressing the bTPMT was used as a cell model in these experiments. This strain produced a garlic-like smell which was found to be due to dimethyl telluride, and cell aggregates in culture media supplemented with tellurite. Properties involved in aggregation were correlated with cell attachment to polystyrene, which increased with tellurite concentrations. Gene expression profiling supported a role of adhesins in the resistance process with 14% of the tellurite-regulated genes involved in cell envelope, flagella and fimbriae biogenesis. Other tellurite-regulated genes were, at 27%, involved in energy, carbohydrate and amino acid metabolism including the synthesis of antioxidant proteins, and at 12% in the synthesis of transcriptional regulators and signal transduction systems. Escherichia coli mutants impaired in tellurite-regulated genes showed ubiquinone and adhesins synthesis, oxidative stress response, and efflux to be essential in the bTPMT resistance process. High tellurite resistance required a synergistic expression of these functions and an efficient tellurium volatilization by the bTPMT.
- Published
- 2012
11. The Pseudomonas secondary metabolite 2, 4-diacetylphloroglucinol is a signal inducing rhizoplane expression of Azospirillum genes involved in plant-growth promotion
- Author
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Combes-Meynet, E., Pothier, J. F., Moënne-Loccoz, Yvan, Prigent-Combaret, C., Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL)
- Subjects
[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2011
12. Rôle des facteurs édaphiques et hydrométéorologiques dans la survie et le transfert de bactéries fécales bovines, à l'échelle bassin versant: cas de pâturages d'altitude
- Author
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Dorioz, J. M., Quetin, P., Prigent-Combaret, C., Trévisan, D., Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL), Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL)
- Subjects
alpages ,alpage de Bise ,[SDV]Life Sciences [q-bio] ,fecal bacteria ,cuenca vertiente ,escherichia coli ,pastos ,bacterias fecales ,bassin versant ,rangeland ,bactéries fécales ,watershed - Abstract
This research project entitled « Pastor » is devoted to the study of the survival and dissemination of fecal bacteria contaminants originated from cattle, in soil and surface water. It focuses on microbial contamination by Escherichia coli in relationship with dairy grazing of alpine rangelands. E. coli is considered as a global indicator of fecal contaminations. Studies have been carried both at the plot and at the watershed scale. They deal with : 1) the dynamics of E. coli populations, including pathogenic STEC (Shiga-toxin producing E. coli) strains, released in the mountain environment ; the objective is to characterize survival and storage ompartments of E. coli in soil following their release by cattle feces ; the population dynamics of E. coli in soils is compared with dynamics of typical soil inhabitants (the fluorescent Pseudomonas spp.), in order to reveal specific adaptive strategies of E. coli ;2) the identification and characterization of the grazing areas providing specifically a high contribution to fecal contamination of surface water ; this was carried out by quantifying and modeling E. coli transfers from soil to water, with the aim to link together E. coli fluxes monitored at the watershed outlet, grazing conditions and hydrologic properties of the watershed. The output of the model is of interest to identify the critical source areas. Further assessment of the groundwater compartment is needed to improve our understanding of E. coli ecology especially with regards to the outlet peaks unrelated to stromflows. The operational interest of « Pastor » concerns the prevention of fecal pollution of surface water and the relevance of E. coli as an indicator of fecal contamination. The knowledge of critical source areas is an important information for management of pastured watersheds.; El proyecto “Pastor” trata de la supervivencia y de la diseminación de los contaminantes microbianos del suelo hacia las aguas. Apunta la contaminación microbiana del agua por E. coli (indicador de la contaminación fecal) en los prados alpestres en relación con los pastos de lechera. La demanda social en términos de calidad microbiana del agua está importante, incluyendo los Alpes norteños franceses, región de reserva de agua. Las contaminaciones se deben en parte a las prácticas de pastizales. Un inquietante desenlace está la ocurrencia de animales portadores sanos de E. coli potencialmente patogénicos para los humanos y cuya diseminación por los animales de granja está posible. La calidad del agua en las zonas de pastizales entra en debates más amplios sobre la gestión sostenible de los recursos de aguas subterráneas bajo pastos. Las investigaciones desarrollas en el proyecto “Pastor” tratan de : 1) la dinámica de las populaciones de E. coli, incluyendo las tensiones debidas a las STEC patogénicas, una vez liberadas en el medio ambiente montañoso ; el objetivo está caracterizar los compartimentos de supervivencia y de almacenamiento de E. coli en los suelos después de su depósito por las deyecciones del ganado, y comparar con la dinámica de las populaciones típicas del suelo (Pseudomonas spp. fluorecens) para la evaluación de las estrategias adaptivas de E. coli. 2) la identificación y la caracterización de las zonas de pastos que contribuyen a la contaminación fecal de las aguas de superficie en una cuenca vertiente alpestre ; Se midió la cuantidad de E. coli que está transferida de las diferentes zonas de pastos hacia el agua, con la finalidad de ligar el stock de E. coli y el flujo a la salida de la cuenca vertiente, al funcionamiento del pasto y a las propiedades hidrológicas de la cuenca vertiente. “Pastor” incluye también un análisis del funcionamiento de la cuenca vertiente en términos de transferencia de los contaminantes fecales y de modelización del flujo bacteriano. La información necesaria para la modelización incluye los regímenes de exportación a la salida de la cuenca vertiente, condición de transferencia en la cuenca vertiente, y el potencial de liberación de las diferentes unidades de pastos. El rendimiento del modelo estará importante para la gestión mejorada de los recursos en agua en los pastos alpestres. La significación práctica de “Pastor” concierne la prevención de la contaminación fecal del agua y la pertinencia de E. coli como indicador de la contaminación fecal. Es necesario tener una evaluación más precisa del compartimento del agua subterránea para mejorar nuestro conocimiento de la ecología de E. coli especialmente para los picos de salida no ligados a los flujos. El conocimiento de las zonas de contaminación activa está importante para la gestión de los circuitos de pastos, de las zonas de abrevadero del ganado, de los pasos de arroyos, así como el cierre de las zonas de riesgo. Estas medidas de protección están más bien costosas a aplicar. Sin embargo, a pesar del hecho que una proporción muy pequeña de contaminantes microbianos llega al agua de superficie, pueden tener consecuencias muy significativas en términos de salud humana y animal.; L’objectif de ce projet mené dans le cadre du programme GESSOL 2 est de comprendre et modéliser la survie et la dynamique de transfert dans les déjections, les sols et les eaux, de contaminants fécaux bovins, en relation avec les pratiques pastorales et la variabilité des milieux. Les suivis sont centrés sur Escherichia coli et incluent des données sur les souches pathogènes STEC (Shiga-toxin producing E. coli). Des suivis complémentaires portant sur Pseudomonas spp fluorescents, populations typiques des sols, fournissent des éléments de comparaison importants. Les recherches sont réalisées dans un alpage des Alpes du nord, à l’échelle station-parcelle et bassin versant. L’article présente une vue d’ensemble de la démarche d’étude, des méthodologies et des résultats obtenus, puis se focalise sur les acquis concernant le comportement de E. coli dans les sols et sur les apports de la modélisation des transferts à l’échelle bassin versant. Les résultats sont discutés dans une double perspective, celle d’une synthèse présentant le fonctionnement du bassin versant pâturé comme un système de transfert de contaminants fécaux et celle de réflexions opérationnelles relatives à la gestion pastorale.
