31 results on '"Pista, Ângela"'
Search Results
2. Phenotypic and Genotypic Characterization of Escherichia coli and Salmonella spp. Isolates from Pigs at Slaughterhouse and from Commercial Pork Meat in Portugal.
- Author
-
Gonçalves, Carlota, Silveira, Leonor, Rodrigues, João, Furtado, Rosália, Ramos, Sónia, Nunes, Alexandra, and Pista, Ângela
- Abstract
Background: Foodborne diseases are a serious public health concern, and food-producing animals are a major source of contamination. Methods: The present study analysed Escherichia coli and Salmonella spp. isolated from faecal samples of 100 fattening pigs and from 52 samples of pork meat. Results: The results showed that the majority of the analysed meat samples were considered satisfactory in terms of microbiological quality (92.3% for E. coli and 94.2% for Salmonella spp.). Salmonella spp. was identified in 5.8% of the meat samples, whereas E. coli was detected in 89.5% of all samples (69.2% in meat and 100% in faecal samples). Furthermore, 1.9% of the faecal samples contained Shiga-toxin-producing E. coli and 3.9% contained enterotoxigenic E. coli. All sequenced isolates presented virulence genes for extraintestinal pathogenic E. coli. Moreover, 75.0% of E. coli isolates from meat and 71.8% from faeces samples showed antibiotic resistance, with 40.7% and 51.4%, respectively, being multidrug-resistant (MDR). The most prevalent resistances were to tetracycline, ampicillin, and sulfamethoxazole, and one E. coli isolate showed resistance to extended-spectrum β-lactamase. Conclusions: This study highlights the role of pigs as a potential source of human contamination and the importance of a One Health approach to ensure food safety and to promote public health. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Unveiling a Listeria monocytogenes Outbreak in a Rabbit Farm: Clinical Manifestation, Antimicrobial Resistance, Genomic Insights and Environmental Investigation
- Author
-
Rodrigues, Inês C., primary, Ribeiro-Almeida, Marisa, additional, Silveira, Leonor, additional, Prata, Joana C., additional, de Carvalho, André Pinto, additional, Roque, Carla, additional, Gomes, João Paulo, additional, Borges, Vítor, additional, Pista, Ângela, additional, and Martins da Costa, Paulo, additional
- Published
- 2024
- Full Text
- View/download PDF
4. Gulls in Porto Coastline as Reservoirs for Salmonella spp.: Findings from 2008 and 2023
- Author
-
Rodrigues, Inês C., primary, Cristal, Ana Paula, additional, Ribeiro-Almeida, Marisa, additional, Silveira, Leonor, additional, Prata, Joana C., additional, Simões, Roméo, additional, Vaz-Pires, Paulo, additional, Pista, Ângela, additional, and Martins da Costa, Paulo, additional
- Published
- 2023
- Full Text
- View/download PDF
5. First Report of Salmonella Serovar Typhimurium and Monophasic Typhimurium Clinical Isolates Harboring mcr-9 in Portugal
- Author
-
Silveira, Leonor, primary and Pista, Ângela, additional
- Published
- 2023
- Full Text
- View/download PDF
6. Gulls in Porto Coastline as Reservoirs for Salmonella spp.: Findings from 2008 and 2023.
- Author
-
Rodrigues, Inês C., Cristal, Ana Paula, Ribeiro-Almeida, Marisa, Silveira, Leonor, Prata, Joana C., Simões, Roméo, Vaz-Pires, Paulo, Pista, Ângela, and Martins da Costa, Paulo
- Subjects
GULLS ,WHOLE genome sequencing ,SALMONELLA ,DRUG resistance in microorganisms ,HUMAN settlements ,SALMONELLA enterica ,SALMONELLA typhimurium - Abstract
Gulls act as intermediaries in the exchange of microorganisms between the environment and human settlements, including Salmonella spp. This study assessed the antimicrobial resistance and molecular profiles of Salmonella spp. isolates obtained from fecal samples of gulls in the city of Porto, Portugal, in 2008 and 2023 and from water samples in 2023. Antimicrobial susceptibility profiling revealed an improvement in the prevalence (71% to 17%) and antimicrobial resistance between the two collection dates. Two isolate collections from both 2008 and 2023 underwent serotyping and whole-genome sequencing, revealing genotypic changes, including an increased frequency in the monophasic variant of S. Typhimurium. qacE was identified in 2008 and 2023 in both water and fecal samples, with most isolates exhibiting an MDR profile. The most frequently observed plasmid types were IncF in 2008 (23%), while IncQ1 predominated in 2023 (43%). Findings suggest that Salmonella spp. circulate between humans, animals, and the environment. However, the genetic heterogeneity among the isolates from the gulls' feces and the surface water may indicate a complex ecological and evolutionary dynamic shaped by changing conditions. The observed improvements are likely due to measures to reduce biological contamination and antimicrobial resistance. Nevertheless, additional strategies must be implemented to reduce the public health risk modeled by the dissemination of pathogens by gulls. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
7. Intersectoral collaboration in a One Health approach: Lessons learned from a country-level simulation exercise
- Author
-
