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82 results on '"Piscitelli, C."'

1. Construction and test of the SM1 type Micromegas chambers for the upgrade of the ATLAS forward muon spectrometer

Author

Agarwala, J., Alviggi, M.G., Antonelli, M., Anulli, F., Arcangeletti, C., Auricchio, S., Bagnaia, P., Bariani, S., Baroncelli, A., Bashta, I., Bauce, M., Beretta, M., Bini, C., Calabró, D., Camerlingo, M.T., Canale, V., Capitolo, E., Capponi, M., Capradossi, G., Carducci, G., Caserio, A., Cassese, C., Cerioni, S., Chiarella, V., Ciapetti, G., D’Amico, V., De Cecco, S., De Fazio, B., Del Gaudio, M., Di Donato, C., Di Nardo, R., D’Uffizi, D., Farilla, A., Farina, E., Ferrari, R., Fiore, G., Frattari, G., Freddi, A., Gaudio, G., Gauzzi, P., Giagu, S., Gigli, S., Gnesi, I., Gorini, E., Gravili, F.G., Greco, M., Iaciofano, A., Iengo, P., Innocente, A., Introzzi, G., Iodice, M., Ippolito, V., Kourkoumeli Charalampidi, A., Lacava, F., Lalli, E., Lanza, A., Lauciani, S., Laurelli, P., Luminari, L., Mancini, G., Martinelli, L., Massarotti, P., Miccoli, A., Mirto, A., Negri, A., Orestano, D., Paruzza, G., Pepe, M., Petruccetti, M., Petrucci, F., Pezzotti, L., Pileggi, G., Pirola, M., Piscitelli, C., Polesello, G., Policicchio, A., Pontoriere, G., Ponzio, B., Primavera, M., Rebuzzi, D., Rimoldi, A., Romano, E., Roscilli, L., Rovelli, G., Russo, V., Sansoni, A., Safai Tehrani, F., Scagliotti, C., Schioppa, M., Sekhniaidze, G., Sessa, M., Sottocornola, S., Trattino, P., Tskhadadze, E., Turco, P., Vannicola, D., Vannoli, L., Vassilieva, T., Vecchio, V., Ventura, A., Vercellati, F., Vilucchi, E., Zullo, A., and Zunica, G.

Published
2022
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2. Introducing the speech therapist in the Neonatology Unit: an innovative way to support breastfeeding in healthy newborns even during COVID-19 pandemic

Author

Maragliano, G., primary, Piscitelli, C., additional, Iorio, C., additional, Cavaliere, M., additional, Pinna, G., additional, Carucci, T., additional, Cao, M., additional, De Nardo, M.C., additional, Clemente, A., additional, Guadagno, C., additional, Di Feliciantonio, M., additional, and Barletta, F., additional

Published
2023
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3. Construction and test of the SM1 type Micromegas chambers for the upgrade of the ATLAS forward muon spectrometer

Author

Agarwala J., Alviggi M. G., Antonelli M., Anulli F., Arcangeletti C., Auricchio S., Bagnaia P., Bariani S., Baroncelli A., Bashta I., Bauce M., Beretta M., Bini C., Calabro D., Camerlingo M. T., Canale V., Capitolo E., Capponi M., Capradossi G., Carducci G., Caserio A., Cassese C., Cerioni S., Chiarella V., Ciapetti G., D'Amico V., De Cecco S., De Fazio B., Del Gaudio M., Di Donato C., Di Nardo R., D'Uffizi D., Farilla A., Farina E., Ferrari R., Fiore G., Frattari G., Freddi A., Gaudio G., Gauzzi P., Giagu S., Gigli S., Gnesi I., Gorini E., Gravili F. G., Greco M., Iaciofano A., Iengo P., Innocente A., Introzzi G., Iodice M., Ippolito V., Kourkoumeli Charalampidi A., Lacava F., Lalli E., Lanza A., Lauciani S., Laurelli P., Luminari L., Mancini G., Martinelli L., Massarotti P., Miccoli A., Mirto A., Negri A., Orestano D., Paruzza G., Pepe M., Petruccetti M., Petrucci F., Pezzotti L., Pileggi G., Pirola M., Piscitelli C., Polesello G., Policicchio A., Pontoriere G., Ponzio B., Primavera M., Rebuzzi D., Rimoldi A., Romano E., Roscilli L., Rovelli G., Russo V., Sansoni A., Safai Tehrani F., Scagliotti C., Schioppa M., Sekhniaidze G., Sessa M., Sottocornola S., Trattino P., Tskhadadze E., Turco P., Vannicola D., Vannoli L., Vassilieva T., Vecchio V., Ventura A., Vercellati F., Vilucchi E., Zullo A., Zunica G., Agarwala, J., Alviggi, M. G., Antonelli, M., Anulli, F., Arcangeletti, C., Auricchio, S., Bagnaia, P., Bariani, S., Baroncelli, A., Bashta, I., Bauce, M., Beretta, M., Bini, C., Calabro, D., Camerlingo, M. T., Canale, V., Capitolo, E., Capponi, M., Capradossi, G., Carducci, G., Caserio, A., Cassese, C., Cerioni, S., Chiarella, V., Ciapetti, G., D'Amico, V., De Cecco, S., De Fazio, B., Del Gaudio, M., Di Donato, C., Di Nardo, R., D'Uffizi, D., Farilla, A., Farina, E., Ferrari, R., Fiore, G., Frattari, G., Freddi, A., Gaudio, G., Gauzzi, P., Giagu, S., Gigli, S., Gnesi, I., Gorini, E., Gravili, F. G., Greco, M., Iaciofano, A., Iengo, P., Innocente, A., Introzzi, G., Iodice, M., Ippolito, V., Kourkoumeli Charalampidi, A., Lacava, F., Lalli, E., Lanza, A., Lauciani, S., Laurelli, P., Luminari, L., Mancini, G., Martinelli, L., Massarotti, P., Miccoli, A., Mirto, A., Negri, A., Orestano, D., Paruzza, G., Pepe, M., Petruccetti, M., Petrucci, F., Pezzotti, L., Pileggi, G., Pirola, M., Piscitelli, C., Polesello, G., Policicchio, A., Pontoriere, G., Ponzio, B., Primavera, M., Rebuzzi, D., Rimoldi, A., Romano, E., Roscilli, L., Rovelli, G., Russo, V., Sansoni, A., Safai Tehrani, F., Scagliotti, C., Schioppa, M., Sekhniaidze, G., Sessa, M., Sottocornola, S., Trattino, P., Tskhadadze, E., Turco, P., Vannicola, D., Vannoli, L., Vassilieva, T., Vecchio, V., Ventura, A., Vercellati, F., Vilucchi, E., Zullo, A., and Zunica, G.

Subjects
Nuclear and High Energy Physics, Muon detector, Micromegas, Instrumentation, LHC, ATLAS, High Energy Physics, Micromegas, Muons
Abstract

Large-size Resistive Micromegas have been chosen for the upgrade of the forward muon spectrometer of the ATLAS experiment, the New Small Wheel project. These chambers, together with small-strip Thin Gap Chambers (sTGC), allow reconstruction of high-momentum muon tracks in a high-radiation environment and provide a robust low-threshold single-muon trigger. A collaboration of seven INFN units built 32 SM1 type chambers, corresponding to one fourth of the total number needed for this upgrade. Each SM1 chamber has a surface of approximately 2 m2 and four sensitive layers. The production was shared among five INFN construction sites and it was completed in fall 2020. The construction methods, as well as the results of the quality tests done on components of the detector and on the assembled chambers, are reported in the present paper.

