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Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous Brillouin scattering results in weak signals typically requiring prolonged exposure times or illumination dosages potentially harmful for biological samples. Here, we present a new approach for highly-multiplexed, and therefore rapid, spectral acquisition of the Brillouin scattered light. Specifically, by exploiting a custom-built Fourier-transform imaging spectrometer and the symmetric properties of the Brillouin spectrum, we experimentally demonstrate full-field 2D spectral Brillouin imaging of phantoms as well as biological samples, at a throughput of up to 40,000 spectra per second over a ~300um field-of-view. This represents an approximately three orders of magnitude improvement in speed and throughput compared to standard confocal methods while retaining high spatial resolution and the capability to acquire three-dimensional images of photosensitive samples in biology and medicine., Comment: 20 pages, 4 main figures, 3 supplementary figures

Over the past decade, optical wavefront shaping has been developed to focus light through highly opaque scattering layers, opening new possibilities for biomedical applications. To probe light intensity deep inside soft scattering media such as biological tissues, internal guide-stars are required. Here, we give an overview of the main principles and describe in depth the use of a photoacoustic feedback signal for this purpose. We further present first principles calculations and simulations to estimate important experimental parameters, and detailed instructions on designing and conducting these experiments. Finally, we provide guidance towards selecting suitable equipment for building a typical experimental setup, paving the way for further innovative biomedical imaging and therapy applications., Comment: 25 pages, 9 figures, 3 tables

Two-photon microscopy (2PM) has become an important tool in biology to study the structure and function of intact tissues in-vivo. However, adult mammalian tissues such as the mouse brain are highly scattering, thereby putting fundamental limits on the achievable imaging depth, which typically resides around 600-800um. In principle, shifting both the excitation as well as (fluorescence) emission light to the shortwave near-infrared (SWIR, 1000-1700 nm) region promises substantially deeper imaging in 2PM, yet has proven challenging in the past due to the limited availability of detectors and probes in this wavelength region. To overcome these limitations and fully capitalize on the SWIR region, in this work we introduce a novel array of superconducting nanowire single-photon detectors (SNSPDs) and associated custom detection electronics for the use in near-infrared 2PM. The SNSPD array exhibits high efficiency and dynamic range, as well as low dark-count rates over a wide wavelength range. Additionally, the electronics and software permit seamless integration into typical 2PM systems. Together with a fluorescent dye emitting at 1105 nm, we report imaging depth of > 1.1mm in the in-vivo mouse brain, limited only by available labeling density and laser power. Our work further establishes SWIR 2PM approaches and SNSPDs as promising technologies for deep tissue biological imaging., Comment: 17 pages, 5+1 figures

All-optical ultrasound detection bears unique advantages for photoacoustics, including wider detection bandwidth, higher signal-to-noise per unit area and lower susceptibility to electromagnetic noise. These benefits have established optical ultrasound detection as a key method for photoacoustic applications in biology and medicine. However, the use of free-space detectors renders this approach sensitive to optical aberrations, which can degrade the pressure sensitivity and result in deteriorated image quality. While spatial mode-filtering through fibers has been proposed to alleviate these problems in Fabry-P\'erot based pressure sensors, their real functional advantage has never been properly investigated. In this paper we rigorously and quantitatively compare the performance of free-space and fibre-coupled detectors in a custom correlative setup. We demonstrate the superiority of the latter in terms of both signal level and image quality in realistic all-optical photoacoustic tomography settings., Comment: 6 pages, 2 figures

Zernike polynomials are widely used mathematical models of experimentally observed optical aberrations. Their useful mathematical properties, in particular their orthogonality, make them a ubiquitous basis set for solving various problems in beam optics. Thus they have found widespread use in adaptive optics realizations that are used to correct wavefront aberrations. However, Zernike aberrations lose their orthogonality when used in combination with Gaussian beams, which are omnipresent in real-world optical applications. As a consequence, Zernike aberrations in Gaussian beams start to cross-couple between each other, a phenomenon that does not occur for Zernike aberrations in plane waves. Here, we describe how the aberration radius influences this cross-coupling of Zernike aberrations. Furthermore, we propose that this effect can actually be harnessed to allow efficient compensation of higher-order aberrations using only low-order Zernike modes. This finding has important practical implications, as it suggests the possibility of using adaptive optics devices with low element numbers to compensate aberrations which would normally require more complex and expensive devices., Comment: 12 pages, 3 figures, including Supplementary Information

