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Abstract Background Data on sensorineural deafness (CSD) in purebred white client-owned cats is limited as most of the information on this disease entity is assured from mixed-breed experimental colonies. It is known that cats with blue irises are more predisposed to CSD having been described as a condition in which many structures in the inner ear are damaged resulting in hearing loss. Cats with CSD are born deaf or lose their hearing irreversibly within the first 4-5 weeks of life. It is important to diagnose cats with this hereditary condition in order to eliminate affected individuals from breeding. The objectives of this study were to ensure data on prevalence of CSD in a population of 72 client-owned purebred white cats in Poland according to the color of the irises and to determine if there are any predispositions with regard to CSD among different breeds of cats in which the dominant W gene is present. Results Conducted study included 72 purebred white cats from six different breeds. The prevalence of CSD in the conducted study was 16.7%, CI95 [8.9%; 23.3%]. Unilateral deafness (11.1%, CI95 [4.9%; 20.7%]) was more common than bilateral CSD (5.6%, CI95 [1.5%; 13.6%]). The studies did not show any association between sex and CSD, p = .46. No association between the blue color of irises and deafness in the studied population could be found, p = .91. When compared to the rest of the examined population, no association was found between CSD and a particular breed. Conclusions Overall prevalence of CSD regarding the examined population of purebred client-owned cats was reported as lower when compared to previous studies concerning purebred cats. Cats with blue irises are more likely to be deaf in accordance to the current state of knowledge, however in the conducted study, no significant association between the presence of blue irises and deafness in white purebred cats could be identified. In order to eliminate CSD from the population, it is necessary to conduct examinations and diagnose CSD in white cats with blue irises as well as with irises of color other than blue. Association between particular breed and CSD wasn’t identified.

In canine and feline populations, the number of neoplasm cases continues to increase around the world. Attempts are being made in centres of research to identify new biomarkers that speed up and improve the quality of oncological diagnostics and therapy in human and animal tumour patients. Cyclooxygenase-2 (COX-2) is a promising biomarker with increasing relevance to human oncology, but as yet with less application in veterinary oncology. The expression of COX-2 increases significantly during pathological processes involving inflammation, pain or fever. It is also overexpressed in humans presenting various types of tumours and in selected types of tumours in animals, particularly in dogs. This article discusses the expression of COX-2 in canine and feline tumours, the importance of COX-2 as a biomarker with diagnostic, therapeutic, prognostic and predictive relevance in oncology, and the clinical significance of inhibiting COX-2 overexpression in tumours.

The cyclooxygenase-2 (COX-2) enzyme catalyses the first stage of biosynthesis of prostanoids, proteins that are implicated in various physiological and pathological processes in humans and animals. The expression of COX-2 increases significantly during pathological processes accompanied by inflammation, pain and fever. Overexpression of COX-2 was determined in tumour tissues, which suggests that this enzyme participates in oncogenesis. In this paper the topics discussed are mechanisms regulating COX-2 expression, COX isoforms, their role in the body and the oncogenic mechanisms triggered by the overexpression of COX-2, including inhibition of apoptosis, intensification of neoangiogenesis, increased metastatic capacity, and weakening of the immune system. The significance of and the mechanisms by which COX-2 participates in oncogenesis have been studied intensively in recent years. The results are highly promising, and they expand our understanding of the complex processes and changes at the molecular, cellular and tissue level that promote oncogenesis and cancer progression. Notwithstanding the knowledge already gleaned, many processes and mechanisms have not yet been elucidated in human medicine and, in particular, in veterinary medicine. Further research is required to develop effective tumour diagnostic methods and treatment procedures for humans and animals.

Abstract Although the European flounder is frequently used in research and has economic importance, there is still lack of comprehensive transcriptome data for this species. In the present research we show RNA-Seq data from ten selected organs of P. flesus female inhabiting brackish waters of the Gulf of Gdańsk (southern Baltic Sea). High throughput Next Generation Sequencing technology NovaSeq 6000 was used to generate 500 M sequencing reads. These were mapped against European flounder reference genome and reads extracted from the mapping were assembled producing 61k reliable contigs. Gene ontology (GO) terms were assigned to the majority of annotated contigs/unigenes based on the results of PFAM, PANTHER, UniProt and InterPro protein databases searches. BUSCOs statistics for eukaryota, metazoa, vertebrata and actinopterygii databases showed that the reported transcriptome represents a high level of completeness. The data set can be successfully used as a tool in design of experiments from various research fields including biology, aquaculture and toxicology.

