70 results on '"Piri, H."'
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2. Investigation of the Effect of Biochar on the Physical and Chemical Properties of Soil under Quinoa Cultivation under Water and Salinity Stress Conditions.
- Author
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Tourajzadeh, O., Piri, H., Naserin, A., and Chari, M. M.
- Abstract
Introduction Appropriate and practical use of agricultural waste reduce the pressure on the environment. Recently, there has been significant promotion of biochar utilization in agricultural lands. It serves as a valuable source of organic material for enhancing plant growth and as an effective soil amendment to improve soil properties. Due to its unique chemical and physical properties, biochar can be used as a soil conditioner and has many benefits for optimal agricultural and environmental management. Studies have shown that biochar is a useful amendment for improving the physical and chemical properties of soil and effective in maintaining organic matter and soil moisture. Materials and Methods This research was conducted with the aim of investigating the effects of biochar on the physical and chemical properties of soil under conditions of water stress and irrigation using saline water. The experiment was carried out in a factorial based on a completely randomized design with three replications in greenhouse conditions. The treatments include three irrigation water treatments (60, 80, and 100 percent water requirement of the plant, respectively, I1, I2, and I3), three treatments of biochar prepared from northern forest trees at a temperature of 300 degrees Celsius (0, 2, and 4 percent by weight of potting soil, respectively, B1, B2, and B3) and three water quality treatments (with electrical conductivity 1, 4 and 7 dS/m, respectively, S1, S2 and S3). The pots were weighed every other day and at each level of biochar and salinity, the water deficit up to the agricultural moisture level was calculated based on the changes in the pot's weight. After harvesting (in the first half of April 2022), in order to investigate the effect of biochar on the amount of soil nutrients and some physical and chemical parameters of the soil under the conditions of water stress and irrigation water salinity, sampling was done from the soil of each pot. The samples were taken to the laboratory and parameters of apparent and actual specific gravity, acidity and salinity of the soil, percentage of nitrogen, phosphorus and potassium absorbable of the soil were measured in the laboratory. Referring to the yield to irrigation water ratio, water productivity is obtained by the following relation (Payero et al., 2009): WP=Y/IR, where, WP represents water productivity (kg/m³), Y denotes the yield (kg/ha), and IR shows the amount of irrigation water (m³/ha). Analysis of variance for the results obtained from different treatments was conducted using SAS software (SAS 9.1, SAS Institute, Cary, NC, USA). The mean values of the main factors and interactive effects were compared using the Duncan method at the 1% and 5% levels of significance. Results and Discussion The results showed that the amount of biochar, irrigation water and water salinity and their mutual effects had a significant effect on the measured parameters at the probability level of one and five percent. Adding 2 and 4 mass percent biochar to the soil increased the amount of phosphorus (35 and 60%, respectively), potassium (57% and 61%), nitrogen (83% and 91%), pH (13% and 13%) and electrical conductivity (EC) (13% and 57%) of the soil. By adding 2% and 4% of biochar to the soil, the actual specific gravity of the soil decreased by 13% and 21%, respectively, and the apparent specific gravity decreased by 11% and 22%, respectively. The actual and apparent specific gravity of the soil decreased by adding biochar to the soil. Decreasing the depth of irrigation water and increasing water salinity increased the amount of phosphorus, potassium, nitrogen, pH and EC of the soil. The amount of irrigation water had no significant effect on the apparent and actual specific gravity, however, the salinity of the irrigation water caused a significant increase in the apparent and actual specific gravity of the soil. Although the addition of biochar to the soil increased the nutrients required by plants in the soil, high amounts of biochar in the soil should be used careful, because the addition of this organic matter to the soil at high levels increased soil EC significantly. Based on the findings derived from the research, the utilization of biochar is recommended as a viable approach for enhancing both the chemical quality and productivity of nutrient-poor and sandy soils. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Approximating fixed points of generalized α-nonexpansive mappings in Banach spaces by new faster iteration process
- Author
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Piri, H., Daraby, B., Rahrovi, S., and Ghasemi, M.
- Published
- 2019
- Full Text
- View/download PDF
4. Activity, Stability, and Structure of Native and Modified by Woodward Reagent K Mushroom Tyrosinase
- Author
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Emami, S., Piri, H., and Gheibi, N.
- Published
- 2018
- Full Text
- View/download PDF
5. The Effect of Combined Use of Vermicompost and Poultry Manure on the Growth and Yield of Cucumber Plants in Different Conditions of Deficit Irrigation.
- Author
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Behdarnejad, M., Piri, H., and Delbari, M.
- Subjects
VERMICOMPOSTING ,POULTRY manure ,DEFICIT irrigation ,COMPOSTING ,FRUIT weights & measures - Abstract
Introduction In sustainable farming systems, the use of organic fertilizers is of particular importance in increasing crop production and maintaining sustainable soil fertility. Nowadays, the consumption of organic foods is introduced to consumers as an alternative. The result of the application of chemical products is the crisis of environmental pollution, soil and water resources, and the health risk to human society. Nowadays, in order to reduce the effects of misuse of chemical inputs, chemical fertilizers can be replaced with organic biological fertilizers, including animal manure, compost, and green manure. In this regard, chicken manure has a positive effect on the physical, chemical, and biological characteristics of the soil, and due to its richness in uric acid, the nitrogen contained in it is used by the plant much faster than the nitrogen of other organic fertilizers. Vermicompost is considered a good source of soil fertility due to its organic materials. Organic matter in the soil improves the permeability and drainage of the soil and also prevents excessive dryness of the soil by maintaining sufficient moisture. Despite the fact that vermicompost can be used as a fertilizer in organic farming, high levels of this fertilizer may cause salinity effects in the plant, which affects the growth and development of the plant and even it can cause the death of cucumber as one of the crops sensitive to soil and water salinity. The cucumber (Cucumis sativus L.) is one of the important vegetables that can be produced in a greenhouse all year round. Fresh consumption of cucumber throughout the year has increased its production. The development of technology and the short growth period of this product has made it possible to grow it in most climate zones. Therefore, in this research, the effects of different levels of water deficit with the simultaneous application of vermicompost and chicken manure on cucumber plants in the Behbahan region have been investigated. Materials and Methods In this study, different levels of irrigation water, vermicompost, and poultry manure on ground cucumber were investigated. The experiment was performed in the form of split plots based on completely randomized design and the form of stacks. Treatments included three levels of poultry manure (2, 4 and 8 ton ha-1), three levels of vermicompost (3, 6 and 9 ton ha-1) and three levels of water stress (100, 75 and 50% of plant water requirement). Both vermicompost and poultry manure were applied to the soil before planting. Harvest was done every three days. Fruit weight, diameter and length, plant length, the protein of the dry matter of the fruit percentage, and leaf chlorophyll in each plot were carefully measured. Also, the yield and water productivity at the end of the season were calculated. Water productivity Referring to the yield to irrigation water ratio, is obtained by the following relation (Payero et al., 2009): WP=Y/IR (1) In this equation, WP represents water productivity (kg/m3), Y denotes the yield (kg/ha), and IR shows the amount of irrigation water (m3/ha). Statistical analysis The analysis of variance for the results obtained from different treatments was conducted using SAS software (SAS 9.1, SAS Institute, Cary, NC, USA). The mean values of the main factors and interactive effects were compared using the Duncan method at the 1% and 5% levels of significance. Results and Discussion The results showed that irrigation, poultry manure and vermicompost had a significant effect on the measured parameters at the level of one and five percent probability. Reduction of water consumption reduced yield and yield components, but in this regard, no significant difference was observed between 100% and 75% of water requirement. The highest yield was obtained in the treatment of 100% of plant water requirement and consumption of 4 ton ha-1 of poultry manure and 6 ton ha-1 of vermicompost, in this regard, no significant difference was observed with the treatment of 75% of water requirement. According to the results obtained from this study, it can be said that there is no significant difference in terms of yield between treatments of 75 and 100% of plant water requirement. Therefore, the amount of water given to the plant can be reduced to 75% of the plant water requirement, and with proper management, less water can be consumed without a significant reduction in crop yield. Examining the effects of irrigation water on the amount of the protein of the dry matter of the fruit showed that the highest amount of the protein of the dry matter of the fruit (56.31%) was obtained in the treatment of 75% of the water requirement and the protein of the dry matter of the fruit was less in other treatments. The interaction effect of vermicompost and poultry manure resulted in the highest percentage of cucumber protein at a treatment of 4 tons ha-1 of poultry manure and 6 tons ha-1 of vermicompost (58.42%). However, when the simultaneous use of 8 tons ha-1 of poultry manure and different levels of vermicompost was employed, the percentage of protein in the fruit's dry matter decreased. The combination of drought stress, poultry manure, and vermicompost, along with their interaction effects, significantly influenced the chlorophyll a and b values at both the 1% and 5% probability levels. As the depth of irrigation water decreased, the amounts of chlorophyll a and b also decreased. The treatment with 100% water requirement of the plant showed the highest amounts of chlorophyll a (0.63 mg/g fresh weight) and chlorophyll b (0.36 mg/g fresh weight). However, no significant difference was observed compared to the 75% treatment. Regarding the interactions between vermicompost and poultry manure, it was found that when using 6 tons ha-1 of vermicompost to reduce yield and its components, the use of poultry manure should be reduced to 4 tons ha-1. On the other hand, when higher levels of vermicompost (9 tons ha-1) are used, the application of poultry manure should be reduced to 2 tons ha-1. Result According to the results obtained from this research, it can be said that there is no significant difference in performance between the treatments of providing 75% and 100% of the water requirement of the plant, therefore, the amount of water given to the plant can be reduced to the amount of 75% of the water requirement of the plant. With proper management, less water can be consumed without significantly reducing the yield of the product. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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6. Th-1 cytokines gene polymorphism in human brucellosis
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Davoudi, S., Amirzargar, A. A., Hajiabdolbaghi, M., Rasoolinejad, M., Soodbakhsh, A., Jafari, S., Piri, H., Maleknejad, P., Bagherian, H., Madadi, N., and Nikbin, B.
