Search

Your search keyword '"Pla Palacin, Iris"' showing total 14 results
Start Over Author "Pla Palacin, Iris"
14 results on '"Pla Palacin, Iris"'

1. Polymorphisms Associated With Metabolic Dysfunction-Associated Steatotic Liver Disease Influence the Progression of End-Stage Liver Disease

Author

Kocas-Kilicarslan, Zehra N., Cetin, Zeliha, Faccioli, Lanuza A.P., Motomura, Takashi, Amirneni, Sriram, Diaz-Aragon, Ricardo, Florentino, Rodrigo M., Sun, Yiyue, Pla-Palacin, Iris, Xia, Mengying, Miedel, Mark T., Kurihara, Takeshi, Hu, Zhiping, Ostrowska, Alina, Wang, Zi, Constantine, Robert, Li, Albert, Taylor, D. Lansing, Behari, Jaideep, Soto-Gutierrez, Alejandro, and Tafaleng, Edgar N.

Published
2024
Full Text
View/download PDF

2. Liver Tissue Engineering

Author

Pla-Palacín, Iris, Sánchez-Romero, Natalia, Morini, Sara, Rubio-Soto, Daniela, Baptista, Pedro M., Eberli, Daniel, editor, Lee, Sang Jin, editor, and Traweger, Andreas, editor

Published
2021
Full Text
View/download PDF

3. Tissue Organoids: Liver

Author

Solanas, Estela, Pla-Palacín, Iris, Sainz-Arnal, Pilar, Almeida, Manuel, Lue, Alberto, Serrano, Trinidad, Baptista, Pedro M., Teicher, Beverly A., Series editor, Soker, Shay, editor, and Skardal, Aleksander, editor

Published
2018
Full Text
View/download PDF

4. Naturally-Derived Biomaterials for Tissue Engineering Applications

Author

Brovold, Matthew, Almeida, Joana I., Pla-Palacín, Iris, Sainz-Arnal, Pilar, Sánchez-Romero, Natalia, Rivas, Jesus J., Almeida, Helen, Dachary, Pablo Royo, Serrano-Aulló, Trinidad, Soker, Shay, Baptista, Pedro M., COHEN, IRUN R., Series Editor, LAJTHA, ABEL, Series Editor, LAMBRIS, JOHN D., Series Editor, PAOLETTI, RODOLFO, Series Editor, Rezaei, Nima, Series Editor, Chun, Heung Jae, editor, Park, Kwideok, editor, Kim, Chun-Ho, editor, and Khang, Gilson, editor

Published
2018
Full Text
View/download PDF

7. Large-scale Production of LGR5-positive Bipotential Human Liver Stem Cells.

Author

Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, Spee, Bart, Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, and Spee, Bart

Abstract

The gap between patients on transplant waiting lists and available donor organs is steadily increasing. Human organoids derived from Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-positive adult stem cells represent an exciting new cell source for liver regeneration; however, culturing large numbers of organoids with current protocols is tedious and the level of hepatic differentiation is limited. Here, we established a new method for the expansion of large quantities of human liver organoids in spinner flasks. Due to improved oxygenation in the spinner flasks, organoids rapidly proliferated and reached an average 40-fold cell expansion after two weeks, compared to 6-fold expansion in static cultures. The organoids repopulated decellularized liver discs and formed liver-like tissue. After differentiation in spinner flasks, mature hepatocyte markers were highly upregulated compared to static organoid cultures, and cytochrome p450 activity reached levels equivalent to hepatocytes. CONCLUSION: We established a highly efficient method for culturing large numbers of LGR5-positive stem cells in the form of organoids, which paves the way for the application of organoids for tissue engineering and liver transplantation.

