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1. Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses.

Author

Auewarakul P, Sangsiriwut K, Chaichoune K, Thitithanyanont A, Wiriyarat W, Songserm T, Ponak-nguen R, Prasertsopon J, Pooruk P, Sawanpanyalert P, Ratanakorn P, and Puthavathana P

Subjects
Humans, Influenza, Human epidemiology, Influenza, Human virology, Population Surveillance methods, Genome, Viral, Influenza A Virus, H5N1 Subtype genetics, Influenza A Virus, H5N1 Subtype isolation & purification, Reassortant Viruses genetics, Reverse Transcriptase Polymerase Chain Reaction methods
Abstract

Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex reverse transcription-PCR assay that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate surveillance for a virus resulting from reassortment between human influenza virus and the H5N1 virus.

Published
2007
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