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15 results on '"Poofery, Juthathip"'

7. Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand

Author

Poofery, Juthathip, primary, Narapakdeesakul, Duriyang, additional, Riana, Elizabeth, additional, Arnuphapprasert, Apinya, additional, Nugraheni, Yudhi Ratna, additional, Ngamprasertwong, Thongchai, additional, Wangthongchaicharoen, Monsicha, additional, Soisook, Pipat, additional, Bhodhibundit, Phanaschakorn, additional, and Kaewthamasorn, Morakot, additional

Published
2021
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9. Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand.

Author

Poofery, Juthathip, Narapakdeesakul, Duriyang, Riana, Elizabeth, Arnuphapprasert, Apinya, Nugraheni, Yudhi Ratna, Ngamprasertwong, Thongchai, Wangthongchaicharoen, Monsicha, Soisook, Pipat, Bhodhibundit, Phanaschakorn, and Kaewthamasorn, Morakot

Subjects
*BARTONELLA, *GENETIC variation, *BARTONELLA henselae, *CITRATE synthase, *SPECIES, *ROOSTING, *Q fever
Abstract

The study of bacterial zoonoses has been under‐pursued despite the fact that bacteria cause the majority of zoonotic diseases, of which 70% have a wildlife origin. More Bartonella species are being identified as the cause of human diseases, and several of them have been linked to domestic and wild animals. Bats are outstanding reservoirs for Bartonella species because of their wide distribution, mobility, roosting behaviour, and long life span. Here, we carried out a PCR‐based survey on bats that were collected from 19 sampling sites in eight provinces of Thailand from February 2018 to April 2021. Bartonella infection was investigated in a total of 459 bats that belong to 24 different bat species (21 species of which had never been previously studied in Thailand). PCR diagnostics revealed that 115 out of 459 (25.5%) blood samples tested positive for Bartonella. The nucleotide identities of the Bartonella 16S rRNA sequences in this study were between 95.78‐99.66% identical to those of known zoonotic species (Bartonella ancashensis, Bartonella henselae, Bartonella bacilliformis and Bartonella australis) as well as to an unidentified Bartonella spp. In addition, the citrate synthase (gltA) and RNA polymerase‐beta subunit (rpoB) genes of Bartonella were sequenced and analyzed in positive samples. The gltA and rpoB gene sequences from Hipposideros gentilis and Rhinolophus coelophyllus bat samples showed low nucleotide identity (<95%) compared to those of the currently deposited sequences in the GenBank database, indicating the possibility of new Bartonella species. The phylogenetic inference and genetic diversity were generated and indicated a close relationship with other Bartonella species previously discovered in Asian bats. Overall, the current study demonstrates the primary evidence pointing to a potential novel Bartonella species in bats. This discovery also contributes to our current understanding of the geographical distribution, genetic diversity, and host ranges of bat‐related Bartonella. [ABSTRACT FROM AUTHOR]

Published
2022
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14. The Effect of Transfusion-Dependent Thalassemia Patient’s Serum on Peripheral Blood Mononuclear Cell Viability

Author

Patpan, Nawapong, primary, Banjerdpongchai, Ratana, additional, Tantiworawit, Adisak, additional, Poofery, Juthathip, additional, Komonrit, Phimphan, additional, Fanhchaksai, Kanda, additional, Rattanathammethee, Thanawat, additional, Chai-Adisaksopha, Chatree, additional, Rattarittamrong, Ekarat, additional, Norasetthada, Lalita, additional, and Charoenkwan, Pimlak, additional

Published
2019
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15. Genetic characterization of genes encoding the major surface proteins of Anaplasma marginalefrom cattle isolates in Thailand reveals multiple novel variants

Author

Arnuphapprasert, Apinya, Nugraheni, Yudhi Ratna, Poofery, Juthathip, Aung, Aung, Kaewlamun, Winai, Chankeaw, Wiruntita, Tasanaganjanakorn, Tanuwong, Wattanamethanont, Juntra, and Kaewthamasorn, Morakot

Abstract

Bovine anaplasmosis is a serious tick-borne disease that is responsible for economic loss worldwide. The major surface proteins (MSPs), encoded by msp1to msp5genes of Anaplasma marginale, play an important role in host-pathogen and tick-pathogen interactions. These markers have been used for genetic characterization and phylogenetic studies. Despite domestic reports concerning suspected outbreaks of anaplasmosis in Thailand, genetic analysis of A. marginalein the country remains largely limited. Therefore, we aim to investigate the infection rate of the rickettsia organism in the Anaplasmataceae family throughout five regions of Thailand and to further characterize the key genetic markers: msp1a, msp2, and msp5of A. marginale. From 2016 to 2021, we collected a total of 384 cattle blood samples across 18 provinces. Overall, the infection rate of the rickettsia organism in the Anaplasmataceae family was 46.1%. Over 65% of the positive samples were confirmed as A. marginale. We successfully obtained a total of 138 A. marginale msp1a(38), msp2(79), and msp5(21) sequences from all regions of the country. The msp1aand msp2genes exhibit a high degree of genetic diversity, while the msp5gene is highly conserved among the Thai isolates. Our findings regarding msp1acorroborated the genetic heterogeneity of A. marginalestrains in endemic regions worldwide. Additionally, we found multiple novel variants for the first time in the current nationwide survey. We found 45 tandem repeat characters of the msp1asequence. Among them, 24 characters were not shared with other countries. Collectively, we expanded the extent of genetic diversity in key markers; msp1aand msp2genes, and further confirmed the previous finding that msp5was highly conserved. The msp1aand msp2genes could be useful for the surveillance of newly introduced strains. The current data may also be useful in designing a vaccine containing potential epitopes of different antigens in the future.

Published
2023
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