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In social marketing definition, green marketing is the improvement and marketing of products layout to as far as possible minimize adverse effect level on the physical environment. As for environmental marketing definition, green marketing is defined for organizations endeavour to produce, promote, package and reclaim products in a manner that susceptible or responsive to environmental concerns. In this context we define green marketing performing marketing activities which include product improvement, pricing, promote and placing so as to minimize harm to the environment regardless of sector. Related to this approach it will be made a survey in order to analyse the attitude of young generation toward green marketing. It will be created a questionnaire that could offer a perspective about the behaviour of young people concern the environmental issues and future challenges.

Research background: We develop a conceptual framework based on a systematic and comprehensive literature review on smartphones as consumption tools globally. Purpose of the article: Building our argument by drawing on data collected from Comscore, Econsultancy, and Statista, we performed analyses and made estimates regarding the adoption of Internet-enabled devices for shopping purposes: selected retail and shopping activities of EU smartphone users, most popular mobile shopping apps in the U.S. (by reach), adults in the EU who never make purchases on mobile devices (by gender), and consumers who use a mobile device for product research while shopping in-store worldwide (by country). Methods: The data for this research were gathered via an online survey questionnaire and were analyzed through structural equation modeling on a sample of 4,200 respondents. Findings & Value added: Smartphones have permeated the online community swiftly. The difficult task retailers confront is in obtaining consumers’ mutual support in supplying the information required to provide a customized shopping experience. Mobile payment privacy is associated with apprehensions that users may have concerning what will become of the data supplied when they adopt mobile payment. A satisfactory array of facilitating circumstances brings about greater purpose to adopt mobile shopping applications, especially when diverse viable functions such as convenience and swift purchase are provided. Intention to raise subsequent benefits from mobile buying should harmonize the lasting value predisposition of the mobile purchasers. The essential issue encountered by the service suppliers is how to turn the developing mobile payment operation into financial earnings.

The states from European Union must take advantage from renewable energy sources in order to revive the economy. Climate change creates new jobs and could reduce energy imports which would stimulate the economy of those states. The European Union should support research in the field, apply more efficient policies in energy, and create economies of scale to get an integrated European energy market in which the main actors can reduce the cost of production of renewable energy. In addition, it is possible to use the comparative advantages of the Member States and not only, like Greece, through solar energy, Southern Mediterranean, through distribution networks interconnections with EU, Russia and Ukraine, through biomass and the North Sea, through wind energy. This paper refers to the evolution and trends of the renewable energy sources and presents some scenarios of it.

In the context of intensification of the economic uncertainty and competition, companies need to know and build the essential elements that ensure success as better as they can - it is about management, technology and smart use of human resources. Enhancing these essential elements is achieved by the Human Resources Department within the organization, and hence they constitute a crucial aspect of the recovery potential in an organization.

Gross inland energy consumption represents the quantity of energy necessary to satisfy inland consumption of the geographical entity under consideration. It describes the total energy needs of a country. According to some Eurostat reports from 2013 and 2014, over the last two decades, gross inland energy consumption in the EU28, as can be noted, has changed pretty much: in 1990 cumulated 1670 million tonnes of oil equivalent (Mtoe), rose to a peak of 1830 Mtoe in 2006 and then decreased to 1680 Mtoe in 2012. These contradictory values required a comprehensive analysis and therefore, present paper describes the main characteristics related to the grouping EU Member States according to gross inland energy consumption in order to discover some common patterns among EU member states.

The aim of this paper is that of identifying the characteristics of the evolution of the primary production of natural gas in the EU-28 as well as in other non-EU countries from 2003 to 2014.

Heavy metals in contaminated soils have benefited from a considerable attention due to the possible risks for the human body. The current study has investigated the accumulation and transfer coefficient for three heavy metals (Cu, Pb, Zn) found in the contaminated soil with three concentrations (c1=1.5%, c2=3.0%, c3=4.5%, c4=6.0%), obtained by mixing the three metals, in the tomato fruit. The highest accumulation in the tomato fruits was recorded for zinc, then copper and the smallest for lead, for all four concentrations used. The transfer coefficient decreases as the concentration of heavy metals increases, so that for high heavy metals concentrations, the values of the transfer coefficient are very low, and for small heavy metals concentrations in the soil, the values for the transfer coefficient are higher. The assessment of accumulation and transfer of heavy metals in the fruits of tomatoes grown in the contaminated soil has concluded that all concentrations of the copper, lead and zinc mix have shown a low risk for human consumption.

Worldwide the processes of erosion, transport and sedimentation have a significant social, economic and environmental impact. In the past decade the most important external factor that has significantly increased the erosion and sediment process is the human factor throughout its daily activities. The changes of land cover and use, the intensive deforestation, urbanization and infrastructure development, sand extraction from river beds alter the natural watercourse and thus leading to erosion, sediment transportation and finally to the silting of rivers and reservoirs. The process of erosion and sedimentation occurs, among other areas, in the hydro-technical constructions with significant effects. In the next 30 years it’s expected to lose more than 50% of the storage capacity of the reservoirs in the world due to the silting phenomenon. The control and monitor of the sediment dynamics represents a critical component in the sustainable management of water resources. Also the understanding of the involved phenomena is essential in order to develop solution for reducing the sediment and erosion that occur and adopt measures to extend the life of the reservoirs. This paper taps the solution of mathematical modelling using dedicated software in order to establish the morphological changes that occur in Ogrezeni-Crivina reservoir, located on the river Arges.