Published
2022

4. Tomato plants (Lycopersicon esculentum L.) grown in experimental contaminated soil: bioconcentration of potentially toxic elements and free radical scavenging evaluation

Author

Piscitelli C., Lavorgna M., De Prisco R., Coppola E., Grilli E., Russo C., Isidori M., Piscitelli, C., Lavorgna, M., De Prisco, R., Coppola, E., Grilli, E., Russo, C., and Isidori, M.

Subjects
soil pollutant, antioxidant activity, translocation, heavy metal, Lycopersicon esculentum, PTE
Abstract

Tomato is the most widespread vegetable crop in the world. In Italy, tomatoes are mainly cultivated in the South and in the Campania region, precisely in the area called Agro Nocerino- Sarnese. This flatland is affected by an extreme level of environmental degradation, especially related to the Sarno River, where concentrations of Potential Toxic Elements (PTEs) have been found to be higher than the maximum permitted level. The aim of this study was to determine the PTEs uptake by roots and their translocation to the aerial parts of the plants of two cultivars of tomatoes (Pomodoro Giallo and San Marzano Cirio 3). To the purpose, samples of the two cultivars were grown both in pots with experimentally contaminated soil containing: Cr or Cd or Pb at extremely high concentrations and in pots with uncontaminated soils (control). Additionally, the antioxidant properties of the cultivars selected grown on uncontaminated/contaminated soils were assessed. The results showed that Cd was the contaminant that most significantly interfered with the growth of both cultivars of tomato plants, whereas Pb caused lower phenotypical damage. Cd translocation from root to the organs of tomato plants was observed in both cultivars. Specifically, the total amount of Cd found in stems and leaves was higher in the Pomodoro Giallo (254.4 mg/ kg dry weight) than in the San Marzano Cirio 3 (165.8 mg/kg dry weight). Cd was the only PTE found in the fruits of both cultivars, with values of 6.1 and 3.9 mg/kg dry weight of Pomodoro Giallo and San Marzano Cirio 3, respectively. The fruits of tomato plants grown in PTEs-contaminated soil showed inhibition or stimulations of the radical scavenging activity compared to the fruits grown in uncontaminated soil. This study highlighted that, despite the relatively high experimental concentrations of PTEs, their translocation to the edible part was comparatively low or absent.

Published
2020

5. Construction of the inner layer barrel drift chambers of the ATLAS muon spectrometer at the LHC

Author

Bagnaia, P., Barisonzi, M., Bini, C., Calabrò, D., Caloi, R., Cambiaghi, M., Capradossi, G., Cavallari, A., Cecconi, V., Ciapetti, G., Daly, C.H., De Salvo, A., De Zorzi, G., Di Domenico, A., Di Mattia, A., Ferrari, R., Fraternali, M., Freddi, A., Gaudio, G., Gauzzi, P., Gentile, S., Iannone, M., Iuvino, G., Lacava, F., Lanza, A., Livan, M., Lubatti, H.J., Luci, C., Mattei, A., Nardoni, C., Negri, A., Pelosi, A., Piscitelli, C., Pontecorvo, L., Rebuzzi, D., Scagliotti, C., Scannicchio, D.A., Valente, P., Vercellati, F., and Zanello, L.

Published
2005
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19. mRNA processing and natural polarity in the histidine operon

Author

ALIFANO, Pietro, RIVELLINI F, NAPPO A. G, PISCITELLI C, BLASI V, PICONE M, CARLOMAGNO M. S., Alifano, Pietro, Rivellini, F, NAPPO A., G, Piscitelli, C, Blasi, V, Picone, M, and Carlomagno, M. S.

Abstract

published on J. Cell. Biochem., Supplement 16E.

Published
1992

26. ETHICS AND LAW

Author

Cattoli, M., primary, Bianchi, V., additional, Bonu, M. A., additional, Sciajno, R., additional, Lappi, M., additional, Borini, A., additional, Rubino, P., additional, Cotarelo, R. P., additional, Alteri, A., additional, Antinori, E., additional, Colicchia, A., additional, Giannini, P., additional, Piscitelli, C., additional, Greco, E., additional, Oudi, M., additional, Omani Samani, R., additional, Bellavia, M., additional, Wunder, D., additional, Bay, B., additional, Bruun-Petersen, G., additional, Guzoglu, N., additional, Kanmaz, H. G., additional, Uras, N., additional, Erdeve, O., additional, Dilli, D., additional, and Dilmen, U., additional

Published
2012
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27. POSTER VIEWING SESSION - EMBRYOLOGY

