106 results on '"Pliskova L"'
Search Results
2. Long-term protection against hepatitis B after newborn vaccination: 20-year follow-up
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Roznovsky, L., Orsagova, I., Kloudova, A., Tvrdik, J., Kabieszova, L., Lochman, I., Mrazek, J., Hozakova, L., Zjevikova, A., and Pliskova, L.
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- 2010
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3. Interleukin-6 measured using the automated electrochemiluminescence immunoassay method for the identification of intra-amniotic inflammation in preterm prelabor rupture of membranes
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Musilova I, Andrys C, Holeckova M, Kolarova V, Pliskova L, Drahosova M, Bolehovska R, Pilka R, Huml K, Cobo-Cobo T, Jacobsson B, and Kacerovsky M
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marker ,Amniotic fluid ,cytokine ,immunoassay method ,preterm birth - Abstract
Introduction: We aimed to compare the amniotic fluid interleukin (IL)-6 concentrations measured using the automated electrochemiluminescence immunoassay method and ELISA, and to establish an IL-6 concentration cut-off value for intra-amniotic inflammation (IAI) in preterm prelabor rupture of membranes (PPROM), which can be used in the automated electrochemiluminescence immunoassay method.Materials and methods: A total of 120 women with PPROM were included in this study. Amniotic fluid samples were obtained through transabdominal amniocentesis. IL-6 concentrations were assessed using both the automated electrochemiluminescence immunoassay method and ELISA, the current gold standard. IAI was defined as an amniotic fluid IL-6 concentration of =2600 pg/mL measured using ELISA.Results: A correlation between both assays was found (Spearman's rho = 0.97; p < .0001). Based on the receiver-operating characteristic curve for the identification of IAI (area under the curve = 0.99), a cut-off value of =3000 pg/mL was selected for the automated electrochemiluminescence immunoassay method with a sensitivity of 88%, specificity of 99%, positive predictive value of 97%, negative predictive value of 96%, and likelihood ratio of 76.Conclusions: For amniotic fluid IL-6 concentrations assessed using the automated electrochemiluminescence immunoassay method, a cut-off value of 3000 pg/mL was indicated for diagnosing IAI in women with PPROM.
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- 2020
4. Biochemical Prognostic Markers in Critically III Patients
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Zivny, P., Cerny, V., Zabka, L., Palicka, V., Pliskova, L., List, W. F., editor, Müller, M. M., editor, and McQueen, M. J., editor
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- 1997
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5. Clinical importance of PCR diagnosis in bacterial meningitis and sepsis
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Plisek, S., Pliskova, L., Dostal, V., Stepanova, V., Pozlerova, E., and Blazkova, J.
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- 2004
6. Vaccination against hepatitis B in newborns of HBsAg positive mothers
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Roznovsky, L., Orsagova, I., Lochman, I., Lukacova, L., Zjevikova, A., Sulakova, A., Pliskova, L., Kloudova, A., and Chovancik, J.
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- 2004
7. BAL fluid analysis in the identification of infectious agents in patients with hematological malignancies and pulmonary infiltrates
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Zak, P., primary, Vejrazkova, E., additional, Zavrelova, A., additional, Pliskova, L., additional, Ryskova, L., additional, Hubacek, P., additional, Stepanova, V., additional, Kostal, M., additional, Koblizek, V., additional, Paterova, P., additional, and Radocha, Jakub, additional
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- 2019
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8. Study of HCV seroprevalence in adult population in the Czech Republic
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Chlibek, R., primary, Stepanova, V., additional, Pliskova, L., additional, Smetana, J., additional, and Plisek, S., additional
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- 2016
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9. 13: Cervical microbiota in women with preterm pre labor rupture of membranes
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Kacerovsky, M., primary, Vrbacky, F., additional, Kutova, R., additional, Pliskova, L., additional, Andrys, C., additional, Musilova, I., additional, and Nekvindova, J., additional
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- 2015
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10. Sequence analysis (Sanger and NGS) in the detection of 2 mutations of CMV causing the resistance to ganciclovir – Comparison of two methods
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Pliskova, L., primary, Kutova, R., additional, Stepanova, V., additional, and Vejrazkova, E., additional
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- 2015
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11. CMV resistance in patients after allogeneic haematopoietic stem cell transplantation
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Hubacek, P., primary, Kouba, M., additional, Boutolleau, D., additional, Pliskova, L., additional, Vejrazkova, E., additional, Keslova, P., additional, Sedlacek, P., additional, and Cetkovsky, P., additional
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- 2015
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12. Evaluation of sustainable tourism potential of the principle Giant Mountains resorts in the Czech Republic
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Havlíková Michaela, Stupková Lucie Crespo, and Plíšková Lenka
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tourism potential ,sustainable tourism ,potential assessment ,the giant mountains ,Environmental sciences ,GE1-350 - Abstract
This paper proposes a new methodology for assessing the potential of sustainable tourism. It examines the overall potential of the landscape when faced with the negative impacts of tourism. Our assessment combines components of tourism and environmental sustainability. The methodology involved consultation with experts, and verification by tourists before being applied to the study area. The methodology was then applied to selected tourism centres in the Giant Mountains. The Giant Mountains are a popular tourist destination thanks to their outstanding natural beauty, and represent significant potential for tourism development. They are also one of the most over-burdened regions from tourism in the Czech Republic. However, many negative impacts of tourism exist, reducing the overall tourism potential of the region. Comparative results from the individual tourist centres in the study reveal the significant impact of potentially reducing attributes. Our assessment of the potential for sustainable tourism development in the area thus combines the environmental aspect of sustainable forms of tourism, with the identification of the most serious threats that need to be avoided to maintain the environment in the long-term. The results reveal the significant impact of excessive and inappropriate infrastructure and housing, as well as insufficient environmental education and legislation.
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- 2019
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13. OP13.10: An ultrasound transverse diameter of fetal thymus as a marker of the histological chorioamnionitis
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Kacerovsky, M., primary, Tosner, J., additional, Hornychova, H., additional, Pliskova, L., additional, and Forstl, M., additional
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- 2010
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14. O431 The correlation between sonographic transverse diameter of fetal thymus and intraamniotic infection in women with preterm prelabour rupture of membranes
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Kacerovsky, M., primary, Tosner, J., additional, Drahosova, M., additional, Andrys, C., additional, Pliskova, L., additional, and Hornychova, H., additional
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- 2009
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15. PIX-2 Parvovirus B19 infection in kidney transplant recipient
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Stepanova, V., primary, Pliskova, L., additional, Kubisova, M., additional, Zak, P., additional, Svobodova, M., additional, Plisek, S., additional, Stepanova, E., additional, Bolehovska, R., additional, and Dusilova-Sulkova, S., additional
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- 2009
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16. PX-17 Quality control and standardization processes in real-time PCR – the systems used in our laboratory
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Pliskova, L., primary, Bolehovska, R., additional, Stepanova, V., additional, and Plisek, S., additional
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- 2009
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17. Detection of parvovirus B19 infection by nested PCR assays
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Pliskova, L., primary, Bolehovska, R., additional, and Stepanova, V., additional
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- 2006
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18. Mumps outbreak in Eastern Bohemian region of Czech Republic
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Stepanova, V., primary, Pliskova, L., additional, Kosina, P., additional, Splino, M., additional, Forstl, M., additional, Plisek, S., additional, and Bolehovska, R., additional
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- 2006
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19. HBV DNA quantification by real-time PCR
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Plisek, S., primary, Pliskova, L., additional, Hrochova, K., additional, and Stepanova, V., additional
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- 2003
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20. The role of cytokines and antioxidant status in graft quality prediction
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Zivna, H, primary, Zivny, P, additional, Navratil, P, additional, Palicka, V, additional, Cerny, V, additional, Holeckova, M, additional, and Pliskova, L, additional
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- 1999
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21. Leptospirosis: possibilities of early laboratory and clinical diagnosis
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Cermakova Zuzana, Kucerova Petra, Valenta Zbynek, Pliskova Lenka, Bolehovska Radka, Prasil Petr, Buchta Vladimir, Scharfen Josef, Polak Pavel, Pavlis Ota, and Voxova Barbora
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leptospirosis ,early diagnosis ,pcr and mat ,clinical symptoms ,Medicine - Published
- 2013
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22. The significance of chronic Helicobacter pylori infection in critically ill patients | Vyznam chronicke infekce Helicobacter pylori u kriticky nemocnych
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Zivny, P., Cerny, V., Bures, J., Zivna, H., Vladimir Palicka, and Pliskova, L.
