23 results on '"Plum Pox Virus (PPV)"'
Search Results
2. Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes
- Author
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Helena Skutkova, Dusan Pavlik, Petr Babula, Boris Krska, Rene Kizek, Ivo Provaznik, Ales Horna, Jiri Sochor, Vojtech Adam, and Ondrej Zitka
- Subjects
apricot ,DPPH ,TEAC ,FRAP ,high performance liquid chromatography (HPLC) ,electrochemical and spectrometric detection ,Plum pox virus (PPV) ,dendrogram ,Organic chemistry ,QD241-441 - Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH• (1,1-diphenyl-2-picryl-hydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (–)- and (+)- catechin.
- Published
- 2010
- Full Text
- View/download PDF
3. Preliminary results of in vivo thermotherapy of plum, apricot and peach cultivars artificially infected with PPV-M and PPV-D strains of Plum pox virus
- Author
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J. Polák and A. Hauptmanová
- Subjects
plum pox virus (ppv) ,apricot ,peach ,plum ,fruit trees ,thermotherapy in vivo ,Agriculture (General) ,S1-972 - Abstract
The elimination of Plum pox virus (PPV) in different stone fruit cultivars was verified by the method of thermotherapy in vivo. Trees of two plum cultivars Čačanská lepotica and Švestka domácí, apricot cultivars Leskora and Velkopavlovická, and peach cultivars Redhaven and Earliglo were used. They were infected artificially with two strains of the virus (PPV-D, PPV-M). Two cycles of thermotherapy in vivo were performed. During the first cycle, 16 trees of plum, apricot and peach were treated for 15 days at 37°C. In the second thermotherapy cycle, 10 trees of individual cultivars of plum, apricot and peach were treated for 22 days at 37°C. In the first thermotherapy (T1), 8 trees out of 16 died; PPV was eliminated in 2 trees of cv. Čačanská lepotica, 1 tree of cv. Švestka domácí and 2 trees of cv. Velkopavlovická. In the second thermotherapy (T2), 1 of 10 treated trees died. The virus was eliminated in 2 trees of cv. Čačanská lepotica, 1 tree of cv. Leskora, 2 trees of cv. Velkopavlovická, and 1 tree of cv. Redhaven. Nine (T1) and seven (T2) months after the thermotherapy, the presence of PPV was detected in 6 out of 11 originally recovered trees using ELISA. Out of 26 trees, 4 trees remained recovered: 2 plum trees and 2 apricot trees. One of these trees, apricot cv. Leskora was originally infected with PPV-M strain, whereas the other three with PPV-D strain. None of the 10 peach trees was treated successfully.
- Published
- 2009
- Full Text
- View/download PDF
4. Development of an on-site plum pox virus detection kit based on immunochromatography.
- Author
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Maejima, Kensaku, Himeno, Misako, Netsu, Osamu, Ishikawa, Kazuya, Yoshida, Tetsuya, Fujita, Naoko, Hashimoto, Masayoshi, Komatsu, Ken, Yamaji, Yasuyuki, and Namba, Shigetou
- Subjects
- *
VIRAL disease diagnosis , *STONE fruit diseases & pests , *JAPANESE apricot , *MESOPHYLL tissue , *ENZYME-linked immunosorbent assay - Abstract
Sharka disease, caused by plum pox virus (PPV), is the most serious viral disease of stone fruit trees. Among the eight known strains of the virus, PPV-D is the most important due to its recent global spread. Although enzyme-linked immunosorbent assay (ELISA) is the most common approach for diagnosing sharka, it involves time-consuming steps and requires expensive equipment and trained technicians. In this study, an on-site PPV detection kit based on immunochromatography was developed using polyclonal antibodies against the coat protein (CP) of a PPV-D isolate. The immunochromatographic (IC) assay kit was as sensitive as a commercial ELISA system for detecting Japanese PPV-D isolates. Moreover, it was easy to use (a one-step procedure), and results could be obtained on-site within 15 min without special laboratory equipment. The IC assay kit detected the virus from every aerial part of symptomatic Japanese apricot trees. In a detailed study of viral localization in leaves, the most suitable plant parts for use in the IC assay were symptomatic mesophyll tissues and the region from the petiole to the main vein. A positive reaction was also observed using the CP of other major (PPV-M and PPV-Rec) and minor (PPV-EA, PPV-W, and PPV-T) strains. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
5. Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes.