Pista, Ângela, Martins, João Vieira, Clemente, Lurdes, Rodrigues, Nuno Santos, Vasconcelos, Paula, Nabais, Pedro, Carvalho, Renata, Manageiro, Vera, Caria, Ana, Furtado, Cristina, Botelho, Ana, Oleastro, Mónica, and Gonçalves, Sandra Cavaco
- Published
- 2023
- Full Text
- View/download PDF
8. Risk factors for human papillomavirus infection among women in Portugal: The CLEOPATRE Portugal Study
- Author
-
Pista, Angela, de Oliveira, Carlos F., Cunha, Maria J., Paixao, Maria T., and Real, Odete
- Published
- 2012
- Full Text
- View/download PDF
9. Characterization of Multidrug-Resistant Isolates of Salmonella enterica Serovars Heidelberg and Minnesota from Fresh Poultry Meat Imported to Portugal
- Author
-
Silveira, Leonor, primary, Nunes, Alexandra, additional, Pista, Ângela, additional, Isidro, Joana, additional, Belo Correia, Cristina, additional, Saraiva, Margarida, additional, Batista, Rita, additional, Castanheira, Isabel, additional, Machado, Jorge, additional, and Gomes, João Paulo, additional
- Published
- 2021
- Full Text
- View/download PDF
10. Use of the NucliSENS EasyQ HPV assay in the management of cervical intraepithelial neoplasia
- Author
-
Oliveira, Ana, Verdasca, Nuno, and Pista, Ângela
- Published
- 2013
- Full Text
- View/download PDF
11. Multidrug-Resistant Salmonella enterica Serovar Rissen Clusters Detected in Azores Archipelago, Portugal
- Author
-
Silveira, Leonor, primary, Pinto, Miguel, additional, Isidro, Joana, additional, Pista, Ângela, additional, Themudo, Patrícia, additional, Vieira, Luís, additional, Machado, Jorge, additional, and Gomes, João Paulo, additional
- Published
- 2019
- Full Text
- View/download PDF
12. Phenotypic characterization of Salmonella enterica isolates received in INSA between 2014 and 2017
- Author
-
Leonor, Silveira, Pista, Ângela, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Portugal ,Infeções por Salmonella enterica ,Doenças Infeciosas ,Salmonella enterica ,Saúde Pública - Abstract
As infeções por Salmonella enterica são uma das causas mais frequentes de gastroenterite aguda em todo o mundo. Entre 2014 e 2017 foram recebidas no Laboratório Nacional de Referência de Infeções Gastrintestinais do Departamento de Doenças Infeciosas do Instituto Nacional de Saúde Doutor Ricardo Jorge (INSA) 1124 estirpes de Salmonella isoladas de doentes portugueses provenientes de várias zonas do país. Foram identificados 68 serotipos diferentes, sendo os mais frequentes S. 4,5:i:- (n=372; 33,1%), S. Enteritidis (n=335; 29,8%), S. Typhimurium (n=208; 18,5%), S. Rissen (n=28; 2,5%), S. Typhi (n=25; 2,2%) e S. Stanley (n=12; 1,1%). Os serotipos de Salmonella identificados no período entre 2014 e 2017 acompanham a tendência europeia, nomeadamente no que se refere à diminuição de S. 4,5:i:- e aumento de S. Enteritidis. A serotipagem e a utilização de metodologias moleculares são fundamentais para a monitorização das salmoneloses humanas, particularmente na deteção de surtos e identificação de estirpes resistentes. Neste contexto, é fundamental manter e promover a colaboração entre os diversos serviços de saúde nacionais e internacionais, de forma a garantir a vigilância e controlo das infeções gastrintestinais em geral e por Salmonella spp em particular. Salmonella enterica infections are one of the most frequent causes of gastrointestinal disease around the world. Between 2014 and 2017, 1124 Salmonella strains isolated from Por tuguese patients, from several areas of the countr y, were sent to the National Reference Laborator y for Gastrointestinal Infections of the Institute of Health Dr Ricardo Jorge of Por tugal for serot yping. Six t y eight dif ferent serot ypes were identif ied, and S. 4,5:i:- (n=372; 33,1%), S. Enteritidis (n=335; 29,8%), S. Typhimurium (n=208; 18,5%), S. Rissen (n=28; 2,5%), S. Typhi (n=25; 2,2%) and S. Stanley (n=12; 1,1%) were the most frequently detected. Salmonella serot ypes identif ied in Por tugal between 2014 and 2017 are in line with the European trend, namely with regard to the decrease of S. 4,5: i: - and increase of S. Enteritidis. Serot yping and molecular t yping are fundamental for salmonellosis monitoring, par ticularly outbreak and resistance detection. In this contex t, it is ex tremely impor tant to maintain and promote the collaboration of several national and international health ser vices, in order to ensure gastrointestinal infections control and vigilance, par ticularly Salmonella spp. infections. info:eu-repo/semantics/publishedVersion
- Published
- 2018
13. Phenotypic characterization of isolates of Shigella spp. between 2015 and 2017
- Author
-
Silveira, Leonor, Pista, Ângela, and Machado, Jorge
- Subjects
Saúde Pública ,Infecções Gastrintestinais ,Shigella spp ,Shigelose - Abstract
