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Present study attempts to study the impact of Serlui-B hydroelectric dam on water quality ('water-energy nexus') of a Himalayan river and to devise possible phytotechnology for heavy metals remediation. Physico-chemical characteristics of water were analyzed using 'American Public Health Association (APHA)' methods while Atomic Absorption Spectrophotometer (AAS) was used for estimation of heavy metals. To this end, concentrations of Fe and Ni were recorded above permissible limit which can impose public health risks. Therefore, phytoremediation experiments were performed by using Pistia stratiotes L. (water lettuce) and effects of Fe and Ni concentrations (1.0, 2.0, and 5.0 mg/L) on photosynthetic pigments were observed. Phytoremediation investigations using 24 aquaria (of 40.5 L) revealed that P. stratiotes was an effective hyperaccumulator of Fe and Ni with maximum removal efficiency of 96.2% and 92%, respectively. In relation to bioaccumulation indices, the bioconcentration factor (BCF) values decreased with increase in Fe and Ni concentrations, whereas the translocation factor (TF) was noted higher at low concentrations and vice versa. Furthermore, the total chlorophyll content and carotenoid levels in P. stratiotes demonstrated negative significant correlation with the rise in Fe/Ni concentrations that may be considered as an adaptive biochemical strategy to mitigate metals induced oxidative stress. The present study can further be extrapolated to field phytoremediation for ameliorating both physico-chemical and metallic contaminants. Last, the role of microbial biodiversity associated with P. stratiotes should be elucidated in future to help achieve SDG 6 and environmental sustainability. [ABSTRACT FROM AUTHOR]

The textile industry flourished swiftly globally due to rapid industrialization. Consequently, hazardous and toxic dyes discharged into aquatic ecosystems adversely impact the stability of the ecosystem, plants and humans. This study focused on cost-efficient, eco-friendly and sustainable techniques to remediate textile effluents. Phytoremediation using Pistia stratiotes and biologically synthesized copper nanoparticles were used to remove mixed dyes. The size of the CuO NPs ranged from 16 nm – to 32 nm. Optimum degradation up to 68.2% was attained at 500 mg/L utilizing Pistia stratiotes. A kinetic study revealed that 50 mg of copper oxide nanoparticles could effectively degrade different concentrations of mixed dyes. Wastewater analysis, such as TDS, Nitrate, Chloride, DO and BOD, was carried out for five days. Histopathological examination revealed that mixed dye affected the leaves and roots of Pistia stratiotes. Phytotoxicity analysis was performed using PTC technique employing Vigna radiata which exhibited tolerance against highest and lowest concentration of dye aiding seed germination at 10 and 100 mg/L. When both phytoremediation and nanoparticles were implemented, mixed dye removal efficacy increased from 28.2% to 45.7%. Hence, it was inferred from the present study that an integrated approach enhanced the removal of mixed dyes from the textile effluents. [ABSTRACT FROM AUTHOR]

The phytoremediation potential of floating aquatic plants to accumulate and remove two common PFAS from contaminated water was investigated. Free-floating hydrophytes Eichhornia crassipes and Pistia stratiotes were grown in water spiked with 0.5, 1, or 2 ppm perfluorooctanoic acid (PFOA) or perfluorooctanesulfonic acid (PFOS) for seven days. Both species were able to accumulate PFOA and PFOS in this time frame, with translocation factors (TF) ranging from 0.13 to 0.57 for P. stratiotes and 0.18 to 0.45 for E. stratiotes, respectively. E. crassipes accumulated a greater amount of PFOA and PFOS than P. stratiotes, with 178.9 ug PFOA and 308.5 ug PFOS removed by E. crassipes and 98.9 ug PFOA and 137.8 ug PFOS removed by P. stratiotes at the highest concentrations. Root tissue contained a higher concentration of PFOA and PFOS than shoot tissue in both species, and the concentration of PFOS was generally significantly higher than PFOA in both E. crassipes and P. stratiotes, with concentrations of 15.39 and 27.32 ppb PFOA and 17.41 and 80.62 ppb PFOS in shoots and roots of P. stratiotes and 12.59 and 37.37 ppb PFOA and 39.92 and 83.40 ppb PFOS in shoots and roots of E. crassipes, respectively. Both species may be candidates for further phytoremediation studies in aquatic ecosystems. This study investigates the feasibility of using wetland plants for the phytoremediation of PFAS. Prior published studies examine various plant interactions with PFAS but do not evaluate remediation potential of P. stratiotes. [ABSTRACT FROM AUTHOR]

Abstract The objective of the study was to evaluate the performance of Pistia stratiotes for treatment of domestic wastewater in a free surface water flow constructed wetland. The objective of the study was to evaluate contaminants removal efficiency of the constructed wetland vegetated with P. stratiotes in treatment of domestic wastewater against Hydraulic retention time (HRT) of 10, 20 and 30 days was investigated. This asks for newer and efficient low-cost nature-based water treatment system which along with cost takes into consideration the sustainability of the ecosystem. Five constructed wetland setups improved the wastewater quality and purify it significantly by reducing the TDS by 83%, TSS by 82%, BOD by 82%, COD by 81%, Chloride by 80%, Sulfate by 77%, NH3 by 84% and Total Oil and Grease by 74%. There was an increase in pH of about 11.9%. Color and odor of wastewater was also improved significantly and effectively. It was observed that 30 days’ HRT was optimum for the treatment of domestic wastewater. The final effluent was found to be suitable as per national environmental quality standards and recycled for watering plants and crop irrigation but not for drinking purposes. The treatment in constructed wetland system was found to be economical, as the cost of construction only was involved and operational and maintenance cost very minimal. Even this research was conducted on the sole purpose of commuting the efficiency of pollutant removal in short span time.