- Author
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Sochor, Jiri, Zitka, Ondrej, Skutkova, Helena, Pavlik, Dusan, Babula, Petr, Krska, Boris, Horna, Ales, Adam, Vojtech, Provaznik, Ivo, and Kizek, Rene
- Subjects
- *
HOLISTIC medicine , *PHENOLS , *APRICOT , *ANTIOXIDANTS , *POLYPHENOLS , *THERAPEUTICS - Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH• (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
6. Direct sample preparation methods for the detection of Plum pox virus by real-time RT-PCR.
- Author
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Capote, Nieves, Bertolini, Edson, Olmos, Antonio, Vidal, Eduardo, Martínez, M. Carmen, and Cambra, Mariano
- Subjects
- *
PLANT materials centers , *PLANT diseases , *NUCLEIC acids , *PRUNUS , *PLANT cells & tissues , *DORMANCY in plants - Abstract
Direct systems to process plant materials allowed high-throughput testing of Plum pox virus (PPV) by real-time reverse transcription (RT)-PCR without nucleic acids purification. Crude plant extracts were diluted in buffer or spotted on membranes to be used as templates. Alternatively, immobilized PPV targets were amplified from fresh sections of plant tissues printed or squashed onto the same supports, without extract preparation. Spot real-time RT-PCR was validated as a PPV diagnostic method in samples collected during the dormancy period and showed high sensitivity (93.6%), specificity (98.0%), and post-test probability (97.9%) towards sharka disease. In an analysis of 2919 Prunus samples by spot real-time RT-PCR and DASI-ELISA 90.8% of the results coincided, demonstrating high agreement (k = 0.77 ± 0.01) between the two techniques. These results validate the use of immobilized PPV targets and spot real-time RT-PCR as screening method for large-scale analyses. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
7. Transcript imaging and candidate gene strategy for the characterisation of Prunus/PPV interactions
- Author
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V. Decroocq, V. Schurdi-Levraud, D. Wawrzyńczak, J.P. Eyquard, and M. Lansac
- Subjects
plum pox virus (ppv) ,sharka disease ,prunus ,genetic resistance ,tolerance ,cdna-aflp ,candidate gene ,Plant culture ,SB1-1110 - Abstract
Plum pox virus (PPV), the causing agent of the sharka disease, belongs to the genus Potyvirus that contains the largest number of virus species infecting plants. The virus genome has been extensively characterised and sequenced. However, few data are available on its interactions with the host plant, Prunus. In this study, we are focusing on the cloning and characterisation of any candidate genes involved in the expression of the resistance/susceptibility trait and any polymorphic genes putatively involved in the trait variation. In order to clone candidate genes, two main approaches are currently developed: the homology cloning of genes presumed to affect the resistance/susceptibility trait and the differential screening of cDNA pools corresponding to infected and non-infected plant material. The second approach is based on the transcript imaging of the host plant response to PPV infection. Previously, it has been shown that infection by a potyvirus is associated with specific changes in host gene expression, mainly down-regulation, while the expression of some genes remained unchanged. Thereby, in the differential display approach combined to further characterisation of candidate gene expression, we aim to monitor host gene expression in response to the virus and to describe a highly regulated interaction between the Prunus host plant and the infecting Plum pox virus.
- Published
- 2002
- Full Text
- View/download PDF
8. Detection of plum pox virus infection in selection plum trees using spectral imaging
- Author
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Antoniy Stoev, Latchezar Avramov, Ekaterina Borisova, and L. Angelova
- Subjects
medicine.medical_specialty ,hуperspectral remote sensing (HRS) techniques ,light-induced fluorescence spectroscopy ,Spectral signature ,Diffuse reflectance infrared fourier transform ,food and beverages ,02 engineering and technology ,Biotic stress ,Biology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Reflectivity ,Spectral imaging ,diffuse reflectance spectroscopy ,010309 optics ,Horticulture ,plum pox virus (PPV) ,0103 physical sciences ,medicine ,Pox virus ,Cultivar ,0210 nano-technology ,Chlorophyll fluorescence - Abstract
Plum pox virus (PPV) is among the most studied viral diseases in the world in plants. It is considered to be one of the most devastating diseases of stone fruits in terms of agronomic impact and economic importance. Noninvasive, fast and reliable techniques are required for evaluation of the pathology in selection trees with economic impact. Such advanced tools for PPV detection could be optical techniques as light-induced fluorescence and diffuse reflectance spectroscopies. Specific regions in the electromagnetic spectra have been found to provide information about the physiological stress in plants, and consequently, diseased plants usually exhibit different spectral signature than non-stressed healthy plants in those specific ranges. In this study spectral reflectance and chlorophyll fluorescence were used for the identification of biotic stress caused by the pox virus on plum trees. The spectral responses of healthy and infected leaves from cultivars, which are widespread in Bulgaria were investigated. The two applied techniques revealed statistically significant differences between the spectral data of healthy plum leaves and those infected by PPV in the visible and near-infrared spectral ranges. Their application for biotic stress detection helps in monitoring diseases in plants using the different plant spectral properties in these spectral ranges. The strong relationship between the results indicates the applicability of diffuse reflectance and fluorescence techniques for conducting health condition assessments of vegetation and their importance for plant protection practices.
