Author: "Popot, Jean-Luc" - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"Popot, Jean-Luc"' showing total 504 results

Start Over Author "Popot, Jean-Luc"

504 results on '"Popot, Jean-Luc"'

1. Co-delivery of amphipol-conjugated adjuvant with antigen, and adjuvant combinations, enhance immune protection elicited by a membrane protein-based vaccine against a mucosal challenge with Chlamydia

Author: Tifrea, Delia F, Pal, Sukumar, Le Bon, Christel, Giusti, Fabrice, Popot, Jean-Luc, Cocco, Melanie J, Zoonens, Manuela, and de la Maza, Luis M
Subjects: Biomedical and Clinical Sciences, Immunization, Vaccine Related, Infectious Diseases, Prevention, 3.4 Vaccines, Infection, Adjuvants, Immunologic, Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Bacterial Vaccines, Chlamydia Infections, Chlamydia muridarum, Female, Mice, Mice, Inbred BALB C, Chlamydia, Vaccine, Amphipols, A8-35, Adjuvant, EP-67, MOMP, Biological Sciences, Agricultural and Veterinary Sciences, Medical and Health Sciences, Virology, Biomedical and clinical sciences, Health sciences
Abstract: IntroductionChlamydial infections are spread worldwide and a vaccine is needed to control this pathogen. The goals of this study were to determine if the delivery of an adjuvant associated to the antigen, via a derivatized amphipol, and adjuvant combinations improve vaccine protection.MethodsA novel approach, trapping the Chlamydia muridarum (Cm) native MOMP (nMOMP) with amphipols (A8-35), bearing a covalently conjugated peptide (EP67), was used. Adjuvants incorporated were: EP67 either conjugated to A8-35, which was used to trap nMOMP (nMOMP/EP67-A8-35), or free as a control, added to nMOMP/A8-35 complexes (nMOMP/A8-35+EP67); Montanide ISA 720 to enhance humoral responses, and CpG-1826 to elicit robust cell-mediated immunity (CMI). BALB/c mice were immunized by mucosal and systemic routes. Intranasal immunization with live Cm was used as positive control and three negative controls were included. Mice were challenged intranasally with Cm and changes in body weight, lungs weight and number of Cm-inclusion forming units (IFU) recovered from the lungs were evaluated to establish protection. To assess local responses levels of IFN- γ and Cm-specific IgA were determined in lungs' supernatants.ResultsStructural assays demonstrated that nMOMP secondary structure and thermal stability were maintained when A8-35 was covalently modified. Mice vaccinated with nMOMP/EP67-A8-35 were better protected than animals immunized with nMOMP/A8-35+EP67. Addition of Montanide enhanced Th2 responses and improved protection. Including CpG-1826 further broadened, intensified and switched to Th1-biased immune responses. With delivery of nMOMP and the three adjuvants, as determined by changes in body weight, lungs weight and number of IFU recovered from lungs, protection at 10 days post-challenge was equivalent to that induced by immunization with live Cm.ConclusionsCovalent association of EP67 to A8-35, used to keep nMOMP water-soluble, improves protection over that conferred by free EP67. Adjuvant combinations including EP67+Montanide+CpG-1826, by broadening and intensifying cellular and humoral immune responses, further enhanced protection.
Published: 2018

2. Optical Spectroscopy of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

3. Solution Studies of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

4. Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

5. Amphipol-Assisted Cell-Free Expression of Membrane Proteins

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

6. Amphipol-Assisted Folding of Membrane Proteins

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

7. Alternatives to Detergents for Handling Membrane Proteins in Aqueous Solutions

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

8. Formation and Properties of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

9. Membrane Proteins and Their Natural Environment

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

10. Extracting Membrane Proteins from Their Native Environment

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

11. Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

12. Biomedical Applications

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

13. The Use of Amphipols in Mass Spectrometry

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

14. The Use of Amphipols for Electron Microscopy

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

15. Amphipols and Membrane Protein Crystallization

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

16. Nuclear Magnetic Resonance Studies of Amphipol-Trapped Membrane Proteins

Author: Popot, Jean-Luc, Aizawa, Masuo, Series Editor, Austin, Robert H., Series Editor, Gerstman, Bernard S., Editor-in-Chief, Barber, James, Series Editor, Berg, Howard C., Series Editor, Callender, Robert, Series Editor, Feher, George, Series Editor, Frauenfelder, Hans, Series Editor, Giaever, Ivar, Series Editor, Joliot, Pierre, Series Editor, Keszthelyi, Lajos, Series Editor, King, Paul W., Series Editor, Lazzi, Gianluca, Series Editor, Lewis, Aaron, Series Editor, Lindsay, Stuart M., Series Editor, Liu, Xiang Yang, Series Editor, Mauzerall, David, Series Editor, Mielczarek, Eugenie V., Series Editor, Niemz, Markolf, Series Editor, Parsegian, V. Adrian, Series Editor, Powers, Linda S., Series Editor, Prohofsky, Earl W., Series Editor, Rostovtseva, Tatiana K., Series Editor, Rubin, Andrew, Series Editor, Seibert, Michael, Series Editor, Tao, Nongjian, Series Editor, Thomas, David, Series Editor, and Popot, Jean-Luc
Published: 2018
Full Text: View/download PDF