Diabetes mellitus (DM) is a metabolic disorder that has a significant impact on the quality of life and health. This condition is indicated by high blood glucose or hyperglycemia. Alpha-glucosidase is an important enzyme during carbohydrate digestion to obtain monosaccharides such as glucose. This study aimed to investigate the alpha-glucoside inhibitory activity of polysaccharides from water lettuce (Pistia stratiotes) leaf with different extraction methods. The leaf was extracted by hot water extraction (HWE) and ultrasoundassisted extraction (UAE). The total sugar, crude fiber, and alpha-glucosidase inhibitory activity were analysis by in vitro, then an independent-sample t-test was used to analyze the statistically different. The UAE method showed a high yield of crude extract than the HWE method. However, the total sugar of HWE is higher than UAE, whereas no different of crude fiber level. The extract from the HWE method more effectively inhibits the alpha-glucosidase activity when compared to UAE. Therefore, hot water extraction is the suitable method to extract polysaccharides from a water lettuce (Pistia stratiotes) leaf. This condition also indicated polysaccharides extract potential to develop as an antidiabetes agent. [ABSTRACT FROM AUTHOR]

A monophage herbivorous insect called S. pectinicornis can be utilized to biologically control the weed Pistia stratiotes. Insect mass reproduction followed by release into the target weed area is one technique that can be used. The quality of the feed must be taken into consideration during maintenance in order to increase the fitness of S. pectinicornis insects and enable them to function at their best. The fertilization procedure can improve the nutritional value of P. stratiotes as a feed source. The experiment involved applying NPK fertilizer to P. stratiotes at 6 different levels: 0 g (control); 5 g; 10 g; 15 g; 20 g; and 25 g. The findings demonstrated that when NPK fertilizer was applied to Pistia stratiotes, S. pectiniconis larvae, pupae, and imago had a better chance of surviving than controls. S. pectinicornis showed improved insect growth and development since its lifespan was shorter than controls at every developmental stage. The ideal NPK fertilizer dose for maintaining Pistia stratiotes as feed during S. pectinicornis propagation is 15g/20 L of water.

Tailing land reclamation aims to improve the environment so that the tailing areas become productive for crop cultivation. The research was carried out in the tailings area of Mile 21.5 Timika from March to September 2022. The research aimed to study the dosage levels of Pistia stratiotes composts as an ameliorant to support the growth and yield of chili cultivated in the fresh tailings area. This study was laid out in a randomized block design with the Pistia stratiotes compost as a treatment, which consisted of PS1 = 1 kg/plant, PS2 = 2 kg/plant, PS3 = 3 kg/plant, and PS4 = 4 kg/plant in 6 replications. Based on the results, there was a significant effect of Pistia stratiotes compost on the growth and yields of chili. PS4 treatment produced the highest growth (plant height and leaf number) and highest yields (fruit number/plant, fruit weight/plant, fruit weight/plot, fruit weight/ha), while PS1 produced the lowest growth and yields. Chili produced 4.19 t/ha by PS4, indicating a high yield potential when tailings turned into a more stable growth media over time. There was a significant correlation between plant height and leaf number with the fruit weight. This shows that the vegetative growth component is closely related to increased chili yields. The high heritability value indicates the genetic factor influenced the growth and yields of chili more than the environmental factors. The metal contents in the chili fruit tissue are below the threshold level. The findings of this study emphasized the appropriate reclamation approaches using Pistia stratiotes compost as an ameliorant, which effectively improves the quality of tailing as growing media to maximize chili yields.

This study was conducted to investigate wastewater phytoremediation under different conditions. We examined the potential of Lemna minor and Pistia stratiotes in two proposed models in natural and artificial plant environment conditions and in previous studies (laboratory pilot-scale study). Before and after treatment, heavy metals, physical and chemical characteristics of wastewater and removal efficiency were analyzed. The results showed that in artificial conditions, Lemna minor reduced pollutants with efficiencies ranging from 90.3 to 98.06% for biological oxygen demand, chemical oxygen demand, total dissolved solids, salinity, conductivity, turbidity, total phosphorus, and total nitrogen, with an increase in saturated oxygen concentration of 82.9%. In natural conditions, the reduction efficiencies ranged from 55.9 to 89.9%, with an increase in saturated oxygen concentration of 67.2%. Conversely, in artificial conditions, Pistia stratiotes achieved removal efficiencies ranging from 93.2 to 98.9%, with an increase in saturated oxygen concentration of 83.6%. Efficiencies ranged from 70.1 to 91.9% in natural conditions. Similarly, the percentages of metals removal by Pistia stratiotes ranged from 71.4 to 97.8% for Fe, Ni, Pb, Cd, Zn, Co, and Cu in artificial conditions and from 37.9 to 79.8% in natural conditions. In addition, metal removal percentages by Lemna minor ranged from 89.6 to 96.4% in artificial conditions and from 27.9 to 60.1% in natural conditions. [ABSTRACT FROM AUTHOR]

Tailing land reclamation aims to improve the environment so that the tailing areas become productive for crop cultivation. The research was carried out in the tailings area of Mile 21.5 Timika from March to September 2022. The research aimed to study the dosage levels of Pistia stratiotes composts as an ameliorant to support the growth and yield of chili cultivated in the fresh tailings area. This study was laid out in a randomized block design with the Pistia stratiotes compost as a treatment, which consisted of PS1 = 1 kg/plant, PS2 = 2 kg/plant, PS3 = 3 kg/plant, and PS4 = 4 kg/plant in 6 replications. Based on the results, there was a significant effect of Pistia stratiotes compost on the growth and yields of chili. PS4 treatment produced the highest growth (plant height and leaf number) and highest yields (fruit number/plant, fruit weight/plant, fruit weight/plot, fruit weight/ha), while PS1 produced the lowest growth and yields. Chili produced 4.19 t/ha by PS4, indicating a high yield potential when tailings turned into a more stable growth media over time. There was a significant correlation between plant height and leaf number with the fruit weight. This shows that the vegetative growth component is closely related to increased chili yields. The high heritability value indicates the genetic factor influenced the growth and yields of chili more than the environmental factors. The metal contents in the chili fruit tissue are below the threshold level. The findings of this study emphasized the appropriate reclamation approaches using Pistia stratiotes compost as an ameliorant, which effectively improves the quality of tailing as growing media to maximize chili yields. [ABSTRACT FROM AUTHOR]