- Published
- 2017
9. First report of plum pox virus infecting Japanese apricot ( Prunus mume Sieb. et Zucc.) in Japan.
- Author
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Maejima, Kensaku, Hoshi, Hideo, Hashimoto, Masayoshi, Himeno, Misako, Kawanishi, Takeshi, Komatsu, Ken, Yamaji, Yasuyuki, Hamamoto, Hiroshi, and Namba, Shigetou
- Subjects
- *
JAPANESE apricot , *APRICOT , *TREE diseases & pests ,PLUM diseases & pests - Abstract
For the first time, plum pox virus (PPV) has been detected in commercial Japanese apricot ( Prunus mume) trees in Tokyo, Japan. These trees had ringspot or mottle on leaves, color breaking of petals and, occasionally, mild ringspots and malformation on fruits. The virus was identified based on the morphology of virus particles, serology, and RT-PCR. The amplified nucleotide fragment shared 100% identity with a partial coat protein gene of PPV-D isolates. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
10. AN AMINO ACID SUBSTITUTION OF THE COAT PROTEIN OF PLUM POX VIRUS ALTERS ITS MOBILITY ON SDS-PAGE
- Subjects
Coat protein ,modification ,Western-blot ,MALDI-TOF MS ,Plum pox virus (PPV) - Abstract
Plum pox virus (genus Potyvirus, family Potyviridae, PPV) is one of the most devastating plant viruses in the world. In 2009, PPV was first detected in Japanese apricot trees in Tokyo. We obtained four sample trees, Ak19-8, Ak19-9, Ak19-11, and Ak19-14, from a single field in Akiruno city, Tokyo. The symptoms of these trees were almost the same, however, by a Western-blot analysis, viral coat protein (CP) of Ak19-8 is apparently smaller than those of other three trees. We analyzed the sequences of their genomic RNAs and compared the deduced amino acid sequences of the CPs. We found difference in 66th amino acids, which was glycine in Ak19-8, while they were glutamic acid in Ak19-9, 11 and 14. This position is known to affect the manner of the modification of CP. It is also reported that CP was modified by O-linked N-acetylglucosamine (O-GlcNAc), which was suggested to work for host fitness. We separated the CPs of purified Ak19-8 and Ak19-14 particles by SDS-PAGE, and the CP bands were subjected to the MALDI-TOF MS analysis. The CP of Ak19-14 has two peaks correspond to the peptide of 40-93 amino acids carrying two and five O-GlcNAc, suggesting that the CP of the Japanese PPV are also modified by O-GlcNAc, and the manner of the modification could be different according to the 66th amino acid.
- Published
- 2015
11. Content of Phenolic Compounds and Antioxidant Capacity in Fruits of Apricot Genotypes
- Author
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Vojtech Adam, Aleš Horna, Ivo Provaznik, Petr Babula, Boris Krška, Dušan Pavlík, Jiri Sochor, Helena Skutkova, René Kizek, and Ondrej Zitka
- Subjects
0106 biological sciences ,DPPH ,Pharmaceutical Science ,electrochemical and spectrometric detection ,01 natural sciences ,Antioxidants ,Analytical Chemistry ,Ferulic acid ,chemistry.chemical_compound ,Flavonols ,Drug Discovery ,Caffeic acid ,ASSAY ,Food science ,Gallic acid ,2. Zero hunger ,chemistry.chemical_classification ,biology ,TEAC ,dendrogram ,ELECTROCHEMICAL DETECTION ,Agriculture ,Free Radical Scavengers ,04 agricultural and veterinary sciences ,040401 food science ,Prunus armeniaca ,CULTIVARS ,Chemistry (miscellaneous) ,high performance liquid chromatography (HPLC) ,Molecular Medicine ,Prunus ,LIQUID-CHROMATOGRAPHY ,VARIETIES ,Oxidation-Reduction ,Genotype ,Trolox equivalent antioxidant capacity ,Plum pox virus (PPV) ,Chemistry Techniques, Analytical ,Article ,lcsh:QD241-441 ,0404 agricultural biotechnology ,Phenols ,Chlorogenic acid ,lcsh:Organic chemistry ,Botany ,QUALITY ,Physical and Theoretical Chemistry ,apricot ,VEGETABLES ,Flavonoids ,FRAP ,Organic Chemistry ,Polyphenols ,FLAVONOIDS ,CONSUMPTION ,biology.organism_classification ,chemistry ,Fruit ,PLUM-POX-VIRUS ,010606 plant biology & botany - Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH center dot (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)-and (+)-catechin.