- Published
- 2006
7. Study on biological status of the Gorgan Bay
- Author
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Mohamadkhani, Hassan, Hami tabari, A., Porsoufi, T., Shirangi, S., Mansouri, B., Taheri shahraeeni, Piri, H., and Porgholam, R.
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Salinity ,Physic-chemical parameters ,Trophy level ,Biological ,Productivity - Abstract
Present study carried out between September 2011 and October 2012 in 19 sampling sites in order to investigate the trophy level, productivity, and natural dominant living conditions in the Gorgan Bay. According to the provided maps from the shoreline, depth, and sediment, the total area and volume of Gorgan Bay is 466 square meters and 905.33 million cubic meters respectively. Also the physic-chemical parameters including: water temperature, EC, salinity, transparency, DO, BOD5, pH, Ammonia, Nitrate, Total hardness, Total alkalinity, and phosphate are determined and studies. 3 main phylum, 12 orders, 6 classes, and 12 families from bottom living organisms (macro-benthos) were identified in this region. Standard deviation in TSI was from minimum 37 in March up to maximum 65 in September and the annual average was 53. According to the average TSI in different months, for five months namely as March, April, June, August, and February the dominant situation was eutrophy. For the rest of months namely as May, September, October, November, December, and January the dominant situation was Meso-trophic in the Bay. Based on calculation, minimum and maximum productivity in the Bay were 92.26 and 700.66 Kg per hectare in October and July respectively. The annual average of productivity was 195.10 Kg per hectare. Iranian Fisheries Science Research Institute Published
- Published
- 2017
8. Approximating fixed points of generalized α-nonexpansive mappings in Banach spaces by new faster iteration process
- Author
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Piri, H., primary, Daraby, B., additional, Rahrovi, S., additional, and Ghasemi, M., additional
- Published
- 2018
- Full Text
- View/download PDF
9. Khan type fixed point theorems in a generalized metric space
- Author
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Piri, H., primary, Rahrovi, S., additional, and Kumam, P., additional
- Published
- 2016
- Full Text
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10. Mortality factors in artificially reproduced grey mullet (Mugil cephalus L.) fries
- Author
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Mirhashemi Rostami, S.A., Amini, K., Jorjani, M., Piri, H., Hami Tabari, A., Iri, Y., Shafeii, A., Saghali, M., and Poursoufey, T.
- Subjects
Golestan Province ,Mugil cephalus ,Larvae culture ,critical points ,fungi ,Artificial peopagation ,Aquaculture ,Mortality ,Iran ,Grey mullet - Abstract
Cultured nine year old breeders of Mugil cephalus L. were subjected to eight artificial propagation treatments from December to February, 2003. Some female breeders received two injections in an interval of 24 hours, and others received a gradual daily injection of 500 IU HCG per kilogram of body weight for five days and then subjected to the two injection protocol. Male breeders were given 5-10mg of 17-a MT in addition to HCG. The result of the process was the production of 117-2000,000 larvae in six series for each breeder. Water temperature and salinity during artificial propagation process were 20-23C and 32ppt, respectively. For larvae culture two trials were conducted. The final density of larvae was 20 individuals per liter. Water temperature and salinity in larvae culture tanks were 22-24C and 32-33ppt, respectively. The larvae were fed from their second day post-hatching with algae Nannochloropsis oculata with a density of 500000 cells/l, rotifer Brachionus plicatilis with a density of 20 individuals/ml and also Artemia nauplii with a density of 3-200 individuals/l. The particle size of dry feed used was 100, 300 and 500 microns. The daily water exchange in larval culture tanks at the first, second and third weeks of post- hatching were 10, 20-30 and 40-50 percent respectively. In the first round of larval culture trials which were carried out by means of circular tanks under water 80 cm deep; all larvae died 12 days post-hatching. In the second experiment, which was carried out by three black color fiberglass tanks with water 100 cm deep, the survival rate was 0.9 percent 50 days post-hatching. There are two vertical migrations during first two weeks of the grey mullet larvae culture. The first sinking takes place after the second day post-hatching and is probably related to the rapid absorption of the yolk sac and the resulting change in specific gravity. The second sinking happened 8-11 days post-hatching and is probably related to the full absorption of oil globule and the resulting increase in specific gravity. These migrations were found coinciding with high larval mortalities (critical points). It seems that the characteristics of larval culture tank such as shape, volume and depth, the nutritional circumstances of larvae and the aeration procedure are important and effective factors for the mullet to pass successfully from these critical periods.
- Published
- 2008
11. Identification and biological characteristics of Gobiidae in coastal area of the southern Caspian Sea (Guilan Province)
- Author
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Piri, H., Keyvan, A., Piri, M., and Yelghi, S.
- Subjects
Benthophilus stellatus ,Nematoda ,Hypaniula ,Length-weight relationships ,Length ,Fisheries ,Abra ,Iran ,Weight ,Chironomidae ,Benthophilus ctenolepidus ,Neogobius caspius ,Mysidae ,Benthophilus ,Cumacae ,Carididae ,Neogobius melanostomus ,Neogobius fluviatilis ,Caspian Sea ,Condition factor ,Gobiidae ,Neogobius kessleri ,Biology ,Gammaridae ,Brackish - Abstract
We selected five sampling sites to identify Goby species in the southern Caspian Sea (Guilan Province). Results showed that the Goby specimens in this area belong to the genus Neogobius and Benthophilus. Four species from the Neogobius were identified which were as follows: N. caspius, N. fluviatilis, N. melanostomus and N. kessleri. Two species from the genus Benthophilus were identified as B. stellatus and B. ctenolepidus. As for the biological characteristics of the N. caspius, we found that the average total length (TL) of N. caspius samples was 112.98±19.87mm and their average total weight (TW) was 16.39±6.21 grams. Age composition of the samples showed the fish were in age groups 1-3 years old with the two years old fish being dominant. Average absolute fecundity was 1031.91±598 eggs. Food items of the species based on the gut content were from Carididae, Mysidae, Cumacae, Hypaniula, hironomidae, Nematoda, Abra, Gammaridae, Mytilaster, Bivalves and fish. Evidence of carnivore behavior was observed in some specimens. The most frequent food items were Cumacae, Abra, and Gammaridae respectively. The Cumacae was mostly observed in the gut of 1 and 2 years old specimens while Abra was observed mostly in the gut content of 3 years old specimens. Condition factor and feeding rate were highest in the 1 year old class. Fish age increased, other parameters declined. However, again at the age of 3, the parameters showed a slight increase. Relative gut length also increased from 0.97 in the age class 0^+ to 1.38 in the age class 3^+.
- Published
- 2007
12. Stock assessment of the bony fishes of Golestan province
- Author
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Bandani, Gholamali, Piri, H., and Moradian, F.
- Subjects
Species ,Biometric ,Cyprinus carpio ,Fisheries ,Golestan province ,CPUE ,Bony fishes ,Caspian Sea ,Stock assessment ,Iran ,Common carp - Abstract
Total catches of bony fishes in the coastal of south Caspian Sea have been ended to 1554 ton in 1382. The catch of common carp Cyprinus carpio as a one of important commercial species has been lower than others species such as mahisephid, kolmeie and kafal. From commercial catch point of view, the catch of kolmeie is not very important, so that its catch was included only 2% of total bony fish catch in three provinces which are located in south of Caspian sea . In spite of lower common carp catch, in comparison with total bony fish catch in Golestan province more thanb70% catch was belong to Common carp. The higher catch kolmeie among three provinces was related to Guilan with 46% of total catches. Seasonal changes of common carp catch showed maximum and minimum catch were in April 83 and December 82 respectively. The most of (CPUE) was in April 83 and September, October 82. The trend of kolmeie catch showed low fluctuation. Three age groups were specified in kolmeie age composition high and low percent observed in 2 and 3 age groups respectively. Result of biometric information common carp showed most of catch was under standard fisheries catch.
- Published
- 2007
13. A SOLUTION OF THE NEW CAPUTO-FABRIZIO FRACTIONAL KDV EQUATION VIA STABILITY.
- Author
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AYDI, H., MARASI, H. R., PIRI, H., and TALEBI, A.
- Subjects
CAPUTO fractional derivatives ,FRACTIONAL differential equations ,KORTEWEG-de Vries equation ,STABILITY theory ,MATHEMATICAL analysis - Abstract
The new fractional derivative, which has no singular kernel, was recently introduced by Caputo and Fabrizio. In this paper, we consider the nonlinear KdV equation with the new fractional derivative and we study the existence of a solution by using H-stability of an iteration procedure. Numerical simulations are also reported to show H-stability of presented algorithm. [ABSTRACT FROM AUTHOR]
- Published
- 2017
14. FIXED POINT THEOREMS FOR GENERALIZED (ψ,φ)-WEAK CONTRACTIONS.
- Author
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PIRI, H. and RAHROVI, S.
- Subjects
FIXED point theory ,THEORY of distributions (Functional analysis) ,CONTRACTIONS (Topology) - Abstract
In this paper, we prove some fixed point theorems for generalized (ψ,φ)- weak contractive mappings in a metric space. Our result generalized and extend recent results of Singh et al.[16, Theorem 2.1], Dorić [7, Theorem 2.1], Rhoades [15, Theorem 1] and Dutta and Choudhary [9, Theorem 2.1]. Also, we provid an example to support the useability of our results. [ABSTRACT FROM AUTHOR]
- Published
- 2017
15. Algal mass Culture and Survey of Economic Aspects Emphasis on Green and Blue green Algae
- Author
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Fallahi Kapoorchali, Maryam, Salavatian, S.M., Piri, H., Ramezani, R., and Mohammadi, S.
