5 results on '"Pierluigi G. Manti"'
Search Results
2. The Transcriptional Regulator Prdm1 Is Essential for the Early Development of the Sensory Whisker Follicle and Is Linked to the Beta-Catenin First Dermal Signal
- Author
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Pierluigi G. Manti, Fabrice Darbellay, Marion Leleu, Aisling Y. Coughlan, Bernard Moret, Julien Cuennet, Frederic Droux, Magali Stoudmann, Gian-Filippo Mancini, Agnès Hautier, Jessica Sordet-Dessimoz, Stephane D. Vincent, Giuseppe Testa, Giulio Cossu, and Yann Barrandon
- Subjects
sensory vibrissae ,barrel cortex ,Prdm1 ,Lef1 ,Leaf ,non-conserved enhancer ,Biology (General) ,QH301-705.5 - Abstract
Prdm1 mutant mice are one of the rare mutant strains that do not develop whisker hair follicles while still displaying a pelage. Here, we show that Prdm1 is expressed at the earliest stage of whisker development in clusters of mesenchymal cells before placode formation. Its conditional knockout in the murine soma leads to the loss of expression of Bmp2, Shh, Bmp4, Krt17, Edar, and Gli1, though leaving the β-catenin-driven first dermal signal intact. Furthermore, we show that Prdm1 expressing cells not only act as a signaling center but also as a multipotent progenitor population contributing to the several lineages of the adult whisker. We confirm by genetic ablation experiments that the absence of macro vibrissae reverberates on the organization of nerve wiring in the mystacial pads and leads to the reorganization of the barrel cortex. We demonstrate that Lef1 acts upstream of Prdm1 and identify a primate-specific deletion of a Lef1 enhancer named Leaf. This loss may have been significant in the evolutionary process, leading to the progressive defunctionalization and disappearance of vibrissae in primates.
- Published
- 2022
- Full Text
- View/download PDF
3. The Transcriptional Regulator
- Author
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Pierluigi G, Manti, Fabrice, Darbellay, Marion, Leleu, Aisling Y, Coughlan, Bernard, Moret, Julien, Cuennet, Frederic, Droux, Magali, Stoudmann, Gian-Filippo, Mancini, Agnès, Hautier, Jessica, Sordet-Dessimoz, Stephane D, Vincent, Giuseppe, Testa, Giulio, Cossu, and Yann, Barrandon
- Published
- 2022
4. Reconstitution of a functional human thymus by postnatal stromal progenitor cells and natural whole-organ scaffolds
- Author
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Roberta Ragazzini, Luca Zanieri, Pierluigi G. Manti, Hans J. Stauss, Adrian Hayday, Constance Maurer, Sarah A. Teichmann, Stefan Boeing, Giuseppe Testa, Gianluca Vozza, Miguel Muñoz-Ruiz, John C Hutchinson, Sara Campinoti, Demetra Ellie Phylactopoulos, Dominique Bonnet, Neil J. Sebire, Paola Bonfanti, Linda Ariza-McNaughton, Marco Catucci, Jong-Eun Park, Carlo Emanuele Villa, Asllan Gjinovci, Campinoti, Sara [0000-0003-0056-5792], Ragazzini, Roberta [0000-0003-2186-293X], Zanieri, Luca [0000-0001-8889-3058], Hutchinson, John C [0000-0002-1708-2634], Manti, Pierluigi G [0000-0003-0110-8251], Vozza, Gianluca [0000-0003-3562-4457], Maurer, Constance [0000-0002-9720-0118], Testa, Giuseppe [0000-0002-9104-0918], Stauss, Hans J [0000-0003-4340-7911], Teichmann, Sarah A [0000-0002-6294-6366], Hayday, Adrian C [0000-0002-9495-5793], Bonnet, Dominique [0000-0002-4735-5226], Bonfanti, Paola [0000-0001-9655-3766], and Apollo - University of Cambridge Repository
- Subjects
