62 results on '"Plant Stress Resilience"'
Search Results
2. Impact of individual, combined and sequential stress on photosynthesis machinery in rice (Oryza sativa L)
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Sub Plant Stress Resilience, Plant Stress Resilience, Anwar, Khalid, Joshi, Rohit, Bahuguna, Rajeev N., Govindjee, Govindjee, Sasidharan, Rashmi, Singla-Pareek, Sneh L., Pareek, Ashwani, Sub Plant Stress Resilience, Plant Stress Resilience, Anwar, Khalid, Joshi, Rohit, Bahuguna, Rajeev N., Govindjee, Govindjee, Sasidharan, Rashmi, Singla-Pareek, Sneh L., and Pareek, Ashwani
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- 2024
3. Azolla cultivation enables phosphate extraction from inundated former agricultural soils
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Sub Plant Stress Resilience, Plant Stress Resilience, Vroom, R. J.E., Smolders, A. J.P., Van de Riet, B. P., Lamers, L. P.M., Güngör, E., Krosse, S., Verheggen-Kleinheerenbrink, G. M., Van der Wal, N. R., Kosten, S., Sub Plant Stress Resilience, Plant Stress Resilience, Vroom, R. J.E., Smolders, A. J.P., Van de Riet, B. P., Lamers, L. P.M., Güngör, E., Krosse, S., Verheggen-Kleinheerenbrink, G. M., Van der Wal, N. R., and Kosten, S.
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- 2024
4. The Mediator complex subunit MED25 interacts with HDA9 and PIF4 to regulate thermomorphogenesis
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Shapulatov, Umidjon, Van zanten, Martijn, Van hoogdalem, Mark, Meisenburg, Mara, Van hall, Alexander, Kappers, Iris, Fasano, Carlo, Facella, Paolo, Loh, Chi Cheng, Perrella, Giorgio, Van der krol, Alexander, Plant Stress Resilience, Molecular Plant Physiology, Sub Plant Stress Resilience, Sub Molecular Plant Physiology, Plant Stress Resilience, Molecular Plant Physiology, Sub Plant Stress Resilience, and Sub Molecular Plant Physiology
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Histone Deacetylases/genetics ,Crop Physiology ,Basic Helix-Loop-Helix Transcription Factors/genetics ,Physiology ,Transcription Factors/genetics ,Plant Science ,Plant ,Arabidopsis/metabolism ,Arabidopsis Proteins/genetics ,Phytochrome/metabolism ,Gene Expression Regulation ,Gene Expression Regulation, Plant ,Genetics ,Life Science ,Laboratorium voor Plantenfysiologie ,EPS ,Gewasfysiologie ,Mediator Complex/genetics ,Laboratory of Plant Physiology - Abstract
Thermomorphogenesis is, among other traits, characterized by enhanced hypocotyl elongation due to the induction of auxin biosynthesis genes like YUCCA8 by transcription factors, most notably PHYTOCHROME INTERACTING FACTOR 4 (PIF4). Efficient binding of PIF4 to the YUCCA8 locus under warmth depends on HISTONE DEACETYLASE 9 (HDA9) activity, which mediates histone H2A.Z depletion at the YUCCA8 locus. However, HDA9 lacks intrinsic DNA-binding capacity, and how HDA9 is recruited to YUCCA8, and possibly other PIF4-target sites, is currently not well understood. The Mediator complex functions as a bridge between transcription factors bound to specific promoter sequences and the basal transcription machinery containing RNA polymerase II. Mutants of Mediator component Mediator25 (MED25) exhibit reduced hypocotyl elongation and reduced expression of YUCCA8 at 27°C. In line with a proposed role for MED25 in thermomorphogenesis in Arabidopsis (Arabidopsis thaliana), we demonstrated an enhanced association of MED25 to the YUCCA8 locus under warmth and interaction of MED25 with both PIF4 and HDA9. Genetic analysis confirmed that MED25 and HDA9 operate in the same pathway. Intriguingly, we also showed that MED25 destabilizes HDA9 protein. Based on our findings, we propose that MED25 recruits HDA9 to the YUCCA8 locus by binding to both PIF4 and HDA9.
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- 2023
5. The root-knot nematode effector MiMSP32 targets host 12-oxophytodienoate reductase 2 to regulate plant susceptibility
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Verhoeven, Ava, Finkers-Tomczak, Anna, Prins, Pjotr, Valkenburg-van Raaij, Debbie R, van Schaik, Casper C, Overmars, Hein, van Steenbrugge, Joris Jm, Tacken, Wannes, Varossieau, Koen, Slootweg, Erik J, Kappers, Iris F, Quentin, Michaël, Goverse, Aska, Sterken, Mark G, Smant, Geert, Plant Stress Resilience, Sub Plant Stress Resilience, Plant Stress Resilience, and Sub Plant Stress Resilience
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effector ,host target ,Physiology ,positive selection ,nematode ,12-oxophytodienoic acid ,Plant Science ,jasmonates ,Meloidogyne incognita - Abstract
To establish persistent infections in host plants, herbivorous invaders, such as root-knot nematodes, must rely on effectors for suppressing damage-induced jasmonate-dependent host defenses. However, at present, the effector mechanisms targeting the biosynthesis of biologically active jasmonates to avoid adverse host responses are unknown. Using yeast two-hybrid, in planta co-immunoprecipitation, and mutant analyses, we identified 12-oxophytodienoate reductase 2 (OPR2) as an important host target of the stylet-secreted effector MiMSP32 of the root-knot nematode Meloidogyne incognita. MiMSP32 has no informative sequence similarities with other functionally annotated genes but was selected for the discovery of novel effector mechanisms based on evidence of positive, diversifying selection. OPR2 catalyzes the conversion of a derivative of 12-oxophytodienoate to jasmonic acid (JA) and operates parallel to 12-oxophytodienoate reductase 3 (OPR3), which controls the main pathway in the biosynthesis of jasmonates. We show that MiMSP32 targets OPR2 to promote parasitism of M. incognita in host plants independent of OPR3-mediated JA biosynthesis. Artificially manipulating the conversion of the 12-oxophytodienoate by OPRs increases susceptibility to multiple unrelated plant invaders. Our study is the first to shed light on a novel effector mechanism targeting this process to regulate the susceptibility of host plants.