- Published
- 2011
13. Duplication of plasmid-borne nitrite reductase gene \textitnirK in the wheat-associated plant growth-promoting rhizobacterium \textitAzospirillum brasilense Sp245
- Author
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Pothier, Joel F., Prigent-Combaret, C., Haurat, J., Moënne-Loccoz, Y., Wisniewski-Dyé, F., Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)
- Subjects
[SDV]Life Sciences [q-bio] - Published
- 2008
14. Persistence of Shiga toxin-producing \textitEscherichia coli O26 in various manure-amended soil types
- Author
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Fremaux, B., Prigent-Combaret, C., Delignette-Muller, M. L., Mallen, B., Dothal, M., Gleizal, A., Vernozy-Rozand, C., Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)
- Subjects
[SDV]Life Sciences [q-bio] - Published
- 2008
15. Persistence of Culturable Fecal Contaminants in Dairy Alpine Grassland Soils
- Author
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Texier, S., Prigent-Combaret, C., Gourdon, M. H., Poirier, M. A., Faivre, P., Dorioz, J. M., Poulenard, J., Jocteur-Monrozier, L., Moënne-Loccoz, Y., Trevisan, D., Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), and Lyon 1, Depot 1
- Subjects
[SDV] Life Sciences [q-bio] ,[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2008
16. Physical organization and phylogenetic analysis of acdR as leucine-responsive regulator of the 1-aminocyclopropane-1-carboxylate deaminase gene acdS in phytobeneficial Azospirillum lipoferum 4B and other Proteobacteria
- Author
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Prigent-Combaret, C., Blaha, D., Pothier, Joel F., Vial, L., Poirier, M. A., Wisniewski-Dyé, F., Moënne-Loccoz, Y., Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL)
- Subjects
[SDV]Life Sciences [q-bio] ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2008
17. Survival and spread of Shiga toxin-producingEscherichia coliin alpine pasture grasslands
- Author
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Fremaux, B., primary, Prigent-Combaret, C., additional, Beutin, L., additional, Gleizal, A., additional, Trevisan, D., additional, Quetin, P., additional, Jocteur-Monrozier, L., additional, and Rozand, C., additional
- Published
- 2010
- Full Text
- View/download PDF
18. Pseudomonas fluorescensand closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens
- Author
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Couillerot, O., primary, Prigent-Combaret, C., additional, Caballero-Mellado, J., additional, and Moënne-Loccoz, Y., additional
- Published
- 2009
- Full Text
- View/download PDF
19. Persistence of Shiga toxin-producing Escherichia coli O26 in various manure-amended soil types
- Author
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Fremaux, B., primary, Prigent-Combaret, C., additional, Delignette-Muller, M.L., additional, Mallen, B., additional, Dothal, M., additional, Gleizal, A., additional, and Vernozy-Rozand, C., additional
- Published
- 2007
- Full Text
- View/download PDF
20. Dimethylpolysulfides production as the major mechanism behind wheat fungal pathogen biocontrol, by Arthrobacter and Microbacterium actinomycetes.
- Author
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Ballot A, Dore J, Rey M, Meiffren G, Langin T, Joly P, Dreux-Zigha A, Taibi A, and Prigent-Combaret C
- Subjects
- Microbacterium, Triticum microbiology, Actinomyces, Soil, Plant Diseases prevention & control, Plant Diseases microbiology, Arthrobacter, Actinobacteria
- Abstract
Importance: As the management of wheat fungal diseases becomes increasingly challenging, the use of bacterial agents with biocontrol potential against the two major wheat phytopathogens, Fusarium graminearum and Zymoseptoria tritici , may prove to be an interesting alternative to conventional pest management. Here, we have shown that dimethylpolysulfide volatiles are ubiquitously and predominantly produced by wheat-associated Microbacterium and Arthrobacter actinomycetes, displaying antifungal activity against both pathogens. By limiting pathogen growth and DON virulence factor production, the use of such DMPS-producing strains as soil biocontrol inoculants could limit the supply of pathogen inocula in soil and plant residues, providing an attractive alternative to dimethyldisulfide fumigant, which has many non-targeted toxicities. Notably, this study demonstrates the importance of bacterial volatile organic compound uptake by inhibited F. graminearum , providing new insights for the study of volatiles-mediated toxicity mechanisms within bacteria-fungus signaling crosstalk., Competing Interests: The authors declare no conflict of interest.
- Published
- 2023
- Full Text
- View/download PDF
21. Inhibition of broomrape germination by 2,4-diacetylphloroglucinol produced by environmental Pseudomonas.
- Author
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Lurthy T, Perot S, Gerin-Eveillard F, Rey M, Wisniewski-Dyé F, Vacheron J, and Prigent-Combaret C
- Subjects
- Germination, Plant Weeds, Seeds, Orobanche physiology, Brassica napus
- Abstract
Parasitic weeds such as broomrapes (Phelipanche ramosa and Orobanche cumana) cause severe damage to crops and their development must be controlled. Given that phloroglucinol compounds (PGCs) produced by environmental Pseudomonas could be toxic towards certain plants, we assessed the potential herbicidal effect of the bacterial model Pseudomonas ogarae F113, a PGCs-producing bacterium, on parasitic weed. By combining the use of a mutagenesis approach and of pure PGCs, we evaluated the in vitro effect of PGC-produced by P. ogarae F113 on broomrape germination and assessed the protective activity of a PGC-producing bacteria on oilseed rape (Brassica napus) against P. ramosa in non-sterile soils. We showed that the inhibition of the germination depends on the PGCs molecular structure and their concentrations as well as the broomrape species and pathovars. This inhibition caused by the PGCs is irreversible, causing a brown coloration of the broomrape seeds. The inoculation of PGCs-producing bacteria limited the broomrape infection of P. ramosa, without affecting the host growth. Moreover, elemental profiling analysis of oilseed rape revealed that neither F113 nor applied PGCs affected the nutrition capacity of the oilseed rape host. Our study expands the knowledge on plant-beneficial Pseudomonas as weed biocontrol agents and opens new avenues for the development of natural bioherbicides to enhance crop yield., (© 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
22. Genomic content of wheat has a higher influence than plant domestication status on the ability to interact with Pseudomonas plant growth-promoting rhizobacteria.
- Author
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Gruet C, Alaoui M, Gerin F, Prigent-Combaret C, Börner A, Muller D, and Moënne-Loccoz Y
- Subjects
- Domestication, Plant Roots metabolism, Genomics, Triticum metabolism, Pseudomonas
- Abstract
Plant evolutionary history has had profound effects on belowground traits, which is likely to have impacted the ability to interact with microorganisms, but consequences on root colonization and gene expression by plant growth-promoting rhizobacteria (PGPR) remain poorly understood. Here, we tested the hypothesis that wheat genomic content and domestication are key factors determining the capacity for PGPR interaction. Thus, 331 wheat representatives from eight Triticum or Aegilops species were inoculated under standardized conditions with the generalist PGPR Pseudomonas ogarae F113, using an autofluorescent reporter system for monitoring F113 colonization and expression of phl genes coding for the auxinic inducing signal 2,4-diacetylphloroglucinol. The interaction with P. ogarae F113 was influenced by ploidy level, presence of genomes AA, BB, DD, and domestication. While root colonization was higher for hexaploid and tetraploid species, and phl expression level higher for hexaploid wheat, the diploid Ae. tauschii displayed higher phl induction rate (i.e., expression:colonisation ratio) on roots. However, a better potential of interaction with F113 (i.e., under non-stress gnotobiotic conditions) did not translate, after seed inoculation, into better performance of wheat landraces in non-sterile soil under drought. Overall, results showed that domestication and especially plant genomic content modulate the PGPR interaction potential of wheats., (© 2023 John Wiley & Sons Ltd.)
- Published
- 2023
- Full Text
- View/download PDF
23. Impact of PGPR inoculation on root morphological traits and root exudation in rapeseed and camelina: interactions with heat stress.
