29 results on '"Pitaraki E"'
Search Results
2. Molecular identification of the primary cilium BBSome complex in pleural mesothelial cells
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Rouka, E, primary, Jagirdar, R, additional, Pitaraki, E, additional, Kotsiou, O, additional, Hatzoglou, C, additional, Gourgoulianis, K, additional, and Zarogiannis, S, additional
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- 2022
- Full Text
- View/download PDF
3. Human malignant pleural mesothelioma derived pleura fluid induces mesenchymal transition of benign pleural mesothelial cells
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Jagirdar, R, primary, Rouka, E, additional, Pitaraki, E, additional, Kotsiou, O, additional, Hatzoglou, C, additional, Gourgoulianis, K, additional, and Zarogiannis, S, additional
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- 2022
- Full Text
- View/download PDF
4. Dairy products, surrogate markers, and cardiovascular disease; a sex-specific analysis from the ATTICA prospective study
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Kouvari, M. Panagiotakos, D.B. Chrysohoou, C. Georgousopoulou, E.N. Yannakoulia, M. Tousoulis, D. Pitsavos, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Paliou, K. Metaxa, V. Skourlis, N. Papanikolaou, C. Kalogeropoulou, A. Pitaraki, E. Laskaris, A. Hatzigeorgiou, M. Grekas, A. Kokkou, E. Vassiliadou, C. Dedousis, G. Toutouza-Giotsa, M. Tselika, C. Poulopouloou, S. Toutouza, M. The ATTICA study Investigators
- Abstract
Background and aims: Dairy products are a very diverse food group with multiple effects on the cardiac health of men and women. The aim of this work was to evaluate the sex-specific association between dairy products (total and subtypes) and 10-year first fatal/nonfatal cardiovascular disease (CVD) incidence. Methods and results: In 2001–2002, n = 1514 men and n = 1528 women (>18 years old) from greater Athens area, Greece, were enrolled. Dietary assessment was based on a validated semi-quantitative food frequency questionnaire. Dairy product consumption was examined in relation to 10-year CVD incidence. Follow-up (2011–2012) was achieved in n = 2020 participants (n = 317 CVD cases). Ranking from lowest (2 servings/day) total dairy intake, CVD incidence in men was 17.8%, 15.0%, and 10.9% (p = 0.41), while in women it was 14%, 6.0%, and 5.7% (p = 0.02). Multiadjusted analysis revealed that total dairy intake protected against CVD only in women [Hazard Ratio (HR) = 0.48 and 95% Confidence Interval (95% CI) (0.23, 0.90)], irrespective of the fat content. Further analysis revealed that only fermented products (yogurt and cheese), protected against CVD. For per 200 g/day yogurt consumption, CVD risk was 20%–30% lower with this claim being more evident in women, while for per 30 g/day cheese intake, about 5% lower risk was observed particularly in men. As for butter, nonsignificant associations were highlighted. These associations were mainly retained in the case of hepatic steatosis, insulin resistance, and systemic inflammation. Conclusions: This work provides incentives for researchers to elucidate the diversity of ingredients and mechanisms through which dairy products exert their effect on cardiac health separately for men and women. © 2020 The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University
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- 2020
5. Dairy products, surrogate markers, and cardiovascular disease; a sex-specific analysis from the ATTICA prospective study
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Kouvari, Matina, primary, Panagiotakos, Demosthenes B., additional, Chrysohoou, Christina, additional, Georgousopoulou, Ekavi N., additional, Yannakoulia, Mary, additional, Tousoulis, Dimitrios, additional, Pitsavos, Christos, additional, Skoumas, Y., additional, Katinioti, N., additional, Papadimitriou, L., additional, Masoura, C., additional, Vellas, S., additional, Lentzas, Y., additional, Kambaxis, M., additional, Paliou, K., additional, Metaxa, V., additional, Skourlis, N., additional, Papanikolaou, C., additional, Kalogeropoulou, A., additional, Pitaraki, E., additional, Laskaris, A., additional, Hatzigeorgiou, M., additional, Grekas, A., additional, Kokkou, E., additional, Vassiliadou, C., additional, Dedousis, G., additional, Toutouza-Giotsa, M., additional, Tselika, C., additional, Poulopouloou, S., additional, and Toutouza, M., additional
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- 2020
- Full Text
- View/download PDF
6. Mediterranean diet and 10-year (2002-2012) incidence of diabetes and cardiovascular disease in participants with prediabetes: The ATTICA study
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Filippatos, T.D. Panagiotakos, D.B. Georgousopoulou, E.N. Pitaraki, E. Kouli, G.-M. Chrysohoou, C. Tousoulis, D. Stefanadis, C. Pitsavos, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Christou, A. Zana, A. Ntertimani, M. Kalogeropoulou, A. Pitaraki, E. Laskaris, A. Hatzigeorgiou, M. Grekas, A. Vassiliadou, C. Dedoussis, G. Toutouza-Giotsa, M. Tselika, C. Poulopoulou, S. Toutouza, M. The ATTICA Study Group
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BACKGROUND: Prediabetes has been related to an increased risk of developing diabetes and cardiovascular disease (CVD). AIM: The aim of the present study was to examine the effect of the Mediterranean diet on diabetes and CVD risk in subjects with impaired fasting glucose (IFG, i.e. fasting plasma glucose 100-125 mg/dl). METHODS: During 2001-2002, 3042 men and women (>18y) were enrolled for the study. The participants showed no clinical evidence of CVD or any other chronic disease, and were living in the greater Athens (Greece) area. In 2011 and 2012, the 10-year follow-up examinations were performed, including a working sample of n = 1875 participants without diabetes at baseline. Adherence to the Mediterranean diet at baseline evaluation was assessed using the MedDietScore (range 0-55). RESULTS: The prediabetic subjects (n = 343) had a significantly higher incidence of diabetes (25% vs. 10%, p < 0.001) and CVD (17.8% vs. 12.3%, p = 0.007) compared with subjects with normal glucose values. A significant trend towards lower diabetes and CVD incidence was observed with medium and high adherence to the Mediterranean diet compared with low adherence (p < 0.001). High adherence to the Mediterranean diet (>35/55 score) was associated with lower 10-year incidence of diabetes and CVD. In multiple logistic regression models, participants with high levels of adherence to the Mediterranean diet were significantly less affected by diabetes and CVD than those with low adherence levels. CONCLUSION: High adherence to the Mediterranean diet is associated with a low risk of developing diabetes and CVD in prediabetic subjects. © by Lab & Life Press/SBDR.