- Subjects
Species ,Culture ,Bony fishes ,Mass ,Nostoc caraneum ,Fatty acid ,Oscillatoria affricanum ,Anabaena bergii ,Blue-green algae ,Scenedesmus obliquus ,bacteria ,Economic value ,Green algae ,Chlorella vulgaris - Abstract
The Isolation , Purify , semi mass culture and mass culture of algae was started in bony fishes research center of Guillan from 2001-2004.during this survey 39 species of algae stock remained that all of them were pirified. Some of economic valuation of algae were studied ,forexample: food for aquaculture , protein, lipid and toxin extraction and antibacterial property. The result showed that Rodomonas sp. Initiate the most of growth and survival for Daphnia magna because this species have a lot of unsaturation fatty acid (w3) , also the species of cyclotellasp. was fed better than other algae by silver carp in this survey . amount of toxin was a little in most of algae. The species of Anabaena spp1. , Anabaena flos-aquae have Antibacterial property but Anabaena bergii, Nostoc caraneum and Oscillatoria affricanum have antifungal property.the both Antibacterial and antifungal property observed in Anabaena variabilis , Anabaena spp2.and Anabaena spp3.amount of protein in green and blue-green algae wasrespectively 51±8 and 60±10 percent of dry weight . Total lipid amount was 12±0.4 , 8±0.3 and 2±0.8 percent of dry weight respectively in chlorella vulgaris, Scenedesmus obliquus , Anabaena flos- aquae. Iranian Fisheries Science Research Institute Published
- Published
- 2005
16. Stock assessment of bonyfishes in the Iranian water of the Caspian Sea ( 2001 2002 )
- Author
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Ghaninejad, Davood, Abdolmalaki, Sh., Saiyad Boorani, M., Poorgholami Moghaddam, A., Marakha, S., Bandani, Gh., Abbasi, K., Piri, H., Razavi Sayyad, B., and Parafkande Haghighi, F.
- Subjects
Species ,Bonyfishes ,Stock assessment ,Season ,Growth parameters - Abstract
For gathering of data on biometry and catch statfstics, 5, mobile working group in the regions of Anzali , Kiashahr , Noshahr , Babolsar and Torkaman . Were involved from the start to the end of fishing season. After gathering,data Were intered to the computer based on species. For data analysis different methods Were undertaken sach as Bertalanfy equation for growth parameter Beverton and Holt for total mortality rates, pauly's imperical model for natural mortality rate and cohort Analysis for biomas estimation. The total recorded Catch in 2001 and 2002 was 10529 and 9566 tonnes. Totall catch with taking into account of pauching . estinmated 16200 and 15200 tonnes .total catch of Katum in 2002 had 1200 tonnes decrease with comparison of last year. Biomass of kutum in IRanian coastal Water of the Caspian sea in 2002 estimated about 15000tonnes. The catch of golden mullet in 2001 and 2002 was estimated 4218 and 6183 tonnes.that about 29%-38% of the catch of this species was undersize .The biomass of golden mullet estimated about 16400 tonnes. The small nose mullet in 2002 comprise 9% of mullet catches. The catches of common carp in Anzali region was very low .but stocks of this species in Gorgan region is in better condition which.Most of catches of this species were fished illgally in the Gorgan bay and Gomishan coastal creek. The biomass of this species was estimated 1140 tonnes Most of catches of the roach.were fished illgally .Totall catches of this species in 2002 were estimated about 738 tonnes. The catch of pickperch in 2002 were estimated about 30 tonnes.Most of the chatch of pickperch was immature and undersize. Appooximattelly all of the catch is due to releasing of fingerling of this species by fishery company. Totall catch of bream in 2002 was estimated 38 tonnes that most of which was immature and undersize. Iranian Fisheries Science Research Institute Published
- Published
- 2005
17. Total catches of bony fishes in the coastal of south Caspian Sea have been ended to 1554 ton in 1382
- Author
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Bandani, Gh.A. and Piri, H.
- Subjects
Bony fish ,Cyprinus carpio ,CPUE ,Common carp - Abstract
The catch of common carp Cyprinus carpio as a one of important commercial species has been lower than others species such as mahisephid ,kolmeie and kafal. From commercial catch point of view, the catch of kolmeie is not very important, so that its catch was included only 2% of total bony fish catch in three provinces which are located in south of Caspian sea . In spite of lower common carp catch, in comparison with total bony fish catch in Golestan province more than 70% catch was belong to Common carp. The higher catch kolmeie among three provinces was related to Guilan with 46% of total catches. Seasonal changes of common carp catch showed maximum and minimum catch were in April 83 and December 82 respectively. The most of (CPUE) was in April 83 and September, October 82. The trend of kolmeie catch showed low fluctuation. Three age groups were specified in kolmeie age composition high and low percent observed in 2 and 3 age groups respectively. Result of biometric information common carp showed most of catch was under standard fisheries catch. Iranian Fisheries Science Research Institute Published
- Published
- 2003
18. MODIFIED DIFFERENTIAL TRANSFORM METHOD FOR SINGULAR LANE-EMDEN EQUATIONS IN INTEGER AND FRACTIONAL ORDER.
- Author
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MARASI, H. R., SHARIFI, N., and PIRI, H.
- Subjects
MATHEMATICAL transformations ,POLYNOMIALS ,LANE-Emden equation - Abstract
In the present work the modified differential transform method, incorporating the Adomian polynomials into the differential transform method(DTM), is used to solve the nonlinear and singular Lane-Emden equations in integer and fractional order. Numerical examples with different types are solved. The results show that this method is very effective and simple. [ABSTRACT FROM AUTHOR]
- Published
- 2015
19. APPROXIMATING FIXED POINTS OF NONEXPANSIVE MAPPINGS AND SOLVING SYSTEMS OF VARIATIONAL INEQUALITIES.
- Author
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PIRI, H.
- Subjects
- *
FIXED point theory , *MATHEMATICAL mappings , *VARIATIONAL inequalities (Mathematics) , *APPROXIMATION theory , *STOCHASTIC convergence , *MATHEMATICS theorems - Abstract
A new approximation method for the set of common fixed points of nonexpansive mappings and the set of solutions of systems of variational inequalities is introduced and studied. More- over, we apply our main result to obtain strong convergence theorem to a common fixed point of a nonexpannsive mapping and solutions of a system of variational inequalities of an inverse strongly mono- tone mapping and strictly pseudo-contractive mapping of Browder- Petryshyn type. [ABSTRACT FROM AUTHOR]
- Published
- 2014
20. SOLUTIONS OF VARIATIONAL INEQUALITIES ON FIXED POINTS OF NONEXPANSIVE MAPPINGS.
- Author
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PIRI, H.
- Subjects
- *
VARIATIONAL inequalities (Mathematics) , *FIXED point theory , *NONEXPANSIVE mappings , *MONOTONE operators , *DIFFERENTIAL inequalities - Abstract
In this paper, we propose a generalized iterative method for finding a common element of the set of fixed points of a single nonexpannsive mapping and the set of solutions of two variational inequalities with inverse strongly monotone mappings and strictly pseudo-contractive of Browder-Petryshyn type mapping. Our results improve and extend the results announced by many others. [ABSTRACT FROM AUTHOR]
- Published
- 2013
21. AN ITERATIVE METHOD FOR AMENABLE SEMIGROUP AND INFINITE FAMILY OF NON EXPANSIVE MAPPINGS IN HILBERT SPACES.
- Author
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PIRI, H. and VAEZI, H.
- Subjects
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ITERATIVE methods (Mathematics) , *MATHEMATICAL mappings , *MATHEMATICAL inequalities , *HILBERT space , *STOCHASTIC convergence - Abstract
We introduce an iterative method for amenable semigroup of non expansive mappings and infinite family of non expansive mappings in the frame work of Hilbert spaces. We prove the strong convergence of the proposed iterative algorithm to the unique solution of a variational inequality, which is the optimality condition for a minimization problem. The results presented here mainly extend the corresponding results announced by Qin et al. and several others. [ABSTRACT FROM AUTHOR]
- Published
- 2012
22. FIXED POINT THEOREMS FOR GENERALIZED ; ' -WEAK CONTRACTIONS
- Author
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PIRI, H. and RAHROVI, S.
- Subjects
xed point,metric space,weak contractions,generalized,' -weak contrac- tions - Abstract
In this paper, we prove some xed point theorems for generalized ; ' - weak contractive mappings in a metric space. Our result generalized and extend recent results of Singh et al.[16, Theorem 2.1], Doric [7, Theorem 2.1], Rhoades [15, Theorem 1] and Dutta and Choudhary [9, Theorem 2.1]. Also, we provid an example to support the useability of our results.
23. Expression and purification of the recombinant kinase domain of FGFR2b and study of its structural changes Due to the interaction with gallic acid
- Author
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Seyyed-Attaran, F., Ilghari, D., Nematollah gheibi, Sahmani, M., and Piri, H.
24. The identification of active compounds and therapeutic properties of fermented and non-fermented red sorghum for the treatment of Alzheimer's dementia.
- Author
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Akbari M, Moardi S, Piri H, Amiri R, Aliaqabozorg F, and Afraz ES
- Subjects
- Animals, Male, Rats, Rats, Wistar, Flavanones, Gastrointestinal Microbiome, Disease Models, Animal, Flavonoids, Apigenin pharmacology, Phenols, Acetylcholine metabolism, Acetylcholinesterase metabolism, Anti-Inflammatory Agents pharmacology, Lactobacillus, Plant Extracts pharmacology, Feces microbiology, Feces chemistry, Sorghum, Alzheimer Disease microbiology, Alzheimer Disease metabolism, Fermentation, Antioxidants
- Abstract
Sorghum is a promising treatment for Alzheimer's disease (AD), due to its rich antioxidant and anti-inflammatory qualities. Fermentation may also affect nutritional values. Therefore, the purpose of this study was to discover the phenolic and flavonoid chemicals found in both fermented and non-fermented red sorghum, as well as their potential therapeutic uses for AD. L. fermentum, and L. reuteri, and/or L. plantarum and L. casei were used to ferment samples of sorghum. The rats were grouped into five groups, healthy animals, and rats with Alzheimer's receiving 200 mg/kg of saline, non-fermented sorghum, and fermented sorghum fermented with L. fermentum and L. reuteri, as well as L. plantarum and L. casei. Various assessments were conducted, including evaluations of behavioral responses, antioxidant responses, inflammatory responses, acetylcholine levels and acetylcholine esterase, and bacterial populations in stool. P-hydroxybenzoic acid, eriodictyo naringenin, and apigenin were significantly higher in fermented samples, while glycerols were higher in non-fermented samples. The induction of Alzheimer's led to decrease step-through latency, time in target zone, FRAP, acetylcholine levels, Bifidobacterium population and lactobacillus population, while increased escape latency, platform location latency, MDA levels, IL-6, TNF-α, acetylcholine esterase, and coliform population (P = 0.001). The administration of both non-fermented sorghum and fermented sorghum demonstrated the potential to reverse the effects of AD, with a notably higher efficacy observed in the fermented samples compared to the non-fermented ones. In conclusion, fermentation exerted significant effects on the bioactive compounds the administration of fermented sorghum resulted in improved behavioral responses, characterized by a reduction in oxidation, inflammation and microbial population., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2024
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25. Association Between the G82S Polymorphism of the Receptor Gene for Advanced Glycation End-products and Soluble Serum Levels RAGE with Diabetic Nephropathy in the White (Asian) Race.