23. Dyserythropoietic changes in patients with hairy cell leukemia (HCL)
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Zak, P., Chrobak, L., Podzimek, K., Dulicek, P., Blaha, L., Pliskova, L., Voglova, J., Vladimir Maisnar, and Dedic, K.
24. Occurrence of CMV infection and disease in patients after non-myeloablative allogeneic hematopoietic stem cell transplantation (allo-NST)
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Zak, P., Kmonicek, M., Pliskova, L., Jebavy, L., Blaha, M., Jaroslav Malý, and Skorepova, M.
25. Serum soluble IL-2 receptor as a reliable and noninvasive marker of disease activity in patients with hairy cell leukemia
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Chrobak, L., Podzimek, K., Pliskova, L., Kerekes, Z., Zak, P., Voglova, J., Spacek, J., and Vladimir Palicka
26. The Use of Molecular Genetics Techniques in Clinical Microbiology - Final Report from the Workshop of the Molecular Microbiology Working Group TIDE
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Hrabak, J., Buncek, M., Dendis, M., Horvath, R., Chronakova, A., Libra, A., Nesvera, J., Pantucek, R., Piskunova, N., Pliskova, L., Ruzicka, F., Sauer, P., Sedlacek, I., Trubac, P., Zampachova, E., Helena Zemlickova, and Scharfen, J.
27. Ganciclovir treatment failure in adult allogeneic hematopoietic stem cell transplant recipients with cytomegalovirus infection - a single centre experience
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Vejrazkova, E., Hubacek, P., Kutova, R., Pliskova, L., Kostal, M., Stepanova, V., Zavrelova, A., Jakub Radocha, Mala, E., and Zak, P.
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Adult ,Male ,Incidence ,Hematopoietic Stem Cell Transplantation ,Cytomegalovirus ,Middle Aged ,Viral Load ,Antiviral Agents ,Postoperative Complications ,Cytomegalovirus Infections ,Drug Resistance, Viral ,Mutation ,Humans ,Transplantation, Homologous ,Female ,Treatment Failure ,Ganciclovir - Abstract
To determine the incidence of infection with ganciclovir-resistant cytomegalovirus (CMV) in adult allogeneic hematopoietic stem cell transplant (HSCT) recipients. Clinical resistance or treatment failure was defined as persistent DNAemia or increasing viral load in peripheral blood after 2 weeks of virostatic treatment. The association between the treatment failure and viral resistance was analysed. The presence of ganciclovir-resistant CMV strains was confirmed by genotypic testing able to detect mutations conferring resistance.In 2012 and 2014, 40 patients who underwent allogeneic HSCT for hematologic malignancies and were treated for human CMV reactivation/disease were followed up prospectively. In patients with treatment failure, CMV DNA was isolated and analysed by nucleotide sequence analysis of the UL 97 and UL 54 genes conferring resistance to the virostatic agent.The treatment failure occurred in seven patients, but ganciclovir resistance conferring mutations were only detected in two of them (mutations L595F and M460I in the UL 97 gene). Another mutation in the UL 97 gene (N510S) was found in a patient with recurrent CMV replication who needed to be retreated but did not meet the criteria for treatment failure.The low incidence of genetically confirmed ganciclovir-resistant CMV isolates in HSCT recipients with relatively common clinical treatment failure suggests that the mechanism underlying slower viral clearance is often other than mutations conferring ganciclovir resistance to the virus.
28. Brain Absceses Caused by Nocardia - Two Case Reports
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Plisek, S., Honegr, K., Kapla, J., Scharfen, J., Prasil, P., Kosina, P., Hobza, V., Talab, R., Elias, P., Ryska, P., Moravkova, M., Pliskova, L., Buncek, M., and Fridrichova, M.
29. The study of resistance of human cytomegalovirus to ganciclovir in patients after alogeneic transplantation of haematopoietic stem cells.
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Vlasta, Stepanova, Pliskova, L., Vejrazkova, E., Kutova, R., Hubacek, P., and Fajfr, M.
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DRUG resistance in microorganisms , *CYTOMEGALOVIRUS diseases , *GANCICLOVIR , *HEMATOPOIETIC stem cell transplantation , *PUBLIC health research - Published
- 2015
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30. The impact of the microbial load of genital mycoplasmas and gestational age on the intensity of intraamniotic inflammation.
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Kacerovsky M, Pliskova L, Bolehovska R, Skogstrand K, Hougaard DM, Tsiartas P, and Jacobsson B
- Abstract
OBJECTIVE: The objective of the study was to determine whether the bacterial load of genital mycoplasmas and gestation age are related to intraamniotic inflammatory response using the amniotic fluid levels of 18 inflammatory mediators. STUDY DESIGN: A prospective study of 145 women with singleton pregnancies complicated by preterm prelabor rupture of membranes between 24(0/7) and 36(6/7) weeks was conducted. Amniotic fluid was obtained from all women by transabdominal amniocentesis. The amounts of genital mycoplasma deoxyribonucleic acid were determined using the threshold cycle value and relative and absolute quantification techniques. A panel of multiple proteins was analyzed simultaneously using multiplex technology. RESULTS: Twenty-four women with the presence of genital mycoplasmas in amniotic fluid were included in the final analyses. The concentrations of 9 of the 18 evaluated proteins in the amniotic fluid correlated with bacterial load of genital mycoplasmas independent of the quantification technique used. CONCLUSION: The intensity of intraamniotic inflammatory response to genital mycoplasmas decreased with gestational age. [ABSTRACT FROM AUTHOR]
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- 2012
31. The microbial load with genital mycoplasmas correlates with the degree of histologic chorioamnionitis in preterm PROM.