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- 2016
7. Adherence to Mediterranean diet and 10-year incidence (2002-2012) of diabetes: Correlations with inflammatory and oxidative stress biomarkers in the ATTICA cohort study
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Koloverou, E. Panagiotakos, D.B. Pitsavos, C. Chrysohoou, C. Georgousopoulou, E.N. Grekas, A. Christou, A. Chatzigeorgiou, M. Skoumas, I. Tousoulis, D. Stefanadis, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Christou, A. Zana, A. Ntertimani, M. Kalogeropoulou, A. Pitaraki, E. Laskaris, A. Hatzigeorgiou, M. The ATTICA Study Group
- Abstract
Background: The purpose of this work was to investigate the links between oxidative stress, inflammation and coagulation and their effect on Mediterranean diet-diabetes relationship. Methods: In 2001-2002, a random sample of 1514 men (18-87 years old) and 1528 women (18-89 years old) was selected to participate in the ATTICA study, where Athens is the major metropolis. A validated questionnaire was used to assess lifestyle and dietary factors. Adherence to Mediterranean diet was recorded using MedDietScore. Among others, oxidative stress and inflammatory biomarkers were recorded. During 2011-2012, the 10-year follow-up was performed. Diabetes incidence was defined according to the American Diabetes Association criteria. Results: A total of 191 incident cases of diabetes were documented, yielding an incidence of 12.9% (13.4% in men and 12.4% in women). Medium and high adherence was found to decrease diabetes risk by 49% (95% CI: 0.30, 0.88) and 62% (95% CI: 0.16, 0.88), respectively, compared with low adherence. A logarithmic trend between Mediterranean diet and diabetes incidence was also revealed (p for trend=0.042). Individuals with abnormal waist circumference (>94 for men, >80 for women) were benefited the most. Wholegrain cereals, fruits and legumes had the greatest predictive ability. The anti-diabetic effect of Mediterranean diet correlated with measurements of tumour necrosis factor-α, homocysteine and total antioxidant capacity. Conclusions: The reported results support the role of Mediterranean diet as a promising dietary tool for the primary prevention of diabetes, by attenuating inflammation and fostering total antioxidant capacity. This dietary pattern may have therapeutic potential for many cardiometabolic disorders associated with inflammation and/or oxidative stress. © 2016 John Wiley & Sons, Ltd.
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- 2016
8. Education status determines 10-year (2002-2012) survival from cardiovascular disease in Athens metropolitan area: The ATTICA study, Greece
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Panagiotakos, D.B. Georgousopoulou, E. Notara, V. Pitaraki, E. Kokkou, E. Chrysohoou, C. Skoumas, Y. Metaxa, V. Pitsavos, C. Stefanadis, C. ATTICA study group
- Abstract
Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality worldwide, and educational level seems to be an important determinant of the disease occurrence. The aim of this work was to investigate the association between education status and 10-year incidence of CVD, controlling for various socio-demographic lifestyle and clinical factors. From May 2001 to December 2002, 1514 men and 1528 women (>18 years) without any clinical evidence of CVD or any other chronic disease, at baseline, living in greater Athens area, Greece, were enrolled. In 2011-2012, the 10-year follow-up was performed in 2583 participants (15% of the participants were lost to follow-up). Incidence of fatal or non-fatal CVD was defined according to WHO-ICD-10 criteria. Education status was measured in years of schooling. The 10-year incidence of CVD was 15.7% [95% confidence intervals (CI) 14.1%-17.4%], 19.7% in men and 11.7% in women (Pgender < 0.001). Age-and gender-adjusted analyses revealed that those with low education (12 years of schooling). People in the low education group had higher prevalence of hypertension, diabetes and dyslipidaemias, were more likely to be smokers and sedentary, had less healthy dietary habits, as compared with those in the high education group. When controlling for participants' medical history, smoking, dietary and lifestyle habits, low education was no longer significantly associated with CVD, illustrating the mediating effect of clinical and behavioural factors in the link between education and disease. It was of interest that low education status interacted with alcohol drinking, enhancing the adverse effect of low education on CVD risk (relative risk 1.44, 95% CI 0.94%-2.20%), after various adjustments made. In this study, it was concluded that low educational level was associated with increased CVD risk. This was mainly explained by the intermediate association of low education with unhealthy choices that consequently worsen clinical status. © 2016 John Wiley & Sons Ltd.
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- 2016
9. Metabolic syndrome and 10-year cardiovascular disease incidence: The ATTICA study
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Kastorini, C.-M. Panagiotakos, D.B. Georgousopoulou, E.N. Laskaris, A. Skourlis, N. Zana, A. Chatzinikolaou, C. Chrysohoou, C. Puddu, P.E. Tousoulis, D. Stefanadis, C. Pitsavos, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Christou, A. Zana, A. Ntertimani, M. Kalogeropoulou, A. Pitaraki, E. Laskaris, A. Mihail Hatzigeorgiou Athanasios Grekas Kokkou, E. ATTICA Study Group
- Abstract
Aims: To evaluate the influence of metabolic syndrome (MetS) as well as inflammatory and renal markers on cardiovascular disease (CVD) incidence. Methods and results: During 2001-2002, 1514 men and 1528 women (>18y) without any clinical evidence of CVD or any other chronic disease, at baseline, living in greater Athens area, Greece, were enrolled. In 2011-2012, the 10-year follow-up was performed in 2583 participants (15% of the participants were lost to follow-up). Incidence of fatal or non-fatal CVD was defined according to WHO-ICD-10 criteria. MetS was defined using three definitions, provided by the National Cholesterol Education Program Adult Treatment panel III (revised NCEP ATP III), the International Diabetes Federation (IDF) or the Harmonized definition. Furthermore, the contributory predictive role of C-reactive protein (CRP), inteleukin-6, uric acid and estimated glomerular filtration rate in the aforementioned models was evaluated. History of MetS-NCEP was positively associated with CVD, adjusting for potential confounding factors (OR:1.83, 95%CI:1.24-2.72). Not statistically significant associations with CVD incidence were observed when using the IDF or the Harmonized definition. Additionally, none of the added inflammatory and renal function markers mediated the influence of MetS on CVD incidence (all p's from Sobel test >0.40). C-statistic values for the MetS definitions used exceeded 0.789 (CI:0.751-0.827), indicating fair-to-good predictive probability of the models. Conclusion: Results of the present work revealed the negative impact of MetS-NCEP, but not of the other MetS definitions, on CVD incidence, a key-point that may help in better understanding the role of IDF and Harmonized MetS definitions on CVD. © 2015 The Italian Society of Diabetology, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition, and the Department of Clinical Medicine and Surgery, Federico II University.
