13 results on '"Pittino, M"'
Search Results
2. Increased content of CD34+CD38− hematopoietic stem cells in the last collected umbilical cord blood
- Author
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Malangone, W, Belvedere, O, Astori, G, Adami, V, Donini, A, Falasca, E, Sala, P.G, Del Frate, G, Pittino, M, De Anna, D, and Degrassi, A
- Published
- 2001
- Full Text
- View/download PDF
3. Adhesions and pain in women with first diagnosis of endometriosis: Results from a cross-sectional study
- Author
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Busacca, M., Mais, V. R., Parazzini, F., Venturini, P. L., Cipriani, S., Ciavattini, A., Baiocchi, R., Scollo, P., Muriana, A., Campione, S., Guazzelli, G., Spolaor, L., Bianchi, A., Martinelli, R., Bracco, G. L., Coccia, M. E., Aretini, M., Franchini, M., Allegra, S. P., Meroni, N., Radaelli, U., Bertulessi, C., Vignali, M., Campagna, A., Pieroni, A., Dal Pozzo, G., Cobellis, I., Trossarelli, G. F., Zingaro, L., Gerli, S., Baiocchi, G., Ceccarelli, T., Di Nisio, Q., De Simone, P., Francesc A., Di, Cela, V., Genazzani, A. R., Liguori, S., Sesti, F., Piccione, E., Bonifacio, S., Virra, E., Corrado, G., Porpora, Maria Grazia, Pittino, M., Del Frate, G., Dessole, S., Capobianco, G., Multinu, A., Micalef, S., Seiner, P., Marchino, G. L., Gennarelli, G., De Seta, F., Guaschino, S., Troiano, L., Beretta, P., and Gruppo Italiano Di Studio Dell'Endometriosi, Bernasconi L.
- Subjects
Adult ,endometriosis ,medicine.medical_specialty ,Adolescent ,Cross-sectional study ,Endometriosis ,Tissue Adhesions ,chronic pelvic pain ,Pelvic Pain ,Peritoneal Diseases ,adhesions ,localization ,pain ,stage ,Statistics, Nonparametric ,Surveys and Questionnaires ,medicine ,Humans ,Ovarian Diseases ,Stage (cooking) ,Pain Measurement ,Chi-Square Distribution ,Obstetrics ,business.industry ,Pelvic pain ,Obstetrics and Gynecology ,Middle Aged ,medicine.disease ,Cross-Sectional Studies ,Female ,Observational study ,medicine.symptom ,business - Abstract
To analyze the relationship between pain and presence, site, and type of adhesions in women with endometriosis.This was a multicenter, observational cross-sectional study. Eligible for the study were women with endometriosis and pelvic pain, consecutively observed during the study period at the collaborating centers. A total of 574 women entered the study.Adhesions were observed in 81.9% of cases (470 women). The frequency was lower in women with endometriosis at stage I-II (65%) and higher in women with stage III-IV (88%); this difference was statistically significant (p.01). The frequency of adhesions was lower in women with ovarian endometriosis (74%) and higher in those with ovarian and peritoneal endometriosis (87%) or other sites (96%) (p.01). The presence of adhesions was associated with higher mean visual analog scale (VAS) scores and median multidimensional scale ratings in women with ovarian disease and with stage I-II disease. Women with ovarian adhesions reported higher VAS and multidimensional scale scores (p.05) than women with peritoneal adhesions or adhesions in other sites.The results of this study suggest that there is no overall association between the presence of adhesions and the degree of pain in women with endometriosis. The data suggests that there may be an association between adhesions and pain in women with ovarian and Type I-II endometriosis. However, these associations were no longer significant after correction for multiple comparisons. Further research is indicated to test these associations.