Pistia stratiotes L. is an invasive floating plant that alters native habitats in Florida by forming thick mats that shade out submerged vegetation and obstruct navigation. Multiple genotypes of this plant have been identified from locations across its native and adventive ranges including types from throughout the Americas, the Caribbean, Asia, and Australia. We investigated the performance of a known monophagous insect, Lepidelphax pistiae, on nine P. stratiotes haplotypes (from four clades) in no-choice experiments and found that while L. pistiae performance varied on different haplotypes, there was considerable overlap in fitness and impact among haplotypes. Lepidelphax pistiae did not distinguish between purported 'native' and 'non-native' haplotypes and did not perform better or cause more damage to South American haplotypes specifically, which would have supported its utility as a biocontrol agent on the exotic haplotypes found in Florida. While L. pistiae is monophagous on P. stratiotes, it was not specific enough to differentiate consistently among the tested haplotypes and thus, may not be suitable as a biological control agent because of the potential threat they pose to native haplotypes of P. stratiotes in Florida. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Even though oxidative and inflammatory bursts are a big part of renal reperfusion injury (RI/R), Pistia stratiotes (PS) has been used for a long time to stop these overreactions. People have said that it can drop both blood sugar and cholesterol. Hence, the goal of this study was to show how PS changed kidney reperfusion damage in both diabetic and normal rats. MATERIALS AND METHODS: In the study, 30 min of renal ischemia (RI) was followed by 1 h of recovery for each rat. Before the test, PS (100 mg/kg p. o.) was given to the animals for 7 days. Then, using the mixture from the separated kidney tissues, the antioxidant, inflammation, and histopathological effects were determined. RESULTS: When compared to RI/R, diabetic rats given PS had lower blood sugar, aspartate aminotransferase, blood urea nitrogen, and creatinine, myeloperoxidase, C-reactive protein, and tumor necrosis factor-alpha levels in their urine. CONCLUSION: PS potentially worked in hyperglycemic rats protecting them against RI/R. It is possible that PS's ability to protect the kidneys of the test rats is due to its ability to fight free radicals, lower blood sugar, and stop inflammation. [ABSTRACT FROM AUTHOR]

Eichhornia crassipes and Pistia stratiotes are weeds that often pollute water areas. These two contain various active compounds that act as antimicrobials, which have the potential as disinfection agents to prevent the transmission of pathogenic bacteria and fungi. An antimicrobial substance is said to be effective as a good disinfectant if it has a phenol coefficient value equal to or more than the 5% phenol comparison substance. The aim of this research was to test the effectiveness of a combination of Eichhornia crassipes and Pistia stratiotes extracts as a disinfection candidate through the phenol coefficient test. This in vitro experimental study was carried out using a dilution technique. The combination treatment of Eichhornia crassipes and Pistia stratiotes extracts and 5% phenol was tested against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and also Candida albicans yeast. Observations on the test media suspension tubes that were clear or did not show the growth of the test microbes for a period of 5, 10, and 15 minutes. The phenol coefficient value of the combination treatment of Eichhornia crassipes and Pistia stratiotes extracts was ≥1 with 5% phenol as comparison. Higher coefficient values were obtained for Staphylococcus aureus, equivalent values of 5% phenol were found for E.coli, P.aeruginosa, and C.albicans; whereas in S.typhi the value is

With the burgeoning population, the community activities, such as laundering, washing, or even bathing, have expanded along the irrigation canal. Consequently, some chemicals from those activities are released and pollute the water body. Besides, the discharge of wastewater into the irrigation system is also an issue of public health concern, because it creates an environment conducive to the reproduction of any pathogenic agent. Using candung as constructed wetlands (CW)-like to treat the irrigation water in rice paddies would be an interesting tool for removing pollutants from the irrigation water. Candung, as a locally used CW in Bali Island, has been applied in the rice field long time ago. The present study aimed to examine candung as a local attached CW or treating irrigation water and analyse the community awareness regarding the candung application. The result showed a positive effect of candung in maintaining the water quality parameters, such as TSS, BOD, COD, and nutrients with certain aquatic plants (Nelumbo nucifera, Nymphaea tetragona, and Pistia stratiotes). The knowledge, behaviour, and action of the local subak community were still limited, whereas they had positive perceptions and responses about candungthe potential of candung as an agrotourism attraction in the village as well

This study presents an eco-friendly method of synthesis plasmonic gold-titanium (TiO2@Au) nanoparticles, which exhibit enhanced photocatalytic degradation of ciprofloxacin under visible light. The treated effluent was then used to cultivate aquatic plants such as Pistia stratiotes, commonly referred to as water lettuce. In order to verify the success of the synthesis of the green plasmonic gold-titania nanoparticles, various spectroscopic techniques were utilized, including UV–Visible, Raman, Fourier Transform Infrared (FTIR), Powder X-ray Diffraction (XRD), Energy Dispersive X-ray (XPS), and microscopic Transmission Electron Microscopy (TEM) analysis. It is apparent from these images that these nanoparticles, with a diameter of about 10 ± 5 nm, are uniformly distributed on the surface of the TiO2 Anatase nanoparticles. Under visible light conditions, the nanocomposite removes 95 % of a high concentrated solution of ciprofloxacin, as demonstrated the UV–Vis spectrum. The phytotoxicity for one (01) week of the treated effluents demonstrated that the effluent is not harmful for the aquatic plant growth in comparison to the 50 ppm ciprofloxacin solution which exhibits abnormal plant growth. Based on literature reports and observed phenomena, the study also presents a plausible mechanism for the phenomenon.