Em Portugal, a shigelose é uma gastroenterite pouco frequente. Com este estudo pretendeu-se descrever os serotipos de Shigella spp. identificados no Laboratório Nacional de Referência de Infeções Gastrintestinais do Instituto Nacional de Saúde Doutor Ricardo Jorge (INSA) entre 2015 e 2017. Foram analisadas estirpes isoladas de 53 doentes, que foram enviadas a nível nacional ao INSA para serotipagem. A suscetibilidade aos antimicrobianos foi realizada segundo as recomendações do European Committee on Antimicrobial Susceptibility Testing (EUCAST). Os serotipos mais frequentemente encontrados foram Sh. sonnei (n=37; 69,8%), Sh. flexneri 2 (n=7; 13,2%), Sh. flexneri 3 (n=3; 5,7%). Foi observada uma elevada percentagem de resistência à tetraciclina (47/53; 88,7%). Em 2017, todas as estirpes apresentaram resistência à ampicilina e a percentagem de estirpes com resistência à ciprofloxacina aumentou consideravelmente, de 5,0% em 2015 para 62,5% em 2017. Cerca de 50% das estirpes apresentaram resistência à azitromicina durante o período em análise. Foram detetados 4 casos de Shigella spp. multirresistentes em homens que fazem sexo com homens (HSH). O aumento de resistências aos antibióticos observados nestes dois anos alerta para a importância de uma vigilância ativa das mesmas e impõe uma articulação efetiva entre os diversos serviços de saúde envolvidos. Shigella is a rare cause of gastrointestinal disease in Portugal. This study describes Shigella serotypes identified in National Reference Laboratory for Gastrointestinal Diseases of National Institute of Health Doutor Ricardo Jorge (INSA) from 2015 to 2017. We analysed the strains isolated from fifty three patients. Antimicrobial susceptibility testing wasperformed according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The most frequent serotypes were Sh. sonnei (n=37; 69.8%), Sh. flexneri 2 (n=7; 13.2%), Sh. flexneri 3 (n=3; 5.7%). We observed a high frequency of resistance to tetracycline (47/53; 88.7%). In 2017, all strains presented resistance to ampicillin and the percentage of strains resistant to ciprofloxacin increased from 5.0% in 2015 to 62.5% in 2017. About 50% of the strains were resistant to azithromycin. We detected four cases of multiresistant Shigella spp. in men who have sex with men (MSM). The increase of resistant strains observed in these two years alerts to the importance of an active vigilance and to the need of an effective articulation of the several health services involved. info:eu-repo/semantics/publishedVersion
- Published
- 2018
14. The use of molecular typing for characterization of a Salmonella Enteritidis cluster
- Author
-
Silveira, Leonor, Pista, Ângela, Borges, Vítor, Silva, Catarina, Vieira, Luís, Gomes, João Paulo, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Characterization by Molecular Typing ,Salmonella enterica ,Salmonella Enteritidis ,Gastrointestinal Infections - Abstract
Although the number of Salmonella Enteritidis has been declining in Europe in recent years due to successful control measures implemented in poultry industry, it remains the most commonly detected serovar of human non-typhoidal salmonellosis. Here, we reported the first Salmonella Enteritidis outbreak investigated by the Portuguese Reference Laboratory for Salmonella where traditional typing data was reinforced by Whole Genome Sequencing (WGS) data. In July 2016 the National Reference Laboratory for Gastrointestinal Diseases of the National Institute of Health Doutor Ricardo Jorge received 10 strains of Salmonella from a suspected human outbreak in Lisbon and Tagus Valley region. Serotyping was performed according to Kauffman-White-Le Minor scheme and antimicrobial susceptibility was tested according to EUCAST recommendations. Isolates were characterized by Multilocus Sequence Typing (MLST), Multiple-Locus Variable number tandem repeat Analysis (MLVA) and WGS. All strains were identified as S. Enteritidis ST11. Nine of them had the MLVA profile 3-11-5-4-1 and another strain had a rather different MLVA profile (2-10-9-2-2). This strain had also a different antibiotic resistance profile (being resistant only to sulphametoxazole while related MLVA 3-11-5-4-1 strains were resistant only to nalidixic acid), which reinforces that this isolate is very likely not associated with the outbreak. WGS analysis of four outbreak-related strains confirmed that these strains share the same serotype (Enteritidis) and MLST profile (ST11), allowed the in silico prediction of the same ribotype (rST 1425), and confirmed the genetic relatedness at whole-genome level. Unfortunately, no strains from any potential food sources were obtained. MLVA is traditionally used for Salmonella Enteritidis surveillance in Europe, since it is a rapid and reproducible high-resolution typing technique that allows detection of national and multinational outbreaks. However, WGS is progressively replacing the traditional geno- and phenotyping techniques for routine surveillance and outbreak investigation. This report is a good example not only that WGS provides an optimal discriminatory power for outbreak resolution, but also that allows performing several subtyping tests (ST determination, in silico serotyping and ribotyping) from a single assay. N/A
- Published
- 2018
15. Serotype distribution and antimicrobial resistance of clinical Salmonella enterica isolated in Portugal between 2014 and 2017
- Author
-