The project of preventing the natural hazards and protection against floods of city Babadag has been designed to protect river banks of Tabana brook and its tributaries inside the area of city Babadag, against floods and silting phenomenon. The project contributes to the protection of infrastructure and social economic objectives, being in danger to deteriorate or even to be destroyed if this project will not be implemented. Aside protection of infrastructure and economic objectives, this study has another extremely important purpose for the community protection and improvement of the environmental quality and living standards from the area.

The whole process of getting a job refers to a set of measures, from the identification of the opportunity to offer a new job to the consolidation of the relationship with the new employee starting the first day after accepting the job. Once the opportuni

Background/objectives: This study aimed to investigate the lifestyle and the behavioral factors that influence the nutritional status of adolescents from Transylvania, Romania., Methods: The Global School-Based Student Health Survey (GSHS) was used to collect data from 900 adolescents between 11 and 18 years old from the Transylvania region, Romania. This study assessed nutritional status by calculating BMI indicators adjusted to Z-Score, cut-off points according to the World Health Organization (WHO), using self-reported weight and height; perceived health status; food vulnerability; physical activity; addictive behaviors (cigarette, alcohol and drug consumption); number of hours spent in front of the computer/phone; hand and oral hygiene; sitting time/day; and sleep. Multivariate logistic regression was used to establish the lifestyle factors that influenced nutritional status., Results: The results showed that 8.7% ( n = 78) of girls and 15.2% ( n = 137) boys were overweight and obese. In total, 75% of the respondents were engaged in sedentary behaviors, and 65.8% ( n = 592) had more than 2 h/day of screen exposure, considering that 98.7% of the study population had a mobile phone. The Romanian adolescents had poor dietary behaviors: over 80% of them did not meet the recommended amount of vegetable and fruit intake per day. Increased BMI was associated with higher-strength physical exercise and with being a boy., Conclusions: While some positive trends are evident, such as good oral and hand hygiene and low prevalence of smoking and drug use, significant challenges remain in areas like nutrition, physical activity, alcohol consumption and screen time.

Campylobacter species, predominantly Campylobacter jejuni, remains a significant zoonotic pathogen worldwide, with the poultry sector being the primary vector for human transmission. In recent years. there has been a notable rise in the incidence of human campylobacteriosis, necessitating a deeper understanding of the pathogen's survival mechanisms and transmission dynamics. Biofilm presence significantly contributes to C. jejuni persistence in poultry and subsequent food product contamination, and this review describes the intricate processes involved in biofilm formation. The ability of Campylobacter to form biofilms on various surfaces, including stainless steel, plastic, and glass, is a critical survival strategy. Campylobacter biofilms, with their remarkable resilience, protect the pathogen from environmental stresses such as desiccation, pH extremes, biocides and sanitizing agents. This review explores the molecular and genetic mechanisms of C. jejuni biofilm formation, highlighting regulatory genes involved in motility, chemotaxis, and stress responses. Flagellar proteins, particularly flaA, flaB, flaG, and adhesins like cadF and flpA, are identified as the main molecular components in biofilm development. The role of mixed-species biofilms, where C. jejuni integrates into existing biofilms of other bacteria to enhance pathogen resilience, is also discussed. This review also considers alternative interventions to control C. jejuni in poultry production, in the context of increasing antibiotic resistance. It explores the effectiveness of prebiotics, probiotics, synbiotics, bacteriocins, bacteriophages, vaccines, and organic acids, with a focus on their mechanisms of action in reducing bacterial colonization and biofilm formation. Studies show that mixtures of organic acids and compounds like Carvacrol and Eugenol significantly downregulate genes linked with motility and adhesion, thereby disrupting biofilm integrity. It discusses the impact of environmental factors, such as temperature and oxygen levels on biofilm formation, providing insights into how industrial conditions can be manipulated to reduce contamination. This paper stresses the need for a multifaceted approach to control Campylobacter in poultry, integrating molecular and genetic insights with practical interventions. By advancing our understanding of biofilm dynamics and gene regulation, we aim to inform the development of more effective strategies to enhance food safety and protect public health., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

This study compares three Romanian Hyssopus officinalis species- H. officinalis f. ruber (HOR), H. officinalis f. albus (HOA), and H. officinalis f. cyaneus (HOC)-evaluating their chemical composition and biological activities, specifically protein denaturation, haemolysis inhibition, and antibacterial effects. Chemical profiles were determined using Gas Chromatography-Mass Spectrometry (GC-MS). The species were cultivated at two distinct locations: the Didactic and Experimental Station DESUSVT and the Agricultural Research and Development Station Lovrin (ARDSL). This study investigates the correlation between chemical composition, biological activities, and local climate data at each site. The results show significant variations in chemical profiles, with species and cultivation location influencing the biological activities. H. officinalis f. albus (HOA) exhibited the strongest antimicrobial activity, particularly against Gram-positive bacteria. The molecular docking analysis highlighted key compounds, such as cyclohexene,4-isopropenyl-1-methoxymethoxymethyl and elemol, with binding solid affinities to microbial and inflammatory proteins. This study provides valuable insights into the chemical and biological properties of Hyssopus officinalis , emphasising its potential in combating microbial infections, protein denaturation, and haemolysis inhibition.