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- 2023
6. Flooding resilience is determined by leaf age in Arabidopsis thaliana
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Plant Stress Resilience, Plant-Environment Signaling, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Sub Plant Ecophysiology, Sasidharan, Rashmi, Voesenek, Rens, Rankenberg, Thomas Hendrik, Plant Stress Resilience, Plant-Environment Signaling, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Sub Plant Ecophysiology, Sasidharan, Rashmi, Voesenek, Rens, and Rankenberg, Thomas Hendrik
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- 2023
7. The Mediator complex subunit MED25 interacts with HDA9 and PIF4 to regulate thermomorphogenesis
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Plant Stress Resilience, Molecular Plant Physiology, Sub Plant Stress Resilience, Sub Molecular Plant Physiology, Shapulatov, Umidjon, Van zanten, Martijn, Van hoogdalem, Mark, Meisenburg, Mara, Van hall, Alexander, Kappers, Iris, Fasano, Carlo, Facella, Paolo, Loh, Chi Cheng, Perrella, Giorgio, Van der krol, Alexander, Plant Stress Resilience, Molecular Plant Physiology, Sub Plant Stress Resilience, Sub Molecular Plant Physiology, Shapulatov, Umidjon, Van zanten, Martijn, Van hoogdalem, Mark, Meisenburg, Mara, Van hall, Alexander, Kappers, Iris, Fasano, Carlo, Facella, Paolo, Loh, Chi Cheng, Perrella, Giorgio, and Van der krol, Alexander
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- 2023
8. Multi-stress resilience in plants recovering from submergence
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Plant Stress Resilience, Plant-Environment Signaling, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Yuan, Li-Bing, Chen, Mo-Xian, Wang, Lin-Na, Sasidharan, Rashmi, Voesenek, Laurentius A C J, Xiao, Shi, Plant Stress Resilience, Plant-Environment Signaling, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Yuan, Li-Bing, Chen, Mo-Xian, Wang, Lin-Na, Sasidharan, Rashmi, Voesenek, Laurentius A C J, and Xiao, Shi
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- 2023
9. The root-knot nematode effector MiMSP32 targets host 12-oxophytodienoate reductase 2 to regulate plant susceptibility
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Plant Stress Resilience, Sub Plant Stress Resilience, Verhoeven, Ava, Finkers-Tomczak, Anna, Prins, Pjotr, Valkenburg-van Raaij, Debbie R, van Schaik, Casper C, Overmars, Hein, van Steenbrugge, Joris Jm, Tacken, Wannes, Varossieau, Koen, Slootweg, Erik J, Kappers, Iris F, Quentin, Michaël, Goverse, Aska, Sterken, Mark G, Smant, Geert, Plant Stress Resilience, Sub Plant Stress Resilience, Verhoeven, Ava, Finkers-Tomczak, Anna, Prins, Pjotr, Valkenburg-van Raaij, Debbie R, van Schaik, Casper C, Overmars, Hein, van Steenbrugge, Joris Jm, Tacken, Wannes, Varossieau, Koen, Slootweg, Erik J, Kappers, Iris F, Quentin, Michaël, Goverse, Aska, Sterken, Mark G, and Smant, Geert
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- 2023
10. Arabidopsis latent virus 1, a comovirus widely spread in Arabidopsis thaliana collections
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Plant Stress Resilience, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Sub Molecular Plant Physiology, Plant-Environment Signaling, Molecular Plant Physiology, Verhoeven, Ava, Kloth, Karen J., Kupczok, Anne, Oymans, Geert H., Damen, Janna, Rijnsburger, Karin, Jiang, Zhang, Deelen, Cas, Sasidharan, Rashmi, Van zanten, Martijn, Van der vlugt, René A. A., Plant Stress Resilience, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Sub Molecular Plant Physiology, Plant-Environment Signaling, Molecular Plant Physiology, Verhoeven, Ava, Kloth, Karen J., Kupczok, Anne, Oymans, Geert H., Damen, Janna, Rijnsburger, Karin, Jiang, Zhang, Deelen, Cas, Sasidharan, Rashmi, Van zanten, Martijn, and Van der vlugt, René A. A.
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- 2023
11. A conserved signaling axis integrates conicting environmental drought and heat signals to control stomatal aperture in plants
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Sub Plant Stress Resilience, Plant Stress Resilience, De Smet, Ive, Praat, Myrthe, Pizzio, Gaston, Jiang, Zhang, Driever, Steven, Wang, Ren, Leonhardt, Nathalie, Kinoshita, Toshinori, Vanneste, Steffen, Rodriguez, Pedro, Van Zanten, Martijn, Sub Plant Stress Resilience, Plant Stress Resilience, De Smet, Ive, Praat, Myrthe, Pizzio, Gaston, Jiang, Zhang, Driever, Steven, Wang, Ren, Leonhardt, Nathalie, Kinoshita, Toshinori, Vanneste, Steffen, Rodriguez, Pedro, and Van Zanten, Martijn
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- 2023
12. Crane fly semiochemical overrules plant control over cyanobiont in Azolla symbioses
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Sub Plant Stress Resilience, Sub Molecular Plant Physiology, Molecular Plant Physiology, Plant Stress Resilience, Güngör, Erbil, Savary, Jérôme, Adema, Kelvin, Dijkhuizen, Laura W., Keilwagen, Jens, Himmelbach, Axel, Mascher, Martin, Koppers, Nils, Bräutigam, Andrea, Hove, Charles van, Riant, Olivier, Nierzwicki-Bauer, Sandra, Schluepmann, Henriette, Sub Plant Stress Resilience, Sub Molecular Plant Physiology, Molecular Plant Physiology, Plant Stress Resilience, Güngör, Erbil, Savary, Jérôme, Adema, Kelvin, Dijkhuizen, Laura W., Keilwagen, Jens, Himmelbach, Axel, Mascher, Martin, Koppers, Nils, Bräutigam, Andrea, Hove, Charles van, Riant, Olivier, Nierzwicki-Bauer, Sandra, and Schluepmann, Henriette
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- 2023
13. Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES
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Sub Plant Stress Resilience, Plant Stress Resilience, Yang, Huanjie, Kim, Xeniya, Skłenar, Jan, Aubourg, Sébastien, Sancho-Andrés, Gloria, Stahl, Elia, Guillou, Marie-Charlotte, Gigli-Bisceglia, Nora, Canh, Loup Tran Van, Bender, Kyle W., Stintzi, Annick, Reymond, Philippe, Sánchez-Rodríguez, Clara, Testerink, Christa, Renou, Jean-Pierre, Menke, Frank L. H., Schaller, Andreas, Rhodes, Jack, Zipfel, Cyril, Sub Plant Stress Resilience, Plant Stress Resilience, Yang, Huanjie, Kim, Xeniya, Skłenar, Jan, Aubourg, Sébastien, Sancho-Andrés, Gloria, Stahl, Elia, Guillou, Marie-Charlotte, Gigli-Bisceglia, Nora, Canh, Loup Tran Van, Bender, Kyle W., Stintzi, Annick, Reymond, Philippe, Sánchez-Rodríguez, Clara, Testerink, Christa, Renou, Jean-Pierre, Menke, Frank L. H., Schaller, Andreas, Rhodes, Jack, and Zipfel, Cyril
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- 2023
14. Is hypoxia good or bad? Role of oxygen levels in maize kernel development
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Plant Stress Resilience, Sub Plant Stress Resilience, Sanclemente, Maria Angelica, Plant Stress Resilience, Sub Plant Stress Resilience, and Sanclemente, Maria Angelica
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- 2023
15. The genetic basis of plants’ battle against witchweeds: linking immune responses to distinct resistance mechanisms
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Plant-Environment Signaling, Sub Plant-Environment Signaling, Sub Plant Stress Resilience, Plant Stress Resilience, Jhu, Min-Yao, Kawa, Dorota, Brady, Siobhán M, Plant-Environment Signaling, Sub Plant-Environment Signaling, Sub Plant Stress Resilience, Plant Stress Resilience, Jhu, Min-Yao, Kawa, Dorota, and Brady, Siobhán M
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- 2023
16. A helping hand when drowning: The versatile role of ethylene in root flooding resilience
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Sub Plant Stress Resilience, Plant Stress Resilience, Leeggangers, Hendrika A.C.F., Rodriguez Granados, Natalia Y., Macias Honti, Monika G., Sasidharan, Rashmi, Sub Plant Stress Resilience, Plant Stress Resilience, Leeggangers, Hendrika A.C.F., Rodriguez Granados, Natalia Y., Macias Honti, Monika G., and Sasidharan, Rashmi