Environmental pollution and degradation caused by the processing and refining of crude oil has been on the increase since its discovery in the Niger Delta region of Nigeria. Its negative impacts are unquantifiable though its refined products are of immense benefits to the nation and the world at large. An assessment of the performance of Pistia stratiotes as affected by the water soluble fraction of Universal Energy Akwa Ibom crude oil was carried out in Abraka, Delta State, Nigeria in 2023. The five levels of WSF (0, 10, 25, 50, 75 and 100%) were used. Pistia stratiotes thalli were obtained from below the Bridge along River Ethiope and exposed to the different treatments. The results showed a significant reduction (p≤0.05) in all the plant parameters measured (leaf area, plant number, fresh weights, dry weight biomass, relative growth rate and survival percentage) in plants grown in the WSF media when compared to their counterparts exposed to the control plots. Against the normal healthy plants observed in the control, signs of dieback, yellowness of leaves, suppress growth and deaths were observed in plants subjected to the water soluble fraction of the crude oil and the effects were WSF dose dependent. There was gradual reduction in the shoot biomass of the plant with increasing level of WSF. There is a gradual increase in the root biomass although there was a total root growth. The study has established that the performance of Pistia stratiotes was significantly affected by the presence of water soluble fraction of crude oil. The study has great implication on water biology and food security. Continuous environmental monitoring and remediation exercises should be conducted in oil producing communities by the government. [ABSTRACT FROM AUTHOR]

Obesity is a metabolic disorder disease and excessive fat accumulation. Synthetic antiobesity (antihyperlipidemic) drugs are considered to cause some side effects. Therefore, the investigation of novel antiobesity agents, with less adverse effects, is a major challenge in the future research, such as from plant extracts. Thus, this research aimed to investigate the antihyperlipidemic of purified polyphenol from water lettuce (Pistia stratiotes) extract. The research was conducted using crude and purified extracts of water lettuce. The purification process was performed by solid-phase extraction by using a HyperSep Retain PEP cartridge. The qualitative analysis of bioactive compounds (flavonoid, alkaloid, terpenoid, tannin) and quantitative analysis of lipase inhibition were conducted in the laboratory. The results showed that flavonoid, alkaloid, and tannin were increased after the purification process, whereas triterpenoid was reduced. The stretching of a polymeric hydroxyl group (O-H) and H-bonded stretching at 3382.36 cm-1 (crude extract) and 3399.25 cm-1 (purified extract) indicate present of phenol in the extracts. The inhibition of lipase enzyme also increased from 37.50% (crude extract) to 72.50% (purified extract). Therefore, purified polyphenol compound from water lettuce (Pistia stratiotes) extract is the potential agent as antihyperlipidemic agent. [ABSTRACT FROM AUTHOR]

With the burgeoning population, the community activities, such as laundering, washing, or even bathing, have expanded along the irrigation canal. Consequently, some chemicals from those activities are released and pollute the water body. Besides, the discharge of wastewater into the irrigation system is also an issue of public health concern, because it creates an environment conducive to the reproduction of any pathogenic agent. Using candung as constructed wetlands (CW)-like to treat the irrigation water in rice paddies would be an interesting tool for removing pollutants from the irrigation water. Candung, as a locally used CW in Bali Island, has been applied in the rice field long time ago. The present study aimed to examine candung as a local attached CW or treating irrigation water and analyse the community awareness regarding the candung application. The result showed a positive effect of candung in maintaining the water quality parameters, such as TSS, BOD, COD, and nutrients with certain aquatic plants (Nelumbo nucifera, Nymphaea tetragona, and Pistia stratiotes). The knowledge, behaviour, and action of the local subak community were still limited, whereas they had positive perceptions and responses about candungthe potential of candung as an agrotourism attraction in the village as well. [ABSTRACT FROM AUTHOR]

There is a growing need to reclaim wastewater for agricultural use due to freshwater limitation. Integrating macrophytes in vermifiltration improves the treatment efficiency. However, the effect of treated wastewater on seed germination and seedling development is not widely investigated. The study investigated the use of Pistia stratiotes in a macrophyte-assisted vermifiltration of domestic wastewater and assessed the effect on seed and seedling development of Zea mays, Triticum aestivum and Sorghum bicolor. Three irrigation treatments of water were applied: macrophyte-assisted vermifiltration (VP), no macrophyte vermifiltration (VM) and potable water (PW) as the control. Results showed that VP had a removal of 41–44% EC, 65–67% turbidity, 52–65% TDS, 67–70% TSS, 29–34% COD, 42–46% BOD, 67–70% N total, and 74–78% P available compared to VM. VP treatment reduced inhibition in the morphological, physiological and biochemical developments of seed and seedling growth. The use of macrophyte-vermifiltered wastewater significantly (p < 0.05) increased the percentage of germination and the radical length of all seed species increased as well. In terms of the seedling development, seedling mortalities were significantly (p < 0.05) reduced and more than 75% chlorophyll pigments estimations (Cɑ, Cɓ, Cɑ+ɓ and Cx+c) were identified in all seedling species when macrophyte-vermifiltered wastewater was used to irrigate. HIGHLIGHTS Vermifiltration of domestic wastewater integrated with macrophytes proved effective.; Pistia stratiote was suitable as a macrophyte in the water treatment process.; Treated wastewater indicated a potential water source for irrigation.; Water treatment reduced inhibition in seed growth and development.; Macrophyte-assisted vermifiltration of domestic wastewater revealed a sustainable domestic wastewater treatment technology.;

This study was performed to evaluate the impact of microplastics and heavy metals (Pb, Cd, Cr, Cu, Zn, Ni) on sediments, water, aquatic plants (Pistia stratiotes, Alternanthera philoxeroides, and Ipomoea carnea), and fish (Labeo rohita) samples collected from five different sites in the Bajwat wetlands in Sialkot, Pakistan. The concentrations of Pb, Cd, and Cr were above the permissible limits devised by WHO in all the ecosystem components (i.e. sediments, water, plants, and fish) at all sites. The maximum amount of microplastic particles (2317 microplastic particles per kg of sediments) was recorded at Site 1. The filaments were the most commonly found type of microplastics. Plants and fish samples also showed considerable concentration of metals. The multivariate statistical analysis revealed anthropogenic sources of elevated concentrations of metal elements which could cause adverse biological effects in the ecosystem. [ABSTRACT FROM AUTHOR]

Neohydronomus affinis Hustache is known as the waterlettuce weevil (Figure 1). It is a biological control agent of the floating plant, waterlettuce, Pistia stratiotes Linnaeus (Araceae) (O’Brien & Wibmer, 1989; Center et al., 2002). Waterlettuce is found in tropical and subtropical regions of the world. It infests slow-moving streams, waterways, lakes, and rivers, forming dense floating mats that are composed of several rosettes connected by stolons (runner stems). The presence of waterlettuce mats diminishes biological diversity and generates obstructions for boating, waterflow, and aquatic organisms (Cordo & Sosa, 2000; Dray & Center, 2002). Even though waterlettuce was documented by explorer William Bartram in Florida before 1765 (plants-archive.ifas.ufl/plant-directory), it is listed as a class II prohibited aquatic plant in the State of Florida. There are three species in the genus Neohydronmus, all of which originate from South and Central America. These weevils are subaquatic, and their exoskeletons are covered in white scales with no water-resistant features. The three species are all associated with waterlettuce and feed exclusively on it (Center et al., 2002; O’Brien & Wibmer, 1989). Among them, N. affinis was first introduced as a biological control agent into Florida in 1987 at Torry Island and Kreamer Island at the south end of Lake Okeechobee (Dray et al., 1990; 2001). Since then, the insect has successfully established itself in peninsular Florida and is also reported in Louisiana (Center et al., 2002).

