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This study aimed to assess the presence of ochratoxin A (OTA) residues in the blood serum of slaughtered pigs in Greece. Samples were obtained from 1695 healthy slaughtered pigs originating from 113 different farms located in 21 geographic regional units in 8 different geographic regions of Greece and were analyzed using an immunosorbent assay (ELISA) and high-performance liquid chromatography with fluorescence detector (HPLC-FD). OTA contamination assessment showed that 782 (46.1%) and 1233 (72.7%) samples were OTA-positive, with a concentration range of 0.20–5.38 μg/L and 0.15–5.96 µg/L according to ELISA and HPLC-FD analysis, respectively. Also, 88 (77.9%) and 108 (95.6%) of farms were found to be OTA-positive by ELISA and HPLC-FD analysis, respectively. The highest OTA serum positivity rate (>98%) and toxin level (5.96 µg/L) determined by HPLC-FD were observed in the Thessaly region, whereas a high prevalence of up to 100% (range 75–100%) was found on farms in the Crete Island region. The detection of OTA in the serum of slaughtered pigs in different regions in Greece poses a risk for animal and human health and highlights the need for constant OTA monitoring in the swine industry and pork meat production facilities. [ABSTRACT FROM AUTHOR]

Simple Summary: Due to the serious problems caused by Escherichia coli (E. coli) in pigs, it is essential to prevent post-weaning diarrhea and the subsequent growth delay that this entails. In this study, we evaluated intestinal integrity, immune activation and morphometry and cellular composition at the tissue level in different sections of intestine in piglets subjected to oral E. coli vaccination in the middle and long term after the vaccine challenge. The results obtained show greater immune stimulation and intestinal integrity in the vaccinated animals compared to the control group with an increase in gene expression in most of the cytokines studied, better parameters in histomorphometry as well as a greater density of intraepithelial lymphocytes. Post-weaning stress, together with Escherichia coli, are two of the key factors in the occurrence of post-weaning diarrhea. There are different commercial vaccines that induce immunity at the local or systemic level, improving farm health and avoiding economic losses in the pork industry. That is why the objective of this study was to evaluate the effect of an oral enterotoxigenic E. coli F4/F18 vaccine on immunity and intestinal integrity in the middle and long term after inoculation. The gene expression of the biomarkers indicative of cellular infiltration (calprotectin, CAL), tight junction proteins (occludin, OCL; zonulin, ZON; and claudin, CLA) and a panel of proinflammatory (interleukins, IL: IL1α, IL1β, IL6, IL8, IL12p35 and IL12p40; interferons, IFN: IFNα and IFNγ; and tumoral necrosis factor, TNF: TNFα) and anti-inflammatory mediator cytokines (TGFβ and IL10) were analyzed, as well as histomorphology in jejunum and ileum, the cell density of goblet cells, intraepithelial lymphocytes and IgA-producing cells. Differences were observed in ZON, CLA and CAL, with greater gene expression in observed in vaccinated piglets at 42 days post vaccination (dpv) in the ileum. Regarding the expression of cytokines, the vaccinated animals showed significant differences in IL1α, IL6, IL12p35, IL12p40, IFNα, IFNγ, TNFα and TGFβ at 42 dpv in the jejunum or ileum. The villi showed greater height in the vaccinated piglets and the ratio between villus height and crypt depth was significantly greater in the vaccinated group in the jejunum at 84 dpv. The count of IgA-producing cells shows higher values for the unvaccinated group in the ileum, while intraepithelial lymphocytes show a significant increase in both jejunum and ileum in vaccinated piglets. We can conclude that oral vaccination against E. coli produces an evident effect, which manifests itself even in the middle and long term after the challenge, including immune response, decrease in antimicrobials usage, better histological structure in intestine and the improvement of performance. [ABSTRACT FROM AUTHOR]

Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV) infection, has been a serious threat to the pork industry worldwide and continues to bring significant economic loss. Current vaccination strategies offer limited protection against PRRSV transmission, highlighting the urgent need for novel antiviral approaches. In the present study, we reported for the first time that betulonic acid (BA), a widely available pentacyclic triterpenoids throughout the plant kingdom, exhibited potent inhibition on PRRSV infections in both Marc-145 cells and primary porcine alveolar macrophages (PAMs), with IC50 values ranging from 3.3 µM to 3.7 µM against three different type-2 PRRSV strains. Mechanistically, we showed that PRRSV replication relies on energy supply from cellular ATP production, and BA inhibits PRRSV infection by reducing cellular ATP production. Our findings indicate that controlling host ATP production could be a potential strategy to combat PRRSV infections, and that BA might be a promising therapeutic agent against PRRSV epidemics. [ABSTRACT FROM AUTHOR]

