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Kaustav Banerjee,1 Subhasish Pramanik,2 Lakshmi Kanta Mondal3 1Decision Sciences Area, Indian Institute of Management Lucknow, Uttar Pradesh, 226013, India; 2Department of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM Hospital, Kolkata, West Bengal, 700020, India; 3Department of Ophthalmology, Regional Institute of Ophthalmology, Medical College Campus, Kolkata, West Bengal, 700073, IndiaCorrespondence: Lakshmi Kanta Mondal, Department of Ophthalmology, Regional Institute of Ophthalmology, Medical College Campus, Kolkata, West Bengal, 700073, India, Tel +9830830216, Email Lakshmi.mondal62@gmail.comIntroduction: The focus is often on the best and worst eyes to detect early predictive and non-invasive biomarkers of diabetic retinopathy. Typically, such data have been dealt with in a case-control setting, which applies two-sample tests and ignores the correlation between the fellow eyes. Practitioners are mostly unaware that such measurements hide the labels of the fellow eyes, which rules out standard tools, such as paired t or signed-rank tests.Methods: This report discusses the problems with such data on best and worst eye measurements, and illustrates alternative paired tests for equality of means or locations using a case-control dataset.Results: This report illustrates that methods which ignore the correlation between fellow eyes result in grossly conservative tests. A battery of Z-tests which consider this correlation can resolve this issue.Discussion: This finding emphasizes the importance of selecting an appropriate control group for the detection of possible markers. Further, it cites an example to show that using data from fellow eyes and adjusting for their correlation may not always be the best option, contrary to common perception.Keywords: best eye, worst eye, unordered pairs

Subhasish Pramanik,1,* Chinmay Saha,1,2,* Subhankar Chowdhury,1 Chiranjit Bose,1 Nitai P Bhattacharyya,1 Lakshmi Kanta Mondal3 1Department of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM Hospital, Kolkata, 700020, West Bengal, India; 2Genome Science, School of Interdisciplinary Studies, University of Kalyani, Nadia, 741235, West Bengal, India; 3Department of Ophthalmology, Regional Institute of Ophthalmology, Medical College Campus, Kolkata, 700 073, West Bengal, India*These authors contributed equally to this workCorrespondence: Subhankar ChowdhuryDepartment of Endocrinology & Metabolism, Institute of Post Graduate Medical Education & Research and SSKM Hospital, Kolkata, 700020, West Bengal, India, Email subhankar.chowdhury@gmail.comLakshmi Kanta MondalDepartment of Ophthalmology, Regional Institute of Ophthalmology, Medical College Campus, 88, College Street, Kolkata, 700 073, West Bengal, India, Email lakshmi.mondal62@gmail.comPurpose: Diabetic retinopathy (DR), the leading cause of blindness among working adults, is an urgent public health problem as diabetes mellitus (DM) is increasing at an alarming rate. Hyperglycemia-induced endothelial dysfunction is the principal contributing factor leading to the development of microangiopathy. Altered levels of microRNA (miR), the negative regulator of protein-coding genes, have been observed and considered to be markers for DR. Present study aimed to find out whether miR levels in plasma could be effective biomarkers to differentiate between type 2 diabetes mellitus (T2DM) with non-proliferative retinopathy (NPDR) from T2DM with no-DR (DNR).Methods: We recruited 50 T2DM subjects comprising 31 NPDR and 19 DNR individuals. Surrogate markers of systemic oxidative stress and vascular endothelial growth factor (VEGF) were measured in plasma. Levels of miR-126 and miR-132 were determined in plasma and vitreous fluid using real-time PCR.Results: We observed that levels of miR-126 and miR-132 were decreased in NPDR subjects in comparison to DNR. Plasma levels of miRs were inversely correlated with secreted levels of VEGF and oxidative stress marker. The levels of these miRs showed discriminating ability between NPDR and DNR.Conclusion: Circulating miRs 126 and 132 in plasma or vitreous may serve as biomarkers for early diabetic retinopathy risk prediction, provided validated in a larger cohort and other forms of retinal vasculopathy or retinopathy in the future.Keywords: diabetic retinopathy, microRNA-126, microRNA-132, oxidative stress, malondialdehyde, vascular endothelial growth factor