As a source of xylan, lignocellulose waste such as wheat bran and aquatic weed Pistia stratiotes were employed, together with potassium nitrate as a nitrogen source, which were optimized using the Box- Behnken design to increase the production of xylooligosaccharides. The yield of xylooligosaccharides increased to 2.05- fold in the optimized medium than non-optimized medium. The produced xylooligosaccharides showed a peak at 890-900 cm-1 confirming the presence of β-glycosidic linkages when detected by Fourier Transform Infra-Red (FT IR). The prebiotic effect of produced xylooligosaccharides was studied on two strains named Lactococcus lactis Cl MN515342 and Lactococcus lactis Cp MN515343 isolated from curd samples. The growth of these Lactococcus strains was found to be stimulated. A log Colony Forming Unit/ml value of 7.813 and 8.146 for Cp and Cl was observed in a medium incorporated with XOS. The Short-Chain Fatty Acid (SCFA) production by Lactococcus lactis in a medium incorporated with xylooligosaccharides was detected by GCMS. The use of lignocellulosic waste for the production of xylooligosaccharides reduces the production cost. The xylooligosaccharides produced by Massilia timonae has shown promising result in stimulating the growth of Lactococcus lactis. Thus, XOS can be incorporated in curd or Yoghurt along with the Lactococcus lactis and used as synbiotic food. [ABSTRACT FROM AUTHOR]

The wastewater ensuing from public eateries is higher in its chemical and biological oxygen demand (COD and BOD) as also its oil, grease, and protein content than sewage. For this reason such wastewater is much harder to treat; its content of fats, proteinaceous material, and xenobiotics mounting major challenges. But in most of the developing world about 80% of such wastewater is discharged untreated and the remaining is mixed with sewage going to the treatment plants. This happens due to the prohibitively high cost of treatment that is entailed if these wastewaters are to be treated by conventional activated sludge processes (ASPs) or a combination of anaerobic digestion and ASPs. The practice of allowing eatery wastewater to join sewage en route sewage treatment plants increases the load on the latter, especially due to the high fat and protein content of the former. The present work describes attempts to use the recently developed and patented SHEFROL® technology in affecting treatment of wastewater coming from a typical eatery. After establishing feasibility at bench scale, the process was tested in a case study at pilot plant scale for treating 12,000 litres/day (LPD) of wastewater being generated by the eateries situated in the campus of Pondicherry University, India. The capacity of the pilot plant was then expanded to 30,000 LPD. Despite operating the units at a very low hydraulic retention time (HRT) of 2 ± 0.5 h, due to the limitations of land availability, which translates to a rate about three times faster than a typical ASP, over 50% removal of COD and BOD, and similarly substantial removal of other pollutants was consistently achieved. Given that the SHEFROL units can be set up at a negligible cost, the findings indicate that SHEFROL technology can be used to significantly yet inexpensively pre-treat eatery wastewaters before either sending them for further treatment to conventional sewage treatment plants, higher-end SHEFROL units, or discharging them directly if neither of the other two options is available. [ABSTRACT FROM AUTHOR]

Environmental pollution due to industrial activities has been reported since 1760, dating back to the first industrial revolution. One industrial activity that has led to major environmental degradation is coal mining, which can pollute surface and underground water due to acid mine drainage (AMD). Phytoremediation is low-cost, applicable, environmental, and does not generate other waste materials. In this research, we analyze the utilization of Eichhornia crassipes and Pistia stratiotes for AMD treatment. The results indicated that the AMD initially contained Fe, Mn, Al, Ca, and Mg. E. crassipes successfully reduced these contents by up to 69%, while P. stratiotes removed up to 62%. A cost analysis for phytoremediation of AMD is designed in terms of two schemes, with 4298 USD for the first scheme and no cost in the second scheme. The post-harvest potential, future research directions, and bibliometric analysis are also discussed. Overall, the results of this study indicate that P. stratiotes and E. crassipes are plants with great potential for AMD phytoremediation. [ABSTRACT FROM AUTHOR]

The highest waste generated in Malaysia is composed of municipal solid waste, which is mainly managed by landfilling. Heavy metals in leachate generated from landfill could have caused hazardous effects to human and environment. EAPR has been increasingly applied to treat soil and wastewater. This technique serves as a potential tool for remediation of real leachate. Metals (Mn, Cd, Fe, Ni, Pb, Zn) uptake by Pistia stratiotes were evaluated via flame atomic adsorption spectrophotometer. Pb and Fe could be the elements that were more efficiently removed by P. stratiotes in the EAPR system. The removal efficiency was 59.86 ± 9.98 and 56.56 ± 18.08% for Pb and Fe, respectively. EAPR significantly reduced the BOD (9.37 ± 2.36 mg/L), color (120.00 ± 5.77 PtCo), and turbidity (25.50 ± 11.96 NTU) of synthetic leachate. An obvious accumulation of heavy metals was observed at roots based on BCF and TF values. BCF values of Pb (18,999.06 ± 8,321.76) and Fe (16,090.81 ± 5,844.36) in the EAPR system were more than 103, which indicates that P. stratiotes is a hyperaccumulator. Further study on the upregulated genes is needed to comprehend the molecular basis of heavy metal stress tolerance. EAPR greatly reduced heavy metals, BOD, color, and turbidity of synthetic leachate. The use of water lettuce in EAPR could be another plant alternative for potential remediation of real leachate which is extremely difficult to be treated. [ABSTRACT FROM AUTHOR]

