10 results on '"Popovska-Jankovic K"'
Search Results
2. MicroRNAs in Breast Cancer — Our Initial Results
- Author
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Plaseska-Karanfilska Dijana, Popovska-Jankovic K, Noveski P, Chakalova L, Petrusevska G, and Kubelka K
- Subjects
breast cancer ,microrna (mirna) ,microarray ,real time-polymerase chain reaction (reti- pcr) ,stem-loop reverse transcriptase (rt) primers ,Genetics ,QH426-470 - Abstract
MicroRNAs (miRNAs) are small [~21 nucleotide (nt)] non coding RNAs (ncRNAs) that regulate gene expression posttranscriptionally. About 3.0% of human genes encode for miRNAs, and up to 30.0% of human protein coding genes may be regulated by miRNAs. Currently, more than 2000 unique human mature microRNAs are known. MicroRNAs play a key role in diverse biological processes including development, cell proliferation, differentiation and apoptosis. These processes are commonly dysregulated in cancer, implicating miRNAs in carcinogenesis, where they act as tumor supressors or oncogenes. Several miRNAs are associated with breast cancer. Here we present our initial results of miRNA analyses of breast cancer tissues using quantitative real time-polymerase chain reaction (ReTi-PCR) (qPCR) involving stem-loop reverse transcriptase (RT) primers combined with TaqMan® PCR and miRNA microarray analysis more...
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- 2012
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Catalog
3. MicroRNA expression profiles in testicular biopsies of patients with impaired spermatogenesis
- Author
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Noveski, P., primary, Popovska‐Jankovic, K., additional, Kubelka‐Sabit, K., additional, Filipovski, V., additional, Lazarevski, S., additional, Plaseski, T., additional, and Plaseska‐Karanfilska, D., additional more...
- Published
- 2016
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4. Cystinuria in south east European countries: mutations in SLC3A1 and SLC7A9 genes
- Author
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Bogdanovic, R., Plaseska-Karanfilska, D., Efremov, G., Topaloglu, R., Baskin, E., Akil, I., Soylu, A., Miljkovic, P., Tasic, V., and Popovska-Jankovic, K.
- Published
- 2006
5. TIMP3 Promoter Methylation Represents an Epigenetic Marker of BRCA1ness Breast Cancer Tumours.
- Author
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Maleva Kostovska I, Jakimovska M, Popovska-Jankovic K, Kubelka-Sabit K, Karagjozov M, and Plaseska-Karanfilska D
- Subjects
- Breast Neoplasms genetics, Breast Neoplasms pathology, Epigenesis, Genetic, Female, Humans, Promoter Regions, Genetic genetics, Triple Negative Breast Neoplasms pathology, BRCA1 Protein genetics, DNA Methylation genetics, Tissue Inhibitor of Metalloproteinase-3 genetics, Triple Negative Breast Neoplasms genetics
- Abstract
Tumours presenting BRCAness profile behave more aggressively and are more invasive as a consequence of their complex genetic and epigenetic alterations, caused by impaired fidelity of the DNA repair processes. Methylation of promoter CpG islands represents an alternative mechanism to inactivate DNA repair and tumour suppressor genes. In our study, we analyzed the frequency of methylation changes of 24 tumour suppressor genes and explored their association with BRCAness profile. BRCA1ness profile and aberrant methylation were studied in 233 fresh frozen breast tumour tissues by Multiplex Ligation-dependent Probe Amplification (MLPA) and Methylation Specific (MS)-MLPA methods, respectively. Our analyses revealed that 12.4% of the breast cancer (BC) patients had tumours with a BRCA1ness profile. TIMP3 showed significantly higher (p = 5.8х10
-5 ) methylation frequency in tumours with BRCA1ness, while methylation of APC, GSTP1 and RASSF1 promoters was negatively associated with BRCA1ness (р = 0.0017, р = 0.007 and р = 0.046, respectively). TIMP3 methylation was also associated with triple negative (TN) BC. Furthermore, TN tumours showing BRCA1ness showed stronger association with TIMP3 methylation (p = 0.0008) in comparison to TN tumours without BRCA1ness (p = 0.009). In conclusion, we confirmed that TIMP3 methylation is a marker for TN tumours and furthermore we showed for the first time that TIMP3 promoter methylation is an epigenetic marker of BRCA1ness tumours. more...- Published
- 2018
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6. BRCA1 and BRCA2 germline variants in breast cancer patients from the Republic of Macedonia.