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Kacerovsky M, Pliskova L, Bolehovska R, Musilova I, Hornychova H, Tambor V, and Jacobsson B
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MYCOPLASMA ,AMNIOTIC liquid ,BACTERIAL growth ,FETAL diseases ,LONGITUDINAL method ,MICROBIOLOGICAL techniques ,MYCOPLASMA diseases ,PREGNANCY complications ,PHYSIOLOGY - Abstract
OBJECTIVE: We sought to determine whether there is an association between bacterial load of genital mycoplasmas and histologic chorioamnionitis (HCA) in women with preterm premature rupture of membranes (PPROM). STUDY DESIGN: A total of 103 women with PPROM between 24-36 weeks of gestation were included in the study. Amniocenteses were performed, and the amounts of target genital mycoplasma DNA in amniotic fluid samples were evaluated using real-time polymerase chain reaction. The bacterial load of the genital mycoplasmas was relatively assessed using the threshold cycle value. RESULTS: The presence of genital mycoplasmas in amniotic fluid was found in 38% (39/103) of the women. The presence of HCA was associated with lower threshold cycle values (median 21.3, interquartile range, 16.5-28.5, vs median 29.4, interquartile range, 27.0-30.5; P = .005). CONCLUSION: HCA in PPROM is associated with a higher bacterial load of genital mycoplasmas. [ABSTRACT FROM AUTHOR]
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- 2011
32. Macrophage inflammatory protein-1α in amniotic and cervical fluids in spontaneous preterm labor with intact membranes with respect to intra-amniotic inflammation.
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Soucek O, Kacerovsky M, Stranik J, Musilova I, Pliskova L, Bolehovska R, Matulova J, and Andrys C
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- Pregnancy, Infant, Newborn, Female, Humans, Interleukin-6 metabolism, Chemokine CCL3 metabolism, Amniotic Fluid metabolism, Gestational Age, Inflammation metabolism, Chorioamnionitis microbiology, Obstetric Labor, Premature diagnosis, Obstetric Labor, Premature metabolism, Fetal Membranes, Premature Rupture metabolism
- Abstract
Objective: Macrophage inflammatory protein 1α is a chemokine produced by various immune, epithelial, mesothelial, and fibroblast cells after exposure to bacterial lipopolysaccharide or pro-inflammatory molecules. The primary aim of this study was to determine MIP-1α concentrations in amniotic and cervical fluids from pregnancy with spontaneous preterm labor with intact membranes (PTL) with respect to the presence of intra-amniotic infection (both microbial invasion of the amniotic cavity and intra-amniotic inflammation) and sterile intra-amniotic inflammation (intra-amniotic inflammation alone). The secondary aim was to assess the diagnostic indices of MIP-1α in predicting intra-amniotic infection., Materials and Methods: Seventy-four women with PTL were included in this study. Paired amniotic and cervical fluid samples were obtained using transabdominal amniocentesis and a Dacron polyester swab, respectively. Microbial invasion of the amniotic cavity was diagnosed based on a combination of culture and molecular biology methods. The concentration of IL-6 in the amniotic and cervical fluids was measured using an automated electrochemiluminescence immunoassay method. Intra-amniotic inflammation was defined as an amniotic fluid IL-6 concentration of ≥3000 pg/mL. The MIP-1α concentrations in the samples were assessed using an enzyme-linked immunosorbent assay., Results: A difference in amniotic fluid MIP-1α was observed among women with intra-amniotic infection, sterile intra-amniotic inflammation, and negative amniotic fluid (infection: median 1779.0 pg/mL; sterile, median 102.7 pg/mL; negative, median 19.9 pg/mL; p < .0001). No difference in the concentrations of MIP-1α was identified in cervical fluid after adjustment for gestational age at sampling (infection: median 77.7 pg/mL, sterile: median 152.7 pg/mL, negative: median 18.0 pg/mL; p = .30). The presence of intra-amniotic infection was associated with elevated MIP-1α concentrations in amniotic fluid (presence: 1779.0 pg/mL vs. absence: 26.3 pg/mL, p < .0001, area under receiver operating characteristic curve = 0.87)., Conclusions: In PTL pregnancies with the presence of intra-amniotic infection, the concentration of MIP-1α is elevated in amniotic fluid but not in cervical fluid. Amniotic fluid MIP-1α may provide a useful marker for intra-amniotic infection in women with PTL.
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- 2022
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33. Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes.
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Spacek R, Musilova I, Andrys C, Soucek O, Burckova H, Pavlicek J, Pliskova L, Bolehovska R, and Kacerovsky M
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- Amniotic Fluid, Female, Gestational Age, Granzymes, Humans, Infant, Newborn, Inflammation complications, Pregnancy, Chorioamnionitis diagnosis, Fetal Membranes, Premature Rupture etiology
- Abstract
Introduction: To determine the levels of granzyme A in amniotic fluid in pregnancies complicated by preterm prelabor rupture of membranes (PPROM), based on the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI)., Methods of Study: A total of 166 women with singleton pregnancies complicated by PPROM were included. Amniocentesis was performed at the time of admission and assessments of MIAC (using both cultivation and non-cultivation techniques) and IAI (interleukin-6 in amniotic fluid) were performed on all subjects. Based on the presence/absence of MIAC and IAI, the women were further divided into the following subgroups: intra-amniotic infection, sterile IAI, colonization, and absence of both MIAC and IAI. Amniotic fluid granzyme A levels were assessed using ELISA., Results: Women with MIAC had lower levels of granzyme A in the amniotic fluid than women without this condition (with MIAC: median 15.9 pg/mL vs. without MIAC: median 19.9 pg/mL, p = .03). Women with sterile IAI had higher amniotic fluid granzyme A levels than women with intra-amniotic infection, colonization and women with the absence of either MIAC or IAI (intra-amniotic infection: median 15.6 pg/mL; sterile IAI: median 31.8 pg/mL; colonization: median 16.9 pg/mL; absence of both MIAC and IAI: median 18.8 pg/mL; p = .02)., Conclusions: The presence of sterile IAI was associated with elevated levels of granzyme A in amniotic fluid.
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- 2022
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34. Amniotic fluid glucose level in PPROM pregnancies: a glance at the old friend.
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Kacerovsky M, Holeckova M, Stepan M, Gregor M, Vescicik P, Lesko D, Burckova H, Pliskova L, Bolehovska R, Andrys C, Jacobsson B, and Musilova I
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- Amniotic Fluid chemistry, Biomarkers analysis, Female, Friends, Gestational Age, Glucose, Humans, Infant, Infant, Newborn, Inflammation complications, Male, Pregnancy, Chorioamnionitis epidemiology, Chorioamnionitis etiology, Fetal Membranes, Premature Rupture etiology
- Abstract
Introduction: To determine the amniotic fluid glucose levels in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) based on the presence of microbial invasion of the amniotic cavity and/or intra-amniotic inflammation., Methods of Study: A total of 142 women with singleton pregnancies complicated by PPROM between gestational ages 24 + 0 and 36 + 6 weeks were included. Amniocentesis was performed at the time of admission. The assessments of microbial invasion of the amniotic cavity (using both cultivation and non-cultivation techniques) and intra-amniotic inflammation (amniotic fluid interleukin-6 levels ≥ 3000 pg/mL) were performed on all the women. Based on the presence of microbial invasion of the amniotic cavity and/or intra-amniotic inflammation, the women were further categorized into the subgroups: (i) intra-amniotic infection (the presence of both microbial invasion of the amniotic cavity and intra-amniotic inflammation); (ii) sterile intra-amniotic inflammation (the presence of intra-amniotic inflammation without microbial invasion of the amniotic cavity); (iii) colonization (the presence of microbial invasion of the amniotic cavity without intra-amniotic inflammation); and (iv) negative amniotic fluid (the absence of either microbial invasion of the amniotic cavity or intra-amniotic inflammation). Amniotic fluid glucose levels were assessed using enzymatic reference method with hexokinase., Results: There was a difference in the amniotic fluid glucose levels among the women with intra-amniotic infection, sterile intra-amniotic inflammation, colonization, and those with negative amniotic fluid ( p < .0001). No difference was found in the amniotic fluid glucose levels between women with intra-amniotic infection and those with sterile intra-amniotic inflammation [infection: median 11.6 mg/dL (0.7 mmol/L) vs. sterile: median 6.3 mg/dL (0.4 mmol/L); p = .41] and between women with colonization and negative amniotic fluid [colonization: median 21.6 mg/dL (1.2 mmol/L) vs. negative: median 23.4 mg/dL (1.3 mmol/L; p = .67]. Women with intra-amniotic infection and sterile intra-amniotic inflammation had lower amniotic fluid glucose levels than women with colonization and with negative amniotic fluid in crude analysis as well as after adjustment for gestational age at sampling. Amniotic fluid glucose level of 10 mg/dL (0.56 mmol/L) was the optimal concentration for the identification of intra-amniotic inflammation in women with PPROM., Conclusions: The presence of intra-amniotic inflammation was associated with lower amniotic fluid glucose levels in singleton pregnancies complicated with PPROM. An amniotic fluid glucose level of 10 mg/dL (0.56 mmol/L) was the optimal concentration for the identification of intra-amniotic inflammation in PPROM pregnancies. In the absence of better amniotic fluid markers, amniotic glucose could be used as a marker of intra-amniotic inflammation, with very good specificity in PPROM pregnancies.
