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9 results on '"Pisarchyk, Liudmila"'

3. Long-term dynamics of somatosensory activity in a stroke model of distal middle cerebral artery oclussion

Author

Barios Heredero, Juan Antonio, Pisarchyk, Liudmila, Fernández García, Laura, Barrio, Luis C., Ramos Gómez, Milagros, Martínez Murillo, Ricardo, González Nieto, Daniel, Barios Heredero, Juan Antonio, Pisarchyk, Liudmila, Fernández García, Laura, Barrio, Luis C., Ramos Gómez, Milagros, Martínez Murillo, Ricardo, and González Nieto, Daniel

Abstract

A constant challenge in experimental stroke is the use of appropriate tests to identify signs of recovery and adverse effects linked to a particular therapy. In this study, we used a long-term longitudinal approach to examine the functional brain changes associated with cortical infarction in a mouse model induced by permanent ligation of the middle cerebral artery (MCA). Sensorimotor function and somatosensory cortical activity were evaluated with fault-foot and forelimb asymmetry tests in combination with somatosensory evoked potentials. The stroke mice exhibited both long-term deficits in the functional tests and impaired responses in the infarcted and intact hemispheres after contralateral and ipsilateral forepaw stimulation. In the infarcted hemisphere, reductions in the amplitudes of evoked responses were detected after contralateral and ipsilateral stimulation. In the intact hemisphere, and similar to cortical stroke patients, a gradual hyperexcitability was observed after contralateral stimulation but no parallel evidence of a response was detected after ipsilateral stimulation. Our results suggest the existence of profound and persistent changes in the somatosensory cortex in this specific mouse cortical stroke model. The study of evoked potentials constitutes a feasible and excellent tool for evaluating the fitness of the somatosensory cortex in relation to functional recovery after preclinical therapeutic intervention.

Published
2016

8. Dietary restriction in obese children and its relation with eating behavior, fibroblast growth factor 21 and leptin: a prospective clinical intervention study.

Author

del Rocío Ibarra-Reynoso, Lorena, Pisarchyk, Liudmila, Pérez-Luque, Elva Leticia, Garay-Sevilla, Ma. Eugenia, and Malacara, Juan Manuel

Subjects
*ANALYSIS of variance, *CHILD nutrition, *DIET in disease, *DIET therapy, *ENZYME-linked immunosorbent assay, *EXPERIMENTAL design, *FIBROBLASTS, *FOOD habits, *GROWTH factors, *LONGITUDINAL method, *LOW-carbohydrate diet, *LOW-fat diet, *CHILDHOOD obesity, *QUESTIONNAIRES, *RADIOIMMUNOASSAY, *RESEARCH funding, *T-test (Statistics), *LEPTIN, *MULTIPLE regression analysis, *TREATMENT effectiveness, *REPEATED measures design, *DESCRIPTIVE statistics
Abstract

Background: Obesity is significant problem involving eating behavior and peripheral metabolic conditions. The effect of carbohydrate and fat restriction on appetite regulation, fibroblast growth factor 21 (FGF21) and leptin in children has not been defined. Our objective was to compare the effect of both diets. 2 Methods: One hundred and twenty children with body mass index (BMI) higher than the equivalent of 30 kg/m for an adult, as corrected for gender and age were randomly assigned to (n = 60) a low-carbohydrate (L-CHO) diet or (n = 60) a low-fat (L-F) diet for 2 months. Fifty-three (88.3 %) subjects on the low-carbohydrate-diet and 45 (75 %) on the low-fat diet completed the study. Anthropometric measures, leptin and FGF21 levels were measured before and after the intervention. Comparison of the data for both of the diet groups was carried out using the t-test for independent variables. Intragroup comparisons before and after of each of the dietary treatments were performed using ANOVA for repeated measures. Factors associated with FGF21, leptin levels and satiety, were analyzed by multiple regression. Results: After both of the diets, weight, leptin, food responsiveness, and enjoyment of food significantly decreased and high density lipoprotein cholesterol (HDL) increased, but FGF21 decreased. Before and after both of the interventions FGF21 was associated with triglycerides. Before the diet, satiety was associated with lower screen time (p < 0.04) and insulin levels (p < 0.05). Conclusions: Both dietary restrictions improved the metabolic and hormonal parameters of obese children. FGF21 is an indicator of a beneficial metabolic response in younger children. After 2 months an adaptation of the eating behavior to food restriction was observed. [ABSTRACT FROM AUTHOR]

Published
2015
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