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- 2016
10. Metabolic syndrome, adherence to the Mediterranean diet and 10-year cardiovascular disease incidence: The ATTICA study
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Kastorini, C.-M. Panagiotakos, D.B. Chrysohoou, C. Georgousopoulou, E. Pitaraki, E. Puddu, P.E. Tousoulis, D. Stefanadis, C. Pitsavos, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Christou, A. Zana, A. Ntertimani, M. Kalogeropoulou, A. Laskaris, A. Hatzigeorgiou, M. Grekas, A. Kokkou, E. Tsetsekou, E. Vassiliadou, C. Dedoussis, G. Toutouza-Giotsa, M. Tselika, C. Poulopoulou, S. Toutouza, M. The ATTICA Study Group
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Background and aims: To better understand the metabolic syndrome (MS) spectrum through principal components analysis and further evaluate the role of the Mediterranean diet on MS presence. Methods: During 2001-2002, 1514 men and 1528 women (>18y) without any clinical evidence of CVD or any other chronic disease, at baseline, living in greater Athens area, Greece, were enrolled. In 2011-2012, the 10-year follow-up was performed in 2583 participants (15% of the participants were lost to follow-up). Incidence of fatal or non-fatal CVD was defined according to WHO-ICD-10 criteria. MS was defined by the National Cholesterol Education Program Adult Treatment panel III (revised NCEP ATP III) definition. Adherence to the Mediterranean diet was assessed using the MedDietScore (range 0-55). Results: Five principal components were derived, explaining 73.8% of the total variation, characterized by the: a) body weight and lipid profile, b) blood pressure, c) lipid profile, d) glucose profile, e) inflammatory factors. All components were associated with higher likelihood of CVD incidence. After adjusting for various potential confounding factors, adherence to the Mediterranean dietary pattern for each 10% increase in the MedDietScore, was associated with 15% lower odds of CVD incidence (95%CI: 0.71-1.06). For the participants with low adherence to the Mediterranean diet all five components were significantly associated with increased likelihood of CVD incidence. However, for the ones following closely the Mediterranean pattern positive, yet not significant associations were observed. Conclusion: Results of the present work propose a wider MS definition, while highlighting the beneficial role of the Mediterranean dietary pattern. © 2015 Elsevier Ireland Ltd.
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- 2016
11. Dietary patterns and 10-year (2002-2012) incidence of type 2 diabetes: Results from the ATTICA cohort study
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Koloverou, E. Panagiotakos, D.B. Georgousopoulou, E.N. Grekas, A. Christou, A. Chatzigeorgiou, M. Chrysohoou, C. Tousoulis, D. Stefanadis, C. Pitsavos, C. ATTICA Study Group Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Christou, A. Zana, A. Ntertimani, M. Kalogeropoulou, A. Pitaraki, E. Laskaris, A. Hatzigeorgiou, M. Grekas, A. Vassiliadou, C. Dedoussis, G. Toutouza-Giotsa, M. Tselika, C. Poulopoulou, S. Toutouza, M.
- Abstract
AIM: To identify dietary patterns among apparently healthy individuals and to determine their long-term effect on diabetes incidence. METHODS: During 2001-2002, a random sample of 3,042 men and women (18-89 years old), living in greater Athens, was randomly selected to participate in the study. During 2011-2012, the 10-year follow-up was performed in 2,583 participants (15% drop-out rate). After excluding participants with diabetes at baseline and those for whom no information on diabetes status was available at follow- up, the working sample consisted of 1,485 participants. Dietary habits were assessed by means of a validated semiquantitative, food frequency questionnaire. Factor analysis was performed to extract dietary patterns from 18 food groups. RESULTS: Diabetes diagnosis at follow-up was made in 191 participants, yielding an incidence rate of 12.9%. Six factors (i.e. dietary patterns) were identified that explained 54% of the variation in consumption. After adjusting for major confounders, and stratification by age-group, logistic regression revealed that the most healthful pattern consisted of the consumption of fruits, vegetables, legumes, bread, rusk, and pasta which reduced the 10-year diabetes risk by 40%, among participants aged 45-55 years. The association reached marginal statistical significance (95% CI: 0.34, 1.07), while no significant association was observed for the other age-groups. When the analysis was additionally adjusted for carbohydrate percentage, statistical significance was lost completely, suggesting a possibly mediating effect of this macronutrient. CONCLUSIONS: The results confirm the potentially protective effect of a plant-based dietary pattern in the primary prevention of diabetes, in particular among middle-aged people. Carbohydrate content may be a specific factor in this relationship; other micronutrients found in plant-based food groups may also play a role. © by Lab & Life Press/SBDR.
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- 2016
12. Adherence to mediterranean diet offers an additive protection over the use of statin therapy: Results from the ATTICA study (2002-2012)
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Panagiotakos, D.B. Georgousopoulou, E.N. Georgiopoulos, G.A. Pitsavos, C. Chrysohoou, C. Skoumas, I. Ntertimani, M. Laskaris, A. Papadimitriou, L. Tousoulis, D. Stefanadis, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Christou, A. Zana, A. Ntertimani, M. Kalogeropoulou, A. Pitaraki, E. Laskaris, A. Hatzigeorgiou, M. Vassiliadou, C. Dedoussis, G. Toutouza-Giotsa, M. Tselika, C. Poulopoulou, S. Toutouza, M. ATTICA Study group
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cardiovascular diseases - Abstract
Background and Aims: The protective role of Mediterranean diet on cardiovascular disease (CVD) risk has been extensively discussed in the literature, but its incremental effect over the use of CVD risk reducing agents (such as hypolipidemic treatment) has rarely been evaluated. Methods: The ATTICA study was carried out in the Athens area during 2001-2002 and included 3042 participants free of CVD at baseline (49.8% men, aged 18-89 years). Adherence to Mediterranean diet was assessed using the MedDietScore (range 0-55) and statin use was recorded for all subjects. During 2011-2012, 2583 out of the 3042 baseline participants attended the 10-year follow-up of the ATTICA study (15% lost-to-follow-up) and CVD development was recorded. Results: Adherence to Mediterranean diet (highest tertile) decreased CVD risk by 29.3% (Hazard Ratio (HR): 0.707, 95% Confidence Intervals (CI): 0.537-0.831) as compared with the lowest tertile, independently of statin use. Patients with hyperlipidemia on a statin that adopted unhealthy dietary habits (lowest tertile) had 75% increased CVD risk than normolipidemic subjects with healthy dietary habits (HR=1.75, 95%CI: 1.33-2.29). The addition of Mediterranean diet tertiles in the multivariable model reclassified 46.7% of the participants to CVD risk categories. Conclusion: Adherence to Mediterranean diet confers a considerable reduction in CVD risk, independently of gender, age, family history of CVD, diabetes mellitus, smoking status, hypertension and physical activity status. Therefore, CVD prevention strategies should involve the implementation of a Mediterranean diet in both the general population and patients on a statin. © 2015 Bentham Science Publishers.
