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215 results on '"Poma, Paola"'

4. Vesicle-Transported Multidrug Resistance as a Possible Therapeutic Target of Natural Compounds.

Author

Rigogliuso, Salvatrice, Cusimano, Alessandra, Condorelli, Lucia, Labbozzetta, Manuela, Schiera, Gabriella, Poma, Paola, and Notarbartolo, Monica

Abstract

Background/Objectives: A key role of extracellular vesicles (EVs) is mediating both cell–cell and cell–stroma communication in pathological/physiological conditions. EVs from resistant tumor cells can transport different molecules like P-glycoprotein (P-gp), acting as a shuttle between donor and recipient cells, resulting in a phenotypic change. The aim of our work was to isolate, characterize, and inhibit the release of EVs in two multidrug resistance (MDR) cancer models: MCF-7R (breast cancer cell line) and HL-60R (acute myeloid leukemia cell line). Methods: The existence of P-gp in EVs from MDR cells was confirmed by Western blotting assays. The characterization of EVs was carried out by evaluating the size using NTA and the presence of specific markers such as CD63, Hsp70 and Syntenin. The ability of HL-60R and MCF-7R to perform horizontal transfer of P-gp via EVs to sensitive cells was assessed using three different methods. The acquisition of resistance and its inhibition in recipient cells was confirmed by MTS 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. Results: Our data showed that cell lines (MDR) release P-gp-loaded EVs, unlike sensitive cells. The acquisition of resistance determined by the incorporation of P-gp into the membrane of sensitive cells was confirmed by the reduced cytotoxic activity of doxorubicin. Natural compounds such as curcumin, lupeol, and heptacosane can block vesicular transfer and restore the sensitivity of HL-60 and MCF-7 cells. Conclusions: Our study demonstrates that natural inhibitors able to reverse this mechanism may represent a new therapeutic strategy to limit the propagation of the resistant phenotype. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Crítica de poesía hoy

Author

Junqueira, Maria Aparecida and Poma, Paola

Subjects
editorial
Abstract

Editorial of Texto Poético, issue 37 Editorial de Texto Poético 37 Editorial da Texto Poética 37

Published
2022

10. A Curcumin-BODIPY Dyad and Its Silica Hybrid as NIR Bioimaging Probes

Author

Gangemi, Chiara Maria Antonietta, primary, Salerno, Tania Maria Grazia, additional, Barattucci, Anna, additional, Cucinotta, Fabio, additional, Bonaccorsi, Paola, additional, Calabrese, Giovanna, additional, Poma, Paola, additional, Rizzo, Maria Giovanna, additional, Campagna, Sebastiano, additional, and Puntoriero, Fausto, additional

Published
2022
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14. Exploring the cellular uptake of hectorite clay mineral and its drug carrier capabilities

Author

Università degli Studi di Palermo, Notarbartolo, Monica, Massaro, Marina, Barbosa, Raquel de Melo, Emili, Carlotta, Liotta, Leonarda F., Poma, Paola, Raymo, Françisco M., Sánchez Espejo, Rita, Vago, Riccardo, Viseras Iborra, César, Riela, Serena, Università degli Studi di Palermo, Notarbartolo, Monica, Massaro, Marina, Barbosa, Raquel de Melo, Emili, Carlotta, Liotta, Leonarda F., Poma, Paola, Raymo, Françisco M., Sánchez Espejo, Rita, Vago, Riccardo, Viseras Iborra, César, and Riela, Serena