This study investigated the phytochemical characteristics, antibacterial activity, and synergistic potential of essential oils derived from Romanian lavender. Gas Chromatography-Mass Spectrometry (GC/MS) analysis revealed that linalool is the main compound in all lavender essential oils, with concentrations ranging from 29.410% to 35.769%. Linalyl acetate was found in similar concentrations to linalool. Other significant compounds included 1,8-cineole (8.50%), lavandulyl acetate (5.38%), trans-β-ocimene (6.90%), and camphor (7.7%). A 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) test was used to assess antioxidant capacity, with substantial free-radical-scavenging activity shown in the IC 50 values determined. The antibacterial efficacy of the oils was higher against Gram-positive bacteria than Gram-negative bacteria, with variations in minimum inhibitory concentrations (MICs), the extent of inhibition, and evolution patterns. The study also explored the oils' ability to enhance the efficacy of ampicillin, revealing synergistic interactions expressed as fractional inhibitory concentration indices. In silico protein-ligand docking studies used twenty-one compounds identified by GC-MS with bacterial protein targets, showing notable binding interactions with SasG (-6.3 kcal/mol to -4.6 kcal/mol) and KAS III (-6.2 kcal/mol to -4.9 kcal/mol). Overall, the results indicate that Romanian lavender essential oils possess potent antioxidant and antibacterial properties, and their synergistic interaction with ampicillin has potential for enhancing antibiotic therapies.

Social media platforms like Instagram (Meta Platforms, Inc., Menlo Park, California, United States) and Snapchat   (Snap Inc., California, United States) significantly influence motivations for aesthetic surgery by promoting idealized and digitally enhanced images. Understanding their impact on body image dissatisfaction and acceptance of cosmetic procedures is crucial. A systematic review following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines explored the link between social media, body image dissatisfaction, and cosmetic surgery. The review included 25 studies with 13,731 participants. Specific findings revealed that 70% of young adult women and 60% of young adult men report dissatisfaction with their bodies, leading to increased surgical considerations. The search process utilized databases such as PubMed, ScienceDirect, and Google Scholar, employing keywords like "cosmetic surgery," "social media," and "body image dissatisfaction" for articles published between January 2013 and December 2023. Both men and women show increased dissatisfaction with body parts, leading to surgical considerations. Social media's emphasis on visual aesthetics fosters body dissatisfaction and social appearance anxiety, especially through selfies. Cultural norms and celebrity influence further shape beauty perceptions. While social media promotes cosmetic surgery acceptance, ethical concerns about misleading advertisements, unrealistic beauty standards, and patient privacy persist. This underscores the need for strategies to promote healthy body image and informed choices in the digital age., Competing Interests: Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: This research was funded by UMF Cluj-Napoca, grant number 647/39/11 January 2024. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Mironica et al.)

The editorial introduces the JBO special issue honoring Prof. Gabriel Popescu, Pioneer in Biomedical Optics., (© 2024 The Authors.)

Background and Objectives : This study aims to assess the health status and factors influencing healthy choices among children and young people in Romania, as well as the efficacy of related health education programs. Through understanding these dynamics, the study seeks to provide insights that can shape targeted interventions, policies, and educational strategies to improve this demographic's overall health and well-being. Materials and Methods : For this study, we performed a literature review of original published papers on the health status, healthy habits, health education, predisposition to making healthy choices in the future, and accessibility to the paediatric health system of Romanian children and young people, as well as the effects of different types of educational interventions on this demographic in Romania. Results : The prevalence of dental caries is high in Romania. In terms of eating habits and nutritional status, a worrying proportion of children are overweight or obese, which can lead to a variety of future physical and psychological problems. In terms of physical activity, few adolescents demonstrate regular fitness practices. Romania presents an increase in alcohol and tobacco consumption among adolescents. The mental health of students has become a pressing public health concern, exacerbated by the COVID-19 pandemic. The use of social networks is linked to mental health issues among young people. Romania still has one of the highest rates of sexually transmitted diseases and faces a high incidence of cervical cancer, with a mortality rate three times higher than the EU average. High rates of teenage pregnancies are linked to limited information about sexuality and a lack of access to family planning at a young age. There are large discrepancies in the accessibility of medical services between urban and rural areas. Conclusions : Romania faces significant obstacles to providing high-quality healthcare to children and young people. Improving nutrition, immunisation rates, and access to medical services represent essential areas for enhancing the health of children and young people in Romania.

Background: This article investigates the determinants of the intention to migrate of nursing students at a major medical university in Romania and relates them to major international developments, specifically the Brexit referendum and the COVID-19 pandemic., Methods: An online survey about the intention to migrate was made available to nursing students at the University of Medicine and Pharmacy "Iuliu Hațieganu", Cluj-Napoca, Romania, in 2016 (before Brexit) and again in 2016 (after Brexit), 2017, 2018, and 2021 and 2022 (during the pandemic). A total of 549 students responded (response rate: 84.6%)., Results: Before the Brexit referendum, 62.6% of the respondents had a plan to seek employment abroad, whereas after the Brexit referendum, only 34.7% indicated that they had such a plan after graduation. Before the pandemic, 43.6% of the students expressed an intention to work abroad, while during the pandemic, only 19.8% had such plans., Conclusions: This study documented the effect of significant international developments-such as the Brexit referendum and the COVID-19 pandemic-on decreasing the intention to migrate. As expected, the change in preference for the UK as a destination country changed dramatically. Additionally, the study provides both theoretical and empirical insights into the types of and the consistency of preparation for migration of nursing students.