Nine dogs with Chiari malformation and syringomyelia, which were subjected to low-field magnetic resonance imaging (MRI) test, were described. The results of MRI examinations were presented. The outcomes of pharmacological therapy involving two dogs and surgical treatment of one dog were also described. The applied treatments produced positive short-term outcomes, and they eliminated the clinical symptoms of the disease.

Abstract Background Data regarding congenital sensorineural deafness (CSD) in client‐owned, white Devon Rex cats is limited because most of the information on this disease comes from experiments on mixed‐breed cats. Objectives Provide data on the occurrence of CSD in a population of client‐owned purebred white Devon Rex cats. Animals Forty client‐owned, purebred, white Devon Rex cats examined at 2 different facilities. Median age of the examined cats was 19 weeks. Methods Hearing status was defined by use of brainstem auditory evoked responses. Results The occurrence of sensorineural deafness in the studied population of Devon Rex cats was estimated at 10%. Unilateral and bilateral deafness occurred equally often, with 2 individuals having each (ie, 5.0%). No association between the occurrence of CSD and sex could be found, χ2 (1, n = 40) = 0.001 (P > .99). No association between blue irises and deafness was noted in the studied population, χ2 (1, n = 40) .99). Conclusions The occurrence of CSD in a population of client‐owned, white Devon Rex cats was found to be lower compared with data obtained in previously conducted studies of deafness in purebred cats. In the studied population of Devon Rex cats, no association between blue irises and CSD was found.

The operational building data presented in this paper has been collected from six office rooms located in an office building (research and educational purposes) located on the main campus of Aalborg University in Denmark. The dataset consists of measurements of occupancy, indoor environmental quality, room-level and system-level heating, ventilation and lighting operation at a 5 min resolution. The indoor environmental quality and building system data were collected from the building management system. The occupancy level in each monitored room is established from the computer vision-based analysis of wall-mounted camera footage of each office. The number of people present in the room is estimated using the YOLOv5s image recognition algorithm. The present dataset can be used for occupancy analysis, indoor environmental quality investigations, machine learning, and model predictive control.

In this study, the effect of pre-slaughter handling on the content of macro- and micronutrients in blood serum and in the breast muscle of turkeys was assessed. Four different variants of pre-slaughter handling were used in the research: no transport (N-T), transport for a distance of 100 km (T-100), transport for a distance of 200 km (T-200), and transport for a distance of 300 km (T-300). In each of them, 30 female and 30 male turkeys were used. Blood was collected from the birds before slaughter, and samples of the pectoral muscle were collected after slaughter. In the obtained biological material, the content of Mg, P, Ca, Fe, Na and K was analyzed. On the basis of the obtained research results, it can be concluded that the pre-slaughter handling negatively affects the content of macro- and microelements both in the blood serum and in the pectoral muscles of experimental turkeys. Additionally, differences due to the sex of birds were observed.

A Project of Changes to Civil Procedure in the Works of the Civil Reform Committee (1814–1815). A Source Edition – Part 1 (Trial) The present source publication is the fifth in a series encompassing the most important documents related to preparations for the replacement of French codes with national codification written by the Civil Reform Committee which worked between 1814 and 1815. Herein are contained the minutes of the sessions held by the Committee which concern a key debate among its members who argued as to whether to derogate the French Code of Civil Procedure in its entirety or only in fragments which were considered to be the most burdensome in Polish realities. Moreover, the publication also includes the first part of “The project of the civil judicial procedure in first instance”(primary procedure) presented by Franciszek Grabowski, a preeminent traditionalist in the Committee, along with fragments of the minutes reporting the course of the discussion concerning the project. Together with the second part of the proposition submitted by Grabowski encompassing enforcement proceedings, a counterproject of the code of civil procedure by progressive judge Antoni Bieńkowski, as well as a debate over these two proposals, which are to be published in the next issues of “Krakowskie Studia z Historii Państwa i Prawa”, the materials presented below portray the crux of the debate held over the Code de procedure civile of 1806, which aroused much controversy, and which was in fact the most criticized part of the French legislation in force in Polish territories. * Niniejsza publikacja została przygotowana w ramach projektu „Kodyfikacja narodowa – fantazmat czy realna alternatywa? W kręgu debat nad rodzimym systemem prawa sądowego w konstytucyjnym Królestwie Polskim” finansowanego ze środków Narodowego Centrum Nauki na podstawie umowy nr UMO-2015/18/E/HS5/00762.

