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121 results on '"Pouli, Dimitra"'

1. A multimodal generative AI copilot for human pathology

Author

Lu, Ming Y., Chen, Bowen, Williamson, Drew F. K., Chen, Richard J., Zhao, Melissa, Chow, Aaron K., Ikemura, Kenji, Kim, Ahrong, Pouli, Dimitra, Patel, Ankush, Soliman, Amr, Chen, Chengkuan, Ding, Tong, Wang, Judy J., Gerber, Georg, Liang, Ivy, Le, Long Phi, Parwani, Anil V., Weishaupt, Luca L., and Mahmood, Faisal

Published
2024
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3. Imaging mitochondrial dynamics in human skin reveals depth-dependent hypoxia and malignant potential for diagnosis

Author

Pouli, Dimitra, Balu, Mihaela, Alonzo, Carlo A, Liu, Zhiyi, Quinn, Kyle P, Rius-Diaz, Francisca, Harris, Ronald M, Kelly, Kristen M, Tromberg, Bruce J, and Georgakoudi, Irene

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Bioengineering, Detection, screening and diagnosis, 4.1 Discovery and preclinical testing of markers and technologies, Biomarkers, Carcinoma, Basal Cell, Epidermis, Homeostasis, Humans, Hypoxia, Keratinocytes, Melanoma, Microscopy, Fluorescence, Multiphoton, Mitochondria, Mitochondrial Dynamics, NAD, Oxygen, Photons, Skin, Biological Sciences, Medical and Health Sciences, Medical biotechnology, Biomedical engineering
Abstract

Active changes in mitochondrial structure and organization facilitate cellular homeostasis. Because aberrant mitochondrial dynamics are implicated in a variety of human diseases, their assessment is potentially useful for diagnosis, therapy, and disease monitoring. Because current techniques for evaluating mitochondrial morphology are invasive or necessitate mitochondria-specific dyes, their clinical translation is limited. We report that mitochondrial dynamics can be monitored in vivo, within intact human skin by relying entirely on endogenous two-photon-excited fluorescence from the reduced metabolic coenzyme nicotinamide adenine dinucleotide (NADH). We established the sensitivity of this approach with in vivo, fast temporal studies of arterial occlusion-reperfusion, which revealed acute changes in the mitochondrial metabolism and dynamics of the lower human epidermal layers. In vitro hypoxic-reperfusion studies validated that the in vivo outcomes were a result of NADH fluorescence changes. To demonstrate the diagnostic potential of this approach, we evaluated healthy and cancerous human skin epithelia. Healthy tissues displayed consistent, depth-dependent morphological and mitochondrial organization patterns that varied with histological stratification and intraepithelial mitochondrial protein expression. In contrast, these consistent patterns were absent in cancerous skin lesions. We exploited these differences to successfully differentiate healthy from cancerous tissues using a predictive classification approach. Collectively, these results demonstrate that our label-free, automated, near real-time assessments of mitochondrial organization-relying solely on endogenous contrast-could be useful for accurate, noninvasive in vivo diagnosis.

Published
2016

4. An unusually encountered folliculosebaceous cystic hamartoma clinically resembling an epidermal inclusion cyst.

Author

Neman, Sophia, Said, Jordan T., Trinidad, John, and Pouli, Dimitra

Abstract

Folliculosebaceous cystic hamartoma (FSCH) is a rare cutaneous hamartoma comprising epithelial and mesenchymal components. It typically appears as slow-growing, skin-coloured nodules on the face and scalp. We report a case of a mid-forties male with a 4mm nodule on his left temple, initially thought to clinically represent an epidermal inclusion cyst (EIC). Excisional biopsy results identified the lesion as FSCH, characterised by its unique dilated infundibulocystic configuration with radiating sebaceous lobules and associated mesenchymal elements. FSCH can be misdiagnosed for EIC due to similar clinical presentations but has distinct histological features. Surgical excision is the standard treatment, and the patient has remained recurrence-free since the procedure. This case underscores the importance of considering FSCH in the differential diagnosis of particularly facial and scalp nodules. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Supplementary Video 9 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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9. Supplementary Video 5 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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10. Supplementary Video 3 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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11. Supplementary Video 1 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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12. Supplementary Methods, Figure Legends, Video Legends from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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13. Supplementary Video 8 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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14. Supplementary Video 2 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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15. Data from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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16. Supplementary Video 6 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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17. Supplementary Figure 2 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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18. Supplementary Figure 4 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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19. Supplementary Video 7 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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20. Supplementary Figure 3 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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21. Supplementary Figure 1 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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22. Supplementary Video 4 from Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, primary, Quinn, Kyle P., primary, Pouli, Dimitra, primary, Sridharan, Gautham, primary, McLaughlin-Drubin, Margaret E., primary, Alonzo, Carlo, primary, Lee, Kyongbum, primary, Münger, Karl, primary, and Georgakoudi, Irene, primary

Published
2023
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29. Human Corneal Tissue Model for Nociceptive Assessments