- Author
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Delamare J, Brunel-Muguet S, Boukerb AM, Bressan M, Dumas L, Firmin S, Leroy F, Morvan-Bertrand A, Prigent-Combaret C, and Personeni E
- Subjects
- Plant Roots metabolism, Plants, Bacteria, Heat-Shock Response, Brassica napus, Mycorrhizae, Brassica rapa, Alphaproteobacteria
- Abstract
Root exudation is involved in the recruitment of beneficial microorganisms by trophic relationships and/or signalling pathways. Among beneficial microorganisms, Plant Growth-Promoting Rhizobacteria (PGPR) are known to improve plant growth and stress resistance. These interactions are of particular importance for species that do not interact with mycorrhizal fungi, such as rapeseed (Brassica napus L.) and camelina (Camelina sativa (L.) Crantz). However, heat stress is known to have a quantitative and qualitative impact on root exudation and could affect the interactions between plants and PGPR. We aimed to analyse the effects of PGPR inoculation on root morphology and exudation in rapeseed and camelina at the reproductive stage. The modulation of the effects of these interactions under heat stress was also investigated. The plants were inoculated twice at the reproductive stage with two different Pseudomonas species and were exposed to heat stress after the second inoculation. In non-stressing conditions, after bacterial inoculation, rapeseed and camelina exhibited two contrasting behaviours in C root allocation. While rapeseed plants seemed to suffer from the interactions with the bacteria, camelina plants appeared to control the relationship with the PGPR by modifying the composition of their root exudates. Under heat stress, the plant-PGPR interaction was unbalanced for rapeseed, for which the C allocation strategy is mainly driven by the C cost from the bacteria. Alternatively, camelina plants prioritized C allocation for their own above-ground development. This work opens up new perspectives for understanding plant-PGPR interactions, especially in an abiotic stress context., (© 2023 Scandinavian Plant Physiology Society.)
- Published
- 2023
- Full Text
- View/download PDF
24. Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing Azospirillum PGPR.
- Author
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Valente J, Gerin F, Mini A, Richard R, Le Gouis J, Prigent-Combaret C, and Moënne-Loccoz Y
- Abstract
Crop varieties differ in their ability to interact with Plant Growth-Promoting Rhizobacteria (PGPR), but the genetic basis for these differences is unknown. This issue was addressed with the PGPR Azospirillum baldaniorum Sp245, using 187 wheat accessions. We screened the accessions based on the seedling colonization by the PGPR and the expression of the phenylpyruvate decarboxylase gene ppdC (for synthesis of the auxin indole-3-acetic acid), using gusA fusions. Then, the effects of the PGPR on the selected accessions stimulating Sp245 (or not) were compared in soil under stress. Finally, a genome-wide association approach was implemented to identify the quantitative trait loci (QTL) associated with PGPR interaction. Overall, the ancient genotypes were more effective than the modern genotypes for Azospirillum root colonization and ppdC expression. In non-sterile soil, A. baldaniorum Sp245 improved wheat performance for three of the four PGPR-stimulating genotypes and none of the four non-PGPR-stimulating genotypes. The genome-wide association did not identify any region for root colonization but revealed 22 regions spread on 11 wheat chromosomes for ppdC expression and/or ppdC induction rate. This is the first QTL study focusing on molecular interaction with PGPR bacteria. The molecular markers identified provide the possibility to improve the capacity of modern wheat genotypes to interact with Sp245, as well as, potentially, other Azospirillum strains.
- Published
- 2023
- Full Text
- View/download PDF
25. Dark-zone alterations expand throughout Paleolithic Lascaux Cave despite spatial heterogeneity of the cave microbiome.
- Author
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Bontemps Z, Prigent-Combaret C, Guillmot A, Hugoni M, and Moënne-Loccoz Y
- Abstract
Background: Cave anthropization related to rock art tourism can lead to cave microbiota imbalance and microbial alterations threatening Paleolithic artwork, but the underpinning microbial changes are poorly understood. Caves can be microbiologically heterogeneous and certain rock wall alterations may develop in different rooms despite probable spatial heterogeneity of the cave microbiome, suggesting that a same surface alteration might involve a subset of cosmopolitan taxa widespread in each cave room. We tested this hypothesis in Lascaux, by comparing recent alterations (dark zones) and nearby unmarked surfaces in nine locations within the cave., Results: Illumina MiSeq metabarcoding of unmarked surfaces confirmed microbiome heterogeneity of the cave. Against this background, the microbial communities of unmarked and altered surfaces differed at each location. The use of a decision matrix showed that microbiota changes in relation to dark zone formation could differ according to location, but dark zones from different locations displayed microbial similarities. Thus, dark zones harbor bacterial and fungal taxa that are cosmopolitan at the scale of Lascaux, as well as dark zone-specific taxa present (i) at all locations in the cave (i.e. the six bacterial genera Microbacterium, Actinophytocola, Lactobacillus, Bosea, Neochlamydia and Tsukamurella) or (ii) only at particular locations within Lascaux. Scanning electron microscopy observations and most qPCR data evidenced microbial proliferation in dark zones., Conclusion: Findings point to the proliferation of different types of taxa in dark zones, i.e. Lascaux-cosmopolitan bacteria and fungi, dark zone-specific bacteria present at all locations, and dark zone-specific bacteria and fungi present at certain locations only. This probably explains why dark zones could form in various areas of the cave and suggests that the spread of these alterations might continue according to the area of distribution of key widespread taxa., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
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26. The application of plant growth-promoting rhizobacteria in Solanum lycopersicum production in the agricultural system: a review.
- Author
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Adedayo AA, Babalola OO, Prigent-Combaret C, Cruz C, Stefan M, Kutu F, and Glick BR
- Subjects
- Humans, Animals, Agriculture, Plant Development, Plants microbiology, Bacteria, Soil chemistry, Solanum lycopersicum, Alphaproteobacteria
- Abstract
Food safety is a significant challenge worldwide, from plantation to cultivation, especially for perishable products such as tomatoes. New eco-friendly strategies are needed, and beneficial microorganisms might be a sustainable solution. This study demonstrates bacteria activity in the tomato plant rhizosphere. Further, it investigates the rhizobacteria's structure, function, and diversity in soil. Rhizobacteria that promote the growth and development of tomato plants are referred to as plant growth-promoting bacteria (PGPR). They form a series of associations with plants and other organisms in the soil through a mutualistic relationship where both parties benefit from living together. It implies the antagonistic activities of the rhizobacteria to deter pathogens from invading tomato plants through their roots. Some PGPR are regarded as biological control agents that hinder the development of spoilage organisms and can act as an alternative for agricultural chemicals that may be detrimental to the health of humans, animals, and some of the beneficial microbes in the rhizosphere soil. These bacteria also help tomato plants acquire essential nutrients like potassium (K), magnesium (Mg), phosphorus (P), and nitrogen (N). Some rhizobacteria may offer a solution to low tomato production and help tackle food insecurity and farming problems. In this review, an overview of soil-inhabiting rhizobacteria focused on improving the sustainable production of Solanum lycopersicum ., Competing Interests: The authors declare there are no competing interests., (©2022 Adedayo et al.)
- Published
- 2022
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27. Wheat Metabolite Interferences on Fluorescent Pseudomonas Physiology Modify Wheat Metabolome through an Ecological Feedback.
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Rieusset L, Rey M, Wisniewski-Dyé F, Prigent-Combaret C, and Comte G
- Abstract
Plant roots exude a wide variety of secondary metabolites able to attract and/or control a large diversity of microbial species. In return, among the root microbiota, some bacteria can promote plant development. Among these, Pseudomonas are known to produce a wide diversity of secondary metabolites that could have biological activity on the host plant and other soil microorganisms. We previously showed that wheat can interfere with Pseudomonas secondary metabolism production through its root metabolites. Interestingly, production of Pseudomonas bioactive metabolites, such as phloroglucinol, phenazines, pyrrolnitrin, or acyl homoserine lactones, are modified in the presence of wheat root extracts. A new cross metabolomic approach was then performed to evaluate if wheat metabolic interferences on Pseudomonas secondary metabolites production have consequences on wheat metabolome itself. Two different Pseudomonas strains were conditioned by wheat root extracts from two genotypes, leading to modification of bacterial secondary metabolites production. Bacterial cells were then inoculated on each wheat genotypes. Then, wheat root metabolomes were analyzed by untargeted metabolomic, and metabolites from the Adular genotype were characterized by molecular network. This allows us to evaluate if wheat differently recognizes the bacterial cells that have already been into contact with plants and highlights bioactive metabolites involved in wheat- Pseudomonas interaction.