Optical resonators are one of the most promising optical devices for manufacturing high-performance pressure sensors for photoacoustic imaging. Among these, Fabry-P\'erot (FP) based pressure sensors have been successfully used for a multitude of applications. However, critical performance aspects of FP based pressure sensors have not been extensively studied, including the effects system parameters such as beam diameter and cavity misalignment have on transfer function shape. Here, we discuss the possible origins of the transfer function asymmetry, the impact it has on measurement sensitivity as well as ways to correctly estimate the FP pressure sensitivity under practical experimental conditions., Comment: 6 pages, 2 figures

Zernike polynomials are one of the most widely used mathematical descriptors of optical aberrations in the fields of imaging and adaptive optics. Their mathematical orthogonality as well as isomorphisms with experimentally observable aberrations make them a very powerful tool in solving numerous problems in beam optics. However, Zernike aberrations show cross-coupling between individual modes when used in combination with Gaussian beams, an effect that has not been extensively studied. Here we propose a novel framework that is capable of explaining the fundamental cross-compensation of Zernike type aberrations, both in low-aberration and high-aberration regimes. Our approach is based on analysing the coupling between Zernike modes and different classes of Laguerre-Gauss modes which allows investigating aberrated beams not only on a single plane but also during their 3D propagation.

Fabry-P\'erot (FP) cavities are fundamental and ubiquitous optical elements frequently used in various sensing applications. Here, we introduce a general theoretical framework to study arbitrary light-cavity mode interactions for planar FPs and show how optical aberrations, intrinsic to the interrogating beam or due to imperfect cavities, reduce optical sensitivity by exciting higher-order spatial modes in the cavity. We find that particular Zernike aberrations play a dominant role in sensitivity degradation, and that the general loss of sensitivity can be significantly recovered by appropriate wavefront correction or mode filtering. We then demonstrate our theoretical findings also experimentally and show that in practice the sensitivity of realistic planar FP sensors can be improved up to three-fold by a synergistic combination of adaptive optics and passive mode filtering., Comment: includes Supplementary Information

The role and importance of mechanical properties of cells and tissues in cellular function, development as well as disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations. Recently, a new type of optical elastography, namely Brillouin microscopy, has emerged as a non-destructive, label- and contact-free method which can probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. This has led to increased attention amongst the biological and medical research communities, but also to debates about the interpretation and relevance of the measured physical quantities. Here, we review this emerging technology by describing the underlying biophysical principles and discussing the interpretation of Brillouin spectra arising from heterogeneous biological matter. We further elaborate on the technique's limitations as well as its potential for new insights in biology in order to guide interested researchers from various fields., Comment: Review article, 19 pages, 4 figures

A number of questions in systems biology such as understanding how dynamics of neuronal networks are related to brain function require the ability to capture the functional dynamics of large cellular populations at high speed. Recently, this has driven the development of a number of parallel and high speed imaging techniques such as light-sculpting microscopy, which has been used to capture neuronal dynamics at the whole brain and single cell level in small model organism. However, the broader applicability of light-sculpting microscopy is limited by the size of volumes for which high speed imaging can be obtained and scattering in brain tissue. Here, we present strategies for optimizing the present tradeoffs in light-sculpting microscopy. Various scanning modalities in light-sculpting microscopy are theoretically and experimentally evaluated, and strategies to maximize the obtainable volume speeds, and depth penetration in brain tissue using different laser systems are provided. Design-choices, important parameters and their trade-offs are experimentally demonstrated by performing calcium-imaging in acute mouse-brain slices. We further show that synchronization of line-scanning techniques with rolling-shutter read-out of the camera can reduce scattering effects and enhance image contrast at depth., Comment: 16 pages, 5 figures