Author

Fourati Ben Mustapha, S., primary, Khrouf, M., additional, Kacem Ben Rejeb, K., additional, Elloumi Chaabene, H., additional, Merdassi, G., additional, Wahbi, D., additional, Ben Meftah, M., additional, Zhioua, F., additional, Zhioua, A., additional, Azzarello, A., additional, Host, T., additional, Mikkelsen, A. L., additional, Theofanakis, C. P., additional, Dinopoulou, V., additional, Mavrogianni, D., additional, Partsinevelos, G. A., additional, Drakakis, P., additional, Stefanidis, K., additional, Bletsa, A., additional, Loutradis, D., additional, Rienzi, L., additional, Cobo, A., additional, Paffoni, A., additional, Scarduelli, C., additional, Capalbo, A., additional, Garrido, N., additional, Remohi, J., additional, Ragni, G., additional, Ubaldi, F. M., additional, Herrer, R., additional, Quera, M., additional, GIL, E., additional, Serna, J., additional, Grondahl, M. L., additional, Bogstad, J., additional, Agerholm, I. E., additional, Lemmen, J. G., additional, Bentin-Ley, U., additional, Lundstrom, P., additional, Kesmodel, U. S., additional, Raaschou-Jensen, M., additional, Ladelund, S., additional, Guzman, L., additional, Ortega, C., additional, Albuz, F. K., additional, Gilchrist, R. B., additional, Devroey, P., additional, Smitz, J., additional, De Vos, M., additional, Bielanska, M., additional, Leveille, M. C., additional, Borghi, E., additional, Magli, M. C., additional, Figueroa, M. J., additional, Mascaretti, G., additional, Ferraretti, A. P., additional, Gianaroli, L., additional, Szlit, E., additional, Leocata Nieto, F., additional, Maggiotto, G., additional, Arenas, G., additional, Tarducci Bonfiglio, N., additional, Ahumada, A., additional, Asch, R., additional, Sciorio, R., additional, Dayoub, N., additional, Thong, J., additional, Pickering, S., additional, Ten, J., additional, Carracedo, M. A., additional, Guerrero, J., additional, Rodriguez-Arnedo, A., additional, Llacer, J., additional, Bernabeu, R., additional, Tatone, C., additional, Heizenrieder, T., additional, Di Emidio, G., additional, Treffon, P., additional, Seidel, T., additional, Eichenlaub-Ritter, U., additional, Cortezzi, S. S., additional, Cabral, E. C., additional, Ferreira, C. R., additional, Trevisan, M. G., additional, Figueira, R. C. S., additional, Braga, D. P. A. F., additional, Eberlin, M. N., additional, Iaconelli Jr., A., additional, Borges Jr., E., additional, Zabala, A., additional, Pessino, T., additional, Blanco, L., additional, Rey Valzacchi, G., additional, Leocata, F., additional, Vanden Meerschaut, F., additional, Heindryckx, B., additional, Qian, C., additional, Deforce, D., additional, Leybaert, L., additional, De Sutter, P., additional, De las Heras, M., additional, De Pablo, J. L., additional, Navarro, B., additional, Agirregoikoa, J. A., additional, Barrenetxea, G., additional, Cruz, M., additional, Perez-Cano, I., additional, Gadea, B., additional, Herrero, J., additional, Martinez, M., additional, Roldan, M., additional, Munoz, M., additional, Pellicer, A., additional, Meseguer, M., additional, Galindo, N., additional, Scarselli, F., additional, Alviggi, E., additional, Colasante, A., additional, Minasi, M. G., additional, Rubino, P., additional, Lobascio, M., additional, Ferrero, S., additional, Litwicka, K., additional, Varricchio, M. T., additional, Giannini, P., additional, Piscitelli, P., additional, Franco, G., additional, Zavaglia, D., additional, Nagy, Z. P., additional, Greco, E., additional, Urner, F., additional, Wirthner, D., additional, Murisier, F., additional, Mock, P., additional, Germond, M., additional, Amorocho Llanos, B., additional, Calderon, G., additional, Lopez, D., additional, Fernandez, L., additional, Nicolas, M., additional, Landeras, J., additional, Finn-Sell, S. L., additional, Leandri, R., additional, Fleming, T. P., additional, Macklon, N. S., additional, Cheong, Y. C., additional, Eckert, J. J., additional, Lee, J. H., additional, Jung, Y. J., additional, Hwang, H. K., additional, Kang, A., additional, An, S. J., additional, Jung, J. Y., additional, Kwon, H. C., additional, Lee, S. J., additional, Palini, S., additional, Zolla, L., additional, De Stefani, S., additional, Scala, V., additional, D'Alessandro, A., additional, Polli, V., additional, Rocchi, P., additional, Tiezzi, A., additional, Pelosi, E., additional, Dusi, L., additional, Bulletti, C., additional, Fadini, R., additional, Lain, M., additional, Mignini Renzini, M., additional, Brambillasca, F., additional, Coticchio, G., additional, Merola, M., additional, Guglielmo, M. C., additional, Dal Canto, M., additional, Figueira, R., additional, Setti, A. S., additional, Worrilow, K. C., additional, Uzochukwu, C. D., additional, Eid, S., additional, Le Gac, S., additional, Esteves, T. C., additional, van Rossem, F., additional, van den Berg, A., additional, Boiani, M., additional, Kasapi, E., additional, Panagiotidis, Y., additional, Goudakou, M., additional, Papatheodorou, A., additional, Pasadaki, T., additional, Prapas, N., additional, Prapas, Y., additional, Vanderzwalmen, P., additional, Norasing, S., additional, Atchajaroensatit, P., additional, Tawiwong, W., additional, Thepmanee, O., additional, Saenlao, S., additional, Aojanepong, J., additional, Hunsajarupan, P., additional, Sajjachareonpong, K., additional, Punyatanasakchai, P., additional, Maneepalviratn, S., additional, Jetsawangsri, U., additional, Tejera, A., additional, Rubio, I., additional, Romero, J. L., additional, Nordhoff, V., additional, Schlatt, S., additional, Schuring, A. N., additional, Kiesel, L., additional, Kliesch, S., additional, Azambuja, R., additional, Okada, L., additional, Lazzari, V., additional, Dorfman, L., additional, Michelon, J., additional, Badalotti, M., additional, Badalotti, F., additional, Petracco, A., additional, Schwarzer, C., additional, Versieren, K., additional, De Croo, I., additional, Lierman, S., additional, De Vos, W., additional, Van den Abbeel, E., additional, Gerris, J., additional, Milacic, I., additional, Borogovac, D., additional, Veljkovic, M., additional, Arsic, B., additional, Jovic Bojovic, D., additional, Lekic, D., additional, Pavlovic, D., additional, Garalejic, E., additional, Albertini, D. F., additional, De Ponti, E., additional, Sanges, F., additional, Talevi, R., additional, Papini, L., additional, Mollo, V., additional, Rienzi, L. F., additional, Gualtieri, R., additional, Orteg, C., additional, Choi, J., additional, Lee, H., additional, Ku, S., additional, Kim, S., additional, Choi, Y., additional, Kim, J., additional, Moon, S., additional, Demilly, E., additional, Assou, S., additional, Moussaddykine, S., additional, Dechaud, H., additional, Hamamah, S., additional, Takisawa, T., additional, Doshida, M., additional, Hattori, H., additional, Nakamura, Y., additional, Kyoya, T., additional, Shibuya, Y., additional, Nakajo, Y., additional, Tasaka, A., additional, Toya, M., additional, Kyono, K., additional, Novo, S., additional, Penon, O., additional, Gomez, R., additional, Barrios, L., additional, Duch, M., additional, Santalo, J., additional, Esteve, J., additional, Nogues, C., additional, Plaza, J. A., additional, Perez-Garcia, L., additional, Ibanez, E., additional, Chavez, S., additional, Loewke, K., additional, Behr, B., additional, Reijo Pera, R., additional, Huang, S., additional, Wang, H., additional, Soong, Y., additional, Chang, C., additional, Okimura, T., additional, Kuwayama, M., additional, Mori, C., additional, Morita, M., additional, Uchiyama, K., additional, Aono, F., additional, Kato, K., additional, Takehara, Y., additional, Kato, O., additional, Minasi, M., additional, Casciani, V., additional, Arizzi, L., additional, Mencacci, C., additional, Piscitelli, C., additional, Cucinelli, F., additional, Tocci, A., additional, Wydooghe, E., additional, Vandaele, L., additional, Dewulf, J., additional, Van