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- 2023
17. Plant responses to limited aeration: Advances and future challenges
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Plant Stress Resilience, Sub Plant Stress Resilience, Dalle Carbonare, Laura, Jiménez, Juan de la Cruz, Lichtenauer, Sophie, van Veen, Hans, Plant Stress Resilience, Sub Plant Stress Resilience, Dalle Carbonare, Laura, Jiménez, Juan de la Cruz, Lichtenauer, Sophie, and van Veen, Hans
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- 2023
18. Plasticity of maternal environment-dependent expression-QTLs of tomato seeds
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Plant Stress Resilience, Theoretical Biology and Bioinformatics, Sub Plant Stress Resilience, Sub Bioinformatics, Sterken, Mark G, Nijveen, Harm, van Zanten, Martijn, Jiménez-Gómez, Jose M, Geshnizjani, Nafiseh, Willems, Leo A J, Rienstra, Juriaan, Hilhorst, Henk W M, Ligterink, Wilco, Snoek, Basten L, Plant Stress Resilience, Theoretical Biology and Bioinformatics, Sub Plant Stress Resilience, Sub Bioinformatics, Sterken, Mark G, Nijveen, Harm, van Zanten, Martijn, Jiménez-Gómez, Jose M, Geshnizjani, Nafiseh, Willems, Leo A J, Rienstra, Juriaan, Hilhorst, Henk W M, Ligterink, Wilco, and Snoek, Basten L
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- 2023
19. 25 Years of thermomorphogenesis research: milestones and perspectives
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Sub Plant Stress Resilience, Plant Stress Resilience, Quint, Marcel, Delker, Carolin, Balasubramanian, Sureshkumar, Balcerowicz, Martin, Casal, Jorge J, Castroverde, Christian Danve M, Chen, Meng, Chen, Xuemei, De Smet, Ive, Fankhauser, Christian, Franklin, Keara A, Halliday, Karen J, Hayes, Scott, Jiang, Danhua, Jung, Jae-Hoon, Kaiserli, Eirini, Kumar, S Vinod, Maag, Daniel, Oh, Eunkyoo, Park, Chung-Mo, Penfield, Steven, Perrella, Giorgio, Prat, Salomé, Reis, Rodrigo S, Wigge, Philip A, Willige, Björn C, van Zanten, Martijn, Sub Plant Stress Resilience, Plant Stress Resilience, Quint, Marcel, Delker, Carolin, Balasubramanian, Sureshkumar, Balcerowicz, Martin, Casal, Jorge J, Castroverde, Christian Danve M, Chen, Meng, Chen, Xuemei, De Smet, Ive, Fankhauser, Christian, Franklin, Keara A, Halliday, Karen J, Hayes, Scott, Jiang, Danhua, Jung, Jae-Hoon, Kaiserli, Eirini, Kumar, S Vinod, Maag, Daniel, Oh, Eunkyoo, Park, Chung-Mo, Penfield, Steven, Perrella, Giorgio, Prat, Salomé, Reis, Rodrigo S, Wigge, Philip A, Willige, Björn C, and van Zanten, Martijn
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- 2023
20. Parallels between drought and flooding: An integrated framework for plant eco-physiological responses to water stress
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Environmental Sciences, Sub Theoretical Biology, Sub Plant Stress Resilience, Global Ecohydrology and Sustainability, Theoretical Biology and Bioinformatics, Plant Stress Resilience, Chen, Siluo, ten Tusscher, Kirsten H. W. J., Sasidharan, Rashmi, Dekker, Stefan C., de Boer, Hugo J., Environmental Sciences, Sub Theoretical Biology, Sub Plant Stress Resilience, Global Ecohydrology and Sustainability, Theoretical Biology and Bioinformatics, Plant Stress Resilience, Chen, Siluo, ten Tusscher, Kirsten H. W. J., Sasidharan, Rashmi, Dekker, Stefan C., and de Boer, Hugo J.
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- 2023
21. Arabidopsis latent virus 1, a comovirus widely spread in Arabidopsis thaliana collections
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Verhoeven, Ava, Kloth, Karen J., Kupczok, Anne, Oymans, Geert H., Damen, Janna, Rijnsburger, Karin, Jiang, Zhang, Deelen, Cas, Sasidharan, Rashmi, Van zanten, Martijn, Van der vlugt, René A. A., Plant Stress Resilience, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Sub Molecular Plant Physiology, Plant-Environment Signaling, Molecular Plant Physiology, Plant Stress Resilience, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Sub Molecular Plant Physiology, Plant-Environment Signaling, and Molecular Plant Physiology
- Subjects
drought resilience ,Arabidopsis thaliana ,Bioinformatics ,Physiology ,Laboratory of Virology ,comovirus ,OT Team Fruit-Bomen ,RNA sequencing ,Plant Science ,PE&RC ,Laboratorium voor Entomologie ,Arabidopsis latent virus 1 (ArLV1) ,Laboratorium voor Virologie ,Biointeractions and Plant Health ,sequence read archives ,Bioinformatica ,EPS ,Laboratory of Entomology ,Laboratory of Nematology ,Laboratorium voor Nematologie - Abstract
SummaryTranscriptome studies of Illumina RNA-seq datasets of different Arabidopsis thaliana natural accessions and T-DNA mutants revealed the presence of two virus-like RNA sequences which showed the typical two segmented genome characteristics of a comovirus.This comovirus did not induce any visible symptoms in infected Arabidopsis plants cultivated under standard laboratory conditions. Hence it was named Arabidopsis latent virus 1 (ArLV1). Virus infectivity in Arabidopsis plants was confirmed by RT-qPCR, transmission electron microscopy and mechanical inoculation. ArLV1 can also mechanically infect Nicotiana benthamiana, causing distinct mosaic symptoms.A bioinformatics investigation of Arabidopsis RNA-Seq repositories, including nearly 6500 Sequence Read Archives (SRAs) in the NCBI SRA database, revealed the presence of ArLV1 in 25% of all archived natural Arabidopsis accessions and in 8.5% of all analyzed SRAs. ArLV1 could also be detected in Arabidopsis plants collected from the wild.ArLV1 is highly seed-transmissible with up to 40% incidence on the progeny derived from infected Arabidopsis plants. This has likely led to a worldwide distribution in the model plant Arabidopsis with yet unknown effects on plant performance in a substantial number of studies.Plain language summaryWe identified Arabidopsis latent virus 1 (ArLV1), a comovirus that infects the model plant Arabidopsis thaliana without causing any visible symptoms. It is efficiently spread by transmission via seeds to the plant progeny. ArLV1 is infectious to Arabidopsis plants and another model plant, Nicotiana benthamiana. By analyzing public sequencing data, we found that ArLV1 is widely spread in Arabidopsis laboratory collections worldwide. Moreover, it was also detected in wild Arabidopsis plants collected from different locations in the Netherlands and Spain, suggesting that it is a virus that naturally occurs in Arabidopsis.
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- 2023
22. Wandering between hot and cold: temperature dose-dependent responses
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Zhu, Tingting, van Zanten, Martijn, De Smet, Ive, Sub Plant Stress Resilience, Plant Stress Resilience, Sub Plant Stress Resilience, and Plant Stress Resilience
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Thermotolerance ,Freezing tolerance ,Hot Temperature ,Light ,Arabidopsis Proteins ,Acclimatization ,Arabidopsis ,Temperature ,Biodiversity ,Plant Science ,Cell elongation ,Hormone ,Cold Temperature ,Root-growth ,Phytochromes ,Taverne ,Perception ,Arabidopsis/genetics ,Acclimatization/physiology ,Acclimation - Abstract
Plants in most natural habitats are exposed to a continuously changing environment, including fluctuating temperatures. Temperature variations can trigger acclimation or tolerance responses, depending on the severity of the signal. To guarantee food security under a changing climate, we need to fully understand how temperature response and tolerance are triggered and regulated. Here, we put forward the concept that responsiveness to temperature should be viewed in the context of dose-dependency. We discuss physiological, developmental, and molecular examples, predominantly from the model plant Arabidopsis thaliana, illustrating monophasic signaling responses across the physiological temperature gradient.