Silveira, Leonor, Pista, Ângela, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Salmonellosis ,Portugal ,Resistência aos Antimicrobianos ,Salmonella enterica ,Gastrointestinal Infections ,Food-borne Salmonellosis ,Antimicrobial Resistence - Abstract
Food-borne salmonellosis is an important public health concern worldwide. This study aimed to characterize the epidemiological patterns and resistance profile of clinical Salmonella enterica strains sent to the National Reference Laboratory for Gastrointestinal Infections of the National Institute of Health Doutor Ricardo Jorge (INSA), between January 2014 and November 2017. During the four-year period of study, 1082 clinical isolates were sent to INSA from several Portuguese hospitals and laboratories. Serotyping was performed according to Kauffman-White-Le Minor scheme and antimicrobial susceptibility was tested according to EUCAST recommendations. Multiple-Locus Variable number tandem repeat Analysis (MLVA) and Whole Genome Sequencing (WGS) was performed for outbreaks identification. Among the 1082 isolates received, 83.0% were isolate in faeces, 10.8% in blood, 2.1% in faeces and blood, and 4.1% in other biological products. Fifty-five serotypes were identified. The top five most frequent serotypes, Salmonella 4,5:i:- (33.6%), Salmonella Enteritidis (29.3%), Salmonella Typhimurium (18.4%), Salmonella Rissen (2.3%) and Salmonella Typhi (2.3%), accounted for 85.9% of fully serotyped isolates. All cases of S. Typhi were acquired in endemic zones. Antibiotic susceptibility was tested in 943 isolates (2015-2017). Rates of resistance to at least one antibiotic and to ≥ 4 antibiotics were observed in 56.7% and 1.7% isolates, respectively, in either the more frequent serotypes, S. 4,5:i:- and S. Typhimurium, and the more uncommon, S. Brandenburg and S. Infantis. MLVA and WGS allowed the identification of national and multinational outbreaks of S. Enteritidis. Although overall incidence rates of Salmonella did not change over time, trends and epidemiological factors differed remarkably by serotype. Indeed, there was a significantly increase of S. Enteritidis isolates during this period (2014 – 15.9%; 2015 - 25.0%; 2016 - 31.6%; 2017 - 37.1%). Continued surveillance of susceptibility is important due to the risk of resistance, particularly to fluoroquinolones and 3th and 4th generation cephalosporin’s. A better understanding of Salmonella epidemiology will assist in responding to this disease and in planning and implementing prevention activities. N/A
- Published
- 2018
16. Caracterização fenotípica de isolados de Shigella spp. entre 2015 e 2017
- Author
-
Silveira, Leonor, Pista, Ângela, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Portugal ,Shigella spp ,Caracterização Fenotípica - Abstract
Shigella spp. é uma enterobactéria altamente infeciosa e transmitida de pessoa-a-pessoa. As infeções por Shigella são um importante problema de Saúde Pública, em particular nos países em desenvolvimento. Em Portugal, a shigelose é uma causa rara de gastroenterite. A Sh.sonnei é mais frequente na Europa e EUA e a espécie flexneri na Ásia e África. O aparecimento de espécies multirresistentes tem sido referido mundialmente, com destaque para a resistência às fluoroquinolonas e cefalosporinas. Com este estudo pretendeu-se descrever os serotipos de Shigella identificados no Laboratório Nacional de Referência de Infeções Gastrintestinais do Instituto Nacional de Saúde Doutor Ricardo Jorge (INSA) entre 2015 e novembro de 2017. Foram analisadas 53 estirpes clínicas, isoladas a nível nacional e enviadas ao INSA para serotipagem. A suscetibilidade aos antimicrobianos foi realizada segundo as recomendações da EUCAST. Os serotipos mais frequentes foram Sh. sonnei (69,8%), Sh. flexneri 2 (13,2%), Sh. flexneri 3 (5,6%), Sh. flexneri 1 (3,8%), Sh. boydii 5 (3,8%), Sh. boydii 2 (1,9%) e Sh.dysenteriae 3 (1,9%). A idade dos pacientes variou entre os 1 e os 66 anos. Todas as estirpes foram resistentes a pelo menos um antibiótico. Foi observada uma elevada percentagem de resistência à tetraciclina (88,7%). O perfil de resistência mais frequente (77,4%) foi tetraciclina – trimetoprim - sulfametoxazole. A resistência às fluoroquinolonas e às cefalosporinas de 3ª e 4ª geração foi observada em, respetivamente, 22,6% e 1,9% das estirpes. Foi detetado um caso de Sh. sonnei multirresistente à ampicilina – tetraciclina – cefotaxime – ceftriaxone - ciprofloxacina - ácido nalidixico - trimetoprim-sulfametoxazole, num homem que faz sexo com homens (HSH). O serotipo mais frequente no período entre 2015 e novembro de 2017 foi Sh.sonnei, o que está de acordo com a tendência europeia. Apesar de não terem sido detetados muitos casos de resistência às fluoroquinolonas e cefalosporinas, estas não devem ser descuradas, uma vez que a sua emergência é evidente, em particular no caso dos HSH. Neste contexto, é fundamental manter e promover a colaboração entre os diversos Serviços de Saúde nacionais. N/A
- Published
- 2018
17. Salmonella enterica: serotipos e resistência aos antimicrobianos de isolados clínicos entre 2014 e 2017
- Author
-
Silveira, Leonor, Pista, Ângela, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Portugal ,Resistência aos Antimicrobianos ,Salmonella enterica ,Serotipos - Abstract