Published
2020

8. Large-scale Production of LGR5-positive Bipotential Human Liver Stem Cells.

Author

Onderzoek, dCSCA RMSC-1, Sub General Pharmacology, dCSCA AVR, Biochemisch laboratorium, Faculteit Diergeneeskunde, Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, Spee, Bart, Onderzoek, dCSCA RMSC-1, Sub General Pharmacology, dCSCA AVR, Biochemisch laboratorium, Faculteit Diergeneeskunde, Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, and Spee, Bart

Published
2020

9. Large-scale Production of LGR5-positive Bipotential Human Liver Stem Cells

Author

MDL onderzoek 2, Onderzoek Precision medicine, Metabole ziekten onderzoek 1, Metabole ziekten patientenzorg, Regenerative Medicine and Stem Cells, Child Health, CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, Spee, Bart, MDL onderzoek 2, Onderzoek Precision medicine, Metabole ziekten onderzoek 1, Metabole ziekten patientenzorg, Regenerative Medicine and Stem Cells, Child Health, CMM Sectie Molecular Cancer Research, Cancer, Hubrecht Institute with UMC, Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, and Spee, Bart

Published
2020

11. Large‐Scale Production of LGR5‐Positive Bipotential Human Liver Stem Cells

Author

Schneeberger, Kerstin, primary, Sánchez‐Romero, Natalia, additional, Ye, Shicheng, additional, van Steenbeek, Frank G., additional, Oosterhoff, Loes A., additional, Pla Palacin, Iris, additional, Chen, Chen, additional, van Wolferen, Monique E., additional, van Tienderen, Gilles, additional, Lieshout, Ruby, additional, Colemonts‐Vroninks, Haaike, additional, Schene, Imre, additional, Hoekstra, Ruurdtje, additional, Verstegen, Monique M.A., additional, van der Laan, Luc J.W., additional, Penning, Louis C., additional, Fuchs, Sabine A., additional, Clevers, Hans, additional, De Kock, Joery, additional, Baptista, Pedro M., additional, and Spee, Bart, additional

Published
2020
Full Text
View/download PDF

12. Large-scale Production of LGR5-positive Bipotential Human Liver Stem Cells

Author

Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, Spee, Bart, Schneeberger, Kerstin, Sánchez-Romero, Natalia, Ye, Shicheng, van Steenbeek, Frank G, Oosterhoff, Loes A, Pla Palacin, Iris, Chen, Chen, van Wolferen, Monique E, van Tienderen, Gilles, Lieshout, Ruby, Colemonts-Vroninks, Haaike, Schene, Imre, Hoekstra, Ruurdtje, Verstegen, Monique M A, van der Laan, Luc J W, Penning, Louis C, Fuchs, Sabine A, Clevers, Hans, De Kock, Joery, Baptista, Pedro M, and Spee, Bart

Abstract

The gap between patients on transplant waiting lists and available donor organs is steadily increasing. Human organoids derived from Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5)-positive adult stem cells represent an exciting new cell source for liver regeneration; however, culturing large numbers of organoids with current protocols is tedious and the level of hepatic differentiation is limited. Here, we established a new method for the expansion of large quantities of human liver organoids in spinner flasks. Due to improved oxygenation in the spinner flasks, organoids rapidly proliferated and reached an average 40-fold cell expansion after two weeks, compared to 6-fold expansion in static cultures. The organoids repopulated decellularized liver discs and formed liver-like tissue. After differentiation in spinner flasks, mature hepatocyte markers were highly upregulated compared to static organoid cultures, and cytochrome p450 activity reached levels equivalent to hepatocytes. CONCLUSION: We established a highly efficient method for culturing large numbers of LGR5-positive stem cells in the form of organoids, which paves the way for the application of organoids for tissue engineering and liver transplantation.