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- 2022
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28. Effect of Inoculation Level on the Impact of the PGPR Azospirillum lipoferum CRT1 on Selected Microbial Functional Groups in the Rhizosphere of Field Maize.
- Author
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Renoud S, Abrouk D, Prigent-Combaret C, Wisniewski-Dyé F, Legendre L, Moënne-Loccoz Y, and Muller D
- Abstract
The impact of inoculated plant growth-promoting rhizobacteria (PGPR) on its host physiology and nutrition depends on inoculum level. Whether the impact of the inoculated PGPR on the indigenous rhizosphere microbiota also varies with the PGPR inoculum level is unclear. Here, we tested this issue using the PGPR Azospirillum lipoferum CRT1-maize model system, where the initial seed inoculation is known to enhance maize growth and germination, and impacts the maize rhizomicrobiota, including microbial functional groups modulating plant growth. A. lipoferum CRT1 was added to the seeds at standard (10
5-6 cells.seed-1 ) or reduced (104-5 cells.seed-1 ) inoculation levels, in three fields. The effect of the two PGPR formulations was assessed on maize growth and on the nifH (nitrogen fixation), acdS (ACC deaminase activity) and phlD (2,4-diacetylphloroglucinol production) microbial functional groups. The size of the three functional groups was monitored by qPCR at the six-leaf stage and the flowering stage, and the diversity of the nifH and acdS functional groups (as well as the bacterial community) were estimated by MiSeq metabarcoding at the six-leaf stage. The results showed that the benefits of the reduced inoculant formulation were significant in two out of three fields, but different (often lower) than those of the standard formulation. The effects of formulations on the size of the three functional groups differed, and depended on field site and functional group. The reduced formulation had an impact on the diversity of nifH and acdS groups at one site, whereas the standard formulation had an impact at the two other sites. Inoculation significantly impacted the total bacterial community in the three fields, but only with the reduced formulation. In conclusion, the reduced inoculant formulation impacted the indigenous rhizosphere microbiota differently, but not less efficiently, than the standard formulation.- Published
- 2022
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29. Field Site-Specific Effects of an Azospirillum Seed Inoculant on Key Microbial Functional Groups in the Rhizosphere.
- Author
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Renoud S, Vacheron J, Abrouk D, Prigent-Combaret C, Legendre L, Muller D, and Moënne-Loccoz Y
- Abstract
The beneficial effects of plant growth-promoting Rhizobacteria (PGPR) entail several interaction mechanisms with the plant or with other root-associated microorganisms. These microbial functions are carried out by multiple taxa within functional groups and contribute to rhizosphere functioning. It is likely that the inoculation of additional PGPR cells will modify the ecology of these functional groups. We also hypothesized that the inoculation effects on functional groups are site specific, similarly as the PGPR phytostimulation effects themselves. To test this, we assessed in the rhizosphere of field-grown maize the effect of seed inoculation with the phytostimulatory PGPR Azospirillum lipoferum CRT1 on the size and/or diversity of selected microbial functional groups important for plant growth, using quantitative polymerase chain reaction and/or Illumina MiSeq metabarcoding. The functional groups included bacteria able to fix nitrogen (a key nutrient for plant growth), producers of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (which modulate ethylene metabolism in plant and stimulate root growth), and producers of 2,4-diacetylphloroglucinol (an auxinic signal enhancing root branching). To test the hypothesis that such ecological effects were site-specific, the functional groups were monitored at three different field sites, with four sampling times over two consecutive years. Despite poor inoculant survival, inoculation enhanced maize growth. It also increased the size of functional groups in the three field sites, at the maize six-leaf and flowering stages for diazotrophs and only at flowering stage for ACC deaminase and 2,4-diacetylphloroglucinol producers. Sequencing done in the second year revealed that inoculation modified the composition of diazotrophs (and of the total bacterial community) and to a lesser extent of ACC deaminase producers. This study revealed an ecological impact that was field specific (even though a few taxa were impacted in all fields) and of unexpected magnitude with the phytostimulatory Azospirillum inoculant, when considering microbial functional groups. Further methodological developments are needed to monitor additional functional groups important for soil functioning and plant growth under optimal or stress conditions., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Renoud, Vacheron, Abrouk, Prigent-Combaret, Legendre, Muller and Moënne-Loccoz.)
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- 2022
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30. Lipopeptide Interplay Mediates Molecular Interactions between Soil Bacilli and Pseudomonads.
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Andrić S, Meyer T, Rigolet A, Prigent-Combaret C, Höfte M, Balleux G, Steels S, Hoff G, De Mot R, McCann A, De Pauw E, Argüelles Arias A, and Ongena M
- Subjects
- Bacillus growth & development, Microbial Interactions, Secondary Metabolism, Bacillus metabolism, Lipopeptides metabolism, Pseudomonas metabolism, Soil Microbiology
- Abstract
Some Bacillus species, such as B. velezensis, are important members of the plant-associated microbiome, conferring protection against phytopathogens. However, our knowledge about multitrophic interactions determining the ecological fitness of these biocontrol bacteria in the competitive rhizosphere niche is still limited. Here, we investigated molecular mechanisms underlying interactions between B. velezensis and Pseudomonas as a soil-dwelling competitor. Upon their contact-independent in vitro confrontation, a multifaceted macroscopic outcome was observed and characterized by Bacillus growth inhibition, white line formation in the interaction zone, and enhanced motility. We correlated these phenotypes with the production of bioactive secondary metabolites and identified specific lipopeptides as key compounds involved in the interference interaction and motile response. Bacillus mobilizes its lipopeptide surfactin not only to enhance motility but also to act as a chemical trap to reduce the toxicity of lipopeptides formed by Pseudomonas. We demonstrated the relevance of these unsuspected roles of lipopeptides in the context of competitive tomato root colonization by the two bacterial genera. IMPORTANCE Plant-associated Bacillus velezensis and Pseudomonas spp. represent excellent model species as strong producers of bioactive metabolites involved in phytopathogen inhibition and the elicitation of plant immunity. However, the ecological role of these metabolites during microbial interspecies interactions and the way their expression may be modulated under naturally competitive soil conditions has been poorly investigated. Through this work, we report various phenotypic outcomes from the interactions between B. velezensis and 10 Pseudomonas strains used as competitors and correlate them with the production of specific metabolites called lipopeptides from both species. More precisely, Bacillus overproduces surfactin to enhance motility, which also, by acting as a chemical trap, reduces the toxicity of other lipopeptides formed by Pseudomonas. Based on data from interspecies competition on plant roots, we assume this would allow Bacillus to gain fitness and persistence in its natural rhizosphere niche. The discovery of new ecological functions for Bacillus and Pseudomonas secondary metabolites is crucial to rationally design compatible consortia, more efficient than single-species inoculants, to promote plant health and growth by fighting economically important pathogens in sustainable agriculture.
- Published
- 2021
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31. Small-Scale Variability in Bacterial Community Structure in Different Soil Types.