As infeções por Salmonella enterica são uma das causas mais frequentes de gastroenterite aguda em todo o mundo. A transmissão ocorre principalmente através da ingestão de água ou alimentos contaminados, mas também pode ocorrer por contacto direto com animais. Os sintomas são na maioria dos casos ligeiros, embora algumas formas invasivas de salmonelose possam conduzir a situações clínicas graves. Com este estudo pretendeu-se descrever os serotipos de S. enterica que foram identificados no Laboratório Nacional de Referência de Infeções Gastrintestinais do Instituto Nacional de Saúde Doutor Ricardo Jorge (INSA) entre 2014 e novembro de 2017. Foram analisadas 1082 estirpes clínicas, isoladas a nível nacional e enviadas ao INSA para serotipagem, de acordo com o esquema de classificação Kauffmann-White-de Minor. A suscetibilidade aos antimicrobianos foi realizada segundo as recomendações da EUCAST. Sempre que necessário, nomeadamente para identificação de surtos, foram utilizadas metodologias de MLVA e NGS. Das estirpes recebidas, 83,0% foram isoladas a partir de fezes, 10,8% de sangue, 2,1% de fezes e sangue e 4,1% de outros produtos biológicos. Foram identificados 55 serotipos. Os cinco mais frequentes foram S.4,5:i:- (33,6%), S.Enteritidis (29,3%), S.Typhimurium (18,4%), S.Rissen (2,3%), e S.Typhi (2,3%), representando 85,9% dos isolados estudados. Todos os casos de S.Typhi foram adquiridos em zonas endémicas. A resistência a antimicrobianos foi observada tanto nos serótipos mais frequentes (S.4,5:i:-, S.Typhimurium) como nos serótipos raros (S.Brandenburg, S.Infantis). A resistência a pelo menos um antibiótico foi observada em 56,7% estirpes e a quatro ou mais antibióticos em 1,7%. A utilização de MLVA e NGS permitiu a identificação de surtos de S.Enteritidis. Globalmente, os serotipos identificados mantiveram o perfil anteriormente observado e acompanham a tendência mundial. No entanto, houve um aumento significativo do número de casos de infeção por S.Enteritidis durante este período (2014-15,9%; 2015-25,0%; 201-31,6%; 2017-37,1%). A serotipagem e a utilização mais alargada das metodologias moleculares já disponíveis são fundamentais para a monitorização das salmoneloses humanas, nomeadamente na identificação de serotipos raros ou nunca detetados, de estirpes resistentes e de surtos. Neste contexto, é fundamental manter e promover a colaboração entre os diversos Serviços de Saúde nacionais. N/A
- Published
- 2018
18. Caracterização fenotípica e genotípica de isolados de Salmonella enterica recebidos no INSA entre 2014 e 2016
- Author
-
Silveira, Leonor, Pista, Ângela, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Portugal ,Salmonella enterica - Abstract
Caracterização fenotípica e genotípica de isolados de Salmonella enterica recebidos no INSA entre 2014 e 2016 N/A
- Published
- 2017
19. Caracterização fenotípica de isolados de Shigella spp. entre 2015 e 2016
- Author
-
Silveira, Leonor, Pista, Ângela, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Shigella spp - Abstract
Caracterização fenotípica de isolados de Shigella spp. entre 2015 e 2016 N/A
- Published
- 2017
20. Caracterização molecular e suscetibilidade aos antimicrobianos de isolados de L. monocytogenes na região de LVT em 2015
- Author
-
Silveira, Leonor, Pista, Ângela, Maia, Carla, Barreira, Maria João, Rodrigues, João, Reis, Lúcia, and Machado, Jorge
- Subjects
L. monocytogenes ,Infecções Gastrointestinais ,Segurança Alimentar ,Portugal ,Antimicrobianos - Abstract
Caracterização molecular e suscetibilidade aos antimicrobianos de isolados de L. monocytogenes na região de LVT em 2015. info:eu-repo/semantics/publishedVersion
- Published
- 2017
21. Molecular characterization and antimicrobial susceptibility of clinical isolates of Listeria monocytogenes in the Lisbon and Tagus Valley Region in 2015
- Author
-
Silveira, Leonor, Pista, Ângela, Maia, Carla, Barreira, Maria João, Rodrigues, João, Reis, Lúcia, and Machado, Jorge
- Subjects
Infecções Gastrointestinais ,Suscetibilidade aos Antimicrobianos ,Portugal ,Resistência aos Antimicrobianos ,Lisboa e Vale do Tejo ,Listeriose ,Caraterização Molecular ,Saúde Pública ,Listeria monocytogenes ,Vigilância Laboratorial - Abstract
Listeria monocytogenes é o agente causal da listeriose, uma doença grave considerada de vigilância prioritária a nível europeu, com taxas de hospitalização (98,9%) e de letalidade (15,0%) elevadas. Este estudo teve como objetivo a caraterização das estirpes de L. monocytogenes recebidas no Instituto Nacional de Saúde Doutor Ricardo Jorge em 2015. A serotipagem foi realizada por PCR multiplex, a tipagem por PFGE e também foi estudada a suscetibilidade aos antimicrobianos. O serotipo mais comum foi IVb (85,7%). A maioria das estirpes é sensível aos antimicrobianos em apreciação, com exceção de uma estirpe que apresentou resistência ao meropenemo e outra que apresentou resistência à eritromicina e ao trimetoprim/sulfametoxazol. Existe uma grande heterogeneidade de perfis de PFGE entre os isolados estudados, tendo sido detetados alguns clusters. A utilização de sequenciação de