Published
2019

13. Contributors

Author

Agarwal, Rachit, Ahlstrom, Jon D., Ahmad, Rafiq, Alarcon, Emilio I., Almarza, Alejandro J., Almeida-Porada, Graça, Almeida, Manuel, Alvarado-Velez, Melissa, Anderson, James M., Arcidiacono, Judith, Atala, Anthony, Badylak, Stephen F., Balkan, Wayne, Ballios, Brian G., Baptista, Pedro M., Baumann, M. Douglas, Bedi, Supinder S., Bellamkonda, Ravi V., Bergmann, Nicole M., Blau, Helen M., Boerckel, Joel D., Bratt-Leal, Andres M., Brown, James C., Brubaker, Scott, Brunette, Isabelle, Calderon, Gisele A., Caplan, Arnold I., Castner, David G., Chang, Cynthia, Chawla, Aditya, Chen, Xuguang, Cohen, Paul, Cooke, Michael J., Copus, Joshua S., Correlo, Vitor M., Cox, Charles S., Jr., Dahl, Abritee, Day, Richard M., De Coppi, Paolo, Dodla, Mahesh C., Elisseeff, Jennifer H., Emamaullee, Juliet A., Esa, Adam, Fang, Yunlan, Faust, Heather J., Fisher, John P., Fisher, Matthew B., Francois, Elvis L., García, Andrés J., Gavrilov, Svetlana, Gazit, Dan, Gazit, Zulma, Gemmiti, Christopher V., Gillispie, Gregory J., Gilpin, Sarah E., Godbey, W.T., Gray, Andrea, Green, Ronald M., Griffith, May, Guldberg, Robert E., Guo, Qiongyu, Gurtner, Geoffrey C., Hacker, Michael C., Hanna, Issa A., Hare, Joshua M., Hatzistergos, Konstantinos E., Herber, Ralf-Peter, Hilborn, Jöns, Humes, H. David, Hunsberger, Joshua G., Iwasa, Kenjiro, Jackson, John D., Jackson, Margaret L., Jang, Hae Lin, Jansen, John A., Johnston, Josephine, Jorns, Carl, Kang, Huijun, Kaplan, David L., Kaplan, David S., Katz, Adam J., Kelley, Matthew W., Kennedy, Kelsey, Khademhosseini, Ali, Khang, Gilson, Kim, Jinho, Klein, Rachel H., Klimanskaya, Irina, Knoepfler, Paul S., Ko, In Kap, Kolambkar, Yash M., Krieghoff, Jan, Kucko, Nathan W., Kumar, Manoj, Kurtzberg, Joanne, Kwilas, Anna, Landry, Donald W., Langhans, Mark T., Lanza, Robert, Lanzoni, Giacomo, Lee, Sang Jin, Leeuwenburgh, Sander C.G., Leong, Kam W., Liang, Rui, Liaudanskaya, Volha, Lin, Hang, Longaker, Michael T., Lorenz, Hermann P., Loring, Jeanne F., Lu, Shi-Jiang, Lue, Alberto, Ma, Peter X., Magalhaes, Renata S., Mandla, Serena, Marshall, Clement D., Martins-Green, Manuela, Mason, Devon E., Mau, Jonquil R., McFarland, Richard, McHale, Melissa K., Melville, James C., Meyers, Jason R., Mikos, Antonios G., Miller, Jordan S., Mittermiller, Paul A., Miyachi, Hideki, Miyamoto, Shinka, Monteiro, Nelson, Moore, Alessandra L., Morini, Sara, Moser, Philipp T., Mukhatyar, Vivek J., Murdock, Mark, Nagiel, Aaron, Naughton, Gail K., Nauta, Allison, Navarro, Javier, Newton, Jared M., Nori, Aparna, Okano, Teruo, Oliveira, Joaquim M., Ott, Harald C., Padmanabhan, Jagannath, Page, Kristin M., Pallickaveedu Rajan Asari, Anil Kumar, Papaioannou, Virginia E., Park, Jihoon, Payne, Samantha L., Pelled, Gadi, Pepper, Andrew, Perumal, Elumalai, Petreaca, Melissa, Pino, Christopher J., Pirosa, Alessandro, Pla-Palacín, Iris, Pokrywczynska, Marta, Porada, Christopher D., Porter, Blaise D., Radisic, Milica, Rambhia, Kunal J., Raquel Maia, F., Ratner, Buddy D., Reddi, A.H., Reis, Rui L., Ricles, Laura, Ricordi, Camillo, Rizwan, Muhammad, Robinson, Rebecca, Rodrigues, Melanie, Rothrauff, Benjamin B., Sadri-Ardekani, Hooman, Sainz-Arnal, Pilar, Sambathkumar, Rangarajan, Sánchez-Romero, Natalia, Scarritt, Michelle, Schneider, Christopher M., Schwartz, Steven D., Selem, Sarah, Serrano-Aulló, Trinidad, Shapiro, A.M. James, Sheyn, Dmitriy, Shimizu, Tatsuya, Shinoka, Toshiharu, Shoichet, Molly S., Shoji, Toshihiro, Shupe, Thomas, Sikora, Andrew G., Simpson, Fiona, Skardal, Aleksander, Skuk, Daniel, Smith, Brandon T., Sohn, Jihee, Soker, Shay, Solanas, Estela, Song, Jeong Eun, Sood, Disha, Stocum, David L., Strom, Stephen C., Sun, Jessica M., Takahashi, Hironobu, Tremblay, Jacques P., Tripathy, Nirmalya, Tse, John W., Tuan, Rocky S., Verfaillie, Catherine M., Vunjak-Novakovic, Gordana, Wagner, William R., Wang, Yanling, Watson, Emma, West, Jennifer L., Williams, David F., Williams, James K., Wong, Mark E., Woo, Savio L-Y., Wood, Fiona M., Xu, Lei, Yakubovich, Doron C., Yang, Yafeng, Yaszemski, Michael J., Yelick, Pamela C., Yim, Evelyn K.F., Yong, Carolyn, Yoo, James J., Young, Simon, Yucel, Nora, Zacharias, Rachel L., Zhang, Yuanyuan, Zhang, Ai, Zhang, Jin, and Zhu, Yang