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As a signaling molecule, Nitric oxide (NO) has been widely used in abiotic stress mitigation studies. Pistia stratiotes showed a good synergistic removal effect on heavy metals, nitrogen and phosphorus, but the high concentration of copper(Cu) in swine wastewater inhibited the comprehensive removal ability of Pistia stratiotes. At present, it is not clear how the addition of NO regulates the stress resistance mechanism of Pistia stratiotes to copper in swine wastewater, and the microbial response mechanism accompanying this process is not yet clear. Therefore, in the concentration range of 0.31∼4 mg·L−1Cu2+ and NO concentration of 0,0.05 and 0.1 mg L−1, the removal effect of Pistia stratiotes on copper from swine wastewater was studied. The results showed as follows: The treatment of non-available copper in groups M and H increased by 10.67% and 22.31%, respectively, compared with that in group L. The critical point of inhibiting effect of NO on growth rate was 2.03 mg·L−1Cu. By measuring three-dimensional fluorescence spectrum, combined with parallel factor analysis and principal component analysis, it was confirmed that exogenous addition of NO affected the humification degree of dissolved organic matter(DOM) and promoted the chelation of organic matter with copper. With the increase of Cu concentration, the Reyranella and Prosthecobacter with certain copper resistance gradually gained advantages. Redundancy analysis(RDA) showed that Emiticicia had a strong correlation with the removal rates of ammonia nitrogen, total phosphorus and copper in swine wastewater, while hgcI_clade had a strong correlation with the removal rates of total nitrogen. In conclusion, controlling the dosage of NO can effectively improve the tolerance and removal effect of Pistia stratiotes on copper in swine wastewater, which is of great significance for promoting the treatment and resource transformation of swine wastewater. [Display omitted] • The copper ionic concentration in water of NO treatment is lower. • Inappropriate concentration of NO affected the adsorption of Cu by water lettuce. • NO affected the degree of DOM humification to alleviate copper stress. [ABSTRACT FROM AUTHOR]

Pistia stratiotes can not only effectively remediate eutrophic water, but also displays strong absorption and bioaccumulation abilities for heavy metals. However, it has not been well-understood how the plant resists the combined stress of heavy metals. In these experiments, the morphophysiological traits, the ascorbate-glutathione (AsA-GSH) cycle, the glyoxalase system, and the contents of zinc (Zn) and cadmium (Cd) were investigated under Zn and Cd co-pollution. The AsA-GSH cycle and glyoxalase system could coordinately alleviate the oxidative and carbonyl stress, which was identified as an important tolerance mechanism. With Zn50Cd1, Zn50Cd10, Zn100Cd1, and Zn100Cd10 treatments for 18 days, 90.75–93.69% of Zn and 88.13–96.96% Cd accumulated in the roots. Treatments with Zn50Cd50, and Zn100Cd50 for 18 days resulted in a decrease of stress tolerance and chlorophyll content in leaves, an increase in plasma membrane permeability, a massive accumulation of methylglyoxal (MG), and visible toxic symptoms. Additionally, the bioaccumulation factor (BCF) for roots and shoots and the translocation factor (TF) were >1, and the content of Cd in shoots was no <100 mg·kg−1. This indicated P. stratiotes was a Cd hyperaccumulator and have great potential for the phytoremediation of heavy metal contaminated water. Novelty statementPistia stratiotes, a cadmium hyperaccumulator, has great application potential for the phytoremediation of zinc and cadmium co-polluted water. [ABSTRACT FROM AUTHOR]

Pistia stratiotes (Araceae), commonly referred to as water lettuce, is one of the most notorious weeds that cause severe damage to the economy and natural ecosystems of infested areas. In order to explore the mechanism of its rapid invasion, here, we assembled a high‐quality chromosome‐level genome for P. stratiotes based on the Illumina sequencing, PacBio sequencing, and Hi‐C scaffolding technology. The assembled genome is 311.87 Mb in size with a contig N50 of 1.08 Mb. The contigs were further anchored on 14 pseudochromosomes with a scaffold N50 of 21.21 Mb. A total of 20,356 protein‐coding genes were predicted, of which 79.35% were functionally annotated here. Evolutionary analysis showed that P. stratiotes and Colocasia esculenta were clustered together as sister lineages that diverged approximately 61 Ma. The synteny analyses indicated that two whole‐genome duplication (WGD) events occurred within a short period in P. stratiotes. Moreover, comparative genome analysis indicated that the expansion of gene families corresponding to disease resistance might contribute to rapid invasion in P. stratiotes. Also, we analysed the disease‐resistance gene family (NBS‐LRR) involved in plant defence. A genome‐wide search in P. stratiotes genome identified 85 NBS‐LRR genes in this study. In conclusion, this study provides some new insights into the evolution of the invasive aquatic plant P. stratiotes. Our reference genome will also provide valuable resources for future invasion genomic research programmes. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Understanding the origins and genetic relationships of invasive, non-native species is critical to informing conservation and management practices. Pistia stratiotes is one such species—a pantropical floating plant that is problematic in many regions of the world, including Florida, USA. Questions surrounding the origins of P. stratiotes populations in Florida and elsewhere prompted a molecular investigation using five chloroplast and one mitochondrial DNA sequences. A total of 154 samples were collected from 14 countries. The sequence data was analyzed using haplotype network analysis, maximum likelihood phylogenetics and species delimitation tools. These data show that P. stratiotes comprises a minimum of seven distinct haplotypic clades worldwide, three of which differ enough to likely represent different species. Florida, which was more heavily sampled than other regions of the world, contains four of the clades—one of which shows evidence of being pan-Caribbean with sufficient variation to suggest regional (including Florida) nativity. A second clade, present in the U.S. Gulf States and California, may be native within this range, however more sampling is needed to fully describe its distribution and nativity. Another clade, predominant in southern Florida and the St. Johns River, likely originated in South America. Results are discussed in the broader context of the effects of cryptic species on weed management, including biological control efforts. [ABSTRACT FROM AUTHOR]

