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191 results on '"Preziuso, Silvia"'

7. Effect of a Probiotic Mixture in Captive Cheetahs (Acinonyx Jubatus) with Gastrointestinal Symptoms—A Pilot Study

Author

Mangiaterra, Sara, primary, Schmidt-Küntzel, Anne, additional, Marker, Laurie, additional, Di Cerbo, Alessandro, additional, Piccinini, Renato, additional, Guadagnini, Davide, additional, Turba, Maria Elena, additional, Berardi, Sara, additional, Galosi, Livio, additional, Preziuso, Silvia, additional, Cerquetella, Matteo, additional, and Rossi, Giacomo, additional

Published
2022
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8. Globetrotting strangles: the unbridled national and international transmission of Streptococcus equi between horses

Author

Wilson, Hayley, Mitchell, Catriona, Steward, Karen, Charbonneau, Amelia, Walsh, Saoirse, Timoney, John, Wernery, Ulli, Joseph, Marina, Craig, David, Van Maanen, Kees, Hoogkamer-van Gennep, Annelies, Léon, Albertine, Witkowski, Lucjan, Rzewuska, Magdalena, Stefańska, Ilona, van Loon, Gunther, Cursons, Ray, Patty, Olivia, Acke, Els, Gilkerson, James, El-Hage, Charles, Allen, Joanne, Bannai, Hiroshi, Kinoshita, Yuta, Niwa, Hidekazu, Becú, Teótimo, Pringle, John, Guss, Bengt, Böse, Reinhard, Abbott, Yvonne, Katz, Lisa, Leggett, Bernadette, Buckley, Tom, Blum, Shlomo, Cruz López, Fátima, Fernández Ros, Ana, Marotti Campi, Maria Cristina, Preziuso, Silvia, Robinson, Carl, Newton, J. Richard, Schofield, Ellen, Brooke, Ben, Boursnell, Mike, de Brauwere, Nicolas, Kirton, Roxane, Barton, Charlotte, Abudahab, Khalil, Taylor, Ben, Yeats, Corin, Goater, Richard, Aanensen, David, Harris, Simon, Parkhill, Julian, Holden, Matthew, Waller, Andrew, Animal Health Trust (AHT), University of Cambridge [UK] (CAM), Technology Networks, Xampla, Free University of Berlin (FU), Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Central Veterinary Research Laboratory, Emirates Racing Authority, Animal Health Service (GD), LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO), Unité de Recherche Risques Microbiens (U2RM), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Universiteit Gent = Ghent University [Belgium] (UGENT), University of Waikato [Hamilton], Massey University, University of Melbourne, Japan Racing Association, Clinica Equina Buenos Aires, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), Labor Dr Böse GmbH, University College Dublin [Dublin] (UCD), Irish Equine Centre (IEC), Kimron Veterinary Institute (KVI), Facultad de Veterinaria, Universidad Complutense, Universidad Complutense de Madrid = Complutense University of Madrid [Madrid] (UCM), Exopol Zaragoza, Al Khalediah Equine Hospital, University of Camerino, Redwings Horse Sanctuary, Royal Society for the Prevention of Cruelty to Animals, Weatherford Equine Medical Centre, Centre for Genomic Pathogen Surveillance, The Wellcome Trust Sanger Institute [Cambridge], Nuffield Department of Medicine [Oxford, UK] (Big Data Institute), University of Oxford [Oxford], Microbiotica Limited, University of St Andrews [Scotland], and Intervacc AB

Subjects
0403 veterinary science, genome diversity, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, 040301 veterinary sciences, pandemic, 0402 animal and dairy science, transmission, Streptococcus equi, 04 agricultural and veterinary sciences, General Medicine, strangles, 040201 dairy & animal science
Abstract