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- 2022
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35. Amniotic fluid CD11b levels in pregnancies complicated by preterm prelabor rupture of membranes.
- Author
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Soucek O, Kacerovsky M, Musilova I, Pliskova L, Bolehovska R, and Andrys C
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- Amniocentesis, Amniotic Fluid metabolism, Female, Gestational Age, Humans, Infant, Newborn, Pregnancy, Chorioamnionitis metabolism, Fetal Membranes, Premature Rupture metabolism
- Abstract
Objective: CD11b is an integrin molecule located on the surface of leukocytes. CD11b is involved in the processes of cell adhesion and migration. Expression of CD11b increases during inflammation. Therefore, this study was aimed at the evaluation of concentrations of CD11b in the amniotic fluid from pregnancies complicated by preterm prelabor rupture of the membranes (PPROM), with respect to the presence of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and microbial-associated IAI (the presence of both MIAC and IAI)., Methods: Eighty women with singleton pregnancies complicated by PPROM were included. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid CD11b concentrations were determined by enzyme-linked immunosorbent assay. MIAC was determined by a non-cultivation approach. IAI was defined by a bedside amniotic fluid interleukin-6 concentration ≥745 pg/mL., Result: Women with MIAC or microbial-associated IAI had higher CD11b concentrations in the amniotic fluid than women without these complications (with MIAC: median 0.31 ng/mL versus without MIAC: median 0.17 ng/mL, p = .001; with microbial associated-IAI: median 0.35 ng/mL versus without microbial-associated IAI: median 0.16 ng/mL; p =.02). The presence of IAI was not associated with elevated CD11b concentrations. A weak negative correlation was found between amniotic fluid CD11b concentrations and interleukin-6 concentrations (rho = 0.26; p = .02)., Conclusions: MIAC and microbial-associated IAI are characterized by higher amniotic fluid CD11b concentrations in pregnancies complicated by PPROM.
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- 2022
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36. Presence of Chlamydia trachomatis DNA in the amniotic fluid in women with preterm prelabor rupture of membranes.
- Author
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Kacerovsky M, Romero R, Pliskova L, Bolehovska R, Hornychova H, Matejkova A, Vosmikova H, Andrys C, Kolackova M, Laudański P, Pelantova V, Jacobsson B, and Musilova I
- Subjects
- Amniotic Fluid, Chlamydia trachomatis, DNA, Female, Humans, Infant, Newborn, Interleukin-6, Pregnancy, Retrospective Studies, Chorioamnionitis epidemiology, Fetal Membranes, Premature Rupture
- Abstract
Objective: The primary aim of this study was to assess the rate and load of amniotic fluid Chlamydia trachomatis DNA and their associations with intra-amniotic infection and intra-uterine inflammatory complications in women with preterm prelabor rupture of membranes (PPROM). The secondary aim was to assess the short-term morbidity of newborns from PPROM pregnancies complicated by amniotic fluid C. trachomatis DNA., Methods: A retrospective study of 788 women with singleton pregnancies complicated by PPROM between 24 + 0 and 36 + 6 weeks of gestation was performed. Transabdominal amniocenteses were performed at the time of admission. C. trachomatis DNA in the amniotic fluid was assessed by real-time polymerase chain reaction using a commercial AmpliSens® C. trachomatis/Ureaplasma / Mycoplasma hominis -FRT kit, and the level of Ct DNA was quantified., Results: Amniotic fluid C. trachomatis DNA complicated 2% (16/788) of the PPROM pregnancies and was present in very low loads (median 57 copies DNA/mL). In addition to amniotic fluid C. trachomatis DNA, other bacteria were detected in 62% (10/16) of the C. trachomatis DNA-complicated PPROM pregnancies. Amniotic fluid C. trachomatis DNA was associated with intra-amniotic infection, histologic chorioamnionitis (HCA), and funisitis in 31%, 47%, and 33%, respectively. The presence of C. trachomatis DNA accompanied by Ureaplasma species in the amniotic fluid was associated with a higher rate of HCA than the presence of amniotic fluid C. trachomatis DNA alone. The composite neonatal morbidity in newborns from PPROM pregnancies with amniotic fluid C. trachomatis DNA was 31%., Conclusion: The presence of C. trachomatis DNA in the amniotic fluid is a relatively rare condition in PPROM. Amniotic fluid C. trachomatis DNA in PPROM is not related to intensive intra-amniotic and intr-auterine inflammatory responses or adverse short-term neonatal outcomes.
- Published
- 2021
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37. IgGFc-binding protein in pregnancies complicated by spontaneous preterm delivery: a retrospective cohort study.
- Author
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Stranik J, Kacerovsky M, Soucek O, Kolackova M, Musilova I, Pliskova L, Bolehovska R, Bostik P, Matulova J, Jacobsson B, and Andrys C
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- Adult, Biomarkers metabolism, Female, Humans, Pregnancy, Retrospective Studies, Amniotic Fluid metabolism, Cell Adhesion Molecules metabolism, Pregnancy Complications, Infectious metabolism, Premature Birth metabolism
- Abstract
To determine the IgGFc-binding protein (FcgammaBP) concentration in amniotic and cervical fluids in preterm prelabor rupture of membranes (PPROM) and preterm labor with intact membranes (PTL) and to assess the diagnostic indices of FcgammaBP to predict intra-amniotic infection (the presence of both microbial invasion of the amniotic cavity and intra-amniotic inflammation). In this study, we included 170 and 79 women with PPROM and PTL, respectively. Paired cervical and amniotic fluid samples were obtained using a Dacron polyester swab and transabdominal amniocentesis, respectively. The FcgammaBP concentrations in the samples were assessed using an enzyme-linked immunosorbent assay. The presence of intra-amniotic infection was associated with elevated FcgammaBP concentrations in pregnancies with PPROM and PTL [PPROM-presence: 86 ng/mL vs. absence: 13 ng/mL, p < 0.0001, area under receiver operating characteristic curve (AUC) = 0.94; PTL-presence: 140 ng/mL vs. absence: 22 ng/mL, p < 0.0001, AUC = 0.86]. In cervical fluid, the concentrations of FcgammaBP were elevated in the presence of intra-amniotic infection in pregnancies with PPROM only (presence: 345 ng/mL vs. absence: 60 ng/mL, p < 0.0001, AUC = 0.93). FcgammaBP in amniotic fluid might be a marker of intra-amniotic infection in women with both PPROM and PTL However, in cervical fluid, it is only observed in women with PPROM.