Background: The study was undertaken to determine the phytochemical screening and the antibacterial activities of Pistia stratiotes (water lettuce) against some clinically isolated fish pathogens. Methods: The healthy and disease free plants samples were collected from Jebba Upper Basin of River Niger and tested for their antibacterial activities and phytochemical properties. Pistia stratiotes aqueous extract was extracted using sterile distilled water. The Agar diffused methods were used to determine the antimicrobial actions of the plant against fish pathogens of Pseudomonas fluorescens, Escherichia coli, Salmonella typhi, Aeromonas hydrophila, and Vibrio anguillarum. Results: The antibacterial investigation of aqueous extracts revealed the significant activity against these bacterial. Moreover, phytochemical examination of the crude extract showed the occurrence of saponins, alkaloids, tannins, anthraquinones, flavonoids, and catechins, which completely inhibited the growth of V. anguillarum and E. coli. However, Salmonella typhi, Pseudomonas fluorescens, and Aeromonas hydrophila exhibited moderate varying levels of resistance. Conclusion: The finding of this study showed that bioactive resistance from Pistia stratiotes can help disrupt the activities of various fish bacterial in aquaculture practices

Copyright of Tecno-Lógica is the property of Tecno-Logica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Indonesian Fisheries Processing Journal / Jurnal Pengolahan Hasil Perikanan Indonesia is the property of IPB University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Pistia stratiotes is a cadmium (Cd) hyperaccumulating plant with strong bioaccumulation and translocation capacity for Cd. A hydroponic experiment was used to evaluate the combined effect of Zinc (Zn) and Cd at different concentrations on leaf growth and metabolism of P. stratiotes. This study revealed the physiological defense and metabolic strategy of responses to Zn–Cd co-pollution. With the Zn 50 Cd 1 , Zn 50 Cd 10 , Zn 100 Cd 1, and Zn 100 Cd 10 treatments for 9 d, the relative crown diameter, relative leave number, and ramet number of the plant had no significant difference with the control. Under the compound treatments containing Zn 50 Cd 50 and Zn 100 Cd 50 , the activity of the glyoxalase system and amino acid metabolism in the leaves were inhibited. The leaf photosynthetic apparatus increased heat dissipation to reduce the damage to the photosystem II (PS II) reaction center caused by excess excitation energy under Zn–Cd stress. This safeguarded the balance between the absorption and utilization of light energy. Compared to the control, the Zn and Cd co-pollution for 9 d had no effect on the reduced glutathione (GSH) and oxidized glutathione (GSSG) contents. There was no effect on the dehydroascorbate reductase (DHAR) and glutathione reductase (GR) activities, but there was increased ascorbate peroxidase (APX) activity and oxidized ascorbic acid (DHA) content. These increased the antioxidant capacity of the ascorbate-glutathione (AsA-GSH) cycle. The treated plants also had increased levels of carnosol and substances related to lipid metabolism including 9, 10-Dihydroxystearate, Prostaglandin G2, Sphingosine, and 13-L-Hydroperoxylinoleic acid, maintaining the cell stability and resistance to the Zn–Cd stress. [Display omitted] • The redox reaction equilibrium of glutathione maintained the antioxidant stability. • Increased carnosol and lipid metabolism were conducive to zinc-cadmium tolerance. • Pistia stratiotes can be used for phytoremediation of zinc-cadmium polluted water. [ABSTRACT FROM AUTHOR]

This study aims at determining the optimal conditions for pollutants removal in wastewater using Eichhornia crassipes (E. crassipes) and Pistia stratiotes (P. stratiotes) as appropriate aquatic plants for a post-treatment by phytoremediation. From factors such as residence time, plant density and initial PO43− concentration, four responses, i.e. the removal efficiency of PO43−, NO3−, NH4+ and the chemical oxygen demand (COD) were followed, using complete factorial design. After validation of the regression models by the statistical analyses, optimal conditions were obtained by using the global desirability function. Global desirabilities of 0.96 and 0.97 were respectively obtained for E. crassipes and P. stratiotes, for a residence time of 30 days, a plant density of 60 feet/m2 and an initial PO43− concentration of 10 mg/L. Using E. crassipes, this corresponds to the elimination of 94.2% of PO43−; 93.3% of NO3−; 95.0% of NH4+ and 63.6% of COD. In the case of P. stratiotes, 93.9% of PO43−; 83.4% of NO3−, 99.5% of NH4+ and 84.4% of COD were removed. Finally, under the used conditions, E. crassipes are able to better eliminate phosphorus and nitrates, while P. stratiotes are very effective in removing NH4+ and COD. [ABSTRACT FROM AUTHOR]

The pollution of heavy metals in aquatic environments is a major concern for human beings. The present study demonstrates the phytoremediation potential of the aquatic macrophyte Pistia stratiotes for removal of Cr, Pb and Ni from simulated wastewater. Pistia stratiotes was grown in Faculty of Science & Natural Resources (FSNR) lake water and spiked with different concentrations of heavy metals at 1 mg/L, 2 mg/L and 3 mg/L of Cr, Pb and Ni, respectively. The experiment was conducted within a 14-day period in laboratory conditions. The study investigated the percentage of removal of heavy metals by P. stratiotes as well as determining the distribution of heavy metal patterns in plant tissues, the bioconcentration factor (BCF), translocation factor (TF) and relative treatment efficiency index (RTEI). The results showed that P. stratiotes is efficient in removing single Pb at 1 mg/L and 3 mg/L and single Cr at 1 mg/L, with a removal efficiency of 99.31%, 79.86% and 76.25%, respectively. It was found that P. stratiotes managed to concentrate Pb in its roots up to 15,000 mg/kg in plant tissue. Data on bioconcentration factor (BCF) showed that P. stratiotes managed to reach BCF values over 6,000 each for single chromium at 2 mg/L and lead at both 2 mg/L and 3 mg/L. It was found that the plant can consistently translocate nickel from the roots to the shoots, while chromium and lead tend to concentrate in the root tissues. The results revealed that P. stratiotes uses rhizofiltration as its phytoremediation uptake mechanism. This study helps significantly to increase knowledge regarding the potential of P. stratiotes in the phytoremediation of heavy metal-polluted wastewater.