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Hugoni M, Nunan N, Thioulouse J, Dubost A, Abrouk D, Martins JMF, Goffner D, Prigent-Combaret C, and Grundmann G
- Subjects
- Biodiversity, Ecosystem, RNA, Ribosomal, 16S genetics, Soil Microbiology, Microbiota, Soil
- Abstract
Microbial spatial distribution has mostly been studied at field to global scales (i.e., ecosystem scales). However, the spatial organization at small scales (i.e., centimeter to millimeter scales), which can help improve our understanding of the impacts of spatial communities structure on microbial functioning, has received comparatively little attention. Previous work has shown that small-scale spatial structure exists in soil microbial communities, but these studies have not compared soils from geographically distant locations, nor have they utilized community ecology approaches, such as the core and satellite hypothesis and/or abundance-occupancy relationships, often used in macro-ecology, to improve the description of the spatial organization of communities. In the present work, we focused on bacterial diversity (i.e., 16S rRNA gene sequencing) occurring in micro-samples from a variety of locations with different pedo-climatic histories (i.e., from semi-arid, alpine, and temperate climates) and physicochemical properties. The forms of ecological spatial relationships in bacterial communities (i.e., occupancy-frequency and abundance-occupancy) and taxa distributions (i.e., habitat generalists and specialists) were investigated. The results showed that bacterial composition differed in the four soils at the small scale. Moreover, one soil presented a satellite mode distribution, whereas the three others presented bimodal distributions. Interestingly, numerous core taxa were present in the four soils among which 8 OTUs were common to the four sites. These results confirm that analyses of the small-scale spatial distribution are necessary to understand consequent functional processes taking place in soils, affecting thus ecosystem functioning., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.)
- Published
- 2021
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32. Experimental Evolution in Plant-Microbe Systems: A Tool for Deciphering the Functioning and Evolution of Plant-Associated Microbial Communities.
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Manriquez B, Muller D, and Prigent-Combaret C
- Abstract
In natural environments, microbial communities must constantly adapt to stressful environmental conditions. The genetic and phenotypic mechanisms underlying the adaptive response of microbial communities to new (and often complex) environments can be tackled with a combination of experimental evolution and next generation sequencing. This combination allows to analyse the real-time evolution of microbial populations in response to imposed environmental factors or during the interaction with a host, by screening for phenotypic and genotypic changes over a multitude of identical experimental cycles. Experimental evolution (EE) coupled with comparative genomics has indeed facilitated the monitoring of bacterial genetic evolution and the understanding of adaptive evolution processes. Basically, EE studies had long been done on single strains, allowing to reveal the dynamics and genetic targets of natural selection and to uncover the correlation between genetic and phenotypic adaptive changes. However, species are always evolving in relation with other species and have to adapt not only to the environment itself but also to the biotic environment dynamically shaped by the other species. Nowadays, there is a growing interest to apply EE on microbial communities evolving under natural environments. In this paper, we provide a non-exhaustive review of microbial EE studies done with systems of increasing complexity (from single species, to synthetic communities and natural communities) and with a particular focus on studies between plants and plant-associated microorganisms. We highlight some of the mechanisms controlling the functioning of microbial species and their adaptive responses to environment changes and emphasize the importance of considering bacterial communities and complex environments in EE studies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Manriquez, Muller and Prigent-Combaret.)
- Published
- 2021
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33. A Cross-Metabolomic Approach Shows that Wheat Interferes with Fluorescent Pseudomonas Physiology through Its Root Metabolites.
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Rieusset L, Rey M, Gerin F, Wisniewski-Dyé F, Prigent-Combaret C, and Comte G
- Abstract
Roots contain a wide variety of secondary metabolites. Some of them are exudated in the rhizosphere, where they are able to attract and/or control a large diversity of microbial species. In return, the rhizomicrobiota can promote plant health and development. Some rhizobacteria belonging to the Pseudomonas genus are known to produce a wide diversity of secondary metabolites that can exert a biological activity on the host plant and on other soil microorganisms. Nevertheless, the impact of the host plant on the production of bioactive metabolites by Pseudomonas is still poorly understood. To characterize the impact of plants on the secondary metabolism of Pseudomonas , a cross-metabolomic approach has been developed. Five different fluorescent Pseudomonas strains were thus cultivated in the presence of a low concentration of wheat root extracts recovered from three wheat genotypes. Analysis of our metabolomic workflow revealed that the production of several Pseudomonas secondary metabolites was significantly modulated when bacteria were cultivated with root extracts, including metabolites involved in plant-beneficial properties.
- Published
- 2021
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34. Secondary metabolites from plant-associated Pseudomonas are overproduced in biofilm.
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Rieusset L, Rey M, Muller D, Vacheron J, Gerin F, Dubost A, Comte G, and Prigent-Combaret C
- Subjects
- Acyl-Butyrolactones, Bacteria, Rhizosphere, Biofilms, Pseudomonas genetics
- Abstract
Plant rhizosphere soil houses complex microbial communities in which microorganisms are often involved in intraspecies as well as interspecies and inter-kingdom signalling networks. Some members of these networks can improve plant health thanks to an important diversity of bioactive secondary metabolites. In this competitive environment, the ability to form biofilms may provide major advantages to microorganisms. With the aim of highlighting the impact of bacterial lifestyle on secondary metabolites production, we performed a metabolomic analysis on four fluorescent Pseudomonas strains cultivated in planktonic and biofilm colony conditions. The untargeted metabolomic analysis led to the detection of hundreds of secondary metabolites in culture extracts. Comparison between biofilm and planktonic conditions showed that bacterial lifestyle is a key factor influencing Pseudomonas metabolome. More than 50% of the detected metabolites were differentially produced according to planktonic or biofilm lifestyles, with the four Pseudomonas strains overproducing several secondary metabolites in biofilm conditions. In parallel, metabolomic analysis associated with genomic prediction and a molecular networking approach enabled us to evaluate the impact of bacterial lifestyle on chemically identified secondary metabolites, more precisely involved in microbial interactions and plant-growth promotion. Notably, this work highlights the major effect of biofilm lifestyle on acyl-homoserine lactone and phenazine production in P. chlororaphis strains., (© 2020 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.)
- Published
- 2020
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35. Vaginal Tampon Colonization by Staphylococcus aureus in Healthy Women.
- Author
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Chiaruzzi M, Barbry A, Muggeo A, Tristan A, Jacquemond I, Badiou C, Cluzeau L, Bourdeau S, Durand T, Engelmann A, Bosquet D, Bes M, Prigent-Combaret C, Thioulouse J, Muller D, and Lina G
- Subjects
- Adolescent, Adult, Bacterial Toxins analysis, Female, France epidemiology, Humans, Middle Aged, Prevalence, Staphylococcal Infections microbiology, Staphylococcus aureus chemistry, Staphylococcus aureus genetics, Young Adult, Menstrual Hygiene Products microbiology, Staphylococcal Infections epidemiology, Staphylococcus aureus isolation & purification
- Abstract
Tampons recovered from a cohort of 737 healthy women (median age, 32 years) were analyzed for the presence of Staphylococcus aureus A total of 198 tampons (27%) were colonized by S. aureus , 28 (4%) by a strain producing toxic shock syndrome toxin 1 (TSST-1). S. aureus was detected more frequently in tampons that did not require an applicator for their insertion (74/233 [32%] versus 90/381 [24%]; odds ratio [OR] = 1.51 [95% confidence interval, 1.04 to 2.17]) and in women who used an intrauterine device for contraception (53/155 [34%] versus 145/572 [27%]; OR = 1.53 [95% confidence interval, 1.05 to 2.24]). The S. aureus strains isolated from tampons belonged to 22 different clonal complexes (CCs). The most prevalent CC was CC398 agr 1 ( n = 57 [27%]), a clone that does not produce superantigenic toxins, followed by CC30 agr 3 ( n = 27, 13%), producing TSST-1 (24/27 [89%]), the principal clone of S. aureus involved in menstrual toxic shock syndrome (MTSS). IMPORTANCE Menstrual toxic shock syndrome (MTSS) is an uncommon severe acute disease that occurs in healthy menstruating women colonized by TSST-1-producing S. aureus who use intravaginal protection, such as tampons and menstrual cups. The catamenial product collected by the protection serves as a growth medium for S. aureus and allows TSST-1 production. Previous studies evaluated the prevalence of genital colonization by S. aureus by vaginal swabbing, but they did not examine tampon colonization. This study demonstrated a high prevalence of tampon colonization by S. aureus and the presence of the CC30 TSST-1 S. aureus clone responsible for MTSS in tampons from healthy women. The results support the vaginal carriage of this lineage in healthy women. In addition, the higher prevalence of S. aureus within tampons that do not require an applicator indicates a crucial role for handwashing before tampon handling to decrease the risk of tampon contamination., (Copyright © 2020 American Society for Microbiology.)
