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5. Influence of labor and oxytocin on in vitro leukotriene release by human fetal membranes and uterine decidua at term gestation

Author

Pasetto, Nino, Zicari, Alessandra, Piccione, Emilio, Lenti, Luisa, Pontieri, Giuseppe, and Ticconi, Carlo

Subjects
Leukotrienes -- Physiological aspects, Fetal membranes -- Physiological aspects, Oxytocin -- Research, Parturition -- Physiological aspects, Endometrium -- Physiological aspects, Health
Abstract

OBJECTIVE: The purpose of this study was to determine whether labor and oxytocin influence in vitro leukotriene release by human fetal membranes and uterine decidua at term gestation. STUDY DESIGN: All women (N = 21) were studied in an academic clinical research environment. They were delivered either vaginally or before labor by cesarean section. Tissues were incubated with calcium ionophore A23187 alone or with calcium ionophore A23187 after preincubation with either oxytocin, BW755c, or cycloheximide. Leulotrienes in culture medium were determined by high-performance liquid chromatography. Statistical analysis was performed with analysis of variance. RESULTS: Tissues obtained after vaginal delivery released significantly higher (p,0.05) quantities of leukotrienes [LTB.sub.4], [LTD.sub.4], and [delta] -6-trans- [LTB.sub.4] than did tissues obtained during cesarean section. Leukotriene release was stimulated by oxytocin and was inhibited by both BW755c and cycloheximide. CONCLUSION: These results suggest an activation of the arachidonate lipoxygenase pathway in these tissues during labor. Oxytocin could play a regulatory role in this process. (AM J OBSTET GYNECOL 1992; 166: 1500-6.)

Published
1992

8. Detection of antiphospholipid antibodies by immunostaining on thin layer chromatography plates

Author

Sorice, Maurizio, Griggi, T., Circella, A., Garofalo, Tina, Dagostino, F., D'Agostino, F., Pittoni, V., Pontieri, Giuseppe, Lenti, Luisa, and Valesini, Guido

Subjects
medicine.drug_class, Immunology, Phospholipid, Enzyme-Linked Immunosorbent Assay, HIV Infections, Monoclonal antibody, Sensitivity and Specificity, chemistry.chemical_compound, Antigen, medicine, Humans, Lupus Erythematosus, Systemic, Immunology and Allergy, Syphilis, Antigens, Immunosorbent Techniques, Phospholipids, Immunoassay, biology, Chemistry, Autoantibody, Antibodies, Monoclonal, Tlc immunostaining, Antiphospholipid Syndrome, Molecular biology, Thin-layer chromatography, Evaluation Studies as Topic, Antibodies, Anticardiolipin, Antibodies, Antiphospholipid, biology.protein, immunostaining, thin layer chromatography, β2-glycoprotein i, anti-cardiolipin antibody, beta(2)-glycoprotein i, antiphospholipid antibody, anti-phospholipid antibody, anticardiolipin antibody, Chromatography, Thin Layer, Antibody, Immunostaining
Abstract

There is increasing interest in the role of antiphospholipid antibodies in the so-called ‘antiphospholipid antibody syndrome’ (APS). The two major methods currently employed for detecting the autoantibodies are the solid phase ELISA and the LAI test (inhibition of phospholipid dependent coagulation assay). In our study we have tested the possibility of detecting antiphospholipid antibodies by immunostaining on thin layer chromatography (TLC) plates, since this technique permits the use of pure phospholipid molecules as antigen. Sera were collected from 20 patients with SLE without APS, 20 patients with APS, 20 anti-HIV positive subjects, ten patients with signs of APS but antiphospholipid negative (ELISA), 20 patients with syphilis and 40 matched blood donors. Results showed that only 72.3% of sera containing detectable levels of aCL antibodies in solid phase ELISA were also positive for aCL in TLC immunostaining; these discrepancies may be due to the presence of antibodies reacting with a protein complexed with phospholipid (β2-glycoprotein-I) or, alternatively, to the different antigenic presentation of phospholipids on chromatograms compared to the surface of microtitre wells. Furthermore, aCL monoclonal antibody CAL-3, as well as nine sera positive for aCL, also reacted with PS and PE. Previous absorption of these sera with CL micelles completely abolished the reactivity with PS and PE, demonstrating cross-reactivity among these three phospholipids. In conclusion, our findings reveal that TLC immunostaining is more specific, but less sensitive, than ELISA for the detection of antiphospholipid antibodies in human sera.