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Rezaei Arnesa A, Hajian S, Salemi Bazargani S, Salahshourifar I, Moghbelinejad S, Abdolvahabi Z, Yaghoobzadeh H, Fathi M, and Piri H
- Subjects
- Aged, Female, Humans, Male, Middle Aged, Case-Control Studies, Genetic Predisposition to Disease, Polymorphism, Genetic, Polymorphism, Single Nucleotide, Asian People genetics, Diabetes Mellitus, Type 2 genetics, Diabetes Mellitus, Type 2 blood, Diabetes Mellitus, Type 2 complications, Diabetic Nephropathies genetics, Diabetic Nephropathies blood, Receptor for Advanced Glycation End Products genetics, Receptor for Advanced Glycation End Products blood
- Abstract
Introduction: Diabetic nephropathy is one of the most common severe symptoms of diabetes mellitus. Hyperglycemia can lead to tissue damage and inflammation due to mediators such as receptor for advanced glycation end-products (RAGE). Therefore, in this study, we aimed to investigate the association between the G82S polymorphism of the RAGE gene and diabetic nephropathy in diabetic patients., Methods: In this case-control study, 356 participants (158 men and 198 women) of Asian race, aged 45 to 65 years, who were diagnosed with type 2 diabetes mellitus based on their fasting plasma glucose levels were enrolled. DNA was isolated from the participants' blood samples and genotyped using TETRA -Primer ARMS-PCR. Serum protein concentration of soluble RAGE (sRAGE) was also determined by enzyme-linked immunosorbent assay (ELISA)., Results: Although we found differences in genotyping of participants between homozygous AA and GG and heterozygous GA in the studied groups, the differences were not significant (P = .568). In addition, we found no significant correlation between the G82S polymorphism of RAGE and the development of diabetic nephropathy. Serum levels of sRAGE were only slightly decreased in patients with diabetic nephropathy compared with diabetic patients (P > .05)., Conclusion: The results of this study indicate no significant association between the G82S polymorphism in the gene RAGE and the development of diabetic nephropathy. Serum levels of sRAGE were only slightly decreased in patients with diabetic nephropathy compared to diabetic patients without nephropathy. Therefore, the study suggests that there is probably no association between the G82S polymorphism in the gene RAGE and the development of diabetic nephropathy. DOI: 10.52547/ijkd.7872.
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- 2024
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26. The effect of various therapeutic exercises on forward head posture, rounded shoulder, and hyperkyphosis among people with upper crossed syndrome: a systematic review and meta-analysis.
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Sepehri S, Sheikhhoseini R, Piri H, and Sayyadi P
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- Humans, Exercise Therapy methods, Exercise, Posture physiology, Shoulder, Kyphosis therapy
- Abstract
Objectives: This review study aimed to evaluate the impact of therapeutic exercises on Upper-Crossed Syndrome (UCS). The study utilized a systematic review and meta-analysis approach to investigate the effects of various therapeutic exercises on forward head posture, rounded shoulders, and hyperkyphosis associated with upper crossed syndrome., Methods: The study identified relevant keywords for each independent and dependent variable and conducted a search in scientific databases, including PubMed, Web of Science, Scopus, and Google Scholar, without any time limitations until 12 August 2023. Overall, 4625 articles were found in the selected databases, which were reduced to 1085 after being entered into the EndNote software and removing duplicate data. The full texts of 30 remaining studies were reviewed; ten articles meeting the criteria were included. Additionally, 12 studies from the Google Scholar database were included, resulting in 22 studies. Using Comprehensive meta-analysis software (CMA ver 3), data heterogeneity was measured with I
2 and the Q tests. The Funnel Plot and Egger test methods were utilized to determine the possibility of publication bias. The JBI checklist was used to assess the quality of the studies., Results: The results of the meta-analysis showed that therapeutic exercises were effective in improving forward head, rounded shoulders, and thoracic kyphosis angles (CI 95% = -1.85-1.161, P = 0.001, P = 0.001, CI95%=-1.822-1.15, and P = 0.001, CI 95%= -1.83-1.09, respectively)., Conclusion: Based on the results, it appears that performing therapeutic exercises in the form of strength exercises, stretching, shoulder-based exercises, and incredibly comprehensive exercises that target all muscles may be effective in reducing forward head, rounded shoulders, thoracic kyphosis, and overall UCS., (© 2024. The Author(s).)- Published
- 2024
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27. Unraveling the function and structure impact of deleterious missense SNPs in the human OX1R receptor by computational analysis.
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Farajzadeh-Dehkordi M, Mafakher L, Harifi A, Haghdoost-Yazdi H, Piri H, and Rahmani B
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- Humans, Polymorphism, Single Nucleotide, Molecular Dynamics Simulation, Morphogenesis, Orexin Receptors, Behavior, Addictive, Nervous System Diseases
- Abstract
The orexin/hypocretin receptor type 1 (OX1R) plays a crucial role in regulating various physiological functions, especially feeding behavior, addiction, and reward. Genetic variations in the OX1R have been associated with several neurological disorders. In this study, we utilized a combination of sequence and structure-based computational tools to identify the most deleterious missense single nucleotide polymorphisms (SNPs) in the OX1R gene. Our findings revealed four highly conserved and structurally destabilizing missense SNPs, namely R144C, I148N, S172W, and A297D, located in the GTP-binding domain. Molecular dynamics simulations analysis demonstrated that all four most detrimental mutant proteins altered the overall structural flexibility and dynamics of OX1R protein, resulting in significant changes in the structural organization and motion of the protein. These findings provide valuable insights into the impact of missense SNPs on OX1R function loss and their potential contribution to the development of neurological disorders, thereby guiding future research in this field., (© 2024. The Author(s).)
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- 2024
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28. Classification and Diagnostic Prediction of Colorectal Cancer Mortality Based on Machine Learning Algorithms: A Multicenter National Study.
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Mohammadi G, Azizmohammad Looha M, Pourhoseingholi MA, Rezaei Tavirani M, Sohrabi S, Zareie Shab Khaneh A, Piri H, Alaei M, Parvani N, Vakilzadeh I, Javadi S, Moradian Haft Cheshmeh Z, Razzaghi Z, Robati RM, Zamanian Azodi M, Zarean Shahraki S, Hadavi M, Talebi R, Yazdani JC, Motlagh ME, and Khodakarim S
- Subjects
- Humans, Retrospective Studies, Bayes Theorem, Machine Learning, Algorithms, Colorectal Neoplasms diagnosis
- Abstract
Introduction: Colorectal cancer (CRC) ranks as the second leading cause of cancer-related deaths. This study aimed to predict survival outcomes of CRC patients using machine learning (ML) methods., Material and Methods: A retrospective analysis included 1853 CRC patients admitted to three prominent tertiary hospitals in Iran from October 2006 to July 2019. Six ML methods, namely logistic regression (LR), Naïve Bayes (NB), Support Vector Machine (SVM), Neural Network (NN), Decision Tree (DT), and Light Gradient Boosting Machine (LGBM), were developed with 10-fold cross-validation. Feature selection employed the Random Forest method based on mean decrease GINI criteria. Model performance was assessed using Area Under the Curve (AUC)., Results: Time from diagnosis, age, tumor size, metastatic status, lymph node involvement, and treatment type emerged as crucial predictors of survival based on mean decrease GINI. The NB (AUC = 0.70, 95% Confidence Interval [CI] 0.65-0.75) and LGBM (AUC = 0.70, 95% CI 0.65-0.75) models achieved the highest predictive AUC values for CRC patient survival., Conclusions: This study highlights the significance of variables including time from diagnosis, age, tumor size, metastatic status, lymph node involvement, and treatment type in predicting CRC survival. The NB model exhibited optimal efficacy in mortality prediction, maintaining a balanced sensitivity and specificity. Policy recommendations encompass early diagnosis and treatment initiation for CRC patients, improved data collection through digital health records and standardized protocols, support for predictive analytics integration in clinical decisions, and the inclusion of identified prognostic variables in treatment guidelines to enhance patient outcomes.
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- 2024
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29. Isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway.
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Asoudeh-Fard A, Salehi M, Ilghari D, Parsaei A, Heydarian P, and Piri H
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Objectives: It is worthwhile to note that, some probiotics such as Lactobacilli and Bifidobacteria isolated from dairy products have significant therapeutic effects against cancer cells. Here, we evaluated anti-proliferation and the apoptotic effects of isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products on the HeLa cervical cancer cells in vitro., Materials and Methods: The viability of treated HeLa cells with supernatant of Lactobacillus in 0.5, 0.75, 1, 1.5, and 2 ng/ml concentrations, and IC
50 values were detected by tetrazolium bromide. The L. fermentum Ab.RS22-induced cell death by flow cytometry was confirmed through evaluation of the expression of caspase-3 , P53 , PTEN , and AKT genes by quantitative reverse transcription-polymerase chain reactions (qRT-PCR)., Results: Most cytotoxicity effects of Lactobacillus on HeLa cells were detected in 2 ng/ml at 24 hr ( P <0.01); also, the IC50 value was measured as 1.5 ng/ml. The findings of the flow cytometry assay showed that L. fermentum Ab.RS22 in 1.5 ng/ml concentration at 24 hr increased the percentage of both apoptosis and necrosis cells. Lactobacillus-induced cell death was verified through results of Real-time PCR; where expression of caspase-3 , P53 , and PTEN genes was increased ( P <0.01), and also expression of AKT gene (anti-apoptotic) was decreased ( P <0.05)., Conclusion: Our findings showed that L. fermentum Ab.RS22 could dose-dependently inhibit the proliferation of the HeLa cells. Its apoptotic effect was confirmed via modulating PTEN/p53 / Akt gene expression and activation of the caspase-3 mediated apoptosis pathway. Therefore, L. fermentum Ab.RS22 can be considered a valuable anticancer candidate against cervical cancer progression in subsequent studies., Competing Interests: The authors declare that they have no conflicts of interest.- Published
- 2024
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30. The effect of dual-task on jump landing kinematics and kinetics in female athletes with or without dynamic knee valgus.