Psoriasis is a chronic skin disease that affects a significant number of patients and can severely impair quality of life. Although the diagnosis is normally clinical, paraclinical determination can occasionally be useful either in differential diagnosis or in evaluating the inflammatory response to treatment. MicroRNAs (miRNAs) are small non-coding parts of the RNA family that regulate gene expression and may have an important role as biomarkers in evaluating treatment response. The dysregulation of miRNAs has been well studied in other diseases, especially in oncology, but their role in chronic skin conditions such as psoriasis is still not fully understood. This study aims to evaluate the levels of three miRNAs (miR-155, miR-210, and miR-205) in patients with psoriasis, treated either systemically or topically, compared to a control group, and to assess the possible relationship between miRNA levels and systemic therapy. Our findings show a constant dysregulation of miR-205 in patients with psoriasis, with significantly higher levels compared to the control group, which can be explained as conferring a protective effect to treated patients. Further studies are needed in order to fully understand the role of miRNAs in the physiopathology of psoriasis and even, potentially, to provide more targeted genetic therapies in the future., Competing Interests: The authors declare no conflict of interest., (© 2024 by the authors.)

Background: Atopic dermatitis (AD) at a young age often precedes the development of food allergies. Although AD affects millions of infants worldwide, prenatal and postnatal risk factors, and their association with the development of food allergies later on, are not fully elucidated. This study seeks to investigate AD epidemiology in infancy and its risk factors, examining early-life factors (both prenatal and postnatal) that could contribute to the later development of food allergies., Methods: Between January 2019 and December 2019, 501 infants were included in this prospective cohort study. Longitudinal data collection was performed through maternal interviews, the first one conducted within three days after the delivery and the second within 24 to 36 months after the delivery, encompassing variables such as demographics, family history of atopy, maternal smoking, antibiotic use during pregnancy, the mode of delivery, breastfeeding history, food practices, and greenness exposure within 3 days from delivery, while they were still in the hospital., Results: Maternal smoking during pregnancy ( p = 0.001) and an older sibling atopy history ( p = 0.03) was significantly linked to AD incidence. Cesarean section delivery ( p = 0.04) was associated with a higher risk of food allergies in infants with AD. Having a garden at home correlated with a higher likelihood of AD ( p = 0.01), and food elimination without medical guidance ( p = 0.02) due to AD correlated with an elevated risk of food allergies., Conclusions: Encouraging timely allergenic food introduction while promoting dietary diversity, rich in plant-based foods, maternal smoking cessation, and professional dietary guidance may help minimize AD and food allergy risk. Future studies should address the role of greenness in the development of AD and food allergies.

Background and Objectives : The ongoing COVID-19 pandemic has posed an array of new challenges for medical students worldwide. Amidst academic rigors, students are confronted with unique stressors, potentially affecting their mental health and substance use. This study aimed to investigate the multifaceted effects of depression, alcohol use, and stress on medical students and discern how these factors have been amplified by the pandemic's circumstances, and to identify predictors of mental distress during the COVID-19 pandemic period. Materials and Methods : Two online anonymous and cross-sectional surveys were conducted at the University of Medicine and Pharmacy "Iuliu Hatieganu" in Romania among medical students in 2018 and in 2022. Data were gathered via online questionnaires distributed through closed student groups on social media platforms, with a total of 1061 participants, to investigate stress, depression, alcohol and drug use, and the impact of the pandemic and online education on mental health, maintaining anonymity and ethical approval. The Medical Student Stress Questionnaire (MSSQ) was employed to measure different aspects of stress, the Beck Depression Inventory (BDI) provided insights into the participants' depressive symptoms, and for the assessment of alcohol consumption habits, the Alcohol Use Disorders Identification Test (AUDIT) was utilized. Results : Our findings showed a significant decrease in mean depression scores (13.81 vs. 11.56, with p < 0.001) from 2018 to 2022. In 2018, students scored significantly higher in the overall stress-related domains. Additionally, being female, facing financial constraints, and being in pre-clinical years emerged as predictors of heightened academic-related stress and depressive symptoms. Students who had experienced the loss of a family member due to COVID-19 exhibited a statistically significant rise in their average BDI score and current anxiety levels. Conclusions : The pandemic, despite introducing new stressors, may have indirectly fostered an increased focus on students' mental health, leading to more refined support mechanisms. Specialized interventions, taking into account gender and financial problems, are needed to address the multifaceted challenges faced by medical students. Our study highlights the ongoing need to nurture both the academic and emotional strength of future medical practitioners.

Background: Psoriasis is one of the most frequent chronic inflammatory skin diseases and has a negative impact on the interpersonal relationship and psychosocial well-being. The aims of this study were to examine the effects of intensity of pruritus on quality of life and depression, to investigate the relationship between anger, self-esteem, and depression, and to compare patients with early and late onset of psoriasis. As our study was carried out during the COVID-19 pandemic, we aimed also to investigate the safety concerns and anxiety related to COVID-19 in psoriasis patients., Methods: This cross-sectional study included 137 patients diagnosed with plaque psoriasis. The patients were classified as early-onset (age < 30 years) and late-onset psoriasis (age ≥ 30 years). Duration of disease, pruritus scores, and socio-demographic characteristics were recorded. Measures included the State-Trait Anger Expression Inventory (STAXI), Rosenberg Self-Esteem Scale, Beck Depression Inventory (BDI-II), Psoriasis Disability Index (PDI), and Fear and anxiety in relationship with COVID-19 Scale were used for determining anger, anger expression style, self-esteem, depression, anxiety, and quality of life., Results: The psoriasis patients had a lower score for self-esteem than the normative data from the Romanian general population. The average scores for state anger and trait anger are similar to the normative data from the Romanian general population, but the scores for anger-in and anger-out are higher. Patients with early onset had higher depression scores and lower quality of life. Self-esteem correlates negatively with depression, anger, severity of disability due to psoriasis, number of affected areas, and duration of disease. Lower level of self-esteem led to increased anger., Conclusions: Reduced self-esteem, increased anger levels, and depression are present in psoriasis patients. The effective treatment of psoriasis must, therefore, consist of a multidisciplinary approach, in which the personalized treatment of the skin condition is as important as the adjuvant therapies that reduce the patients' stress level.

