Search

Your search keyword '"Possel, Ronald"' showing total 25 results
Start Over Author "Possel, Ronald"
25 results on '"Possel, Ronald"'

3. Performance Analysis of Serodiagnostic Tests to Characterize the Incline and Decline of the Individual Humoral Immune Response in COVID-19 Patients: Impact on Diagnostic Management

Author

von Possel, Ronald, primary, Menge, Babett, additional, Deschermeier, Christina, additional, Fritzsche, Carlos, additional, Hemmer, Christoph, additional, Geerdes-Fenge, Hilte, additional, Loebermann, Micha, additional, Schulz, Anette, additional, Lattwein, Erik, additional, Steinhagen, Katja, additional, Tönnies, Ralf, additional, Ahrendt, Reiner, additional, and Emmerich, Petra, additional

Published
2024
Full Text
View/download PDF

4. Crimean-Congo Hemorrhagic Fever, Kosovo, 2013-2016

Author

Ahmeti, Salih, Berisha, Lindita, Halili, Bahrije, Ahmeti, Florim, von Possel, Ronald, Thome-Bolduan, Corinna, Michel, Anett, Priesnitz, Simone, Reisinger, Emil C., Gunther, Stephan, Kruger, Andreas, Sherifi, Kurtesh, Jakupi, Xhevat, Hemmer, Christoph J., and Emmerich, Petra

Subjects
Analysis, Research, Patient outcomes, Public health -- Analysis, Crimean hemorrhagic fever -- Research -- Patient outcomes, Epidemiology -- Analysis, Hemorrhagic fevers, Ribavirin, Medical research
Abstract

Crimean-Congo hemorrhagic fever (CCHF), caused by an orthonairovirus of the Nairoviridae family, is usually transmitted by bites of Hyalomma sp. ticks. Case-fatality rates vary from 10% to 40%. Most cases [...]

Published
2019
Full Text
View/download PDF

5. Serologic and Genomic Investigation of West Nile Virus in Kosovo.

Author

Emmerich, Petra, Jakupi, Xhevat, Sherifi, Kurtesh, Dreshaj, Shemsedin, Kalaveshi, Ariana, Hemmer, Christoph, Hajdari, Donjeta Pllana, von Possel, Ronald, Cadar, Dániel, and Tomazatos, Alexandru

Subjects
WEST Nile virus, WEST Nile fever, ARCHAEOLOGICAL human remains
Abstract

The prevalence of West Nile virus (WNV) is increasing across Europe, with cases emerging in previously unaffected countries. Kosovo is situated in a WNV-endemic region where the seroepidemiological data on WNV in humans remains absent. To address this issue, we have conducted a seroepidemiological investigation of 453 randomly selected sera from a hospital in Kosovo, revealing a 1.55% anti-WNV IgG seroprevalence. Comparative and phylogeographic analyses of the WNV genomes obtained by sequencing archived samples from patients with West Nile fever indicate at least two recent and distinct introductions of WNV lineage 2 into Kosovo from neighboring countries. These findings confirm the eco-epidemiological status of WNV in southeast Europe, where long- and short-range dispersion of lineage 2 strains contributes to a wider circulation via central Europe. Our results suggest an increasing risk for WNV spreading in Kosovo, underscoring the need for an integrated national surveillance program targeting vectors and avian populations for early epidemic detection, as well as the screening of blood donors to gauge the impact of virus circulation on the human population. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. Severe mpox in an immunocompromised patient complicated by deep tissue infection: A case report

Author

Pfefferle, Susanne, Schweizer, Michaela, Hartmann, Kristin, Berger, Julia, Nörz, Dominik, Emmerich, Petra, von Possel, Ronald, Giersch, Katja, Pflüger, Lisa Sophie, Bernreuther, Christian, Glatzel, Markus, Krasemann, Susanne, Brehm, Thomas Theo, Schulze zur Wisch, Julian, Fischer, Nicole, Schmiedel, Stefan, Aepfelbacher, Martin, and Lütgehetmann, Marc

Published
2024
Full Text
View/download PDF

8. Fcγ-Receptor-Based Enzyme-Linked Immunosorbent Assays for Sensitive, Specific, and Persistent Detection of Anti-SARS-CoV-2 Nucleocapsid Protein IgG Antibodies in Human Sera

Author

Deschermeier, Christina, primary, Ehmen, Christa, additional, von Possel, Ronald, additional, Murawski, Carolin, additional, Rushton, Ben, additional, Amuasi, John, additional, Sarpong, Nimako, additional, Maiga-Ascofaré, Oumou, additional, Rakotozandrindrainy, Raphael, additional, Asogun, Danny, additional, Ighodalo, Yemisi, additional, Oestereich, Lisa, additional, Duraffour, Sophie, additional, Pahlmann, Meike, additional, and Emmerich, Petra, additional

