Search

Nasopharyngeal carcinoma (NPC), a malignancy highly prevalent in East and Southeast Asia, is primarily treated with radiotherapy (RT). However, hypoxia-induced radioresistance presents a significant challenge. Nanozymes, nanomaterials with catalase-like activity, have emerged as a promising strategy for radiosensitization by converting elevated hydrogen peroxide in the tumor microenvironment into oxygen. Despite their potential, effectively targeting hypoxic lesions has been difficult. Here, we identify transferrin receptor 1 (TfR1) as an upregulated target in NPC, with its expression levels positively correlated with hypoxia. Human heavy-chain ferritin, a specific ligand of TfR1, selectively recognizes hypoxic NPC lesions in preclinical models. Based on these findings, we design a hypoxia-targeted nanozyme by loading platinum nanoparticles into ferritin. This nanozyme exhibits enhanced catalase-like activity and effectively alleviates tumor hypoxia in NPC xenografts. When combined with RT, a single injection of the nanozyme significantly inhibits tumor growth and prolongs mouse survival, outperforming sodium glycididazole, a clinically used radiosensitizer. In summary, our findings highlight TfR1 as an accessible cell surface target in hypoxic NPC lesions. The nanozyme targeting TfR1 holds promise for enhancing the therapeutic effectiveness of RT in NPC through an in situ oxygen-generation mechanism., Competing Interests: Competing interests: The authors declare no competing interests., (© 2025. The Author(s).)

The stimulator of interferon genes (STING) pathway exhibits great potential in remodeling the immunosuppressive tumor microenvironment and initiating antitumor immunity. However, how to effectively activate STING and avoid undesired toxicity after systemic administration remains challenging. Herein, platinum(IV)-backboned polymer prodrug-coated manganese oxide nanoparticles (DHP/MnO 2 NP) with pH/redox dual responsive properties are developed to precisely release cisplatin and Mn 2+ in the tumor microenvironment and synergistically amplify STING activation. In vitro , we demonstrate that DHP/MnO 2 NP can effectively induce tumor cell DNA damage and leak into the cytoplasm, cooperating with Mn 2+ to promote STING activation and significantly upregulate the expression of proinflammatory cytokines. Additionally, DHP/MnO 2 NP can selectively release cisplatin and Mn 2+ to mediate tumor killing while reducing toxicity to normal cells. In vivo , DHP/MnO 2 NP exerted increased therapeutic efficacy by inducing STING activation and initiating robust antitumor immunity. Specifically, DHP/MnO 2 NP effectively skewed tumor-associated macrophages toward a proinflammatory phenotype and upregulated the expression of proinflammatory cytokines in tumors by up to 99-fold relative to the control. And the infiltration of CD8 + T cells was also significantly increased. When STING signaling was blocked, the antitumor effects and immunostimulatory efficacy of DHP/MnO 2 NP were significantly inhibited. Moreover, DHP/MnO 2 NP possess the advantage of enhanced tumor homing and retention, resulting in stronger and longer-lasting anticancer effects. Overall, DHP/MnO 2 NP provide a potential platform for potentiating cancer chemoimmunotherapy and hold promise for precision treatment.

Pyroptosis, an inflammatory modality of programmed cell death associated with the immune response, can be initiated by bioactive ions and reactive oxygen species (ROS). However, bioactive ion-induced pyroptosis lacks specificity, and further exploration of other ions that can induce pyroptosis in cancer cells is needed. Sonocatalytic therapy (SCT) holds promise due to its exceptional penetration depth; however, the rapid recombination of electron-hole (e - -h + ) pairs and the complex tumor microenvironment (TME) impede its broader application. Herein, we discovered that antimony (Sb)-based nanomaterials induced pyroptosis in cancer cells. Therefore, Schottky heterojunctions containing Sb component (Sb 2 Se 3 @Pt) were effectively designed and constructed via in situ growth of platinum (Pt) nanoparticles (NPs) on Sb 2 Se 3 semiconductor with narrow band gaps, which were utilized as US-heightened pyroptosis initiators to induce highly effective pyroptosis in cancer cells to boost SCT-immunotherapy. Under US irradiation, excited electrons were transferred from Sb 2 Se 3 nanorods (NRs) to the co-catalyst Pt via Schottky junctions, and band bending effectively prevented electron backflow and achieved efficient ROS generation. Moreover, the pores oxidized and depleted the overexpressed GSH in the TME, potentially amplifying ROS generation. The biological effects of the Sb 2 Se 3 @Pt nanoheterojunction itself combined with the sonocatalytic amplification of oxidative stress significantly induced Caspase-1/GSDMD-dependent pyroptosis in cancer cells. Therefore, SCT treatment with Sb 2 Se 3 @Pt not only effectively restrained tumor proliferation but also induced potent immune memory responses and suppressed tumor recurrence. Furthermore, the integration of this innovative strategy with immune checkpoint blockade (ICB) treatment elicited a systemic immune response, effectively augmenting therapeutic effects and impeding the growth of abscopal tumors. Overall, this study provides further opportunities to explore pyroptosis-mediated SCT-immunotherapy., (© 2024 Wiley-VCH GmbH.)

Pain and inflammation are common symptoms of a majority of the diseases. Chronic pain and inflammation, as well as related dreadful disorders, remain difficult to control due to a lack of safe and effective medications. In this work, biocompatible platinum nanoparticles with significant analgesic and anti-inflammatory action were synthesized through a wet chemical method using polyethylene glycol-400 as a capping agent and sodium borohydride as a reducing agent. The average particle size of these Pt nanospheres was determined to be 3.26 nm using TEM analysis, and X-ray diffraction confirmed their face-centered cubic crystalline structure. Fourier transform infrared and UV-visible spectroscopy confirm that Pt-NPs are coated with the PEG-400 molecule. The significantly negative zeta potential value (-26.8 mV) indicates the stability of the produced nanoparticles. In vitro cytotoxicity studies on normal cell lines show nontoxic behavior with over 96% cell viability at 100 μg/mL of the test sample. In vitro assays of inhibition of protein denaturation and DPPH free radical scavenging elucidated the anti-inflammatory and antioxidant properties of PEGylated Pt NPs with promising EC 50 values 57.99 and 9.324 μg/mL, respectively. In vivo animal trials confirmed that PEG-capped Pt-NPs are more effective than conventional medicines. The in vivo hot plate assay for the analgesic study shows a maximum response time of 14.5 ± 1.22 s (92.54% analgesia) at a dosage of 50 mg/kg and 13.8 ± 0.71 s (86.05% analgesia) at a dosage of 25 mg/kg after 180 and 240 min of administration, respectively. In the rat paw edema model for anti-inflammatory activity, the PEG-capped Pt NPs exhibit significant inhibitory action, with the maximum percentage of edema inhibition at a dosage of 50 mg/kg identical to that of the aspirin-based standard medication administered at a higher dosage of 100 mg/kg, resulting in 42% inhibition, suggesting a versatile solution for inflammation and persistent pain.

Proteins are generally resistant to large conformational changes under physiological conditions. Here, we show that platinum (Pt(II)), which is widely-used metal centre in cancer therapeutic drugs, binds to a cytosolic protein, small ubiquitin-like modifier 1 (SUMO1), under physiological conditions and changes its conformation to a molten globule (MG). Mass spectrometry (ICP-MS) studies confirmed stoichiometric Pt(II) binding to SUMO1. Fluorescence spectroscopy showed Tyr fluorescence quenching and increased ANS binding. Fluorescence assays on Trp-mutants indicated conformational changes and circular dichroism (CD) suggested MG formation upon Pt(II) binding. In contrast, structural homologues of SUMO1 (ubiquitin (Ubq) and SUMO2) showed no conformational changes on Pt(II) titration. Further studies compared the impact of distinct His residues in SUMO1 on Pt(II) binding and protein structure to SUMO2 and Ubq. Experiments at low pH (5.0) implicated His residues interacting with Pt(II), corroborated by the absence of conformational change in the H75L mutant of SUMO1. Pt(II)-His binding in SUMO1 unravels key molecular determinants of Pt(II)-protein interactions and their conformational consequences. SUMO1 with other SUMOylation components are shown to have enhanced expression in several cancers, hence, our study suggests a possible fate of the non-targetability of Pt(II)-based drugs on SUMOylation in cancer cells, upon interaction with SUMO1., (© 2024 The Authors. Chemistry - An Asian Journal published by Wiley-VCH GmbH.)