International audience; Background: Streptococcus equi subspecies equi (S.equi) is the cause of the highly contagious equine respiratory disease ‘strangles’. Approximately 10% of recovered animals can persistently carry the bacteria and transmit it to naïve animals. The global movement of horses is an ideal mechanism for widespread transmission to geographically distant locations.Objectives: Utilise whole-genome sequence data to disentangle the transmission of S. equi and subsequent outbreaks of strangles.Study design: In vitro analysis of micro-organisms.Methods: Isolates (n = 670) of S. equi were recovered from clinical samples submitted to multiple collaborating clinics and institutions globally. Following species confirmation, isolates underwent whole-genome sequencing using Illumina technology. Sequence reads passing quality control measures were assembled and uploaded to Pathogenwatch, which assigned a phylogeny based upon sequences of core genome alleles. Population structure was inferred using the population mixture analysis in BAPS.Results: BAPS clustered the isolates into six different clusters (BAPS 1-6) and showed dominant lineages in different geographical areas but also global transmission within the clusters. Sub-groups within the clusters highlighted multiple outbreaks at local, national and international scales and highlighted population structures and transmission dynamics within single locations. For example, four different strains collected over just seven months were identified in a single location. Sequence data also identified a statistically significant decline in BAPS-5 since 2010.Main limitations: Pathogenwatch has shown its utility in investigating S. equi transmission and population structure. However, it is based upon a curated set of 1286 core genome loci. Further investigations will need to be conducted using the full spectrum of data available from whole-genome sequencing.Conclusions: Pathogenwatch was used as a tool to rapidly identify and visualise the whole-genome sequence data of a large S. equi dataset. The data demonstrate widespread transmission of multiple S. equi lineages and provide strong evidence that asymptomatic carrier horses are perpetuating this dissemination.

Published
2021

10. Globetrotting strangles: the unbridled national and international transmission of Streptococcus equi between horses

Author

Mitchell, Catriona, primary, Steward, Karen F., additional, Charbonneau, Amelia R. L., additional, Walsh, Saoirse, additional, Wilson, Hayley, additional, Timoney, John F., additional, Wernery, Ulli, additional, Joseph, Marina, additional, Craig, David, additional, van Maanen, Kees, additional, Hoogkamer-van Gennep, Annelies, additional, Leon, Albertine, additional, Witkowski, Lucjan, additional, Rzewuska, Magdalena, additional, Stefańska, Ilona, additional, Żychska, Monika, additional, van Loon, Gunther, additional, Cursons, Ray, additional, Patty, Olivia, additional, Acke, Els, additional, Gilkerson, James R., additional, El-Hage, Charles, additional, Allen, Joanne, additional, Bannai, Hiroshi, additional, Kinoshita, Yuta, additional, Niwa, Hidekazu, additional, Becú, Teótimo, additional, Pringle, John, additional, Guss, Bengt, additional, Böse, Reinhard, additional, Abbott, Yvonne, additional, Katz, Lisa, additional, Leggett, Bernadette, additional, Buckley, Tom C., additional, Blum, Shlomo E., additional, Cruz López, Fátima, additional, Fernández Ros, Ana, additional, Marotti Campi, Maria Cristina, additional, Preziuso, Silvia, additional, Robinson, Carl, additional, Newton, J. Richard, additional, Schofield, Ellen, additional, Brooke, Ben, additional, Boursnell, Mike, additional, de Brauwere, Nicolas, additional, Kirton, Roxane, additional, Barton, Charlotte K., additional, Abudahab, Khalil, additional, Taylor, Ben, additional, Yeats, Corin A., additional, Goater, Richard, additional, Aanensen, David M., additional, Harris, Simon R., additional, Parkhill, Julian, additional, Holden, Matthew T. G., additional, and Waller, Andrew S., additional

Published
2021
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13. Ovine Paratuberculosis: A Seroprevalence Study in Dairy Flocks Reared in the Marche Region, Italy

Author

Attili Anna Rita, Ngu Ngwa Victor, Preziuso Silvia, Pacifici Luciana, Domesi Anastasia, and Cuteri Vincenzo

Subjects
Veterinary medicine, SF600-1100
Abstract

In order to fulfil the seroprevalence gap on Mycobacterium avium subsp. paratuberculosis infection in ovine dairy farms of Marche region (central Italy), a stratified study was carried out on 2086 adult female sheep randomly chosen from 38 herds selected in Ancona and Macerata provinces. 73.7% flocks resulted infected by a commercial ELISA test (Pourquier, France), with a mean seroprevalence of 6.29% of sampled sheep in both provinces. A higher number of MAP seropositive ewes was recorded in the large herds' consistence than in the small and medium herds' consistence (𝑃=0.0269), and a greater percentage of infected sheep was obtained among female at early/late than in peak lactation stage (𝑃=0.0237). MAP infection was confirmed in 12.6% of infected farms by faecal culture. The true sheep-level seroprevalence was 15.1% ± 7.3%.