Published
2022
Full Text
View/download PDF

9. Clinical efficacy and in vitro neutralization capacity of monoclonal antibodies for severe acute respiratory syndrome coronavirus 2 delta and omicron variants

Author

Brehm, Thomas Theo, primary, Pfefferle, Susanne, additional, von Possel, Ronald, additional, Karolyi, Mario, additional, Zoufaly, Alexander, additional, Wichmann, Dominic, additional, Kobbe, Robin, additional, Emmerich, Petra, additional, Nörz, Dominik, additional, Aepfelbacher, Martin, additional, Schulze zur Wiesch, Julian, additional, Addo, Marylyn M., additional, Schmiedel, Stefan, additional, and Lütgehetmann, Marc, additional

Published
2022
Full Text
View/download PDF

10. Cover Image, Volume 93, Number 10, October 2021

Author

Emmerich, Petra, primary, Possel, Ronald, additional, Hemmer, Christoph Josef, additional, Fritzsche, Carlos, additional, Geerdes‐Fenge, Hilte, additional, Menge, Babett, additional, Messing, Claudia, additional, Borchardt‐Lohölter, Viola, additional, Deschermeier, Christina, additional, and Steinhagen, Katja, additional

Published
2021
Full Text
View/download PDF

12. SARS-CoV-2 Reinfection in a Healthcare Worker Despite the Presence of Detectable Neutralizing Antibodies

Author

Brehm, Thomas Theo, primary, Pfefferle, Susanne, additional, von Possel, Ronald, additional, Kobbe, Robin, additional, Nörz, Dominik, additional, Schmiedel, Stefan, additional, Grundhoff, Adam, additional, Olearo, Flaminia, additional, Emmerich, Petra, additional, Robitaille, Alexis, additional, Günther, Thomas, additional, Braun, Platon, additional, Andersen, Gabriele, additional, Knobloch, Johannes K., additional, Addo, Marylyn M., additional, Lohse, Ansgar W., additional, Aepfelbacher, Martin, additional, Fischer, Nicole, additional, Schulze zur Wiesch, Julian, additional, and Lütgehetmann, Marc, additional

Published
2021
Full Text
View/download PDF

13. Limited specificity of commercially available SARS‐CoV‐2 IgG ELISAs in serum samples of African origin

Author

Emmerich, Petra, primary, Murawski, Carolin, additional, Ehmen, Christa, additional, Possel, Ronald, additional, Pekarek, Neele, additional, Oestereich, Lisa, additional, Duraffour, Sophie, additional, Pahlmann, Meike, additional, Struck, Nicole, additional, Eibach, Daniel, additional, Krumkamp, Ralf, additional, Amuasi, John, additional, Maiga‐Ascofaré, Oumou, additional, Rakotozandrindrainy, Raphael, additional, Asogun, Danny, additional, Ighodalo, Yemisi, additional, Kann, Simone, additional, May, Jürgen, additional, Tannich, Egbert, additional, and Deschermeier, Christina, additional

Published
2021
Full Text
View/download PDF

14. Comparison of diagnostic performances of ten different immunoassays detecting anti-CCHFV IgM and IgG antibodies from acute to subsided phases of Crimean-Congo hemorrhagic fever

Author

Emmerich, Petra, primary, von Possel, Ronald, additional, Deschermeier, Christina, additional, Ahmeti, Salih, additional, Berisha, Lindita, additional, Halili, Bahrije, additional, Jakupi, Xhevat, additional, Sherifi, Kurtesh, additional, Messing, Claudia, additional, and Borchardt-Lohölter, Viola, additional

Published
2021
Full Text
View/download PDF

16. Limited specificity of commercially available SARS-CoV-2 IgG ELISAs in serum samples of African origin

Author

Emmerich, Petra, primary, Murawski, Carolin, additional, Possel, Ronald von, additional, Oestereich, Lisa, additional, Duraffour, Sophie, additional, Pahlmann, Meike, additional, Struck, Nicole, additional, Eibach, Daniel, additional, Krumkamp, Ralf, additional, Amuasi, John, additional, Maiga-Ascofare, Oumou, additional, Rakotozandrindrainy, Raphael, additional, Asogun, Danny, additional, Ighodalo, Yemisi, additional, May, Jürgen, additional, Tannich, Egbert, additional, and Deschermeier, Christina, additional