- Published
- 2006
4. Attitudes Toward Randomized, Controlled Trials in Surgery: Opinions of Gynecologists of the Gruppo Italiano di Studio sull'Endometriosi
- Author
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Aguzzoli, L., Alio, L., Aretini, M., Barletta, F., Beretta, P., Bertulessi, C., Bianchi, A., Bracco, G. L., Busacca, M., Cappiello, F., Casa, ., Cela, V., Ciavattini, A., Congiu, M. A., Consonni, R., Dal, Pozzo, Del Frate, G., Cristiano, A. D., Di Dionisio, A., Dolcetta, G., Filliolini, C., Genazzani, A. R., Gerli, S., Giambanco, V., La Greca, M., La Grotta, F., Litta, PIETRO SALVATORE, Mais, V., Mariani, L., Martinello, R., Martulli, B., Mazzini, M., Meroni, N., Micalef, S., Montanino, O. M., Muriana, A., Pasini, A., Pieroni, A., Pittino, M., Porpora, M. G., Puglisi, A. S., Saccucci, P., Santuz, M., Scollo, P., Sesti, F., Stola, E., Tinelli, F., Vicino, M., Volpi, E., Zupi, E., and Parazinni, F.
- Subjects
endometriosis ,treatment planning ,medicine.medical_specialty ,Alternative medicine ,randomization ,surgical technique ,medical research ,law.invention ,gynecologic surgery ,Randomized controlled trial ,law ,medicine ,controlled study ,human ,normal human ,skill ,Random allocation ,business.industry ,article ,Obstetrics and Gynecology ,financial management ,Surgical procedures ,Surgery ,priority journal ,attitude ,business - Abstract
In order to assess barriers and the attitudes of gynecologists toward randomized controlled trials (RCT) in surgery for endometriosis, we identified 62 gynecologists affiliated with the Gruppo Italiano di Studio sull'Endometriosi and sent them a questionnaire. A total of 52 (83.9%) gynecologists completed and sent it back to the coordinating center. Most gynecologists strongly agreed or agreed that RCTs should be the study design of choice for evaluating surgical procedures, but 66% also strongly agree/agree that RCTs are best suited to nonsurgical setting. With regard to the feasibility of RCT in surgery, there was a high level (>50%) of agreement with the statement: RCTs are insufficiently funded; the surgical community gives to RCTs too low priority; patients are unlikely to accept random allocation. A total of 43% agree or strongly agree that there is often consensus on new procedures without the need for RCTs, but 49% disagree or strongly disagree. Considering the statements on methodological problem...
- Published
- 2004
5. Determinants of adenomyosis in women who underwent hysterectomy for benign gynecological conditions: results from a prospective multicentric study in Italy
- Author
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Parazzini, F, Mais, V, Cipriani, S, Busacca, M, Venturini, P, Ciavattini, A, Baiocchi, R, Goteri, G, Bertivagni, E, Biggio, A, Muriana, Palomba, S, Zullo, F, Bianchi, A, Martinello, R, Bracco, Gl, Rizzitello, F, Coccia, E, Meroni, N, Radaelli, U, Spreafico, C, Odorizzi, Mp, Bertazzoli, E, Cannata, L, Pieroni, G, Vizza, E, Cosmaggioni, I, Pittino, M, DEL FRATE, G, Trossarelli, Gf, Chiapparo, F, Beretta, P, Ghezzi, Fabio, Cromi, Antonella, Uccella, Stefano, Piccione, E, DI CIACCI, S, Petropolli, A, Sesti, F, Micalef, S, Pecori, E, Cobellis, L, Palermo, Mr, Surico, N, Leo, L, Surico, D, Boninfante, G, Negri, Noci, Rosales, Noci, Parazzini, F, Mais, V, Cipriani, S, Busacca, M, Venturini, P, Ciavattini, A, Baiocchi, R, Goteri, G, Bertivagni, E, Biggio, A, Muriana, Palomba, S, Zullo, F, Bianchi, A, Martinello, R, Bracco, Gl, Rizzitello, F, Coccia, E, Meroni, N, Radaelli, U, Spreafico, C, Odorizzi, Mp, Bertazzoli, E, Cannata, L, Pieroni, G, Vizza, E, Cosmaggioni, I, Pittino, M, DEL FRATE, G, Trossarelli, Gf, Chiapparo, F, Beretta, P, Ghezzi, F, Cromi, A, Uccella, S, Piccione, E, DI CIACCI, S, Petropolli, A, Sesti, F, Micalef, S, Pecori, E, Cobellis, Luigi, Palermo, Mr, Surico, N, Leo, L, Surico, D, Boninfante, G, Negri, Noci, and Rosales, Noci
- Subjects
Epidemiology ,Risk factor ,Adenomyosi - Published
- 2009
