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Chan Yee-kwan., Thesis (M.Phil.)--Chinese University of Hong Kong, 2000., Includes bibliographical references (leaves 108-131)., s in English and Chinese., Thesis Committee --- p.i, p.ii, Acknowledgments --- p.v, General Abbreviations --- p.vii, Abbreviation for Chemicals --- p.x, Table of Contents --- p.xi, List of Figures --- p.xiv, List of Tables --- p.xv, Chapter 1. --- Literature Review, Chapter 1.1 --- Salinity as a global problem --- p.1, Chapter 1.2 --- Salinity and agriculture --- p.2, Chapter 1.3 --- Plant adaptation to salinity --- p.4, Chapter 1.3.1 --- Salt secretion --- p.6, Chapter 1.3.2 --- Ion transport --- p.8, Chapter 1.3.2.1 --- Role of H+-ATPase in salt tolerance --- p.8, Chapter 1.3.2.2 --- Potassium and sodium uptake --- p.13, Chapter 1.3.2.3 --- Sodium efflux --- p.15, Chapter 1.3.3 --- Osmotic adjustment --- p.20, Chapter 1.3.3.1 --- Accumulation of mannitol --- p.21, Chapter 1.3.3.2 --- Accumulation of proline --- p.23, Chapter 1.3.3.3 --- Accumulation of glycinebetaine --- p.23, Chapter 2. --- Materials and Methods, Chapter 2.1 --- Plant materials and growth conditions --- p.26, Chapter 2.1.1 --- Surface sterilization of Arabidopsis seeds --- p.26, Chapter 2.1.2 --- Determination of sub-lethal inhibitory doses of sodium --- p.27, Chapter 2.1.3 --- Growth conditions of Arabidopsis seeds for total RNA extraction --- p.27, Chapter 2.1.4 --- NaCl dosage tests --- p.28, Chapter 2.1.5 --- Expression kinetic tests --- p.28, Chapter 2.2 --- Isolation of total RNAs --- p.28, Chapter 2.3 --- Isolation of genes differentially expressed in NaCl concentration by RAP-PCR --- p.30, Chapter 2.3.1 --- RNA fingerprinting by RAP-PCR --- p.30, Chapter 2.3.2 --- PCR reamplificatin of RAP products --- p.31, Chapter 2.3.3 --- Cloning of differentially expressed genes --- p.33, Chapter 2.3.3.1 --- Ligation of inserts into pCR-Script vector and transformation --- p.33, Chapter 2.3.3.2 --- Ligation of inserts into pBluescript II KS (+) T-vector and transformation --- p.36, Chapter 2.3.3.3 --- Screening of recombinant plasmids --- p.37, Chapter 2.4 --- Sequencing of differentially expressed genes --- p.39, Chapter 2.4.1 --- DNA cycle sequencing --- p.39, Chapter 2.5 --- Northern blot hybridization of NaCl inducible genes --- p.40, Chapter 2.5.1 --- RNA fractionation by formaldehyde gel electrophoresis --- p.40, Chapter 2.5.2 --- Northern blotting --- p.41, Chapter 2.5.3 --- Preparation of single-stranded DIG-labeled PCR probes --- p.41, Chapter 2.5.3.1 --- Isolation of Total RNA --- p.41, Chapter 2.5.3.2 --- Primer design --- p.42, Chapter 2.5.3.3 --- PCR amplification of single-stranded DIG PCR probes --- p.43, Chapter 2.5.4 --- Hybridization --- p.45, Chapter 2.5.5 --- Stringency washes --- p.46, Chapter 2.5.6 --- Chemiluminescent detection --- p.46, Chapter 3. --- Results, Chapter 3.1 --- Determination of sub-lethal inhibitory doses of sodium --- p.48, Chapter 3.2 --- Isolation of total RNA from A. thaliana treated with sodium chloride --- p.48, Chapter 3.3 --- Isolation of genes differentially expressed in sodium concentration by RNA arbitrarily primed polymerase chain reaction RAP-PCR --- p.52, Chapter 3.3.1 --- Differential cDNA fragments identified by RAP-PCR --- p.52, Chapter 3.3.2 --- PCR reamplification of RAP products --- p.52, Chapter 3.3.3 --- Cloning of selected RAP-fragments --- p.62, Chapter 3.4 --- Nucleotide sequence analysis of selected RAP PCR clones --- p.65, Chapter 3.5 --- Expression pattern analysis of salt inducible genes by northern blot hybridization --- p.75, Chapter 3.5.1 --- Preparation of single-stranded digoxigenin (DIG)-labeled probes --- p.75, Chapter 3.5.2 --- Dosage response of NaCl inducible genes --- p.79, Chapter 3.5.3 --- Expression kinetics of NaCl inducible genes --- p.80, Chapter 4. --- Discussion, Chapter 4.1 --- Isolation of RAP-PCR targets --- p.93, Chapter 4.2 --- Expression of NaCl inducible P450 genes --- p.94, Chapter 4.2.1 --- Cytochrome P450 CYP73A5 --- p.97, Chapter 4.2.2 --- Cytochrome P450 CYP83A1 --- p.98, Chapter 4.3 --- NaCl induction gene related to post-transcriptional activities --- p.99, Chapter 4.3.1 --- Glycine-rich RNA binding protein (BAC F3F19) --- p.100, Chapter 4.3.2 --- Chloroplast signal recognition particle (54CP) --- p.103, Chapter 4.4 --- Conclusion --- p.106, References --- p.108, http://library.cuhk.edu.hk/record=b5890290, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)

Yeung Chi Ching., Thesis (M.Phil.)--Chinese University of Hong Kong, 1999., Includes bibliographical references (leaves 123-134)., s in English and Chinese., p.i, Acknowledges --- p.iv, Table of contents --- p.v, List of Tables --- p.vii, List of Figures --- p.ix, Chapter Chapter 1: --- Introduction --- p.1, Chapter Chapter 2: --- Mode of reproduction of Mikania micrantha, Chapter 2.1 --- Introduction --- p.15, Chapter 2.2 --- Literature review --- p.16, Chapter 2.3 --- Materials and methods --- p.19, Chapter 2.4 --- Results --- p.23, Chapter 2.5 --- Discussions --- p.35, Chapter Chapter 3: --- Effect of sodium chloride on seed germination of Mikania micrantha, Chapter 3.1 --- Introduction --- p.42, Chapter 3.2 --- Materials and methods --- p.44, Chapter 3.3 --- Results --- p.48, Chapter 3.4 --- Discussions --- p.58, Chapter Chapter 4: --- Sodium chloride as a herbicide for Mikania Micrantha in Mai Po Marshes, Chapter 4.1 --- Introduction --- p.60, Chapter 4.2 --- Literature Review --- p.61, Chapter 4.3 --- Materials and methods --- p.74, Chapter 4.4 --- Results --- p.80, Chapter 4.5 --- Discussions --- p.113, Chapter Chapter 5: --- Conclusions --- p.118, References --- p.123, http://library.cuhk.edu.hk/record=b5889894, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)