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4. Brevetoxins: Toxicological Profile

Author

Brovedani, Valentina, Pelin, Marco, D’Orlando, Elisabetta, Poli, Mark, Gopalakrishnakone, P., Editor-in-chief, Haddad Jr., Vidal, editor, Tubaro, Aurelia, editor, Kim, Euikyung, editor, and Kem, William R., editor

Published
2016
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15. Quantitative detection of staphylococcal enterotoxin B by resonant acoustic profiling

Author

Natesan, Mohan, Cooper, Matthew A., Tran, Julie P., Rivera, Victor R., and Poli, Mark A.

Subjects
Staphylococcus -- Physiological aspects, Enterotoxins -- Properties, Molecular dynamics -- Research, Bioacoustics -- Research, Resonance -- Research, Chemistry
Abstract

A rapid and sensitive detection of staphylococcal entero-toxin B (SEB) was developed using a novel acoustic sensing technique: Resonant Acoustic Profiling (RAP), which utilizes high-frequency piezoelectric quartz resonators for monitoring biomolecular interactions. An automated four-channel instrument consisting of acoustic sensors covalently conjugated with anti-SEB antibodies was used. As the samples flowed across control and active sensors simultaneously, binding was measured as a change in the resonant frequency. The lower limit of detection (LLOD) for the label free direct format was 25 ng/mL. Detection sensitivity was increased by adding mass sequentially to the captured SEB on the sensor in the form of sandwich antibodies and biotin--avidin-based gold nanoparticles. The LLOD for the mass enhanced formats were 5 and 0.5 ng/mL of SEB, respectively. The lowest sensitivity corresponds to 1.3 fM in a 75 [micro]L sample. The total assay time including the enhancement steps was less than 10 min. SEB was detected in both neat urine and PBS buffer-spiked samples, with linear correlations between resonant frequency signals and SEB concentrations ([R.sup.2] of 0.999 and 0.998, respectively). No significant cross-reactivity was observed with homologue toxins SEA, SED, and TSST, but some cross-reactivity was observed with the closely related toxin [SEC.sub.1] when we used a polyclonal antibody in the assay. [SEC.sub.1] cross-reactivity was not observed when a SEB-specific monoclonal antibody was employed in the assay. Thus the specificity of the assay presented here was dependent on the quality of the antibodies used. In addition to detection, we evaluated RAP's ability to measure the toxin in unknown samples rapidly by measuring the initial binding rate of the interaction, thereby further shortening the assay time to 6 min.

Published
2009

16. Laboratory exposures to staphylococcal enterotoxin B

Author

Rusnak, Janice M., Kortepeter, Mark, Ulrich, Robert, Poli, Mark, and Boudreau, Ellen

Subjects
Research, Health aspects, Staphylococcal infections -- Health aspects -- Research
Abstract

Staphylococcal enterotoxins are 23- to 29-kDa polypeptides in the bacterial superantigen protein family. Clinical symptoms from intoxication with staphylococcal enterotoxins vary by exposure route. Ingestion results in gastrointestinal symptoms, and [...]

Published
2004

32. AOAC SMPR 2016.011:Standard Method Performance Requirements (SMPRs) for Detection of Botulinum Neurotoxins A1 and A2 in Field-Deployable, Department of Defense Aerosol Collection Devices

Author

Sharma, Shashi, primary, Arce, Jennifer, additional, Beck, Linda C, additional, Cahall, Ryan, additional, Damer, Kenneth, additional, Jackson, Paul J, additional, Lesho, Matthew, additional, Ozanich, Rich, additional, Poli, Mark, additional, Rozak, David, additional, Shah, Sanjiv, additional, Smith, Theresa, additional, Sozhamannan, Shanmuga, additional, Thirunavukkarasu, Nagarajan, additional, and Coates, Scott G, additional

Published
2017
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33. Ovatoxin-a, A Palytoxin Analogue Isolated from Ostreopsis cf. ovata Fukuyo: Cytotoxic Activity and ELISA Detection