Published
1994
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9. L’Edema

Author

Pontieri, Giuseppe and Misasi, Roberta

Published
2010

12. Association between GM3 and CD4-Ick complex in human peripheral blood lymphocytes

Author

Sorice, Maurizio, Garofalo, Tina, Misasi, Roberta, Longo, Agostina, Mikulak, J., Dolo, V., Pontieri, Giuseppe, and Pavan, Antonio

Subjects
CD4-Positive T-Lymphocytes, gm3, cd4, gangliosides, microdomains, Microscopy, Confocal, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), CD4 Antigens, G(M3) Ganglioside, Humans, Precipitin Tests, Cell Line
Abstract

The aim of this study was to further elucidate our previous observation on molecular interaction of GM3, CD4 and p56Ick in microdomains of human peripheral blood lymphocytes (PBL). We analyzed GM3 distribution by immunoelectron microscopy and the association between GM3 and CD4-p56Ick complex by scanning confocal microscopy and co-immunoprecipitation experiments. Scanning confocal microscopy analysis showed an uneven signal distribution of GM3 molecules over the surface of human lymphocytes. Nearly complete colocalization areas indicated that CD4 molecules were distributed in GM3-enriched plasma membrane domains. Co-immunoprecipitation experiments revealed that CD4 and p56Ick were immunoprecipitated by IgG anti-GM3, demonstrating that GM3 tightly binds to the CD4-p56Ick complex in human PBL. In order to verify whether GM3 association with CD4 molecules may depend on the presence of p56Ick, we analyzed this association in U937, a CD4 + and p56Ick negative cell line. The immunoprecipitation with anti-GM3 revealed the presence of a 58kDa band immunostained with anti-CD4 Ab, suggesting that the GM3-CD4 interaction does not require its association with p56Ick. These findings support the view that GM3 enriched-domains may represent a functional multimolecular complex involved in signal transduction and cell activation.

Published
2001

15. IFN gamma and TNF alpha cause an increased release of C3 by murine macrophages

Author

DI RENZO, Livia Maria, DE CRISTOFARO MR, Zicari, Alessandra, Viola, R, Pontieri, Giuseppe, and Lipari, Marcella

Subjects
Male, Tumor Necrosis Factor-alpha, Enzyme-Linked Immunosorbent Assay, Complement C3, Recombinant Proteins, Mice, Inbred C57BL, Interferon-gamma, Mice, Culture Media, Conditioned, Macrophages, Peritoneal, Animals, Interleukin-2, Female, Cells, Cultured, Spleen
Abstract

Macrophages from mice bearing Lewis lung carcinoma release higher amounts of C3 molecules than macrophages from healthy mice. The C3 pro-releasing activity operating in vivo was suspected to be due to an immunological network. Indeed, the supernatants of splenocytes from tumor bearing mice, but not from normal mice, induced in vitro an increased release of C3 molecules by macrophages. Recombinant IFN gamma and TNF alpha were strong inducers of C3 release, while IL-2 acted poorly. The C3 pro-releasing activity of splenocyte supernatants was largely prevented by their pretreatment with specific mAb anti IFN gamma or anti TNF alpha, but not completely prevented by the simultaneous neutralization of the two cytokines. Taken together, these results show that murine macrophages increase the release of C3 molecules upon treatment with IFN gamma or TNF alpha and that these cytokines released in vivo by splenocytes from tumor bearing mice may account, together with a yet unknown factor, for a humoral network causing the increased release of C3 by peritoneal macrophages.

Published
1994

16. Ganglioside expression in human pancreatic islets

Author

Dotta, Franscesco, Colman, Peter G., Lombardi, Daniela, Scharp, David W., Andreani, Domenico, Pontieri, Giuseppe, Di Mario, Umberto, Lenti, Luisa, Eisenbarth, George S., and Nayak, Ramesh C.

Subjects
Diabetes -- Development and progression -- Physiological aspects, Gangliosides -- Measurement -- Analysis -- Physiological aspects, Glycolipids -- Measurement -- Physiological aspects -- Analysis, Islet cell stimulating antibodies -- Analysis -- Physiological aspects -- Measurement, Health, Physiological aspects, Analysis, Development and progression, Measurement
Abstract

A large body of experimental evidence indicates that insulin-dependent (type 1) diabetes mellitus is an organ-specific autoimmune disease (1). The autoimmune attack appears to be directed at the Beta-cells of [...]

Published
1989

17. C3-induced 3LL cell proliferation is mediated by C kinase

Author

Longo, Agostina, primary, Gradini, Roberto, additional, Mattei, Vincenzo, additional, Morgante, Emanuela, additional, Sale, Patrizio, additional, Tafani, Marco, additional, Lipari, Marcella, additional, Pontieri, Giuseppe M., additional, and Russo, Matteo A., additional

Published
2005
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