Sensitive and accurate determination of acetamiprid is highly desirable for guaranteeing food safety. In this Letter, an energy-transfer-based dual-mode biosensor was developed using zinc-based metal-organic frameworks (Zn-MOFs) acting as both photoelectrochemical (PEC) and electrochemiluminescent (ECL) donors and Pt@Cu 2 O cubic nanocrystals (CNs) as the energy acceptor for detecting acetamiprid. By integration of aptamer recognition with two-step DNA circuit amplification (entropy-driven DNA cycle and DNA walker), the detection of acetamiprid was converted into the assay of abundant intermediate DNA strands. With the help of nicking endonuclease, a large number of single-stranded DNAs was generated on the surface of Zn-MOFs, which were used as multifunctional PEC and ECL substrates. Through competitive hybridization, Pt@Cu 2 O CNs as broad-spectrum quenchers were introduced, thereby enabling changes in the PEC and ECL responses for acetamiprid quantitation. The experimental results proved that the combination of energy transfer, two-step DNA circuit amplification, and dual-mode sensing strategy achieved the sensitive and accurate analysis of acetamiprid, with low detection limits of 20.2 fM (PEC mode) and 17.5 fM (ECL mode) within a wide range from 0 to 1 × 10 -9 M. The excellent specificity, reproducibility, and practicality confirmed the potential application of the biosensor for pesticide-related food safety.

Photodynamic therapy (PDT) and catalytic therapy were promising treatment modes, but tumor hypoxia and poor catalytic activity severely limited their efficacies. Herein, using a porphyrin metal-organic framework (PCN-224) as nanocarrier, a platinum/palladium (Pt/Pd) dual-modified PCN-224 nanoprobe (PCN-224-Pt@Pd) with strong peroxidase (POD)/catalase (CAT)-like activities was developed, achieving photothermal-promoted PDT/catalytic therapy. Compared with single ultrasmall Pt modifying, CAT-like activity of Pt/Pd dual-modifying increased oxygen concentration from 6.24 to 9.35 mg/L, which improved singlet oxygen ( 1 O 2 ) yield from 63.8 % to 82.9 %. Moreover, POD-like activity of Pt/Pd dual-modifying significantly accelerated hydroxyl radicals (·OH) generation. Importantly, PCN-224-Pt@Pd possessed near-infrared II (NIR-II) photothermal effect with a high efficiency (55.6 %), which further promoted ·OH production. Under combined therapy of PCN-224-Pt@Pd, the cell survival rate greatly reduced to 5.8 %, and the tumors were cured, suggesting NIR-II photothermal-enhanced PDT/catalytic therapy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Background: Breast cancer CTCs have recently been recognized as an emerging biomarker for liquid biopsy of breast cancer. In this work, based on two-dimensional (2D) noble metal PtCo@rGO nanozymes and Au@CNTs bioconjugates, a novel electrochemiluminescence (ECL) cytosensor was developed in order to detect breast cancer CTCs (MCF-7) ultrasensitively., Results: The PtCo@rGO nanozymes possessed large specific surface area and high efficiency peroxidase-like activity, which can be used as nanocarriers to anchor and catalyze luminol ECL emission efficiently. Moreover, the PtCo@rGO nanozymes have fractal nanostructures similar to that of CTCs and can capable of enhancing the adhesion of MCF-7 when assembled together with aptamers containing HS-modified epithelial specific cell adhesion molecules (EpCAM, S1). Importantly, the S1/Au@CNTs bioconjugates loaded on the glassy carbon electrode (GCE) can effectively capture MCF-7 cells. Benefiting from the above-mentioned advantages, the ECL cytosensor constructed for MCF-7 cells detection performed well with a wide linear range (2-1 × 10 4  cells mL -1 ) and a low limit of detection (1 cells mL -1 )., Significance: The designed ECL cytosensor could provide a promising platform for CTC-based liquid biopsy and have broad application prospects in breast cancer early diagnosis and prognostic monitoring., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Controlling the size and morphology of supramolecular assemblies is still an enormous challenge for the development of novel functional materials. Herein, cyclometalated platinum(II) complexes were designed and used as effective theragnostic supramolecular nano agents. Benzoquinoline was used as the main C^N ligand, and the increased number of aromatic rings in the N^N ligand of Pt complexes could greatly improve lipophilicity and cytotoxicity towards cancer cells over normal cells. Moreover, the morphology of nanoparticles formed by self-assembly of Pta-d could change from one to three-dimensional (1-3D), which can form nanowires and nanospheres. Besides, complexes of Pta-c, the number of aromatic rings in the N^N ligand of which does not exceed 4, all exhibit significant aggregation-induced phosphorescent emission (AIPE) with 100 - 200-fold enhancement in red phosphoresce intensity recorded in the solvent of Pta-1 and Ptb. Pt-Pt interaction induced by coordination and electrostatic interaction between complex and anions, and a new deep red emission improvement was observed in aqueous solution of Pta and Ptb with the presence of ClO 4 - , and two similar deep red emissions induced by two different interactions can be told by new emerging MMLCT absorption band. Ptd of dppz ligand exhibits the highest efficiency in inducing apoptosis of HeLa and its anticancer mechanism was studied. Our work aims to promote the fundamental comprehension of the self-assembly behavior of cyclometalated platinum complex with AIPE in vitro and living cells., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Background: Respiratory illnesses stemming from influenza A viruses represent a significant worldwide health concern. There is an immediate need for a rapid and sensitive method to detect influenza A viruses early, without requiring extra equipment., Results: Here, we established a lateral flow immunoassay (LFIA) for the detection of influenza A (Flu A) using a "three-in-one" multifunctional mesoporous Fe 3 O 4 @SiO 2 @Pt nanozymes (Fe 3 O 4 @MSiO 2 @Pt NZs) with excellent magnetic separation properties, colorimetric, and peroxidase-like (POD-like) activities. Effective enrichment of target Flu A in complex samples as well as greater loading of Pt particles by mesoporous structures with large specific surface area to enhance POD-like activity can significantly improve the detection sensitivity of the LFIA. After colorimetric amplification by Fe 3 O 4 @MSiO 2 @Pt tags catalysis, the qualitative and quantitative results of detection for Flu A nucleoprotein (Flu A-NP) were 0.01 and 0.0089 ng mL -1 , respectively. This indicated a sensitivity approximately 100 times greater than commercially available colloidal Au nanoparticle (AuNP)-based LFIA strips. For detection of inactivated H1N1 virus, quantification can be as low as 33 copies mL -1 . Moreover, it demonstrated high accuracy in pharyngeal swab sample simulation experiments., Significance: Therefore, the proposed platform based on Fe 3 O 4 @MSiO 2 @Pt NZs-LFIA offered a promising approach for point-of-care testing (POCT), enabling rapid and ultrasensitive diagnosis of Flu A., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Rapid and accurate detection of Burkholderia gladioli (B. gladioli) and effective sterilization are crucial for ensuring food safety. Hence, a novel "loong frolic pearls" platform based on platinum-based fluorescent nanozymes (Pt-OCDs) and strand exchange amplification (SEA) was reported. Magnetic nanoparticles were modified on primer SEAF, while Pt-OCDs were covalently coupled with primer SEA-R. The highly efficient amplification capability of SEA permitted the accumulation of a large number of double-labeled amplicons. After magnetic adsorption, the supernatant was detected in reverse direction to collect colorimetric-fluorescence-photothermal signal values, enabling ultra-precise detection within 1 h. Furthermore, the Pt-based multifunctional nanoplatform generated abundant •OH and 1 O 2 , which synergistically attacked B. gladioli and its biofilm, resulting in significant bactericidal efficacy within 30 min. This "triple-detection and double-sterilization" platform has been successfully applied in the field of food analysis with good recovery rates and immediate control over B. gladioli, thus demonstrating promising prospects for broad applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Bacterial biofilms are complex multicellular communities that adhere firmly to solid surfaces. They are widely recognized as major threats to human health, contributing to issues such as persistent infections on medical implants and severe contamination in drinking water systems. As a potential treatment for biofilms, this work proposes two strategies: (i) light-driven ZnFe 2 O 4 (ZFO)/Pt microrobots for photodegradation of biofilms and (ii) magnetically driven ZFO microrobots for mechanical removal of biofilms from surfaces. Magnetically driven ZFO microrobots were realized by synthesizing ZFO microspheres through a low-cost and large-scale hydrothermal synthesis, followed by a calcination process. Then, a Pt layer was deposited on the surface of the ZFO microspheres to break their symmetry, resulting in self-propelled light-driven Janus ZFO/Pt microrobots. Light-driven ZFO/Pt microrobots exhibited active locomotion under UV light irradiation and controllable motion in terms of "stop and go" features. Magnetically driven ZFO microrobots were capable of maneuvering precisely when subjected to an external rotating magnetic field. These microrobots could eliminate Gram-negative Escherichia coli ( E. coli ) biofilms through photogenerated reactive oxygen species (ROS)-related antibacterial properties in combination with their light-powered active locomotion, accelerating the mass transfer to remove biofilms more effectively in water. Moreover, the actuation of magnetically driven ZFO microrobots allowed for the physical disruption of biofilms, which represents a reliable alternative to photocatalysis for the removal of strongly anchored biofilms in confined spaces. With their versatile characteristics, the envisioned microrobots highlight a significant potential for biofilm removal with high efficacy in both open and confined spaces, such as the pipelines of industrial plants.