Soom, A., additional, Moon, J. H., additional, Son, W. Y., additional, Mahfoudh, A., additional, Henderson, S., additional, Jin, S. G., additional, Shalom-Paz, E., additional, Dahan, M., additional, Holzer, H., additional, Mahmoud, K., additional, Triki-Hmam, C., additional, Terras, K., additional, Hfaiedh, T., additional, Ben Aribia, M. H., additional, Otsubo, H., additional, Egashira, A., additional, Tanaka, K., additional, Matsuguma, T., additional, Murakami, M., additional, Murakami, K., additional, Otsuka, M., additional, Yoshioka, N., additional, Araki, Y., additional, Kuramoto, T., additional, Smit, J. G., additional, Sterrenburg, M. D., additional, Eijkemans, M. J. C., additional, Al-Inany, H. G., additional, Youssef, M. A. F. M., additional, Broekmans, F. J. M., additional, Willoughby, K., additional, DiPaolo, L., additional, Deys, L., additional, Lagunov, A., additional, Amin, S., additional, Faghih, M., additional, Hughes, E., additional, Karnis, M., additional, Ashkar, F., additional, King, W. A., additional, Neal, M. S., additional, Antonova, I., additional, Veleva, L., additional, Petkova, L., additional, Shterev, A., additional, Nogales, C., additional, Martinez, E., additional, Ariza, M., additional, Cernuda, D., additional, Gaytan, M., additional, Linan, A., additional, Guillen, A., additional, Bronet, F., additional, Cottin, V., additional, Fabian, D., additional, Allemann, F., additional, Koller, A., additional, Spira, J. C., additional, Agudo, D., additional, Martinez-Burgos, M., additional, Arnanz, A., additional, Basile, N., additional, Rodriguez, A., additional, Cho, Y. S., additional, Filioli Uranio, M., additional, Ambruosi, B., additional, Paternoster, M. S., additional, Totaro, P., additional, Sardanelli, A. M., additional, Dell'Aquila, M. E., additional, Zollner, U., additional, Hofmann, T., additional, Zollner, K. P., additional, Kovacic, B., additional, Roglic, P., additional, Vlaisavljevic, V., additional, Sole, M., additional, Boada, M., additional, Coroleu, B., additional, Veiga, A., additional, Martiny, G., additional, Molinari, M., additional, Revelli, A., additional, Chimote, N. M., additional, Chimote, M., additional, Mehta, B., additional, Chimote, N. N., additional, Sheikh, N., additional, Nath, N., additional, Mukherjee, A., additional, Rakic, K., additional, Reljic, M., additional, Ingerslev, H. J., additional, Kirkegaard, K., additional, Hindkjaer, J., additional, Agerholm, I., additional, Kitasaka, H., additional, Fukunaga, N., additional, Nagai, R., additional, Yoshimura, T., additional, Tamura, F., additional, Kitamura, K., additional, Hasegawa, N., additional, Nakayama, K., additional, Katou, M., additional, Itoi, F., additional, Asano, E., additional, Deguchi, N., additional, Ooyama, K., additional, Hashiba, Y., additional, Asada, Y., additional, Michaeli, M., additional, Rotfarb, N., additional, Karchovsky, E., additional, Ruzov, O., additional, Atamny, R., additional, Slush, K., additional, Fainaru, O., additional, Ellenbogen, A., additional, Chekuri, S., additional, Chaisrisawatsuk, T., additional, Chen, P., additional, Pangestu, M., additional, Jansen, S., additional, Catt, S., additional, Molinari, E., additional, Racca, C., additional, Ryu, C., additional, Kang, S., additional, Lee, J., additional, Chung, D., additional, Roh, S., additional, Chi, H., additional, Yokota, Y., additional, Yokota, M., additional, Yokota, H., additional, Sato, S., additional, Nakagawa, M., additional, Komatsubara, M., additional, Makita, M., additional, Oyama, K., additional, Naruse, K., additional, Kilani, S., additional, Chapman, M. G., additional, Kwik, M., additional, Chapman, M., additional, Guven, S., additional, Odaci, E., additional, Yildirim, O., additional, Kart, C., additional, Unsal, M. A., additional, Yulug, E., additional, Isachenko, E., additional, Maettner, R., additional, Strehler, E., additional, Isachenko, V., additional, Hancke, K., additional, Kreienberg, R., additional, Sterzik, K., additional, Zheng, X. Y., additional, Wang, L. N., additional, Liu, P., additional, Qiao, J., additional, Inoue, F., additional, Dashtizad, M., additional, Wahid, H., additional, Rosnina, Y., additional, Daliri, M., additional, Hajarian, H., additional, Akbarpour, M., additional, Abbas Mazni, O., additional, Knez, K., additional, Tomaevic, T., additional, Vrtacnik Bokal, E., additional, Zorn, B., additional, Virant Klun, I., additional, Koster, M., additional, Liebenthron, J., additional, Nicolov, A., additional, van der Ven, K., additional, van der Ven, H., additional, Montag, M., additional, Fayazi, M., additional, Salehnia, M., additional, Beigi Boroujeni, M., additional, Khansarinejad, B., additional, Deignan, K., additional, Emerson, G., additional, Mocanu, E., additional, Wang, J. J., additional, Andonov, M., additional, Linara, E., additional, Ahuja, K. K., additional, Nachef, S., additional, Pasqualotto, F. F., additional, Pasqualotto, E., additional, Chang, C. C., additional, Bernal, D. P., additional, Elliott, T. A., additional, Shapiro, D. B., additional, Toledo, A. A., additional, Economou, K., additional, Davies, S., additional, Argyrou, M., additional, Doriza, S., additional, Sisi, P., additional, Moschopoulou, M., additional, Karagianni, A., additional, Mendorou, C., additional, Polidoropoulos, N., additional, Papanicopoulos, C., additional, Stefanis, P., additional, Karamalegos, C., additional, Cazlaris, H., additional, Koutsilieris, M., additional, Mastrominas, M., additional, Gotts, S., additional, Doshi, A., additional, Harper, J., additional, Serhal, P., additional, Borini, A., additional, Guzeloglu-Kayisli, O., additional, Bianchi, V., additional, Seli, E., additional, Lappi, M., additional, Bonu, M. A., additional, Mizuta, S., additional, Hashimoto, H., additional, Kuroda, Y., additional, Matsumoto, Y., additional, Mizusawa, Y., additional, Ogata, S., additional, Yamada, S., additional, Kokeguchi, S., additional, Noda, Y., additional, Shiotani, M., additional, Stojkovic, M., additional, Ilic, M., additional, Markovic, N., additional, Stojkovic, P., additional, Feng, G., additional, Zhang, B., additional, Zhou, H., additional, Zhou, L., additional, Gan, X., additional, Qin, X., additional, Shu, J., additional, Wu, F., additional, Molina Botella, I., additional, Lazaro Ibanez, E., additional, Debon Aucejo, A., additional, Pertusa, J., additional, Fernandez Colom, P. J., additional, Li, C., additional, Zhang, Y., additional, Cui, Y., additional, Zhao, H., additional, Liu, J., additional, Oliveira, J. B. A., additional, Petersen, C. G., additional, Mauri, A. L., additional, Massaro, F. C., additional, Silva, L. F. I., additional, Ricci, J., additional, Cavagna, M., additional, Pontes, A., additional, Vagnini, L. D., additional, Baruffi, R. L. R., additional, Franco Jr., J. G., additional, Felipe, V., additional, Vilela, M., additional, Tiveron, M., additional, Lombardi, C., additional, Viglierchio, M. I., additional, Marconi, G., additional, Rawe, V., additional, Wale, P. L., additional, Gardner, D. K., additional, Nakagawa, K., additional, Sugiyama, R., additional, Nishi, Y., additional, Kuribayashi, Y., additional, Jyuen, H., additional, Yamashiro, E., additional, Shirai, A., additional, Inoue, M., additional, Hovatta, O., additional, Tohonen, V., additional, Inzunza, J., additional, Parmegiani, L., additional, Cognigni, G. E., additional, Bernardi, S., additional, Ciampaglia, W., additional, Infante, F. E., additional, Tabarelli de Fatis, C., additional, Pocognoli, P., additional, Arnone, A., additional, Maccarini, A. M., additional, Troilo, E., additional, Filicori, M., additional, Radwan, P., additional, Polac, I., additional, Borowiecka, M., additional, Bijak, M., additional, and Radwan, M., additional

