1. In situ hybridization (ISH) in preparasitic and parasitic stages of the plant-parasitic nematode Meloidogyne spp
- Author
-
Marie-Noëlle Rosso, Maëlle Jaouannet, Stéphanie Jaubert-Possamai, C. Nguyen, Michaël Quentin, Bruno Favery, Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), UMR 1355 ISA, Institut National de la Recherche Agronomique (INRA), Université de Nice Sophia-Antipolis (UNSA), Biodiversité et Biotechnologie Fongiques (BBF), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), INRA, CNRS, University of Nice Sophia Antipolis, the Region Provence-Alpes-Côte d’Azur, the French Government, the European Union and NATO Collaborative Research Grant. C-N.N. was supported by USTH fellowships, 911-USTH program, from the Ministry of Education and Training of The Socialist Republic of Vietnam., Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM), École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Favery, Bruno, Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,0301 basic medicine ,Strategy and Management ,[SDV]Life Sciences [q-bio] ,Gene expression pattern ,Plant pathogen ,Preparasitic and parasitic stages ,mRNAs ,In situ hybridization ,Biology ,01 natural sciences ,Industrial and Manufacturing Engineering ,03 medical and health sciences ,chemistry.chemical_compound ,Dig ,Methods Article ,Parasite hosting ,Digoxigenin ,Gene ,Mechanical Engineering ,Hybridization probe ,Metals and Alloys ,RNA ,virus diseases ,biology.organism_classification ,Molecular biology ,030104 developmental biology ,Nematode ,chemistry ,010606 plant biology & botany - Abstract
The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the plant parasite Meloidogyne spp. (root-knot nematode) to visualize RNAs both in free-living preparasitic juveniles and in parasitic stages settled in the plant tissues. We describe each step of the probe synthesis, digoxigenin (DIG) labeling, nematode extraction from plant tissue, and ISH procedure.
- Published
- 2018
- Full Text
- View/download PDF