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210 results on '"Pliss, Artem"'

5. Laser-Ablative Engineering of ZrN-Based Nanoparticles for Photothermal Therapy and SERS-Based Biological Imaging.

Author

Pastukhov, Andrei I., Shipunova, Victoria O., Babkova, Julia S., Zelepukin, Ivan V., Raab, Micah, Schmitt, Rebecca, Al-Kattan, Ahmed, Pliss, Artem, Kuzmin, Andrey, Popov, Anton A., Klimentov, Sergey M., Prasad, Paras N., Deyev, Sergey M., and Kabashin, Andrei V.

Abstract

Zirconium nitride (ZrN) nanoparticles (NPs) can offer appealing plasmonic properties for biomedical applications, but the synthesis of nontoxic, water-dispersible nanoformulations exhibiting plasmonic features in the biotransparency window presents a great challenge. Here, we report the synthesis, by methods of laser ablation, of small ZrN-based NPs, which are unique in combining photothermal heating and near-field enhancement in the transparency window. Depending on the synthesis environment, the formed ZrN-based NPs exhibit plasmonic absorption bands with maxima around 660–670 and 610–630 nm, which are largely red-shifted compared to what is expected from pure ZrN NPs. The observed shift is explained by the inclusion of zirconium oxide ZrOx (1 < x < 2) into NP composition and NP coating by naturally formed ZrOx. We then explored biophotonic applications of ZrN NPs. While pure NPs demonstrate their nontoxicity in vitro, their conjugation with anti-HER1 affibody ZHER1:1907 and subsequent photothermal heating with NIR-I laser cause 100% cancer cell death. In addition, profiting from the field enhancement, we demonstrate bioimaging functionality using a designed surface-enhanced Raman scattering probe based on an NP-conjugated azobenzene-CN-OH molecule as a Raman reporter. Combining a strong photothermal effect and the imaging option, laser-synthesized ZrN/ZrOx NPs promise a major advancement of theranostic modalities based on plasmonic nanomaterials. [ABSTRACT FROM AUTHOR]

Published
2024
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8. IDH1 mutations induce organelle defects via dysregulated phospholipids

Author

Lita, Adrian, Pliss, Artem, Kuzmin, Andrey, Yamasaki, Tomohiro, Zhang, Lumin, Dowdy, Tyrone, Burks, Christina, de Val, Natalia, Celiku, Orieta, Ruiz-Rodado, Victor, Nicoli, Elena-Raluca, Kruhlak, Michael, Andresson, Thorkell, Das, Sudipto, Yang, Chunzhang, Schmitt, Rebecca, Herold-Mende, Christel, Gilbert, Mark R., Prasad, Paras N., and Larion, Mioara

Published
2021
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26. IDH1 Mutations Induce Organelle Defects Via Dysregulated Phospholipids

Author

Lita, Adrian, primary, Pliss, Artem, additional, Kuzmin, Andrey, additional, Yamasaki, Tomohiro, additional, Zhang, Lumin, additional, Dowdy, Tyrone, additional, Burks, Christina, additional, de Val, Natalia, additional, Celiku, Orieta, additional, Ruiz-Rodado, Victor, additional, Nicoli, Elena-Raluca, additional, Kruhlak, Michael, additional, Andresson, Thorkell, additional, Das, Sudipto, additional, Yang, Chunzhang, additional, Schmitt, Rebecca, additional, Herold-Mende, Christel, additional, Gilbert, Mark R., additional, Prasad, Paras N., additional, and Larion, Mioara, additional

Published
2020
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29. Toward Single-Organelle Lipidomics in Live Cells

Author

Lita, Adrian, primary, Kuzmin, Andrey N., additional, Pliss, Artem, additional, Baev, Alexander, additional, Rzhevskii, Alexander, additional, Gilbert, Mark R., additional, Larion, Mioara, additional, and Prasad, Paras N., additional

Published
2019
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47. Macromolecular Profiling of Organelles in Normal Diploid and Cancer Cells

Author

Levchenko, Svitlana M., Kuzmin, Andrey N., Pliss, Artem, Qu, Junle, and Prasad, Paras N.

Abstract

To advance an understanding of cellular regulation and function it is crucial to identify molecular contents in cellular organelles, which accommodate specific biochemical processes. Toward achievement of this goal, we applied micro-Raman-Biomolecular Component Analysis assay for molecular profiling of major organelles in live cells. We used this assay for comparative analysis of proteins 3D conformation and quantification of proteins, RNA, and lipids concentrations in nucleoli, endoplasmic reticulum, and mitochondria of WI 38 diploid lung fibroblasts and HeLa cancer cells. Obtained data show substantial differences in the concentrations and conformations of proteins in the studied organelles. Moreover, differences in the intraorganellar concentrations of RNA and lipids between these cell lines were found. We report the biological significance of obtained macromolecular profiles and advocate for micro-Raman BCA assay as a valuable proteomics tool.

Published
2024
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