Published
2011
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28. Atlas muon detection using jet cells

Author

Dris, M, Filippas-Tassos, A, Gazis, E N, Bussmann, K, Chevalley, J L, Di Tore, G, Fabjan, Christian Wolfgang, Franz, A, Gaumann, E, Gayde, JC, Goret, B, Klempt, W, Lasseur, C, Raynaud, J, Rosso, E, Viehhauser, G, Barberio, E, Liguori, D, Schioppa, M, Susinno, G, Valdata-Nappi, M, Balla, A, Bilokon, H, Bonini, R, Chiarella, V, Curatolo, M, Corradi, G, Daniello, L, Esposito, B, Iacuessa, E, Iannotti, L, Passamonti, L, Russo, V, Santoni, M, Tentori, G, Teodoli, A, Troiani, M, Borisov, A, Bozhko, N, Gorjatchev, V, Kirsanov, M M, Kozhin, A, Salomatin, Yu I, Tumakov, V, Vovenko, A S, Bagnaia, P, Beker, H, Capradossi, G, Ciapetti, G, Nisati, A, Perciballi, M, Piscitelli, C, Pontecorvo, L, Kobayashi, T, Komamiya, S, and Mashimo, T

Subjects
Detectors and Experimental Techniques
Published
1993

30. Prototype tests of a “jet cell” drift chamber for large-area muon detection

Author

Bagnaia, P., primary, Barberio, E., additional, Beker, H., additional, Bilokon, H., additional, Bonini, R., additional, Borisov, A., additional, Bussmann, K., additional, Capradossi, G., additional, Chevalley, J.L., additional, Chiarella, V., additional, Ciapetti, G., additional, Curatolo, M., additional, Di Tore, G., additional, Dris, M., additional, Esposito, B., additional, Fabjan, C.W., additional, Filippas, T., additional, Franz, A., additional, Gaumann, E., additional, Gayde, J.Ch., additional, Gazis, E.N., additional, Goret, B., additional, Goryatchev, V., additional, Klempt, W., additional, Kobayashi, T., additional, Komamiya, S., additional, Kozhin, A., additional, Lasseur, C., additional, Liguori, D., additional, Mashimo, T., additional, Nisati, A., additional, Passamonti, L., additional, Perciballi, M., additional, Piscitelli, C., additional, Pontecorvo, L., additional, Raynaud, J., additional, Rosso, E., additional, Russo, V., additional, Schioppa, M., additional, Susinno, G., additional, Tentori, G., additional, Teodoli, A., additional, Nappi, M. Valdata, additional, and Viehhauser, G., additional

Published
1996
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34. A Light-Sensitive Yellow Ommochrome Pigment From the House Fly.

Author

BOLOGNESE, A., LIBERATORE, R., PISCITELLI, C., and SCHERILLO, G.

Abstract

A light-sensitive pigment (λ max at 430 and 340 nm), extracted in acidic methanol from the Musca domestica heads, showed, in the absorption curve, a plateau at 480-500 nm and a new maximum at 400 nm, after visible light irradiation. The light-sensitive house fly pigment showed spectroscopic and chemical properties of the ommochrome pigments (Butenandt and Schäfer: Recent Progress in the Chemistry of Natural and Synthetic, Colouring Matters and Related Fields, Academic Press, New York, pp 13-33, 1962; Bolognese and Scherillo: Experientia 30:225-226, 1974). The treatment of the extracted pigment with a methanol-HClsat. mixture afforded some coloured compounds; two main products were identified by comparison of their chromatographic and spectral properties with authentic samples of 1-oxo-2 H-3-carbomethoxy-5-methoxy-11-(fumaroylmethylester)-pyrido [3,2-a] phenoxazine (compound 7) and 1-oxo-2 H-3-carbomethoxy-5-methoxy-9-chlorine-11-(fumaroyl-methylester)-pyrido [3,2-a] phenoxazine (compound 8), obtained from the oxidation mixture of 3-hydroxykynurenine methylester (compound 9). [ABSTRACT FROM AUTHOR]

Published
1988
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35. Processing of a polycistronic mRNA requires a 5' cis element and active translation.

Author

Alifano, P., Piscitelli, C., Blasi, V., Rivellini, F., Nappo, A. G., Bruni, C. B., and Carlomagno, M. S.

Subjects
MESSENGER RNA, OPERONS, GENETIC regulation, SALMONELLA typhimurium, MICROBIAL mutation
Abstract

We have characterized a major processed species of mRNA in the his operon of Salmonella typhimurium. In vivo and in vitro analyses of the his transcripts from wild-type and mutant strains using $1 nuclease protection assays, measurements of RNA stability, deletion mapping, gel retardation, and in vitro translation assays demonstrate that the distal portion of the polycistronic his mRNA is processed, resulting in increased stability. The processing event requires an upstream cis-acting element and translation of the cistron immediately downstream of the 5' end of the processed species. The cistrons contained in this segment are also independently transcribed from an internal promoter which is maximally active in the absence of readthrough transcription from the primary promoter. [ABSTRACT FROM AUTHOR]

Published
1992
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39. Pistacia lentiscus L. fruits showed promising antimutagenic and antigenotoxic activity using both in-vitro and in-vivo test systems

Author

Ghania Bouguellid, Nadjet Debbache-Benaida, Dina Atmani-Kilani, Chiara Russo, Margherita Lavorgna, Concetta Piscitelli, Karima Ayouni, Meriem Berboucha-Rahmani, Marina Isidori, Djebbar Atmani, Bouguellid, G., Debbache-Benaida, N., Atmani-Kilani, D., Russo, C., Lavorgna, M., Piscitelli, C., Ayouni, K., Berboucha-Rahmani, M., Isidori, M., and Atmani, D.

Subjects
Umu test, Animal, Health, Toxicology and Mutagenesis, Toxicology, Plant Extract, Ames test, Pistacia lentiscusL, Antimutagenic Agent, micronucleus test, Mice, Fruit, Pistacia, Mutagen, antimutagenicity, Cyclophosphamide, Human
Abstract

Pistacia lentiscus L. is one of the most popular medicinal plants attributed to its beneficial properties on human health. However, few toxicogenetic studies have been carried out. Therefore, the aim of this study was to examine the potential genotoxic/antigenotoxic and mutagenic/antimutagenic properties of oil, ethyl acetate and ethanolic extracts of P. lentiscus L. fruits using in vitro the Ames and Umu assays, as well as in vivo micronucleus (MN) test. Extracts did not exert any significant mutagenic/genotoxic effects but provided protection against standard mutagenic and genotoxic agents including 2 nitrofluorene (2-NF) at 2.5 and 5µg/ml; sodium azide at 5 and 10µg/ml; 3-methylcholanthrene (3-MC) at 25 and 50μg/ml; cyclophosphamide (CP) at 50 and 100μg/ml; 4-nitroquinoline 1-oxide (4-NQO) at 0.05µg/ml and 2-amino-anthracene (AA) at 0.2µg/ml. Further, cytotoxicity and selectivity were examined on human hepatocarcinoma (HepG2), and MCF-7 breast cancer cell lines as well as a human normal-like fibroblast cell line (TelCOFS02MA) using MTT assay. Among all extracts, PF1 (ethanolic) showed the most significant selectivity index (SI) (HepG2:11.98; MCF7:4.83), which led to further investigations using an animal model. Oral administration of PF1 (125–1000 mg/kg b.w.) significantly decreased the number of micronucleated cells in CP -initiated (50 mg/kg b.w.) mice, while the number of micronucleated reticulocytes (MNRET), micronucleated polychromatic erythrocytes (MNPCE) or mitotic index (MI) were not markedly affected. Further, PF1 significantly enhanced catalase (CAT) and superoxide dismutase (SOD) activities in the livers and kidneys of these animals. The obtained results indicated the beneficial properties of P. lentiscus L. fruits for use in therapy against harmful effects of genotoxic and mutagenic agents. However, while promising it should be noted that the obtained results are preliminary and need to be confirmed prior to therapeutic use.

