1. Influence of CYP2C19 on Helicobacter pylori eradication in Brazilian patients with functional dyspepsia
- Author
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E F Nardelli, Débora Dreher Nabinger, Guilherme Becker Sander, Rafael do Amaral Cristovam, Daniel Simon, Mirelli Gabardo Klein, G S de Moraes, Carlos Fernando de Magalhães Francesconi, M C Osório, L. Basso da Silva, Felipe Mazzoleni, Tássia Flores Rech, and Luiz Edmundo Mazzoleni
- Subjects
Male ,Endoscopia gastrointestinal ,Functional dyspepsia ,Genótipo ,Gastroenterology ,Endoscopy, Gastrointestinal ,Cytochrome P450 CYP2C19 ,0302 clinical medicine ,Clarithromycin ,Infecções por Helicobacter ,Inativação metabólica ,Genotype ,030212 general & internal medicine ,Omeprazole ,Polimorfismo de nucleotídeo único ,education.field_of_study ,biology ,General Medicine ,Middle Aged ,Inactivation, Metabolic ,Female ,030211 gastroenterology & hepatology ,Brazil ,Helicobacter pylori eradication ,medicine.drug ,Adult ,medicine.medical_specialty ,Population ,Poor metabolizer genotype ,Rapid urease test ,CYP2C19 ,Polymorphism, Single Nucleotide ,Helicobacter Infections ,03 medical and health sciences ,Internal medicine ,Genetics ,medicine ,Humans ,Dyspepsia ,education ,Molecular Biology ,Dispepsia ,Aged ,Helicobacter pylori ,business.industry ,Brasil ,Amoxicillin ,biology.organism_classification ,Cytochrome P-450 CYP2C19 ,business - Abstract
The aim of this study was to examine the effect of polymorphisms in the cytochrome P450 (CYP) 2C19 gene (CYP2C19) on the Helicobacter pylori eradication rate in Brazilian patients with functional dyspepsia. Adults diagnosed with functional dyspepsia based on the ROME III criteria and infected with H. pylori were recruited to this study. The patients were subjected to gastrointestinal endoscopy and the H. pylori status was defined when both urease test and histopathology results were negative or positive. The patients were treated with proton pump inhibitor-based triple therapy (omeprazole, amoxicillin, and clarithromycin). CYP2C19*2 and CYP2C19*3 were genotyped by polymerase chain reaction-restriction fragment length polymorphism. One hundred and forty-eight patients (81.8% women) with a mean (± SD) age of 46.1 (12.2) years were included in this study. Based on the CYP2C19 genotypes, the patients were classified as homozygous extensive metabolizer (HomEM; 67.6%), heterozygous extensive metabolizer (HetEM; 26.3%), or poor metabolizer (PM; 6.1%). The H. pylori eradication rates in patients with HomEM, HetEM, and PM were 85.0, 89.7, and 100.0% (P = 0.376), respectively. The included study population comprised a high frequency of patients carrying the HomEM genotype. Although the genotypes of CYP2C19 variants were not statistically significant, the results of this study suggest a possible effect of the PM genotype on the efficacy of H. pylori eradication.
- Published
- 2016
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