- Published
- 2010
12. ������������������ ������ �������������� ���������������������� ������������������ ������ ������ ������ ���������������� ������ ���������������������� (Plum pox virus, PPV) �������� ������������
- Subjects
������ ������������������������ ,Sharka ,�������������� ������ ���������������������� (PPV) ,Molecular and serological characterisation ,Plum pox virus (PPV) ,���������������� ������ �������������������� �������������������������� - Abstract
�� ������ ������ ���������������� ������ ���������������������� (Plum pox virus, PPV) ���������������� ���� ���������������� ���������� ������ ������������������������������������ ������������������ ������ ������������������������. �������� �������������� �������������� ���������� ������ ���������� �������� �������������������� ������ ���������������� �������������������������� ������ ���������������������� ������ ������ �������� ������������. �������������������� �������������������� ������ �������������������� ������ ���������������� �������������� �������������������� ������ ������ ���������������� ������ ���������� �������������������� ������ ����������������. �� ������ ����������������������, ���� ���������������� ����������������, ���� ������ ���� ���������������� ��������������������, �������������������� ������ ��������������������, ������ �������������������� ������ ���������������� ������ ��������������������. ���� ���������������� M ������������������ �������� ���������������� ��������, ������ �� ���������������� ������ ������������������ PPV-D �������������� ����������������, ���������� ������������������������������ �������� ���������� ���������������������� ������, ������ ���������������� ������ ������ �������� ��������������. ������ �������������� ������ ���������������� �������������� ������ ���������������������� ���������� ������������������ ������ ������������������������������ ���������������� Rec �� T. ���������������������� ������������ ������ �������������������� ���������������� NIb-CP ������ P3-6k1 ������������������ ������ ������������ ��������-������������������ �������������������������������� ������ ���������������������� PPV-M, ������ ���� ���������������������� PPV-D ���������� �������������������������� ������ �������������� �������������������� �������� ������������ ������ ������������ �������� ������������������ ��������. �������������������������� ������������������ ���������������� ������ ���������������� ������ CP ���������������������� M ������ ������������������������������ �������� �������������� ������������ ������������������ ������ ���������������������� ��������, �������� ������, ���� ���������������������� ������ ���� �������������������� ������������ (������������ Ma). ������ ������������������ �������������������������� ���� ���������� ������ ������ ���������������� ������ ������������������ ������������ (������������ Mb), ������ �� ��������������, ���������������� ���� ������������������ ��������������������, �������������������� ������������������ ������������, �������������� ���������� ������ ������������������������ ��������������. ����������, �������������� �������������� ���� ���������������������� �� ���������� ������ �������������������������� ���������������� ������ ������ �������������� P3-6k1, �������������� �������� ������ ������������������ �������������� ��������-�������������������� ���������������������������������� ������ ���������������������� PPV-M �������� �������������� ��������., Plum pox virus is the causal agent of the most devastating disease affecting Prunus sp. The present study is the first report of serological and molecular characterisation of Greek isolates. Comparative analysis of the sensitivity of the serological and molecular methods applied in this study proved the latter as the most reliable. The virus was detected via molecular testing in all apricot, peach, plum and P. maliformis samples. On the other hand, the virus was not detected in almond and cherry trees. The PPV-M strain is prevalent in Greek orchards, whereas only three PPV-D isolates were recovered, which were extremely geographically confined. No recombinant isolates, belonging to either the Rec or T strain, were detected in this study. Analysis of partial sequences of the NIb-CP and P3-6k1 polyprotein regions of PPV-M isolates revealed low inter-strain genetic variability. The PPV-D isolates identified herein were identical and were possibly introduced from another European country. Phylogenetic analysis of a fragment of the viral CP indicated that all but two of the PPV-M isolates recovered cluster with isolates hailing from the Mediterranean region (Ma clade). Of the remaining two, one clustered with isolates originating from Central and Eastern Europe (Mb clade) while the other shows an independent and presumably evolutionary intermediate route, between the two geographical groups. Finally, the phylogenetic hypothesis was not supported by a strong tree topology in the analysis of the P3-6k1 region, likely due to the high conservation of the aforementioned region amongst PPV-M isolates.