Published
2022
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40. Ribonuclease E provides substrates for Ribonuclease P-dependent processing of a polycistronic mRNA

Author

Pietro Alifano, Carmelo B. Bruni, C Piscitelli, F. Rivellini, Cecília M. Arraiano, M S Carlomagno, Alifano, P, R. I. V. E. L. L. I. N. I., . F., Piscitelli, C., Arraiano, C. M., Bruni, C. B., Carlomagno, MARIA STELLA, Rivellini, F, Piscitelli, C, Arraiano, Cm, Bruni, CARMELO BRUNO, Carlomagno, M. S., Alifano, Pietro, and Rivellini, F.

Subjects
Salmonella typhimurium, Genotype, Transcription, Genetic, RNase P, Ribonuclease E, Molecular Sequence Data, Restriction Mapping, Gene Expression, Ribonuclease P, Bacterial Proteins, Multienzyme Complexes, Endoribonucleases, Operon, Escherichia coli, Genetics, Histidine, RNA, Catalytic, Amino Acid Sequence, RNA, Messenger, Ribonuclease III, Ribonuclease, RNA Processing, Post-Transcriptional, Transaminases, Base Sequence, biology, Nuclear RNase P, Escherichia coli Proteins, Ribozyme, RNA, Molecular biology, Genes, Bacterial, Protein Biosynthesis, Transfer RNA, biology.protein, Nucleic Acid Conformation, Ribosomes, Developmental Biology
Abstract

The polycistronic mRNA of the histidine operon is subject to a processing event that generates a rather stable transcript encompassing the five distal cistrons. The molecular mechanisms by which such a transcript is produced were investigated in Escherichia coli strains carrying mutations in several genes for exo- and endonucleases. The experimental approach made use of S1 nuclease protection assays on in vivo synthesized transcripts, site-directed mutagenesis and construction of chimeric plasmids, dissection of the processing reaction by RNA mobility retardation experiments, and in vitro RNA degradation assays with cellular extracts. We have found that processing requires (1) a functional endonuclease E; (2) target site(s) for this activity in the RNA region upstream of the 5' end of the processed transcript that can be substituted by another well-characterized rne-dependent cleavage site; (3) efficient translation initiation of the first cistron immediately downstream of the 5' end; and (4) a functional endonuclease P that seems to act on the processing products generated by ribonuclease E. This is the first evidence that ribonuclease P, an essential ribozyme required for the biosynthesis of tRNA, may also be involved in the segmental stabilization of a mRNA.

Published
1994

41. Lymphocytes exposed to vegetables grown in waters contaminated by anticancer drugs: metabolome alterations and genotoxic risks for human health

Author

Brigida D’Abrosca, Antonio Fiorentino, Chiara Russo, Monica Scognamiglio, Margherita Lavorgna, Concetta Piscitelli, Vittoria Graziani, Marina Isidori, Russo, C., Graziani, V., Lavorgna, M., D'Abrosca, B., Piscitelli, C., Fiorentino, A., Scognamiglio, M., and Isidori, M.

Subjects
Adult, Male, Risk, 0301 basic medicine, 5-Fluorouracil, Health, Toxicology and Mutagenesis, Raphanus, Antineoplastic Agents, Lactuca, 010501 environmental sciences, Biology, medicine.disease_cause, Edible plant, 01 natural sciences, Human lymphocyte, Young Adult, 03 medical and health sciences, Metabolomics, Vegetables, Brassica rapa, Genetics, medicine, Metabolome, Humans, Lymphocytes, Food science, 0105 earth and related environmental sciences, food and beverages, biology.organism_classification, 6. Clean water, Comet assay, Metabolic pathway, 030104 developmental biology, Female, Comet Assay, Fluorouracil, Cisplatin, Genotoxicity, Water Pollutants, Chemical, DNA Damage, Mutagens
Abstract

Wastewater irrigation of crops may be effective to avoid depletion (about 70%) of freshwater resources. However, the use of reclaimed waters containing persistent microcontaminants such as antineoplastic drugs is of high environmental concern. These active compounds may affect human health with potentially severe adverse effects. To better understand the impact on human health following irrigation of crops with reused contaminated waters, we exposed four edible plants, Brassica rapa, Lactuca sativa, Raphanus sativus, and Triticum durum, to two commonly used antitumoral drugs: 5-fluorouracil (5-FU), and Cisplatin (CDDP), using metabolomics as a potential functional genomics tool to combine with genotoxicity experiments. The metabolome of the treated and untreated plants was analysed to detect biochemical alterations associated to the exposure, and the potential genotoxic damage related to human exposure to the treated plants was evaluated using the comet assay in human lymphocytes, which are characterized by high sensitivity to genotoxic substances. The edible species were able to assimilate 5-FU and CDDP during the treatment, affecting the biochemical pathways of these plants with subsequent metabolome modifications. These metabolic alterations differed according to the specific species used for the test. Furthermore, all vegetables treated with two concentrations of the selected drugs (10 and 100 μg/L) caused significant (p < 0.0001) genotoxic damage in the cells of the immune system at a higher level than in the lymphocytes directly exposed to single antineoplastic drugs.

Published
2019
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42. Capsaicin in Hot Chili Peppers: In Vitro Evaluation of Its Antiradical, Antiproliferative and Apoptotic Activities

Author

Roberta Nugnes, Elena Orlo, Concetta Piscitelli, Marina Isidori, Margherita Lavorgna, Chiara Russo, Lavorgna, M., Orlo, E., Nugnes, R., Piscitelli, C., Russo, C., and Isidori, M.

Subjects
Capsicum annuum, 0301 basic medicine, DPPH, Cytotoxicity, Apoptosis, Pharmacology, Cell Line, 03 medical and health sciences, chemistry.chemical_compound, 0404 agricultural biotechnology, Picrates, Tumor cell, Cell Line, Tumor, Humans, MTT assay, Benzothiazoles, Cell Proliferation, Normal-like cell, 030109 nutrition & dietetics, ABTS, Chemistry, Biphenyl Compounds, Free Radical Scavenger, Apoptosi, Free Radical Scavengers, 04 agricultural and veterinary sciences, Benzothiazole, Fibroblasts, 040401 food science, Chemistry (miscellaneous), Capsaicin, Cell culture, Fruit, Cancer cell, Biphenyl Compound, Antiradical activity, Fibroblast, Picrate, Selectivity index, Medicine, Traditional, Sulfonic Acids, Capsicum, Human, Food Science
Abstract

Capsaicin is a spicy capsaicinoid, produced as secondary metabolite by Capsicum fruits. This alkaloid has been used for years in folk medicine for its analgesic and antinflammatory properties although most data is referred to the raw fruit. In this study, the antiradical activity of the pure capsaicin has been studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays as well as its antiproliferative activity, using MTT assay, against two human tumour cell lines, the colorectal Caco-2 and the oesophageal OE19 cells. Furthermore, the antiproliferative activity observed on tumoral cells was compared with that of the human normal-like fibroblast cell line TelCOFS02MA. In addition, the apoptotic activity was evaluated using TUNEL assay. A higher radical scavenging activity was observed against ABTS radical cation than DPPH. Capsaicin showed also a higher cytotoxicity against cancer cells than normal-like cells with Selectivity index values greater than 2 at 72h. Capsaicin induced apoptosis especially in OE19 cell line.