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Zamankhanpour M, Sheikhhoseini R, Letafatkar A, Piri H, Asadi Melerdi S, and Abdollahi S
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- Adolescent, Adult, Female, Humans, Young Adult, Athletes, Biomechanical Phenomena, Kinetics, Lower Extremity, Knee Joint
- Abstract
It has been indicated that dual tasks may multiply the possibility of injuries due to divided attention. This study aimed to investigate the effect of dual-task on kinematics and kinetics of jump landing in female athletes with and without dynamic knee valgus. In this study, 32 recreational athletes between 18 and 30 years old were recruited and divided into with (n = 17) and without (n = 15) dynamic knee valgus groups. The 3-D positions of retroreflective markers were recorded at 200 Hz using a 8-camera Kestrel system (Motion Analysis Corporation, Santa Rosa, CA), while ground reaction forces were synchronously recorded at 1000 Hz using 2 adjacent force plates (FP4060-NC; Bertec Corporation, Columbus, OH). Kinematics and kinetics of jump landing were recorded while counting backward digits as a dual task, and also without counting backward digits as a single task. One-way repeated measures of variance were used to analyse data at the significant level of 95% (α < 0.05). The study found that the dual-task affected the angles and moments of hip, knee, and ankle joints (P < 0.05) in both groups. Additionally, the effect of the dual-task differed significantly between the two groups in the angles hip flexion (P < 0.001), knee abduction (P < 0.001), and ankle internal rotation (P = 0.001), as well as the moments hip flexion (P < 0.001), hip abduction (P = 0.011), knee flexion (P = 0.017), knee internal rotation (P < 0.001), ankle dorsiflexion (P = 0.046), ankle eversion (P < 0.001), and ankle internal rotation (P = 0.046). Athletes with dynamic knee valgus may have been less able to protect themselves during the landing and are more prone to lower extremities injuries. As a result, using kinematics and kinetics in athletes with dynamic knee valgus during landing may help identify potential mechanisms associated with risk factors of lower extremity injuries and ACL injuries as well., (© 2023. Springer Nature Limited.)
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- 2023
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31. Association between physical activity and body posture: a systematic review and meta-analysis.
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Salsali M, Sheikhhoseini R, Sayyadi P, Hides JA, Dadfar M, and Piri H
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- Animals, Humans, Case-Control Studies, Cross-Sectional Studies, Exercise, Posture, Lordosis
- Abstract
Objective: To estimate the possible associations between posture and physical activity (PA)., Design: A systematic review and meta-analysis., Data Sources: The search was conducted in seven databases (PubMed, Web of Science, SportDiscus, EMBASE, Scopus, Cochrane Library, and CINAHL) for studies published from inception to January 2023., Eligibility Criteria for Selecting Studies: Studies were required to meet following criteria: (1) study design: cross-sectional, case control and cohort studies. (2) Participants: people of all ages without any diagnosed diseases. (3) Exposure and outcome: studies that examined the possible effect or correlations between PA, physical inactivity, physical exertion and human body posture., Results: Sixteen cross-sectional studies, two cohort studies and one case control study involving a total of 16772 participants aged from 6 to 79 years were included. Correlational studies showed that there was a significant relationship between PA and posture (C = 0.100, CI 95% = 0.012-0.186). However, regression studies demonstrated that there was not a significant association between PA and posture (C = 1.00, CI 95% = 0.998-1.002). Three studies investigated the association between PA and the lumbar lordosis and showed that there was not a significant association between the lordosis and PA (CI 95%: -0.253-0.048, P = 0.180). In addition, four studies showed that there were not any associations between scoliosis and PA (CI 95%: 0.819, 1.123, P = 0.607). The evidence of heterogeneity and publication bias was found among all analyzed data (P < 0.05). Also, meta regression was used for age and BMI and the results were not significant., Conclusion: Although a weak correlation was shown to exist between PA and human posture, the odds ratio indicated that there was not a significant association between PA and human posture. The lack of a significant relationship may indicate that multiple biopsychosocial factors may be involved in human posture. In summary, our study highlights the need for caution when interpreting the results of meta-analyses, particularly when there is significant heterogeneity and publication bias in the included studies., (© 2023. BioMed Central Ltd., part of Springer Nature.)
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- 2023
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32. The effects of exercise training on knee repositioning sense in people with knee osteoarthritis: a systematic review and meta-analysis of clinical trials.
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Sheikhhoseini R, Dadfar M, Shahrbanian S, Piri H, and Salsali M
- Subjects
- Humans, Aged, Exercise Therapy methods, Knee Joint, Exercise, Physical Therapy Modalities, Osteoarthritis, Knee therapy
- Abstract
Objective: Osteoarthritis (OA) of the knee is one of the most common global joint disorders, especially in aging population, and is among leading health-related concerns of societies. Therefore, this systematic review and meta-analysis was done to investigate the results related to the effects of exercise interventions on knee repositioning sense in patients with knee OA., Methods: An extensive search was independently performed in electronic databases including PubMed, MEDLINE, Web of Science, SCOPUS, and Google Scholar, to identify randomized clinical trials (RCTs) conducted on knee OA and to evaluate knee repositioning sense before and after different exercise interventions. After extracting relevant data from eligible studies, results of the studies were pooled using a random-effects model of meta-analysis. The Physiotherapy Evidence Database (PEDro) of clinical trials was used for quality assessment of eligible studies., Results: Among 2702 studies identified in the initial search, 17 studies were eligible for final systematic review and meta-analysis. The results showed that the patients who participated in different exercise interventions had significantly less knee repositioning error (mean differences: -1.141 degrees (95%CI: -1.510, -0.772, P < .001) compared to those who did not undergo exercise interventions. The eligible studies exhibited publication bias (Intercept: -6.69, P = .002), and the data showed significant heterogeneity (I2 = 85.633%, Q = 153.125, P < .001). Moreover, meta regression showed more prolonged exercise duration might have more effects on knee repositioning error (Coefficient=-0.860, 95% CI=-1.705, -0.016, Z=-2.00, P = .045)., Conclusion: There is strong evidence that exercise interventions may effectively reduce knee repositioning error. Moreover, it seems that more prolonged exercise duration may be associated with the greater effect size., (© 2023. The Author(s).)
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- 2023
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33. The Remarkable Roles of the Receptor for Advanced Glycation End Products (RAGE) and Its Soluble Isoforms in COVID-19: The Importance of RAGE Pathway in the Lung Injuries.
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Salehi M, Amiri S, Ilghari D, Hasham LFA, and Piri H
- Abstract
The respiratory symptoms of acute respiratory distress syndrome (ARDS) in the coronavirus disease 2019 (COVID-19) patients is associated with accumulation of pre-inflammatory molecules such as advanced glycation end-products (AGES), calprotectin, high mobility group box family-1 (HMGB1), cytokines, angiotensin converting enzyme 2 (ACE2), and other molecules in the alveolar space of lungs and plasma. The receptor for advanced glycation end products (RAGEs), which is mediated by the mitogen-activated protein kinase (MAPK), plays a critical role in the severity of chronic inflammatory diseases such as diabetes mellitus (DM) and ARDS. The RAGE gene is most expressed in the alveolar epithelial cells (AECs) of the pulmonary system. Several clinical trials are now being conducted to determine the possible association between the levels of soluble isoforms of RAGE (sRAGE and esRAGE) and the severity of the disease in patients with ARDS and acute lung injury (ALI). In the current article, we reviewed the most recent studies on the RAGE/ligands axis and sRAGE/esRAGE levels in acute respiratory illness, with a focus on COVID-19-associated ARDS (CARDS) patients. According to the research conducted so far, sRAGE/esRAGE measurements in patients with CARDS can be used as a powerful chemical indicator among other biomarkers for assessment of early pulmonary involvement. Furthermore, inhibiting RAGE/MAPK and Angiotensin II receptor type 1 (ATR1) in CARDS patients can be a powerful strategy for diminishing cytokine storm and severe respiratory symptoms., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interests., (© The Author(s), under exclusive licence to Association of Clinical Biochemists of India 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
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- 2023
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34. Rapid Detection and Identification of Fasciola spp. and Dicrocoelium spp. Isolated from the Ruminant Livestock of Northwest Iran Using High-Resolution Melting Analysis (HRM).
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Hajialilo E, Hosseini-Safa A, Spotin A, Saraei M, Ghanbari Johkool M, Piri H, and Heydarian P
- Abstract
Background: The liver flukes of the Fasciola species and Dicrocoelium spp. are recognised as parasites of domestic and wild herbivores. Both species of F. hepatica and F. gigantica as well as D. dendriticum are distributed in Iran. The present study aimed to identify Fasciola spp. and Dicrocoelium spp. using mitochondrial Cox1 (cytochrome c oxidase I) gene by HRM method., Methods: Totally, thirty infected liver specimens were collected from the sheep (n:23) and cattle (n:7) at the abattoirs of Qazvin Province, northwest Iran in 2022. DNA extraction and PCR amplification of Cox1 gene were conducted by HRM technique. DnaSP v.5.0 was used for compression of diversity indices of ribosomal 28S rDNA and mitochondrial Cox1 markers of Dicrocoelium spp. The taxonomic status of Dicrocoelium spp. was performed by sequencing and p hylogenetic analysis., Results: Overall, 26 and 4 isolates were identified as F. hepatica and F. gigantica , respectively. D. dendriticum was the sole infecting species of Dicrocoelium revealed by HRM analysis. Genomic analysis showed a moderate ( 28S rDNA genes: 0.600±0.215) to high ( Cox1 : 0.733±0.155) haplotype diversity for D. dendriticum ., Conclusion: The parasite-dependent mitochondrial gene ( Cox1 ) could identify a higher genetic diversity of D. dendriticum compared to nuclear 28S rDNA gene. HRM technique in the present study found to be a reliable technique for identification and genetic diversity of liver flukes but more comprehensive and in-depth studies in different parts of the country are needed., Competing Interests: Conflicts of interest The authors declare no conflicts of interest., (Copyright © 2023 Hajialilo et al. Published by Tehran University of Medical Sciences.)