- Published
- 2020
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36. Co-occurrence of rhizobacteria with nitrogen fixation and/or 1-aminocyclopropane-1-carboxylate deamination abilities in the maize rhizosphere.
- Author
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Renoud S, Bouffaud ML, Dubost A, Prigent-Combaret C, Legendre L, Moënne-Loccoz Y, and Muller D
- Subjects
- Deamination, Nitrogen Fixation, Soil Microbiology, Rhizosphere, Zea mays
- Abstract
The plant microbiota may differ depending on soil type, but these microbiota probably share the same functions necessary for holobiont fitness. Thus, we tested the hypothesis that phytostimulatory microbial functional groups are likely to co-occur in the rhizosphere, using groups corresponding to nitrogen fixation (nifH) and 1-aminocyclopropane-1-carboxylate deamination (acdS), i.e. two key modes of action in plant-beneficial rhizobacteria. The analysis of three maize fields in two consecutive years showed that quantitative PCR numbers of nifH and of acdS alleles differed according to field site, but a positive correlation was found overall when comparing nifH and acdS numbers. Metabarcoding analyses in the second year indicated that the diversity level of acdS but not nifH rhizobacteria in the rhizosphere differed across fields. Furthermore, between-class analysis showed that the three sites differed from one another based on nifH or acdS sequence data (or rrs data), and the bacterial genera contributing most to field differentiation were not the same for the three bacterial groups. However, co-inertia analysis indicated that the genetic structures of both functional groups and of the whole bacterial community were similar across the three fields. Therefore, results point to co-selection of rhizobacteria harboring nitrogen fixation and/or 1-aminocyclopropane-1-carboxylate deamination abilities., (© FEMS 2020.)
- Published
- 2020
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37. Author Correction: Complex ecological interactions of Staphylococcus aureus in tampons during menstruation.
- Author
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Jacquemond I, Muggeo A, Lamblin G, Tristan A, Gillet Y, Bolze PA, Bes M, Gustave CA, Rasigade JP, Golfier F, Ferry T, Dubost A, Abrouk D, Barreto S, Prigent-Combaret C, Thioulouse J, Lina G, and Muller D
- Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2020
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38. Ancient wheat varieties have a higher ability to interact with plant growth-promoting rhizobacteria.
- Author
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Valente J, Gerin F, Le Gouis J, Moënne-Loccoz Y, and Prigent-Combaret C
- Subjects
- Genotype, Nuclear Proteins metabolism, Plant Proteins metabolism, Plant Roots metabolism, Poaceae, Pseudomonas metabolism, Soil, Soil Microbiology, Triticum classification, Triticum metabolism, Plant Roots microbiology, Rhizobiaceae physiology, Triticum growth & development
- Abstract
Plant interactions with plant growth-promoting rhizobacteria (PGPR) are highly dependent on plant genotype. Modern plant breeding has largely sought to improve crop performance but with little focus on the optimization of plant × PGPR interactions. The interactions of the model PGPR strain Pseudomonas kilonensis F113 were therefore compared in 199 ancient and modern wheat genotypes. A reporter system, in which F113 colonization and expression of 2,4-diacetylphloroglucinol biosynthetic genes (phl) were measured on roots was used to quantify F113 × wheat interactions under gnotobiotic conditions. Thereafter, eight wheat accessions that differed in their ability to interact with F113 were inoculated with F113 and grown in greenhouse in the absence or presence of stress. F113 colonization was linked to improved stress tolerance. Moreover, F113 colonization and phl expression were higher overall on ancient genotypes than modern genotypes. F113 colonization improved wheat performance in the four genotypes that showed the highest level of phl expression compared with the four genotypes in which phl expression was lowest. Taken together, these data suggest that recent wheat breeding strategies have had a negative impact on the ability of the plants to interact with PGPR., (© 2019 John Wiley & Sons Ltd.)
- Published
- 2020
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39. Does in vitro selection of biocontrol agents guarantee success in planta? A study case of wheat protection against Fusarium seedling blight by soil bacteria.
- Author
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Besset-Manzoni Y, Joly P, Brutel A, Gerin F, Soudière O, Langin T, and Prigent-Combaret C
- Subjects
- France, Rhizosphere, Seedlings, Soil Microbiology, Bacteria growth & development, Bacteria isolation & purification, Biological Control Agents, Fusarium physiology, Plant Diseases microbiology, Plant Diseases prevention & control, Plant Roots microbiology, Triticum microbiology
- Abstract
Biological control is a great hope for reducing the overutilization of pesticides in agricultural soils. It often involves microorganisms or molecules produced by microorganisms that will be able to interact with either a plant or pathogens of this plant to reduce the growth of the pathogen and limit its negative impact on the host plant. When new biocontrol products are developed, strains were mostly selected based on their ability to inhibit a pathogen of interest under in vitro conditions via antagonistic effects. Strains with no in vitro effect are often discarded and not tested in planta. But is the in vitro selection of bacterial agents according to their antagonism activities towards a plant pathogen the best way to get effective biocontrol products? To answer this question, we used wheat and the fungal pathogen Fusarium graminearum as a study pathosystem model. A library of 205 soil bacteria was screened in 2 types of in vitro growth inhibition tests against F. graminearum, and in an in planta experiment. We find strains which do not have inhibition phenotypes in vitro but good efficacy in planta. Interestingly, some strains belong to species (Microbacterium, Arthrobacter, Variovorax) that are not known in the literature for their ability to protect plants against fungal pathogens. Thus, developing a biocontrol product against F. graminearum must be preferentially based on the direct screening of strains for their protective activity on wheat plants against fungal diseases, rather than on their in vitro antagonistic effects on fungal growth., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
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40. Draft Genome Sequence of Bacillus velezensis Strain ZeaDK315Endo16.
- Author
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Aremu BR, Prigent-Combaret C, and Babalola OO
- Abstract
Here, we report the draft genome sequence of the endophytic Bacillus velezensis strain ZeaDK315Endo16, isolated from DK315 maize from Lyon, France. B. velezensis ZeaDK315Endo16 exhibits a suppressive ability toward Fusarium graminearum , a widely known threat to maize production and quality., (Copyright © 2019 Aremu et al.)
- Published
- 2019
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41. Genomic, phylogenetic and catabolic re-assessment of the Pseudomonas putida clade supports the delineation of Pseudomonas alloputida sp. nov., Pseudomonas inefficax sp. nov., Pseudomonas persica sp. nov., and Pseudomonas shirazica sp. nov.
- Author
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Keshavarz-Tohid V, Vacheron J, Dubost A, Prigent-Combaret C, Taheri P, Tarighi S, Taghavi SM, Moënne-Loccoz Y, and Muller D
- Subjects
- DNA, Bacterial genetics, Genes, Bacterial genetics, Genes, Essential genetics, Nucleic Acid Hybridization, Phenotype, Pseudomonas putida genetics, Sequence Analysis, DNA, Soil Microbiology, Species Specificity, Genome, Bacterial genetics, Phylogeny, Pseudomonas putida classification
- Abstract
Bacteria of the Pseudomonas putida group are studied for a large panel of properties ranging from plant growth promotion and bioremediation to pathogenicity. To date, most of the classification of individual pseudomonads from this group relies on 16S RNA gene analysis, which is insufficient for accurate taxonomic characterization within bacterial species complexes of the Pseudomonas putida group. Here, a collection of 20 of these bacteria, isolated from various soils, was assessed via multi-locus sequence analysis of rpoD, gyrB and rrs genes. The 20 strains clustered in 7 different clades of the P. putida group. One strain per cluster was sequenced and results were compared to complete genome sequences of type strains of the P. putida group. Phylogenetic analyses, average nucleotide identity data and digital DNA hybridizations, combined to phenotypic characteristics, resulted in the proposition and description of four new species i.e. Pseudomonas alloputida Kh7
T (= LMG 29756T = CFBP 8484T ) sp. nov., Pseudomonas inefficax JV551A3T (= DSM108619T = CFBP 8493T ) sp. nov., Pseudomonas persica RUB6T (= LMG 29757T = CFBP 8486T ) sp. nov. and Pseudomonas shirazica VM14T (= LMG 29953T = CFBP 8487T ) sp. nov., (Copyright © 2019 Elsevier GmbH. All rights reserved.)- Published
- 2019
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42. Draft Genome Sequence of Plant Growth-Promoting Bacillus altitudinis Strain PAE4.