The data presented were sourced from 34,884 commercial smart heat meters and 10,765 commercial smart water meters, spanning a timeframe of up to 5 years (2018–2022). All data primarily originated from single-family houses in Aalborg Municipality, Denmark. Furthermore, comprehensive building characteristics were collected for each building, where available, from the Danish Building and Dwelling Register (BBR) and Energy Performance Certificate (EPC) input data. This effort yielded an extensive pool of up to 86 distinct characteristics per building. All smart meter data were processed employing a well-established methodology, resulting in equidistant hourly data without any erroneous or missing values. The building characteristics derived from the EPCs were additionally filtered using rule sets to improve the data quality. This dataset holds substantial value for researchers involved in the domains of the built environment, district heating, and water sectors.

* Tekst przygotowany w ramach projektu „Uregulowania prawne relacji pomiędzy dziedzicami a chłopami na centralnych ziemiach polskich w okresie od zniesienia poddaństwa do uwłaszczenia”, finansowanego przez Narodowe Centrum Nauki (UMO-2018/31/B/HS5/00315).

The aim of this study was to evaluate whether production costs can be reduced and whether the profitability of organic chicken fattening can be increased by selecting the appropriate genotypes. Rhode Island Red (K-11) and Sussex (S-66) conservative chicken genotypes, as well as modern chicken genotypes such as slow-growing Hubbard JA 957 hybrids and fast-growing Ross 308 hybrids, were selected for the study. One hundred and sixty chickens were used in the experiment (forty birds per group). The birds were fed commercial organic complete feed up to 52 days of age and organic farm-made feed (to reduce costs) between 53 and 81 days of age. Population distribution was determined using the Kolmogorov–Smirnov test. The results were analyzed statistically using one-way analysis of variance (ANOVA) with multiple comparisons and the post hoc Tukey test at a significance level of p < 0.05. The 81-day fattening period (the minimum recommended period in organic farming) led to considerable weight deficits in Rhode Island Red and S-66 chickens and excessive slaughter/trade weights in Hubbard JA 957 and Ross 308 chickens. The feed conversion ratio was high in Rhode Island Red (K-11) and Sussex (S-66) chickens at 4.19 and 4.50, respectively, and much lower in Hubbard JA 957 and Ross 308 chickens at 2.79 and 2.53, respectively. The choice of chicken genotypes had a major impact on the profitability of organic farming, and the total costs of feed and other ingredients per kg of body weight were determined at EUR 3.83 for Rhode Island Red (K-11), EUR 3.90 for Sussex (S-66), EUR 6.57 for Hubbard JA 957, and EUR 6.62 for Ross 308 genotypes. The profitability of organic farming can be increased by selecting modern, meat-type, slow-growing chicken genotypes.

A Project of Changes to Civil Procedure in the Works of the Civil Reform Committee (1814–1815). A Source Edition – Part 2 (Enforcement Proceedings) The present source publication is the sixth in a series encompassing the most important documents related to preparations for the replacement of French codes with national codification drafted by the Civil Reform Committee which worked between 1814 and 1815. The publication includes the second part of “The project of the civil judicial procedure in first instance”, which is comprised of enforcement proceedings presented just like the first part (the trial) by Franciszek Grabowski, and the fragments of the minutes reporting the course of the discussion concerning the solutions proposed within the project. * Niniejsza publikacja została przygotowana w ramach projektu „Kodyfikacja narodowa – fantazmat czy realna alternatywa? W kręgu debat nad rodzimym systemem prawa sądowego w konstytucyjnym Królestwie Polskim” finansowanego ze środków Narodowego Centrum Nauki na podstawie umowy nr UMO-2015/18/E/ HS5/00762.