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- 2023
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35. Biomolecular interactions and binding dynamics of inhibitor arachidonic acid, with tyrosinase enzyme.
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Shojazadeh T, Zolghadr L, JafarKhani S, Gharaghani S, Farasat A, Piri H, and Gheibi N
- Subjects
- Arachidonic Acid, Circular Dichroism, Thermodynamics, Monophenol Monooxygenase, Amino Acids
- Abstract
Hyperpigmentation is a disorder caused by increased melanin deposition and changes in skin pigmentation. Inhibition of tyrosinase activity contributes to the control of food browning and skin pigmentation diseases. The effects of arachidonic acid (AA) on tyrosinase activity were examined using different spectroscopy methods including UV-VIS spectrophotometry, fluorescence spectroscopy, circular dichroism (CD) differential scanning calorimetry, and molecular dynamics (MD) simulations. Based on the kinetic results, arachidonic acid showed mixed-type of inhibition with Ki = 4.7 µM. Fluorescence and CD studies showed changes of secondary and tertiary structures of enzyme and a reduction of α-helix* amino acids after its incubation with different concentrations of AA, which is also confirmed by DSSP analysis. In addition, differential scanning calorimetry (DSC) studies showed a decrease in thermodynamic stability of enzyme from Tm = 338.65k for sole enzyme after incubation with AA in comparison with complex enzyme with Tm= 334.26k, ΔH =7.52 kJ/mol, and ΔS = 0.15 kJ/mol k. Based on the theoretical methods, it was found that the interaction between enzyme and AA follows an electrostatic manner with ΔG = -8.314 kJ/mol and ΔH = -12.9 kJ/mol. The MD results showed the lowest flexibility in the complex amino acids and minimal fluctuations in AA interaction with tyrosinase in Residue 240 to 260 and 66 to 80. Thus, AA inhibitory and structural and thermodynamic instability of tyrosinase supported advantages of this fatty acid for prevention of medical hyperpigmentation. Therefore, it is a good candidate for cosmetic applications. Communicated by Ramaswamy H. Sarma.
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- 2023
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36. New insights into the inhibitory effect of phenol carboxylic acid antioxidants on mushroom tyrosinase by molecular dynamic studies and experimental assessment.
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Shojazadeh T, Zolghadr L, Gharaghani S, JafarKhani S, Molaabasi F, Piri H, and Gheibi N
- Subjects
- Antioxidants, Molecular Docking Simulation, Molecular Dynamics Simulation, Phenol, Carboxylic Acids, Enzyme Inhibitors chemistry, Circular Dichroism, Monophenol Monooxygenase, Agaricales
- Abstract
The inhibitory effects of ferulic and chlorogenic acids on tyrosinase activity were investigated through multi-spectroscopic and molecular docking techniques. Ferulic and chlorogenic acids, flavonoid compounds, demonstrated inhibitory monophenolase activities of tyrosinase. The inhibitor effects against monophenolase activity were in a reversible and competitive manner with ki value equal to 6.8 and 7.5 µM respectively. The affinity between tyrosinase and L-DOPA decreased when fatty acids were added to the solution. The multi-spectroscopic techniques like UV-vis, fluorescence, and isothermal calorimetry are employed to investigate changes. Intrinsic fluorescence quenching and conformational changes of tyrosinase by hydrophobic interaction were confirmed. Tyrosinase had two and three binding sites for ferulic and chlorogenic acids with a binding constant in the order of magnitude of -6.8 and -7.2 kcal/mol. In addition, the secondary structural changes with Circular dichroism (CD) analysis, secondary structure (DSSP), radius of gyration (Rg) and analysis of hydrogen bonds (H-bonds) confirmed. Ferulic acid effect can be observed obviously and also content of α-helix decreased. Thermodynamic parameters indicated that the interaction between enzyme and ferulic and chlorogenic acids followed a spontaneous reaction dynamic manner with ΔG = -14.78 kJ/mol and ΔG = -14.61 kJ/mol (298k). The findings highlighted the potential applications of ferulic acid and chlorogenic acids in food and drug industries as potent inhibitors of tyrosinase.Communicated by Ramaswamy H. Sarma.
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- 2023
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37. Mortality among hospitalized COVID-19 patients during surges of SARS-CoV-2 alpha (B.1.1.7) and delta (B.1.617.2) variants.
- Author
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Zali A, Khodadoost M, Gholamzadeh S, Janbazi S, Piri H, Taraghikhah N, Hannani K, Looha MA, and Mohammadi G
- Subjects
- Humans, Iran epidemiology, Hospitalization, Hospital Mortality, Retrospective Studies, SARS-CoV-2, COVID-19 epidemiology
- Abstract
The aim of this study was to evaluate the death proportion and death risk of COVID-19 hospitalized patients over time and in different surges of COVID-19. This multi-center observational study was conducted from March 21, 2021 to October 3, 2021 which included the alpha and delta SARS-CoV-2 surges occurred in April and August in Tehran, respectively. The risk of COVID-19 death was compared in different months of admission. A total of 270,624 patients with COVID-19, of whom 6.9% died, were admitted to hospitals in Tehran province. Compared to patients admitted in March, a higher risk of COVID-19 death was observed among patients admitted to the hospital in July (HR 1.28; 95% CI 1.17, 1.40), August (HR 1.40; 95% CI 1.28, 1.52), September (HR 1.37; 95% CI 1.25, 1.50) and October (HR 4.63; 95% CI 2.77, 7.74). The ICU death proportion was 36.8% (95% CI: 35.5, 38.1) in alpha surge and increased significantly to 39.8 (95% CI 38.6, 41.1) in delta surge. The risk of COVID-19 death was significantly higher in delta surge compared to alpha surge (HR 1.22; 95% CI 1.17, 1.27). Delta surge was associated with a higher risk of death compared to alpha surge. High number of hospitalizations, a shortage of hospital beds, ICU spaces and medical supplies, poor nutritional status of hospitalized patients, and lack of the intensivist physicians or specialized nurses in the ICU were factors that contributed to the high mortality rate in the delta surge in Iran., (© 2022. The Author(s).)
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- 2022
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38. Structural and inhibitory effects of fulvic and humic acids against tyrosinase.
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Taherkhani N, Hekmat A, Piri H, and Haghbeen K
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- Amino Acids, Animals, Antioxidants, Lipids, Mammals, Molecular Docking Simulation, Humic Substances analysis, Monophenol Monooxygenase
- Abstract
Inhibition of tyrosinase activity can control fruit browning and preserve the flavor and nutritional value of food. The impacts of fulvic acid (FA) and humic acid (HA) on tyrosinase activity were investigated utilizing circular dichroism (CD) and fluorescence spectroscopy, molecular docking (MD), and molecular dynamics simulations. HA and FA demonstrated a mixed type of inhibition with K
i 2.02 and 5.2 μM, respectively. The thermodynamic parameters displayed that the hydrogen bond and hydrophobic force play a major role in the FA-tyrosinase and HA-tyrosinase interaction, respectively. Fluorescence experiments demonstrated changes in tyrosinase tertiary structures. HA could not destroy the tyrosinase secondary structure significantly, however, FA has a significant influence on the tyrosinase secondary structure. The molecular dynamics findings demonstrated the minimal fluctuations and the lowest flexibility in the complex amino acids in the HA-tyrosinase and FA-tyrosinase interaction. Altogether, HA and FA could be utilized in food industries as an accessible natural source for tyrosinase inhibition. PRACTICAL APPLICATIONS: Recently, the investigation of tyrosinase inhibitors from the biosphere for hindrance of undesired browning in the food industry has increased considerably. Mushroom tyrosinase is a suitable model for kinetic research owing to its availability as well as close conformational similarity to tyrosinase in a mammal. Natural sources and their effective compounds could have wonderful potential on tyrosinase activity and structure, thus, in this study, the interactions between tyrosinase and fulvic acid (FA) and Humic acid (HA) were investigated. Previously, it has been shown that HA and FA have antioxidant properties and they can improve the quality of food via retarding lipid oxidation. Altogether, further investigations are warranted to draw firm conclusions, HA and FA could be utilized in food industries not only as antioxidant agents but also as an accessible natural source for tyrosinase inhibition., (© 2022 Wiley Periodicals LLC.)- Published
- 2022
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39. Correction: Establishing minimal clinically important difference for effectiveness of corrective exercises on craniovertebral and shoulder angles among students with forward head posture: a clinical trial study.
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Heydari Z, Sheikhhoseini R, Shahrbanian S, and Piri H
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- 2022
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40. The Association of Oxidative Stress and Reactive Oxygen Species Modulator 1 (ROMO1) with Infertility: A Mini Review.