- Author
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Jakimovska M, Maleva Kostovska I, Popovska-Jankovic K, Kubelka-Sabit K, Karadjozov M, Stojanovska L, Arsovski A, Smichkoska S, Lazarova E, Jakimovska Dimitrovska M, and Plaseska-Karanfilska D
- Subjects
- Adult, Breast Neoplasms epidemiology, Breast Neoplasms pathology, Female, Germ-Line Mutation genetics, Humans, Middle Aged, Republic of North Macedonia epidemiology, BRCA1 Protein genetics, BRCA2 Protein genetics, Breast Neoplasms genetics, Genetic Predisposition to Disease
- Abstract
Purpose: We aimed to establish the spectrum of BRCA1/2 mutations among the breast cancer (BC) patients from the Republic of Macedonia., Methods: We used targeted next-generation sequencing (NGS), Sanger DNA sequencing, and multiplex ligation probe amplification analysis (MLPA) to search for point mutations and deletions/duplications involving BRCA1 and BRCA2-coding regions., Results: We have analyzed a total of 313 BC patients, enriched for family history of cancer, early age of onset and bilateral and/or triple negative (TN) BC. A total of 26 pathogenic mutations were observed in 49 unrelated BC patients (49/313, 15.7%). BRCA2 mutations (27/49, 55.1%) were more common than BRCA1 mutations (22/49, 44.9%). We identified five novel point mutations, one in BRCA1 (c.4352_4356delA) and four in BRCA2 (c.151G>T, c.4707_4708delCA, c.7811_7814delTGTG, and c.9304_9305delG), as well as two novel deletions involving parts of the BRCA1 gene (c.81-?_593+?del and c.5470-?_5530+?del). The most common mutations were c.181T>G, c.5266dupC, and c.3700_3704del5 in BRCA1 and c.7879A>T, c.8317_8330del14 and c.5722_5723delCT in BRCA2 gene. Thus far, BRCA2 c.7879A>T and c.8317_8330del14 mutations have been described in several isolated cases; however, our study is the first one showing that they have a founder effect among Macedonian population. Nine recurrent mutations account for 65.3% of all of the detected mutations allowing for implementation of a fast first-step BRCA1/2 mutational screening strategy in our country., Conclusion: This study provides a comprehensive view of known and novel BRCA1/2 mutations in BC patients from the Republic of Macedonia and contributes to the global spectrum of BRCA1/2 mutations in breast cancer. more...
- Published
- 2018
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7. MicroRNA Profiling in Patients with Upper Tract Urothelial Carcinoma Associated with Balkan Endemic Nephropathy.
- Author
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Popovska-Jankovic K, Noveski P, Jankovic-Velickovic L, Stojnev S, Cukuranovic R, Stefanovic V, Toncheva D, Staneva R, Polenakovic M, and Plaseska-Karanfilska D
- Subjects
- Adult, Aged, Balkan Nephropathy epidemiology, Balkan Peninsula epidemiology, Carcinoma, Transitional Cell epidemiology, Female, Humans, Male, Middle Aged, Ureteral Neoplasms epidemiology, Balkan Nephropathy metabolism, Biomarkers, Tumor metabolism, Carcinoma, Transitional Cell metabolism, MicroRNAs metabolism, Ureteral Neoplasms metabolism
- Abstract
Balkan endemic nephropathy (BEN) is a disease that affects people that live in the alluvial plains along the tributaries of the Danube River in the Balkan region. BEN is a chronic tubulointerstitial disease with a slow progression to terminal renal failure and has strong association with upper tract urothelial carcinoma (UTUC). There are several hypotheses about the etiology of BEN, but only the toxic effect of aristolochic acid has been confirmed as a risk factor in the occurrence of the disease. Aberrantly expressed miRNAs have been shown to be associated with many types of cancers. A number of studies have investigated the expression of microRNAs in urothelial carcinoma, mainly on urothelial bladder cancer, and only a few have included patients with UTUC. Here we present the first study of microRNA profiling in UTUC tissues from patients with BEN (BEN-UTUC) and patients with UTUC from nonendemic Balkan regions (non-BEN-UTUC) in comparison to normal kidney tissues. We found 10 miRNAs that were differentially expressed in patients with BEN-UTUC and 15 miRNAs in patients with non-BEN-UTUC. miRNA signature determined in BEN-UTUC patients differs from the non-BEN-UTUC patients; only miR-205-5p was mutual in both groups. more...
- Published
- 2016
- Full Text
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8. HPV E6/E7 mRNA versus HPV DNA biomarker in cervical cancer screening of a group of Macedonian women.