Context: Over the past four decades, major changes have occurred in Australia's pork industry, affecting productivity and environmental performance. Aims: This study determined long-term changes in greenhouse gas and key resource use efficiency indicators. Methods: Life cycle assessment was used to determine impacts at decadal intervals between 1980 and 2010, and are presented alongside results for 2020 and 2022. Key results: Over 42 years since 1980, greenhouse gas emissions, excluding land use and direct land use change (dLUC), fell by 74% from 11.7 to 3.0 kg CO2-e/kg liveweight. Land use and dLUC emissions declined by 92%. Fossil energy use decreased from 35 to 13 MJ/kg liveweight between 1980 and 2022. Freshwater consumption and water stress fell from 506 L and 671 L H2O-e in 1980 to 52 L and 43 L H2O-e/kg liveweight in 2022, respectively. Land occupation decreased by 42% from 22 m2/kg liveweight in 1980 to 13 m2/kg liveweight in 2022. Over the analysis period, emissions per kilogram of liveweight fell by an average of 1.8% per year, land use and dLUC emissions by 2.2%, greenhouse gas including land use and dLUC emissions by 1.9%, fossil energy use by 1.5%, and freshwater consumption, stress, and land occupation by 2.1%, 2.2%, and 1%, respectively. Between 2010 and 2020, uptake of covered anaerobic ponds resulted in an annual rate of improvement in emissions (excl. land use and dLUC) of 2.9%, however, the rate of improvement fell to 1.4% between 2020 and 2022. Conclusions: Long-term improvements were principally driven by improved herd productivity and feed production systems, and changes in housing and manure management. Herd and system efficiencies led to better feed conversion ratio, resulting in lower feed requirements, reduced manure production and lower feed wastage, which reduced manure greenhouse gas emissions. Concurrently, reduced tillage, higher yields, and a decrease in the proportion of irrigation water used for grain production resulted in lower impacts of feed grains. Implications: Ongoing changes and improvements in production efficiency have resulted in large gains in environmental performance in the Australian pork industry but new strategies will also be needed to maintain these trends into the future. Long-term production efficiency gains and changes to production have significantly improved the environmental performance of Australian pork over time. This study was a long-term trends analysis of Australian pork between 1980 and 2022 and found that greenhouse gas emissions and freshwater consumption fell by 74% and 90% over the analysis period. Although novel low carbon production systems were identified, the rate of environmental improvement was found to have slowed since 2010. [ABSTRACT FROM AUTHOR]

Purpose: A meat quality grading system is essential to meet consumers' increasingly diversified demand for food quality in the global market. This study aims to determine the effectiveness of the upcoming Chinese quality grading labels and examine the information effect of labeling standards on pork consumption choices. Design/methodology/approach: Using an online survey with choice experiments, this study estimates consumer valuation for the fat thickness of different pork primal cuts by simulating three scenarios. Generalized mixed logit models in WTP space are used to analyze the choice experiment data. Findings: Chinese consumers prefer lean pork to fatty pork and this preference does not vary significantly between primal cuts. Consumer valuation for ungraded high-quality (lean) pork increases after the implementation of the quality grading. Meanwhile, they are willing to pay high premiums for labeled pork (including level 1, 2, 3), and there are higher premiums for pork with higher levels. Besides, incomplete information on labeling standards could achieve more premiums for pork than relatively complete information. Originality/value: This study pays attention to essential but few-noticed pork quality grading. The findings provide references for pork industry practices and policy-making of the meat quality grading system in China and globally by examining incomplete and relatively complete information effects on consumer choices. [ABSTRACT FROM AUTHOR]

Viruses deploy sophisticated strategies to hijack the host’s translation machinery to favor viral protein synthesis and counteract innate cellular defenses. However, little is known about the mechanisms by which Senecavirus A (SVA) controls the host’s translation. Using a series of sophisticated molecular cell manipulation techniques, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) was identified as an essential host factor involved in translation control in SVA-infected cells. It was also determined that the SVA structural protein, VP3, binds to and relocalizes hnRNPA2B1, which interferes with the host’s protein synthesis machinery to establish a cellular environment that facilitates viral propagation via a two-pronged strategy: first, hnRNPA2B1 serves as a potent internal ribosome entry site (IRES) trans-acting factor, which is selectively co-opted to promote viral IRES-driven translation by supporting the assembly of translation initiation complexes. Second, a strong repression of host cell translation occurs in the context of the VP3-hnRNPA2B1 interaction, resulting in attenuation of the interferons response. This is the first study to demonstrate the interaction between SVA VP3 and hnRNPA2B1, and to characterize their key roles in manipulating translation. This novel dual mechanism, which regulates selective mRNA translation and immune evasion of virus-infected cells, highlights the VP3-hnRNPA2B1 complex as a potential target for the development of modified antiviral or oncolytic reagents. IMPORTANCE Viral reproduction is contingent on viral protein synthesis, which relies entirely on the host's translation machinery. As such, viruses often need to control the cellular translational apparatus to favor viral protein production and avoid host innate defenses. Senecavirus A (SVA) is an important virus, both as an emerging pathogen in the pork industry and as a potential oncolytic virus for neuroendocrine cancers. Here, heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) was identified as a critical regulator of the translational landscape during SVA infection. This study supports a model whereby the VP3 protein of SVA efficiently subverts the host’s protein synthesis machinery through its ability to bind to and relocalize hnRNPA2B1, not only selectively promoting viral internal ribosome entry site-driven translation but also resulting in global translation shutdown and immune evasion. Together, these data provide new insights into how the complex interactions between translation machinery, SVA, and innate immunity contribute to the pathogenicity of the SVA. [ABSTRACT FROM AUTHOR]