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- 2022
23. Ethylene - the lifeguard hormone
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Plant Stress Resilience, Sub Plant Stress Resilience, Sasidharan, Rashmi, Plant Stress Resilience, Sub Plant Stress Resilience, and Sasidharan, Rashmi
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- 2022
24. Ethylene-mediated phosphorylation of ORESARA1 induces sequential leaf death during flooding in Arabidopsis
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Plant Stress Resilience, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Plant-Environment Signaling, Rankenberg, Tom, Veen, Hans van, Sedaghatmehr, Mastoureh, Liao, Che-Yang, Devaiah, Muthanna Biddanda, Balazadeh, Salma, Sasidharan, Rashmi, Plant Stress Resilience, Sub Plant Stress Resilience, Sub Plant-Environment Signaling, Plant-Environment Signaling, Rankenberg, Tom, Veen, Hans van, Sedaghatmehr, Mastoureh, Liao, Che-Yang, Devaiah, Muthanna Biddanda, Balazadeh, Salma, and Sasidharan, Rashmi
- Published
- 2022
25. Wandering between hot and cold: temperature dose-dependent responses
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Sub Plant Stress Resilience, Plant Stress Resilience, Zhu, Tingting, van Zanten, Martijn, De Smet, Ive, Sub Plant Stress Resilience, Plant Stress Resilience, Zhu, Tingting, van Zanten, Martijn, and De Smet, Ive
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- 2022
26. Ethylene — the lifeguard hormone
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Sasidharan, Rashmi, Sub Plant Stress Resilience, Plant Stress Resilience, Plant Stress Resilience, and Sub Plant Stress Resilience
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Taverne ,Cell Biology ,Ethylenes ,Molecular Biology ,Hormones - Published
- 2022
27. Effects of sub-lethal single, simultaneous, and sequential abiotic stresses on phenotypic traits of Arabidopsis thaliana
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Sub Plant Ecophysiology, Global Ecohydrology and Sustainability, Sub Molecular Plant Physiology, Sub Plant-Environment Signaling, Sub Developmental Biology, Sub Plant Stress Resilience, Plant Ecophysiology, Plant Stress Resilience, Plant-Environment Signaling, Molecular Plant Physiology, Developmental Biology, Morales, A., Boer, H. J. de, Douma, J. C., Elsen, S, Engels, S., Glimmerveen, T., Sajeev, N., Huber, M., Luimes, M., Luitjens, E., Raatjes, K., Hsieh, C., Teapal, J., Wildenbeest, T., Jiang, Z., Pareek, A., Singla-Pareek, S. L., Yin, X., Evers, J.B., Anten, N.P.R., Zanten, M. van, Sasidharan, R., Sub Plant Ecophysiology, Global Ecohydrology and Sustainability, Sub Molecular Plant Physiology, Sub Plant-Environment Signaling, Sub Developmental Biology, Sub Plant Stress Resilience, Plant Ecophysiology, Plant Stress Resilience, Plant-Environment Signaling, Molecular Plant Physiology, Developmental Biology, Morales, A., Boer, H. J. de, Douma, J. C., Elsen, S, Engels, S., Glimmerveen, T., Sajeev, N., Huber, M., Luimes, M., Luitjens, E., Raatjes, K., Hsieh, C., Teapal, J., Wildenbeest, T., Jiang, Z., Pareek, A., Singla-Pareek, S. L., Yin, X., Evers, J.B., Anten, N.P.R., Zanten, M. van, and Sasidharan, R.
- Published
- 2021
28. Multi-stress resilience in plants recovering from submergence
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Yuan, Li-Bing, Chen, Mo-Xian, Wang, Lin-Na, Sasidharan, Rashmi, Voesenek, Laurentius A C J, Xiao, Shi, Plant Stress Resilience, Plant-Environment Signaling, Sub Plant Stress Resilience, and Sub Plant-Environment Signaling
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flooding ,Plant Science ,metabolic homeostasis ,Agronomy and Crop Science ,multi-stress resilience ,post-submergence recovery ,regulatory network ,Biotechnology - Abstract
Submergence limits plants' access to oxygen and light, causing massive changes in metabolism; after submergence, plants experience additional stresses, including reoxygenation, dehydration, photoinhibition and accelerated senescence. Plant responses to waterlogging and partial or complete submergence have been well studied, but our understanding of plant responses during post-submergence recovery remains limited. During post-submergence recovery, whether a plant can repair the damage caused by submergence and reoxygenation and re-activate key processes to continue to grow, determines whether the plant survives. Here, we summarize the challenges plants face when recovering from submergence, primarily focusing on studies of Arabidopsis thaliana and rice (Oryza sativa). We also highlight recent progress in elucidating the interplay among various regulatory pathways, compare post-hypoxia reoxygenation between plants and animals and provide new perspectives for future studies.
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- 2023
29. Geography, altitude, agriculture and hypoxia.
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Holdsworth MJ, Liu H, Castellana S, Abbas M, Liu J, and Perata P
- Abstract
Reduced oxygen availability (hypoxia) represents a key plant abiotic stress in natural and agricultural systems, but conversely it is also an important component of normal growth and development. We review recent advances that demonstrate how genetic adaptations associated with hypoxia impact the known plant oxygen sensing mechanism through the PLANT CYSTEINE OXIDASE (PCO) N-degron pathway. Only three protein substrates of this pathway have been identified, and all adaptations identified to date are associated with the most important of these, the group VII ETHYLENE RESPONSE FACTOR transcription factors (ERFVIIs). We discuss how geography, altitude, and agriculture have all shaped molecular responses to hypoxia, and how these responses have emerged at different taxonomic levels through the evolution of land plants. Understanding how ecological and agricultural genetic variation acts positively to enhance hypoxia tolerance will provide novel tools and concepts to improve the performance of crops in the face of increasing extreme flooding events., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
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- 2024
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30. ERFVII-controlled hypoxia responses are in part facilitated by MEDIATOR SUBUNIT 25 in Arabidopsis thaliana.
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Schippers JHM, von Bongartz K, Laritzki L, Frohn S, Frings S, Renziehausen T, Augstein F, Winkels K, Sprangers K, Sasidharan R, Vertommen D, Van Breusegem F, Hartman S, Beemster GTS, Mhamdi A, van Dongen JT, and Schmidt-Schippers RR
- Abstract
Flooding impairs plant growth through oxygen deprivation, which activates plant survival and acclimation responses. Transcriptional responses to low oxygen are generally associated with the activation of group VII ETHYLENE-RESPONSE FACTOR (ERFVII) transcription factors. However, the exact mechanisms and molecular components by which ERFVII factors initiate gene expression are not fully elucidated. Here, we show that the ERFVII factors RELATED TO APETALA 2.2 (RAP2.2) and RAP2.12 cooperate with the Mediator complex subunit AtMED25 to coordinate gene expression under hypoxia in Arabidopsis thaliana. Respective med25 knock-out mutants display reduced low-oxygen stress tolerance. AtMED25 physically associates with a distinct set of hypoxia core genes and its loss partially impairs transcription under hypoxia due to decreased RNA polymerase II recruitment. Association of AtMED25 with target genes requires the presence of ERFVII transcription factors. Next to ERFVII protein stabilisation, also the composition of the Mediator complex including AtMED25 is potentially affected by hypoxia stress as shown by protein-complex pulldown assays. The dynamic response of the Mediator complex to hypoxia is furthermore supported by the fact that two subunits, AtMED8 and AtMED16, are not involved in the establishment of hypoxia tolerance, whilst both act in coordination with AtMED25 under other environmental conditions. We furthermore show that AtMED25 function under hypoxia is independent of ethylene signalling. Finally, functional conservation at the molecular level was found for the MED25-ERFVII module between A. thaliana and the monocot species Oryza sativa, pointing to a potentially universal role of MED25 in coordinating ERFVII-dependent transcript responses to hypoxia in plants., (© 2024 The Author(s). The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
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- 2024
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31. Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum.