6. Relationship between stage, site and morphological characteristics of pelvic endometriosis and pain
- Author
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Parazzini, F., Cipriani, S., Moroni, S., Crosignani, P. G., Ciavattini, A., Garzetti, G., Dolcetta, G., Scollo, M., Vicino, M., Loverro, G., Sabatelli, S., Decca, L., Falsetti, L., Giacomucci, E., Flamigni, C., Mais, V., Guerriero, S., Boscia, F., Sangiorgio, G., Scollo, P., Muriana, A., La Greca, M., Distefano, C., Belloni, C., Spolaor, L., Bianchi, A., Aretini, M., Franchini, M., Bracco, G. L., Coccia, M. E., Scarselli, G. F., Ciuffreda, F., Fiscella, C., Tinelli, F., Demarzi, C. A., Bianco, B., Iannelli, A., Radaelli, U., Meroni, N., Federici, D., Calia, C., Vercellini, P., Bertulessi, C., Hanozet, F., Busacca, M., Dal Pozzo, G., Pieroni, A., Lita, P., Bracciante, R., Baiocchi, G., Congiu, M. A., Fanfani, R., Sesti, F., Bonifacio, S., Porpora, M. G., Pittino, M., Del Frate, G., Dessole, S., Capobianco, G., Montanino Oliva, M., Primilerio, M., Micalef, S., Ansaldi, E., Massobrio, M., Guidetti, D., Rosati, M., Di Dionisio, A., Bracalente, G., Guaschino, S., Troiano, L., Francesco De Seta, Santuz, M., Petraglia, F., Canducci, E., Beretta, P., and Santo, D.
- Subjects
Stage ,Endometriosis ,Pain ,Site ,Settore MED/40 - Ginecologia e Ostetricia - Published
- 2001
7. Increased content of CD34+CD38−hematopoietic stem cells in the last collected umbilical cord blood
- Author
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Malangone, W, Belvedere, O, Astori, G, Adami, V, Donini, A, Falasca, E, Sala, P.G, Del Frate, G, Pittino, M, De Anna, D, and Degrassi, A
- Published
- 2001
- Full Text
- View/download PDF
8. Deep retroperitoneal pelvic endometriosis: MR imaging appearance with laparoscopic correlation.
- Author
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Del Frate C, Girometti R, Pittino M, Del Frate G, Bazzocchi M, and Zuiani C
- Subjects
- Female, Humans, Practice Guidelines as Topic, Practice Patterns, Physicians', Statistics as Topic, Endometriosis pathology, Image Enhancement methods, Laparoscopy methods, Magnetic Resonance Imaging methods, Pelvic Inflammatory Disease diagnosis, Peritoneal Diseases diagnosis, Retroperitoneal Space pathology
- Abstract
Deep pelvic endometriosis is defined as subperitoneal infiltration of endometrial implants in the uterosacral ligaments, rectum, rectovaginal septum, vagina, or bladder. It is responsible for severe pelvic pain. Accurate preoperative assessment of disease extension is required for planning complete surgical excision, but such assessment is difficult with physical examination. Various sonographic approaches (transvaginal, transrectal, endoscopic transrectal) have been used for this purpose but do not allow panoramic evaluation. Furthermore, exploratory laparoscopy has limitations in demonstrating deep endometriotic lesions hidden by adhesions or located in the subperitoneal space. Despite some limitations, magnetic resonance (MR) imaging is able to directly demonstrate deep pelvic endometriosis. The MR imaging features depend on the type of lesions: infiltrating small implants, solid deep lesions mainly located in the posterior cul-de-sac and involving the uterosacral ligaments and torus uterinus, or visceral endometriosis involving the bladder and rectal wall. Solid deep lesions have low to intermediate signal intensity with punctate regions of high signal intensity on T1-weighted images, show uniform low signal intensity on T2-weighted images, and can demonstrate enhancement on contrast-enhanced images. MR imaging is a useful adjunct to physical examination and transvaginal or transrectal sonography in evaluation of patients with deep infiltrating endometriosis., (RSNA, 2006)
- Published
- 2006
- Full Text
- View/download PDF
9. A novel protocol that allows short-term stem cell expansion of both committed and pluripotent hematopoietic progenitor cells suitable for clinical use.