Published
2020
Full Text
View/download PDF

17. Mütter-Screening in einem COVID-19-Niedrig-Pandemiegebiet: Bestimmung SARS-CoV-2-spezifischer Antikörper bei 401 Rostocker Müttern mittels ELISA und Immunfluoreszenz-Bestätigungstest

Author

Reisinger, Emil C., additional, von Possel, Ronald, additional, Warnke, Philipp, additional, Geerdes-Fenge, Hilte F., additional, Hemmer, Christoph J., additional, Pfefferle, Susanne, additional, Löbermann, Micha, additional, Littmann, Martina, additional, and Emmerich, Petra, additional

Published
2020
Full Text
View/download PDF

20. Immunoglobulin-like Domain of HsFcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies

Author

Rackow, Anne, primary, Ehmen, Christa, primary, von Possel, Ronald, primary, Medialdea-Carrera, Raquel, primary, Brown, David, primary, Bispo de Filippis, Ana Maria, primary, Carvalho de Sequeira, Patrícia, primary, Ribeiro Nogueira, Rita Maria, primary, Halili, Barie, primary, Jakupi, Xhevat, primary, Berisha, Lindita, primary, Ahmeti, Salih, primary, Sherifi, Kurtesh, primary, Schmidt-Chanasit, Jonas, primary, Schmitz, Herbert, primary, Mika, Angela, primary, Emmerich, Petra, primary, and Deschermeier, Christina, primary

Published
2019
Full Text
View/download PDF

21. Sensitive and specific detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)—Specific IgM and IgG antibodies in human sera using recombinant CCHFV nucleoprotein as antigen in μ-capture and IgG immune complex (IC) ELISA tests

Author

Emmerich, Petra, primary, Mika, Angela, additional, von Possel, Ronald, additional, Rackow, Anne, additional, Liu, Yang, additional, Schmitz, Herbert, additional, Günther, Stephan, additional, Sherifi, Kurtesh, additional, Halili, Barie, additional, Jakupi, Xhevat, additional, Berisha, Lindita, additional, Ahmeti, Salih, additional, and Deschermeier, Christina, additional

Published
2018
Full Text
View/download PDF

22. Immunoglobulin-like Domain of HsFcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies.

Author

Ehmen, Christa, von Possel, Ronald, Medialdea-Carrera, Raquel, Brown, David, de Filippis, Ana Maria Bispo, de Sequeira, Patrícia Carvalho, Nogueira, Rita Maria Ribeiro, Halili, Barie, Jakupi, Xhevat, Berisha, Lindita, Ahmeti, Salih, Sherifi, Kurtesh, Schmidt-Chanasit, Jonas, Schmitz, Herbert, Mika, Angela, Emmerich, Petra, and Deschermeier, Christina

Published
2019
Full Text
View/download PDF

23. Genomic characterization of Orthonairovirus haemorrhagiae (Crimean-Congo hemorrhagic fever virus) outbreak in North Macedonia.

Author

Boshevska G, Emmerich P, von Possel R, Jancheska E, Buzharova T, Kochinski D, Tóth GE, Cadar D, and Osmani D

Abstract

Crimean-Congo hemorrhagic fever virus (CCHFV) is a significant tick-borne virus causing severe hemorrhagic disease with high fatality rate. This report presents the genomic characterization of CCHFV strain from North Macedonia's first outbreak in over 50 years, emphasizing the importance of genomic surveillance in tracking virus evolution and spread patterns in this region., Competing Interests: The authors declare no conflict of interest.

Published
2024
Full Text
View/download PDF

24. [Screening of Mothers in a COVID-19 Low-Prevalence Region: Determination of SARS-CoV-2 Antibodies in 401 Mothers from Rostock by ELISA and Confirmation by Immunofluorescence].

Author

Reisinger EC, von Possel R, Warnke P, Geerdes-Fenge HF, Hemmer CJ, Pfefferle S, Löbermann M, Littmann M, and Emmerich P

Subjects
Adolescent, Adult, Aged, Aged, 80 and over, Betacoronavirus genetics, Betacoronavirus isolation & purification, COVID-19, Child, Child, Preschool, Coronavirus Infections diagnosis, Coronavirus Infections immunology, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Germany epidemiology, Humans, Infant, Male, Mass Screening methods, Middle Aged, Oropharynx virology, Pandemics, Pneumonia, Viral diagnosis, Pneumonia, Viral immunology, Polymerase Chain Reaction, Prevalence, RNA, Viral analysis, SARS-CoV-2, Young Adult, Antibodies, Viral blood, Betacoronavirus immunology, Coronavirus Infections epidemiology, Pneumonia, Viral epidemiology
Abstract

