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31 results on '"Pohóczky, Krisztina"'

3. Transient receptor potential Ankyrin 1 Ion channel is expressed in Osteosarcoma and its activation reduces viability

Author

Hudhud, Lina, Rozmer, Katalin, Kecskés, Angéla, Pohóczky, Krisztina, Bencze, Noémi, Buzás, Krisztina, Szőke, Éva, Helyes, Zsuzsanna, Hudhud, Lina, Rozmer, Katalin, Kecskés, Angéla, Pohóczky, Krisztina, Bencze, Noémi, Buzás, Krisztina, Szőke, Éva, and Helyes, Zsuzsanna

Abstract

Osteosarcoma is a highly malignant, painful cancer with poor treatment opportunities and a bad prognosis. Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are non-selective cation channels that have been of great interest in cancer, as their expression is increased in some malignancies. In our study we aim to characterize the expression and functionality of the TRPA1 and TRPV1 channels in human and mouse osteosarcoma tissues and in a mouse cell line. TRPA1/Trpa1 and TRPV1/Trpv1 mRNA expressions were demonstrated by PCR gel electrophoresis and RNAscope in situ hybridization. The function of these channels was confirmed by their radioactive 45Ca2+ uptake in response to the TRPA1 agonist, Allyl-isothiocyanate (AITC), and TRPV1 agonist, capsaicin, in K7M2 cells. An ATP-based K2M7 cell viability luminescence assay was used to determine cell viability after AITC or capsaicin treatments. Both TRPA1/Trpa1 and TRPV1/Trpv1 were expressed similarly in human and mouse osteosarcoma tissues, while Trpa1 transcripts were more abundantly present in K7M2 cells. TRPA1 activation with 200 µM AITC induced a significant 45Ca2+ influx into K7M2 cells, and the antagonist attenuated this effect. In accordance with the lower Trpv1 expression, capsaicin induced a moderate 45Ca2+ uptake, which did not reach the level of statistical significance. Both AITC and capsaicin significantly reduced K7M2 cell viability, demonstrating EC50 values of 22 µM and 74 µM. The viability-decreasing effect of AITC was significantly but only partially antagonized by HC-030031, but the action of capsaicin was not affected by the TRPV1 antagonist capsazepine. We provide here the first data on the functional expression of the TRPA1 and TRPV1 ion channels in osteosarcoma, suggesting novel diagnostic and/or therapeutic perspectives.

Published
2024
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9. Addition of epidermal growth factor receptor inhibitors to standard chemotherapy increases survival of advanced head and neck squamous cell carcinoma patients: A systematic review and meta‐analysis.

Author

Kiss, Fruzsina, Pohóczky, Krisztina, Görbe, Anikó, Dembrovszky, Fanni, Kiss, Szabolcs, Hegyi, Péter, Szakó, Lajos, Tóth, Lilla, Ezer, Éva Somogyiné, Szalai, Eszter, and Helyes, Zsuzsanna

Subjects
*DISEASE progression, *META-analysis, *MEDICAL information storage & retrieval systems, *CONFIDENCE intervals, *EPIDERMAL growth factor receptors, *CANCER chemotherapy, *SYSTEMATIC reviews, *MORTALITY, *HEAD & neck cancer, *ANTINEOPLASTIC agents, *CANCER patients, *RESEARCH funding, *PROGRESSION-free survival, *MEDLINE, *ODDS ratio, *SQUAMOUS cell carcinoma, *OVERALL survival
Abstract