Abstract

In the last years, the use of clay minerals for pharmaceutical purposes has increased due to their interesting properties. Hectorite (Ht) is a clay belonging to the smectite group which has attracted attention for applications in biology, tissue engineering and as drug carrier and delivery system. However, the mechanisms involved in Ht cellular uptake and transport into cells, are still unclear. Herein, we used a labeled Ht (Ht/1Cl) to study both the cellular uptake, by confocal laser scanning microscopy, and internalization pathways involved in the cellular uptake, by various endocytosis-inhibiting studies and fluorescence microscopy. These studies highlighted that Ht can penetrate the cellular membrane, localizing mainly in the cytoplasm. The main intracellular transport mechanisms are the ATP-dependent ones and those where filaments and microtubules are involved. Finally, as proof of concept for the potential of Ht as carrier system, we envisaged the covalent grafting of the anticancer molecule methotrexate (MTX), chosen as model, to obtain the Ht-MTX nanomaterial. The covalent linkage was confirmed by several techniques and the morphology of the obtained nanomaterial was imaged by SEM and TEM investigations. The kinetic release of the drug from the Ht-MTX nanomaterial in physiological conditions was studied as well. Furthermore, cytotoxic studies on different cell lines, namely, HL-60, HL-60R, MCF-7, 5637, UMUC3 and RT112 showed that Ht could be a promising material for anticancer therapy.

Published
2022

18. Editorial

Author

Gontijo Rosa, Carlos, primary, Silva, Rosely de Fátima, additional, Poma, Paola, additional, and Simas, Mônica Muniz de Souza, additional

Published
2021
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46. Altered Yin Yang 1/RAF-1 kinase inhibitory protein ratio as a possible molecular marker and therapeutic target in hepatocellular carcinoma

Author

NOTARBARTOLO DI VILLAROSA, Monica, GIANNITRAPANI, Lydia, POMA, Paola, LABBOZZETTA, Manuela, FLORENA, Ada Maria, PORCASI, Rossana, MUGGEO, Vito Michele Rosario, SANDONATO, Luigi, D'ALESSANDRO, Natale, MONTALTO, Giuseppe, Notarbartolo Di Villarosa, M, Giannitrapani, L, Poma, P, Labbozzetta, M, Florena, AM, Porcasi, R, Muggeo, VMR, Sandonato, L, D'Alessandro, N, and Montalto, G

Subjects
Settore MED/12 - Gastroenterologia, Settore BIO/14 - Farmacologia, YY1,YY1AP,RKIP,HCC
Published
2010

47. Alteration of the YY1/RKIP ratio is a frequent event in hepatocellular carcinoma

Author

NOTARBARTOLO DI VILLAROSA, Monica, LABBOZZETTA, Manuela, POMA, Paola, FLORENA, Ada Maria, PORCASI, Rossana, GIANNITRAPANI, Lydia, MONTALTO, Giuseppe, D'ALESSANDRO, Natale, Cervello, M, Notarbartolo Di Villarosa, M, Labbozzetta, M, Poma, P, Florena, AM, Porcasi, R, Cervello, M, Giannitrapani, L, Montalto, G, and D'Alessandro, N

Subjects
Yin Yang 1, Raf-1 kinase inhibitor protein, Hepatocellular carcinoma, Apoptosis, Settore MED/12 - Gastroenterologia, Settore MED/06 - Oncologia Medica, Settore BIO/14 - Farmacologia
Published
2009

48. THE EFFECT OF CYP3A5 AND ABCB1 SINGLE NUCLEOTIDE POLYMORPHISMS ON TACROLIMUS DOSE REQUIREMENTS IN CAUCASIAN LIVER TRANSPLANT PATIENTS

Author

PROVENZANI A, VIZZINI G, PALAZZO U, POLIDORI P, TRIOLO F, GRIDELLI B, NOTARBARTOLO DI VILLAROSA, Monica, BIONDI, Filippo, LABBOZZETTA, Manuela, POMA, Paola, D'ALESSANDRO, Natale, PROVENZANI A, NOTARBARTOLO DI VILLAROSA, M, BIONDI F, LABBOZZETTA M, POMA P, VIZZINI G, PALAZZO U, POLIDORI P, TRIOLO F, GRIDELLI B, and D'ALESSANDRO N

Subjects
Settore MED/12 - Gastroenterologia, Settore BIO/14 - Farmacologia, CYP3A5, P-glycoprotein, SNPs, Tacrolimus, Liver transplant
Published
2008

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