Background: In children, the infection with SARS-CoV-2, the cause of COVID-19, tends to be clinically inapparent more often or less severe than in adults. The spread of this infection from children poses a danger to vulnerable adults. Therefore, child care institutions and schools currently are widely closed., Methods: Since the status of infection tends to be congruent in mothers and their children, we tested 401 mothers of children between 1 and 10 years in the city of Rostock (State of Mecklenburg-Westpomerania, northeast of Germany), for the presence of RNA of SARS-CoV-2 in throat swabs, and of antibodies against SARS-CoV-2 in serum., Results: In none of the mothers tested, RNA of this virus was detected in the throat swab. In the ELISA test, IgG antibodies were positive in one serum sample, IgA antibodies were positive in 11, and borderline in 3 serum samples. All 401 sera were negative in the indirect immunofluorescence test (IIFT) with FITC-labeled IgG, IgA, und IgM antibodies., Conclusion: At the time of this study, neither SARS-CoV-2 RNA, nor specific antibodies against SARS-CoV-2 were detectable in the mothers tested in Rostock., Competing Interests: Die Autorinnen/Autoren geben an, dass kein Interessenkonflikt besteht., (© Georg Thieme Verlag KG Stuttgart · New York.)

Published
2020
Full Text
View/download PDF

25. Immunoglobulin-like Domain of Hs FcμR as a Capture Molecule for Detection of Crimean-Congo Hemorrhagic Fever Virus- and Zika Virus-Specific IgM Antibodies.

Author

Rackow A, Ehmen C, von Possel R, Medialdea-Carrera R, Brown D, Bispo de Filippis AM, Carvalho de Sequeira P, Ribeiro Nogueira RM, Halili B, Jakupi X, Berisha L, Ahmeti S, Sherifi K, Schmidt-Chanasit J, Schmitz H, Mika A, Emmerich P, and Deschermeier C

Subjects
Animals, Antibodies, Viral metabolism, Apoptosis Regulatory Proteins metabolism, Base Sequence, Enzyme-Linked Immunosorbent Assay methods, Hemorrhagic Fever, Crimean diagnosis, Humans, Immunoglobulin Domains, Immunoglobulin M metabolism, Membrane Proteins metabolism, Protein Binding, Serologic Tests methods, Zika Virus Infection diagnosis, Antibodies, Viral blood, Hemorrhagic Fever Virus, Crimean-Congo immunology, Immunoglobulin M blood, Zika Virus immunology
Abstract

Background: The cellular surface molecule Hs TOSO/FAIM3/ Hs FcμR has been identified as an IgM-specific Fc receptor expressed on lymphocytes. Here, we show that its extracellular immunoglobulin-like domain ( Hs FcμR-Igl) specifically binds to IgM/antigen immune complexes (ICs) and exploit this property for the development of novel detection systems for IgM antibodies directed against Crimean-Congo hemorrhagic fever virus (CCHFV) and Zika virus (ZIKV)., Methods: His-tagged Hs FcμR-Igl was expressed in Escherichia coli and purified by affinity chromatography, oxidative refolding, and size-exclusion chromatography. Specific binding of Hs FcμR-Igl to IgM/antigen ICs was confirmed, and 2 prototypic ELISAs for the detection of anti-CCHFV and anti-ZIKV IgM antibodies were developed. Thereby, patient sera and virus-specific recombinant antigens directly labeled with horseradish peroxidase (HRP) were coincubated on Hs FcμR-Igl-coated ELISA plates. Bound ICs were quantified by measuring turnover of a chromogenic HRP substrate., Results: Assay validation was performed using paired serum samples from 15 Kosovar patients with a PCR-confirmed CCHFV infection and 28 Brazilian patients with a PCR-confirmed ZIKV infection, along with a panel of a priori CCHFV/ZIKV-IgM-negative serum samples. Both ELISAs were highly reproducible. Sensitivity and specificity were comparable with or even exceeded in-house gold standard testing and commercial kits. Furthermore, latex beads coated with Hs FcμR-Igl aggregated upon coincubation with an IgM-positive serum and HRP-labeled antigen but not with either component alone, revealing a potential for use of Hs FcμR-Igl as a capture molecule in aggregation-based rapid tests., Conclusions: Recombinant Hs FcμR-Igl is a versatile capture molecule for IgM/antigen ICs of human and animal origin and can be applied for the development of both plate- and bead-based serological tests., (© 2018 American Association for Clinical Chemistry.)

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results