- Published
- 2021
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38. Cervical Gardnerella vaginalis in women with preterm prelabor rupture of membranes.
- Author
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Kacerovsky M, Pliskova L, Bolehovska R, Lesko D, Gerychova R, Janku P, Matlak P, Simetka O, Stranik J, Faist T, Mls J, Vescicik P, Jacobsson B, and Musilova I
- Subjects
- Adult, Amniotic Fluid chemistry, Chorioamnionitis microbiology, Female, Humans, Interleukin-6 analysis, Pregnancy, Prospective Studies, Amniotic Fluid microbiology, Cervix Uteri microbiology, Fetal Membranes, Premature Rupture microbiology, Gardnerella vaginalis isolation & purification
- Abstract
Objective: To determine the association between microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) and the cervical prevalence of Gardnerella vaginalis DNA in pregnancies with preterm prelabor rupture of membrane (PPROM)., Method: In total, 405 women with singleton pregnancies complicated with PPROM were included. Cervical fluid and amniotic fluid samples were collected at the time of admission. Bacterial and G. vaginalis DNA were assessed in the cervical fluid samples using quantitative PCR technique. Concentrations of interleukin-6 and MIAC were evaluated in the amniotic fluid samples. Loads of G. vaginalis DNA ≥ 1% of the total cervical bacterial DNA were used to define the cervical prevalence of G. vaginalis as abundant. Based on the MIAC and IAI, women were categorized into four groups: with intra-amniotic infection (both MIAC and IAI), with sterile IAI (IAI without MIAC), with MIAC without IAI, and without either MIAC or IAI., Results: The presence of the abundant cervical G. vaginalis was related to MIAC (with: 65% vs. without: 44%; p = 0.0004) but not IAI (with: 52% vs. without: 48%; p = 0.70). Women with MIAC without IAI had the highest load of the cervical G. vaginalis DNA (median 2.0 × 104 copies DNA/mL) and the highest presence of abundant cervical G. vaginalis (73%)., Conclusions: In women with PPROM, the presence of cervical G. vaginalis was associated with MIAC, mainly without the concurrent presence of IAI., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2021
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39. Antibiotic administration reduces the rate of intraamniotic inflammation in preterm prelabor rupture of the membranes.
- Author
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Kacerovsky M, Romero R, Stepan M, Stranik J, Maly J, Pliskova L, Bolehovska R, Palicka V, Zemlickova H, Hornychova H, Spacek J, Jacobsson B, Pacora P, and Musilova I
- Subjects
- Adult, Amniotic Fluid chemistry, Bacterial Infections etiology, Chorioamnionitis etiology, Cohort Studies, DNA, Bacterial analysis, Female, Humans, Interleukin-6 analysis, Pregnancy, Retrospective Studies, Ureaplasma genetics, Anti-Bacterial Agents therapeutic use, Bacterial Infections prevention & control, Chorioamnionitis prevention & control, Clarithromycin therapeutic use, Clindamycin therapeutic use, Fetal Membranes, Premature Rupture, Penicillin G therapeutic use
- Abstract
Background: Preterm prelabor rupture of the membranes (PPROM) is frequently complicated by intraamniotic inflammatory processes such as intraamniotic infection and sterile intraamniotic inflammation. Antibiotic therapy is recommended to patients with PPROM to prolong the interval between this complication and delivery (latency period), reduce the risk of clinical chorioamnionitis, and improve neonatal outcome. However, there is a lack of information regarding whether the administration of antibiotics can reduce the intensity of the intraamniotic inflammatory response or eradicate microorganisms in patients with PPROM., Objective: The first aim of the study was to determine whether antimicrobial agents can reduce the magnitude of the intraamniotic inflammatory response in patients with PPROM by assessing the concentrations of interleukin-6 in amniotic fluid before and after antibiotic treatment. The second aim was to determine whether treatment with intravenous clarithromycin changes the microbial load of Ureaplasma spp DNA in amniotic fluid., Study Design: A retrospective cohort study included patients who had (1) a singleton gestation, (2) PPROM between 24+0 and 33+6 weeks, (3) a transabdominal amniocentesis at the time of admission, and (4) intravenous antibiotic treatment (clarithromycin for patients with intraamniotic inflammation and benzylpenicillin/clindamycin in the cases of allergy in patients without intraamniotic inflammation) for 7 days. Follow-up amniocenteses (7
th day after admission) were performed in the subset of patients with a latency period lasting longer than 7 days. Concentrations of interleukin-6 were measured in the samples of amniotic fluid with a bedside test, and the presence of microbial invasion of the amniotic cavity was assessed with culture and molecular microbiological methods. Intraamniotic inflammation was defined as a bedside interleukin-6 concentration ≥745 pg/mL in the samples of amniotic fluid. Intraamniotic infection was defined as the presence of both microbial invasion of the amniotic cavity and intraamniotic inflammation; sterile intraamniotic inflammation was defined as the presence of intraamniotic inflammation without microbial invasion of the amniotic cavity., Results: A total of 270 patients with PPROM were included in this study: 207 patients delivered within 7 days and 63 patients delivered after 7 days of admission. Of the 63 patients who delivered after 7 days following the initial amniocentesis, 40 underwent a follow-up amniocentesis. Patients with intraamniotic infection (n = 7) and sterile intraamniotic inflammation (n = 7) were treated with intravenous clarithromycin. Patients without either microbial invasion of the amniotic cavity or intraamniotic inflammation (n = 26) were treated with benzylpenicillin or clindamycin. Treatment with clarithromycin decreased the interleukin-6 concentration in amniotic fluid at the follow-up amniocentesis compared to the initial amniocentesis in patients with intraamniotic infection (follow-up: median, 295 pg/mL, interquartile range [IQR], 72-673 vs initial: median, 2973 pg/mL, IQR, 1750-6296; P = .02) and in those with sterile intraamniotic inflammation (follow-up: median, 221 pg/mL, IQR 118-366 pg/mL vs initial: median, 1446 pg/mL, IQR, 1300-2941; P = .02). Samples of amniotic fluid with Ureaplasma spp DNA had a lower microbial load at the time of follow-up amniocentesis compared to the initial amniocentesis (follow-up: median, 1.8 × 104 copies DNA/mL, 2.9 × 104 to 6.7 × 108 vs initial: median, 4.7 × 107 copies DNA/mL, interquartile range, 2.9 × 103 to 3.6 × 107 ; P = .03)., Conclusion: Intravenous therapy with clarithromycin was associated with a reduction in the intensity of the intraamniotic inflammatory response in patients with PPROM with either intraamniotic infection or sterile intraamniotic inflammation. Moreover, treatment with clarithromycin was related to a reduction in the load of Ureaplasma spp DNA in the amniotic fluid of patients with PPROM <34 weeks of gestation., (Copyright © 2020 Elsevier Inc. All rights reserved.)- Published
- 2020
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40. Lactobacilli-dominated cervical microbiota in women with preterm prelabor rupture of membranes.