Published
2019
Full Text
View/download PDF

14. In vivo transplantation of intrahepatic cholangiocyte organoids with decellularized liver-derived hydrogels supports hepatic cellular proliferation and differentiation in chronic liver injury.

Author

Kaur I, Vasudevan A, Sanchez-Romero N, Sanyal A, Sharma A, Hemati H, Juneja P, Sharma A, Pla Palacin I, Rastogi A, Vijayaragavan P, Ghosh S, Ramakrishna S, Sarin SK, Baptista PM, Tripathi DM, and Kaur S

Abstract

The limited replicative potential of primary hepatocytes (Hep) is a major hurdle for obtaining sufficient quantity and quality hepatocytes during cell therapy in patients with liver failure. Intrahepatic cholangiocyte organoids (ICOs) derived from intrahepatic bile ducts differentiate into both hepatocytes and cholangiocytes in vitro . Here, we studied in vivo effects of transplanting ICOs and Hep in chronic liver injury mice models. Well characterized primary mouse ICOs and Hep were mixed in decellularized liver (DCL) matrix hydrogels and injected into the subcapsular left lateral liver lobe of CCl 4 -induced liver injury models whereas mice given DCL alone were in the sham group. Two weeks post-transplantation, transplanted liver lobes were collected and studied by histology and RNA sequencing. Transplanted animals did not exhibit any tumors, mortality or morbidity. Mice livers transplanted with ICOs had increased cellular proliferation and vascularization as compared to Hep transplanted mice or sham. Collagen deposition in the liver was significantly reduced and serum albumin levels were significantly increased in transplanted groups compared to the sham group. Expression of genes associated with hepatocyte differentiation was highest in Hep transplanted livers among the three groups, but they were also upregulated in ICO transplanted livers compared to sham. Our study demonstrates that ICOs encapsulated in DCL hydrogels when transplanted in chronically injured mice livers engraft well and show hepatocyte differentiation and reduction of fibrosis, indicating that hydrogel transplanted cholangiocyte organoids may serve as an efficient cell source and therapy for renewal of hepatocytes, restoration of hepatocyte functions and resolution of liver injury.

Published
2024
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results