Search

Your search keyword '"Pitoulias, Matthaios G."' showing total 6 results
Start Over Author "Pitoulias, Matthaios G."
6 results on '"Pitoulias, Matthaios G."'

1. The link between central carbon metabolism and replication in Bacillus subtilis

Author

Pitoulias, Matthaios G.

Subjects
572, QR 75 Bacteria. Cyanobacteria
Abstract

It is a common observation that the growth rates of cells, specifically carbon metabolism is inextricably linked with replication. While progress has been made into both fields of metabolism and replication individually, the underlying mechanism demonstrating how these two pathways intercept is yet to be discovered. Recent genetic data from our collaborators indicate a coupling between replication and the lower part of glycolysis. Specifically, the spotlight is under the metabolic PykA/CcpN/YqfL cascade and the replication regime, referring to the helicase DnaC, the helicase loader DnaI, the primase DnaG and the polymerase DnaE. We tried to investigate the above hypothesis using biochemical and biophysical in vitro approaches such as Electrophoretic Mobility Shift Assays (EMSAs), Helicase Unwinding and Primer Extension assays, Mass Spectrometry and Surface Plasmon Resonance (SPR). Most importantly, our data revealed a functional interplay between the Pyruvate kinase PykA and the lagging strand polymerase DnaE through a series of primer extension assays. Additionaly, we discovered novel functional effects in vitro mediated by other metabolic proteins including PykA on the activities of the helicase DnaC, and also a reversal of the DnaG-mediated stimulation of the DnaC helicase activity.

Published
2020

2. The Long‐Term Progression of Aneurysmal Disease in Common Iliac Arteries After Standard EVAR and Its Clinical Implications.

Author

Pitoulias, Apostolos G., Chatzelas, Dimitrios, Pitoulias, Matthaios G., Politi, Loukia A., Christopoulos, Dimitrios C., Lazaridis, Ioannis, Saratzis, Nikolaos, Pitoulias, Georgios A., and Xu, Baohui

Abstract

Introduction: The progression of aneurysmal disease in the common iliac arteries (CIAs) after EVAR remains an insufficiently investigated field. The purpose of this study is to investigate the long‐term outcomes of standard elective EVAR with a variety of last‐generation bifurcated aortic endografts in relation with the progression of aneurysmal disease in the CIAs. Methods: This is a prospective cohort study of 168 patients, who were treated with six different endografts between 2013 and 2018 and completed the 5‐year computed tomography aortoangiography (CTA) follow‐up. Postoperative CTA analysis included CIA measurements at four diameters' points and two length levels in three postoperative time spots: first, 24th, and 60th months. All EVAR‐related adverse events were recorded, including migrations, endoleaks, limb occlusions, reinterventions, ruptures, and mortality. Results: At both time intervals, a significant and nearly linear dilatation and elongation of CIAs was evident. The mean percent increase, among all diameter points measured, was 11.7% at 24 months and 22.8% at 60 months (p < 0.001) with a nearly constant mean increase rate by 0.07 mm per month. The corresponding monthly elongation rate of total CIA length was 0.26 mm at 24 months and 0.34 mm at 5 years (p < 0.001). The respective monthly lengthening of CIAs' uncovered (from stent graft) segment was 0.10 and 0.15 mm, and the overall increase rate at 60 months was up to 53.9% (p < 0.001). A total of 20 EVAR‐related events were recorded, and multivariate analysis revealed that CIA dilatation served as a significant and independent predictor of long‐term EVAR failures, increasing the likelihood of adverse events by 2.8‐fold. Conclusions: Analysis of long‐term geometric CIA remodeling after a standard EVAR revealed a significant progression of aneurysmal disease in CIAs, which was associated with worsening EVAR outcomes and emphasizes the importance of a rigorous and extensive follow‐up protocol to maintain the long‐term EVAR effectiveness. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

5. Ultrasonographic hemodynamical and epidemiological factors in advancement of clinical manifestations in primary varicose veins

Author

Pitoulias, Apostolos G. Chatzelas, Dimitrios Kalogirou, Thomas E. Politi, Loukia A. Pitoulias, Matthaios G. Roussas, Nikolaos Kakisis, Ioannis Giannoukas, Athanasios D. and Pitoulias, Georgios A.

Abstract

Background: Primary varicose veins (PVV) represent the most prominent clinical manifestation of chronic venous disease (CVD) and has a complex pathophysiological background. The aim of our study was to investigate the impact of sonographic hemodynamical and contemporary epidemiological factors on the clinical severity of PVV. Methods: We analyzed the sonographic hemodynamical and clinical parameters from 159 consecutive CVD patients and 233 lower limbs with PVV of clinical stages C2, C3 and C4. Univariate and logistic regression analysis was performed between patients of C2 (N.=70 -30.0%) and C3 -4 stages and between subgroups C3 (N.=101 -43.3%) and C4 (N.=62 -26.6%). Results: Reflux of common femoral vein and saphenofemoral junction was detected in 43.3% and 65.7%. High venous reflux rates were found at the great saphenous above and below knee (90.1% -53.2%) and in Cockett perforators (80.5%). Logistic regression revealed that factors associated with the symptomatic C3; 4 stages were the duration of disease >10 years (P=0.015, insufficiency in two or more perforators (P

Published
2021

6. The link between central carbon metabolism and replication in bacillus subtilis

Author

Pitoulias, Matthaios G. and Pitoulias, Matthaios G.

Abstract

It is a common observation that the growth rates of cells, specifically carbon metabolism is inextricably linked with replication. While progress has been made into both fields of metabolism and replication individually, the underlying mechanism demonstrating how these two pathways intercept is yet to be discovered. Recent genetic data from our collaborators indicate a coupling between replication and the lower part of glycolysis. Specifically, the spotlight is under the metabolic PykA / CcpN / YqfL cascade and the replication regime, referring to the helicase DnaC, the helicase loader DnaI, the primase DnaG and the polymerase DnaE. We tried to investigate the above hypothesis using biochemical and biophysical in vitro approaches such as Electrophoretic Mobility Shift Assays (EMSAs), Helicase Unwinding and Primer Extension assays, Mass Spectrometry and Surface Plasmon Resonance (SPR). Most importantly, our data revealed a functional interplay between the Pyruvate kinase PykA and the lagging strand polymerase DnaE through a series of primer extension assays. Additionaly, we discovered novel functional effects in vitro mediated by other metabolic proteins including PykA on the activities of the helicase DnaC, and also a reversal of the DnaG-mediated stimulation of the DnaC helicase activity.

Catalog

Books, media, physical & digital resources
See catalog results