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Kacerovsky M, Pliskova L, Bolehovska R, Gerychova R, Janku P, Matlak P, Simetka O, Faist T, Mls J, Vescicik P, Zemlickova H, Jacobsson B, and Musilova I
- Subjects
- Cervix Uteri microbiology, Chlamydia trachomatis, Female, Humans, Infant, Newborn, Microbiota, Mycoplasma hominis, Obstetric Labor, Premature, Pregnancy, Retrospective Studies, Ureaplasma, Amniocentesis methods, Amniotic Fluid microbiology, Chorioamnionitis microbiology, Fetal Membranes, Premature Rupture microbiology, Lactobacillus, Lactobacillus crispatus
- Abstract
Background: To determine the association between microbial invasion of the amniotic cavity (MIAC) and the presence of Lactobacillus crispatus- or Lactobacillus iners-dominated cervical microbiota in pregnancies with preterm prelabor rupture of membrane. Next, to assess the relationship between the presence of L. crispatus- or L. iners-dominated cervical microbiota and short-term neonatal morbidity., Method: A total of 311 women were included. Cervical samples were obtained using a Dacron polyester swab and amniotic fluid samples were obtained by transabdominal amniocentesis. Bacterial DNA, L. crispatus, and L. iners in the cervical samples were assessed by PCR. Cervical microbiota was assigned as L. crispatus- or L. iners-dominated when the relative abundance of L. crispatus or L. iners was ≥50% of the whole cervical microbiota, respectively., Results: Women with MIAC showed a lower rate of L. crispatus-dominated cervical microbiota (21% vs. 39%; p = 0.003) than those without MIAC. Lactobacillus crispatus-dominated cervical microbiota was associated with a lower rate of early-onset sepsis (0% vs. 5%; p = 0.02)., Conclusions: The presence of L. crispatus-dominated cervical microbiota in women with preterm prelabor rupture of membrane was associated with a lower risk of intra-amniotic complications and subsequent development of early-onset sepsis of newborns.
- Published
- 2020
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41. Improved laboratory diagnostics of Streptococcus pneumoniae in respiratory tract samples through qPCR.
- Author
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Kukla R, Bolehovska R, Radocha J, Pliskova L, Zak P, Vrbacky F, Nekvindova J, and Zemlickova H
- Subjects
- DNA, Bacterial genetics, Humans, Respiratory System microbiology, Sensitivity and Specificity, Streptococcus pneumoniae genetics, Community-Acquired Infections diagnosis, Community-Acquired Infections microbiology, Pneumonia, Pneumococcal diagnosis, Pneumonia, Pneumococcal microbiology, Real-Time Polymerase Chain Reaction
- Abstract
The aim of this study was to test the detection performance of the cpsA, lytA and ply genes through qPCR in the identification of Streptococcus pneumoniae in respiratory tract samples. Specificity was tested on a panel of 128 streptococci and other bacteria DNA samples. The qPCR assay was tested on a total of 51 respiratory tract samples from patients with community-acquired pneumonia (CAP). The specificity of the cpsA, lytA and ply genes was 100%, 100%, and 86%, respectively. The quantitative assessment, based on lytA, determined a cutoff value of ~2x104, 4x102 and 4x102 DNA copies per 1 mL of valid sputum, tracheal aspirate and bronchial aspirate samples, respectively. The results from the present study suggest that qPCR detection of all three genes would be optimal in the accurate detection of Streptococcus pneumoniae.
- Published
- 2020
42. Herpes simplex virus resistant to acyclovir: A single-centre experience from the Czech Republic.
- Author
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Fajfr M, Pliskova L, Bolehovská R, Uhlířová Z, and Vrbacký F
- Subjects
- Czech Republic, DNA-Directed DNA Polymerase genetics, Exodeoxyribonucleases genetics, Herpes Simplex drug therapy, Herpes Simplex virology, Humans, Microbial Sensitivity Tests, Mutation, Retrospective Studies, Treatment Failure, Viral Proteins genetics, Acyclovir pharmacology, Antiviral Agents pharmacology, Drug Resistance, Viral genetics, Simplexvirus drug effects, Simplexvirus genetics
- Abstract
Introduction and Aim: Infections caused by herpes simplex viruses (HSV) are frequent in the human population. Because of the widespread use of long-term treatment or prophylaxis by anti-herpetic antivirals in various specific medical contexts (immunosuppression, recurrent infections), the level of antiviral resistance is increasing. According to previous studies, there is a low resistance level in immunocompetent populations but a relatively high level in populations with immunodeficiency. However, there has been no study from the Czech Republic. This study presents results of a single-centre retrospective study from the Czech Republic., Materials and Methods: Deep frozen DNA from patients with suspected clinical antiviral failure over a long time period (2009-2016) - a total of 15 isolates of HSV1 and seven of HSV2 - were examined for the presence of mutations associated with antiviral resistance. Sequence analysis was performed using an ABI PRISM 3500xL Genetic Analyzer (Applied Biosystems®)., Results: There were no mutations associated with resistance to antivirals inside the UL23 gene in HSV1 isolates. However, resistant mutation D672N (nucleotide change G2014A) was found inside the UL30 gene in seven of the isolates. One mutation associated with resistance to acyclovir (M183stop) was found inside the UL23 gene in one HSV2 isolate. Resistant mutation E678G (nucleotide change A2033G) was identified inside the UL30 gene in six of the HSV2 isolates., Conclusions: This study confirmed the presence of resistance mutations within the Czech population, but it will be necessary to examine a higher number of isolates for further conclusions., (Copyright © 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2019
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43. Clinical and genotypic CMV drug resistance in HSCT recipients: a single center epidemiological and clinical data.
- Author
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Vejrazkova E, Pliskova L, Hubacek P, Kostal M, Zavrelova A, Radocha J, Kutova R, Stepanova V, and Zak P
- Subjects
- Adult, Aged, Allografts, DNA, Viral genetics, Female, Follow-Up Studies, Humans, Male, Middle Aged, Prospective Studies, Cytomegalovirus genetics, Cytomegalovirus Infections drug therapy, Cytomegalovirus Infections genetics, Cytomegalovirus Infections mortality, Drug Resistance, Viral genetics, Hematologic Diseases genetics, Hematologic Diseases mortality, Hematologic Diseases therapy, Hematologic Diseases virology, Hematopoietic Stem Cell Transplantation, Transplantation Conditioning, Valganciclovir administration & dosage
- Published
- 2019
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44. Pentraxin 3 in Noninvasively Obtained Cervical Fluid Samples from Pregnancies Complicated by Preterm Prelabor Rupture of Membranes.