The foundation of most aquatic weed management programs in Florida is synthetic herbicides because many of these U.S. Environmental Protection Agency (USEPA)-registered products are effective, selective, and inexpensive compared with other strategies such as mechanical harvesting. However, stakeholders have expressed concern regarding their use and managers are interested in exploring alternative methods for aquatic weed control. To that end, we evaluated the efficacy, selectivity, and costs of the “natural” products acetic acid and d-limonene (alone and in combination with each other and citric acid) on the invasive floating plants waterhyacinth (Eichhornia crassipes) and waterlettuce (Pistia stratiotes), and the native emergent plants broadleaf sagittaria (Sagittaria latifolia) and pickerelweed (Pontederia cordata). These products, plus an industry-standard synthetic herbicide (diquat dibromide), were applied once as foliar treatments to healthy plants, which were grown out for 8 weeks after treatment to allow development of phytotoxicity symptoms. A 0.22% concentration of diquat dibromide eliminated all vegetation, but neither “natural” product alone provided acceptable (>80%) control of floating weeds, even when applied at the maximum concentrations under evaluation (20% acetic acid, 30% d-limonene). Citric acid (5% or 10%) had no effect on the activity of acetic acid or d-limonene, but some combinations of acetic acid and d-limonene controlled floating weeds effectively without causing unacceptable damage to native plants. However, these treatments are much more expensive than the synthetic standard and managers would realize a 22- to 26-fold increase in product cost alone without factoring in other expenses such as additional labor and application time. Combinations of acetic acid and d-limonene may have utility in some areas where the use of synthetic herbicides is discouraged, but broad-scale deployment of this strategy would likely be prohibitively expensive.

The freshwater plant water lettuce (Pistia stratiotes L.) grows in warm climatic zones and is used for phytoremediation and biomass production. P. stratiotes belongs to the Araceae, an ecologically and structurally diverse early monocot family, but the phylogenetic relationships among Araceae members are poorly understood. Ribosomal DNAs (rDNAs), including the 35S and 5S rDNA, encode the RNA components of ribosomes and are widely used in phylogenetic and evolutionary studies of various plant taxa. Here, we comprehensively characterized the chromosomal locations and molecular organization of 35S and 5S rDNA genes in water lettuce using karyological and molecular methods. Fluorescence in situ hybridization revealed a single location for the 35S and 5S rDNA loci, each on a different pair of the species' 28 chromosomes. Molecular cloning and nucleotide sequencing of 35S rDNA of P. stratiotes , the first representative Araceae sensu stricto in which such a study was performed, displayed typical structural characteristics. The full-length repeat showed high sequence conservation of the regions producing the 18S, 5.8S, and 25S rRNAs and divergence of the internal transcribed spacers ITS1 and ITS2 as well as the large intergenic spacer (IGS). Alignments of the deduced sequence of 18S rDNA with the sequences available for other Araceae and representatives of other clades were used for phylogenetic analysis. Examination of 11 IGS sequences revealed significant intra-genomic length variability due to variation in subrepeat number, with four types of units detected within the 35S rDNA locus of the P. stratiotes genome (estimated size 407 Mb/1C). Similarly, the 5S rDNA locus harbors gene units comprising a conserved 119-bp sequence encoding 5S rRNA and two types of non-transcribed spacer (NTS) sequences. Type I was classified into four subtypes, which apparently originated via progressive loss of subrepeats within the duplicated NTS region containing the 3' part of the 5S rRNA gene. The minor Type II NTS is shorter than Type I and differs in nucleotide composition. Some DNA clones containing two or three consecutive 5S rDNA repeats harbored 5S rDNA genes with different types of NTSs, confirming the mosaic composition of the 5S rDNA locus. [ABSTRACT FROM AUTHOR]

ABSTRACT Amazon wetlands are among the most vulnerable ecosystem to be impacted by climate change, which may increase the frequency of extreme droughts and floods. We used Eichhornia crassipes and Pistia stratiotes, two abundant aquatic plants in the Amazon floodplains, to evaluate the effects of combined temperature and [CO2] increase on growth, physiology and ecological interactions. Individual and paired plants were deposited for three weeks in a microcosm under four IPCC scenarios: control (current temperature/CO2), mild (control + 1.5 ºC, 200 ppm CO2), intermediate (control + 2.5 ºC, 450 ppm CO2) and extreme (control + 3.5 ºC, 850 ppm CO2). P. stratiotes died after three weeks in the intermediate and extreme treatments; E. crassipes experienced no mortality or change in any of the measured variables during the same period. P. stratiotes reduced root length in the mild treatment and reduced total dry biomass in intermediate and extreme treatments, revealing less tolerance to climate change. Ecological interactions between the two species changed with increasing [CO2] and temperature neutral interaction changed to facilitation for E. crassipes, while competitive interaction changed to neutral for P. stratiotes. Global climate change may alter the composition, biomass and ecological interactions of Amazonian aquatic plant species.

Thorium (Th) waste is an alpha-emitting radioactive waste that is harmful to the environment and living things so it is necessary to treat the waste effectively with phytoremediation. Phytoremediation is a plant ability to reduce pollutants presence in the environment. This research aim is examining apu wood plant ability to reduce Th contents from Th-contaminated waste. The UV-VIS spectrophotometer analysed Th level in apu wood plant and liquid waste while the Ludlum Model 1000 Scaler measured their radioactivity. The parameters for phytoremediation occurrence include the decreasing physical condition of the plants, the pH of the waste that is close to neutral, and the temperature that fluctuates with the ambient temperature. The results showed that the plant biomass of apu wood decreased after phytoremediation to 96.2% with a remediation efficiency of 97.4% from the initial concentration of Th liquid radioactive waste of 10 ppm. This is due to thorium absorption resulting in thorium accumulation in apu wood plants of 4,069.4 mg/kg. Apu wood was also able to reduce Th liquid waste radioactivity to 0.631 Bq/L from the initial radioactivity of 2.819 Bq/L. The radioactivity level is below the Klierens Level and it is safe to release the waste into the environment.