Recent efforts in neuroscience research seek to obtain detailed anatomical neuronal wiring maps as well as information on how neurons in these networks engage in dynamic activities. Although the entire connectivity map of the nervous system of C. elegans has been known for more than 25 years, this knowledge has not been sufficient to predict all functional connections underlying behavior. To approach this goal, we developed a two-photon technique for brain-wide calcium imaging in C. elegans using wide-field temporal focusing (WF-TEFO). Pivotal to our results was the use of a nuclear-localized, genetically encoded calcium indicator (NLS-GCaMP5K) that permits unambiguous discrimination of individual neurons within the densely-packed head ganglia of C. elegans. We demonstrate near-simultaneous recording of activity of up to 70% of all head neurons. In combination with a lab-on-a-chip device for stimulus delivery, this method provides an enabling platform for establishing functional maps of neuronal networks., Comment: 28 pages, 5 figures, plus Supplementary Information (24 pages, 10 figures)

We demonstrate a novel scheme for femto-second pulsed spontaneous parametric down-conversion in periodically poled KTP crystals. Our scheme is based on a crossed crystal configuration with collinear quasi-phase-matching. The non-degenerate photon pairs are split in a fiber-based wavelength division multiplexer. The source is easier to align than common pulsed sources based on bulk BBO crystals and exhibits high-quality polarization entanglement as well as non-classical interference capabilities. Hence, we expect this source to be a well-suited candidate for multi-photon state generation e.g. for linear optical quantum computation and quantum communication networks., Comment: 4 pages, 4 figures

We experimentally demonstrate a single-qubit decohering quantum channel using linear optics. We implement the channel, whose special cases include both the amplitude-damping channel and the bit-flip channel, using a single, static optical setup. Following a recent theoretical result [M. Piani et al., Phys. Rev. A, 84, 032304 (2011)], we realize the channel in an optimal way, maximizing the probability of success, i.e., the probability for the photonic qubit to remain in its encoding. Using a two-photon entangled resource, we characterize the channel using ancilla-assisted process tomography and find average process fidelities of 0.9808 \pm 0.0002 and 0.9762 \pm 0.0002 for amplitude-damping and the bit-flip case, respectively., Comment: 4 pages, 3 figures

Heisenberg's uncertainty principle provides a fundamental limitation on an observer's ability to simultaneously predict the outcome when one of two measurements is performed on a quantum system. However, if the observer has access to a particle (stored in a quantum memory) which is entangled with the system, his uncertainty is generally reduced. This effect has recently been quantified by Berta et al. [Nature Physics 6, 659 (2010)] in a new, more general uncertainty relation, formulated in terms of entropies. Using entangled photon pairs, an optical delay line serving as a quantum memory and fast, active feed-forward we experimentally probe the validity of this new relation. The behaviour we find agrees with the predictions of quantum theory and satisfies the new uncertainty relation. In particular, we find lower uncertainties about the measurement outcomes than would be possible without the entangled particle. This shows not only that the reduction in uncertainty enabled by entanglement can be significant in practice, but also demonstrates the use of the inequality to witness entanglement., Comment: 8 pages, 4 figures, comments welcome

A significant limitation of practical quantum key distribution (QKD) setups is currently their limited operational range. It has recently been emphasized (X. Ma, C.-H. F. Fung, and H.-K. Lo., Phys. Rev. A, 76:012307, 2007) that entanglement-based QKD systems can tolerate higher channel losses than systems based on weak coherent laser pulses (WCP), in particular when the source is located symmetrically between the two communicating parties, Alice and Bob. In the work presented here, we experimentally study this important advantage by implementing different entanglement-based QKD setups on a 144~km free-space link between the two Canary Islands of La Palma and Tenerife. We established three different configurations where the entangled photon source was placed at Alice's location, asymmetrically between Alice and Bob and symmetrically in the middle between Alice and Bob, respectively. The resulting quantum channel attenuations of 35~dB, 58~dB and 71~dB, respectively, significantly exceed the limit for WCP systems. This confirms that QKD over distances of 300~km and even more is feasible with entangled state sources placed in the middle between Alice and Bob., Comment: 14 pages, 5 figures, 1 table