Published
2011
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22. Denaturing Gradient Gel Electrophoresis Analysis of Bacteria in Italian Ticks and First Detection ofStreptococcus equiinRhipicephalus bursafrom the Lazio Region

Author

Cappelli, Alessia, primary, Capone, Aida, additional, Valzano, Matteo, additional, Bozic, Jovana, additional, Preziuso, Silvia, additional, Mensah, Priscilla, additional, Varotto Boccazzi, Ilaria, additional, Rinaldi, Laura, additional, Favia, Guido, additional, and Ricci, Irene, additional

Published
2019
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23. Estudio de la patogenia el Maedi-Visna ovino y su aplicación en el diagnóstico y control de la enfermedad = Study of ovine Maedi-Visna pathogenia and its application in diagnostic and illness control

Author

Gayo Roces, Elena, García Marín, Juan Francisco, Preziuso, Silvia, Sanidad Animal, Gayo Roces, Elena, Gayo Roces, Elena, García Marín, Juan Francisco, Preziuso, Silvia, Sanidad Animal, and Gayo Roces, Elena

Abstract

El Maedi-Visna (MV) es una enfermedad muy difundida a nivel mundial y en nuestro país, que tiene especial importancia en Castilla y León, donde causa importantes pérdidas económicas en ovino intensivo de leche de raza Assaf Española. La enfermedad, de curso crónico, pasa a menudo desapercibida hasta estados avanzados de la misma, especialmente la forma mamaria, dando lugar a una pérdida progresiva de la producción lechera. La falta de vacunas o tratamientos efectivos hacen que sea fundamental el contar con técnicas de diagnóstico sensibles que permitan conocer la situación epidemiológica de los rebaños y la detección precoz de los animales infectados para su eliminación en las sucesivas reposiciones. El objetivo general de esta tesis doctoral es contribuir al conocimiento de la patogenia del MV, describiendo la respuesta inmune del hospedador frente a la infección, así como la valoración de diferentes métodos de diagnóstico para la detección precoz y el estudio de las cepas circulantes. Se describen tres patrones de lesión en pulmón, glándula mamaria y SNC en función de la población celular predominante en el infiltrado inflamatorio: histiocítico, linfocítico y mixto. El patrón histiocítico se caracterizó por un predominio de macrófagos formando el infiltrado inflamatorio junto con algunos linfocitos B, por una respuesta serológica más intensa, caracterizada por altos títulos de anticuerpos, y por una mayor cantidad de virus en tejido mediante valoración subjetiva en IHQ. Al contrario, los animales con patrón linfocítico presentaron lesiones con un predominio de linfocitos T, títulos bajos de anticuerpos en test ELISA y una menor cantidad de virus en tejido. Por lo tanto, los animales con patrón histiocítico serían fácilmente detectables mediante test serológicos, mientras que aquellos con patrón linfocítico, incluso con valores negativos, pueden pasar desapercibidos en el rebaño. El patrón mixto, descrito por primera vez en esta tesis doctoral, se caracterizó por u

Published
2018

24. Estudio de la patogenia el Maedi-Visna ovino y su aplicación en el diagnóstico y control de la enfermedad = Study of ovine Maedi-Visna pathogenia and its application in diagnostic and illness control

Author

García Marín, Juan Francisco, Preziuso, Silvia, Sanidad Animal, Gayo Roces, Elena, García Marín, Juan Francisco, Preziuso, Silvia, Sanidad Animal, and Gayo Roces, Elena

Abstract

El Maedi-Visna (MV) es una enfermedad muy difundida a nivel mundial y en nuestro país, que tiene especial importancia en Castilla y León, donde causa importantes pérdidas económicas en ovino intensivo de leche de raza Assaf Española. La enfermedad, de curso crónico, pasa a menudo desapercibida hasta estados avanzados de la misma, especialmente la forma mamaria, dando lugar a una pérdida progresiva de la producción lechera. La falta de vacunas o tratamientos efectivos hacen que sea fundamental el contar con técnicas de diagnóstico sensibles que permitan conocer la situación epidemiológica de los rebaños y la detección precoz de los animales infectados para su eliminación en las sucesivas reposiciones. El objetivo general de esta tesis doctoral es contribuir al conocimiento de la patogenia del MV, describiendo la respuesta inmune del hospedador frente a la infección, así como la valoración de diferentes métodos de diagnóstico para la detección precoz y el estudio de las cepas circulantes. Se describen tres patrones de lesión en pulmón, glándula mamaria y SNC en función de la población celular predominante en el infiltrado inflamatorio: histiocítico, linfocítico y mixto. El patrón histiocítico se caracterizó por un predominio de macrófagos formando el infiltrado inflamatorio junto con algunos linfocitos B, por una respuesta serológica más intensa, caracterizada por altos títulos de anticuerpos, y por una mayor cantidad de virus en tejido mediante valoración subjetiva en IHQ. Al contrario, los animales con patrón linfocítico presentaron lesiones con un predominio de linfocitos T, títulos bajos de anticuerpos en test ELISA y una menor cantidad de virus en tejido. Por lo tanto, los animales con patrón histiocítico serían fácilmente detectables mediante test serológicos, mientras que aquellos con patrón linfocítico, incluso con valores negativos, pueden pasar desapercibidos en el rebaño. El patrón mixto, descrito por primera vez en esta tesis doctoral, se caracterizó por u