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Amini MA, Karimi M, Talebi SS, Piri H, and Karimi J
- Abstract
Infertility is one of the disorders that worries many couples around the world, although novel and molecular methods can be used to cure this disease in different stages. One of the factors that causes infertility in men and women is the increased oxidative stress within the cells, which can lead to damage in zygote formation. ROMO1 is one of the most important proteins in the production of reactive oxygen species. This protein can enhance oxidative stress in the cells and body through cellular pathways, such as TNF-α and NF-κB routes, which will eventually lead to many diseases, especially infertility. We engage several international databases by using keywords; ROMO1, Infertility, and Reactive Oxygen Species, and gained a great quantity of information about ROMO1, Infertility, and Oxidative Stress. Although not proven, it is hypothesized that ROMO1 might elevate oxidative stress by activating NF-κB pathway in the cells, furthermore, TNF-α can arouse ROMO1 that can end up with apoptosis and cell death, which consequently can have a lot of disturbing effects on the body, especially the reproductive system. To sum up, revealing the exact cellular and molecular mechanisms of ROMO1-dependent TNF-α and NF-κB pathways in the pathogenesis of infertility might find interesting therapeutic and management strategies for this disorder., Competing Interests: CONFLICT OF INTEREST STATEMENT: None declared., (© Chonnam Medical Journal, 2022.)
- Published
- 2022
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41. Dopaminergic neuronal death in the substantia nigra associates with change in serum levels of TNF-α and IL-1β; evidence from early experimental model of Parkinson's disease.
- Author
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Piri H, Sharifi S, Nigjeh S, and Haghdoost-Yazdi H
- Subjects
- Animals, Cytokines metabolism, Disease Models, Animal, Dopamine, Oxidopamine toxicity, Rats, Substantia Nigra pathology, Dopaminergic Neurons cytology, Dopaminergic Neurons pathology, Interleukin-1beta blood, Parkinson Disease drug therapy, Tumor Necrosis Factor-alpha blood
- Abstract
Purpose: Studies have shown that inflammation plays a key role in etiology of Parkinson's disease (PD). However, human studies which have evaluated association between PD and serum levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) have reported conflicting results. In this study, serum and striatum levels of these cytokines were evaluated in 6-hydroxydopamine (6-OHDA) animal model of PD., Method: The neurotoxin of 6-OHDA was injected into medial forebrain bundle of right hemisphere and behavioral tests were carried out to eight weeks thereafter to evaluate severity of PD and its progress. Blood was collected before the toxin and in second and eight weeks after that. Survival of dopaminergic (DAergic) neurons in substantia nigra was assessed by immunohistochemistry. TNF-α and IL-1β levels were determined using ELISA kits., Result: Severity of behavioral symptoms was gradually increased in 6-OHDA-treated rats. They showed a decrease in serum TNF-α level in the eight week and increase in IL-1β both in the second and eight weeks. They were divided into two subgroups, symptomatic and asymptomatic with severe and moderate degrees in DAergic neuronal death. Significant decrease in serum TNF-α was only observed in the symptomatic subgroup but IL-1β increased in both subgroups. Also, striatal levels of both cytokines were higher in the lesioned hemisphere., Conclusion: Increase in serum IL-1β level can reflect moderate degree of lesion in substantia nigra and thereby is used for prognosis of PD before its clinical symptoms are appeared. On the other hand, an increase in serum TNF-α is appeared in advanced stage of PD.
- Published
- 2022
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42. Establishing minimal clinically important difference for effectiveness of corrective exercises on craniovertebral and shoulder angles among students with forward head posture: a clinical trial study.
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Heydari Z, Sheikhhoseini R, Shahrbanian S, and Piri H
- Subjects
- Exercise Therapy methods, Head, Humans, Male, Neck, Posture, Students, Minimal Clinically Important Difference, Shoulder
- Abstract
Background: Previous studies have addressed the effects of different exercises and modalities on forward head posture (FHP), but the minimal clinically important difference (MCID) of the effect of exercises on FHP remains unclear. Therefore, this study aimed to investigate the effects of selective corrective exercises (SCEs) on the craniovertebral angle (CVA) and shoulder angle (SA) in students with FHP and to establish MCID for these angles., Methods: In this randomized clinical trial study, a total of 103 second-grade male students with FHP were enrolled. Participants were randomly assigned to experimental and control groups. CVA and SA of participants were measured before and after the 8-week selective corrective exercise program (including strengthening and stretching exercises). The photogrammetric method was used to measure CVA and SA. MCID value was calculated for CVA and SA using the distribution method., Results: The results showed that there was a significant difference between the experimental and control groups in terms of CVA (F = 89.04, P = 0.005, Effect size = 0.47) and SA (F = 18.83, P = 0.005, Effect size = 0.16). After eight weeks of selective corrective exercises, the MCID values of CVA and SA were 1.40° and 1.34°, respectively., Conclusion: This study revealed that the selective corrective exercises might lead to postural correction of students having FHP problem. Results further indicated that a corrective exercise program would be considered beneficial if it increased CVA and SA values at least 1.40 and 1.34 degrees, respectively., (© 2022. The Author(s).)
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- 2022
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43. Activation of apoptosis and G0/G1 cell cycle arrest along with inhibition of melanogenesis by humic acid and fulvic acid: BAX/BCL -2 and Tyr genes expression and evaluation of nanomechanical properties in A375 human melanoma cell line.
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Salehi M, Piri H, Farasat A, Pakbin B, and Gheibi N
- Abstract
Objectives: Humic acid (HA) and Fulvic acid (FA) are major members of humic substances, which are extracted from organic sources including soil and peat. The pro-apoptotic and anti-melanogenic effects of HA and FA at the cellular and molecular levels in the A375 human melanoma cell line were examined in this study., Materials and Methods: The cytotoxicity effect of HA and FA were evaluated by cell viability assay. Apoptosis and cell cycle were investigated by flow cytometry. Real-time PCR was carried out to measure the expression of BAX , BCL-2, and Tyr genes. Moreover, the changes in nanomechanical properties were determined through atomic force microscopy (AFM)., Results: It was found that HA and FA decrease cell viability with an IC
50 value of 50 µg/ml (dose-dependent) for 14 hr, arrested cells in the G0/G1 phase, and increased the sub-G1 phase (induce apoptosis). Based on the AFM analysis, Young's modulus and adhesion force values were increased, also ultrastructural characteristics of cells were changed. Results of Real-time PCR revealed that HA and FA lead to a decrease in the expressions of BCL-2 and Tyr genes, and increase the BAX gene expression., Conclusion: These results exhibited that HA and FA possess pro-apoptotic effects through increasing the BAX/ BCL-2 expression in A375 cells. These molecular reports were confirmed by cellular nanomechanical assessments using AFM and flow cytometry. In addition, HA and FA inhibited melanogenesis by decreasing the expression of the Tyr gene. It is worthwhile to note that, HA and FA can be regarded to design new anti-cancer and anti-melanogenesis products., Competing Interests: The authors of this study have no conflicts of interests.- Published
- 2022
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44. The Association of COVID-19 and Reactive Oxygen Species Modulator 1 (ROMO1) with Oxidative Stress.
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Amini MA, Karimi J, Talebi SS, and Piri H
- Abstract
There is no denying that the massive spread of COVID-19 around the world has worried everyone. The virus can cause mild to severe symptoms in various organs, especially the lungs. The virus affects oxidative stress in the cells. Reactive Oxygen Species modulator 1 (ROMO1) is one of the most important mitochondrial proteins that plays a critical regulatory role in the production of Reactive Oxygen Species (ROS). According to the studies, COVID-19 can promote oxidative stress through some important pathways, for instance, TNF-α and NF-κB routes. Furthermore, ROMO1 is closely related to these pathways and its dysfunction may affect these routes, then promote oxidative stress, and ultimately cause tissue damage, especially in the lungs. Another factor to consider is that the TNF-α and NF-κB pathways are associated with ROMO1, COVID-19, and oxidative stress. To summarize, it is hypothesized that COVID-19 may increase oxidative stress by affecting ROMO1. Understanding the exact molecular mechanisms of ROMO1 in the pathogenesis of COVID-19 can pave the way to find better therapeutic strategies., Competing Interests: CONFLICT OF INTEREST STATEMENT: None declared., (© Chonnam Medical Journal, 2022.)
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- 2022
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45. Evaluation of the Association Between Serum Levels of Testosterone and Prolactin With 6- Hydroxydopamine-Induced Parkinsonism in Male Rats.
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Razaghi R, Piri H, Jafari H, Rastgoo N, Hosseini MA, and Haghdoost Yazdi H
- Abstract
Introduction: Parkinson's Disease (PD) associates with changes in sex hormones; however, it remains unknown whether this is either a cause for or a result of the disease. To further evaluate it, we investigated if the development of 6-Hydroxydopamine (6-OHDA)-induced Parkinsonism changes the serum levels of testosterone and prolactin or not., Methods: 6-OHDA was injected into the medial forebrain bundle using stereotaxic surgery. The development of Parkinsonism was evaluated by apomorphine-induced rotational test and the immunofluorescence labeling of Dopaminergic (DA) neurons in substantia nigra. The necessary blood samples were collected before the toxin and in the third and sixth weeks afterward. The hormones levels were determined using Enzyme-Linked Immunosorbent Assay (ELISA) kits., Results: The severity of rotations was different among 6-OHDA-treated rats; accordingly, they were divided into two subgroups of severe and mild parkinsonian rats. The degeneration of DA neurons was observed in both subgroups; however, it was significantly less in the mild group. In the sixth week after the toxin, testosterone level increased but only in the severe subgroup. Prolactin increased in both subgroups in the third week after the toxin but returned to normal in the sixth week. There was no association between the pre-toxin levels of these hormones and the intensity of Parkinsonism., Conclusion: Our findings indicated that the development of 6-OHDA-induced Parkinsonism increases the serum levels of testosterone and prolactin. Increased prolactin occurred earlier and was observed in rats with less DA neuronal loss. Therefore, prolactin levels can predict the death of DA neurons before the clinical signs of PD were revealed., Competing Interests: Conflict of interest The authors declared no conflicts of interest., (Copyright© 2021 Iranian Neuroscience Society.)
- Published
- 2021
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46. Effectiveness of booster dose of tetanus and diphtheria toxoids (Td) vaccine in management of recurrent aphthous stomatitis: a prospective, randomized, triple-blind and placebo-controlled clinical trial.