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Janku P, Kacerovsky M, Zednikova B, Andrys C, Kolackova M, Drahosova M, Pliskova L, Zemlickova H, Gerychova R, Simetka O, Matlak P, Jacobsson B, and Musilova I
- Subjects
- Amniotic Fluid microbiology, Biomarkers metabolism, Chorioamnionitis diagnosis, Chorioamnionitis microbiology, Enzyme-Linked Immunosorbent Assay, Female, Fetal Membranes, Premature Rupture diagnosis, Fetal Membranes, Premature Rupture microbiology, Humans, Predictive Value of Tests, Pregnancy, Retrospective Studies, Up-Regulation, C-Reactive Protein metabolism, Cervix Uteri metabolism, Chorioamnionitis metabolism, Fetal Membranes, Premature Rupture metabolism, Serum Amyloid P-Component metabolism
- Abstract
Problem: To determine the changes of pentraxin 3 (PTX3) level in noninvasively obtained cervical fluid samples from women with preterm prelabor rupture of membranes (PPROM) based on the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI), and intra-amniotic infection (the presence of both MIAC and IAI)., Methods of Study: A total of 160 women with PPROM were included. Cervical fluid samples were obtained using a Dacron polyester swab and amniotic fluid samples were obtained by transabdominal amniocentesis. Cervical fluid PTX3 levels were assessed using enzyme-linked immunosorbent assay., Results: PTX3 was found in all the cervical fluid samples and its levels were higher in women with MIAC, IAI, and intra-amniotic infection than in women without these conditions. When the women were categorized into four subgroups based on the presence of MIAC and/or IAI, women with intra-amniotic infection had higher cervical fluid PTX3 levels than those with sterile IAI (IAI alone), colonization (MIAC alone), or no MIAC or IAI. A cervical fluid PTX3 level of 11 ng/mL was the best value for identifying the presence of intra-amniotic infection in women with PPROM., Conclusions: PTX3 is a constituent of cervical fluid of women with PPROM. Cervical fluid PTX3 level reflects the situation in the intra-amniotic compartments of women with PPROM. Cervical fluid PTX3 is a potential marker for the noninvasive identification of intra-amniotic infection in PPROM., (© 2019 S. Karger AG, Basel.)
- Published
- 2019
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45. Amniotic fluid cell-free DNA in preterm prelabor rupture of membranes.
- Author
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Kacerovsky M, Vlkova B, Musilova I, Andrys C, Pliskova L, Zemlickova H, Stranik J, Halada P, Jacobsson B, and Celec P
- Subjects
- Adult, Amniocentesis, Amniotic Fluid microbiology, Chlamydia trachomatis, Cohort Studies, Culture Techniques, Female, Gestational Age, Humans, Interleukin-6 metabolism, Mycoplasma hominis, Polymerase Chain Reaction, Pregnancy, RNA, Ribosomal, 16S analysis, Real-Time Polymerase Chain Reaction, Retrospective Studies, Ureaplasma, Amniotic Fluid metabolism, Bacterial Infections metabolism, Cell-Free Nucleic Acids metabolism, Chorioamnionitis metabolism, DNA, Mitochondrial metabolism, Fetal Membranes, Premature Rupture metabolism, Inflammation metabolism
- Abstract
Introduction: We evaluated the levels of cell-free nuclear DNA (nDNA) and cell-free mitochondrial DNA (mtDNA) in the amniotic fluid supernatant from pregnancies complicated by preterm prelabor rupture of membranes (PPROM) based on evidence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI)., Material and Methods: A total of 155 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. The levels of cell-free nDNA and mtDNA in the amniotic fluid supernatant were assessed and quantified by real-time polymerase chain reaction., Results: The levels of cell-free nDNA and mtDNA were higher in women with MIAC and IAI than in women without these conditions (nDNA: with MIAC: median 3.9 × 10
4 genome equivalent [GE]/mL vs without MIAC: median 1.2 × 104 GE/mL, with IAI: median: 5.3 × 104 GE/mL vs without IAI: median 1.2 × 104 GE/mL; mtDNA: with MIAC: median 9.2 × 105 GE/mL vs without MIAC: median 2.5 × 105 GE/mL, with IAI: median 1.1 × 106 GE/mL vs without IAI: median 2.5 × 105 ; all P values ≤ 0.01). Women with the microbial-associated IAI showed the highest levels of cell-free nDNA and mtDNA., Conclusions: Cell-free nDNA and mtDNA are constituents of the amniotic fluid supernatant from PPROM pregnancies. Both cell-free nDNA and mtDNA are involved in the intra-amniotic inflammatory response in women with PPROM., (© 2018 John Wiley & Sons, Ltd.)- Published
- 2018
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46. Cervical human papillomavirus infection in women with preterm prelabor rupture of membranes.
- Author
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Hornychova H, Kacerovsky M, Musilova I, Pliskova L, Zemlickova H, Matejkova A, Vosmikova H, Rozkosova K, Cermakova P, Bolehovska R, Halada P, Jacobsson B, and Laco J
- Subjects
- Adult, Amniotic Fluid virology, Female, Humans, Infant, Infant Mortality, Infant, Newborn, Patient Admission, Pregnancy, Cervix Uteri virology, Fetal Membranes, Premature Rupture virology, Papillomaviridae physiology, Papillomavirus Infections complications
- Abstract
Objective: To evaluate the association between cervical human papillomavirus (HPV) infection at the time of admission and the presence of microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation (IAI) in women with preterm prelabor rupture of membranes (PPROM) and to determine the association between cervical HPV infection and short-term neonatal morbidity., Methods: One hundred women with singleton pregnancies complicated by PPROM between the gestational ages of 24+0 and 36+6 weeks were included in the study. The presence of HPV DNA was evaluated in scraped cervical cells using polymerase chain reaction (PCR). Amniotic fluid samples were obtained by transabdominal amniocentesis., Results: The rate of cervical HPV infection in women with PPROM was 24%. The rates of MIAC and IAI were not different between women with cervical HPV infection and those without cervical HPV infection [MIAC: with HPV: 21% (5/24) vs. without HPV: 22% (17/76), p = 1.00; IAI: with HPV: 21% (5/24) vs. without HPV: 18% (14/76), p = 0.77]. There were no differences in the selected aspects of short-term neonatal morbidity between women with and without cervical HPV infection., Conclusions: In women with PPROM, the presence of cervical HPV infection at the time of admission is not related to a higher risk of intra-amniotic infection-related and inflammatory complications or worse short-term neonatal outcomes., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2018
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47. Detection of cytomegalovirus DNA in fecal samples in the diagnosis of enterocolitis after allogeneic stem cell transplantation.
- Author
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Zavrelova A, Radocha J, Pliskova L, Paterova P, Vejrazkova E, Cyrany J, Gabalec F, Podhola M, and Zak P
- Subjects
- Aged, Cytomegalovirus genetics, Cytomegalovirus isolation & purification, DNA, Viral isolation & purification, Enterocolitis virology, Feces virology, Female, Hematologic Neoplasms therapy, Humans, Male, Middle Aged, Retrospective Studies, Transplantation, Homologous, Cytomegalovirus Infections diagnosis, Enterocolitis diagnosis, Stem Cell Transplantation adverse effects
- Abstract
Background: Cytomegalovirus enterocolitis is a rare but potentially life threatening complication after allogeneic stem cell transplantation. Its early diagnosis and treatment are essential for a successful outcome., Objective: To determine the potential benefit of fecal CMV DNA detection in the diagnosis of CMV colitis among stem cell transplant recipients., Study Design: Biopsies from the lower gastrointestinal tract, taken during 69 episodes of diarrhea, were compared with fecal samples previously examined for CMV DNA in 45 patients after allogeneic stem cell transplantation., Results: Six confirmed cases of CMV colitis were observed, with 16 out of 69 (23%) fecal samples proving positive for CMV DNA. Only one positive sample correlated with histologically confirmed CMV colitis, and 15 samples were evaluated as false positive. These results provide a 16.7% sensitivity and 76.2% specificity in the diagnosis of CMV enterocolitis., Conclusion: The examination of fecal samples for the presence of CMV DNA has very low potential in the diagnosis of CMV enterocolitis after allogeneic stem cell transplantation; therefore, a biopsy of the gastrointestinal mucosa is still warranted for correct diagnosis.