Head and neck squamous cell carcinoma (HNSCC) is among the common tumors associated with high mortality. The aim of our meta‐analysis was to determine how additional anti‐epidermal growth factor receptor (EGFR) therapy to standard chemotherapy affects the progression‐free (PFS) and overall survival (OS) of the patients, besides the most common side effects. We used CENTRAL, MEDLINE, and Embase databases until October 26, 2020, and included 13 eligible randomized controlled trials in our systematic research. The pooled hazard ratios (HR) for the main outcomes from the original data were estimated and for the other dichotomous outcomes, odds ratios (ORs) with their 95% confidence intervals (CI) were calculated. Addition of EGFR inhibitors to conventional chemotherapy significantly decreased the death and disease progression (for PFS HR: 0.68, 95% CI: 0.55–0.81, I2 = 65.5%, p = 0.005) and mortality (for OS HR: 0.83, 95% CI: 0.72–0.94, I2 = 42.3%, p = 0.076). In the EGFR inhibitor group, we revealed an increased chance of the over Grade 3 skin rashes (OR: 4.86; 95% CI: 1.52–15.49, I2 = 2.3%, p = 0.407), and all Grade skin rashes (OR: 18.32, 95% CI: 8.07–41.60, I2 = 56.6%, p = 0.032). Despite their unwanted dermatological side effects, the addition of EGFR inhibitors is recommended to be included in advanced HNSCC therapy. [ABSTRACT FROM AUTHOR]

Published
2023
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10. Discovery of novel targets in a complex regional pain syndrome mouse model by transcriptomics: TNF and JAK-STAT pathways

Author

Pohóczky, Krisztina, primary, Kun, József, additional, Szentes, Nikolett, additional, Aczél, Tímea, additional, Urbán, Péter, additional, Gyenesei, Attila, additional, Bölcskei, Kata, additional, Szőke, Éva, additional, Sensi, Serena, additional, Dénes, Ádám, additional, Goebel, Andreas, additional, Tékus, Valéria, additional, and Helyes, Zsuzsanna, additional

Published
2022
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11. Addition of epidermal growth factor receptor inhibitors to standard chemotherapy increases survival of advanced head and neck squamous cell carcinoma patients: A systematic review and meta‐analysis

Author

Kiss, Fruzsina, primary, Pohóczky, Krisztina, additional, Görbe, Anikó, additional, Dembrovszky, Fanni, additional, Kiss, Szabolcs, additional, Hegyi, Péter, additional, Szakó, Lajos, additional, Tóth, Lilla, additional, Ezer, Éva Somogyiné, additional, Szalai, Eszter, additional, and Helyes, Zsuzsanna, additional

Published
2022
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12. Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma

Author

Kiss, Fruzsina, primary, Kormos, Viktória, additional, Szőke, Éva, additional, Kecskés, Angéla, additional, Tóth, Norbert, additional, Steib, Anita, additional, Szállási, Árpád, additional, Scheich, Bálint, additional, Gaszner, Balázs, additional, Kun, József, additional, Fülöp, Gábor, additional, Pohóczky, Krisztina, additional, and Helyes, Zsuzsanna, additional

Published
2022
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14. Discovery of novel targets in a complex regional pain syndrome mouse model by transcriptomics: TNF and JAK-STAT pathways

Author

Pohóczky, Krisztina, Kun, József, Szentes, Nikolett, Aczél, Tímea, Urbán, Péter, Gyenesei, Attila, Bölcskei, Kata, Szőke, Éva, Sensi, Serena, Dénes, Ádám, Goebel, Andreas, Tékus, Valéria, and Helyes, Zsuzsanna

Subjects
Pharmacology, Tumor Necrosis Factor-alpha, Etanercept, Disease Models, Animal, Mice, STAT Transcription Factors, Ganglia, Spinal, Immunoglobulin G, Animals, Chronic Pain, Transcriptome, Complex Regional Pain Syndromes, Janus Kinases, Signal Transduction
Abstract

Complex Regional Pain Syndrome (CRPS) represents severe chronic pain, hypersensitivity, and inflammation induced by sensory-immune-vascular interactions after a small injury. Since the therapy is unsatisfactory, there is a great need to identify novel drug targets. Unbiased transcriptomic analysis of the dorsal root ganglia (DRG) was performed in a passive transfer-trauma mouse model, and the predicted pathways were confirmed by pharmacological interventions. In the unilateral L3-5 DRGs 125 genes were differentially expressed in response to plantar incision and injecting IgG of CRPS patients. These are related to inflammatory and immune responses, cytokines, chemokines and neuropeptides. Pathway analysis revealed the involvement of Tumor Necrosis Factor (TNF) and Janus kinase (JAK-STAT) signaling. The relevance of these pathways was proven by abolished CRPS IgG-induced hyperalgesia and reduced microglia and astrocyte markers in pain-associated central nervous system regions after treatment with the soluble TNF alpha receptor etanercept or JAK inhibitor tofacitinib. These results provide the first evidence for CRPS-related neuroinflammation and abnormal cytokine signaling at the level of the primary sensory neurons in a translational mouse model and suggest that etanercept and tofacitinib might have drug repositioning potentials for CRPS-related pain.

