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Micro- and nanoplastic (MNP) pollution in aquatic ecosystems requires investigation on its source, transport, and extent to assess and mitigate its risks. Chitosan is a potential biomolecule for water treatment, but its interaction with MNP is undefined. In this work, chitosan-nanoplastic interaction was explored in the laboratory under environmentally relevant conditions using polystyrene (PS) nanoplastic (NP) as model particle to identify conditions at which PS-chitosan interaction resulted in aggregation. Aggregation limits NP transport and allows separation of NP for targeted remediation. The effect of environmental conditions (pH, salinity, dissolved organic matter (DOM) content), chitosan particle size and NP surface modification on chitosan-NP interaction was studied at various chitosan doses. PS aggregated at chitosan doses as low as 0.2 % w/w, while higher doses of chitosan resulted in re-stabilization of NP in solution, restoring the particle size to its initial value. Increasing pH, DOM, or carboxyl modification of the NP surface also improved NP stability in solution. Increased salinity of the solution caused aggregation of unmodified PS independent of chitosan, but carboxyl-modified PS remained stable and aggregated at the same chitosan doses across all salinity levels. Chitosan with low molecular weight promoted PS aggregation at lower doses. Notably, zeta potential (ZP) alone did not indicate chitosan-induced PS aggregation, which occurred independently of changes in ZP. DLVO calculations based on ZP, however, still indicated attractive interaction due to charge differences, albeit with less contrast at high pH, salinity, and DOM content. Additional insights gained in the work recommend caution when using spectrophotometric methods to assess NP removal. Overall, this study demonstrates that chitosan impacts NP transport and holds potential for water remediation of NP., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Microplastic analysis requires effective separation and purification methods, which greatly depend on the matrix and target particle size. Microplastics-sediment extraction usually involves intermediate steps, increasing processing time and particle loss, particularly for particles <100 μm. Here, we propose an improved separation and quantification method for fine-grained sediment that minimizes microplastic loss by reducing intermediate steps. First, the sample is treated with CH 3 COOH, KOH and NaClO, and only transferred for the density separation (ZnCl 2 ). The extraction efficiency, visually evaluated on spiked samples, was higher than 90% for particles >100 μm and 83% for 63-75 μm particles. This indicates that a sequential extraction method reduces the risk of particle loss, particularly of the small size fraction. Comparatively, the extraction of ABS particles (20-100 μm) was low (30%) but the recovery, assessed via μFTIR, was higher (55%). Additionally, the proposed method can be adapted to other sediment types and environmental matrices., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Rivers and estuaries are regarded as major pathways of microplastic (MP) transport from terrestrial areas to marine ecosystems. Despite this knowledge on the transport dynamics and fate of MP in freshwater riverine and brackish estuarine waters is limited. Via ex situ settling experiments emulating the Msimbazi River and Estuary in Dar es-Salaam, Tanzania, we demonstrate that flocculation and subsequent settling of positively buoyant MP and fine-grained suspended sediment in riverine and estuarine waters are important for the environmental fate of the plastic particles. Our results show that settling velocities of MP and fine-grained sediment in estuarine water were between five and 21 times larger than in freshwater, explained by the increase in ionic strength that occurs when particles enter saline water. This confirms the concept of increased flocculation and settling of fine-grained particles as they are transported from freshwater to estuarine and marine waters. The implication is that land-based sources of small positively buoyant high-density polyethylene (HDPE) MP transported by rivers will tend to settle and accumulate in estuarine environments and thereby lead to a decrease in the overall load of MPs delivered to the wider marine environment. Thereby our results support the notion of estuaries as MP traps and that flocculation explains the trapping of large quantities of MP debris. Based on these findings we recommend that the interaction of MP with fine-grained sediment should be taken into account when transport models of this pollutant are established., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Viet Nam is challenged by extensive marine plastic pollution, however, remediation efforts are hampered by undefined sources to the coastal environment. This study surveyed the abundance, type, and source of beached plastic litter at seven beaches along the coast of Nha Trang, Viet Nam. A total of 4754 beached plastic litter items (>2 cm) yielded a mean abundance of 19.8 ± 19.5 items m -2 corresponding to 116 ± 226 g DW m -2 . Our results demonstrate that plastic litter related to fishing and aquaculture constituted at least 62 % of the total by weight and 38 % by number, showing that these two sectors are responsible for a significant part of the plastic pollution along the coast. Hence, we argue that improved management of the fishing and aquaculture sectors could substantially reduce marine plastic pollution along Viet Nam's coast., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Blooms of purple sulfur bacteria (PSB) are important drivers of the global sulfur cycling oxidizing reduced sulfur in intertidal flats and stagnant water bodies. Since the discovery of PSB Chromatium okenii in 1838, it has been found that this species is characteristic of for stratified, sulfidic environments worldwide and its autotrophic metabolism has been studied in depth since. We describe here the first high-quality draft genome of a large-celled, phototrophic, γ-proteobacteria of the genus Chromatium isolated from the stratified alpine Lake Cadagno, C. okenii strain LaCa. Long read technology was used to assemble the 3.78 Mb genome that encodes 3,016 protein-coding genes and 67 RNA genes. Our findings are discussed from an ecological perspective related to Lake Cadagno. Moreover, findings of previous studies on the phototrophic and the proposed chemoautotrophic metabolism of C. okenii were confirmed on a genomic level. We additionally compared the C. okenii genome with other genomes of sequenced, phototrophic sulfur bacteria from the same environment. We found that biological functions involved in chemotaxis, movement and S-layer-proteins were enriched in strain LaCa. We describe these features as possible adaptions of strain LaCa to rapidly changing environmental conditions within the chemocline and the protection against phage infection during blooms. The high quality draft genome of C. okenii strain LaCa thereby provides a basis for future functional research on bioconvection and phage infection dynamics of blooming PSB.