The objective of this work was to investigate, for the first time, the antioxidant effect of a mixture of natural antimicrobials in an Enterocytozoon hepatopenaei (EHP) shrimp-gut model of infection and the biological mechanisms involved in their way of action. The study approach included investigations, firstly, in vitro, on shrimp-gut primary (SGP) epithelial cells and in vivo by using EHP-challenged shrimp. Our results show that exposure of EHP spores to 0.1%, 0.5%, 1%, and 2% AuraAqua (Aq) significantly reduced spore activity at all concentrations but was more pronounced after exposure to 0.5% Aq. The Aq was able to reduce EHP infection of SGP cells regardless of cells being pretreated or cocultured during infection with Aq. The survivability of SGP cells infected with EHP spores was significantly increased in both scenarios; however, a more noticeable effect was observed when the infected cells were pre-exposed to Aq. Our data show that infection of SGP cells by EHP activates the host NADPH oxidases and the release of H 2 O 2 produced. When Aq was used during infection, a significant reduction in H 2 O 2 was observed concomitant with a significant increase in the levels of CAT and SOD enzymes. Moreover, in the presence of 0.5% Aq, the overproduction of CAT and SOD was correlated with the inactivation of the NF-κB pathway, which, otherwise, as we show, is activated upon EHP infection of SGP cells. In a challenge test, Aq was able to significantly reduce mortality in EHP-infected shrimp and increase the levels of CAT and SOD in the gut tissue. Conclusively, these results show, for the first time, that a mixture of natural antimicrobials (Aq) can reduce the EHP-spore activity, improve the survival rates of primary gut-shrimp epithelial cells and reduce the oxidative damage caused by EHP infection. Moreover, we show that Aq was able to stop the H 2 O 2 activation of the NF-κB pathway of Crustins, Penaeidins, and the lysozyme, and the CAT and SOD activity both in vitro and in a shrimp challenge test.

The minichromosome maintenance (MCM) complex acts as a DNA helicase during DNA replication, and thereby regulates cell cycle progression and proliferation. In addition, MCM-complex components localize to centrosomes and play an independent role in ciliogenesis. Pathogenic variants in genes coding for MCM components and other DNA replication factors have been linked to growth and developmental disorders as Meier-Gorlin syndrome and Seckel syndrome. Trio exome/genome sequencing identified the same de novo MCM6 missense variant p.(Cys158Tyr) in two unrelated individuals that presented with overlapping phenotypes consisting of intra-uterine growth retardation, short stature, congenital microcephaly, endocrine features, developmental delay and urogenital anomalies. The identified variant affects a zinc binding cysteine in the MCM6 zinc finger signature. This domain, and specifically cysteine residues, are essential for MCM-complex dimerization and the induction of helicase activity, suggesting a deleterious effect of this variant on DNA replication. Fibroblasts derived from the two affected individuals showed defects both in ciliogenesis and cell proliferation. We additionally traced three unrelated individuals with de novo MCM6 variants in the oligonucleotide binding (OB)-fold domain, presenting with variable (neuro)developmental features including autism spectrum disorder, developmental delay, and epilepsy. Taken together, our findings implicate de novo MCM6 variants in neurodevelopmental disorders. The clinical features and functional defects related to the zinc binding residue resemble those observed in syndromes related to other MCM components and DNA replication factors, while de novo OB-fold domain missense variants may be associated with more variable neurodevelopmental phenotypes. These data encourage consideration of MCM6 variants in the diagnostic arsenal of NDD., (© 2023. The Author(s).)

Several studies have examined how the lockdown restrictions enforced to halt the spread of COVID-19 have affected adults' movement behaviors; however, there is limited information regarding the effects on youth. This study aimed to report on the lifestyle habits of adolescents during COVID-19 pandemic remote learning and explore associations between the participants' bodyweight and lifestyle behaviors. We used a cross-sectional study applied to 285 students studying in the gymnasium. The response rate was 74.21%. They completed an online questionnaire regarding lifestyle, eating habits, and nutritional status (assessed by the Body Mass Index-BMI). The study was conducted in January-February 2021. The percentage of overweight and obese was higher among boys ( p = 0.001). The mean sleep duration was 8.12 (SD:1.284) hours per night, and was higher in boys than in girls. The respondents met the physical activity (PA) recommendation for their age, based mainly on unorganized PA. The screen time was 4-6 h or more for the majority of the respondents. Dietary habits included a high consumption of comfort food, like cereals, sweets, soft drinks, snacks, and fried food, but a lower consumption of vegetables and fast food. The regression analyses showed that the BMI was correlated with the BMI of the parents and the number of hours spent in front of the computers in free time. The study identified behavioral and environmental factors that can be modified with effective strategies to reduce overweight and obesity among school students and to promote a healthy lifestyle.