Author

Pelin, Marco, primary, Forino, Martino, additional, Brovedani, Valentina, additional, Tartaglione, Luciana, additional, Dell’Aversano, Carmela, additional, Pistocchi, Rossella, additional, Poli, Mark, additional, Sosa, Silvio, additional, Florio, Chiara, additional, Ciminiello, Patrizia, additional, and Tubaro, Aurelia, additional

Published
2016
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39. Stereoisomers of 42-Hydroxy Palytoxin from Hawaiian Palythoa toxica and P. tuberculosa: Stereostructure Elucidation, Detection, and Biological Activities

Author

Ciminiello, Patrizia, primary, Dell’Aversano, Carmela, additional, Dello Iacovo, Emma, additional, Forino, Martino, additional, Tartaglione, Luciana, additional, Pelin, Marco, additional, Sosa, Silvio, additional, Tubaro, Aurelia, additional, Chaloin, O., additional, Poli, Mark, additional, and Bignami, Gary, additional

Published
2014
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41. An Electrochemiluminescence-Based Competitive Displacement Immunossay for the Type-2 Brevetoxius in Oyster Extracts

Author

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD, Poli, Mark A., Rivera, Victor R., Neal, Dwayne D., Baden, Daniel G., Messer, Shawn A., Plakas, Steven M., Dickey, Robert W., Said, Kathleen E., Flewelling, Leanne, Green, David, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD, Poli, Mark A., Rivera, Victor R., Neal, Dwayne D., Baden, Daniel G., Messer, Shawn A., Plakas, Steven M., Dickey, Robert W., Said, Kathleen E., Flewelling, Leanne, and Green, David

Abstract

A new competitive electrochemiluminescence-based immunoassay for the type-2 brevetoxins in oyster extracts was developed. The assay was verified by spiking known amounts of PbTx-3 into 80% methanol extracts of Gulf Coast oysters. We also provide preliminary data demonstrating that 100% acetone extracts, aqueous homogenates, and the clinical matrixes urine and serum can also be analyzed without significant matrix interferences. The assay offers the advantages of speed (2 h analysis time); simplicity (only 2 additions, one incubation period, and no wash steps before analysis); low limit of quantitation (conservatively, 50 pg/mL = 1 ng/g tissue equivalents); and a stable, nonradioactive label. Due to the variety of brevetoxin metabolites present and the lack of certified reference standards for liquid chromatography-mass spectrometry confirmation, a true validation of brevetoxins in shellfish extracts is not possible at this time. However, our assay correlated well with another brevetoxin immunoassay currently in use in the United States. We believe this assay could be useful as a regulatory screening tool and could support pharmacokinetic studies in animals and clinical evaluation of neurotoxic shellfish poisoning victims., Pub. in Journal of AOAC International, v90 n1, p173-178, 2007.

Published
2006

44. Highly Sensitive Electrochemiluminescent Nanobiosensor for the Detection of Palytoxin

Author

Zamolo, Valeria Anna, primary, Valenti, Giovanni, additional, Venturelli, Enrica, additional, Chaloin, Olivier, additional, Marcaccio, Massimo, additional, Boscolo, Sabrina, additional, Castagnola, Valentina, additional, Sosa, Silvio, additional, Berti, Federico, additional, Fontanive, Giampaolo, additional, Poli, Mark, additional, Tubaro, Aurelia, additional, Bianco, Alberto, additional, Paolucci, Francesco, additional, and Prato, Maurizio, additional

Published
2012
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46. Harmful Dinoflagellate Ostreopsis cf. ovata Fukuyo: Detection of Ovatoxins in Field Samples and Cell Immunolocalization Using Antipalytoxin Antibodies

Author

Honsell, Giorgio, primary, De Bortoli, Marco, additional, Boscolo, Sabrina, additional, Dell’Aversano, Carmela, additional, Battocchi, Cecilia, additional, Fontanive, Giampaolo, additional, Penna, Antonella, additional, Berti, Federico, additional, Sosa, Silvio, additional, Yasumoto, Takeshi, additional, Ciminiello, Patrizia, additional, Poli, Mark, additional, and Tubaro, Aurelia, additional

Published
2011
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