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- 2015
13. Effects of alcohol consumption and the metabolic syndrome on 10-year incidence of diabetes: The ATTICA study
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Koloverou, E. Panagiotakos, D.B. Pitsavos, C. Chrysohoou, C. Georgousopoulou, E.N. Metaxa, V. Stefanadis, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, S. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Christou, A. Zana, A. Ntertimani, M. Kalogeropoulou, A. Pitaraki, E. Laskaris, A. Hatzigeorgiou, M. Grekas, A. Vassiliadou, C. Dedoussis, G. Toutouza-Giotsa, M. Tselika, C. Poulopoulou, S. Toutouza, M. the ATTICA study group
- Abstract
Aim: The purpose of this prospective study was to investigate the effect of alcohol consumption on the 10-year diabetes incidence. Methods: In 2001-2002, a random sample of 1514 men (18-89 years old) and 1528 women (18-87 years old) was selected to participate in the ATTICA study (Athens metropolitan area, Greece). Among various other characteristics, average daily alcohol intakes (abstention, low, moderate, high) and type of alcoholic drink were evaluated. Diabetes was defined according to American Diabetes Association criteria. During 2011-2012, the 10-year follow-up was performed. Results: The 10-year incidence of diabetes was 13.4% in men and 12.4% in women. After making various adjustments, those who consumed up to 1 glass/day of alcohol had a 53% lower diabetes risk (RR = 0.47; 95% CI: 0.26, 0.83) compared with abstainers, while trend analysis revealed a significant U-shaped relationship between quantity of alcohol drunk and diabetes incidence (P< 0.001 for trend). Specific types of drinks were not associated with diabetes incidence; however, a one-unit increase in ratio of wine/beer/vodka vs. other spirits was associated with an 89% lower risk of diabetes (RR = 0.11; 95% CI: 0.02, 0.67). The protective effect of low alcohol consumption on diabetes incidence was more prominent among individuals with stricter adherence to the Mediterranean diet (RR = 0.08; 95% CI: 0.011, 0.70) and without the metabolic syndrome (RR = 0.34; 95% CI: 0.16, 0.70). Conclusion: This work revealed the protective effect of modest alcohol consumption of particularly wine and beer against the long-term incidence of diabetes, possibly due to their pleiotropic health effects. © 2014 Elsevier Masson SAS.
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- 2015
14. Exploring the path of Mediterranean diet on 10-year incidence ofcardiovascular disease: The ATTICA study (2002-2012)
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Panagiotakos, D.B. Georgousopoulou, E.N. Pitsavos, C. Chrysohoou, C. Skoumas, I. Pitaraki, E. Georgiopoulos, G.A. Ntertimani, M. Christou, A. Stefanadis, C. Skoumas, Y. Katinioti, N. Papadimitriou, L. Masoura, C. Vellas, S. Lentzas, Y. Kambaxis, M. Palliou, K. Metaxa, V. Ntzouvani, A. Mpougatsas, D. Skourlis, N. Papanikolaou, C. Kouli, G.-M. Zana, A. Kalogeropoulou, A. Laskaris, A. Hatzigeorgiou, M. Grekas, A. Vassiliadou, C. Dedoussis, G. Toutouza-Giotsa, M. Tselika, C. Poulopoulou, S. Toutouza, M. The ATTICA Study Group
- Abstract
Background and aims: A Mediterranean diet has been associated with lower all-cause and cardiovascular disease (CVD) morbidity and mortality, but the clinical and behavioral pathway has not been well understood and appreciated. The aim of this work was to explore the path between adherence to a Mediterranean-type diet, lifestyle behaviors, clinical status, and a 10-year incidence of CVD. Methods and results: The ATTICA study was carried out in the Athens area during 2001-2002 and included 3042 participants free of CVD at baseline (49.8% men, aged 18-89). Adherence to a Mediterranean diet was assessed using the MedDietScore (range 0-55). During 2011-2012, 2583 out of the 3042 participants were found during the 10-year follow-up (15% lost to follow-up). Adherence to a Mediterranean diet decreased CVD risk (relative Risk (RR) per 1/55 unit = 0.96, 95% confidence interval (CI): 0.93, 1.00), independently of various sociodemographic, lifestyle, and clinical factors. Subgroup analyses revealed that participants with an unhealthy lifestyle (i.e., smokers, and obese and sedentary persons) remained protected from CVD through a greater adherence to a Mediterranean diet (RR for smokers = 0.92, 95%CI: 0.88, 0.97; RR for obese participants = 0.90, 95%CI: 0.82, 0.979; and RR for sedentary participants = 0.95, 95%CI: 0.90, 0.99). Path analysis revealed that adherence to a Mediterranean diet not only decreases the levels of C-reactive protein and interleukin-6 but also has an independent protective role against CVD risk per se (total effect of the MedDietScore on CVD = -0.003, 95%CI: -0.005 to 0.000). Conclusion: Adherence to a Mediterranean diet confers a considerable reduction on CVD risk, independent of various factors. Therefore, even subjects with unhealthy lifestyle behaviors may benefit from adherence to this diet, suggesting another dimension to prevention strategies. © 2014 Elsevier B.V.
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- 2015
15. Exploring the path of Mediterranean diet on 10-year incidence of cardiovascular disease: The ATTICA study (2002–2012)
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Panagiotakos, D.B., primary, Georgousopoulou, E.N., additional, Pitsavos, C., additional, Chrysohoou, C., additional, Skoumas, I., additional, Pitaraki, E., additional, Georgiopoulos, G.A., additional, Ntertimani, M., additional, Christou, A., additional, and Stefanadis, C., additional
- Published
- 2015
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16. Differential effects of biocompatible peritoneal dialysis fluids on human mesothelial and endothelial cells in 2D and 3D phenotypes.
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Jagirdar RM, Pitaraki E, Rouka E, Papazoglou ED, Bartosova M, Zebekakis P, Schmitt CP, Zarogiannis SG, and Liakopoulos V
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- Humans, Icodextrin metabolism, Icodextrin pharmacology, Dialysis Solutions adverse effects, Dialysis Solutions metabolism, Peritoneum metabolism, Phenotype, Amino Acids metabolism, Amino Acids pharmacology, Glucose pharmacology, Glucose metabolism, Cells, Cultured, Epithelial Cells, Endothelial Cells, Peritoneal Dialysis
- Abstract
Introduction: Peritoneal dialysis (PD) is a life maintaining treatment in patients with end-stage renal disease. Its chronic application leads to peritoneal mesothelial layer denudation and fibrotic transformation along with vascular activation of inflammatory pathways. The impact of different PD fluids (PDF) on mesothelial and endothelial cell function and repair mechanisms are not comprehensively described., Materials and Methods: Mesothelial (MeT-5A) and endothelial cells (EA.hy926) were cultured in 1:1 ratio with cell medium and different PDF (icodextrin-based, amino acid-based, and glucose-based). Cell adhesion, cell migration, and cell proliferation in 2D and spheroid formation and collagen gel contraction assays in 3D cell cultures were performed., Results: Cell proliferation and cell-mediated gel contraction were both significantly decreased in all conditions. 3D spheroid formation was significantly reduced with icodextrin and amino acid PDF, but unchanged with glucose PDF. Adhesion was significantly increased by amino acid PDF in mesothelial cells and decreased by icodextrin and amino acid PDF in endothelial cells. Migration capacity was significantly decreased in mesothelial cells by all three PDF, while endothelial cells remained unaffected., Conclusions: In 3D phenotypes the effects of PDF are more uniform in both mesothelial and endothelial cells, mitigating spheroid formation and gel contraction. On the contrary, effects on 2D phenotypes are more uniform in the icodextrin and amino acid PDF as opposed to glucose ones and affect mesothelial cells more variably. 2D and 3D comparative assessments of PDF effects on the main peritoneal membrane cell barriers, the mesothelial and endothelial, could provide useful translational information for PD studies., (© 2023 The Authors. Artificial Organs published by International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC.)