Staphylococcus aureus , Streptococcus pyogenes and Enterococcus faecalis can colonize the tooth root canals, adhere to dentin walls, and frequently cause periodontitis in dogs. Bacterial periodontal diseases are common in domesticated pets, causing severe oral cavity inflammation and a strong immune response. This study investigates the antioxidant effect of a natural antimicrobial mixture (Auraguard-Ag) on the ability of S. aureus , S. pyogenes and E. faecalis to infect primary canine oral epithelial cells as well as its impact on their virulence factors. Our data show that a concentration of 0.25% Ag is sufficient to inhibit the growth of all three pathogens, whereas a concentration of 0.5% will become bactericidal. The sub-inhibitory concentration of 0.125% Ag reveals that the antimicrobial mixture can significantly reduce biofilm formation and exopolysaccharide production. The impact on these virulence factors was further translated into a significantly reduced ability to infect primary canine oral epithelial cells and restore epithelial tight junctions, with no impact on the epithelial cell viability. The post-infection inflammatory cytokines (IL-1β and IL-8) and the COX-2 mediator were also reduced both in mRNA and protein expression levels. The oxidative burst, detected upon infection, was also decreased in the presence of Ag, as our results show a significant decrease in H 2 O 2 released by the infected cells. We show that inhibition of either NADPH or ERK activity will result in a downregulation of COX-2 expression and lower levels of H 2 O 2 in infected cells. Conclusively, our study shows that natural antimicrobials reduce pro-inflammatory events, post infection, through an antioxidative mechanism that involves the downregulation of the COX-2 mediator via the inactivation of ERK in the absence of H 2 O 2 . As a result, they significantly reduce the risk of secondary bacterial infections and host oxidative stress caused by Staphylococcus aureus , Streptococcus pyogenes and Enterococcus faecalis accumulation in biofilms in an in vitro canine oral infection model.

Legionella pneumophila is responsible for causing Legionnaires' disease and Pontiac fever, also known as legionellosis. The aim of this study was to investigate the mechanistic effect of a mixture of natural antimicrobials (Citrox BCL) in preventing L. pneumophila biofilm formation and reducing its in vitro virulence. The minimum inhibitory concentrations were detected at 0.06%, and the MBC was established at 0.125%. Based on the growth curve profile, the sub-inhibitory concentration of 0.02% was further used to study the mechanistic implications in the absence of a cytotoxic effect on A549 cells. At 24 h post-infection, Citrox BCL reduced ( p = 0.005) the intracellular growth of L. pneumophila when the A549 cells or the bacteria were pre-treated with 0.02% Citrox BCL. This result was replicated when Citrox BCL was added during the 24 h infection assay leading to a reduction in intracellular growth ( p = 0.003). Herein we show that at the sub-inhibitory concentration of 0.02%, Citrox CBL lowers the ROS levels in infected A549 cells and causes a 45% reduction in L. pneumophila EPS production, a reduction associated with the decline in biofilm formation. Overall, our results corroborate the low c-di-GMP production with the decrease in biofilm formation and low EPS levels. The low EPS levels seemed to be caused by the downregulation of the tat B and tat C gene expressions. Moreover, inhibition of pvc A and pvc B gene expressions, leading to lower siderophore levels, suggests that Citrox BCL reduces the ability of L. pneumophila to sequester iron and reduce biofilm formation through iron starvation.

This narrative review presents the findings from intervention studies on the effects of sleep deprivation on eating habits, metabolic rate, and the hormones regulating metabolism, and discusses their relevance to weight loss efforts. Disturbed sleeping patterns lead to increased energy intake, partly from excessive snacking, mainly on foods high in fat and carbohydrates. The studies focused mainly on the effects of sleep duration, but also of sleep quality, on dietary intake during weight loss trials, and on weight loss maintenance. It is important to explore sleep routines that could enhance the efforts of obese and overweight people to lose weight, maintain their weight loss, and improve their overall health.

The study aimed to evaluate lifestyle factors among Information Technology (IT) workers from Romania before and during the pandemic. We used an online applied questionnaire, filled in by 1638 respondents, that assessed nutrition status-Body Mass Index (BMI), weight and diet change, physical activity, alcohol consumption, number of hours working in front of the computer, stress, and sleep. Multivariate logistic regression was used to establish the lifestyle factors that lead to weight gain. Although the level of physical activity (PA) was low before the pandemic, the results of our study showed a further decrease in physical activity. In total, 61.1% of men and 71.1% of women performed PA for less than 30 min per day. Weight gain was reported in 50.5% of men and 45.3% of women (mean weight gain was 5.11 ± 3.52 kg) as a result of increased screen time (with a mean of 3.52 ± 4.29 for females and 3.05 ± 2.09 for males, p > 0.05 h per day) and the reported changes in diet. Despite the popularity of home-cooked foods, the intake of vegetables and fruit remained low. The quality of sleep was poor for 55.7% of the respondents. Public health and corporation policies are required to encourage a healthy lifestyle and avoid chronic diseases.

Air pollution has become the most important issue concerning human evolution in the last century, as the levels of toxic gases and particles present in the air create health problems and affect the ecosystems of the planet. Scientists and environmental organizations have been looking for new ways to combat and control the air pollution, developing new solutions as technologies evolves. In the last decade, devices able to observe and maintain pollution levels have become more accessible and less expensive, and with the appearance of the Internet of Things (IoT), new approaches for combating pollution were born. The focus of the research presented in this paper was predicting behaviours regarding the air quality index using machine learning. Data were collected from one of the six atmospheric stations set in relevant areas of Bucharest, Romania, to validate our model. Several algorithms were proposed to study the evolution of temperature depending on the level of pollution and on several pollution factors. In the end, the results generated by the algorithms are presented considering the types of pollutants for two distinct periods. Prediction errors were highlighted by the RMSE (Root Mean Square Error) for each of the three machine learning algorithms used.