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Ibrahim NAGAA, Omar MNAM, El Heba GAA, Moënne-Loccoz Y, Prigent-Combaret C, and Muller D
- Abstract
We report here the draft genome of Bacillus altitudinis strain PAE4, a thermophilic plant growth-promoting rhizobacterium isolated from the coastal ridge of the Mediterranean Sea in Egypt. Besides heat shock protein genes, several genes encoding phytobeneficial properties were identified.
- Published
- 2018
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43. Chemistry, activity, and impact of plant biocontrol products.
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Bertrand C, Prigent-Combaret C, and Gonzales-Coloma A
- Published
- 2018
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44. Exploiting rhizosphere microbial cooperation for developing sustainable agriculture strategies.
- Author
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Besset-Manzoni Y, Rieusset L, Joly P, Comte G, and Prigent-Combaret C
- Subjects
- Agriculture, Bacteria classification, Bacteria genetics, Bacteria metabolism, Crops, Agricultural growth & development, Microbiota, Rhizosphere, Bacteria isolation & purification, Crops, Agricultural microbiology, Soil Microbiology
- Abstract
The rhizosphere hosts a considerable microbial community. Among that community, bacteria called plant growth-promoting rhizobacteria (PGPR) can promote plant growth and defense against diseases using diverse distinct plant-beneficial functions. Crop inoculation with PGPR could allow to reduce the use of pesticides and fertilizers in agrosystems. However, microbial crop protection and growth stimulation would be more efficient if cooperation between rhizosphere bacterial populations was taken into account when developing biocontrol agents and biostimulants. Rhizospheric bacteria live in multi-species biofilms formed all along the root surface or sometimes inside the plants (i.e., endophyte). PGPR cooperate with their host plants and also with other microbial populations inside biofilms. These interactions are mediated by a large diversity of microbial metabolites and physical signals that trigger cell-cell communication and appropriate responses. A better understanding of bacterial behavior and microbial cooperation in the rhizosphere could allow for a more successful use of bacteria in sustainable agriculture. This review presents an ecological view of microbial cooperation in agrosystems and lays the emphasis on the main microbial metabolites involved in microbial cooperation, plant health protection, and plant growth stimulation. Eco-friendly inoculant consortia that will foster microbe-microbe and microbe-plant cooperation can be developed to promote crop growth and restore biodiversity and functions lost in agrosystems.
- Published
- 2018
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45. Regulation of Hydroxycinnamic Acid Degradation Drives Agrobacterium fabrum Lifestyles.
- Author
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Meyer T, Renoud S, Vigouroux A, Miomandre A, Gaillard V, Kerzaon I, Prigent-Combaret C, Comte G, Moréra S, Vial L, and Lavire C
- Subjects
- Agrobacterium genetics, Bacterial Proteins, Coumaric Acids chemistry, DNA, Extinction, Biological, Gene Deletion, Gene Expression Regulation, Bacterial, Molecular Structure, Protein Binding, Agrobacterium physiology, Coumaric Acids metabolism
- Abstract
Regulatory factors are key components for the transition between different lifestyles to ensure rapid and appropriate gene expression upon perceiving environmental cues. Agrobacterium fabrum C58 (formerly called A. tumefaciens C58) has two contrasting lifestyles: it can interact with plants as either a rhizosphere inhabitant (rhizospheric lifestyle) or a pathogen that creates its own ecological niche in a plant tumor via its tumor-inducing plasmid (pathogenic lifestyle). Hydroxycinnamic acids are known to play an important role in the pathogenic lifestyle of Agrobacterium spp. but can be degraded in A. fabrum species. We investigated the molecular and ecological mechanisms involved in the regulation of A. fabrum species-specific genes responsible for hydroxycinnamic acid degradation. We characterized the effectors (feruloyl-CoA and p-coumaroyl-CoA) and the DNA targets of the MarR transcriptional repressor, which we named HcaR, which regulates hydroxycinnamic acid degradation. Using an hcaR-deleted strain, we further revealed that hydroxycinnamic acid degradation interfere with virulence gene expression. The HcaR deletion mutant shows a contrasting competitive colonization ability, being less abundant than the wild-type strain in tumors but more abundant in the rhizosphere. This supports the view that A. fabrum C58 HcaR regulation through ferulic and p-coumaric acid perception is important for the transition between lifestyles.
- Published
- 2018
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46. Complex ecological interactions of Staphylococcus aureus in tampons during menstruation.
- Author
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Jacquemond I, Muggeo A, Lamblin G, Tristan A, Gillet Y, Bolze PA, Bes M, Gustave CA, Rasigade JP, Golfier F, Ferry T, Dubost A, Abrouk D, Barreto S, Prigent-Combaret C, Thioulouse J, Lina G, and Muller D
- Subjects
- Adult, DNA Barcoding, Taxonomic, Female, Humans, Menstrual Hygiene Products microbiology, RNA, Ribosomal, 16S, Staphylococcus aureus, Young Adult, Bacteria isolation & purification, Menstruation, Microbiota, Shock, Septic microbiology, Staphylococcal Infections microbiology, Vagina microbiology
- Abstract
Menstrual toxic shock syndrome (mTSS) is a severe disease that occurs in healthy women vaginally colonized by Staphylococcus aureus producing toxic shock toxin 1 and who use tampons. The aim of the present study was to determine the impact of the composition of vaginal microbial communities on tampon colonisation by S. aureus during menses. We analysed the microbiota in menstrual fluids extracted from tampons from 108 healthy women and 7 mTSS cases. Using culture, S. aureus was detected in menstrual fluids of 40% of healthy volunteers and 100% of mTSS patients. Between class analysis of culturomic and 16S rRNA gene metabarcoding data indicated that the composition of the tampons' microbiota differs according to the presence or absence of S. aureus and identify discriminating genera. However, the bacterial communities of tampon fluid positive for S. aureus did not cluster together. No difference in tampon microbiome richness, diversity, and ecological distance was observed between tampon vaginal fluids with or without S. aureus, and between healthy donors carrying S. aureus and mTSS patients. Our results show that the vagina is a major niche of. S. aureus in tampon users and the composition of the tampon microbiota control its virulence though more complex interactions than simple inhibition by lactic acid-producing bacterial species.
- Published
- 2018
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47. Impact of Currently Marketed Tampons and Menstrual Cups on Staphylococcus aureus Growth and Toxic Shock Syndrome Toxin 1 Production In Vitro .