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- 2024
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17. 2-Deoxy-glucose ameliorates the peritoneal mesothelial and endothelial barrier function perturbation occurring due to Peritoneal Dialysis fluids exposure.
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Pitaraki E, Jagirdar RM, Rouka E, Bartosova M, Sinis SI, Gourgoulianis KI, Eleftheriadis T, Stefanidis I, Liakopoulos V, Hatzoglou C, Schmitt CP, and Zarogiannis SG
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- Humans, Sodium-Glucose Transporter 2 metabolism, Deoxyglucose pharmacology, Deoxyglucose metabolism, Endothelial Cells, Peritoneum pathology, Dialysis Solutions metabolism, Dialysis Solutions pharmacology, Glucose metabolism, Epithelial Cells metabolism, Cells, Cultured, Peritoneal Dialysis adverse effects, Peritoneal Fibrosis metabolism
- Abstract
Peritoneal dialysis (PD) and prolonged exposure to PD fluids (PDF) induce peritoneal membrane (PM) fibrosis and hypervascularity, leading to functional PM degeneration. 2-deoxy-glucose (2-DG) has shown potential as PM antifibrotic by inhibiting hyper-glycolysis induced mesothelial-to-mesenchymal transition (MMT). We investigated whether administration of 2-DG with several PDF affects the permeability of mesothelial and endothelial barrier of the PM. The antifibrotic effect of 2-DG was confirmed by the gel contraction assay with embedded mesothelial (MeT-5A) or endothelial (EA.hy926) cells cultured in Dianeal® 2.5 % (CPDF), BicaVera® 2.3 % (BPDF), Balance® 2.3 % (LPDF) with/without 2-DG addition (0.2 mM), and qPCR for αSMA, CDH2 genes. Moreover, 2-DG effect was tested on the permeability of monolayers of mesothelial and endothelial cells by monitoring the transmembrane resistance (R
TM ), FITC-dextran (10, 70 kDa) diffusion and mRNA expression levels of CLDN-1 to -5, ZO1, SGLT1, and SGLT2 genes. Contractility of MeT-5A cells in CPDF/2-DG was decreased, accompanied by αSMA (0.17 ± 0.03) and CDH2 (2.92 ± 0.29) gene expression fold changes. Changes in αSMA, CDH2 were found in EA.hy926 cells, though αSMA also decreased under LPDF/2-DG incubation (0.42 ± 0.02). Overall, 2-DG mitigated the PDF-induced alterations in mesothelial and endothelial barrier function as shown by RTM , dextran transport and expression levels of the CLDN-1 to -5, ZO1, and SGLT2. Thus, supplementation of PDF with 2-DG not only reduces MMT but also improves functional permeability characteristics of the PM mesothelial and endothelial barrier., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2023 Elsevier Inc. All rights reserved.)- Published
- 2024
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18. The effect of cigarette smoke extract exposure on the size and sexual behaviour of Drosophila melanogaster.
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Giannopoulos AS, Giannakou L, Gourgoulianni N, Pitaraki E, Jagirdar R, Marnas P, Tzamalas PI, Rouka E, Livanou E, Hatzoglou C, Gourgoulianis K, Lüpold S, Blanckenhorn WU, and Zarogiannis SG
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- Animals, Humans, Male, Female, Sexual Behavior, Animal, Copulation, Courtship, Drosophila melanogaster, Cigarette Smoking
- Abstract
Drosophila melanogaster is a widely used animal model in human diseases and to date it has not been applied to the study of the impact of tobacco use on human sexual function. Hence, this report examines the effects of different concentrations of cigarette smoke extract (CSE) exposure on the size and sexual behavior of D. melanogaster. Wild-type flies were held in vials containing CSE-infused culture media at concentrations of 10%, 25%, and 50% for three days, and their offspring were reared under the same conditions before measuring their body size and mating behavior. CSE exposure during development reduced the tibia length and body mass of emerging adult flies and prolonged the time required for successful courtship copulation success, while courtship behaviors (wing extension, tapping, abdomen bending, attempted copulation) remained largely unchanged. Our findings indicate that CSE exposure negatively affects the development of flies and their subsequent reproductive success. Future experiments should investigate the CSE effect on male female fertility., Competing Interests: Declaration of Competing Interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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19. Changes in expression of mesothelial BBS genes in 2D and 3D after lithium chloride and ammonium sulphate induction of primary cilium disturbance: a pilot study.
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Rouka E, Jagirdar RM, Sarrigeorgiou I, Pitaraki E, Sinis SI, Varsamas C, Papazoglou ED, Kotsiou OS, Lymberi P, Giannou A, Hatzoglou C, Gourgoulianis KI, and Zarogiannis SG
- Abstract
Background: Malignant pleural mesothelioma (MPM), a rare and aggressive pleural tumor, has significant histological and molecular heterogeneity. Primary Cilium (PC), an organelle of emerging importance in malignancies, has been scarcely investigated in MPM. A critical molecular complex for the PC function is the BBSome and here we aimed at assessing its expression patterns in ordinary 2D and spheroid 3D cell cultures., Methods: A human benign mesothelial cell line (MeT-5A), MPM cell lines (M14K, epithelioid MPM; MSTO, biphasic MPM), and primary MPM cells (pMPM) were used. Primers specific for the human BBS1, 2, 4, 5, 7, 9, 18 transcripts were designed, and quantitative real-time PCR (qRT-PCR) was done with β-actin as the gene of reference. The relative gene expression across 2D and 3D cultures was analyzed by the expression factor (mean of 1/ΔCt values). With the 2
-∆∆Ct method the gene expression fold changes were assessed from qRT-PCR data. Molecular changes using the PC-modulating drugs ammonium sulfate (AS) and lithium chloride (LC) were also determined., Results: PC was present in all cells used in the study at approximately 15% of the observed area. BBSome transcripts were differentially expressed in different dimensions of cell culture (2D vs. 3D) in all cell lines and pMPM. Treatment with AS and LC affected the expression of the ciliary BBS2 and BBS18 genes in the benign as well as in the MPM cells., Conclusions: These data indicate distinct BBSome molecular profiles in human benign and MPM cells cultured in 2D and 3D dimensions and support the notion that PC genes should be investigated as potential MPM therapeutic targets., (© 2023. The Author(s).)- Published
- 2023
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20. Effects of pharmacological primary cilium disturbance in the context of in vitro 2D and 3D malignant pleura mesothelioma.