The toxicity of arsenic (As) towards life on Earth is apparent in the dense distribution of genes associated with As detoxification across the tree of life. The ability to defend against As is particularly vital for survival in As-rich shallow submarine hydrothermal ecosystems along the Hellenic Volcanic Arc (HVA), where life is exposed to hydrothermal fluids containing up to 3000 times more As than present in seawater. We propose that the removal of dissolved As and phosphorus (P) by sulfide and Fe(III)(oxyhydr)oxide minerals during sediment-seawater interaction, produces nutrient-deficient porewaters containing < 2.0 ppb P. The porewater arsenite-As(III) to arsenate-As(V) ratios, combined with sulfide concentration in the sediment and/or porewater, suggest a hydrothermally-induced seafloor redox gradient. This gradient overlaps with changing high affinity phosphate uptake gene abundance. High affinity phosphate uptake and As cycling genes are depleted in the sulfide-rich settings, relative to the more oxidizing habitats where mainly Fe(III)(oxyhydr)oxides are precipitated. In addition, a habitat-wide low As-respiring and As-oxidizing gene content relative to As resistance gene richness, suggests that As detoxification is prioritized over metabolic As cycling in the sediments. Collectively, the data point to redox control on Fe and S mineralization as a decisive factor in the regulation of high affinity phosphate uptake and As cycling gene content in shallow submarine hydrothermal ecosystems along the HVA.

Anoxygenic phototrophic bacteria utilize ancient metabolic pathways to link sulfur and iron metabolism to the reduction of CO 2 . In meromictic Lake Cadagno, Switzerland, both purple sulfur (PSB) and green sulfur anoxygenic phototrophic bacteria (GSB) dominate the chemocline community and drive the sulfur cycle. PSB and GSB fix carbon utilizing different enzymatic pathways and these fractionate C-isotopes to different extents. Here, these differences in C-isotope fractionation are used to constrain the relative input of various anoxygenic phototrophs to the bulk community C-isotope signal in the chemocline. We sought to determine whether a distinct isotopic signature of GSB and PSB in the chemocline persists in the settling fraction and in the sediment. To answer these questions, we also sought investigated C-isotope fractionation in the water column, settling material, and sediment of Lake Cadagno, compared these values to C-isotope fractionation of isolated anoxygenic phototroph cultures, and took a mass balance approach to investigate relative contributions to the bulk fractionation signature. We found a large C-isotope fractionation between dissolved inorganic carbon (DIC) and particulate organic carbon (POC) in the Lake Cadagno chemocline. This large fractionation between the DIC and POC was also found in culture experiments carried out with anoxygenic phototrophic bacteria isolated from the lake. In the Lake Cadagno chemocline, anoxygenic phototrophic bacteria controlled the bulk C-isotope fractionation, but the influence of GSB and PSB differed with season. Furthermore, the contribution of PSB and GSB to bulk C-isotope fractionation in the chemocline could be traced in the settling fraction and in the sediment. Taken together with other studies, such as lipid biomarker analyzes and investigations of other stratified lakes, these results offer a firmer understanding of diagenetic influences on bacterial biomass., (© 2017 John Wiley & Sons Ltd.)