Published
2021

15. Presence and upregulation of Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin 1 (TRPA1) in translational rat endometriosis model

Author

Pohóczky Krisztina, Kajtár Béla, Maczkó Péter, Helyes Zsuzsanna, and Bohonyi Noémi

Subjects
0301 basic medicine, chemistry.chemical_classification, Endometriosis, TRPV1, medicine.disease, 03 medical and health sciences, Transient receptor potential channel, 030104 developmental biology, 0302 clinical medicine, chemistry, Downregulation and upregulation, medicine, Cancer research, Ankyrin, psychological phenomena and processes, 030217 neurology & neurosurgery
Abstract

The Transient Receptor Potential Vanilloid 1 (TRPV1) and Ankyrin 1 (TRPA1) are non-selective cation channels predominantly localized on capsaicin-sensitive sensory neurons; however both receptors have been described in non-neuronal tissues. It has been published that both receptors upregulated in peritoneal endometriosis in humans. Our research group demonstrated that TRPA1 and TRPV1 expression is elevated in human deep infiltrating endometriosis (DIE) lesions and the receptors have an estrogen-dependent expression pattern in rat endometrium. Here, we investigated the expression changes of TRPA1/V1 and the role of the capsaicin-sensitive sensory-nerve endings in a rat model of peritoneal endometriosis. Peritoneal endometriosis was surgically induced in 8-week-old female rats (n=7-7) for 2-weeks (acute condition) and 8-weeks (chronic condition). TRPA1/V1 mRNAs were quantified with quantitative polymerase chain reaction (qPCR). The expression levels were compared with the results obtained earlier from human DIE samples. The blockade of the TRPA1/V1 expressing capsaicin-sensitive nerve endings was induced with resiniferatoxin (RTX), followed by the measurement of the weight and size of the endometriosis lesions. We detected TRPV1 and TRPA1 mRNA in normal rat endometrium, their expression was not altered in sham-operated animals. In chronic, but not in acute endometriosis the expression was significantly elevated in the lesions, which results are consistent with our previous findings in human DIE. The elimination of capsaicin-sensitive nerve endings decreased the weight of the endometriosis lesions while the size of the ectopic tissue was not altered. Taken together, our results obtained from the rat endometriosis model are consistent with the previous human results, therefore this model is considered to have translational significance and it is suitable for functional analysis of the ion channels. The local, non-neuronal TRPA1 and TRPV1 might play a role in inflammation and sensory neuronal activation in endometriosis related pain, which is mediated by a broad range of pro-inflammatory molecules.

Published
2019
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16. Contributors