- Author
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Astori G, Malangone W, Adami V, Risso A, Dorotea L, Falasca E, Marini L, Spizzo R, Bigi L, Sala P, Tonutti E, Biffoni F, Rinaldi C, Del Frate G, Pittino M, and Degrassi A
- Subjects
- ADP-ribosyl Cyclase, ADP-ribosyl Cyclase 1, Antigens, CD34 analysis, Antigens, Differentiation analysis, Cell Differentiation, Cell Division drug effects, Drug Synergism, Erythropoietin pharmacology, Fetal Blood cytology, Flow Cytometry, Hematopoietic Stem Cells classification, Hematopoietic Stem Cells drug effects, Humans, Immunomagnetic Separation, Immunophenotyping, Infant, Newborn, Interleukin-3 pharmacology, Interleukin-6 pharmacology, Membrane Glycoproteins, Membrane Proteins pharmacology, NAD+ Nucleosidase analysis, Stem Cell Factor pharmacology, Thrombopoietin pharmacology, Antigens, CD, Cell Culture Techniques methods, Hematopoietic Stem Cell Transplantation methods, Hematopoietic Stem Cells cytology
- Abstract
To obtain long-term engraftment and hematopoiesis in myeloablated patients, the cell population used for hematopoietic reconstitution should include a sufficient number of early pluripotent hematopoietic stem cells (HSCs), along with committed cells from the various lineages. For this purpose, the small subset of CD34+ cells purified from different sources must be expanded ex vivo. Since cytokines may induce both proliferation and differentiation, expansion would provide a cell population comprising committed as well as uncommitted cells. Optimization of HSC expansion methods could be obtained by a combination of cytokines able to sustain renewal of pluripotent cells yet endowed with poor differentiation potential. We used variations of the combinations of cytokines described by Brugger et al. [W. Brugger, S. Heimfels, R. J. Berenson, R. Mertelsmann, and L. Kanz (1995) N. Engl. J. Med. 333, 283-287] and Piacibello et al. [W. Piacibello, F. Sanavio, L. Garetto, A. Severino, D. Bergandi, J. Ferrario, F. Fagioli, M. Berger, and M. Aglietta (1997) Blood 89, 2644-2653] to expand UCB CD34+ cells and monitored proliferation rate and phenotype after 14 days of culture. Several hematopoietic lineage-associated surface antigens were evaluated. Our data show that flt3L and thrombopoietin in combination with IL-3, while sustaining a high CD34+ proliferation rate, provide a relatively low enrichment in very early uncommitted CD34+/CD38- cells. Conversely, in the absence of IL-3, they are less effective in inducing proliferation yet significantly increase the number of CD34+/CD38- cells. A combination of the above protocols, applied simultaneously to aliquots of the same sample, would allow expansion of both committed and pluripotent HSC. This strategy may represent a significant improvement for clinical applications.
- Published
- 2001
- Full Text
- View/download PDF
10. [Total laparoscopic hysterectomy. Cost analysis].