Subhasish Pramanik,1 Lakshmi Kanta Mondal,2 Suman Kalyan Paine,3 Sneha Jain,2 Subhankar Chowdhury,1 Upasana Ganguly,4 Sayantan Ghosh,2 Chiranjit Bose,1 Koena Bhattacharjee,2 Gautam Bhaduri5 1Institute of Post Graduate Medical Education and Research (IPGMER), Kolkata, 700020, India; 2Regional Institute of Ophthalmology (RIO), Medical College and Hospital, Kolkata, 700073, India; 3National Institute of Biomedical Genomics (NIBMG), Kalyani, West Bengal, India; 4Maharishi Markandeshwar Institute of Medical Sciences and Research, Mullana, Haryana, India; 5MGM Medical College, Kishanganj, Bihar, IndiaCorrespondence: Lakshmi Kanta MondalRegional Institute of Ophthalmology, Medical College, Kolkata, IndiaTel +91 9830830216Email Lakshmi.mondal62@gmail.comPurpose: In the present study, we aimed to evaluate the efficacy, safety, and cost-effectiveness of the anti-vascular endothelial growth factor (anti-VEGF), namely ranibizumab (RBZ) or bevacizumab (BVZ), after either focal or grid or scatter laser photocoagulation, for the treatment of diabetic macular edema (DME) in the Indian population.Methods: Retrospective data were collected in the Regional Institute of Ophthalmology, Kolkata, India between January 2018 and June 2019. Seventy-seven eyes received 3 consecutive monthly intravitreal injections of RBZ (0.5 mg) and were followed by prompt laser photocoagulation (within 7– 10 days after the third injection). Similarly, 51 eyes received 3 consecutive monthly intravitreal injections of BVZ (1.25 mg), an off-label drug, and were followed by prompt laser therapy. Safety assessments of the therapy, as well as surrogate markers of biochemical derangements related to diabetic retinopathy (DR), were also investigated at the end of 12 months.Results: Seventy-seven subjects who were given a treatment of RBZ+laser therapy showed average 6.87± 5.53 letters gain in their best-corrected visual acuity (BCVA) score, whereas the ones treated with off-label BVZ+ laser therapy demonstrated improvement in BCVA of an average 6.82± 5.76 letters in “Early Treatment Diabetic Retinopathy Study” (ETDRS) chart. The study also highlights the cost-effectiveness of both RBZ+laser and BVZ+laser therapies for the treatment of DME in DR. The results demonstrated that a subject has to pay 20.951 times more cost (in INR) for RBZ+laser therapy compared to BVZ+laser therapy, to get an almost similar outcome.Conclusion: BVZ is found to be the more attractive option for treating DME in DR for its cost-friendliness over RBZ in terms of BCVA outcome, as well as the safety perspectives, at least for the economically backward population in developing countries, like India.Keywords: diabetic retinopathy, diabetic macular edema, ranibizumab, bevacizumab, laser therapy, cost-effectiveness

Exploitation of heterosis is one of the potential means for improvement of sweet pepper (Capsicum annuum var. grossum L.) that can further be utilized for identification of desirable recombinants. Promising hybrids could be acceptable to growers of tropical and subtropical climates if it is a high yielder with attractive fruit colour and blocky shape. Seven diverse parents were selected through multivariate analysis and were crossed in half diallel mating design to determine the extent of heterosis, mode of gene action, combining ability effects, and dominance estimates for 18 quantitative characters. Expression of fruit colour and shape at physiological maturity stage in the F1 generation indicated the dominance of red coloured group over other fruit colour groups and dominance of blocky fruit shape over elongate and round fruit shape. Preponderance of non-additive gene action for most of the characters under study suggested the usefulness of exploitation of hybrid vigour. Three genotypes, 8/4, C/4, and Baby Bell were found most promising donors. The hybrids, Arya×Baby Bell, 8/4×Baby Bell and C/4×8/4 were identified as promising based on per se performance, heterosis manifested, and relevance of specific combining ability effects, for possible commercialization under low cost protected structure of tropical and subtropical climates after critical testing. Partial to over dominance response in inheritance of most traits contributed to the genetic basis of heterosis. Isolation of pure lines from the segregating generation of heterotic hybrids emerged as a promising approach to develop line-bred variety having improved fruit yield and quality. [ABSTRACT FROM AUTHOR]

Non-Newtonian fluid flow in pipe bends is inevitable in industrial applications. Previous researchers have extensively explored Newtonian flow through curved ducts. However, the non-Newtonian counterpart gets little attention. We study the turbulent flow of shear-dependent fluids obeying the Power-Law model in a pipe manifold containing an in-plane double bend. Ostwald-de Waele's power law is used to model the fluid's rheology. We utilize computational fluid dynamics (CFD) to solve Reynolds-averaged Navier-Stokes (RANS) equations with the k-ε turbulence model. We validate our numerical results with previous experimental results. The in-plane double bend perturbs the flow in the pipe manifold to develop a Prandtl's secondary flow of the first kind. A fully developed flow at the bend upstream is disturbed due to the bend's curvature and regains its fully developed characteristics upon a certain downstream length after the exit of the bend. We study the rheological characteristics of the secondary flow within the bend and the evolution of fluid flow at the bend downstream. We demonstrate that the centrifugal force-dominated secondary flow increases with a decrease of the non-Newtonian power-law index. We capture the camel'sback-shaped velocity profiles within the bend due to accelerating-decelerating flow. The study reveals that the average flow velocity increases along the bend with a corresponding pressure head loss. We quantify this velocity rise by a newly introduced non-dimensional number, viz. enhancement ratio. The double bend's enhancement ratio decreases with an increase in n. [ABSTRACT FROM AUTHOR]