- Published
- 2018
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48. Gastric fluid used to assess changes during the latency period in preterm prelabor rupture of membranes.
- Author
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Musilova I, Andrys C, Hornychova H, Pliskova L, Drahosova M, Zednikova B, Bolehovska R, Faist T, Jacobsson B, and Kacerovsky M
- Subjects
- Adult, Amniocentesis, Amniotic Fluid microbiology, Biomarkers analysis, Body Fluids, Chlamydia trachomatis, Chorioamnionitis, Female, Gastric Juice microbiology, Humans, Infant, Newborn, Inflammation, Interleukin-6 analysis, Mycoplasma hominis, Pregnancy, Prospective Studies, Stomach microbiology, Syndrome, Ureaplasma, Amniotic Fluid chemistry, Fetal Blood chemistry, Fetal Membranes, Premature Rupture, Gastric Juice chemistry
- Abstract
Objective: To determine changes in the intraamniotic environment during the latency period using paired amniotic and gastric fluid samples in pregnancies complicated by preterm prelabor rupture of membranes (PPROM)., Methods: A total of 34 women with singleton pregnancies complicated by PPROM prior to 34 weeks were included in the study. Amniotic fluid was obtained by transabdominal amniocentesis at the time of admission. Immediately after delivery, umbilical cord blood and gastric fluid were obtained., Result: Microorganisms in amniotic and gastric fluid samples were found in 38% and 59% of women, respectively. Bedside IL-6 levels were higher in amniotic than in gastric fluid in pregnancies without fetal inflammatory response syndrome (FIRS) (263 pg/mL vs. 50 pg/mL; p < 0.0001), but not in pregnancies with FIRS (318 pg/mL vs. 444 pg/mL; p = 0.91). Funisitis and FIRS was associated with the highest bedside IL-6 levels in gastric fluid. A gastric fluid bedside IL-6 level of 275 pg/mL was found to be the ideal cutoff value to predict funisitis and FIRS., Conclusions: The microbial and inflammatory status of the intraamniotic compartment changes during the latency period in PPROM. Bedside IL-6 assessment of gastric fluid may be useful in the rapid diagnosis of funisitis and FIRS.
- Published
- 2018
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49. Amniotic fluid pentraxins: Potential early markers for identifying intra-amniotic inflammatory complications in preterm pre-labor rupture of membranes.
- Author
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Musilova I, Andrys C, Krejsek J, Drahosova M, Zednikova B, Pliskova L, Zemlickova H, Jacobsson B, and Kacerovsky M
- Subjects
- Adult, Amniocentesis methods, Female, Fetal Membranes, Premature Rupture metabolism, Humans, Obstetric Labor, Premature metabolism, Pregnancy, Retrospective Studies, Young Adult, Amniotic Fluid metabolism, Biomarkers metabolism, C-Reactive Protein metabolism, Inflammation metabolism, Serum Amyloid P-Component metabolism
- Abstract
In this study, pentraxin 3 (PTX3), C-reactive protein (CRP), and serum amyloid P component (SAP) concentrations in the amniotic fluid of women with preterm pre-labor rupture of membranes (PPROM) were evaluated based on evidence of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and microbial-associated IAI. A total of 149 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid PTX3, SAP, and CRP concentrations were assessed using enzyme-linked immunosorbent assay. PTX3 and CRP concentrations were higher in women with MIAC, IAI, and microbial-associated IAI than in women without these conditions. SAP concentrations were only higher in the presence of IAI and microbial-associated IAI. Amniotic fluid PTX3 concentrations of 11 ng/mL were found to be the best value for identifying the presence of microbial-associated IAI and IAI in women with PPROM. To conclude, amniotic fluid pentraxins are involved in intra-amniotic inflammatory responses in pregnancies complicated by PPROM., (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2018
- Full Text
- View/download PDF
50. Cervical fluid interleukin 6 and intra-amniotic complications of preterm prelabor rupture of membranes.
- Author
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Musilova I, Andrys C, Drahosova M, Soucek O, Pliskova L, Jacobsson B, and Kacerovsky M
- Subjects
- Adult, Amniocentesis, Amniotic Fluid microbiology, Biomarkers analysis, Chorioamnionitis diagnosis, Chorioamnionitis microbiology, Enzyme-Linked Immunosorbent Assay, Female, Fetal Membranes, Premature Rupture etiology, Fetal Membranes, Premature Rupture genetics, Fetal Membranes, Premature Rupture microbiology, Gestational Age, Humans, Infant, Newborn, Point-of-Care Testing, Polymerase Chain Reaction, Pregnancy, Prospective Studies, Ureaplasma isolation & purification, Ureaplasma Infections diagnosis, Ureaplasma Infections microbiology, Young Adult, Amnion microbiology, Amniotic Fluid chemistry, Fetal Membranes, Premature Rupture metabolism, Interleukin-6 analysis
- Abstract
Objective: To determine if cervical fluid interleukin (IL)-6 concentrations in women with preterm prelabor rupture of membranes (PPROM) allows identification of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI)., Methods: One hundred forty-four women with singleton pregnancies complicated by PPROM were included in this prospective cohort study. Cervical and amniotic fluids were collected at the time of admission and concentrations of IL-6 were measured using an ELISA and point-of-care test, respectively. Cervical fluid was obtained using a Dacron polyester swab and amniotic fluid was obtained by transabdominal amniocentesis. MIAC was diagnosed based on a positive PCR result for Ureaplasma species, M. hominis, and/or C. trachomatis and/or by positivity for the 16 S rRNA gene. IAI was defined as amniotic fluid point-of-care IL-6 concentrations ≥745 pg/mL. The women were assigned to four subgroups based on the presence of MIAC and/or IAI: microbial-associated IAI (both MIAC and IAI), sterile IAI (IAI alone), MIAC alone, and without either MIAC or IAI., Results: (1) Women with microbial-associated IAI had higher cervical fluid IL-6 concentrations (median 560 pg/mL) than did women with sterile IAI (median 303 pg/mL; p = .001), women with MIAC alone (median 135 pg/mL; p = .0004), and women without MIAC and IAI (median 180 pg/mL; p = .0001). (2) No differences were found in cervical fluid IL-6 concentrations among women with sterile IAI, with MIAC alone, and without MIAC and IAI. (3) A positive correlation was observed between cervical fluid IL-6 concentrations and the amount of Ureaplasma species in amniotic fluid (copies DNA/mL; rho = 0.57, p < .0001). (4) A weak positive correlation was detected between cervical and amniotic fluid IL-6 concentrations (rho = 0.33, p < .0001)., Conclusions: The presence of microbial-associated IAI is associated with the highest cervical fluid IL-6 concentrations. Cervical IL-6 can be helpful in the identification of microbial-associated IAI.
- Published
- 2018
- Full Text
- View/download PDF
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