Published
2019
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43. A cytosine- over guanosine-rich sequence in RNA activates rho-dependent transcription termination

Author

Pietro Alifano, Carmelo B. Bruni, C Piscitelli, F. Rivellini, Valeria Blasi, M S Carlomagno, Rivellini, F, Alifano, Pietro, Piscitelli, C, Blasi, V, Bruni, Cb, Carlomagno, Ms, Alifano, P, Bruni, CARMELO BRUNO, Carlomagno, M. S., Rivellini, F., Piscitelli, C., Blasi, V., Bruni, C. B. M. S., and Carlomagno, MARIA STELLA

Subjects
Transcription, Genetic, Termination factor, Genetic Vectors, Molecular Sequence Data, Guanosine, Biology, Regulatory Sequences, Nucleic Acid, Microbiology, chemistry.chemical_compound, Cytosine, Transcription (biology), Consensus Sequence, Operon, Consensus sequence, Escherichia coli, RNA Precursors, Histidine, Molecular Biology, Terminator Regions, Genetic, Base Composition, Base Sequence, RNA, Rho factor, Molecular biology, Rho Factor, chemistry, biology.protein, DNA
Abstract

We have constructed an expression vector carrying the Escherichia coli his operon control region to study the ability of defined segments of DNA to cause rho factor-mediated transcription termination both in vivo and in vitro. We have previously identified a consensus motif consisting of a region of high cytosine over guanosine content common to several cryptic intracistronic transcription termination elements unmasked by polar mutations. We show that a DNA fragment possessing features similar to the ones previously identified is capable of causing rho-mediated mediated release of transcripts in vivo and in vitro. The efficiency of termination depends on the length and efficiency of termination depends on the length and relative cytosine over guanosine ratio of the element.

Published
1991

44. A New Approach for Improving the Antibacterial and Tumor Cytotoxic Activities of Pipemidic Acid by Including It in Trimethyl-β-cyclodextrin

Author

Rosa Iacovino, Chiara Russo, Concetta Piscitelli, Cristina Di Donato, Margherita Lavorgna, Marina Isidori, Lavorgna, M, Iacovino, R, Russo, C, Di Donato, C, Piscitelli, C, and Isidori, M

Subjects
02 engineering and technology, medicine.disease_cause, 01 natural sciences, lcsh:Chemistry, X-Ray Diffraction, Spectroscopy, Fourier Transform Infrared, Solubility, Cytotoxicity, lcsh:QH301-705.5, trimethyl-β-cyclodextrin (TRIMEB), Spectroscopy, Antibacterial agent, chemistry.chemical_classification, Molecular Structure, Cyclodextrin, beta-Cyclodextrins, General Medicine, 021001 nanoscience & nanotechnology, Quinolone, Anti-Bacterial Agents, Computer Science Applications, 0210 nano-technology, Antibacterial activity, inclusion complex, medicine.drug, Cell Survival, medicine.drug_class, Antineoplastic Agents, Microbial Sensitivity Tests, microbial activity, Article, Catalysis, Inorganic Chemistry, Cell Line, Tumor, pipemidic acid, medicine, Humans, Physical and Theoretical Chemistry, Molecular Biology, Escherichia coli, antitumoral activity, Bacteria, Dose-Response Relationship, Drug, 010405 organic chemistry, Organic Chemistry, Pipemidic acid, 0104 chemical sciences, lcsh:Biology (General), lcsh:QD1-999, chemistry, Nuclear chemistry
Abstract

Pipemidic acid (HPPA) is a quinolone antibacterial agent used mostly to treat gram-negative infections of the urinary tract, but its therapeutic use is limited because of its low solubility. Thus, to improve drug solubility, natural cyclodextrins (CDs) are used for their ability of including guest molecules within their cavities. The aim of this work was to evaluate the antibacterial activity and the preliminary anticancer activity of HPPA included into Heptakis (2,3,6-tri-O-methyl)-β-cyclodextrin (TRIMEB) as a possible approach for a new innovative formulation. The inclusion complex of HPPA with TRIMEB was prepared in solid state by the kneading method and confirmed by FT-IR and powered X-ray diffraction. The association in aqueous solutions of pipemidic acid with TRIMEB was investigated by UV-Vis spectroscopy. Job’s plots have been drawn by UV-visible spectroscopy to confirm the 1:1 stoichiometry of the host–guest assembly. The antibacterial activity of HPPA, TRIMEB and of their complex was tested on Escherichia coli, Pseudomonas aeruginosa, and Staphilococcus aureus. The complex was able to increase 47.36% of the median antibacterial activity of the free HPPA against E. coli (IC50 = 249 µM vs. 473 µM). Furthermore, these samples were tested on HepG-2 and MCF-7. After 72 h, the median tumoral cytotoxicity exerted by the complex was increased by 78.08% and 94.27% for HepG-2 and MCF-7 respectively, showing a stronger bioactivity of the complex than the single HAPPA.

Published
2019
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45. Pre-Visioni per Milano. Racconti brevi

Author

PISCITELLI, Daniela, D. Piscitelli, C. Masciadri, and Piscitelli, Daniela

Subjects
Il disegno della città, Graphic Design, paesaggi e identità, brandingscape, Confini, racconti brevi, manifesto
Published
2015

46. Una presenza indispensabile

Author

Anceschi, G., Baroni, D., Bassi, A., Bosoni, G., Carmagnola, F., Pansera, A., Pasca, V., Riccini, R., Vitta, M., Bigatti, G., Bulegato, F., Dalla Mura, M., Dardi, D., Peruccio, P., PISCITELLI, Daniela, Vinti, C. ., G. Anceschi, D. Baroni, A. Bassi, G. Bosoni, F. Carmagnola, A. Pansera, V. Pasca, R. Riccini, M, Vitta, G. Bigatti, F. Bulegato, M. Dalla Mura, D. Dardi, P. Peruccio, D. Piscitelli, C. Vinti., Anceschi, G., Baroni, D., Bassi, A., Bosoni, G., Carmagnola, F., Pansera, A., Pasca, V., Riccini, R., Vitta, M., Bigatti, G., Bulegato, F., Dalla Mura, M., Dardi, D., Peruccio, P., Piscitelli, Daniela, and Vinti, C. .

Published
2013

47. Processing of a polycistronic mRNA requires a 5′ciselement and active translation

Author

Carmelo B. Bruni, Anna Giulia Nappo, C Piscitelli, Valeria Blasi, F. Rivellini, Pietro Alifano, M S Carlomagno, Alifano, Pietro, Piscitelli, C, Blasi, V, Rivellini, F, Nappo, Ag, Bruni, Cb, Carlomagno, Ms, Alifano, P, Bruni, CARMELO BRUNO, and Carlomagno, M. S.