The integration of a photosensitive gate into an organic electrochemical transistor has currently emerged as a promising route for biological sensing. However, the modification of the photosensitive gate always involves complex processes, and the degradation of sensitivity of the functional materials under illumination will significantly decrease the stability of the devices. Herein, we designed an organic photoelectrochemical transistor (OPECT) biosensor employing horseradish peroxidase (HRP)@glucose oxidase (GOx)/Pt/n-Si as the photosensitive gate based on the "shadow effect". The glucose-dependent hydrogen peroxide with HRP/GOx was modified on the gate electrode, triggering a biocatalytic precipitation reaction, which induces the illumination contrast, resulting in a biologically gating effect on the corresponding channel current response. Thus, high sensitivity and selectivity in glucose detection of the OPECT devices will be realized. Given the easy fabrication and high stability of the Pt/n-Si electrode, it has great potential to become a superior selectivity as an OPECT gate electrode. This work provides conceptual validation for the study of the interaction between the photosensitive gate based on the "shadow effect" and biomolecular sensing, which can further expand the application of the OPECT biosensors under interior lighting and shadow surroundings.

The field of platinum chemistry is ubiquitous in the research of anticancer drugs and new OLED materials. Within the vast library of existing compounds, the majority of work focuses on complexes in the +2 and +4 oxidation states, with comparatively few examples of Pt III complexes reported without bridging ligands. Pt III complexes with metal-metal bonding can be made by mild oxidation of Pt II complexes having bis(phenylpyridine) ligands. Here, we report the synthesis and characterization of two new Pt II complexes bearing tetradentate N-bridged bis(phenylpyridine) ligands with N^C*C^N and C^N*N^C coordinating atoms. Through chemical oxidation with PhICl 2 and N-chlorosuccinimide, we obtained four new complexes containing Pt IV and Pt III centres. The dynamics of the Pt III  complexes were explored by variable-temperature NMR studies and showed surprisingly free rotation of the appended N-aryl group. This work uncovers the importance of the destabilizing nature of the bulky N-aryl group on intermetallic bonds, and sheds light on how the molecular rotation observed in the Pt III compounds could inspire new molecular rotors driven by intermetallic bonds., (© 2025 Wiley-VCH GmbH.)

The emerged apoptosis/ferroptosis synergistic platinum-based therapy has attracted a lot of attention but is far from clinic use due to high systemic toxicity. Herein, a series of novel precise carrier-free self-assembled platinum(IV) nanoparticles with lipid regulation effect named FSPNPs ( 5 NPs- 8 NPs) were constructed via connecting fenofibrate acid (FA) to cisplatin or oxaliplatin-derived platinum(IV)-intermediates with disulfide bonds. FSPNPs can be stimulated by high-glutathione/ascorbic acid and acidity environment to produce an "explosion-like" cascade release process. Cell-activity showed precision of FSPNPs, which accumulated more in tumor cells and inhibited cell proliferation. Especially, 5 NPs have higher cell selectivity than cisplatin. FSPNPs downregulated glutathione/glutathione peroxidase 4, increased reactive oxygen species/lipid peroxidation/malondialdehyde, induced DNA damage/S-phase arrest, and regulated p53/Bcl-2/Bax to trigger the apoptosis/ferroptosis hybrid pathway. The released FA and derivates were docked into the peroxisome proliferator-activated receptor α with activating cholesterol metabolism to destroy membrane integrity. FSPNPs also showed good biocompatibility and superior antitumor activity with no observable tissue damage.

Carbendazim (CBZ) is used to prevent fungal infections in agricultural crops. Given its high persistence and potential for long-term health effects, it is crucial to quickly identify pesticide residues in food and the environment in order to mitigate excessive exposure. Aptamer-based sensors offer a promising solution for pesticide detection due to their exceptional selectivity, design versatility, ease of use, and affordability. Herein, we report the development of an electrochemical aptasensor for CBZ detection. The sensor was fabricated through a one-step electrodeposition of platinum nanoparticles (Pt NPs) and reduced graphene oxide (rGO) on a glassy carbon electrode (GCE). Then, a CBZ-specific aptamer was attached via Pt-sulfur bonds. Upon combining CBZ with the aptamer on the electrode surface, the redox reaction of the electrochemical probe K 4 [Fe(CN) 6 ] is hindered, resulting in a current drop. Under optimized conditions (pH of 7.5 and 25 min of incubation time), the proposed aptasensor showed a linear current reduction to CBZ concentrations between 0.5 and 15 nM. The limit of detection (LOD) for this proposed aptasensor is 0.41 nM. Along with its repeatable character, the aptasensor demonstrated better selectivity for CBZ compared to other potential compounds. The recovery rates for detecting CBZ in skim milk and tap water using the standard addition method were 98% and 96%, respectively. The proposed aptasensor demonstrated simplicity, sensitivity, and selectivity for detecting CBZ with satisfactory repeatability. It establishes a strong foundation for environmental monitoring of CBZ.

A nano-enzyme sandwich assay (SWzyme assay), a colorimetric system based on a biochip and inorganic nano-enzyme for rapid and simple determination of exosomal Aβ42 in plasma is proposed. Anti-CD63 antibody-modified biochips were prepared for plasma exosome capture and synthesized highly catalytic Ni@Pt nanozymes for detecting exosomal Aβ42. The method was able to detect exosomal Aβ42 with a limit of detection (LOD) as low as 4.2×10 4 particles/mL and a linear range from 10 4 to 10 8 particles/mL. By determination of exosomal Aβ42, the SWzyme assay successfully distinguished plasma from Alzheimer's desease (AD) and healthy mice. The SWzyme assay holds promise to serve as diagnostic tools for the early detection of AD and supporting the development of personalized medicine., Competing Interests: Declarations. Ethical approval: All animal experiments were performed according to protocols approved by the Life Sciences Ethics Review Committee of Zhengzhou University. Competing interests: The authors declare no competing financial interest., (© 2025. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Rationale: Immunogenic cell death (ICD) offers a promising avenue for the treatment of triple-negative breast cancer (TNBC). However, optimizing immune responses remains a formidable challenge. This study presents the design of RBCm@Pt-CoNi layered double hydroxide (RmPLH), an innovative sonosensitizer for sonodynamic therapy (SDT), aimed at enhancing the efficacy of programmed cell death protein 1 (PD-1) inhibitors by inducing robust ICD responses. Methods: Pt-CoNi layered double hydroxide (LDH) nanocages were synthesized using a two-step method, followed by functionalization with red blood cell membranes to prepare RmPLH. The in vitro assessments included evaluations of cell toxicity, cellular uptake, and sonodynamic effects of RmPLH. Key mechanisms-such as oxidative stress, DNA damage, pyroptosis, cGAS/STING pathway activation, and inhibition of cellular migration and invasion-were explored under varying treatment conditions in 4T1 cells. Tumor-bearing mice were employed to evaluate tumor-targeting capabilities and the synergistic tumor-suppressive effects of RmPLH combined with PD-1 inhibitors. Comprehensive safety evaluations, including blood tests, biochemical analyses, and histopathological examinations, were also conducted. Results: The synthesized Pt-CoNi LDH exhibited a uniform rhombic dodecahedral nanocage morphology with an average particle size of approximately 231 nm. Encapsulation with red blood cell membranes conferred prolonged systemic circulation, enhanced tumor targeting, and reduced immune clearance for RmPLH. Upon ultrasound (US) stimulation, the LDH released substantial levels of reactive oxygen species (ROS) and platinum ions. The ROS effectively induced endoplasmic reticulum stress and ferroptosis, while platinum ions facilitated DNA crosslinking, triggering significant DNA damage. ROS-induced pyroptosis released inflammatory mediators and damage-associated molecular patterns (DAMPs), which activated the cGAS/STING pathway and reinforced ICD. Combining RmPLH with PD-1 inhibitors significantly enhanced therapeutic efficacy against TNBC. Furthermore, safety assessments confirmed the excellent biocompatibility and biosafety of RmPLH . Conclusion: The integration of RmPLH with PD-1 inhibitors substantially amplifies ICD, fostering robust antigen-specific T cell immunity and offering a promising therapeutic strategy for TNBC. This study represents a pioneering application of Pt (II)-based LDH nanocages in oncology, laying a foundation for future innovations in tumor immunotherapy., Competing Interests: Competing Interests: The authors have declared that no competing interest exists., (© The author(s).)