- Author
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Duvlis S, Popovska-Jankovic K, Arsova ZS, Memeti S, Popeska Z, and Plaseska-Karanfilska D
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- Adult, Aged, Alphapapillomavirus genetics, Atypical Squamous Cells of the Cervix virology, Biomarkers, DNA, Viral analysis, Female, Greece, Humans, Middle Aged, Multiplex Polymerase Chain Reaction, Papillomavirus Infections virology, RNA, Messenger analysis, RNA, Messenger genetics, RNA, Viral genetics, Sensitivity and Specificity, Uterine Cervical Dysplasia diagnosis, Uterine Cervical Neoplasms virology, Young Adult, Early Detection of Cancer methods, Human Papillomavirus DNA Tests, Oncogene Proteins, Viral genetics, Papillomaviridae genetics, RNA, Viral analysis, Uterine Cervical Neoplasms diagnosis, Uterine Cervical Neoplasms prevention & control
- Abstract
High risk types of human papillomaviruses E6/E7 oncogenes and their association with tumor suppressor genes products are the key factors of cervical carcinogenesis. This study proposed them as specific markers for cervical dysplasia screening. The aim of the study is to compare the clinical and prognostic significance of HPV E6/E7 mRNA as an early biomarker versus HPV DNA detection and cytology in triage of woman for cervical cancer. The study group consists of 413 women: 258 NILM, 26 ASC-US, 81 LSIL, 41 HSIL, and 7 unsatisfactory cytology. HPV4AACE screening, real-time multiplex PCR and MY09/11 consensus PCR primers methods were used for the HPV DNA detection. The real-time multiplex nucleic acid sequence-based assay (NucliSENS EasyQ HPV assay) was used for HPV E6/E7 mRNA detection of the five most common high risk HPV types in cervical cancer (16, 18, 31, 33, and 45). The results show that HPV E6/E7 mRNA testing had a higher specificity 50% (95% CI 32-67) and positive predictive value (PPV) 62% (95% CI 46-76) for CIN2+ compared to HPV DNA testing that had specificity of 18% (95% CI 7-37) and PPV 52% (95% CI 39-76) respectively. The higher specificity and PPV of HPV E6/E7 mRNA testing are valuable in predicting insignificant HPV DNA infection among cases with borderline cytological finding. It can help in avoiding aggressive procedures (biopsies and over-referral of transient HPV infections) as well as lowering patient's anxiety and follow up period., (© 2015 Wiley Periodicals, Inc.) more...
- Published
- 2015
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9. Molecular characterization of cystinuria in south-eastern European countries.
- Author
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Popovska-Jankovic K, Tasic V, Bogdanovic R, Miljkovic P, Golubovic E, Soylu A, Saraga M, Pavicevic S, Baskin E, Akil I, Gregoric A, Lilova M, Topaloglu R, Sukarova Stefanovska E, and Plaseska-Karanfilska D more...
- Subjects
- Child, Child, Preschool, Cystinuria diagnosis, Europe, Female, Genotype, Humans, Male, Mutation, Amino Acid Transport Systems, Basic genetics, Amino Acid Transport Systems, Neutral genetics, Cystinuria genetics
- Abstract
Cystinuria is an autosomal recessive disorder caused by defective transport of cystine and dibasic amino acids in the proximal renal tubules and small intestine. So far, more than 128 mutations in SLC3A1 gene, and 93 in SLC7A9 gene have been described as a cause of cystinuria. We present a molecular characterization of the cystinuria in 47 unrelated south-east European families. The molecular methodology included direct sequencing, single strand conformational polymorphism, and restriction fragment length polymorphism. A total of 93 (94.9 %) out of 98 unrelated cystinuric chromosomes have been characterized. Mutations in SLC3A1 gene account for 64.3 % and in SLC7A9 gene for 30.6 % of the cystinuric chromosomes. Ten different mutations in SLC3A1 gene were found, and two of them were novel (C242R and L573X), while in SLC7A9 gene seven mutations were found, of which three were novel (G73R, V375I and c.1048_1051delACTC). The most common mutations in this study were T216M (24.5 %), M467T (16.3 %) and R365L (11.2 %) in SLC3A1 and G105R (21.4 %) in SLC7A9 gene. A population specificity of cystinuria mutations was observed; T216M mutation was the only mutation present among Gypsies, G105R was the most common mutation among Albanians and Macedonians, and R365L among Serbs. The results of this study allowed introduction of rapid, simple and cost-effective genetic diagnosis of cystinuria that enables an early preventive care of affected patients and a prenatal diagnosis in affected families. more...
- Published
- 2013
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10. Cystinuria AA (B): digenic inheritance with three mutations in two cystinuria genes.
- Author
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Gucev Z, Ristoska-Bojkovska N, Popovska-Jankovic K, Sukarova-Stefanovska E, Tasic V, Plaseska-Karanfilska D, and Efremov GD
- Subjects
- Child, Heterozygote, Humans, Kidney Calculi genetics, Male, Amino Acid Transport Systems, Basic genetics, Amino Acid Transport Systems, Neutral genetics, Cystinuria genetics, Mutation
- Published
- 2011
- Full Text
- View/download PDF
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