Alcohol consumption is a major public health problem facing universities. The objectives of the present study were to investigate alcohol consumption and the behaviors associated with it among Romanian university medical students, with particular reference to sex differences, behavioral consequences, and lifestyle patterns. We performed a cross-sectional study on 722 medical students (60.4% females; 39.6% males); the participants filled out a validated questionnaire containing the following items co-occurring with alcohol consumption: smoking, illicit drug use, energy drink consumption, and other behavioral drinking consequences. Physical activity was estimated using the IPAQ questionnaire. We statistically analyzed the interrelation between alcohol consumption and target factors. The present study showed a high percentage of at-risk drinkers among male (15.0%) and female medical students (14.9%) in the studied group. Male students reported higher illicit drug use and physical activity than female students, but the at-risk female drinkers' group consumed more drugs than the low-risk female drinkers. Both male and female drinkers engaged in other risky behaviors correlated with drinking (e.g., smoking, low academic performance, and driving a car after drinking). Public health policies, strategies, and interventions should be initiated to reduce alcohol consumption and associated behaviors in medical students.

Background: Migration of healthcare workers is receiving increased attention worldwide. In Europe, the creation of a border-free labor market and its expansion with the EU enlargements of 2004, 2007, and 2013 endowed health professionals with the right to provide services and to relocate to another EU Member State. For the Romanian doctors, the EU-wide recognition of the medical degree obtained in Romania has created new opportunities, while inadequate working conditions and relatively low salaries pushed many of them to search for employment abroad. As there is considerable uncertainty about the magnitude of the Romanian physicians' exodus, we performed a survey to assess the emigration intention of future Romanian doctors., Methods: The study was conducted over three consecutive years: 2013, 2014, and 2015 at the University of Medicine and Pharmacy "Iuliu Hatieganu" Cluj-Napoca, Romania. The self-administrated questionnaire included 19 questions regarding students' emigration intentions., Results: All the 957 license-degree students participated in the study. In this study, 84.7% of subjects planned on seeking employment abroad after graduation. A large number of the students who have participated in the study have already started preparing for emigration, 21.7% of those who wished to migrate had already performed at least one Erasmus mobility in their country of choice, 44.5% have been enrolled in a language course, and 42.7% have searched for jobs on the Internet., Conclusions: The majority of Romanian medical students considering migration see it as a serious alternative to the continuation of their professional training started in Romania. The findings of this study are upsetting and can impact both policy crafting and future research. Structural reforms in the healthcare provisions are needed in order to facilitate the retention of medical personnel. Romanian policy makers need to devise a comprehensive national health workforce plan to deal with physician migration.

Background: Stigmatizing attitudes to mental illness, and especially schizophrenia, are not limited to the general population but are also common among health professionals. Health professionals are in a position to model health related attitudes both in the general public and patients. Medical students are an interesting group to focus upon, since they are future health professionals and correcting stigmatizing attitudes is still possible during their educational curriculum., Methods: This study investigated the attitude toward mental illness in medical students at the Iuliu Hatieganu University of Medicine and Pharmacy. We surveyed first year students, since they have not yet received specific classes or internships in psychiatry; 322 students from the Romanian and English sections participated, representing a response rate of 94.7%. The questionnaire consisted of the Romanian and English versions of Link's Social Distance Scale towards people with mental illness scale., Results: Overall, medical students had a relatively negative attitude towards people with mental illness, with moderate social distance and stereotypical attitudes. The level of personal contact with people with mental illness was correlated with positive attitudes. International students had scored lower then Romanian students on social distance toward mentally ill patients., Conclusions: Medical education can play an important role in the attitudes of students toward mental illness. Medical students have stigmatizing attitudes about mentally ill patients. Personal contact with people suffering from mental illness might contribute to a positive attitude from the medical students toward mentally ill patients.

Background: Alcohol/binge drinking among university students has become a major public health problem. Many of young students will be exposed to substantial changes in living arrangements, socialization groups and social activities during the transitional period., Aim: The aim of this study was to analyse the alcohol consumption in Romanian university students, and to describe the behaviours occurring after drinking., Methods: A cross-sectional study was conducted on 468 undergraduate students, from a university for medicine and law. Of these students, 35.5% were males and 64.5% were females. The mean age of students was 21.9 ± 3.22 years. Validated anonymous paper questionnaires were completed voluntary by the students. Questionnaires contained demographic items, six questions for determining the level of alcohol consumed in terms of quantity and frequency, and 19 statements or problems resulting from drinking., Results: The findings of the study showed that males drunk more units of alcohol/week than females (p<0.001). The prevalence of abstainers was 10.8% in males and 17.6% in women. Heavy drinkers (drinking 5 or more drinks more than once a week) were more common among male (19.3%) than among female students (16.2%). Most frequently, drinking behaviours are related to academic performance, and the possible link between poor academic performance and alcohol consumption appears tenuous and merits further investigation., Conclusion: Effective intervention strategies should be implemented to prevent students' alcohol consumption and adverse health and social consequences resulting from this behaviour.

Introduction: Anatomy is an important and intense mandatory course offered during the first year of medical school. Corpse dissection is very important in Anatomy teaching, and first year students will encounter, most likely for the first time, a dead human body during Anatomy labs., Objectives: The purpose of this study was to evaluate the anxiety experienced by medical students with no previous corpse dissection background just before, after a week, and then after a month of dissection labs, and to investigate the relationship between students' personality and their attitude towards dissection., Subjects and Methods: 138 first year English Section medical students from the "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania, completed a personality inventory, an anxiety scale and a questionnaire to assess their attitudes and reactions to anatomical dissection., Results: The level of anxiety reported by students increased from before the first dissection encounter to after one month of dissection labs., Conclusions: There is a relationship between the Five Factor model of personality and students' attitudes towards dissection. Medical students could be better prepared for their first corpse dissection experience if the preparation before dissection would take in consideration their psychological traits.