Published
2018

37. Denaturing Gradient Gel Electrophoresis Analysis of Bacteria in Italian Ticks and First Detection of Streptococcus equi in Rhipicephalus bursa from the Lazio Region.

Author

Cappelli, Alessia, Capone, Aida, Valzano, Matteo, Bozic, Jovana, Preziuso, Silvia, Mensah, Priscilla, Varotto Boccazzi, Ilaria, Rinaldi, Laura, Favia, Guido, and Ricci, Irene

Subjects
DENATURING gradient gel electrophoresis, CASTOR bean tick, STREPTOCOCCUS equi, RHIPICEPHALUS, TICKS, BROWN dog tick, TICK-borne diseases
Abstract

Tick-borne diseases are an increasing problem for the community. Ticks harbor a complex microbial population acquired while feeding on a variety of animals. Profiling the bacterial population by 16S rDNA amplification and denaturing gradient gel electrophoresis enables detection of the broad spectrum of bacteria that settles in the ticks. This study identified known and unknown tick-infecting bacteria in samples from Italy. Seven adult ticks from different hosts and origins were analyzed: two Rhipicephalus sanguineus ticks from dogs (Lombardia), two Rhipicephalus bursa ticks from bovines (Lazio), and three Ixodes ricinus ticks from humans (Marche). The major result was the first report of the zoonotic agent Streptococcus equi in ticks. S. equi is a species complex of highly contagious pathogens. Subsequent to S. equi detection in a R. bursa tick removed from a bovine of Lazio in 2012, we studied 95 R. bursa samples collected from 3 bovines, 3 ponies, and 1 sheep grazing in the same area in 2012 and from 6 ponies grazing there in 2017. The results of a specific PCR assay indicated a not sporadic occurrence of S. equi in ticks. This finding provides a basis for assessing the potential of ticks to harbor and disperse S. equi. [ABSTRACT FROM AUTHOR]

Published
2019
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38. Maedi-visna virus in Turkish sheep: a preliminary serological survey using ELISA tests

Author

PREZIUSO, Silvia, OR, Mehmet Erman, GIAMMARIOLI, Monica, and KAYAR, Abdullah

Subjects
Key words: ELISA,maedi-visna virus,sheep,Turkey,p25,gp44
Abstract

The maedi-visna virus (MVV) is distributed worldwide and, to date, no epidemiological surveys have been carried out using ELISA testing in Turkey. The aims of this study were as follows: i) to verify the diagnostic performance of a home-made ELISA test (HM-ELISA) using Turkish ovine sera in comparison with other commercially available ELISA tests, and ii) to perform a preliminary epidemiological survey in İstanbul province. The MVV seroprevalence in İstanbul province was 15.3%. Older sheep were 5 times more likely to be positive than younger animals, ewes were 3 times more likely to be positive than rams, and all the Red Karaman and White Karaman sheep tested were seronegative. The performance of the HM-ELISA and that of the other commercial tests using Turkish ovine serum samples was similar; therefore, the HM-ELISA test is suitable for use in extensive epidemiological surveys and eradication programs in Turkey.

Published
2014

40. Beta-hemolytic Streptococcus dysgalactiae strains isolated from horses are a genetically distinct population within the Streptococcus dysgalactiae taxon

Author

Pinho, Marcos D., primary, Erol, Erdal, additional, Ribeiro-Gonçalves, Bruno, additional, Mendes, Catarina I., additional, Carriço, João A., additional, Matos, Sandra C., additional, Preziuso, Silvia, additional, Luebke-Becker, Antina, additional, Wieler, Lothar H., additional, Melo-Cristino, Jose, additional, and Ramirez, Mario, additional

Published
2016
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