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Taylor T, Daksa J, Shimels MZ, Etalo DW, Thiombiano B, Walmsey A, Chen AJ, Bouwmeester HJ, Raaijmakers JM, Brady SM, and Kawa D
- Abstract
The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds. Key features • This protocol provides a detailed description of the methods used in Kawa et al. [1]. • This protocol is optimized to assess soil suppressiveness to Striga infection by using natural field-collected soil and the same soil sterilized by gamma-radiation. • This protocol is optimized to test bacterial (and not fungal) isolates. • This protocol can be easily extended to other host-parasite-microbiome systems., Competing Interests: Competing interestsThere are no conflicts of interest or competing interests., (©Copyright : © 2024 The Authors; This is an open access article under the CC BY license.)
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- 2024
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32. Arabinosylation of cell wall extensin is required for the directional response to salinity in roots.
- Author
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Zou Y, Gigli-Bisceglia N, van Zelm E, Kokkinopoulou P, Julkowska MM, Besten M, Nguyen TP, Li H, Lamers J, de Zeeuw T, Dongus JA, Zeng Y, Cheng Y, Koevoets IT, Jørgensen B, Giesbers M, Vroom J, Ketelaar T, Petersen BL, Engelsdorf T, Sprakel J, Zhang Y, and Testerink C
- Subjects
- Glycoproteins metabolism, Glycoproteins genetics, Plant Proteins metabolism, Plant Proteins genetics, Gravitropism, Arabinose metabolism, Sodium Chloride pharmacology, Gene Expression Regulation, Plant drug effects, Glycosylation, Cell Wall metabolism, Plant Roots metabolism, Plant Roots growth & development, Plant Roots genetics, Plant Roots drug effects, Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis drug effects, Arabidopsis Proteins metabolism, Arabidopsis Proteins genetics, Salinity
- Abstract
Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
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- 2024
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33. Far-red light enrichment affects gene expression and architecture as well as growth and photosynthesis in rice.
- Author
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Huber M, de Boer HJ, Romanowski A, van Veen H, Buti S, Kahlon PS, van der Meijden J, Koch J, and Pierik R
- Subjects
- Plant Leaves radiation effects, Plant Leaves growth & development, Plant Leaves genetics, Plant Leaves physiology, Carbon Dioxide metabolism, Photoperiod, Biomass, Transcriptome, Red Light, Oryza genetics, Oryza growth & development, Oryza radiation effects, Oryza physiology, Photosynthesis radiation effects, Light, Gene Expression Regulation, Plant radiation effects, Plant Shoots growth & development, Plant Shoots radiation effects, Plant Shoots genetics
- Abstract
Plants use light as a resource and signal. Photons within the 400-700 nm waveband are considered photosynthetically active. Far-red photons (FR, 700-800 nm) are used by plants to detect nearby vegetation and elicit the shade avoidance syndrome. In addition, FR photons have also been shown to contribute to photosynthesis, but knowledge about these dual effects remains scarce. Here, we study shoot-architectural and photosynthetic responses to supplemental FR light during the photoperiod in several rice varieties. We observed that FR enrichment only mildly affected the rice transcriptome and shoot architecture as compared to established model species, whereas leaf formation, tillering and biomass accumulation were clearly promoted. Consistent with this growth promotion, we found that CO
2 -fixation in supplemental FR was strongly enhanced, especially in plants acclimated to FR-enriched conditions as compared to control conditions. This growth promotion dominates the effects of FR photons on shoot development and architecture. When substituting FR enrichment with an end-of-day FR pulse, this prevented photosynthesis-promoting effects and elicited shade avoidance responses. We conclude that FR photons can have a dual role, where effects depend on the environmental context: in addition to being an environmental signal, they are also a potent source of harvestable energy., (© 2024 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.)- Published
- 2024
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34. Phylotranscriptomics provides a treasure trove of flood-tolerance mechanisms in the Cardamineae tribe.
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van Veen H, Müller JT, Bartylla MM, Akman M, Sasidharan R, and Mustroph A
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- Transcriptome, Rorippa genetics, Rorippa physiology, Gene Expression Profiling, Adaptation, Physiological genetics, Phylogeny, Arabidopsis genetics, Arabidopsis physiology, Cardamine genetics, Cardamine physiology, Floods, Gene Expression Regulation, Plant
- Abstract
Flooding events are highly detrimental to most terrestrial plant species. However, there is an impressive diversity of plant species that thrive in flood-prone regions and represent a treasure trove of unexplored flood-resilience mechanisms. Here we surveyed a panel of four species from the Cardamineae tribe representing a broad tolerance range. This included the flood-tolerant Cardamine pratensis, Rorippa sylvestris and Rorippa palustris and the flood-sensitive species Cardamine hirsuta. All four species displayed a quiescent strategy, evidenced by the repression of shoot growth underwater. Comparative transcriptomics analyses between the four species and the sensitive model species Arabidopsis thaliana were facilitated via de novo transcriptome assembly and identification of 16 902 universal orthogroups at a high resolution. Our results suggest that tolerance likely evolved separately in the Cardamine and Rorippa species. While the Rorippa response was marked by a strong downregulation of cell-cycle genes, Cardamine minimized overall transcriptional regulation. However, a weak starvation response was a universal trait of tolerant species, potentially achieved in multiple ways. It could result from a strong decline in cell-cycle activity, but is also intertwined with autophagy, senescence, day-time photosynthesis and night-time fermentation capacity. Our data set provides a rich source to study adaptational mechanisms of flooding tolerance., (© 2024 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.)
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- 2024
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35. Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum.
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Praat M, Jiang Z, Earle J, Smeekens S, and van Zanten M
- Abstract
Plants must cope with ever-changing temperature conditions in their environment. In many plant species, suboptimal high and low temperatures can induce adaptive mechanisms that allow optimal performance. Thermomorphogenesis is the acclimation to high ambient temperature, whereas cold acclimation refers to the acquisition of cold tolerance following a period of low temperatures. The molecular mechanisms underlying thermomorphogenesis and cold acclimation are increasingly well understood but neither signalling components that have an apparent role in acclimation to both cold and warmth, nor factors determining dose-responsiveness, are currently well defined. This can be explained in part by practical limitations, as applying temperature gradients requires the use of multiple growth conditions simultaneously, usually unavailable in research laboratories. Here we demonstrate that commercially available thermal gradient tables can be used to grow and assess plants over a defined and adjustable steep temperature gradient within one experiment. We describe technical and thermodynamic aspects and provide considerations for plant growth and treatment. We show that plants display the expected morphological, physiological, developmental and molecular responses that are typically associated with high temperature and cold acclimation. This includes temperature dose-response effects on seed germination, hypocotyl elongation, leaf development, hyponasty, rosette growth, temperature marker gene expression, stomatal conductance, chlorophyll content, ion leakage and hydrogen peroxide levels. In conclusion, thermal gradient table systems enable standardized and predictable environments to study plant responses to varying temperature regimes and can be swiftly implemented in research on temperature signalling and response., (© 2024. The Author(s).)
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- 2024
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36. Differential leaf flooding resilience in Arabidopsis thaliana is controlled by ethylene signaling-activated and age-dependent phosphorylation of ORESARA1.