Quantum entanglement enables tasks not possible in classical physics. Many quantum communication protocols require the distribution of entangled states between distant parties. Here we experimentally demonstrate the successful transmission of an entangled photon pair over a 144 km free-space link. The received entangled states have excellent, noise-limited fidelity, even though they are exposed to extreme attenuation dominated by turbulent atmospheric effects. The total channel loss of 64 dB corresponds to the estimated attenuation regime for a two-photon satellite quantum communication scenario. We confirm that the received two-photon states are still highly entangled by violating the CHSH inequality by more than 5 standard deviations. From a fundamental point of view, our results show that the photons are virtually not subject to decoherence during their 0.5 ms long flight through air, which is encouraging for future world-wide quantum communication scenarios., Comment: 5 pages, 3 figures, replaced paper with published version, added journal reference

We propose a link between logical independence and quantum physics. We demonstrate that quantum systems in the eigenstates of Pauli group operators are capable of encoding mathematical axioms and show that Pauli group quantum measurements are capable of revealing whether or not a given proposition is logically dependent on the axiomatic system. Whenever a mathematical proposition is logically independent of the axioms encoded in the measured state, the measurement associated with the proposition gives random outcomes. This allows for an experimental test of logical independence. Conversely, it also allows for an explanation of the probabilities of random outcomes observed in Pauli group measurements from logical independence without invoking quantum theory. The axiomatic systems we study can be completed and are therefore not subject to Goedel's incompleteness theorem., Comment: 9 pages, 4 figures, published version plus additional experimental appendix

Entanglement swapping allows to establish entanglement between independent particles that never interacted nor share any common past. This feature makes it an integral constituent of quantum repeaters. Here, we demonstrate entanglement swapping with time-synchronized independent sources with a fidelity high enough to violate a Clauser-Horne-Shimony-Holt inequality by more than four standard deviations. The fact that both entangled pairs are created by fully independent, only electronically connected sources ensures that this technique is suitable for future long-distance quantum communication experiments as well as for novel tests on the foundations of quantum physics., Comment: added technical details and extended introduction and conclusion, slightly modified the abstract, corrected a mistake in the affiliations

Entanglement is an essential resource in current experimental implementations for quantum information processing. We review a class of experiments exploiting photonic entanglement, ranging from one-way quantum computing over quantum communication complexity to long-distance quantum communication. We then propose a set of feasible experiments that will underline the advantages of photonic entanglement for quantum information processing., Comment: 33 pages, 4 figures, OSA style

We report the first demonstration of a quantum game on an all-optical one-way quantum computer. Following a recent theoretical proposal we implement a quantum version of Prisoner's Dilemma, where the quantum circuit is realized by a 4-qubit box-cluster configuration and the player's local strategies by measurements performed on the physical qubits of the cluster. This demonstration underlines the strength and versatility of the one-way model and we expect that this will trigger further interest in designing quantum protocols and algorithms to be tested in state-of-the-art cluster resources., Comment: 13 pages, 4 figures

As information carriers in quantum computing, photonic qubits have the advantage of undergoing negligible decoherence. However, the absence of any significant photon-photon interaction is problematic for the realization of non-trivial two-qubit gates. One solution is to introduce an effective nonlinearity by measurements resulting in probabilistic gate operations. In one-way quantum computation, the random quantum measurement error can be overcome by applying a feed-forward technique, such that the future measurement basis depends on earlier measurement results. This technique is crucial for achieving deterministic quantum computation once a cluster state (the highly entangled multiparticle state on which one-way quantum computation is based) is prepared. Here we realize a concatenated scheme of measurement and active feed-forward in a one-way quantum computing experiment. We demonstrate that, for a perfect cluster state and no photon loss, our quantum computation scheme would operate with good fidelity and that our feed-forward components function with very high speed and low error for detected photons. With present technology, the individual computational step (in our case the individual feed-forward cycle) can be operated in less than 150 ns using electro-optical modulators. This is an important result for the future development of one-way quantum computers, whose large-scale implementation will depend on advances in the production and detection of the required highly entangled cluster states., Comment: 19 pages, 4 figures