17. BAmSA: Visualising transmembrane regions in protein complexes using biotinylated amphipols and electron microscopy

Author: Perry, Thomas Noe, Souabni, Hager, Rapisarda, Chiara, Fronzes, Rémi, Giusti, Fabrice, Popot, Jean-Luc, Zoonens, Manuela, and Gubellini, Francesca
Published: 2019
Full Text: View/download PDF

18. Long-Term Stability of a Vaccine Formulated with the Amphipol-Trapped Major Outer Membrane Protein from Chlamydia trachomatis

Author: Eric Feinstein, H, Tifrea, Delia, Sun, Guifeng, Popot, Jean-Luc, de la Maza, Luis M, and Cocco, Melanie J
Subjects: Biochemistry and Cell Biology, Biological Sciences, Infectious Diseases, Immunization, Biotechnology, Sexually Transmitted Infections, Prevention, Vaccine Related, 3.4 Vaccines, Infection, Bacterial Outer Membrane Proteins, Bacterial Vaccines, Chemistry, Pharmaceutical, Chlamydia trachomatis, Drug Carriers, Drug Evaluation, Preclinical, Drug Stability, Hydrophobic and Hydrophilic Interactions, Protein Conformation, Protein Denaturation, Solubility, Surface-Active Agents, Amphipols, Vaccine, Stability, MOMP, NMR, Microbiology, Medical Microbiology, Physiology, Biochemistry and cell biology
Abstract: Chlamydia trachomatis is a major bacterial pathogen throughout the world. Although antibiotic therapy can be implemented in the case of early detection, a majority of the infections are asymptomatic, requiring the development of preventive measures. Efforts have focused on the production of a vaccine using the C. trachomatis major outer membrane protein (MOMP). MOMP is purified in its native (n) trimeric form using the zwitterionic detergent Z3-14, but its stability in detergent solutions is limited. Amphipols (APols) are synthetic polymers that can stabilize membrane proteins (MPs) in detergent-free aqueous solutions. Preservation of protein structure and optimization of exposure of the most effective antigenic regions can avoid vaccination with misfolded, poorly protective protein. Previously, we showed that APols maintain nMOMP secondary structure and that nMOMP/APol vaccine formulations elicit better protection than formulations using either recombinant or nMOMP solubilized in Z3-14. To achieve a greater understanding of the structural behavior and stability of nMOMP in APols, we have used several spectroscopic techniques to characterize its secondary structure (circular dichroism), tertiary and quaternary structures (immunochemistry and gel electrophoresis) and aggregation state (light scattering) as a function of temperature and time. We have also recorded NMR spectra of (15)N-labeled nMOMP and find that the exposed loops are detectable in APols but not in detergent. Our analyses show that APols protect nMOMP much better than Z3-14 against denaturation due to continuous heating, repeated freeze/thaw cycles, or extended storage at room temperature. These results indicate that APols can help improve MP-based vaccine formulations.
Published: 2014

19. Increased Immunoaccessibility of MOMP Epitopes in a Vaccine Formulated with Amphipols May Account for the Very Robust Protection Elicited against a Vaginal Challenge with Chlamydia muridarum

Author: Tifrea, Delia F, Pal, Sukumar, Popot, Jean-Luc, Cocco, Melanie J, and de la Maza, Luis M
Subjects: Medical Microbiology, Biomedical and Clinical Sciences, Immunology, Vaccine Related, Infectious Diseases, Immunization, Sexually Transmitted Infections, Prevention, 3.4 Vaccines, Infection, Good Health and Well Being, Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Bacterial Vaccines, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Chlamydia Infections, Chlamydia muridarum, Female, Mice, Mice, Inbred BALB C, Pregnancy, Propylamines, Biochemistry and cell biology
Abstract: There is a need to implement a vaccine to protect against Chlamydia trachomatis infections. To test a new vaccine, mice were immunized with the Chlamydia muridarum native major outer membrane protein (nMOMP) solubilized with either amphipol A8-35 or the detergent Z3-14. OVA was used as a negative control, and mice were inoculated intranasally with C. muridarum as positive controls. Animals vaccinated with nMOMP mounted strong Chlamydia-specific humoral and cell-mediated immune responses. Mice vaccinated with nMOMP/A8-35 had a higher ratio of Abs to denatured elementary bodies (EB) over live EB, recognized more synthetic MOMP peptides and had higher neutralizing titers than sera from mice immunized with nMOMP/Z3-14. T cell lymphoproliferative responses and levels of IFN-γ were also higher in mice vaccinated with nMOMP/A8-35 than with nMOMP/Z3-14. Following immunization, animals were challenged intravaginally with C. muridarum. On the basis of the number of mice with positive vaginal cultures, length of vaginal shedding, total number of positive vaginal cultures, and number of Chlamydia inclusion forming units recovered, nMOMP/A8-35 elicited a more robust protection than nMOMP/Z3-14. By depleting T cells with Abs, we determined that CD4(+) and not CD8(+) T cells mediated the protection elicited by nMOMP/A8-35. Mice were subsequently mated, and based on the number of pregnant mice and number of embryos, animals that were vaccinated with nMOMP/A8-35 or nMOMP/Z3-14 had fertility rates equivalent to the positive control group immunized with live EB and the fertility controls. In conclusion, increased accessibility of epitopes in the nMOMP/A8-35 preparation may account for the very robust protection against infection and disease elicited by this vaccine.
Published: 2014