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Jagirdar RM, Pitaraki E, Kotsiou OS, Rouka E, Sinis SI, Varsamas C, Marnas P, Stergiopoulou E, Giannou A, Hatzoglou C, Gourgoulianis KI, and Zarogiannis SG
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- Animals, Pleura metabolism, Pleura pathology, Cilia metabolism, Cell Line, Tumor, Mammals, Mesothelioma, Malignant pathology, Mesothelioma metabolism, Pleural Neoplasms metabolism, Lung Neoplasms pathology
- Abstract
Introduction: Primary cilium (PC) is a single non-motile antenna-like organelle composed of a microtubule core axon originating from the mother centriole of the centrosome. The PC is universal in all mammalian cells and protrudes to the extracellular environment receiving mechanochemical cues that it transmits in the cell., Aim: To investigate the role of PC in mesothelial malignancy in the context of two-dimensional (2D) and three-dimensional (3D) phenotypes., Materials and Methods: The effect of pharmacological deciliation [using ammonium sulphate (AS) or chloral hydrate (CH)] and PC elongation [using lithium chloride (LC)] on cell viability, adhesion, and migration (2D cultures) as well as in mesothelial sphere formation, spheroid invasion and collagen gel contraction (3D cultures) was investigated in benign mesothelial MeT-5A cells and in malignant pleural mesothelioma (MPM) cell lines, M14K (epithelioid) and MSTO (biphasic), and primary malignant pleural mesothelioma cells (pMPM)., Results: Pharmacological deciliation or elongation of the PC significantly affected cell viability, adhesion, migration, spheroid formation, spheroid invasion and collagen gel contraction in MeT-5A, M14K, MSTO cell lines and in pMPM cells compared to controls (no drug treatment)., Conclusions: Our findings indicate a pivotal role of the PC in functional phenotypes of benign mesothelial cells and MPM cells., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2023 Elsevier Inc. All rights reserved.)
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- 2023
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21. Correction to: Evaluation of AQP4/TRPV4 Channel Co-expression, Microvessel Density, and its Association with Peritumoral Brain Edema in Intracranial Meningiomas.
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Faropoulos K, Polia A, Tsakona C, Pitaraki E, Moutafidi A, Gatzounis G, and Assimakopoulou M
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- 2022
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22. Evaluation of AQP4/TRPV4 Channel Co-expression, Microvessel Density, and its Association with Peritumoral Brain Edema in Intracranial Meningiomas.
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Faropoulos K, Polia A, Tsakona C, Pitaraki E, Moutafidi A, Gatzounis G, and Assimakopoulou M
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- Adult, Aged, Aged, 80 and over, Aquaporin 4 metabolism, Brain blood supply, Brain Edema etiology, Brain Edema genetics, Brain Edema pathology, Female, Humans, Male, Meningeal Neoplasms complications, Meningeal Neoplasms genetics, Meningeal Neoplasms pathology, Meningioma complications, Meningioma genetics, Meningioma pathology, Microvessels pathology, Middle Aged, RNA, Messenger genetics, RNA, Messenger metabolism, TRPV Cation Channels metabolism, Aquaporin 4 genetics, Brain Edema metabolism, Meningeal Neoplasms metabolism, Meningioma metabolism, TRPV Cation Channels genetics
- Abstract
Apart from VEGF-A pathway activation, the existence of peritumoral edema (PTBE) in meningiomas has been correlated with the expression levels of water transporter aquaporin 4 (AQP4). A novel cooperation of AQP4 with the transient receptor potential isoform 4 (TRPV4), a polymodal swelling-sensitive cation channel, has been proposed for regulating cell volume in glial cells. We investigated AQP4/TRPV4 channel co-expression in meningiomas along with the neovascularization of tumors and associate with PTBE. Immunohistochemical staining for AQP4 and TRPV4 expression was quantitatively analyzed in semi-serial sections of archival tissue from 174 patients. Microvessel density was expressed as microvessel count (MVC). PTBE was measured and edema index (EI) was assessed in 23 patients, based on magnetic resonance images (MRI) whereas mRNA levels of AQP4 and TRPV4 were evaluated in these patients using quantitative real-time PCR. High AQP4 was associated with lower-tumor grade (p < 0.05). AQP4 and TRPV4 were correlated in benign (WHO, grade I) (p < 0.0001) but not in high-grade (WHO, grades II and III) meningiomas (p > 0.05). AQP4/TRPV4 levels were independent of EI and MVC (p > 0.05). In contrast, EI was correlated to MVC (p = 0.02). AQP4/TRPV4 co-expression was detected in both edematous and non-edematous meningiomas. However, most of tumors with larger edema (EI ≥ 2) demonstrated increased levels of AQP4 and TRPV4. Importantly, peri-meningioma tissue of edematous meningiomas demonstrated significantly increased expression for AQP4 (p = 0.007) but not for TRPV4 (p > 0.05) compared with the main tumor. AQP4 and TRPV4 expression is rather associated with a response to vasogenic edema of meningiomas than with edema formation., (© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature.)
- Published
- 2021
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23. Cell and extracellular matrix interaction models in benign mesothelial and malignant pleural mesothelioma cells in 2D and 3D in-vitro.
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Jagirdar RM, Papazoglou ED, Pitaraki E, Kouliou OA, Rouka E, Giannakou L, Giannopoulos S, Sinis SI, Hatzoglou C, Gourgoulianis KI, and Zarogiannis SG
- Abstract
Malignant pleural mesothelioma (MPM) is an aggressive tumour that grows in the pleural cavity. MPM spheroids released in the pleural fluid can form new tumour foci. Cell-cell, cell-extracellular matrix (ECM) interactions in 2D and 3D impact malignant cell behaviour during cell adhesion, migration, proliferation and epithelial-mesenchymal transition (EMT). In this study, epithelioid, biphasic and sarcomatoid MPM cell types as well as benign mesothelial cells were tested with regards to the above phenotypes. Fibronectin (FN) and homologous cell-derived extracellular matrix (hcd-ECM) treated substratum differentially affected the above phenotypes. 3D MPM spheroid invasion was higher in FN-collagen matrices in the epithelioid and biphasic cells, while 3D cell cultures of epithelioid and sarcomatoid MPM cells in FN-collagen showed a higher contractility compared to hcd-ECM-collagen. Cell aggregates demonstrated invasive behaviour in hcd-ECM matrices alone. Our results suggest that ECM and the dimensionality affect malignant cell behaviour during cell culture studies., (© 2020 John Wiley & Sons Australia, Ltd.)
- Published
- 2021
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24. In Vitro Characterization of Cisplatin and Pemetrexed Effects in Malignant Pleural Mesothelioma 3D Culture Phenotypes.