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Rankenberg T, van Veen H, Sedaghatmehr M, Liao CY, Devaiah MB, Stouten EA, Balazadeh S, and Sasidharan R
- Subjects
- Phosphorylation, Plant Senescence genetics, Gene Expression Regulation, Plant, Arabidopsis genetics, Arabidopsis metabolism, Arabidopsis physiology, Ethylenes metabolism, Arabidopsis Proteins metabolism, Arabidopsis Proteins genetics, Plant Leaves metabolism, Plant Leaves genetics, Signal Transduction, Floods, Transcription Factors metabolism, Transcription Factors genetics
- Abstract
The phytohormone ethylene is a major regulator of plant adaptive responses to flooding. In flooded plant tissues, ethylene quickly increases to high concentrations owing to its low solubility and diffusion rates in water. Ethylene accumulation in submerged plant tissues makes it a reliable cue for triggering flood acclimation responses, including metabolic adjustments to cope with flood-induced hypoxia. However, persistent ethylene accumulation also accelerates leaf senescence. Stress-induced senescence hampers photosynthetic capacity and stress recovery. In submerged Arabidopsis, senescence follows a strict age-dependent pattern starting with the older leaves. Although mechanisms underlying ethylene-mediated senescence have been uncovered, it is unclear how submerged plants avoid indiscriminate breakdown of leaves despite high systemic ethylene accumulation. We demonstrate that although submergence triggers leaf-age-independent activation of ethylene signaling via EIN3 in Arabidopsis, senescence is initiated only in old leaves. EIN3 stabilization also leads to overall transcript and protein accumulation of the senescence-promoting transcription factor ORESARA1 (ORE1) in both old and young leaves during submergence. However, leaf-age-dependent senescence can be explained by ORE1 protein activation via phosphorylation specifically in old leaves, independent of the previously identified age-dependent control of ORE1 via miR164. A systematic analysis of the roles of the major flooding stress cues and signaling pathways shows that only the combination of ethylene and darkness is sufficient to mimic submergence-induced senescence involving ORE1 accumulation and phosphorylation. Hypoxia, most often associated with flooding stress in plants, appears to have no role in these processes. Our results reveal a mechanism by which plants regulate the speed and pattern of senescence during environmental stresses such as flooding. Age-dependent ORE1 activity ensures that older, expendable leaves are dismantled first, thus prolonging the life of younger leaves and meristematic tissues that are vital to whole-plant survival., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
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- 2024
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37. High Throughput Image-Based Phenotyping for Determining Morphological and Physiological Responses to Single and Combined Stresses in Potato.
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Abdelhakim LOA, Pleskačová B, Rodriguez-Granados NY, Sasidharan R, Perez-Borroto LS, Sonnewald S, Gruden K, Vothknecht UC, Teige M, and Panzarová K
- Subjects
- Droughts, High-Throughput Screening Assays methods, Solanum tuberosum physiology, Phenotype, Stress, Physiological physiology
- Abstract
High throughput image-based phenotyping is a powerful tool to non-invasively determine the development and performance of plants under specific conditions over time. By using multiple imaging sensors, many traits of interest can be assessed, including plant biomass, photosynthetic efficiency, canopy temperature, and leaf reflectance indices. Plants are frequently exposed to multiple stresses under field conditions where severe heat waves, flooding, and drought events seriously threaten crop productivity. When stresses coincide, resulting effects on plants can be distinct due to synergistic or antagonistic interactions. To elucidate how potato plants respond to single and combined stresses that resemble naturally occurring stress scenarios, five different treatments were imposed on a selected potato cultivar (Solanum tuberosum L., cv. Lady Rosetta) at the onset of tuberization, i.e. control, drought, heat, waterlogging, and combinations of heat, drought, and waterlogging stresses. Our analysis shows that waterlogging stress had the most detrimental effect on plant performance, leading to fast and drastic physiological responses related to stomatal closure, including a reduction in the quantum yield and efficiency of photosystem II and an increase in canopy temperature and water index. Under heat and combined stress treatments, the relative growth rate was reduced in the early phase of stress. Under drought and combined stresses, plant volume and photosynthetic performance dropped with an increased temperature and stomata closure in the late phase of stress. The combination of optimized stress treatment under defined environmental conditions together with selected phenotyping protocols allowed to reveal the dynamics of morphological and physiological responses to single and combined stresses. Here, a useful tool is presented for plant researchers looking to identify plant traits indicative of resilience to several climate change-related stresses.
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- 2024
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38. The soil microbiome modulates the sorghum root metabolome and cellular traits with a concomitant reduction of Striga infection.
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Kawa D, Thiombiano B, Shimels MZ, Taylor T, Walmsley A, Vahldick HE, Rybka D, Leite MFA, Musa Z, Bucksch A, Dini-Andreote F, Schilder M, Chen AJ, Daksa J, Etalo DW, Tessema T, Kuramae EE, Raaijmakers JM, Bouwmeester H, and Brady SM
- Subjects
- Metabolome, Plant Diseases microbiology, Plant Diseases parasitology, Sorghum microbiology, Sorghum metabolism, Striga physiology, Plant Roots microbiology, Plant Roots metabolism, Plant Roots parasitology, Microbiota, Soil Microbiology
- Abstract
Sorghum bicolor is among the most important cereals globally and a staple crop for smallholder farmers in sub-Saharan Africa. Approximately 20% of sorghum yield is lost annually in Africa due to infestation with the root parasitic weed Striga hermonthica. Existing Striga management strategies are not singularly effective and integrated approaches are needed. Here, we demonstrate the functional potential of the soil microbiome to suppress Striga infection in sorghum. We associate this suppression with microbiome-mediated induction of root endodermal suberization and aerenchyma formation and with depletion of haustorium-inducing factors, compounds required for the initial stages of Striga infection. We further identify specific bacterial taxa that trigger the observed Striga-suppressive traits. Collectively, our study describes the importance of the soil microbiome in the early stages of root infection by Striga and pinpoints mechanisms of Striga suppression. These findings open avenues to broaden the effectiveness of integrated Striga management practices., Competing Interests: Declaration of interests A patent application relating to this work has been filed., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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39. A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring.
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Janitza P, Zhu Z, Anwer MU, van Zanten M, and Delker C
- Subjects
- Seedlings metabolism, Vernalization, Hypocotyl, Gene Expression Regulation, Plant, Arabidopsis genetics, Arabidopsis Proteins metabolism
- Abstract
Temperature-induced elongation of hypocotyls, petioles, and roots, together with hyponastic leaf responses, constitute key model phenotypes that can be used to assess a plant's capacity for thermomorphogenesis. Phenotypic responses are often quantified at a single time point during seedling development at different temperatures. However, to capture growth dynamics, several time points need to be assessed, and ideally continuous measurements are taken. Here we describe a general experimental setup and technical solutions for recording and measuring seedling phenotypes at single and multiple time points. Furthermore, we present an R-package called "rootdetectR," which allows easy processing of hypocotyl, root or petiole length, and growth rate data and provides different options of data presentation., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
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- 2024
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40. Impact of individual, combined and sequential stress on photosynthesis machinery in rice (Oryza sativa L).