As a vital factor in cell metabolism, nitric oxide (NO) is associated with nitrosative stress and subsequent inflammations and diseases. In situ, real-time NO monitoring is challenging due to its relative trace concentration and fast diffusion in cell. Scanning electrochemical microscopy (SECM) is suited uniquely for single-cell analysis, and its electrochemical response to targets can be further enhanced by improving the interfacial properties of its tip. Here, an ultramicroelectrodes (UMEs) modification strategy based on bimetallic single-particle was proposed for the first time. This mesoporous platinum/iridium alloy single-particle (mPtIr SP) interface using micelle-assisted electrodeposition was characterized by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). And the nucleation kinetic progress which can be defined as "oil-in-water-like" electrodeposition was discussed in detail. The high sensitivity (203.86 μA/μM·cm 2 ) and good selectivity for NO detection benefits from the high catalysis of the PtIr alloy and the high mass transfer properties of the porous interface. In particular, this novel UME can real-time monitor NO release from a single MCF-7 cell stimulated by perfluorooctanoic acid (PFOA), providing new ideas for contaminant toxicity assessment, health diagnostics, and disease treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

With the deepening understanding of diseases, increasing attention has been paid to personalized healthcare and precise diagnosis, which usually depend on the simultaneous monitoring of multiple metabolites, therefore requiring biological sensing systems to possess high sensitivity, specificity, throughput, and instant monitoring capabilities. In this work, we demonstrated the active-matrix extended-gate field-effect transistor (AMEGFET) array that can perform instant analysis of various metabolites in small amounts of body fluids collected during routine physiological activities. The extended gate electrodes of the AMEGFETs comprise ordered mesoporous carbon fibers loaded with both oxidoreductase enzymes for specific metabolites and platinum nanoparticles. By selecting customized electrode combinations, the AMEGFET array can monitor the concentrations of metabolites closely associated with chronic diseases and lifestyles, such as glucose, uric acid, cholesterol, ethanol, and lactate. The switch function of AMEGFET not only simplifies the readout circuitry for large-scale arrays but also avoids the mutual interferences among sensing units. The high flexibility and scalability make the AMEGFET array widely applicable in establishing high-throughput sensing platforms for biomarkers, providing highly efficient technical support for proactive health and intelligent healthcare., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

Serotonin is an essential neurotransmitter that regulates many physiological processes and is related to a variety of diseases. Herein, a novel electrochemiluminescence-resonance energy transfer (ECL-RET) aptasensor for serotonin detection was developed, with zinc-based metal-organic frameworks (Zn-MOFs) as an ECL donor and Pt@Cu 2 O cubic nanocrystals (CNs) as an acceptor. In the presence of target, numerous Pt@Cu 2 O CNs were brought to electrode surface through the catalytic hairpin assembly (CHA)-driven DNA walker, resulting in a significant inhibition of ECL signal. The efficient ECL-RET device exhibited a wide linear range for monitoring serotonin (10 -12 to 10 -6  M) and a low detection limit of 0.5 pM. Furthermore, satisfactory recoveries were obtained by using the aptasensor to monitor serotonin levels in serum and urine samples. The broadband absorption feature of Pt@Cu 2 O CNs, along with the extraordinary amplification effect of catalytic hairpin assembly (CHA)-driven DNA walking machine, provided a new route for the construction of efficient ECL-RET systems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Microelectrode arrays (MEAs) are pivotal brain-machine interface devices that facilitate in situ and real-time detection of neurophysiological signals and neurotransmitter data within the brain. These capabilities are essential for understanding neural system functions, treating brain disorders, and developing advanced brain-machine interfaces. To enhance the performance of MEAs, this study developed a crosslinked hydrogel coating of calcium alginate (CA) and chitosan (CS) loaded with the anti-inflammatory drug dexamethasone sodium phosphate (DSP). By modifying the MEAs with this hydrogel and various conductive nanomaterials, including platinum nanoparticles (PtNPs) and poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT: PSS), the electrical properties and biocompatibility of the electrodes were optimized. The hydrogel coating matches the mechanical properties of brain tissue more effectively and, by actively releasing anti-inflammatory drugs, significantly reduces post-implantation tissue inflammation, extends the electrodes' lifespan, and enhances the quality of neural activity detection. Additionally, this modification ensures high sensitivity and specificity in the detection of dopamine (DA), displaying high-quality dual-mode neural activity during in vivo testing and revealing significant functional differences between neuron types under various physiological states (anesthetized and awake). Overall, this study showcases the significant application value of bioactive hydrogels as excellent nanobiointerfaces and drug delivery carriers for long-term neural monitoring. This approach has the potential to enhance the functionality and acceptance of brain-machine interface devices in medical practice and has profound implications for future neuroscience research and the development of strategies for treating neurological diseases., Competing Interests: Declaration of competing interest The authors declare they have no competing interests., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The development of more sensitive, stable, and portable biosensors is crucial for meeting the growing demands of diverse and complex detection environments. MOF-based nanozymes have emerged as excellent optical reporters, making them ideal signal donors for constructing multi-signal lateral flow immunoassays (LFIA). In this study, a ZrFe-MOF@PtNPs nanocomposite was synthesized by uniformly depositing platinum nanoparticles (PtNPs) onto the surface of ZrFe-MOFs using an impregnation-reduction method. The ZrFe-MOF@PtNPs exhibited broad absorption spectra, excellent peroxidase-like activity (SA = 21.77 U/mg), high solvent stability, and efficient antibody binding capability. A portable LFIA platform was developed based on ZrFe-MOF@PtNPs and a smartphone for the targeted detection of carcinogenic aflatoxins. This method enabled the readout of colorimetric, fluorescent, and catalytic signals, significantly enhancing detection sensitivity, ensuring result accuracy, and expanding the dynamic detection range. For aflatoxin M1, the calculation of the detection limit of the three signal modes reached as low as 0.0062 ng/mL, which is two orders of magnitude more sensitive than AuNPs-LFIA (0.1839 ng/mL). This study provides effective guidance for multifunctional modification of MOFs and serves as a reference for the application of MOF-based nanozymes in point-of-care biosensors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

With the increase of antimicrobial resistance, rapid antibiotic susceptibility testing (AST) to guide precise antibiotic administration has become increasingly important. However, current gold standard AST approaches tend to take up to 24-48 h. In this work, based on the nature of catalase-positive bacteria decomposing H 2 O 2 , we developed a rapid, portable, straightforward, and cost-effective phenotypic AST approach by detecting residual H 2 O 2 using a Pt nanoparticles-based electrochemical sensor. The pulse current of the sensor exhibited a linear increase with rising H 2 O 2 concentration, demonstrating a high sensitivity of ∼382.2 μA cm -2  mM -1 . This approach showed superb diagnostic performance, with an area under the curve of 1 for 24 clinical samples of Escherichia coli and Staphylococcus aureus, with a total detection time of 60 and 45 min, respectively. Furthermore, the performance of the sensor showed no degradation even after 100 detections, promising a substantial reduction in AST costs. Overall, the proposed approach exhibited immense potential for diagnosing bacterial antibiotic resistance., Competing Interests: Declaration of competing interest The authors declared that they have no conflicts of interest to this work. We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Nerve injury represents the primary reason of mortality and disability in ischemic stroke, but effective drug delivery to the region of cerebral ischemia and hypoxia poses a significant challenge in neuroprotective treatment. To address these clinical challenges, a biomimetic nanomotor, Pt@LF is designed, to facilitate deep delivery of neuroprotective agents and inhibit ferroptosis in ischemic stroke. Pt@LF traverses the blood-brain barrier (BBB) and penetrates into deep cerebral ischemic-hypoxic areas due to the active targeting capacity of apo-lactoferrin (Apo-LF) and the self-propelling motion properties of nanomotors. Subsequently, Pt@LF loosens thrombus and alleviates the "no reflow" phenomenon via mechanical thrombolysis. Thanks to the various enzyme-like abilities and multi-target ferroptosis inhibition capability, Pt@LF ameliorates the inflammatory microenvironment and rescues dying neurons. In conclusion, Pt@LF demonstrates efficiently deep penetration and neuroprotective effects in vitro and vivo. And this study provides a promising therapeutic platform for the treatment of ischemic stroke., (© 2024 Wiley‐VCH GmbH.)