Author

Siran, Wang, primary, Ghezzi, Chiara E., additional, Cairns, Dana M., additional, Pollard, Rachel E., additional, Chen, Ying, additional, Gomes, Rachel, additional, McKay, Tina B., additional, Pouli, Dimitra, additional, Jamali, Arsia, additional, Georgakoudi, Irene, additional, Funderburgh, James L., additional, Kenyon, Kenneth, additional, Hamrah, Pedram, additional, and Kaplan, David L., additional

Published
2018
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38. From the Classroom to Facebook

Author

Kousoulis, Antonis A., primary, Kympouropoulos, Stylianos P., additional, Pouli, Dimitra K., additional, Economopoulos, Konstantinos P., additional, and Vardavas, Constantine I., additional

Published
2016
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44. Noninvasive assessment of mitochondrial organization in three-dimensional tissues reveals changes associated with cancer development

Author

Xylas, Joanna, primary, Varone, Antonio, additional, Quinn, Kyle P., additional, Pouli, Dimitra, additional, McLaughlin-Drubin, Margaret E., additional, Thieu, Hong-Thao, additional, Garcia-Moliner, Maria L., additional, House, Michael, additional, Hunter, Martin, additional, Munger, Karl, additional, and Georgakoudi, Irene, additional

Published
2014
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45. Endogenous Two-Photon Fluorescence Imaging Elucidates Metabolic Changes Related to Enhanced Glycolysis and Glutamine Consumption in Precancerous Epithelial Tissues

Author

Varone, Antonio, primary, Xylas, Joanna, additional, Quinn, Kyle P., additional, Pouli, Dimitra, additional, Sridharan, Gautham, additional, McLaughlin-Drubin, Margaret E., additional, Alonzo, Carlo, additional, Lee, Kyongbum, additional, Münger, Karl, additional, and Georgakoudi, Irene, additional

Published
2014
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48. Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles.

Author

Ming Wang, Zuris, John A., Fantao Meng, Rees, Holly, Shuo Sun, Pu Deng, Yong Han, Xue Gao, Pouli, Dimitra, Qi Wu, Georgakoudi, Irene, Liu, David R., and Qiaobing Xu

Subjects
PROTEIN analysis, LIPIDS, NANOPARTICLES analysis, ENDOSOMES, GENETIC recombination
Abstract

A central challenge to the development of protein-based therapeutics is the inefficiency of delivery of protein cargo across the mammalian cell membrane, including escape from endosomes. Here we report that combining bioreducible lipid nanoparticles with negatively supercharged Cre recombinase or anionic Cas9:single-guide (sg)RNA complexes drives the electrostatic assembly of nanoparticles that mediate potent protein delivery and genome editing. These bioreducible lipids efficiently deliver protein cargo into cells, facilitate the escape of protein from endosomes in response to the reductive intracellular environment, and direct protein to its intracellular target sites. The delivery of supercharged Cre protein and Cas9:sgRNA complexed with bioreducible lipids into cultured human cells enables gene recombination and genome editing with efficiencies greater than 70%. In addition, we demonstrate that these lipids are effective for functional protein delivery into mouse brain for gene recombination in vivo. Therefore, the integration of this bioreducible lipid platform with protein engineering has the potential to advance the therapeutic relevance of protein-based genome editing. [ABSTRACT FROM AUTHOR]

Published
2016
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49. Fetal Brain Extracellular Matrix Boosts Neuronal Network Formation in 3D Bioengineered Model of Cortical Brain Tissue

Author

Sood, Disha, Chwalek, Karolina, Stuntz, Emily, Pouli, Dimitra, Du, Chuang, Tang-Schomer, Min, Georgakoudi, Irene, Black, Lauren D., and Kaplan, David L.

Abstract

The extracellular matrix (ECM) constituting up to 20% of the organ volume is a significant component of the brain due to its instructive role in the compartmentalization of functional microdomains in every brain structure. The composition, quantity, and structure of ECM changes dramatically during the development of an organism greatly contributing to the remarkably sophisticated architecture and function of the brain. Because fetal brain is highly plastic, we hypothesize that the fetal brain ECM may contain cues promoting neural growth and differentiation, highly desired in regenerative medicine. Thus, we studied the effect of brain-derived fetal and adult ECM complemented with matricellular proteins on cortical neurons using in vitro 3D bioengineered model of cortical brain tissue. The tested parameters included neuronal network density, cell viability, calcium signaling, and electrophysiology. Both adult and fetal brain ECM as well as matricellular proteins significantly improved neural network formation as compared to single-component, collagen I matrix. Additionally, the brain ECM improved cell viability and lowered glutamate release. The fetal brain ECM induced superior neural network formation, calcium signaling, and spontaneous spiking activity over adult brain ECM. This study highlights the difference in the neuroinductive properties of fetal and adult brain ECM and suggests that delineating the basis for this divergence may have implications for regenerative medicine.

Published
2016
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