Author

Acharya, Tusar Kanta, Ahern, Gerard P., Al-Omari, Ammar, Ann, Jihyae, Aswin, Tanishaa, Banerjee, Anushka, Behrendt, Marc, Belvisi, Maria G., Bencze, Noémi, Bevan, Stuart, Bishnoi, Mahendra, Blumberg, Peter M., Bonvini, Sara J., Brain, Susan D., Campbell, James N., Cantero, María del Rocío, Cantiello, Horacio F., Chapman, Hugh, Charrua, A., Chung, Man-Kyo, Connolly, James, Cruz, F., Das, Nilesh Kumar, De Logu, Francesco, Dubey, Nishant Kumar, Erin, Nuray, Fallah, Hamideh P., Geppetti, Pierangelo, Goswami, Luna, Goswami, Chandan, Halder, Ramizur Rahaman, He, Qian, Helyes, Zsuzsanna, Holm, Patrik, Huang, Huizhen, Hudhud, Lina, Iannone, Luigi Francesco, Islas, Leon D., Jaffal, Sahar Majdi, Jalava, Niina, Kashio, Makiko, Kiselyov, Kirill, Koivisto, Ari, Konkoly, János, Kormos, Viktória, Kovács-Rozmer, Katalin, Kriszta, Gábor, Kuhad, Anurag, Kumar, Shamit, Kumar, Vikash, Kumar, Satish, Kumar, Abhishek, Lal, Roshan, Leavell, Yaowaree, Lee, Jeewoo, Liang, Dong, Liu, Yusheng, Lopez, Kyle E., Lyu, Yihan, Mahapatra, Parnasree, McKemy, David D., Mishra, Subham, Mohanta, Sushama, Nadezhdin, Kirill D., Nassini, Romina, Oláh, Attila, Patel, Mihir, Pepelyayeva, Yuliya, Pertovaara, Antti, Phan, Thieu X., Pintér, Erika, Pires, Paulo W., Pohóczky, Krisztina, Porszász, Robert, Pozsgai, Gábor, Puri, Priyanka, Rangel-Yescas, Gisela E., Rantanen, Marja, Rardin, Benjamin, Rokade, Tejas Pravin, Rosenberg, Paul, Ross, Sarah E., Royal, Mike A., Sahu, Ram Prasad, Savu, Andrea, Shikha, Deep, Silva, Josiane F., Simpson, David, Sing, Raima, Snyder, Lindsey M., Sobolevsky, Alexander I., Sodhi, Rupinder Kaur, Stevens, Randall, Szallasi, Arpad, Szőke, Éva, Szöllősi, Attila Gábor, Tóth, Attila, Tóth, Balázs István, Van Horn, Wade D., Wang, Hongmei, Wortley, Michael A., Zhang, Jinwei, and Zhou, Zirui

Published
2024
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17. Somatostatin and Its Receptors in Myocardial Ischemia/Reperfusion Injury and Cardioprotection

Author

Vörös, Imre, primary, Sághy, Éva, additional, Pohóczky, Krisztina, additional, Makkos, András, additional, Onódi, Zsófia, additional, Brenner, Gábor B., additional, Baranyai, Tamás, additional, Ágg, Bence, additional, Váradi, Barnabás, additional, Kemény, Ágnes, additional, Leszek, Przemyslaw, additional, Görbe, Anikó, additional, Varga, Zoltán V., additional, Giricz, Zoltán, additional, Schulz, Rainer, additional, Helyes, Zsuzsanna, additional, and Ferdinandy, Péter, additional

Published
2021
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20. Characterization of Neurons Expressing the Novel Analgesic Drug Target Somatostatin Receptor 4 in Mouse and Human Brains

Author

Kecskés, Angéla, primary, Pohóczky, Krisztina, additional, Kecskés, Miklós, additional, Varga, Zoltán V., additional, Kormos, Viktória, additional, Szőke, Éva, additional, Henn-Mike, Nóra, additional, Fehér, Máté, additional, Kun, József, additional, Gyenesei, Attila, additional, Renner, Éva, additional, Palkovits, Miklós, additional, Ferdinandy, Péter, additional, Ábrahám, István M., additional, Gaszner, Balázs, additional, and Helyes, Zsuzsanna, additional

Published
2020
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22. Resolvin D1 and D2 Inhibit Transient Receptor Potential Vanilloid 1 and Ankyrin 1 Ion Channel Activation on Sensory Neurons via Lipid Raft Modification

Author

Payrits, Maja, primary, Horváth, Ádám, additional, Biró-Sütő, Tünde, additional, Erostyák, János, additional, Makkai, Géza, additional, Sághy, Éva, additional, Pohóczky, Krisztina, additional, Kecskés, Angéla, additional, Kecskés, Miklós, additional, Szolcsányi, János, additional, Helyes, Zsuzsanna, additional, and Szőke, Éva, additional

Published
2020
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24. Transient Receptor Potential Ankyrin 1 (TRPA1) and Vanilloid 1 (TRPV1) ion channels are expressed and upregulated in response to estrogen in the rat endometrium

Author

Pohóczky, Krisztina, primary, Kun, József, additional, Szalontai, Bálint, additional, Szőke, Éva, additional, Sághy, Éva, additional, Payrits, Maja, additional, Perkecz, Anikó, additional, Környei, József, additional, Garai, János, additional, Garami, András, additional, Kovács, Krisztina, additional, Kajtár, Béla, additional, Czeglédi, Levente, additional, and Helyes, Zsuzsanna, additional