Telomerase activity has been found in the somatic tissues of rainbow trout. The enzyme is essential for maintaining telomere length but also assures homeostasis of the fish organs, playing an important role during tissue regeneration. The unique morphological and physiological characteristics of triploid rainbow trout, when compared to diploid specimens, make them a promising model for studies concerning telomerase activity. Thus, in this study, we examined the expression of the Tert gene in various organs of subadult and adult diploid and triploid rainbow trout females. Upregulated Tert mRNA transcription was observed in all the examined somatic tissues sampled from the triploid fish when compared to diploid individuals. Contrastingly, Tert expression in the ovaries was significantly decreased in the triploid specimens. Within the diploids, the highest expression of Tert was observed in the liver and in the ovaries of the subadult individuals. In the triploids, Tert expression was increased in the somatic tissues, while the ovaries exhibited lower activity of telomerase compared to other organs and decreased compared to the ovaries in the diploids. The ovaries of triploid individuals were underdeveloped, consisting of only a few oocytes. The lack of germ cells, which are usually characterized by high Tert expression, might be responsible for the decrease in telomerase activity in the triploid ovaries. The increase in Tert expression in triploid somatic tissues suggests that they require higher telomerase activity to cope with environmental stress and maintain internal homeostasis.

Background: The European flounder is readily chosen as an experimental subject and model in physiological and ecotoxicological studies mostly because of its adaptability to laboratory conditions. Many studies utilise a quantitative PCR (qPCR) approach to ascertain the expression of target genes under experimental conditions. Such an approach relies heavily on the selection of reference genes with stable expression. Yet certain housekeeping genes are commonly used in this role, often without due consideration of their overall expression patterns. Therefore, new approaches should be developed to identify stable reference genes for a given species and to expand the general pool of genes suitable for the reference in qPCR analysis. Methods and results: Here RNA-seq data of nine flounder organs led to identify four candidate genes of the most stable expression. It was achieved by differential expression analysis and tritoconstrictor script. Specific primers were designed for the complete ORF as well as for qPCR analysis. RT-qPCR efficiencies were tested on ORF amplicon templates. Most of the genes tested showed good amplification in a wide range of template dilutions (107-101), with a correlation coefficient (R2) ranging from 0.991 to 0.998 and a consistent efficiency (E) (Sybr Green I staining and TaqMan molecular probe). Conclusions: The proposed approach based on differential expression analysis and a new bioinformatic tool is an appropriate selection method of candidates for reference genes in qPCR. The proposed approach, combining differential expression analysis with a new bioinformatics tool, provides an effective method for selecting reference gene candidates for qPCR. As a result, we can propose four genes (polr2f, yif1a, sf3b6, uba52), each with a set of validated primers, as suitable for consideration as reference genes in qPCR analysis in European flounder, an emerging model species. [ABSTRACT FROM AUTHOR]

Simple Summary: Simple Summary: Poultry can be exposed to a number of adverse stimuli during the pre-slaughter handling. Noise, movement, lack of access to water and feed, change in habitat, disruption of flock hierarchy or environmental conditions can lead to adverse changes in the bird's body. A significant increase in these factors can cause disturbances in the body's homeostasis. These changes may be reflected in meat quality. Therefore, there is a need to assess the effect of pre-slaughter turnover on meat quality. In this study, the effects of pre-slaughter treatments on changes in macro- and micronutrient content were analyzed. In this study, the effect of pre-slaughter handling on the content of macro- and micronutrients in blood serum and in the breast muscle of turkeys was assessed. Four different variants of pre-slaughter handling were used in the research: no transport (N-T), transport for a distance of 100 km (T-100), transport for a distance of 200 km (T-200), and transport for a distance of 300 km (T-300). In each of them, 30 female and 30 male turkeys were used. Blood was collected from the birds before slaughter, and samples of the pectoral muscle were collected after slaughter. In the obtained biological material, the content of Mg, P, Ca, Fe, Na and K was analyzed. On the basis of the obtained research results, it can be concluded that the pre-slaughter handling negatively affects the content of macro- and microelements both in the blood serum and in the pectoral muscles of experimental turkeys. Additionally, differences due to the sex of birds were observed. [ABSTRACT FROM AUTHOR]