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Papazoglou ED, Jagirdar RM, Kouliou OA, Pitaraki E, Hatzoglou C, Gourgoulianis KI, and Zarogiannis SG
- Abstract
Malignant pleural mesothelioma (MPM) is an aggressive cancer with poor prognosis. The main treatment for MPM is doublet chemotherapy with Cisplatin and Pemetrexed, while ongoing trials test the efficacy of pemetrexed monotherapy. However, there is lack of evidence regarding the effects of Cisplatin and Pemetrexed on MPM cell phenotypes, especially in three-dimensional (3D) cell cultures. In this study, we evaluated the effects Cisplatin and Pemetrexed on cell viability using homologous cell derived extracellular matrix (hECM) as substratum and subsequently in the following 3D cell culture phenotypes: tumor spheroid formation, tumor spheroid invasion, and collagen gel contraction. We used benign mesothelial MeT-5A cells as controls and the MPM cell lines M14K (epithelioid), MSTO (biphasic), and ZL34 (sarcomatoid). Cell viability of all cell lines was significantly decreased with all treatments. Mean tumor spheroid perimeter was reduced after treatment with Pemetrexed or the doublet therapy in all cell lines, while Cisplatin reduced the mean spheroid perimeter of MeT-5A and MSTO cells. Doublet treatment reduced the invasive capacity of spheroids of cell lines into collagenous matrices, while Cisplatin lowered the invasion of the MSTO and ZL34 cell lines, and Pemetrexed lowered the invasion of MeT-5A and ZL34 cell lines. Treatment with Pemetrexed or the combination significantly reduced the collagen gel contraction of all cell lines, while Cisplatin treatment affected only the MeT-5A and M14K cells. The results of the current study can be used as an in vitro 3D platform for testing novel drugs against MPM for ameliorating the effects of first line chemotherapeutics.
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- 2019
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25. 2-Deoxy-glucose Enhances the Effect of Cisplatin and Pemetrexed in Reducing Malignant Pleural Mesothelioma Cell Proliferation But Not Spheroid Growth.
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Gerogianni I, Pitaraki E, Jagirdar RM, Kouliou O, Giannakou L, Giannopoulos S, Papazoglou E, Hatzoglou C, Gourgoulianis KI, and Zarogiannis SG
- Subjects
- Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Humans, Mesothelioma, Malignant, Antineoplastic Agents pharmacology, Cisplatin pharmacology, Deoxyglucose pharmacology, Lung Neoplasms drug therapy, Mesothelioma drug therapy, Pemetrexed pharmacology, Pleural Neoplasms drug therapy, Spheroids, Cellular drug effects
- Abstract
Background/aim: Malignant pleural mesothelioma (MPM) is a therapy-resistant neoplasm of the pleura. Standard chemotherapy consists of a combination of cisplatin (CPDD) and pemetrexed (PEM). The aim of this study was to assess whether inhibition of aerobic glycolysis by 2-deoxy-glucose (2DG) would enhance the effects of standard chemotherapy., Materials and Methods: MeT-5A, M14K, MSTO and ZL34 cell lines were used. Cell viability with 2DG and cell proliferation and spheroid formation with CPDD+PEM alone and with 2-DG were tested., Results: Viability with 2-DG was dose-dependent. Cell proliferation with CPDD+PEM on 2D surface was reduced in all cell types, 2-DG inclusion demonstrated a synergistic effect in MSTO and ZL34 cells. Spheroid growth in 3D with CPDD+PEM or CPDD+PEM+2-DG lowered spheroid growth in all cell types., Conclusion: 2-DG synergizes with CPDD+PEM in lowering MPM cell proliferation in 2D to <20%. In 3D MPM spheroid growth 2-DG synergism with CPDD+PEM treatment is not maintained., (Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2019
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26. Mediterranean Diet and 10-year (2002-2012) Incidence of Diabetes and Cardiovascular Disease in Participants with Prediabetes: The ATTICA study.
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Filippatos TD, Panagiotakos DB, Georgousopoulou EN, Pitaraki E, Kouli GM, Chrysohoou C, Tousoulis D, Stefanadis C, and Pitsavos C
- Subjects
- Adult, Biomarkers blood, Blood Glucose analysis, Cardiovascular Diseases epidemiology, Cardiovascular Diseases ethnology, Cardiovascular Diseases etiology, Cohort Studies, Diabetes Mellitus, Type 2 epidemiology, Diabetes Mellitus, Type 2 ethnology, Diabetes Mellitus, Type 2 etiology, Female, Follow-Up Studies, Greece epidemiology, Humans, Incidence, Male, Middle Aged, Nutrition Surveys, Prediabetic State blood, Prediabetic State ethnology, Prediabetic State physiopathology, Prospective Studies, Risk Factors, Cardiovascular Diseases prevention & control, Diabetes Mellitus, Type 2 prevention & control, Diet, Mediterranean ethnology, Patient Compliance ethnology, Prediabetic State diet therapy, Urban Health ethnology
- Abstract
Background: Prediabetes has been related to an increased risk of developing diabetes and cardiovascular disease (CVD)., Aim: The aim of the present study was to examine the effect of the Mediterranean diet on diabetes and CVD risk in subjects with impaired fasting glucose (IFG, i.e. fasting plasma glucose 100-125 mg/dl)., Methods: During 2001-2002, 3042 men and women (>18y) were enrolled for the study. The participants showed no clinical evidence of CVD or any other chronic disease, and were living in the greater Athens (Greece) area. In 2011 and 2012, the 10-year follow-up examinations were performed, including a working sample of n = 1875 participants without diabetes at baseline. Adherence to the Mediterranean diet at baseline evaluation was assessed using the MedDietScore (range 0-55)., Results: The prediabetic subjects (n = 343) had a significantly higher incidence of diabetes (25% vs. 10%, p < 0.001) and CVD (17.8% vs. 12.3%, p = 0.007) compared with subjects with normal glucose values. A significant trend towards lower diabetes and CVD incidence was observed with medium and high adherence to the Mediterranean diet compared with low adherence (p < 0.001). High adherence to the Mediterranean diet (>35/55 score) was associated with lower 10-year incidence of diabetes and CVD. In multiple logistic regression models, participants with high levels of adherence to the Mediterranean diet were significantly less affected by diabetes and CVD than those with low adherence levels., Conclusion: High adherence to the Mediterranean diet is associated with a low risk of developing diabetes and CVD in prediabetic subjects.
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- 2016
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27. Education status determines 10-year (2002-2012) survival from cardiovascular disease in Athens metropolitan area: the ATTICA study, Greece.
