1. Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
- Author
-
José Carlos Jiménez-López, José Fernando Florido, Juan de Dios Alché, Adoración Zafra, Lucía Palanco, Junta de Andalucía, Ministerio de Economía, Fomento y Turismo (Chile), European Commission, [Jimenez-Lopez,JC, Zafra,A, Palanco,L, Alché,JD] Plant Reproductive Biology Laboratory, Department of Biochemistry, Cell and Molecular Biology of Plants, CSIC, Granada, Spain. [Zafra,A] Elayo Group, Castillo de Locubín,Jaén, Spain. [Florido,JF] Allergy Service, Hospital Universitario San Cecilio, 18012 Granada, Spain., This study has been supported by the following grants, partially cofunded by ERDF: P2010-AGR-6274 and P2011-CVI-7487 from the Junta de Andaluc´ıa, the 201540E065 Intramural-CSIC and MINECO RETOS-COLABORACIONRTC2015-4181-2, and the EU Marie Curie (FP7-PEOPLE-2011-IOF) ref. PIOF-GA-2011-311550 awarded to Jose C.Jimenez-Lopez and Juan de Dios Alche., [Jimenez-Lopez, Jose C.] CSIC, Estn Expt Zaidin, Dept Biochem Cell & Mol Biol Plants, Plant Reprod Biol Lab, E-18008 Granada, Spain, [Zafra, Adoracion] CSIC, Estn Expt Zaidin, Dept Biochem Cell & Mol Biol Plants, Plant Reprod Biol Lab, E-18008 Granada, Spain, [Palanco, Lucia] CSIC, Estn Expt Zaidin, Dept Biochem Cell & Mol Biol Plants, Plant Reprod Biol Lab, E-18008 Granada, Spain, [de Dios Alche, Juan] CSIC, Estn Expt Zaidin, Dept Biochem Cell & Mol Biol Plants, Plant Reprod Biol Lab, E-18008 Granada, Spain, [Zafra, Adoracion] Castillo Locubin, Elayo Grp, Jaen 23670, Spain, [Fernando Florido, Jose] Hosp Univ San Cecilio, Allergy Serv, Granada 18012, Spain, ERDF from the Junta de Andalucia, Intramural-CSIC, MINECO RETOS-COLABORACION, EU, Agrifood Campus of International Excellence ceiA3, and Spanish research program Ramon y Cajal
- Subjects
0301 basic medicine ,Proteínas vegetales ,Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Molecular Conformation::Protein Conformation::Protein Structure, Tertiary [Medical Subject Headings] ,Chemicals and Drugs::Nucleic Acids, Nucleotides, and Nucleosides::Nucleic Acids::DNA::DNA, Single-Stranded::DNA, Complementary [Medical Subject Headings] ,lcsh:Medicine ,Phenomena and Processes::Chemical Phenomena::Biochemical Phenomena::Molecular Structure::Molecular Conformation::Protein Conformation::Protein Structure, Secondary [Medical Subject Headings] ,Semillas ,Storage proteins ,Epitopes ,Gene Expression Regulation, Plant ,Fiebre del heno ,Alérgenos ,Sesamum ,Proteínas de almacenamiento de semillas ,Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Pedaliaceae::Sesamum [Medical Subject Headings] ,Food science ,Vicilina ,Olivos ,Epítopos ,chemistry.chemical_classification ,Phylogenetic tree ,Pistacia ,biology ,Seed Storage Proteins ,General Medicine ,Classification ,Technology, Industry, Agriculture::Food and Beverages::Food::Nuts [Medical Subject Headings] ,Chemicals and Drugs::Biological Factors::Antigens::Epitopes [Medical Subject Headings] ,Anatomy::Plant Structures::Plant Components, Aerial::Fruit::Seeds [Medical Subject Headings] ,Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biology::Computational Biology [Medical Subject Headings] ,Olea ,Seeds ,Estructura terciaria de proteína ,Biología computacional ,ADN complementario ,Research Article ,Article Subject ,Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression::Transcription, Genetic::Transcriptome [Medical Subject Headings] ,Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Oleaceae::Olea [Medical Subject Headings] ,General Biochemistry, Genetics and Molecular Biology ,Quantitation ,03 medical and health sciences ,Phylogenetics ,Complementary DNA ,Plantas de pólenes alergénicos ,Botany ,Humans ,Storage protein ,Amino Acid Sequence ,Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Anacardiaceae::Pistacia [Medical Subject Headings] ,General Immunology and Microbiology ,lcsh:R ,Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction [Medical Subject Headings] ,Allergens ,biology.organism_classification ,Nueces ,reacción en cadena de la polimerasa ,Transcriptoma ,Filogenia ,030104 developmental biology ,chemistry ,Vicilin ,Phenomena and Processes::Genetic Phenomena::Phylogeny [Medical Subject Headings] ,Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings] ,Estructura secundaria de proteína ,Transcriptome ,Food Analysis - Abstract
Olive seeds, which are a raw material of interest, have been reported to contain 11S seed storage proteins (SSPs). However, the presence of SSPs such as 7S vicilins has not been studied. In this study, following a search in the olive seed transcriptome, 58 sequences corresponding to 7S vicilins were retrieved. A partial sequence was amplified by PCR from olive seed cDNA and subjected to phylogenetic analysis with other sequences. Structural analysis showed that olive 7S vicilin contains 9 -helixes and 22 -sheets. Additionally, 3D structural analysis displayed good superimposition with vicilin models generated from Pistacia and Sesamum. In order to assess potential allergenicity, T and B epitopes present in these proteins were identified by bioinformatic approaches. Different motifs were observed among the species, as well as some species-specific motifs. Finally, expression analysis of vicilins was carried out in protein extracts obtained from seeds of different species, including the olive. Noticeable bands were observed for all species in the 15–75 kDa MW interval, which were compatible with vicilins. The reactivity of the extracts to sera from patients allergic to nuts was also analysed. The findings with regard to the potential use of olive seed as food are discussed. [EN], This study has been supported by the following grants, partially cofunded by ERDF: P2010-AGR-6274 and P2011-CVI-7487 from the Junta de Andalucía; the 201540E065 Intramural-CSIC and MINECO RETOS-COLABORACIÓN RTC2015-4181-2; and the EU Marie Curie (FP7-PEOPLE-2011-IOF) ref. PIOF-GA-2011-311550.
- Published
- 2016