20. La structure des protéines

Author: Yon-Kahn, Jeannine, primary, Hervé, Guy, additional, and Popot, Jean-Luc, additional
Published: 2020
Full Text: View/download PDF

21. Folding and stabilizing membrane proteins in amphipol A8-35

Author: Le Bon, Christel, Marconnet, Anaïs, Masscheleyn, Sandrine, Popot, Jean-Luc, and Zoonens, Manuela
Published: 2018
Full Text: View/download PDF

22. NMR Study of a Membrane Protein in Detergent-Free Aqueous Solution

Author: Zoonens, Manuela, Catoire, Laurent J., Giusti, Fabrice, Popot, Jean-Luc, and Engelman, Donald M.
Published: 2005

23. Amphipols: Polymers that Keep Membrane Proteins Soluble in Aqueous Solutions

Author: Tribet, Christophe, Audebert, Roland, and Popot, Jean-Luc
Published: 1996

24. Amphipols: A General Introduction and Some Protocols

Author: Zoonens, Manuela, Zito, Francesca, Martinez, Karen L., Popot, Jean-Luc, and Mus-Veteau, Isabelle, editor
Published: 2014
Full Text: View/download PDF

25. Folding and stability of integral membrane proteins in amphipols

Author: Kleinschmidt, Jörg H. and Popot, Jean-Luc
Published: 2014
Full Text: View/download PDF

26. Folding membrane proteins in vitro: A table and some comments

Author: Popot, Jean-Luc
Published: 2014
Full Text: View/download PDF

27. Extracting Membrane Proteins from Their Native Environment

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

28. Amphipol-Assisted Cell-Free Expression of Membrane Proteins

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

29. Biomedical Applications

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

30. The Use of Amphipols for Electron Microscopy

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

31. Amphipols and Membrane Protein Crystallization

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

32. Alternatives to Detergents for Handling Membrane Proteins in Aqueous Solutions

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

33. Amphipol-Mediated Immobilization of Membrane Proteins and Its Applications

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

34. Nuclear Magnetic Resonance Studies of Amphipol-Trapped Membrane Proteins

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

35. Optical Spectroscopy of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

36. Formation and Properties of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

37. Membrane Proteins and Their Natural Environment

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

38. Amphipol-Assisted Folding of Membrane Proteins

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

39. Chemical Structure, Synthesis, and Physical-Chemical Properties of Amphipols

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

40. Solution Studies of Membrane Protein/Amphipol Complexes

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

41. The Use of Amphipols in Mass Spectrometry

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

42. Membrane Proteins in Aqueous Solutions

Author: Popot, Jean-Luc, primary
Published: 2018
Full Text: View/download PDF

43. Folding of diphtheria toxin T-domain in the presence of amphipols and fluorinated surfactants: Toward thermodynamic measurements of membrane protein folding

Author: Kyrychenko, Alexander, Rodnin, Mykola V., Vargas-Uribe, Mauricio, Sharma, Shivaji K., Durand, Grégory, Pucci, Bernard, Popot, Jean-Luc, and Ladokhin, Alexey S.
Published: 2012
Full Text: View/download PDF

44. The Use of Amphipols as Universal Molecular Adapters to Immobilize Membrane Proteins onto Solid Supports

Author: Charvolin, Delphine, Perez, Jean-Baptiste, Rouvière, Florent, Giusti, Fabrice, Bazzacco, Paola, Abdine, Alaa, Rappaport, Fabrice, Martinez, Karen L., and Popot, Jean-Luc
Published: 2009
Full Text: View/download PDF

45. On peut marcher sur l'eau, les guêpes le font à Suzon: Ce phénomène étonnant en dit long sur l'extraordinaire structure de l'eau, sans laquelle la Vie, telle que nous la connaissons, n'existerait pas.

Author: POPOT, Jean-Luc
Subjects: MOLECULAR structure of water, SURFACE tension, HYDROGEN bonding, HYDROPHOBIC interactions, ORGANISMS
Abstract: The article focuses on the extraordinary properties of water, specifically its surface tension, which allows insects like wasps and water striders to walk on its surface. It explains how the molecular structure of water, with its hydrogen bonds, leads to these behaviors and plays a crucial role in the organization of living organisms, emphasizing the significance of the hydrophobic effect in biological processes.
Published: 2023

46. Amphipols stabilize the Chlamydia major outer membrane protein and enhance its protective ability as a vaccine

Author: Tifrea, Delia F., Sun, Guifeng, Pal, Sukumar, Zardeneta, Gustavo, Cocco, Melanie J., Popot, Jean-Luc, and de la Maza, Luis M.
Published: 2011
Full Text: View/download PDF