Simple Summary: The rapidly growing wild boar population has resulted in an increasing rate of human–wildlife conflicts, including economic damage to crops or spreading African swine fever (ASF), which affects the pork industry. This situation necessitates the adoption of various measures to prevent wild boar movement, mitigate spreading of diseases, and protect crops. We evaluated the impact of commonly used odor fences (Wildschwein Stopp) using GPS telemetry of tagged individuals. The telemetry of free-ranging wild boars demonstrated no effect on their crossing of the odor fence lines compared to before the installation. Moreover, no difference was found when comparing the home range size of monitored individuals during the 22 days before and after odor fence installation. Therefore, our findings do not support using odor fences to prevent wild boar movement as a mitigation measure of ASF transmission or line protection against the damage caused by wild boars to crops. Wild boars are an opportunistic wildlife species that has successfully colonized the human-modified landscape in Europe. However, the current population boom has negative consequences, which result in a rapid increase in human–wildlife conflicts and disease transmission, including African swine fever (ASF). The increasing frequency of conflicts requires adequate solutions for these issues through various measures. Application of deterrents is a common non-lethal measure whose effects have been insufficiently verified until recently. Thus, this study aims to evaluate the effectiveness of odor fences, often applied as a barrier against wild boar movement. For this purpose, 18 wild boars were marked with GPS collars. After 22 days of initial monitoring, 12 sections of odor fences were installed on their home ranges. The monitored wild boars crossed the area 20.5 ± 9.2 times during the pre-installation period and 19.9 ± 8.4 times after the odor fence installation. Moreover, the average home range varied between 377.9 ± 185.0 ha before and 378.1 ± 142.2 ha after the odor fence installation. Based on GPS telemetry results, we do not support using odor repellent lines for crop protection or for limiting wild boar movement to lessen ASF outbreaks. [ABSTRACT FROM AUTHOR]

Simple Summary: African swine fever (ASF) presents a significant challenge in Asia, where outbreaks have devastated the pork industry and threatened food security due to high mortality rates in pigs. The disease, caused by the ASF virus genotype II, has spread rapidly across the region since its emergence in China in 2018. This paper discusses the introduction and implications of vaccines such as NAVET-ASFVAC and AVAC ASF Live in Vietnam, emphasizing the necessity for rigorous testing and regulatory oversight. Despite the potential use of vaccines to control the disease, concerns about the safety of live attenuated vaccines (LAVs), including their ability to revert to virulence or create new recombinant strains, highlight the complexity of ASF management. Effective vaccine strategies, alongside strict biosecurity measures, and rapid diagnostics are essential to mitigate the economic and social impacts of ASF and ensure the stability of pig populations in Asia. This paper explores the significance of quality vaccines in managing ASF in Asia, where it poses a substantial threat to the pork industry. It emphasizes the risks associated with substandard vaccines, including the emergence of new virus strains that complicate disease control. Highlighting recent advancements in vaccine deployment in Vietnam, the paper calls for rigorous testing and regulations to guarantee vaccine effectiveness and safety. The authors advocate for the implementation of vaccines with the inclusion of differentiating infected from vaccinated animals (DIVA), which enhances disease management strategies in both endemic and non-endemic regions. The conclusion underscores the necessity of stringent standards in vaccine development and strict adherence to regulatory guidelines to ensure successful ASF management and maintain public trust in the vaccines. [ABSTRACT FROM AUTHOR]

Boar semen production plays a pivotal role in modern swine breeding programmes, influencing the genetic progress and overall efficiency of the pork industry. This review explores the current challenges and emerging trends in liquid‐preserved boar semen production, addressing key issues that impact the quality and quantity of boar semen. Advances in new reproductive technologies, boar selection, housing, semen processing, storage and transport, and the need for sustainable practices including the use of artificial intelligence are discussed to provide a comprehensive overview of the field. [ABSTRACT FROM AUTHOR]

Is there a link between meat production and infectious diseases? Researchers, policymakers and pundits argue that the growth of demand for meat in Asia can increase the risk of zoonotic diseases, pathogens that originate in animals and can be transmitted to humans. Using original data on zoonotic disease outbreaks in 22 Asian countries between 1996 and 2019, this study examines the impact of beef, chicken and pork production on zoonotic outbreaks—focussing on all diseases and disaggregated subcategories. Because such outbreaks lead to culling animals, data on inorganic chemical fertiliser use are used to identify the plausibly exogenous relationship flowing from meat production to outbreaks. Results indicate that in these countries, intensive meat production may have contributed to a 16%–300% rise (for a one‐million‐tonne increase in output) in zoonotic outbreak incidence. Findings also suggest this relationship is primarily driven by flu pathogens' higher sensitivity to meat production, with a one‐million‐tonne increase in output being associated with a 48%–530% rise in the number of predicted flu outbreaks. These results are consistent across numerous sensitivity analyses accounting for modelling, operationalisation, and data selection choices. [ABSTRACT FROM AUTHOR]