The tumor microenvironment (TME) is characterized by hypoxia and low immunogenicity, with a dense and rigid extracellular matrix (ECM) that impedes the diffusion of therapeutic agents and immune cells, thereby limiting the efficacy of immunotherapy. To overcome these challenges, an oxygen defect piezoelectric-photothermal sensitizer, bismuth vanadate nanorod-supported platinum nanodots (BVP) is developed. The integration of platinum enhances the photothermal effect and improves charge separation efficiency under ultrasound, leading to increased heat generation and the production of reactive oxygen species (ROS) and oxygen. Platinum also catalyzes the conversion of hydrogen peroxide in the TME to oxygen, which serves as both a ROS source and a means to alleviate tumor hypoxia, thereby reversing the immunosuppressive TME. Moreover, the coordination of bismuth ions with glutathione further amplifies cellular oxidative stress. The generated heat and ROS not only denature the collagen in the ECM, facilitating the deeper penetration of BVP into the tumor but also induce immunogenic cell death in tumor cells. Through the "degeneration and penetration" strategy, photoacoustic therapy effectively activates immune cells, inhibiting both tumor growth and metastasis. This study introduces a pioneering approach in the design of antitumor nanomedicines aimed at reversing the immunosuppressive characteristics of the TME., (© 2024 Wiley‐VCH GmbH.)

Clinical immune checkpoint blockade (ICB)-based immunotherapy of malignant tumors only elicits durable responses in a minority of patients, primarily due to the highly immunosuppressive tumor microenvironment. Although inducing immunogenic cell death (ICD) through reactive oxygen biocatalyst represents an attractive therapeutic strategy to amplify ICB, currently reported biocatalysts encounter insurmountable challenges in achieving high ROS-generating activity to induce potent ICD. Here, inspired by the natural catalytic characteristics of NADPH oxidases, the design of efficient, robust, and electron-rich Pt-based redox centers on the non-stoichiometric W 18 O 49 substrates (Pt─WO x ) to serve as bioinspired reactive oxygen biocatalysts to potently activate the ICD, which eventually enhance cancer immune responses and amplifies the ICB-based immunotherapy is reported. These studies demonstrate that the Pt─WO x exhibits rapid electron transfer capability and can promote the formation of electron-rich and low oxophilic Pt redox centers for superior reactive oxygen biocatalysis, which enables the Pt─WO x -based inducers to trigger endoplasmic reticulum stress directly and stimulate immune responses potently for amplifying the anti-PD-L1-based ICB therapy. This bioinspired design provides a straightforward strategy to engineer efficient, robust, and electron-rich reactive oxygen biocatalysts and also opens up a new avenue to create efficient ICD inducers for primary/metastatic tumor treatments., (© 2024 Wiley‐VCH GmbH.)

Chiral Au@Pt nanoparticles (NPs) with optically plasmonic and catalytic active surfaces were sustainably prepared to serve as label-free surface enhanced Raman scattering (SERS) platform to distinguish D- and L-enantiomers of alanine and tartaric acid. Surface morphologies were characterized by high-angle annular dark-field imaging-scanning transmission electron microscopy (HADDF-STEM) and selected area energy diffraction (SAED) patterns. The amounts of Pt on chiral Au NPs were estimated by the inductively coupled plasma-optical emission spectrometer (ICP-OES) and X-ray diffraction (XRD). The versatile applications including the environmental hazardous species monitoring and the fundamental enantiomeric differentiation are demonstrated for the novelty of prepared materials. Au@Pt NPs were used as a unique platform of H 2 O 2 and Hg 2+ concentrations using UV-Vis absorption and surface enhanced Raman scattering (SERS). The chiral Au@Pt NP platform may provide an efficient approach to achieve chiral nanocatalysis for numerous chemical reactions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Nanomotors have emerged as promising candidates for the deep penetration of loaded drugs into cancer stem cells (CSCs) located within the core of tumor tissues. A crucial factor in maximizing the clinical potential of nanomotors lies in their ability to respond dynamically to various stimuli in the tumor microenvironment. By adjusting their propulsion mechanisms in response to various stimuli, nanomotors can maintain directional movement, thus improving drug distribution and therapeutic efficacy. In this study, we present the design of a pH-responsive multi-phoretic propelled Janus nanomotor, comprising a SiO 2 @Pt core@shell nanosphere and half-wrapped acrylic acid polymers (PAA)-conjugated gold (Au) nanoparticles (JMSNs@Pt@P-Au). The JMSNs@Pt@P-Au catalyze endogenous H 2 O 2 into O 2 , propelling the nanomotors into solid tumors. Within the tumor microenvironment, the contraction of PAA triggers contact between the Au and Pt layers, facilitating self-electrophoresis propulsion. Simultaneously, a local thermal gradient is generated on the Au layer under near-infrared light irradiation, propelling the nanomotor through thermophoresis. Exploiting the unique structure of JMSNs@Pt@P-Au, the driving forces generated by H 2 O 2 catalysis, self-electrophoresis, and thermophoresis exhibit consistent motion directions. This consistency not only provides thrust for deep penetration but also enhances their targeted therapeutic efficiency against CSCs in vivo. This combination of nanomotor-driven power sources holds significant potential for designing intelligent, active drug delivery systems for effective CSC-targeted cancer therapy. STATEMENT OF SIGNIFICANCE: Deep penetration of nanomedicine in solid tumor tissue and cells is still an important challenge that restricts the therapeutic effect. Multiple-propelled nanomotors have been confirmed to be self-propulsive that overcome the limited penetration in solid tumor. However, their effective translation toward clinical applications is limited due to the inability to alter their propelled mechanisms in response to the actual physiological environment, resulting in speed and inconsistent movement directions. In this work, we designed a multi-phoretic propelled Janus nanomotor (JMSNs@Pt@P-Au) that exhibited three propelled mechanisms in response to the changes of pH value. Noteworthy is their heightened speed and remarkable tumor tissue penetration observed in vitro and in vivo without adverse effects. Such multi-phoretic propulsion offers considerable promise for developing advanced nanomachines with a stimuli-responsive switch of propulsion modes in biomedical applications., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Alzheimer's disease (AD) is a chronic neurodegenerative disease that threatens to the health of global elderly population. Acetylcholinesterase (AChE) inhibitors are an effective therapeutic agent for AD, and screening of these substances is important for AD treatment. In this work, a Pd-Pt MXene nanoenzyme was successfully synthesized by using the in-situ reduction technique. A colorimetric method for sensitive AChE inhibitor detection was designed based on enzymatic cascade reaction between Pd-Pt MXene and AChE. Briefly, The Pd-Pt MXene material exhibited excellent peroxidase (POD)-like activity due to its bimetallic composition, effectively catalyzing the oxidation of colorless 3,3,5,5-tetramethylbenzidine (TMB) to generate blue oxidized TMB (oxTMB). Under the presence of AChE and acetylthiocholine chloride (ATCh), the POD-like activity of Pd-Pt MXene was significantly inhibited. The activity of this nanoenzyme could be restored after the addition of AChE inhibitors. Using donepezil as an example, colorimetric detection was conducted within a linear range of 0.1 nmol/L to 10 nmol/L and the lowest detection boundary was only 0.35 nmol/L (S/N = 3). Finally, a paper-based platform was designed and constructed, and it has been successfully employed for AChE inhibitor detection in real samples with the aid of a smartphone. In all, this work paves a new way for designing nanoenzyme-based devices towards medicine determination or screening like AChE inhibitor., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The present article introduced an natural enzyme-covered/amino-modified Pd-Pt bimetallic-doped zeolitic imidazolate framework (NAPPZ) for ultrasensitive and specific detection of glucose. The dodecahedral nanomaterial zeolitic imidazolate framework (ZIF-8)-loaded Pd-Pt bimetallic nanoparticles endowed the composite with peroxidase-like activity. The modification with glucose oxidase (GOx) facilitated the rapid access of H 2 O 2 produced through glucose oxidation to the Pd-Pt nanoparticles vicinity reducing diffusion. GOx specifically catalyzes the transformation of glucose into H 2 O 2 , which then H 2 O 2 rapidly migrates to the Pd-Pt nanoparticles, catalyzing the oxidation of colorless o-phenylenediamine into the orange-yellow product 2,3-diaminophenazine. Based on the aforementioned cascade reaction, the NAPPZ and NAPPZ based on ChOx were utilized for detecting glucose in human urine samples and cholesterol in milk, respectively. The NAPPZ strategy presented a broad detection range (20-1100 μmol L -1 ) and a low detection limit (15.9 μmol L -1 ) for glucose, and the NAPPZ based on ChOx strategy approach offered a broad detection range (10-500 μmol L -1 ) and low detection limit (6.4 μmol L -1 ) for cholesterol. Therefore, this novel method holds significant potential in the areas of clinical diagnostics and food safety., Competing Interests: Declarations. Conflict of interest: The authors declare that they have no conflict of interest., (© 2024. The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry.)

