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8 results on '"Pineda-Castañeda, Héctor Manuel"'

5. Chimeric Peptides Derived from Bovine Lactoferricin and Buforin II: Antifungal Activity against Reference Strains and Clinical Isolates of Candida spp.

Author

Aguirre-Guataqui, Katherine, primary, Márquez-Torres, Mateo, additional, Pineda-Castañeda, Héctor Manuel, additional, Vargas-Casanova, Yerly, additional, Ceballos-Garzon, Andrés, additional, Rivera-Monroy, Zuly Jenny, additional, García-Castañeda, Javier Eduardo, additional, and Parra-Giraldo, Claudia Marcela, additional

Published
2022
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8. Synthetic Peptide Purification via Solid-Phase Extraction with Gradient Elution: A Simple, Economical, Fast, and Efficient Methodology.

Author

Insuasty Cepeda, Diego Sebastián, Pineda Castañeda, Héctor Manuel, Rodríguez Mayor, Andrea Verónica, García Castañeda, Javier Eduardo, Maldonado Villamil, Mauricio, Fierro Medina, Ricardo, Rivera Monroy, Zuly Jenny, and Samanidou, Victoria

Subjects
*ETHNOMETHODOLOGY, *DISCOURSE analysis, *AMINO acids, *C-terminal residues, *PERFORMANCE anxiety
Abstract

A methodology was implemented for purifying peptides in one chromatographic run via solid-phase extraction (SPE), reverse phase mode (RP), and gradient elution, obtaining high-purity products with good yields. Crude peptides were analyzed by reverse phase high performance liquid chromatography and a new mathematical model based on its retention time was developed in order to predict the percentage of organic modifier in which the peptide will elute in RP-SPE. This information was used for designing the elution program of each molecule. It was possible to purify peptides with different physicochemical properties, showing that this method is versatile and requires low solvent consumption, making it the least polluting one. Reverse phase-SPE can easily be routinely implemented. It is an alternative to enrich and purified synthetic or natural molecules. [ABSTRACT FROM AUTHOR]

Published
2019
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