Aim: Our objective was to evaluate the attitudes and views of first year medical students towards cadaver dissection in anatomy learning and discuss various findings in relation with ethical problems)., Material and Method: The study was conducted at the "Iuliu Hat ieganu" University of Medicine and Pharmacy, during the academic year 2012-2013 at the end of the second semester. There were 121 first year medical students included. We developed a questionnaire to asses among other, the degree of fear, anxiety and stress in the dissection room, methods of coping, ethical aspects of dissection and hand it to the students., Results: 34.7% of students experienced different levels of fear on exposure to the dissection room practical sessions. Many students experienced anxiety in reaction to dissection. In the first semester most students reported physical and behavioral reaction towards certain stimuli, with a decrease in the second semester. Recurring visual images of cadavers, reported by 57% of students in the first semester, dropped to 44.6% in the second semester. Students used most frequently the "rationalization and emotional detachment" as a coping method., Conclusions: Anatomists, most often the firsts who need to be aware of emotional and ethical issues, need to explain in detail the steps necessary for dissection and that dissection is performed with the respect of legislation, ethics and human rights.

We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5'-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction phase microscopy together with a Fourier transform light scattering technique. RBC cytosolic ATPs are both chemically and metabolically depleted, and the corresponding scattering signals are compared with the light scattering signal of normal RBCs having physiologic levels of ATP.

Microfluidic tools are providing many new insights into the chemical, physical and physicochemical responses of cells. Both suspension-level and single-cell measurements have been studied. We review our studies of these kinds of problems for red blood cells with particular focus on the shapes of individual cells in confined geometries, the development and use of a 'differential manometer' for evaluating the mechanical response of individual cells or other objects flowing in confined geometries, and the cross-streamline drift of cells that pass through a constriction. In particular, we show how fluid mechanical effects on suspended cells can be studied systematically in small devices, and how these features can be exploited to develop methods for characterizing physicochemical responses and possibly for the diagnosis of cellular-scale changes to environmental factors.

Background: Legal and illegal circulatory migration from Romania reached huge proportions after 2000, following the lifting of the visa requirements for EU Shengen countries. So far, the impact of migration on health has received scarce attention from Romanian authorities., Aims of the Study: To describe the socio-demographic and clinical profile of the migrants who have developed mental illness, estimate their services use in terms of hospitalization and to analyze the cost impact on the Romanian health system and on the migrants' co-payments, to discuss the possible relationships between migration and mental health., Methods: A semi-structured interview, designed by the authors, has been administered to 50 migrants admitted to the Second Psychiatric Clinic Cluj-Napoca, Romania, to investigate the following areas: immigration status, working conditions, income, housing, insurance and social bonds. The clinical symptomatology of these patients was assessed using the Brief Psychiatric Rating Scale (BPRS). The average cost of hospitalization per day per patient, the total costs of hospitalization and the migrants' co-payment through personal contribution to the insurance system were estimated., Results: Most of the patients were young, single, with no previous experience abroad and with few social ties in the host country, with unqualified and insecure jobs. In this group, 45 out of 50 had schizophrenia spectrum disorders diagnoses. The hospitalization length of these patients was slightly shorter than the hospitalization of non-migrant patients with the same diagnosis. Individuals from rural areas had longer hospitalisation than those coming from urban areas. Those who left the country illegally and those who worked illegally had shorter hospitalisations. The average costs of hospitalization per day per patient were Euro 15.56; and the total costs were Euro 14,054.92. In order to cover the costs of hospitalization in the native country due to an illness with the onset abroad, a patient should work and contribute 4.65 years (on the basis of minimum salary) as a co-payment for the hospitalization in the native country., Discussion: The patients considered in the present study fit the general profile of the emigrants. Discrimination, social isolation, insecurity may increase the risk for mental illness. A relevant role in the length of hospitalization may be due to the urban/rural dimension: patients from rural areas have less access to mental health care, therefore when they are finally hospitalised, they may remain longer because, in case of relapse, rapid readmission may not be possible. The shorter stay in hospital for those who left the country and worked illegally may be due to better resistance to stress and flexibility, to an illness that was not in an advanced phase, or to inability to afford higher personal co-payment for longer hospitalizaton., Implications for Health Policies: More accurate and careful screening for mental illness should be applied at least for legal emigrants. Cultural adjustment programs should be organized prior to departure. Contact and counseling points in the host country would be important for prevention and treatment of mental illnesses. Programs focused on circulatory migrants and aimed at developing work opportunities in the native country would increase their self confidence and enable them to perceive their return as success and not as failure.

Using a novel noncontact technique based on optical interferometry, we quantify the nanoscale thermal fluctuations of red blood cells (RBCs) and giant unilamellar vesicles (GUVs). The measurements reveal a nonvanishing tension coefficient for RBCs, which increases as cells transition from a discocytic shape to a spherical shape. The tension coefficient measured for GUVs is, however, a factor of 4-24 smaller. By contrast, the bending moduli for cells and vesicles have similar values. This is consistent with the cytoskeleton confinement model, in which the cytoskeleton inhibits membrane fluctuations [Gov et al., Phys. Rev. Lett. 90, 228101, (2003).