Photodynamic therapy (PDT) eliminates cancer cells by converting endogenous oxygen into reactive oxygen species (ROS). However, its efficacy is significantly hindered by hypoxia in solid tumors. Hence, to engineer filamentous fd phage, a human-friendly bacteria-specific virus is proposed, into a nanozyme-nucleating photosensitizer-loaded tumor-homing nanofiber for enhanced production of ROS in a hypoxic tumor. Specifically, Pt-binding and tumor-homing peptides are genetically displayed on the sidewall and tip of the fd phage, respectively. The Pt-binding peptides induced nucleation and orientation of Pt nanozymes (PtNEs) on the sidewall of the phage. The resultant PtNE-coated tumor-homing phage exhibits significantly enhanced sustained catalytic conversion of hydrogen peroxide in hypoxic tumors into O 2 for producing ROS needed for PDT, compared to non-phage-templated PtNE. Density functional theory (DFT) calculations verify the catalytic mechanism of the phage-templated PtNE. After intravenous injection of the PtNE-coated indocyanine green (ICG)-loaded tumor-homing phages into breast tumor-bearing mice, the nanofibers home to the tumors and effectively inhibit tumor growth by the PtNE-enhanced PDT. The nanofibers can also serve as a tumor-homing imaging probe due to the fluorescence of ICG. This work demonstrates that filamentous phage, engineered to become tumor-homing nanozyme-nucleating tumor-hypoxia-relieving nanofibers, can act as cancer-targeting nanozymes with improved catalytic performance for effective targeted PDT., (© 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.)

The histamine (HA) in tumors plays critical roles in promoting metastasis. Herein, a series of cetirizine (CTZ) platinum(IV) complexes with antihistamine properties were developed as antimetastatic agents. Dual CTZ platinum(IV) complex with cisplatin core was screened out as a candidate displaying potent antiproliferative activities. More importantly, it exerted promising antimetastatic properties both in vitro and in vivo. Investigation of the mechanism revealed that serious DNA damage was induced, which further led to the upregulation of histone H2AX (γ-H2AX) and P53. The mitochondria-mediated apoptosis was ignited through the B-cell lymphoma-2 (Bcl-2)/Bcl-2 associated X protein (Bax)/caspase3 pathway. Moreover, the HA-histamine receptor H1 (HRH1) axis was inhibited, then the key signaling phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) was suppressed. Subsequently, the angiogenesis in tumors was restrained by suppressing the inflammatory and hypoxic microenvironment. Then, the antitumor immunity was reinforced by increasing the CD3 + and CD8 + T cells and promoting the polarization of macrophages from M2- to M1-type, which was associated with the blockade of programmed cell death ligand-1 (PD-L1) expression in tumors., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Respiratory tract fistulas, including tracheal and bronchial fistulas, usually cause prolonged hospitalization with developed complications and even death, while respiratory tract fistula healing remains challenging. Exploring effectiveness and mechanism in animal systems using well-designed bio-nanomaterials will improve our understanding of fistula management. Hyaluronate (hyaluronan or hyaluronic acid) has been widely studied as a promising coating material for bio-nanomaterials in treatment applications. Herein, by combining the intrinsic bioactivities of sodium hyaluronate (SHA) and the enzyme-like activities of platinum (Pt) nanoparticles (NPs), obtained SHA-PtNPs defined as nanozymes (Enzyme-like nanomaterials) have been proposed to treat tracheal fistulas. Results reveal that introducing SHA endows the fabrication of PtNPs with dispersibility, small particle size (3.7 nm), stability, etc. On the other hand, SHA-PtNPs present high catalase-like (3320 U/g), superoxide dismutase-like activities (129,000 U/g), and hydroxyl radicals elimination capacity, thereby exerting excellent reactive oxide species scavenging ability. We have systematically verified the above properties of SHA-PtNPs in vitro. SHA-PtNPs show outstanding biocompatibility, promote cell proliferation and migration, and have considerable hemocompatibility and hemostasis. Afterward, rabbit tracheal fistula models that were treated with SHA-PtNPs in vivo showed a significant improvement in the closure of the fistulas and an increase in quality. This was evident through a substantial decrease in inflammation, increased angiogenesis, stimulation of re-epithelialization, and highly ordered alignment of collagen fibers. No significant side effects were observed. In summary, this work initiates an in vivo treatment for tracheal fistula models by taking advantage of both naturally sourced polysaccharides and nanozymes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Designing hybrid-based drugs is one promising strategy for developing effective anticancer drugs that explore combination therapy to enhance treatment efficacy, overcome the development of drug resistance, and lower treatment duration. Bisphosphonates and Vitamin D are commonly administered drugs for the treatment of bone diseases and the prevention of bone metastases. Platinum-based and methotrexate are widely used anticancer drugs in clinics. However, their use is hampered by adverse side effects. Hybrid-based compounds containing either bisphosphonate, vitamin D, platinum-based, or methotrexate were synthesized and characterized using FTIR, 1 H-, 31 P, 13 C-NMR, and UHPLC-HRMS which confirmed their successful synthesis. The hydroxyapatite bone binding assay revealed a promising percentage binding affinity of the bisphosphonate hybrid compounds. In vitro cytotoxicity assays on MCF-7 and HT-29 cell lines revealed a promising cytotoxic effect of hybrid 19 at 50 and 100 μg/mL on HT-29 and hybrid 15 on MCF-7 at 100 μg/mL. Molecular docking and dynamics simulation analysis revealed a binding affinity of -9.70 kcal/mol for hybrid 15 against Human 3 alpha-hydroxysteroid dehydrogenase type 3, showing its capability to inhibit Human 3 alpha-hydroxysteroid dehydrogenase type 3. The Swiss ADME, ProTox-II, GUSAR (General Unrestricted Structure-Activity Relationships), and molecular docking and dynamics studies revealed that these compounds are promising anticancer compounds., (© 2024 The Author(s). Chemistry & Biodiversity published by Wiley-VHCA AG.)

This article delves into the impact of nanoparticle shape on CO chemisorption and the reactivity of Pt 13 nanocatalysts supported on reduced TiO 2 (110). Distinct reactivity in carbon monoxide adsorption is observed among nanoparticles, all composed of 13 platinum atoms but varying in shape. The calculated formation and CO adsorption energies are correlated to the electronic properties of the system and the oxidation states of the Pt atoms involved. Through an analysis of band shifting during the deposition of Pt clusters onto the oxide, and a comparison of the valence band maximum with the measured oxidation potential for the CO to CO 2 reaction, we make predictions about the system's oxidation capability in this reaction. Our findings suggest that Pt 13 clusters with cuboid, double triangle DT2 and octahedral Oh shapes, supported on the surface, are particularly advantageous for catalyzing the conversion of CO to CO 2 . Within these geometries, several configurations for CO adsorption are evaluated, focusing on the ratio between anionic and cationic Pt sites. This ratio appears to govern the activity for CO oxidation, aligning with recent experimental reports., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

