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2 results on '"Preimplantation failure"'

1. The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility

Author

Obah A. Ojarikre, Shantha K. Mahadevaiah, Nadège Vernet, Maria Szot, Fanny Decarpentrie, Áine Rattigan, Paul S. Burgoyne, Peter J.I. Ellis, and Teruko Taketo

Subjects
Infertility, Preimplantation failure, Genotype, Mouse, media_common.quotation_subject, Cleavage Stage, Ovum, Blotting, Western, Sex Chromosome Disorders of Sex Development, Fertility, Mice, Transgenic, XY female infertility, Biology, Breeding, Y chromosome, Andrology, Mice, Meiosis 2 defects, Zfy2, Meiosis, Y Chromosome, medicine, Animals, First cleavage, Molecular Biology, Crosses, Genetic, Research Articles, media_common, Gene Expression Profiling, Embryo, medicine.disease, Oocyte, Microarray Analysis, Molecular biology, Sex-Determining Region Y Protein, DNA-Binding Proteins, Testis determining factor, medicine.anatomical_structure, Gene Expression Regulation, Y gene expression in oocytes, Y linkage, Linear Models, Oocytes, Female, Infertility, Female, Developmental Biology, Transcription Factors
Abstract

Outbred XY(Sry-) female mice that lack Sry due to the 11 kb deletion Sry(dl1Rlb) have very limited fertility. However, five lines of outbred XY(d) females with Y chromosome deletions Y(Del(Y)1Ct)-Y(Del(Y)5Ct) that deplete the Rbmy gene cluster and repress Sry transcription were found to be of good fertility. Here we tested our expectation that the difference in fertility between XO, XY(d-1) and XY(Sry-) females would be reflected in different degrees of oocyte depletion, but this was not the case. Transgenic addition of Yp genes to XO females implicated Zfy2 as being responsible for the deleterious Y chromosomal effect on fertility. Zfy2 transcript levels were reduced in ovaries of XY(d-1) compared with XY(Sry-) females in keeping with their differing fertility. In seeking the biological basis of the impaired fertility we found that XY(Sry-), XY(d-1) and XO,Zfy2 females produce equivalent numbers of 2-cell embryos. However, in XY(Sry-) and XO,Zfy2 females the majority of embryos arrested with 2-4 cells and almost no blastocysts were produced; by contrast, XY(d-1) females produced substantially more blastocysts but fewer than XO controls. As previously documented for C57BL/6 inbred XY females, outbred XY(Sry-) and XO,Zfy2 females showed frequent failure of the second meiotic division, although this did not prevent the first cleavage. Oocyte transcriptome analysis revealed major transcriptional changes resulting from the Zfy2 transgene addition. We conclude that Zfy2-induced transcriptional changes in oocytes are sufficient to explain the more severe fertility impairment of XY as compared with XO females.

Published
2014

2. The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects, a high incidence of subsequent early cleavage stage arrest and infertility.

Author

Vernet N, Szot M, Mahadevaiah SK, Ellis PJ, Decarpentrie F, Ojarikre OA, Rattigan Á, Taketo T, and Burgoyne PS

Subjects
Animals, Blotting, Western, Breeding, Cleavage Stage, Ovum pathology, Cleavage Stage, Ovum physiology, Crosses, Genetic, DNA-Binding Proteins genetics, Female, Gene Expression Profiling, Gene Expression Regulation genetics, Genotype, Linear Models, Mice, Mice, Transgenic, Microarray Analysis, Transcription Factors genetics, DNA-Binding Proteins metabolism, Infertility, Female genetics, Meiosis genetics, Oocytes metabolism, Sex Chromosome Disorders of Sex Development genetics, Sex-Determining Region Y Protein deficiency, Transcription Factors metabolism, Y Chromosome genetics
Abstract

Outbred XY(Sry-) female mice that lack Sry due to the 11 kb deletion Sry(dl1Rlb) have very limited fertility. However, five lines of outbred XY(d) females with Y chromosome deletions Y(Del(Y)1Ct)-Y(Del(Y)5Ct) that deplete the Rbmy gene cluster and repress Sry transcription were found to be of good fertility. Here we tested our expectation that the difference in fertility between XO, XY(d-1) and XY(Sry-) females would be reflected in different degrees of oocyte depletion, but this was not the case. Transgenic addition of Yp genes to XO females implicated Zfy2 as being responsible for the deleterious Y chromosomal effect on fertility. Zfy2 transcript levels were reduced in ovaries of XY(d-1) compared with XY(Sry-) females in keeping with their differing fertility. In seeking the biological basis of the impaired fertility we found that XY(Sry-), XY(d-1) and XO,Zfy2 females produce equivalent numbers of 2-cell embryos. However, in XY(Sry-) and XO,Zfy2 females the majority of embryos arrested with 2-4 cells and almost no blastocysts were produced; by contrast, XY(d-1) females produced substantially more blastocysts but fewer than XO controls. As previously documented for C57BL/6 inbred XY females, outbred XY(Sry-) and XO,Zfy2 females showed frequent failure of the second meiotic division, although this did not prevent the first cleavage. Oocyte transcriptome analysis revealed major transcriptional changes resulting from the Zfy2 transgene addition. We conclude that Zfy2-induced transcriptional changes in oocytes are sufficient to explain the more severe fertility impairment of XY as compared with XO females.

Published
2014
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