- Author
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Habibzadeh S, Sheikh Rahimi M, Edalatkhah H, Piri H, and Maleki N
- Subjects
- Adult, Colchicine therapeutic use, Diphtheria-Tetanus Vaccine immunology, Double-Blind Method, Female, Follow-Up Studies, Humans, Immunization, Secondary, Male, Middle Aged, Placebo Effect, Prospective Studies, Recurrence, Treatment Outcome, Vitamin B 6 therapeutic use, Young Adult, Diphtheria-Tetanus Vaccine administration & dosage, Stomatitis, Aphthous therapy
- Abstract
Background: Recurrent aphthous stomatitis (RAS) is the most common oral disease. The activation of the immune system by vaccines might reduce the interactions between oral mucosa and microorganisms., Aim: To evaluate the effect of the tetanus-diphtheria toxoids (Td) vaccine in management of RAS., Design and Setting: This prospective, randomized, triple-blind and placebo-controlled clinical trial study was conducted on 70 eligible patients with minor RAS at the dermatology outpatient clinic., Method: Finally, a total of 66 participants (48 male, 18 female; mean age: 38.56 ± 10.98 years) completed the study in two groups, one in which colchicine and a single dose of vitamin B6 (placebo group) was treated and one in which colchicine and a single dose of Td vaccine (intervention group) was treated., Results: After six months of follow-up, the patients were evaluated, which revealed significant effects of the Td vaccine on pain intensity, ulcer size, recovery time, and the interval between episodes. At the end of the six month follow-up, 27 patients (81.8%) in the intervention group and 13 patients (39.4%) in the placebo group showed partial or complete recovery, and there was statistically significant difference between the groups ( p < .001). Recovery was not significantly associated with sex, education level, marital status and duration of RAS. However, occupation and positive family history of RAS had significant relations with recovery., Conclusions: A booster dose of Td vaccine had relatively favorable effects on pain intensity and recurrence of RAS, but further research is needed to confirm its efficacy.
- Published
- 2021
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47. The fabrication of a novel film based on polycaprolactone incorporated with chitosan and rutin: potential as an antibacterial carrier for rainbow trout packaging.
- Author
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Piri H, Moradi S, and Amiri R
- Abstract
Abstract: Rutin and chitosan could be utilized in the food industry owing to their antioxidant and antibacterial properties. This study was carried out to fabricate novel films using polycaprolactone (PCL-sole), PCL and chitosan (PCL-CS), PCL and rutin (PCL-R), and PCL, chitosan, and rutin (PCL-CS-R) through electros pinning method. Physical properties, in vitro antibacterial and antioxidant properties of the films, and their antibacterial activity on rainbow trout were further investigated. The PCL-CS, PCL-R, and PCL-CS-R had smaller fiber diameter and film thickness and lower viscosity while they showed higher surface tension, water contact angle, and conductivity and better antibacterial and antioxidant properties compared with PCL-sole film ( P < 0.05). The PCL-CS-R film respectively decreased 17.45%, 19.27%, and 18.39% more populations of L. monocytogenes , S. aureus , and E. coli compared to PCL-sole film in the fish samples. Therefore, the PCL-CS-R film can be potentially used in active packaging because of its antioxidant and antibacterial activities., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© The Korean Society of Food Science and Technology 2021.)
- Published
- 2021
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48. The Anti-Parkinsonism Effects of K ATP Channel Blockade in the 6-Hydroxydopamine-Induced Animal Model: The Role of Oxidative Stress.
- Author
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Piri H, Haghdoost-Yazdi H, Fraidouni N, Dargahi T, Yaghoubidoust M, and Azadmehr A
- Abstract
Introduction: Studies suggest that ATP-sensitive potassium (K
ATP ) channels are a potential pharmacotherapeutic target for neuroprotection in neurodegenerative diseases. The current study aimed at evaluating the effect of pretreatment with glibenclamide (Glib) and B vitamins supplement on the severity of behavioral symptoms in 6-hydroxydopamine (OHDA)-induced Parkinsonism. Also malondialdehyde (MDA) concentration was measured in the blood and brain suspensions to find probable neuroprotective mechanism of Glib., Methods: The 6-OHDA was injected into striatum of rats by stereotaxic surgery. Treatment with Glib and B vitamins was started before the surgery and continued up to 3 weeks after that. Development and severity of Parkinsonism were evaluated by conventional behavioral tests. MDA values were measured spectrophotometrically using thiobarbituric acid and MDA standard curve., Results: Pretreatments with Glib, at both doses of 1 and 5 mg/kg or B vitamins significantly ameliorated severity of the behavioral symptoms. Pretreatment with a combination of Glib and B vitamins was more effective than pretreatment with Glib or B vitamins alone. Also, pretreatment with B vitamins, Glib, or a combination of them reduced MDA concentration in the brain suspensions. Decrease in MDA concentration in the group of rats that received a combination of B vitamins and Glib was more prominent than that of the Glib groups., Conclusion: As severity of the behavioral symptoms in the 6-OHDA-induced Parkinsonism reflects the degree of the lesion in Substantia Nigra (SN) dopaminergic neurons, it is suggested that Glib pretreatment has neuroprotective effect against 6-OHDA-induced neurotoxicity. The current study data also showed that this effect may be mediated by antioxidant effect of Glib., Competing Interests: Conflict of Interest The authors declared no conflicts of interest.- Published
- 2017
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49. Blockade of fast A-type and TEA-sensitive potassium channels provide an antiparkinsonian effect in a 6-OHDA animal model.
- Author
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Haghdoost-Yazdi H, Piri H, Najafipour R, Faraji A, Fraidouni N, Dargahi T, and Alipour Heidari M
- Subjects
- Animals, Apomorphine antagonists & inhibitors, Apomorphine pharmacology, Dose-Response Relationship, Drug, Drug Synergism, Male, Malondialdehyde blood, Medial Forebrain Bundle drug effects, Neuroprotective Agents pharmacology, Parkinson Disease, Secondary blood, Rats, Rotarod Performance Test, 4-Aminopyridine pharmacology, Antiparkinson Agents pharmacology, Oxidopamine, Parkinson Disease, Secondary chemically induced, Parkinson Disease, Secondary prevention & control, Potassium Channel Blockers pharmacology, Tetraethylammonium pharmacology
- Abstract
Objective: To evaluate the effect of K+ channels inhibitors in treatment of parkinson`s disease (PD)., Methods: This prospective comparative study was conducted in the Qazvin University of Medical Sciences, Iran, from April 2015 to January 2016. Male rats (n=37) received intraperitoneal doses of TEA (2 and 5 mg/kg) or 4-AP (0.5 and 1 mg/kg) twice-daily, before a stereotactic injection of 6-hydroxydopamine (6-OHDA) for the following 7 days. The 6-OHDA was injected into right medial forebrain bundle (MFB) of the rat brains. Development and severity of PD were assessed using the apomorphine-induced rotational test, the elevated body swing test and rotarod tests. Concentration of malondialdehyde (MDA), a marker of oxidative stress, was measured in rat sera., Results: Tetraethylammonium and 4-AP significantly reduced the number of apomorphine-induced rotations and improved motor learning in the rotarod test at both doses. Administration of 4-AP and TEA together was more effective than single administration of either agent. Malondialdehyde measurement showed that pretreatment with TEA could not prevent 6-OHDA-induced oxidative stress., Conclusion: Our results showed that pretreatment with TEA and 4-AP has a neuroprotective effect against 6-OHDA in dopaminergic neurons in the substantia nigra.
- Published
- 2017
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50. Pretreatment with potassium channel blockers of 4-aminopyridine and tetraethylammonium attenuates behavioural symptoms of Parkinsonism induced by intrastriatal injection of 6-hydroxydopamine; the role of lipid peroxidation.
- Author
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Haghdoost-Yazdi H, Piri H, Faraji A, Fraidouni N, Dargahi T, Mahmudi M, and Alipour Heidari M
- Subjects
- Adrenergic Agents, Animals, Apomorphine pharmacology, Corpus Striatum drug effects, Disease Models, Animal, Dose-Response Relationship, Drug, Drug Administration Schedule, Functional Laterality drug effects, Male, Motor Activity drug effects, Oxidopamine toxicity, Parkinsonian Disorders blood, Parkinsonian Disorders chemically induced, Postural Balance drug effects, Rats, Rats, Wistar, Thiazolidinediones blood, 4-Aminopyridine therapeutic use, Mental Disorders etiology, Mental Disorders prevention & control, Parkinsonian Disorders complications, Potassium Channel Blockers therapeutic use, Tetraethylammonium therapeutic use
- Abstract
Objectives: Potassium channels participate in cellular and molecular signalling pathways regulating the life and death of neurons. In this study, effect of pretreatment with potassium channel blockers of 4-aminopyridine (4-AP) and tetraethylammonium (TEA) on the behavioural symptoms of 6-hydroxydopamine (6-OHDA)-induced Parkinsonism was evaluated., Methods: 6-OHDA was injected into right striatum of adult male Wistar rats using stereotaxic surgery. Severity of 6-OHDA-induced Parkinsonism was assessed by conventional behavioural tests. 4-AP and TEA were injected twice per day intraperitoneally, before 6-OHDA injection to 15 days after that. Also, malondialdehyde (MDA) concentration as a marker of oxidative stress was measured in rat sera., Results: Pretreatment with 4-AP (0.5 and 1 mg/kg) and TEA (2 and 5 mg/kg) attenuated significantly behavioural symptoms of 6-OHDA-induced Parkinsonism. Application of both 4-AP and TEA together was more effective than the effect of each one of these blockers alone. 6-OHDA increased MDA concentration but pretreatment with 4-AP prevented of 6-OHDA-induced increase in MDA. On the other hand, pretreatment with TEA and combination of TEA and 4-AP could not prevent of 6-OHDA-induced oxidative stress., Discussion: Since severity of behavioural symptoms of 6-OHDA-induced Parkinsonism is correlated to the degree of nigral dopaminergic cell death, we suggest that antiparkinsonism effect of TEA and 4-AP was mediated by their neuroprotective effect. Because, both Parkinsonism in rat and PD in human, the main pathophysiological hallmark, is neurodegeneration of dopaminergic neurons in substantia nigra, we suggest doing clinical trials for evaluation of effectiveness of 4-AP and TEA in slowing down of PD progress.
- Published
- 2016
- Full Text
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