In this work, a novel Electrochemiluminescence Immunosensor was constructed using PCN-224-Mn and gold-platinum nanoflowers (AuPt NFs) for the ultrasensitive detection of ochratoxin A (OTA). PCN-224 modified with Mn (II) was synthesized as a probe material. The interaction efficiency of PCN-224 with S 2 O 8 2- was also greatly improved. AuPt NFs were used as the substrate material for the electrodes. It has favorable biocompatibility, large specific surface area and can bind more antigen. Also greatly increased the electroactive surface area and conductivity of the electrode. OTA was detected using a competitive immunoassay strategy, in which OTA in the sample competes with the encapsulated antigen for a finite number of antibodies. ECLIA for the detection of OTA was designed to be highly sensitive, with a linear range from 0.0002 ng mL -1 to 1000 ng mL -1 and a LOD as low as 0.067 pg mL -1 . In addition, it was evident from the electrochemical analyses that PCN-224-Mn had a stronger and more stable ECL signal compared to the plain PCN-224. The successful preparation of specific, sensitive and reproducible ECL immunosensors confirms the great promise for the detection of OTA or other small molecule mycotoxins., Competing Interests: Declaration of competing interest We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome. We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us. We confirm that we have given due consideration to the protection of intellectual property associated with this work and that there are no impediments to publication, including the timing of publication, with respect to intellectual property. In so doing we confirmed that we have followed the regulations of our institutions concerning intellectual property. We further confirm that any aspect of the work covered in this manuscript that has involved experimental animals has been conducted with the ethical approval of all relevant bodies and that such approvals are acknowledged within the manuscript. We understand that the Corresponding Author is the sole contact for the Editorial process. He is responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs. We confirm that we have provided a current, correct email address which is accessible by the Corresponding Author and which has been configured to accept email from lijgsd@suda.edu.cn., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Microbial contamination is an important factor threatening the safety of Chinese medicine preparations, and microfluidic detection methods have demonstrated excellent advantages in the application of rapid bacterial detection. In our study, a novel optical biosensor was developed for the rapid and sensitive detection of Salmonella in traditional Chinese medicine on a microfluidic chip. Immune gold@platinum nanocatalysts (Au@PtNCs) were utilized for specific bacterial labeling, while magnetic nano-beads (MNBs) with a novel high-gradient magnetic field were employed for the specific capture of bacteria. The immune MNBs, immune Au@PtNCs, and bacterial samples were introduced into a novel passive microfluidic micromixer for full mixing, resulting in the formation of a double-antibody sandwich structure due to antigen-antibody immune reactions. Subsequently, the mixture flowed into the reaction cell, where the MNBs- Salmonella -Au@PtNCs complex was captured by the magnetic field. After washing, hydrogen peroxide-tetramethylbenzidine substrate (H 2 O 2 -TMB) was added, reacting with the Au@PtNCs peroxidase to produce a blue reaction product. This entire process was automated using a portable device, and Salmonella concentration was analyzed via a phone application. This simple biosensor has good specificity with a detection range of 9 × 10 1 -9 × 10 5 CFU/mL and can detect Salmonella concentrations as low as 90 CFU/mL within 74 min. The average recoveries of the spiked samples ranged from 76.8% to 109.5., Competing Interests: The authors declare no conflicts of interest.

Rapid gas sensing with high sensitivity and selectivity is pivotal in advanced production, in smart living, and increasingly in medical health applications. This study presents a novel Pt@InNiO x nanoflake isoprene sensor that achieves an exceptionally low limit of detection (LOD) at 2 ppb, the lowest reported for isoprene sensors to date. Notably, it exhibits high selectivity and remarkable antihumidity capacity, thus meeting the stringent requirements for lung cancer screening. To unravel the sensing mechanism, we fabricate an operando DRIFTS-Raman cell coupled to online electrical measurements. It reveals that the ultrasensitive performance of Pt@InNiO x nanoflakes stems from the activated conjugated structure of isoprene by Pt nanoclusters and from the enhanced isoprene adsorption and electron interaction due to the nanoflake morphology. The p-n junction constructed by doping Ni maintains Fermi level equilibrium, shielding it from humidity interference. Practically, we integrate these ultrasensitive Pt@InNiO x nanoflakes into a miniaturized portable electronic device that successfully distinguishes lung cancer patients with expiratory isoprene below 40 ppb, from the healthy population with isoprene above 60 ppb, enabling an accurate diagnosis in clinics. Our work not only provides a breakthrough in low-cost, noninvasive cancer screening through breath analysis but also advances the rational design of cutting-edge gas sensing materials.

Supported Pt catalysts are often subjected to severe deactivation under the conditions of high temperature and water vapor in catalytic oxidation; thus, the superior stability and water-resistant ability of catalysts have great significance for the effective degradation of volatile organic compounds (VOCs). Herein, we constructed a Pt/CeO 2 -N catalyst with an active interfacial perimeter, in which Pt species were partially embedded in the defective CeO 2 -N support to prevent the sintering. A significant charge transfer between Pt species and ceria in the embedding structure occurred via the Pt-CeO 2 interface, which induced the formation of a Pt 4+ -O v -Ce 3+ interfacial structure. Experimental research and theoretical calculations demonstrated that the active Pt 4+ -O v -Ce 3+ interface promoted the activation and migration of lattice oxygen, thus facilitating the participation of oxygen species in toluene oxidation. Consequently, Pt/CeO 2 -N showed excellent catalytic performance for toluene degradation. In situ DRIFTS and DFT calculation proved that the Pt 4+ -Ov-Ce 3+ interfacial sites served as the intrinsic active center in the dissociation of H 2 O to generate ·OH, which contributed to the formation of benzaldehyde, thus remarkably improving the water-resistant property. This study provided a facile strategy for fabricating the interfacial embedding structure to enhance the catalytic activity and water tolerance for eliminating VOCs in practical application.

Pressure-based signal transduction is of promise in developing microfluidic immunoassays such as volumetric bar-chart chips (V-chips), but new working principles are required to further simplify the methods in point-of-care testing (POCT). Herein, we developed immunosyringe sensors and integrated them with bar-chart chips for simple prick-and-read testing of prostate specific antigen (PSA) as a model target. Disposable syringes served as the host for the construction of the sandwich-type immuno-recognition system. Platinum nanoparticles (Pt NPs) as the peroxidase-mimicking detection probe catalyzed the decomposition of H 2 O 2 to produce O 2 in the syringe cylinders, enabling the pressure-driven automatic injection of liquids from the syringes. The immuno-recognition event in the syringes was thereby converted into the quantitative autoinjection behavior of the syringes, namely, immunosyringe sensors. By simply pricking the sensors to bar-chart chips, we visually and quantitatively read the immunoassay signals as the bar-chart injection distance of liquids from the syringes in channels of the chips. The immunoassay showed a limit of detection (LOD) of 0.41 ng/mL in PSA detection with satisfactory accuracy in testing clinical serum samples. Owing to the integration with the immunosyringe sensors, this method, in comparison with conventional V-chips, works in a simpler prick-and-read manner without complex chip configurations and specialized chip operations (e.g., on-chip loading of microvolume reagents and sealing treatments). Therefore, the immunoassay shows great potential in POCT applications.

A pasting-3D microfluidic paper-based analytical device (P-3D μPAD) was developed. It enabled an efficient cascade reaction between urate oxidase (UOX) and Fe/Pt-doped carbon nanoparticles (Fe/Pt-CNPs) for visual colorimetric detection of uric acid (UA). The novel Fe/Pt-CNP nanozyme performed high peroxidase-like activity toward 3,3',5,5'-tetramethylbenzidine (TMB) and H 2 O 2 with Michaelis - Menten constants (K m ) of 0.97 and 2.30 mM, respectively. The UOX-Fe/Pt-CNP system was incorporated into P-3D μPAD: the 1st layer was UOX hydrolysis reaction under alkaline pH, and the 2nd layer was Fe/Pt-CNPs catalyzing H 2 O 2 to oxidize TMB at acidic pH. The separated two layers allowed cascade reaction under different working pH without sacrificing the activity of UOX or Fe/Pt-CNPs. The images were captured and analyzed by the camera and "Color Recognition" application using a  smartphone. The linear range of P-3D μPAD for UA was 25-1000 μM with a limit of detection of 10 μM which met the requirements for clinical applications. The high accuracy of P-3D μPAD for UA detection in invasive (blood) and noninvasive (saliva) samples has been confirmed by the biochemical analyzer. This work offers a sensitive, flexible, affordable, and disposable tool for on-site UA level monitoring and provides new insight into natural enzyme and nanozyme tandem systems for biosensing., Competing Interests: Declarations. Ethics approval: All experiments were with permission from the Ethic Committees of Shanxi Bethune Hospital (no. SBQLL-2023–010), and the approval date is March 8, 2023. All volunteers signed documents providing their informed consent, and all methods were according to the relevant guidelines and institutional regulations. Competing interest: The autho s declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Objective: Using electrochemical characterization methods of stimulation electrodes as well as accelerated stimulation examinations, a safe operating field for stimulation is investigated for particularly very large Pt-Ir macroelectrodes in a Laplace configuration., Approach: Traditional methods such as Electrochemical Impedance Spectroscopy, Cyclic Voltammetry and biphasic, charge balanced current pulses were applied on Pt-Ir macroelectrodes in phosphate buffered saline solution to investigate reversible boundaries. These experiments were adapted to approach realistic working conditions., Main Results: Investigating operational conditions close to realistic use cases have shown an anti-correlation between higher scan rates and the occurrence of irreversible reactions. In addition, at higher current pulse amplitudes (>3 mA), the voltage dropping across the phase boundary (Ema and Emc) saturated. The value of the residual voltage depends on the degree of charge balance of the biphasic pulse. Thus, we have been able to detect more dissolved platinum at high residual voltages-objected by mass spectroscopic measurement., Significance: Residual voltage plays a greater role concerning reversibility in prolonged stimulation and charge imbalance of applied biphasic current pulses. The Ema saturation is suggested as a new marker for the occurrence of irreversible reactions which needs further investigation. Current amplitudes of 1 mA for the considered single electrode configuration did not lead to a capacitive voltage saturation nor a considerable dissolution of platinum ions and is thus considered as a safe operation configuration., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Leusmann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Melanoma, the most prevalent form of skin cancer, is primarily treated with surgical intervention. However, complete tumor cell removal is challenging, and surgical wounds are prone to infection, complicating treatment and increasing costs. The successful treatment of melanoma generally requires multifunctional agents that are coordinated in tumor therapy and wound healing. In this study, we developed platinum (Pt)- and selenium (Se)-based nanozymes, Pt-Se@Chitosan (PS@CS), which exhibit synergistic antitumor and bactericidal efficacy attributed to their multienzyme activity and strong photothermal conversion efficiency. Furthermore, we engineered PS@CS hydrogels capable of inhibiting tumor regrowth postsurgery and accelerating healing of infected wounds. The PS@CS and PS@CS hydrogels presented herein incorporate characteristics including catalytic therapy, photothermal therapy, antibacterial properties, and skin damage healing, providing an innovative and comprehensive therapeutic approach for melanoma treatment.