genoma completo para caraterização de estirpes, nomeadamente de L. monocytogenes está a ganhar terreno a nível mundial, vindo substituir as técnicas goldstandard. A sua aplicação no Laboratório Nacional de Referência de Infeções Gastrintestinais permitiu em 2015 a confirmação laboratorial de um surto. Listeria monocytogenes is the listeriosis agent, a serious disease under priority surveillance in Europe, with high mortality (15.0%) and hospitalization rates (98.9%). This study aims to characterize the Listeria monocytogenes strains received in the National Reference Laboratory of Gastrointestinal Infections of the National Institute of Health Dr. Ricardo Jorge in 2015. Serotyping was accomplished by multiplex PCR, typing by PFGE and the antimicrobial susceptibility was evaluated. The most common serotype was IVb (85.7%). Most strains are sensitive to the antimicrobials under consideration, with the exception of a strain that was resistant to meropenem and other that was resistant to erythromycin and trimethoprim/sulfamethoxazole. There is great heterogeneity between the PFGE profiles of the isolates under study, and some clusters have been detected. The use of whole sequencing for the characterization of strains namely L. monocytogenes surveillance is gaining ground worldwide, replacing the goldstandard techniques. Its application in the National Reference Laboratory of Gastrointestinal Infections allowed the effective resolution of an outbreak in 2015.
- Published
- 2016
22. High Prevalence of Human Papillomavirus on Anal and Oral Samples from Men and Women with External Anogenital Warts: The HERCOLES Study.
- Author
-
LISBOA, Carmen, SANTO, Irene, AZEVEDO, Jacinta, AZEVEDO, Luís, PISTA, Ângela, DIAS, Camila, and CUNHA, Maria João
- Subjects
GENITAL warts ,WARTS ,HETEROSEXUAL men ,PAPILLOMAVIRUSES ,BIOMARKERS ,DISEASE prevalence ,ANUS - Abstract
Human papillomavirus (HPV) infection is highly prevalent in the sexually active population. This study estimates the prevalence of HPV DNA in anal and oral samples from a cohort of men and women with incident anogenital warts. Anal and/or oral samples from 541 patients with anogenital warts were tested for 35 HPV genotypes using a PCR assay. The overall prevalence of anal HPV and oral HPV DNA was 59.9% (n = 305/509; 95% confidence interval (CI) 55.6-64.1%) and 14.5% (n = 78/538; 95% CI 11.8-17.7%), respectively. Among patients with perianal warts, the anal HPV DNA prevalence was 92.3% (95% CI 87.0-95.5%). Anal HPV DNA prevalence in patients with genital warts but no perianal warts was 55.7% (95% CI 50.6-60.7%). Both anal and oral HPV infections were more common in men who have sex with men than in heterosexual men (90.4% versus 38.5% and 20.8% versus 11.8%, respectively). Anal high risk-HPV infection was more common in women (58.8%) and in men who have sex with men (67.7%). We found that anogenital warts represent a clinical marker for both anal and oral HPV infections, including anal high risk-HPV infections, particularly among women and men who have sex with men. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
23. Use of the NucliSENS EasyQHPV assay in the management of cervical intraepithelial neoplasia
- Author
-
Oliveira, Ana, primary, Verdasca, Nuno, additional, and Pista, Ângela, additional
- Published
- 2013
- Full Text
- View/download PDF
24. Use of the Nucli SENS Easy Q HPV assay in the management of cervical intraepithelial neoplasia.
- Author
-
Oliveira, Ana, Verdasca, Nuno, and Pista, Ângela
- Abstract
Persistent infection by high-risk human papillomavirus is a necessary cause for cervical cancer. DNA-based human papillomavirus (HPV) assays show high sensitivity but poor specificity in detecting high-grade cervical lesions. Assays detecting mRNA of the oncoproteins E6 and E7 show higher specificity but lack either detection of all high-risk genotypes or the ability to specify the detected genotypes. The aim of this study was to evaluate the clinical performance of the NucliSENS EasyQ HPV assay in comparison with the Hybrid Capture 2 test (HC2) and the CLART Human Papillomavirus 2 assay (CLART), using a clinical cut-off of cervical intraepithelial neoplasia grade 2 or worse. In the 554 studied women, the lowest HPV positivity rate was detected for NucliSENS EasyQ HPV assay (55.1%), while HC2 and CLART showed similar results (HC2: 77.4%; CLART: 78.0%). In comparison with the other tests, the NucliSENS EasyQ HPV assay showed a lower clinical sensitivity (79.3% vs. 96.4% for HC2 and 95.9% for CLART) but a higher clinical specificity (72.6% vs. 42.8% for HC2 and 42.5% for CLART). Detection of E6/E7 mRNA transcripts may provide a higher specificity for cervical intraepithelial neoplasia grade 2 lesions or worse, since the oncogenic potential of HPV infection depends on the over-expression of these two oncoproteins. J. Med. Virol. 85:1235-1241, 2013. © 2013 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
25. Infections of the central nervous system associated with AIDS: Clinical syndromes of probably JCV aetiology.
- Author
-
Verdasca, Nuno, Pista, Ângela, Ribeiro, Fátima, and Paixão, M. T.