The seafloor sediments of Spathi Bay, Milos Island, Greece, are part of the largest arsenic-CO 2 -rich shallow submarine hydrothermal ecosystem on Earth. Here, white and brown deposits cap chemically distinct sediments with varying hydrothermal influence. All sediments contain abundant genes for autotrophic carbon fixation used in the Calvin-Benson-Bassham (CBB) and reverse tricaboxylic acid (rTCA) cycles. Both forms of RuBisCO, together with ATP citrate lyase genes in the rTCA cycle, increase with distance from the active hydrothermal centres and decrease with sediment depth. Clustering of RuBisCO Form II with a highly prevalent Zetaproteobacteria 16S rRNA gene density infers that iron-oxidizing bacteria contribute significantly to the sediment CBB cycle gene content. Three clusters form from different microbial guilds, each one encompassing one gene involved in CO 2 fixation, aside from sulfate reduction. Our study suggests that the microbially mediated CBB cycle drives carbon fixation in the Spathi Bay sediments that are characterized by diffuse hydrothermal activity, high CO 2 , As emissions and chemically reduced fluids. This study highlights the breadth of conditions influencing the biogeochemistry in shallow CO 2 -rich hydrothermal systems and the importance of coupling highly specific process indicators to elucidate the complexity of carbon cycling in these ecosystems.

Millions of people worldwide are affected by As (arsenic) contaminated groundwater. Fe(III) (oxy)hydroxides sorb As efficiently and are therefore used in water purification filters. Commercial filters containing abiogenic Fe(III) (oxy)hydroxides (GEH) showed varying As removal, and it was unclear whether Fe(II)-oxidizing bacteria influenced filter efficiency. We found up to 10(7) Fe(II)-oxidizing bacteria/g dry-weight in GEH-filters and determined the performance of filter material in the presence and absence of Fe(II)-oxidizing bacteria. GEH-material sorbed 1.7 mmol As(V)/g Fe and was ~8 times more efficient than biogenic Fe(III) minerals that sorbed only 208.3 μmol As(V)/g Fe. This was also ~5 times more efficient than a 10:1-mixture of GEH-material and biogenic Fe(III) minerals that bound 322.6 μmol As(V)/g Fe. Coprecipitation of As(V) with biogenic Fe(III) minerals removed 343.0 μmol As(V)/g Fe, while As removal by coprecipitation with biogenic minerals in the presence of GEH-material was slightly less efficient as GEH-material only and yielded 1.5 mmol As(V)/g Fe. The present study thus suggests that the formation of biogenic Fe(III) minerals lowers rather than increases As removal efficiency of the filters probably due to the repulsion of the negatively charged arsenate by the negatively charged biogenic minerals. For this reason we recommend excluding microorganisms from filters (e.g., by activated carbon filters) to maintain their high As removal capacity.

Phototrophic iron(II) [Fe(II)]-oxidizing bacteria are present in modern environments and evidence suggests that this metabolism was present already on early earth. We determined Fe(II) oxidation rates depending on pH, temperature, light intensity, and Fe(II) concentration for three phylogenetically different phototrophic Fe(II)-oxidizing strains (purple nonsulfur bacterium Rhodobacter ferrooxidans sp. strain SW2, purple sulfur bacterium Thiodictyon sp. strain F4, and green sulfur bacterium Chlorobium ferrooxidans strain KoFox). While we found the overall highest Fe(II) oxidation rates with strain F4 (4.5 mmol L(-1) day(-1), 800 lux, 20 degrees C), the lowest light saturation values [at which maximum Fe(II) oxidation occurred] were determined for strain KoFox with light saturation already below 50 lux. The oxidation rate per cell was determined for R. ferrooxidans strain SW2 to be 32 pmol Fe(II) h(-1) per cell. No significant toxic effect of Fe(II) was observed at Fe(II) concentrations of up to 30 mM. All three strains are mesophiles with upper temperature limits of c. 30 degrees C. The main pigments were identified to be spheroidene, spheroidenone, OH-spheroidenone (SW2), rhodopinal (F4), and chlorobactene (KoFox). This study will improve our ecophysiological understanding of iron cycling in modern environments and will help to evaluate whether phototrophic iron oxidizers may have contributed to the formation of Fe(III) on early earth.