- Published
- 2015
- Full Text
- View/download PDF
13. Analogues of virus resistance genes map to QTLs for resistance to sharka disease in Prunus davidiana
- Author
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Decroocq, V., Foulongne, M., Lambert, P., Gall, O. Le., Mantin, C., Pascal, T., Schurdi-Levraud, V., and Kervella, J.
- Published
- 2005
- Full Text
- View/download PDF
14. Stability of gene silencing-based resistance to Plum pox virus in transgenic plum (Prunus domestica L.) under field conditions
- Author
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Hily, Jean-Michel, Scorza, Ralph, Malinowski, Tadeusz, Zawadzka, Barbara, and Ravelonandro, Michel
- Published
- 2004
- Full Text
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15. Optimization and implementation of a bimolecular fluorescence complementation (BiFC) system for the detection of plum pox potyviral protein-protein interactions in planta
- Author
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Zilian, Eva
- Subjects
bimolekulare Fluoreszenzkomplementation ,Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie ,bimolecular fluorescence complementation (BiFC) ,Protein-Protein Interaktionen ,ddc:570 ,protein-protein interactions ,Plum pox virus (PPV) - Abstract
[no abstract]
- Published
- 2011
- Full Text
- View/download PDF
16. Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus
- Author
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Sochor, Jiří, Zítka, Ondřej, Škutková, Helena, Pavlík, Dušan, Babula, Petr, Krška, Boris, Horna, Aleš, Adam, Vojtěch, Provazník, Ivo, and Kizek, René
- Subjects
high performance liquid chromatography (HPLC) ,TEAC ,dendrogram ,FRAP ,electrochemical and spectrometric detection ,apricot ,Plum pox virus (PPV) ,DPPH - Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.
- Published
- 2010
17. Direct sample preparation methods for the detection of Plum pox virus by real-time RT-PCR. Validation and practice parameters
- Author
-
Capote, Nieves, Bertolini, Edson, Olmos, Antonio, Vidal, Eduardo, Martínez, M. Carmen, Cambra, Mariano, Capote, Nieves, Bertolini, Edson, Olmos, Antonio, Vidal, Eduardo, Martínez, M. Carmen, and Cambra, Mariano
- Abstract
Direct systems to process plant materials allowed high-throughput testing of Plum pox virus (PPV) by real-time reverse transcription (RT)-PCR without nucleic acids purification. Crude plant extracts were diluted in buffer or spotted on membranes to be used as templates. Alternatively, immobilized PPV targets were amplified from fresh sections of plant tissues printed or squashed onto the same supports, without extract preparation. Spot real-time RT-PCR was validated as a PPV diagnostic method in samples collected during the dormancy period and showed high sensitivity (93.6%), specificity (98.0%), and post-test probability (97.9%) towards sharka disease. In an analysis of 2919 Prunus samples by spot real-time RT-PCR and DASI-ELISA 90.8% of the results coincided, demonstrating high agreement (k = 0.77 ± 0.01) between the two techniques. These results validate the use of immobilized PPV targets and spot real-time RT-PCR as screening method for largescale analyses. [Int Microbiol 2009; 12(1):1-6].
- Published
- 2010
18. Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus
- Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.
- Published
- 2010
19. Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus
- Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.
- Published
- 2010
20. Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus
- Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.