Subjects
DNA, Bacterial, Salmonella typhimurium, RNA Stability, Transcription, Genetic, Operon, viruses, Molecular Sequence Data, In Vitro Techniques, Biology, Microbiology, Cistron, Transcription (biology), Gene expression, Escherichia coli, Histidine, Deletion mapping, RNA, Messenger, RNA Processing, Post-Transcriptional, Promoter Regions, Genetic, Molecular Biology, Messenger RNA, Base Sequence, Gene Expression Regulation, Bacterial, Molecular biology, RNA, Bacterial, Genes, Bacterial, Regulatory sequence, Protein Biosynthesis, Trans-Activators, Plasmids
Abstract

Summary We have characterized a major processed species of mRNA in the his operon of Salmonella typhimurium. In vivo and in vitro analyses of the his transcripts from wild-type and mutant strains using S1 nuclease protection assays, measurements of RNA stability, deletion mapping, gel retardation, and in vitro translation assays demonstrate that the distal portion of the polycistronic his mRNA is processed, resulting in increased stability. The processing event requires an upstream cis-acting element and translation of the cistron immediately downstream of the 5′ end of the processed species. The cistrons contained in this segment are also independently transcribed from an internal promoter which is maximally active in the absence of readthrough transcription from the primary promoter.

Published
1992
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48. Cytotoxic evaluation and chemical investigation of tomatoes from plants (Solanum lycopersicum L.) grown in uncontaminated and experimentally contaminated soils.

Author

Russo C, Barone D, Lavorgna M, Piscitelli C, Macaluso M, Pacifico S, Piccolella S, Giordano A, and Isidori M

Subjects
Environmental Pollution, Soil chemistry, Colorectal Neoplasms, Solanum lycopersicum metabolism, Metals, Heavy analysis, Soil Pollutants metabolism
Abstract

The aim of this study was to evaluate the cytotoxic activity and the chemical composition of the tomato extracts coming from, Pomodoro Giallo and San Marzano Cirio 3, and then to evaluate the potential changes when plants were grown in soils contaminated by cadmium, chromium and lead. Extracts were investigated by UHPLC-HRMS and UV-Vis. Cell viability (CellTiter-Glo Luminescent assay), enzyme aldehyde dehydrogenase activity (ALDEFLOUR Assay), cell cycle progression (Accuri C6 Flow Cytometer), apoptosis and necrosis (Annexin V-FITC assay) were evaluated on two gastric cancer (AGS and NCI-N87) and two colorectal cancer (HT-29 and HCT 116) cell lines. Different content of polyphenol and carotenoid constituents was observed. Extracts from uncontaminated soil induced cytotoxic activity towards all selected cancer cells, while extracts coming from contaminated soils showed the aberrant phenotype increased in colorectal cancer cells. Chloroform extracts exerted the highest cytotoxic activity. AGS and HT-29 were the most sensitive to cell cycle arrest and to apoptosis. No necrotic effect was observed in HCT 116. The contrasting effects on cancer cells were observed based on tomato variety, the extract polarity, heavy metal identity, and tested cell line. The investigation of potential adverse health effects due to Cd in the fruits should be explored., (© 2022. The Author(s).)

Published
2022
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49. Microalgal Co-Cultivation Prospecting to Modulate Vitamin and Bioactive Compounds Production.

Author

Pistelli L, Mondo AD, Smerilli A, Corato F, Piscitelli C, Pellone P, Carbone DA, Sansone C, and Brunet C

Abstract

Microalgal biotechnology is gaining importance. However, key issues in the pipeline from species selection towards large biomass production still require improvements to maximize the yield and lower the microalgal production costs. This study explores a co-cultivation strategy to improve the bioactive compounds richness of the harvested microalgal biomass. Based on their biotechnological potential, two diatoms ( Skeletonema marinoi , Cyclotella cryptica ) and one eustigmatophyte ( Nannochloropsis oceanica ) were grown alone or in combination. Concentrations of ten vitamins (A, B 1 , B 2 , B 6 , B 12 , C, D 2 , D 3 , E and H), carotenoids and polyphenols, together with total flavonoids, sterols, lipids, proteins and carbohydrates, were compared. Moreover, antioxidant capacity and chemopreventive potential in terms inhibiting four human tumor-derived and normal cell lines proliferation were evaluated. Co-cultivation can engender biomass with emergent properties regarding bioactivity or bioactive chemical profile, depending on the combined species. The high vitamin content of C. c ryptica or N. oceanica further enhanced (until 10% more) when co-cultivated, explaining the two-fold increase of the antioxidant capacity of the combined C. c ryptica and N. oceanica biomass. Differently, the chemopreventive activity was valuably enhanced when coupling the two diatoms C. cryptica and S. marinoi . The results obtained in this pilot study promote microalgal co-cultivation as a valuable strategy aiming to boost their application in eco-sustainable biotechnology.

Published
2021
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50. Natural Compounds of Marine Origin as Inducers of Immunogenic Cell Death (ICD): Potential Role for Cancer Interception and Therapy.

Author

Sansone C, Bruno A, Piscitelli C, Baci D, Fontana A, Brunet C, Noonan DM, and Albini A

Subjects
Antineoplastic Agents pharmacology, Clinical Trials as Topic, Humans, Aquatic Organisms chemistry, Biological Products pharmacology, Immunogenic Cell Death drug effects, Neoplasms pathology, Neoplasms therapy
Abstract

Regulated cell death (RCD) has always been considered a tolerogenic event. Immunogenic cell death (ICD) occurs as a consequence of tumour cell death accompanied by the release of damage-associated molecular patterns (DAMPs), triggering an immune response. ICD plays a major role in stimulating the function of the immune system in cancer during chemotherapy and radiotherapy. ICD can therefore represent one of the routes to boost anticancer immune responses. According to the recommendations of the Nomenclature Committee on Cell Death (2018), apoptosis (type I cell death) and necrosis (type II cell death) represent are not the only types of RCD, which also includes necroptosis, pyroptosis, ferroptosis and others. Specific downstream signalling molecules and death-inducing stimuli can regulate distinct forms of ICD, which develop and promote the immune cell response. Dying cells deliver different potential immunogenic signals, such as DAMPs, which are able to stimulate the immune system. The acute exposure of DAMPs can prime antitumour immunity by inducing activation of antigen-presenting cells (APC), such as dendritic cells (DC), leading to the downstream response by cytotoxic T cells and natural killer cells (NK). As ICD represents an important target to direct and develop new pharmacological interventions, the identification of bioactive natural products, which are endowed with low side effects, higher tolerability and preferentially inducing immunogenic programmed cell death, represents a priority in biomedical research. The ability of ICD to drive the immune response depends on two major factors, neither of which is intrinsic to cell death: 'Antigenicity and adjuvanticity'. Indeed, the use of natural ICD-triggering molecules, alone or in combination with different (immuno)therapies, can result in higher efficacy and tolerability. Here, we focused on natural (marine) compounds, particularly on marine microalgae derived molecules such as exopolysaccharides, sulphated polysaccharides, glycopeptides, glycolipids, phospholipids, that are endowed with ICD-inducing properties and sulfavants. Here, we discuss novel and repurposed small-molecule ICD triggers, as well as their ability to target important molecular pathways including the IL-6, TNF-α and interferons (IFNs), leading to immune stimulation, which could be used alone or in combinatorial immunotherapeutic strategies in cancer prevention and therapies.

Published
2021
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