Published
2017
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25. Local upregulation of transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1 ion channels in rectosigmoid deep infiltrating endometriosis

Author

Bohonyi, Noémi, primary, Pohóczky, Krisztina, additional, Szalontai, Bálint, additional, Perkecz, Anikó, additional, Kovács, Krisztina, additional, Kajtár, Béla, additional, Orbán, Lajos, additional, Varga, Tamás, additional, Szegedi, Sarolta, additional, Bódis, József, additional, Helyes, Zsuzsanna, additional, and Koppán, Miklós, additional

Published
2017
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27. Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Author

Pohóczky, Krisztina, primary, Kun, József, additional, Szalontai, Bálint, additional, Szőke, Éva, additional, Sághy, Éva, additional, Payrits, Maja, additional, Kajtár, Béla, additional, Kovács, Krisztina, additional, Környei, József László, additional, Garai, János, additional, Garami, András, additional, Perkecz, Anikó, additional, Czeglédi, Levente, additional, and Helyes, Zsuzsanna, additional

Published
2015
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30. Upregulation of the Transient Receptor Potential Ankyrin 1 Ion Channel in the Inflamed Human and Mouse Colon and Its Protective Roles

Author

Kun, József, primary, Szitter, István, additional, Kemény, Ágnes, additional, Perkecz, Anikó, additional, Kereskai, László, additional, Pohóczky, Krisztina, additional, Vincze, Áron, additional, Gódi, Szilárd, additional, Szabó, Imre, additional, Szolcsányi, János, additional, Pintér, Erika, additional, and Helyes, Zsuzsanna, additional

Published
2014
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31. Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium.

Author

Pohóczky, Krisztina, Kun, József, Szalontai, Bálint, Szöke, Éva, Sághy, Éva, Payrits, Maja, Kajtár, Béla, Kovács, Krisztina, Környei, József László, Garai, János, Garami, András, Perkecz, Anikó, Czeglédi, Levente, and Helyes, Zsuzsanna

Subjects
*STEROID hormones, *SEX hormones, *ORGANIC compounds, *PROTEINS, *LABORATORY mice
Abstract

Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors expressed predominantly in sensory nerves are activated by inflammatory stimuli and mediate inflammation and pain. Although they have been shown in the human endometrium, their regulation and function are unknown. Therefore, we investigated their estrogen- and progesterone-dependent alterations in the rat endometrium in comparison with the estrogen-regulated inflammatory cytokine macrophage migration inhibitory factor (MIF). Four-week-old (sexually immature) and four-month-old (sexually mature) female rats were treated with the non-selective estrogen receptor (ER) agonist diethylstilboestrol (DES), progesterone and their combination, or ovariectomized. RT-PCR and immunohistochemistry were performed to determine mRNA and protein expression levels respectively. Channel function was investigated with ratiometric [Ca2+]i measurement in cultured primary rat endometrial cells. Both TRP receptors and MIF were detected in the endometrium at mRNA and protein levels, and their localizations were similar. Immunostaining was observed in the immature epithelium, while stromal, glandular and epithelial positivity were observed in adults. Functionally active TRP receptor proteins were shown in endometrial cells by activation-induced calcium influx. In adults, Trpa1 and Trpv1 mRNA levels were significantly up-regulated after DES treatment. TRPA1 increased after every treatment, but TRPV1 remained unchanged following the combined treatment and ovariectomy. In immature rats, DES treatment resulted in increased mRNA expression of both channels and elevated TRPV1 immunopositivity. MIF expression changed in parallel with TRPA1/TRPV1 in most cases. DES up-regulated Trpa1, Trpv1 and Mif mRNA levels in endometrial cell cultures, but 17b-oestradiol having ERa-selective potency increased only the expression of Trpv1.We provide the first evidence for TRPA1/TRPV1 expression and their estrogen-induced up-regulation in the rat endometrium in correlation with the MIF. [ABSTRACT FROM AUTHOR]

Published
2016
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