- Author
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Nonfoux L, Chiaruzzi M, Badiou C, Baude J, Tristan A, Thioulouse J, Muller D, Prigent-Combaret C, and Lina G
- Subjects
- Bacterial Toxins analysis, Biofilms, Cotton Fiber analysis, Cotton Fiber microbiology, Culture Media, Enterotoxins analysis, Female, Humans, Oxygen metabolism, Shock, Septic microbiology, Shock, Septic prevention & control, Staphylococcal Infections complications, Superantigens analysis, Vagina microbiology, Bacterial Toxins biosynthesis, Enterotoxins biosynthesis, Menstrual Hygiene Products microbiology, Staphylococcus aureus growth & development, Staphylococcus aureus metabolism, Superantigens biosynthesis
- Abstract
Fifteen currently marketed intravaginal protection products (11 types of tampon and 4 types of menstrual cup) were tested by the modified tampon sac method to determine their effect on Staphylococcus aureus growth and toxic shock syndrome toxin 1 (TSST-1) production. Most tampons reduced S. aureus growth and TSST-1 production, with differences based on brand and composition, and the level of S. aureus growth was higher in destructured than in unaltered tampons. We observed higher levels of S. aureus growth and toxin production in menstrual cups than in tampons, potentially due to the additional air introduced into the bag by cups, with differences based on cup composition and size. IMPORTANCE Menstrual toxic shock syndrome is a rare but severe disease. It occurs in healthy women vaginally colonized by Staphylococcus aureus producing toxic shock syndrome toxin 1 using intravaginal protection, such as tampons or menstrual cups. Intravaginal protection induces TSS by the collection of catamenial products, which act as a growth medium for S. aureus Previous studies evaluated the impact of tampon composition on S. aureus producing toxic shock syndrome toxin 1, but they are not recent and did not include menstrual cups. This study demonstrates that highly reproducible results for S. aureus growth and TSST-1 production can be obtained by using a simple protocol that reproduces the physiological conditions of tampon and cup usage as closely as possible, providing recommendations for tampon or cup use to both manufacturers and consumers. Notably, our results do not show that menstrual cups are safer than tampons and suggest that they require similar precautions., (Copyright © 2018 American Society for Microbiology.)
- Published
- 2018
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48. Differential Contribution of Plant-Beneficial Functions from Pseudomonas kilonensis F113 to Root System Architecture Alterations in Arabidopsis thaliana and Zea mays.
- Author
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Vacheron J, Desbrosses G, Renoud S, Padilla R, Walker V, Muller D, and Prigent-Combaret C
- Subjects
- Bacterial Proteins genetics, Bacterial Proteins metabolism, Gene Deletion, Gene Expression Regulation, Bacterial physiology, Pseudomonas genetics, Symbiosis, Arabidopsis physiology, Plant Roots growth & development, Plant Roots microbiology, Pseudomonas physiology, Zea mays physiology
- Abstract
Fluorescent pseudomonads are playing key roles in plant-bacteria symbiotic interactions due to the multiple plant-beneficial functions (PBFs) they are harboring. The relative contributions of PBFs to plant-stimulatory effects of the well-known plant growth-promoting rhizobacteria Pseudomonas kilonensis F113 (formerly P. fluorescens F113) were investigated using a genetic approach. To this end, several deletion mutants were constructed, simple mutants ΔphlD (impaired in the biosynthesis of 2,4-diacetylphloroglucinol [DAPG]), ΔacdS (deficient in 1-aminocyclopropane-1-carboxylate deaminase activity), Δgcd (glucose dehydrogenase deficient, impaired in phosphate solubilization), and ΔnirS (nitrite reductase deficient), and a quadruple mutant (deficient in the four PBFs mentioned above). Every PBF activity was quantified in the wild-type strain and the five deletion mutants. This approach revealed few functional interactions between PBFs in vitro. In particular, biosynthesis of glucose dehydrogenase severely reduced the production of DAPG. Contrariwise, the DAPG production impacted positively, but to a lesser extent, phosphate solubilization. Inoculation of the F113 wild-type strain on Arabidopsis thaliana Col-0 and maize seedlings modified the root architecture of both plants. Mutant strain inoculations revealed that the relative contribution of each PBF differed according to the measured plant traits and that F113 plant-stimulatory effects did not correspond to the sum of each PBF relative contribution. Indeed, two PBF genes (ΔacdS and ΔnirS) had a significant impact on root-system architecture from both model plants, in in vitro and in vivo conditions. The current work underscored that few F113 PBFs seem to interact between each other in the free-living bacterial cells, whereas they control in concert Arabidopsis thaliana and maize growth and development.
- Published
- 2018
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49. Phylogenetic diversity and antagonistic traits of root and rhizosphere pseudomonads of bean from Iran for controlling Rhizoctonia solani.
- Author
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Keshavarz-Tohid V, Taheri P, Muller D, Prigent-Combaret C, Vacheron J, Taghavi SM, Tarighi S, and Moënne-Loccoz Y
- Subjects
- Iran, Plant Roots microbiology, Pseudomonas classification, Pseudomonas genetics, Rhizosphere, Soil Microbiology, Antibiosis, Fabaceae microbiology, Phylogeny, Plant Diseases microbiology, Pseudomonas isolation & purification, Pseudomonas physiology, Rhizoctonia physiology
- Abstract
Fluorescent pseudomonads from bean root and rhizosphere in Iran were investigated for biocontrol of the fungal pathogen Rhizoctonia solani. Phylogenetic analysis of concatenated 16S rRNA, gyrB and rpoD sequences for 33 Pseudomonas isolates showed that 15 belonged to four clusters within the 'P. fluorescens' group, i.e. one corresponding to P. thivervalensis, two others including P. moraviensis or P. baetica, and the last one without closely-related established species. The 18 other isolates belonged to five clusters within the 'P. putida' group, one including P. mosselii and P. entomophila, another including strains currently described as P. putida, and three without closely-related species described. Ten isolates were selected based on in vitro inhibition of R. solani. Cellulase activity was identified in three pseudomonads, chitinase activity in two pseudomonads, extracellular protease activity in nine pseudomonads and hydrogen cyanide production in two pseudomonads. Genes coding for production of phenazine, pyoluteorin, pyrrolnitrin and 2,4-diacetylphloroglucinol were not found, whereas the 1-aminocyclopropane-1-carboxylate deamination gene acdS was present in three pseudomonads. The antagonistic acdS
+ strain VKh13 from the 'P. putida' group effectively protected soil-grown bean from R. solani AG 4-HGI. Results show that pseudomonads from uncharacterized taxa were readily obtained from Iranian soils and displayed biocontrol potential against R. solani., (Copyright © 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)- Published
- 2017
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50. Distribution of 2,4-Diacetylphloroglucinol Biosynthetic Genes among the Pseudomonas spp. Reveals Unexpected Polyphyletism.
- Author
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Almario J, Bruto M, Vacheron J, Prigent-Combaret C, Moënne-Loccoz Y, and Muller D
- Abstract
Fluorescent pseudomonads protecting plant roots from phytopathogens by producing 2,4-diacetylphloroglucinol (DAPG) are considered to form a monophyletic lineage comprised of DAPG
+ Pseudomonas strains in the " P. corrugata " and " P. protegens " subgroups of the " Pseudomonas fluorescens " group. However, DAPG production ability has not been investigated for many species of these two subgroups, and whether or not the DAPG+ Pseudomonas are truly monophyletic remained to be verified. Thus, the distribution of the DAPG biosynthetic operon ( phlACBD genes) in the Pseudomonas spp. was investigated in sequenced genomes and type strains. Results showed that the DAPG+ Pseudomonas include species of the " P. fluorescens " group, i.e., P. protegens, P. brassicacearum, P. kilonensis , and P. thivervalensis , as expected, as well as P. gingeri in which it had not been documented. Surprisingly, they also include bacteria outside the " P. fluorescens" group, as exemplified by Pseudomonas sp. OT69, and even two Betaproteobacteria genera. The phl operon-based phylogenetic tree was substantially congruent with the one inferred from concatenated housekeeping genes rpoB, gyrB , and rrs . Contrariwise to current supposition, ancestral character reconstructions favored multiple independent acquisitions rather that one ancestral event followed by vertical inheritance. Indeed, based on synteny analyses, these acquisitions appeared to vary according to the Pseudomonas subgroup and even the phylogenetic groups within the subgroups. In conclusion, our study shows that the phl+ Pseudomonas populations form a polyphyletic group and suggests that DAPG biosynthesis might not be restricted to this genus. This is important to consider when assessing the ecological significance of phl+ bacterial populations in rhizosphere ecosystems.- Published
- 2017
- Full Text
- View/download PDF
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