Reactive oxygen and nitrogen species (ROS/RNS) in macrophages have a potent killing effect on pathogens that infect the host. Here, we achieved in situ , quantitative detection of the homeostasis of four primary ROS/RNS (ONOO - , H 2 O 2 , NO, and NO 2 - ) and their precursors (O 2 ˙ - , NO) in phagolysosomes of single RAW 264.7 macrophages after phagocytosis of Escherichia coli with platinum-black nanoelectrodes. Enhanced bactericidal activity of the macrophages was observed by an increase in the total amount of ROS/RNS as well as the level and proportion of ONOO - , a potent bactericidal species of RNS. Moreover, both the bactericidal process and the steady-state replenishment process were dominated by the production of RNS (NO-based), revealing differences in the enzyme kinetics of the bactericidal process.

In this study, we propose a strategy to explore the impact of the proportion of tris(2-carboxyethyl)phosphine (TCEP) and 6-mercaptohexanol (MCH) on the efficiency of oligonucleotide functionalization on PDMS microfluidic channels equipped with pairs of homemade microfabricated platinum microelectrodes. We identified an optimal concentration of these compounds that enables the effective orientation and distribution of probes, thereby facilitating subsequent target hybridization. The experiment included optimizing sample injection into microfluidic channels. We used TCEP as a reducing agent to help the DNA probes adhere to the channel electrode better. This stopped the formation of disulfide bonds during the probe immobilization step. We found the optimal TCEP/MCH mixture ratio (5 mM TCEP and 50 mM MCH), which led to a more uniform distribution and orientation of the DNA probes on the platinum electrode. These optimized conditions resulted in a more compact DNA monolayer and enhanced detection capabilities. The biosensor's performance was evaluated by the detection of the hybridization of complementary DNA sequences in the presence of equimolar Fe(CN) 6 3- /Fe(CN) 6 4- . The detection of the synthetic GP8 resistance gene is facilitated by a measurable decrease in the electron transfer rate, which is directly proportional to its concentration. Under the optimized conditions, the DNA biosensor showed excellent sensitivity (with a detection limit of 10 -17 M) and high specificity when tested against noncomplementary DNA strands.

Electron-hole recombination and exogenous local hypoxia both impede the effectiveness of thermoelectric tumor catalytic therapy. Here, a thermoelectric heterojunction (Pt-TiO 2- x /Ti 3 C 2 T x -PEG) was developed to enhance charge carrier separation and alleviate tumor hypoxia. By incorporating titanium oxide with oxygen vacancies and platinum single atoms onto Ti 3 C 2 T x MXene, we not only improve the charge separation efficiency but also prevent the recombination of positive and negative charges generated by the thermoelectric effect, leading to an increased production of reactive oxygen species (ROS). Furthermore, the Pt SAs exhibited excellent catalase-mimicking (CAT-mimicking) activity, catalyzing hydrogen peroxide to generate oxygen and alleviating the hypoxic tumor microenvironment. Titanium oxide with oxygen vacancies also serves as a sonosensitizer for sonodynamic therapy (SDT), enhancing ROS generation in collaboration with thermoelectric catalytic therapy. Moreover, the photothermal conversion efficiency of Pt-TiO 2- x /Ti 3 C 2 T x -PEG is augmented by Pt SAs with a surface plasmon resonance effect, further boosting CAT-mimicking activity and thermoelectric catalytic therapy efficacy. This tumor-specific thermoelectric heterojunction integrates thermoelectric therapy, SDT, and photothermal therapy, demonstrating excellent tumor suppression efficacy both in vitro and in vivo. Therefore, this study offers highly valuable and promising insights into utilizing photothermoelectric/ultrasound-mediated methods for cancer treatment.

A biosensor is reported for electrochemical detection of ampicillin based on Au 5 Pt and DNA cycle dual-signal amplification strategy. Firstly, Au 5 Pt is prepared by reduction of chloroauric acid and chloroplatinic acid with serine-functionalized graphene quantum dot (SGQD). The resulting Au 5 Pt shows an excellent catalytic activity because of the synergy of Au and Pt. Then, Au 5 Pt is covalently combined with hairpin DNA and thionine molecule to form a redox probe. The probe was used for construction of the ampicillin biosensor coupling with DNA cycling. In the presence of ampicillin, the DNA cycle was triggered and leads to many redox probes being carried to the biosensor. This produces a significant signal amplification by oxidation and reduction of thionine molecules in these probes. The combination of Au 5 Pt catalysis with DNA cycle achieve ultrahigh sensitivity, selectivity, and stability for the electrochemical detection of ampicillin. Differential pulse voltammetry current linearly increases with the increase of ampicillin concentration in the range 1 × 10 -18 -1 × 10 -12  M with a detection limit of 3.2 × 10 -19  M (S/N = 3). The proposed analytical method has been satisfactorily used for electrochemical detection of ampicillin in milk., Competing Interests: Declarations. Competing interests: The authors declare no competing interests., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

Platinum (Pt) nanozymes with multiple intrinsic enzyme-mimicking activities have attracted extensive attention in biomedical fields due to their high catalytic activity, ease of modification, and convenient storage. However, the Pt nanozymes synthesized by the traditional method often suffer from uncontrollable morphology and poor stability under physicochemical conditions, resulting in unsatisfactory catalytic behavior in practical applications. To optimize the catalytic ability, biological templates have been introduced recently, which can guide the deposition of platinum ions on their surface to form specific morphologies and then stabilize the resulting Pt nanozymes. Given the promising potential of biotemplated Pt nanozymes in practical applications, it is essential to conduct a systematic and comprehensive review to summarize their recent research progress. In this review, we first categorize the biological templates and discuss the mechanisms as well as characteristics of each type of biotemplate in directing the growth of Pt nanozyme. Factors that impact the growth of biotemplated Pt nanozymes are then analyzed, followed by summarizing their biomedical applications. Finally, the challenges and opportunities in this field are outlined. This review article aims to provide theoretical guidance for developing Pt nanozymes with robust functionalities in biomedical applications., (© 2024 Wiley-VCH GmbH.)

To develop an efficient photocatalytic carbon dioxide (CO 2 ) reduction aimed at mitigating CO 2 emissions and greenhouse effects, we propose a straightforward strategy involving hydrogen reduction treatment of PtCu/Ti to create the PtCu/Ti-H 2 catalyst with a distinctive interface structure. Compared with the fresh PtCu/Ti catalyst and the benchmark anatase TiO 2 , the CH 4 production of the PtCu/Ti-H 2 catalyst increased by 2 times and 81.6 times, respectively. Comprehensive characterizations confirmed the formation of Pt δ+ -Ov-Ti 3+ and Cu δ+ -Ov-Ti 3+ interface structures on the hydrogen-treated PtCu/Ti-H 2 catalyst, enhancing light absorption and the separation of photogenerated charge carriers. Further investigation into the reaction mechanism revealed that the Pt δ+ -Ov-Ti 3+ and Cu δ+ -Ov-Ti 3+ species on PtCu/Ti-H 2 serve as more favorable sites for CO 2 adsorption and activation, promoting an enhanced formaldehyde mechanism. This study not only elucidates the relation between photocatalytic CO 2 reduction activity and the PtCu/Ti interface structure but also offers a novel strategy for designing alloy/oxide-based catalysts for CO 2 photoreduction, overcoming the limitations of previous studies that focused on metal or vacancy systems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)