- Author
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Anwar K, Joshi R, Bahuguna RN, Govindjee G, Sasidharan R, Singla-Pareek SL, and Pareek A
- Subjects
- Humans, Reactive Oxygen Species, Photosynthesis physiology, Chlorophyll, Electron Transport, Photosystem II Protein Complex metabolism, Oryza physiology
- Abstract
Abiotic stresses such as heat, drought and submergence are major threats to global food security. Despite simultaneous or sequential occurrence of these stresses being recurrent under field conditions, crop response to such stress combinations is poorly understood. Rice is a staple food crop for the majority of human beings. Exploitation of existing genetic diversity in rice for combined and/or sequential stress is a useful approach for developing climate-resilient cultivars. We phenotyped ~400 rice accessions under high temperature, drought, or submergence and their combinations. A cumulative performance index revealed Lomello as the best performer across stress and stress combinations at the seedling stage. Lomello showed a remarkable ability to maintain a higher quantum yield of photosystem (PS) II photochemistry. Moreover, the structural integrity of the photosystems, electron flow through both PSI and PSII and the ability to protect photosystems against photoinhibition were identified as the key traits of Lomello across the stress environments. A higher membrane stability and an increased amount of leaf chlorophyll under stress may be due to an efficient management of reactive oxygen species (ROS) at the cellular level. Further, an efficient electron flow through the photosystems and, thus, a higher photosynthetic rate in Lomello is expected to act as a sink for ROS by reducing the rate of electron transport to the high amount of molecular oxygen present in the chloroplast. However, further studies are needed to identify the molecular mechanism(s) involved in the stability of photosynthetic machinery and stress management in Lomello during stress conditions., (© 2024 Scandinavian Plant Physiology Society.)
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- 2024
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41. Subtilase-mediated biogenesis of the expanded family of SERINE RICH ENDOGENOUS PEPTIDES.
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Yang H, Kim X, Skłenar J, Aubourg S, Sancho-Andrés G, Stahl E, Guillou MC, Gigli-Bisceglia N, Tran Van Canh L, Bender KW, Stintzi A, Reymond P, Sánchez-Rodríguez C, Testerink C, Renou JP, Menke FLH, Schaller A, Rhodes J, and Zipfel C
- Subjects
- Serine, Peptides, Protein Kinases genetics, Receptors, Cell Surface genetics, Arabidopsis Proteins genetics, Arabidopsis physiology, Brassicaceae
- Abstract
Plant signalling peptides are typically released from larger precursors by proteolytic cleavage to regulate plant growth, development and stress responses. Recent studies reported the characterization of a divergent family of Brassicaceae-specific peptides, SERINE RICH ENDOGENOUS PEPTIDES (SCOOPs), and their perception by the leucine-rich repeat receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2). Here, we reveal that the SCOOP family is highly expanded, containing at least 50 members in the Columbia-0 reference Arabidopsis thaliana genome. Notably, perception of these peptides is strictly MIK2-dependent. How bioactive SCOOP peptides are produced, and to what extent their perception is responsible for the multiple physiological roles associated with MIK2 are currently unclear. Using N-terminomics, we validate the N-terminal cleavage site of representative PROSCOOPs. The cleavage sites are determined by conserved motifs upstream of the minimal SCOOP bioactive epitope. We identified subtilases necessary and sufficient to process PROSCOOP peptides at conserved cleavage motifs. Mutation of these subtilases, or their recognition motifs, suppressed PROSCOOP cleavage and associated overexpression phenotypes. Furthermore, we show that higher-order mutants of these subtilases show phenotypes reminiscent of mik2 null mutant plants, consistent with impaired PROSCOOP biogenesis, and demonstrating biological relevance of SCOOP perception by MIK2. Together, this work provides insights into the molecular mechanisms underlying the functions of the recently identified SCOOP peptides and their receptor MIK2., (© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
- Published
- 2023
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42. 25 Years of thermomorphogenesis research: milestones and perspectives.
- Author
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Quint M, Delker C, Balasubramanian S, Balcerowicz M, Casal JJ, Castroverde CDM, Chen M, Chen X, De Smet I, Fankhauser C, Franklin KA, Halliday KJ, Hayes S, Jiang D, Jung JH, Kaiserli E, Kumar SV, Maag D, Oh E, Park CM, Penfield S, Perrella G, Prat S, Reis RS, Wigge PA, Willige BC, and van Zanten M
- Subjects
- Gene Expression Regulation, Plant, Temperature, Hypocotyl metabolism, Indoleacetic Acids, Arabidopsis Proteins metabolism, Arabidopsis genetics, Arabidopsis metabolism
- Abstract
In 1998, Bill Gray and colleagues showed that warm temperatures trigger arabidopsis hypocotyl elongation in an auxin-dependent manner. This laid the foundation for a vibrant research discipline. With several active members of the 'thermomorphogenesis' community, we here reflect on 25 years of elevated ambient temperature research and look to the future., Competing Interests: Declaration of interests No interests are declared., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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43. The genetic basis of plants' battle against witchweeds: linking immune responses to distinct resistance mechanisms.
- Author
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Jhu MY, Kawa D, and Brady SM
- Subjects
- Immunity, Striga
- Published
- 2023
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44. Parallels between drought and flooding: An integrated framework for plant eco-physiological responses to water stress.
- Author
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Chen S, Ten Tusscher KHWJ, Sasidharan R, Dekker SC, and de Boer HJ
- Abstract
Drought and flooding occur at opposite ends of the soil moisture spectrum yet their resulting stress responses in plants share many similarities. Drought limits root water uptake to which plants respond with stomatal closure and reduced leaf gas exchange. Flooding limits root metabolism due to soil oxygen deficiency, which also limits root water uptake and leaf gas exchange. As drought and flooding can occur consecutively in the same system and resulting plant stress responses share similar mechanisms, a single theoretical framework that integrates plant responses over a continuum of soil water conditions from drought to flooding is attractive. Based on a review of recent literature, we integrated the main plant eco-physiological mechanisms in a single theoretical framework with a focus on plant water transport, plant oxygen dynamics, and leaf gas exchange. We used theory from the soil-plant-atmosphere continuum modeling as "backbone" for our framework, and subsequently incorporated interactions between processes that regulate plant water and oxygen status, abscisic acid and ethylene levels, and the resulting acclimation strategies in response to drought, waterlogging, and complete submergence. Our theoretical framework provides a basis for the development of mathematical models to describe plant responses to the soil moisture continuum from drought to flooding., Competing Interests: All co‐authors have no conflict of interest to declare., (© 2023 The Authors. Plant‐Environment Interactions published by New Phytologist Foundation and John Wiley & Sons Ltd.)
- Published
- 2023
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45. Is hypoxia good or bad? Role of oxygen levels in maize kernel development.
- Author
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Sanclemente MA
- Subjects
- Seeds genetics, Genes, Plant, Hypoxia, Zea mays genetics, Oxygen
- Abstract
Competing Interests: Conflict of interest statement. None declared.
- Published
- 2023
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46. The Mediator complex subunit MED25 interacts with HDA9 and PIF4 to regulate thermomorphogenesis.
