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6 results on '"Pogorelov VI"'

1. [Eltor cholera in Latin America].

Author

Maramovich AS, Pogorelov VI, Urbanovich LIa, and Shkaruba TT

Subjects
Humans, Latin America epidemiology, Cholera epidemiology, Disease Outbreaks
Abstract

Cholera epidemics in South and Central America in the period 1991-1999 are characterized. The mechanisms of existence of Vibrio eltor in environmental objects have been substantiated. The causes and conditions of endemic foci formation which determine the infection spread not only within the countries of the subcontinent, but also far beyond its borders are shown. The effectiveness of cholera control interventions is evaluated.

Published
2001

2. [PCR-detection of markers for epidemiologically-significant strains of Vibrio cholerae 0139, isolated in Siberia].

Author

Balakhonov SV, Mironova LV, Maramovich AS, Ganin VS, Urbanovich LIa, and Pogorelov VI

Subjects
Base Sequence, Cholera microbiology, DNA Primers, Phenotype, Siberia epidemiology, Vibrio cholerae isolation & purification, Vibrio cholerae pathogenicity, Virulence genetics, Water Microbiology, Cholera epidemiology, Genetic Markers, Polymerase Chain Reaction methods, Vibrio cholerae genetics
Abstract

Fourteen V. cholerae 0139 strains were isolated in 1996-1999 in Siberia from the Ob river (Novosibirsk) and bogs and lakes (Irkutsk). The strains were tested in PCR for the key virulence determinants (ctx AB, tcp, acf). The genomes lacked these elements, and therefore the strains were acknowledged avirulent. The results correlate completely with the data of phenotypical analysis, characterizing the pathogenic characteristics of isolated strains.

Published
2001

3. [Comparative study of various methods for determining Vibrio cholerae toxigenicity].

Author

Balakhonov SV, Ganin VS, Urbanovich LIa, Pogorelov VI, and Golubinskiĭ EP

Subjects
DNA Probes, Enterotoxins toxicity, Hemolysis drug effects, Polymerase Chain Reaction, Vibrio cholerae genetics, Vibrio cholerae metabolism, Enterotoxins biosynthesis, Vibrio cholerae pathogenicity
Abstract

Testing of 138 Vibrio cholerae strains for gene determinants responsible for the production of cholera enterotoxin by the polymerase chain reaction (PCR) and gene probing using molecular CT-probe showed good correlation of the results of different methods and correlation of these data with studies of V. cholerae strain virulence in vivo and in hemolytic activity test. The advantages of PCR in rapid assessment of the toxigenicity and epidemic significance of V. cholerae strains are demonstrated.

Published
1999

4. [The interrelationships between Vibrio cholerae and the infusorian Tetrahymena pyriformis].

Author

Pogorelov VI, Pinigin AF, Maramovich AS, Pushkareva VI, Litvin VIu, Lykova MV, and Kapustin IuM

Subjects
Animals, Colony Count, Microbial, Microscopy, Electron, Tetrahymena pyriformis ultrastructure, Time Factors, Vibrio cholerae pathogenicity, Virulence, Water Microbiology, Tetrahymena pyriformis microbiology, Vibrio cholerae growth & development
Abstract

The results of the study of interaction between V. cholerae of different virulence and T. pyriformis are presented. The study has revealed the heterogeneity of V. cholerae population: alongside easily phagocytized vibrios, there are vibrios resistant to the digestive action of T. pyriformis. An increase in the number of V. cholerae in association with T. pyriformis has been evaluated, taking into account the selective multiplication of vibrios resistant to phagocytosis. The data on changes in the agglutinative, phagolytic and virulent properties of V. cholerae cultivated together with T. pyriformis are presented. The suggestion has been made that protozoa can function as hosts of pathogenic vibrios supporting their existence in water.

Published
1995

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