- Author
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Pittino M, Facchin S, Rossi A, Zompicchiatti C, Del Frate G, and Zoletto M
- Subjects
- Costs and Cost Analysis, Female, Humans, Hysterectomy economics, Hysterectomy, Vaginal, Italy, Hysterectomy methods, Laparoscopy economics
- Abstract
Background: Hysterectomy performed totally by laparoscopy is still one of the most advanced procedure in endoscopic gynecological surgery. The aim of this study has been to evaluate the cost and the hospital charges of total laparoscopic hysterectomy as compared with those for total abdominal and vaginal hysterectomy., Methods: In particular, as far as laparoscopic technique is concerned, the cost of linear staplers have been analyzed as compared to disposable and non-disposable instruments for bipolar coagulation., Results: After analysing the data, it has been found that the use of linear staplers involves very high median costs of Lire 2,932,304 ($ 1,650) versus the use of non-disposable instruments of Lire 936,488 ($ 526). The median total hospital charges was of Lire 6,014,448 ($ 3,380) for abdominal hysterectomy, of Lire 4,449,617 ($ 2,500) for vaginal hysterectomy and of Lire 4,078,000 ($ 2,291) for laparoscopic technique with non-disposable supplies., Conclusions: The results confirm, on the one hand, that bipolar coagulation is a reliable method of hemostasis, and on the other hand that laparoscopic surgery is a technique that can be recommended as an alternative to laparotomy for hysterectomy with unquestionable benefits both for patients and hospital.
- Published
- 1999
11. Phenotypic characterization of immunomagnetically purified umbilical cord blood CD34+ cells.
- Author
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Belvedere O, Feruglio C, Malangone W, Bonora ML, Donini A, Dorotea L, Tonutti E, Rinaldi C, Pittino M, Baccarani M, Del Frate G, Biffoni F, Sala P, Hilbert DM, and Degrassi A
- Subjects
- Antigens, CD blood, Antigens, CD34 immunology, Cell Separation, Fetal Blood immunology, Flow Cytometry, Humans, Immunomagnetic Separation methods, Leukapheresis, Leukocytes, Mononuclear immunology, Phenotype, Stem Cells cytology, Stem Cells immunology, Antigens, CD34 blood, Fetal Blood cytology
- Abstract
This study describes the multilineage differentiation pattern of purified CD34+ stem cells obtained from human umbilical cord blood. CD34+ cells were collected from 49 umbilical cord blood samples. Following immunomagnetic purification, cells were double stained with anti CD34 and CD71, CD61, CD7, CD19, CD33, CD36 and triple stained with anti CD34, CD38 and HLA-DR. Analysis were performed using a FACScan flow cytometer. After purification, the mean CD34+ cells' purity was 85.49 +/- 7.08%. Several subpopulations of umbilical cord blood CD34+ cells were identified indicating different lineage commitment. The majority of CD34+ cells expressed both CD38 and HLA-DR (91.74 +/- 3.76%), while those lacking CD38 were 3.43 +/- 2.12% (CD38-DR+) and 1.81 +/- 1.54% (CD38-DR-). These data were compared to the expression of lineage commitment markers on purified CD34+ cells from 5 mobilized peripheral blood samples. The percentage of peripheral blood CD34+CD38-DR+) and CD34+CD38-DR- cells was significantly lower than umbilical cord blood, 0.24 +/- 0.18% and 0.04 +/- 0.03% respectively. The knowledge and standardized of umbilical cord blood CD34+ cells phenotype is critical since umbilical cord blood volume is limited. The homogeneity of CD34+ subpopulation phenotype suggests that monitoring of lineage differentiation antigens may not be relevant for clinical use of umbilical cord blood samples. However, the observed higher percentage of pluripotent CD34+38- stem cells in umbilical cord blood compared to peripheral blood, that might explain the successful clinical use of umbilical cord blood even when low number of cells are used, candidates these antigens as the predictive parameter for clinical use of umbilical cord blood samples.
- Published
- 1999
- Full Text
- View/download PDF
12. [Evaluation of complications caused by intrauterine devices].
- Author
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Fabiani G, Pittino M, D'Aietti V, Dalla Pria S, and Zaccagna G
- Subjects
- Female, Humans, Menorrhagia etiology, Pelvic Inflammatory Disease etiology, Pelvic Inflammatory Disease microbiology, Intrauterine Devices adverse effects
- Published
- 1987
13. [Cervical dysplasias. Comparative study of the cytological picture, anatomo-pathologic and age stage].
- Author
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Fabiani G, Pittino M, D'Aietti V, Dalla Pria S, and Zaccagna G
- Subjects
- Adult, Aged, Female, Humans, Middle Aged, Age Factors, Uterine Cervical Dysplasia pathology
- Published
- 1987
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