- Author
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Shapulatov U, van Zanten M, van Hoogdalem M, Meisenburg M, van Hall A, Kappers I, Fasano C, Facella P, Loh CC, Perrella G, and van der Krol A
- Subjects
- Mediator Complex genetics, Mediator Complex metabolism, Transcription Factors genetics, Transcription Factors metabolism, Histone Deacetylases genetics, Histone Deacetylases metabolism, Gene Expression Regulation, Plant, Basic Helix-Loop-Helix Transcription Factors genetics, Basic Helix-Loop-Helix Transcription Factors metabolism, Arabidopsis Proteins genetics, Arabidopsis Proteins metabolism, Phytochrome metabolism, Arabidopsis metabolism
- Abstract
Thermomorphogenesis is, among other traits, characterized by enhanced hypocotyl elongation due to the induction of auxin biosynthesis genes like YUCCA8 by transcription factors, most notably PHYTOCHROME INTERACTING FACTOR 4 (PIF4). Efficient binding of PIF4 to the YUCCA8 locus under warmth depends on HISTONE DEACETYLASE 9 (HDA9) activity, which mediates histone H2A.Z depletion at the YUCCA8 locus. However, HDA9 lacks intrinsic DNA-binding capacity, and how HDA9 is recruited to YUCCA8, and possibly other PIF4-target sites, is currently not well understood. The Mediator complex functions as a bridge between transcription factors bound to specific promoter sequences and the basal transcription machinery containing RNA polymerase II. Mutants of Mediator component Mediator25 (MED25) exhibit reduced hypocotyl elongation and reduced expression of YUCCA8 at 27°C. In line with a proposed role for MED25 in thermomorphogenesis in Arabidopsis (Arabidopsis thaliana), we demonstrated an enhanced association of MED25 to the YUCCA8 locus under warmth and interaction of MED25 with both PIF4 and HDA9. Genetic analysis confirmed that MED25 and HDA9 operate in the same pathway. Intriguingly, we also showed that MED25 destabilizes HDA9 protein. Based on our findings, we propose that MED25 recruits HDA9 to the YUCCA8 locus by binding to both PIF4 and HDA9., Competing Interests: Conflict of interest statement. None declared., (© The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
- Published
- 2023
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47. Plant responses to limited aeration: Advances and future challenges.
- Author
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Dalle Carbonare L, Jiménez JC, Lichtenauer S, and van Veen H
- Abstract
Limited aeration that is caused by tissue geometry, diffusion barriers, high elevation, or a flooding event poses major challenges to plants and is often, but not exclusively, associated with low oxygen. These processes span a broad interest in the research community ranging from whole plant and crop responses, post-harvest physiology, plant morphology and anatomy, fermentative metabolism, plant developmental processes, oxygen sensing by ERF-VIIs, gene expression profiles, the gaseous hormone ethylene, and O
2 dynamics at cellular resolution. The International Society for Plant Anaerobiosis (ISPA) gathers researchers from all over the world contributing to understand the causes, responses, and consequences of limited aeration in plants. During the 14th ISPA meeting, major research progress was related to the evolution of O2 sensing mechanisms and the intricate network that balances low O2 signaling. Here, the work moved beyond flooding stress and emphasized novel underexplored roles of low O2 and limited aeration in altitude adaptation, fruit development and storage, and the vegetative development of growth apices. Regarding tolerance towards flooding, the meeting stressed the relevance and regulation of developmental plasticity, aerenchyma, and barrier formation to improve internal aeration. Additional newly explored flood tolerance traits concerned resource balance, senescence, and the exploration of natural genetic variation for novel tolerance loci. In this report, we summarize and synthesize the major progress and future challenges for low O2 and aeration research presented at the conference., Competing Interests: The Authors did not report any conflict of interest., (© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)- Published
- 2023
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48. Multi-stress resilience in plants recovering from submergence.
- Author
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Yuan LB, Chen MX, Wang LN, Sasidharan R, Voesenek LACJ, and Xiao S
- Subjects
- Floods, Adaptation, Physiological, Plants, Oryza metabolism, Arabidopsis physiology
- Abstract
Submergence limits plants' access to oxygen and light, causing massive changes in metabolism; after submergence, plants experience additional stresses, including reoxygenation, dehydration, photoinhibition and accelerated senescence. Plant responses to waterlogging and partial or complete submergence have been well studied, but our understanding of plant responses during post-submergence recovery remains limited. During post-submergence recovery, whether a plant can repair the damage caused by submergence and reoxygenation and re-activate key processes to continue to grow, determines whether the plant survives. Here, we summarize the challenges plants face when recovering from submergence, primarily focusing on studies of Arabidopsis thaliana and rice (Oryza sativa). We also highlight recent progress in elucidating the interplay among various regulatory pathways, compare post-hypoxia reoxygenation between plants and animals and provide new perspectives for future studies., (© 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
- Published
- 2023
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49. The root-knot nematode effector MiMSP32 targets host 12-oxophytodienoate reductase 2 to regulate plant susceptibility.
- Author
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Verhoeven A, Finkers-Tomczak A, Prins P, Valkenburg-van Raaij DR, van Schaik CC, Overmars H, van Steenbrugge JJM, Tacken W, Varossieau K, Slootweg EJ, Kappers IF, Quentin M, Goverse A, Sterken MG, and Smant G
- Subjects
- Animals, Oxidoreductases metabolism, Biological Transport, Plant Diseases, Oxidoreductases Acting on CH-CH Group Donors metabolism, Tylenchoidea physiology
- Abstract
To establish persistent infections in host plants, herbivorous invaders, such as root-knot nematodes, must rely on effectors for suppressing damage-induced jasmonate-dependent host defenses. However, at present, the effector mechanisms targeting the biosynthesis of biologically active jasmonates to avoid adverse host responses are unknown. Using yeast two-hybrid, in planta co-immunoprecipitation, and mutant analyses, we identified 12-oxophytodienoate reductase 2 (OPR2) as an important host target of the stylet-secreted effector MiMSP32 of the root-knot nematode Meloidogyne incognita. MiMSP32 has no informative sequence similarities with other functionally annotated genes but was selected for the discovery of novel effector mechanisms based on evidence of positive, diversifying selection. OPR2 catalyzes the conversion of a derivative of 12-oxophytodienoate to jasmonic acid (JA) and operates parallel to 12-oxophytodienoate reductase 3 (OPR3), which controls the main pathway in the biosynthesis of jasmonates. We show that MiMSP32 targets OPR2 to promote parasitism of M. incognita in host plants independent of OPR3-mediated JA biosynthesis. Artificially manipulating the conversion of the 12-oxophytodienoate by OPRs increases susceptibility to multiple unrelated plant invaders. Our study is the first to shed light on a novel effector mechanism targeting this process to regulate the susceptibility of host plants., (© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.)
- Published
- 2023
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50. Plasticity of maternal environment-dependent expression-QTLs of tomato seeds.
- Author
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Sterken MG, Nijveen H, van Zanten M, Jiménez-Gómez JM, Geshnizjani N, Willems LAJ, Rienstra J, Hilhorst HWM, Ligterink W, and Snoek BL
- Subjects
- Plant Breeding, Quantitative Trait Loci, Chromosome Mapping, Seeds genetics, Seedlings genetics, Solanum lycopersicum
- Abstract
Seeds are essential for plant reproduction, survival, and dispersal. Germination ability and successful establishment of young seedlings strongly depend on seed quality and on environmental factors such as nutrient availability. In tomato (Solanum lycopersicum) and many other species, seed quality and seedling establishment characteristics are determined by genetic variation, as well as the maternal environment in which the seeds develop and mature. The genetic contribution to variation in seed and seedling quality traits and environmental responsiveness can be estimated at transcriptome level in the dry seed by mapping genomic loci that affect gene expression (expression QTLs) in contrasting maternal environments. In this study, we applied RNA-sequencing to construct a linkage map and measure gene expression of seeds of a tomato recombinant inbred line (RIL) population derived from a cross between S. lycopersicum (cv. Moneymaker) and S. pimpinellifolium (G1.1554). The seeds matured on plants cultivated under different nutritional environments, i.e., on high phosphorus or low nitrogen. The obtained single-nucleotide polymorphisms (SNPs) were subsequently used to construct a genetic map. We show how the genetic landscape of plasticity in gene regulation in dry seeds is affected by the maternal nutrient environment. The combined information on natural genetic variation mediating (variation in) responsiveness to the environment may contribute to knowledge-based breeding programs aiming to develop crop cultivars that are resilient to stressful environments., (© 2023. The Author(s).)
- Published
- 2023
- Full Text
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