- Subjects
- *
INFECTION , *CENTRAL nervous system , *AIDS , *VIRUSES , *LATENT infection , *KIDNEY diseases , *DNA viruses - Abstract
Evaluates the infections of the central nervous system associated with AIDS and clinical syndromes of probably JC virus aetiology. Persistence of the latent virus infection in the kidney; Detection of the JC virus DNA in urine; Migration of the JC virus to the brain.
- Published
- 2003
26. Conhecimento das jovens acerca da infeção genital por HPV : um estudo piloto
- Author
-
Varino, Vanessa Elisabete Caboz Henriques Ribeiro, Nunes, Carla, and Pista, Ângela
- Subjects
Knowledge ,Jovens ,Infeção por HPV ,Conhecimento ,Teenager ,HPV Infection - Abstract
RESUMO - A infeção por Vírus do Papiloma Humano (HPV) constitui uma das infeções de transmissão sexual mais comuns em mulheres adolescentes e jovens sexualmente ativas, sendo responsável por 99,7% dos casos de cancro do colo do útero (CCU). O desenvolvimento de duas vacinas de segurança e eficácia comprovadas e a introdução da vacinação contra os HPV 6, 11, 16 e 18 nos programas de imunização de diversos países constituiu um marco muito importante para a investigação no âmbito do conhecimento das populações nesta temática. O presente trabalho de projeto teve como objetivo geral avaliar o conhecimento dos adolescentes/jovens entre os 15 e os 19 anos acerca da infeção genital por HPV. Foi efetuado um estudo piloto cuja amostra foi constituída por 20 jovens, género feminino, que acorreram à Consulta de Obstetrícia e Ginecologia do Adolescente dos Serviços de Assistência Médico-Social do Sindicato dos Bancários do Sul e Ilhas, acompanhadas dos respetivos encarregados de educação. Foi-lhes solicitado o consentimento informado e entregue um questionário de caráter anónimo e confidencial com questões para avaliação do conhecimento geral acerca da infeção por HPV, formas de transmissão, manifestações clínicas, prevenção, relação entre HPV e CCU e necessidades de informação. Este estudo piloto poder-se-á revelar útil na aferição de resultados quando aplicado em larga escala, para posterior avaliação comparativa do conhecimento no âmbito do HPV. Pode igualmente ser útil no desenvolvimento de mensagens apropriadas que acompanham os programas de rastreio nesta coorte, no futuro, com vista ao aumento da literacianos jovens. ABSTRACT - Infection with Human Papillomavirus (HPV) is one of the most common sexually transmitted infections in sexually active adolescents and young women and it is a risk factor for approximately 99,7% of all cases of cervical cancer globally. The recent development of two safe and clinically effective vaccines against HPV’s 6, 11, 16 e 18 and the introduction of this vaccines in the immunity programs of many countries was a promising step for investigation in this area. The aim of this project work was to assess adolescents/young people knowledge of HPV genital infection. A pilot study was conducted in a sample of 20 adolescents/young people aged 15 – 19 years seen at the Obstetric and Ginecology Adolescent Consulting at Medico-Social Assistant Services at Sindicato dos Bancários do Sul e Ilhas (bank workers syndicate). A self-administered anonymous questionnaire covered general knowledge about HPV infection, transmission, prevention, HPV relationship with cervical cancer and identifying problems with information provision. This study pilot could be helpful in benchmarking HPV related knowledge and could be used in the development of appropriate educational messages to accompany the screening programs in this cohort in the future, with a goal of increasing healt literacy in young people.