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Panagiotakos D, Georgousopoulou E, Notara V, Pitaraki E, Kokkou E, Chrysohoou C, Skoumas Y, Metaxa V, Pitsavos C, and Stefanadis C
- Subjects
- Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Alcohol Drinking, Cardiovascular Diseases mortality, Diet, Female, Greece epidemiology, Health Behavior, Health Status Disparities, Humans, Incidence, Male, Middle Aged, Risk Factors, Sex Factors, Smoking epidemiology, Socioeconomic Factors, Survival Analysis, Young Adult, Cardiovascular Diseases epidemiology, Educational Status, Life Style
- Abstract
Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality worldwide, and educational level seems to be an important determinant of the disease occurrence. The aim of this work was to investigate the association between education status and 10-year incidence of CVD, controlling for various socio-demographic lifestyle and clinical factors. From May 2001 to December 2002, 1514 men and 1528 women (>18 years) without any clinical evidence of CVD or any other chronic disease, at baseline, living in greater Athens area, Greece, were enrolled. In 2011-2012, the 10-year follow-up was performed in 2583 participants (15% of the participants were lost to follow-up). Incidence of fatal or non-fatal CVD was defined according to WHO-ICD-10 criteria. Education status was measured in years of schooling. The 10-year incidence of CVD was 15.7% [95% confidence intervals (CI) 14.1%-17.4%], 19.7% in men and 11.7% in women (Pgender < 0.001). Age-and gender-adjusted analyses revealed that those with low education (<9 years of schooling) were 1.52 times more likely (95% CI 1.03-2.23%) to have CVD compared with those with high education (>12 years of schooling). People in the low education group had higher prevalence of hypertension, diabetes and dyslipidaemias, were more likely to be smokers and sedentary, had less healthy dietary habits, as compared with those in the high education group. When controlling for participants' medical history, smoking, dietary and lifestyle habits, low education was no longer significantly associated with CVD, illustrating the mediating effect of clinical and behavioural factors in the link between education and disease. It was of interest that low education status interacted with alcohol drinking, enhancing the adverse effect of low education on CVD risk (relative risk 1.44, 95% CI 0.94%-2.20%), after various adjustments made. In this study, it was concluded that low educational level was associated with increased CVD risk. This was mainly explained by the intermediate association of low education with unhealthy choices that consequently worsen clinical status., (© 2015 John Wiley & Sons Ltd.)
- Published
- 2016
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28. Metabolic syndrome, adherence to the Mediterranean diet and 10-year cardiovascular disease incidence: The ATTICA study.
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Kastorini CM, Panagiotakos DB, Chrysohoou C, Georgousopoulou E, Pitaraki E, Puddu PE, Tousoulis D, Stefanadis C, and Pitsavos C
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Biomarkers blood, Blood Glucose metabolism, Blood Pressure, Cardiovascular Diseases diagnosis, Cardiovascular Diseases epidemiology, Female, Follow-Up Studies, Greece epidemiology, Humans, Incidence, Inflammation Mediators blood, Lipids blood, Logistic Models, Male, Metabolic Syndrome diagnosis, Metabolic Syndrome epidemiology, Middle Aged, Multivariate Analysis, Odds Ratio, Patient Compliance, Principal Component Analysis, Protective Factors, Risk Assessment, Risk Factors, Time Factors, Weight Gain, Young Adult, Cardiovascular Diseases prevention & control, Diet, Mediterranean, Metabolic Syndrome diet therapy, Risk Reduction Behavior
- Abstract
Background and Aims: To better understand the metabolic syndrome (MS) spectrum through principal components analysis and further evaluate the role of the Mediterranean diet on MS presence., Methods: During 2001-2002, 1514 men and 1528 women (>18 y) without any clinical evidence of CVD or any other chronic disease, at baseline, living in greater Athens area, Greece, were enrolled. In 2011-2012, the 10-year follow-up was performed in 2583 participants (15% of the participants were lost to follow-up). Incidence of fatal or non-fatal CVD was defined according to WHO-ICD-10 criteria. MS was defined by the National Cholesterol Education Program Adult Treatment panel III (revised NCEP ATP III) definition. Adherence to the Mediterranean diet was assessed using the MedDietScore (range 0-55)., Results: Five principal components were derived, explaining 73.8% of the total variation, characterized by the: a) body weight and lipid profile, b) blood pressure, c) lipid profile, d) glucose profile, e) inflammatory factors. All components were associated with higher likelihood of CVD incidence. After adjusting for various potential confounding factors, adherence to the Mediterranean dietary pattern for each 10% increase in the MedDietScore, was associated with 15% lower odds of CVD incidence (95%CI: 0.71-1.06). For the participants with low adherence to the Mediterranean diet all five components were significantly associated with increased likelihood of CVD incidence. However, for the ones following closely the Mediterranean pattern positive, yet not significant associations were observed., Conclusion: Results of the present work propose a wider MS definition, while highlighting the beneficial role of the Mediterranean dietary pattern., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2016
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29. 10-year incidence of diabetes and associated risk factors in Greece: the ATTICA study (2002-2012).
- Author
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Koloverou E, Panagiotakos DB, Pitsavos C, Chrysohoou C, Georgousopoulou EN, Pitaraki E, Metaxa V, and Stefanadis C
- Subjects
- Adolescent, Adult, Age Factors, Aged, Aged, 80 and over, Blood Glucose analysis, Body Mass Index, Diabetes Mellitus, Type 2 genetics, Diabetes Mellitus, Type 2 prevention & control, Energy Intake, Exercise, Fasting, Female, Follow-Up Studies, Greece epidemiology, Humans, Life Style, Logistic Models, Male, Middle Aged, Obesity epidemiology, Odds Ratio, Prospective Studies, Risk Factors, Waist-Height Ratio, Diabetes Mellitus, Type 2 epidemiology
- Abstract
Background: The incidence of diabetes in the general population is increasing world-wide. The increase is attributed to the consumption of saturated fatty acids, obesity, lack of physical activity, genetic predisposition, and other factors, but knowledge about the reasons, biological mechanisms, and late complications is insufficient. It is therefore important to clarify the reasons more exactly through long-term clinical trials to stop the rise of diabetes and its complications., Aim: To evaluate the 10-year incidence of type 2 diabetes in apparently healthy Greek adults., Methods: In 2001-2002, a random sample of 1514 men (18-87 years old) and 1528 women (18-89 years old) was selected to participate in the ATTICA study. During 2011-2012, the 10-year follow-up was performed. Patients diagnosed with diabetes at baseline (n = 210) and those lost at the 10-year follow-up (n = 1347) were excluded, yielding a final sample of 1485 participants., Results: During the period of investigation, diabetes was diagnosed in 191 cases corresponding to a 12.9% incidence (95%CI: 10.4-15.4), with 13.4% (95%CI: 10.8-16) in men and 12.4% (95%CI: 10.1-14.7) in women. A relative increase was observed in the second half of the 10-year follow-up when age became significant. Multiple logistic regression analysis revealed that age (OR = 1.14, 95%CI: 1.09-1.19), abnormal waist-to-height ratio (OR = 3.27, 95%CI: 1.07-10.0), fasting blood glucose (OR per 1 mg/dl = 0.05, 95%CI: 1.02-1.08), energy intake (OR per 500 kcal = 1.02, 95%CI: 1.01-1.35), and family history of diabetes (OR = 2.8, 95%CI: 1.30-6.03) were the most significant baseline predictors for diabetes, after adjusting for potential confounders. Waist-to-height ratio showed the best explanatory power of all anthropometric variables. Physical activity exerts an effect on risk factors. Being active was found to eliminate the aggravating effect of diabetes family history and fasting blood glucose., Conclusions: The findings confirm the escalating increase of type 2 diabetes incidence in Greece, which is in line with global trends. A lifestyle change in individuals at risk of developing diabetes towards healthier eating and increased physical activity would be an effective and inexpensive means of reducing diabetes.
- Published
- 2014
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