- Published
- 2010
21. Content of phenolic compounds and antioxidant capacity in fruits of selected genotypes of apricot with resistance against Plum pox virus
- Abstract
Research on natural compounds is increasingly focused on their effects on human health. In this study, we were interested in the evaluation of nutritional value expressed as content of total phenolic compounds and antioxidant capacity of new apricot (Prunus armeniaca L.) genotypes resistant against Plum pox virus (PPV) cultivated on Department of Fruit Growing of Mendel University in Brno. Fruits of twenty one apricot genotypes were collected at the onset of consumption ripeness. Antioxidant capacities of the genotypes were determined spectrometrically using DPPH (1,1-diphenyl-2-picrylhydrazyl free radicals) scavenging test, TEAC (Trolox Equivalent Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power)methods. The highest antioxidant capacities were determined in the genotypes LE-3228 and LE-2527, the lowest ones in the LE-985 and LE-994 genotypes. Moreover, close correlation (r = 0.964) was determined between the TEAC and DPPH assays. Based on the antioxidant capacity and total polyphenols content, a clump analysis dendrogram of the monitored apricot genotypes was constructed. In addition, we optimized high performance liquid chromatography coupled with tandem electrochemical and spectrometric detection and determined phenolic profile consisting of the following fifteen phenolic compounds: gallic acid, 4-aminobenzoic acid, chlorogenic acid, ferulic acid, caffeic acid, procatechin, salicylic acid, p-coumaric acid, the flavonols quercetin and quercitrin, the flavonol glycoside rutin, resveratrol, vanillin, and the isomers epicatechin, (-)- and (+)- catechin.
- Published
- 2010
22. Transformación genética del albaricoquero (Prunus armeniaca L.), mediada por Agrobacterium, y regeneración de plantas transformadas
- Author
-
Petri Serrano, César, Burgos Ortiz, Lorenzo, Departamentos y Servicios::Departamentos de la UMU::Biología Vegetal, and Universidad de Murcia. Departamento de Biología Vegetal
- Subjects
antibióticos aminoglicósidos ,Prunus armeniaca ,Regeneración de especies ,transformación ,gus ,genes marcadores ,Prunus armeniaca L ,Transformación genética ,progressive selection ,selección progresiva ,regeneración ,Albaricoqueros ,nptII ,Agrobacterium-tumefaciens ,marker genes ,Virus de la Sharka ,transformation ,aminoglycoside antibiotics ,gfp ,Plum Pox Virus (PPV) ,Biología Fundamental ,Agrobacterium tumefaciens ,regeneration - Abstract
Tesis doctoral, Departamento de Biología Vegetal de la Universidad de Murcia.-- Fecha de defensa: 23/07/2005. Calificación: Sobresaliente cum laude., En esta tesis se ha optimizado un protocolo de regeneración a partir de material varietal de ‘Helena’ y ‘Canino’ de albaricoquero (Prunus armeniaca L.). Mediante el estudio de los diversos factores que afectan la transformación de material adulto, se ha establecido por primera vez un protocolo eficiente de transformación mediada por Agrobacterium tumefaciens de una variedad comercial de albaricoquero. El diseño de una estrategia de selección gradual con paromomicina ha permitido la regeneración de plántulas transformadas con los genes marcadores nptII y sgfp o gus, con las eficiencias más elevadas que se han publicado hasta el momento para transformar material varietal en especies del género Prunus, aunque la baja viabilidad de las yemas transformadas redujo el número final de plantas obtenidas. El protocolo establecido en esta tesis sienta las bases que permitirán la introducción de genes de interés agronómico y comercial, modificando de manera discreta variedades élite aceptadas y establecidas en el mercado.
- Published
- 2005
23. Transformación genética del albaricoquero (Prunus armeniaca L.), mediada por Agrobacterium, y regeneración de plantas transformadas
- Author
-
Burgos Ortiz, Lorenzo, Petri Serrano, César, Burgos Ortiz, Lorenzo, and Petri Serrano, César
- Abstract
En esta tesis se ha optimizado un protocolo de regeneración a partir de material varietal de ‘Helena’ y ‘Canino’ de albaricoquero (Prunus armeniaca L.). Mediante el estudio de los diversos factores que afectan la transformación de material adulto, se ha establecido por primera vez un protocolo eficiente de transformación mediada por Agrobacterium tumefaciens de una variedad comercial de albaricoquero. El diseño de una estrategia de selección gradual con paromomicina ha permitido la regeneración de plántulas transformadas con los genes marcadores nptII y sgfp o gus, con las eficiencias más elevadas que se han publicado hasta el momento para transformar material varietal en especies del género Prunus, aunque la baja viabilidad de las yemas transformadas redujo el número final de plantas obtenidas. El protocolo establecido en esta tesis sienta las bases que permitirán la introducción de genes de interés agronómico y comercial, modificando de manera discreta variedades élite aceptadas y establecidas en el mercado.
- Published
- 2005
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