- Published
- 2013
27. Proporção dos diferentes tipos de Vírus do Papiloma Humano no colo do útero de mulheres infectadas pelo Vírus da Imunodeficiência Humana
- Author
-
Bernardino, Margarida Neto Vilhena, Paixão, Maria Teresa d’Avillez, and Pista, Ângela Maria Chambel
- Abstract
Submitted by Agostinho Macau (arm@lisboa.ucp.pt) on 2012-05-11T09:07:48Z No. of bitstreams: 1 tese margarida bernardino.pdf: 754040 bytes, checksum: c532bb0773637d0ff8b8485165d39162 (MD5) Approved for entry into archive by Agostinho Macau(arm@lisboa.ucp.pt) on 2012-05-11T09:08:45Z (GMT) No. of bitstreams: 1 tese margarida bernardino.pdf: 754040 bytes, checksum: c532bb0773637d0ff8b8485165d39162 (MD5) Made available in DSpace on 2012-05-11T09:08:45Z (GMT). No. of bitstreams: 1 tese margarida bernardino.pdf: 754040 bytes, checksum: c532bb0773637d0ff8b8485165d39162 (MD5) Previous issue date: 2011
- Published
- 2011
28. Novas abordagens para o rastreio do cancro do colo do útero e para a prática clínica
- Author
-
Marta Costa Neto, Pista, Ângela Maria Chambel, and Tenreiro, Rogério Paulo de Andrade
- Subjects
Papillomavírus humano ,Cancro do colo do útero ,Teses de mestrado ,Biologia molecular - Abstract
Tese de mestrado, Biologia (Microbiologia Aplicada), 2009, Universidade de Lisboa, Faculdade de Ciências Nível de preservação: critical, Criado em: 2010-03-19 18:30:05, Criado por: creator:LSARAM, Alterado em: 2010-03-23 15:45:47, Modificado por: super:LSARAM Made available in DSpace on 2010-07-28T16:13:18Z (GMT). No. of bitstreams: 2 21619_ulfc080739_tm.pdf: 552592 bytes, checksum: fba67112671446680bcce20f34020a99 (MD5) 21619.xml: 9805 bytes, checksum: 035f4fe18da522cb23a34475d1092a06 (MD5) Previous issue date: 2009
- Published
- 2009
29. Desenvolvimento de metodologias de diagnóstico e caracterização molecular de Poliomavírus
- Author
-
Ferreira, Rita Susana Da Silva, Pista, Ângela Maria Chambel, and Caeiro, Maria Filomena Ribeiro Alcobia da Silva Trabucho, 1950
- Subjects
Poliomavírus ,Microbiologia ,Teses de mestrado - 2009 ,Biologia molecular - Abstract
Tese de mestrado. Biologia (Biologia Molecular Humana). Universidade de Lisboa, Faculdade de Ciências, 2009 Submitted by Lurdes Saramago (lurdes.saramago@fc.ul.pt) on 2011-05-12T16:56:01Z No. of bitstreams: 1 ulfc_tm_rita_ferreira.pdf: 1545688 bytes, checksum: e0655d8d377ac4eff561d2497c028ffb (MD5) Made available in DSpace on 2011-05-12T16:56:49Z (GMT). No. of bitstreams: 1 ulfc_tm_rita_ferreira.pdf: 1545688 bytes, checksum: e0655d8d377ac4eff561d2497c028ffb (MD5) Previous issue date: 2009
- Published
- 2009
30. Implementation and evaluation of new methodologies for early diagnosis of high risk Human Papillomavirus Infection
- Author
-
Delgado, Cândida Filipa Abreu Castro, Pista, Ângela Maria Chambel, and Paulo, Octávio
- Subjects
Papillomavírus humano ,Teses de mestrado ,Biologia molecular - Abstract
Tese de mestrado, Biologia (Biologia Humana e Ambiente), 2009, Universidade de Lisboa, Faculdade de Ciências Nível de preservação: critical, Criado em: 2010-01-06 14:19:53, Criado por: creator:LSARAM, Alterado em: 2010-01-06 15:32:17, Modificado por: super:LSARAM Made available in DSpace on 2010-07-27T08:58:14Z (GMT). No. of bitstreams: 2 20672_ulfc080616_tm.pdf: 2310213 bytes, checksum: 166cbf7a1509b016cd294ddabe71b689 (MD5) 20672.xml: 8440 bytes, checksum: f50416ef6a807d9f8ad15d5c2ff7b779 (MD5) Previous issue date: 2009
- Published
- 2009
31. Salmonella Saintpaul outbreak associated with cantaloupe consumption, the United Kingdom and Portugal, September to November 2023.
- Author
-
McGeoch LJ, Hoban A, Sawyer C, Rabie H, Painset A, Browning L, Brown D, McCarthy C, Nelson A, Firme A, Pista Â, Moreno J, Martins JV, Silveira L, Machado J, Vasconcelos P, Olufon O, Inzoungou-Massanga C, Douglas A, McCormick J, Larkin L, and Balasegaram S
- Subjects
- Humans, Portugal epidemiology, Male, Adult, Female, United Kingdom epidemiology, Middle Aged, Child, Adolescent, Case-Control Studies, Young Adult, Aged, Child, Preschool, Cucumis melo microbiology, Salmonella genetics, Salmonella isolation & purification, Salmonella classification, Infant, Aged, 80 and over, Phylogeny, Disease Outbreaks, Salmonella Food Poisoning epidemiology, Salmonella Food Poisoning microbiology
- Abstract
In September 2023, the UK Health Security Agency identified cases of Salmonella Saintpaul distributed across England, Scotland, and Wales, all with very low genetic diversity. Additional cases were identified in Portugal following an alert raised by the United Kingdom. Ninety-eight cases with a similar genetic sequence were identified, 93 in the United Kingdom and 5 in Portugal, of which 46% were aged under 10 years. Cases formed a phylogenetic cluster with a maximum distance of six single nucleotide polymorphisms (SNPs) and average of less than one SNP between isolates. An outbreak investigation was undertaken, including a case-control study. Among the 25 UK cases included in this study, 13 reported blood in stool and 5 were hospitalized. One hundred controls were recruited via a market research panel using frequency matching for age. Multivariable logistic regression analysis of food exposures in cases and controls identified a strong association with cantaloupe consumption (adjusted odds ratio: 14.22; 95% confidence interval: 2.83-71.43; p -value: 0.001). This outbreak, together with other recent national and international incidents, points to an increase in identifications of large outbreaks of Salmonella linked to melon consumption. We recommend detailed questioning and triangulation of information sources to delineate consumption of specific fruit varieties during Salmonella outbreaks.
- Published
- 2024
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.