0301 basic medicine ,Male ,General Physics and Astronomy ,Autoimmunity ,Mice ,0302 clinical medicine ,Chemical Biology & High Throughput ,education.field_of_study ,Human Biology & Physiology ,Multidisciplinary ,Thymocytes ,Tissue Scaffolds ,Stem Cells ,Genome Integrity & Repair ,Cell Differentiation ,Thymus ,Cell biology ,Extracellular Matrix ,Lymphatic system ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Regenerative medicine ,Female ,Genetics & Genomics ,Model organisms ,Stromal cell ,T cell ,Science ,Population ,Immunology ,Mice, Nude ,Thymus Gland ,Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,Immune system ,In vivo ,medicine ,Animals ,Humans ,Regeneration ,Progenitor cell ,education ,Computational & Systems Biology ,FOS: Clinical medicine ,Epithelial Cells ,General Chemistry ,Cell Biology ,Tumour Biology ,Rats ,Transplantation ,030104 developmental biology ,Stromal Cells ,Developmental Biology - Abstract
The thymus is a primary lymphoid organ, essential for T cell maturation and selection. There has been long-standing interest in processes underpinning thymus generation and the potential to manipulate it clinically, because alterations of thymus development or function can result in severe immunodeficiency and autoimmunity. Here, we identify epithelial-mesenchymal hybrid cells, capable of long-term expansion in vitro, and able to reconstitute an anatomic phenocopy of the native thymus, when combined with thymic interstitial cells and a natural decellularised extracellular matrix (ECM) obtained by whole thymus perfusion. This anatomical human thymus reconstruction is functional, as judged by its capacity to support mature T cell development in vivo after transplantation into humanised immunodeficient mice. These findings establish a basis for dissecting the cellular and molecular crosstalk between stroma, ECM and thymocytes, and offer practical prospects for treating congenital and acquired immunological diseases., The thymus is essential for T cell maturation and selection, and thymic defects result in severe immune problems. Here the authors identify a thymus cell population that is expandable in vitro, and can repopulate natural thymic matrix to generate tissue that supports mature T cell development in vitro and in vivo.
- Published
- 2021
- Full Text
- View/download PDF
5. The Transcriptional Regulator Prdm1 Is Essential for the Early Development of the Sensory Whisker Follicle and Is Linked to the Beta-Catenin First Dermal Signal
- Author
-
Pierluigi G. Manti, Fabrice Darbellay, Marion Leleu, Aisling Y. Coughlan, Bernard Moret, Julien Cuennet, Frederic Droux, Magali Stoudmann, Gian-Filippo Mancini, Agnès Hautier, Jessica Sordet-Dessimoz, Stephane D. Vincent, Giuseppe Testa, Giulio Cossu, Yann Barrandon, Ecole Polytechnique Fédérale de Lausanne (EPFL), Università degli Studi di Milano = University of Milan (UNIMI), European Institute of Oncology [Milan] (ESMO), Université de Genève = University of Geneva (UNIGE), BioInformatics Competence Center [Lausanne], UNIL-EPFL, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Manchester [Manchester], IRCCS San Raffaele Hospital, Centre Hospitalier Universitaire Vaudois [Lausanne] (CHUV), Duke-National University of Singapore Graduate Medical School, Singapore General Hospital, A*STAR Skin Research Labs, and VINCENT, Stéphane
- Subjects
repressor ,architecture ,leaf ,[SDV]Life Sciences [q-bio] ,blimp-1 ,Medicine (miscellaneous) ,lef1 ,progenitor cells ,terminal differentiation ,organization ,hair-follicles ,General Biochemistry, Genetics and Molecular Biology ,[SDV] Life Sciences [q-bio] ,prdm1 ,non-conserved enhancer ,cortex ,sensory vibrissae ,barrel cortex ,Prdm1 ,Lef1 ,Leaf ,expression ,stem - Abstract
International audience; Prdm1 mutant mice are one of the rare mutant strains that do not develop whisker hair follicles while still displaying a pelage. Here, we show that Prdm1 is expressed at the earliest stage of whisker development in clusters of mesenchymal cells before placode formation. Its conditional knockout in the murine soma leads to the loss of expression of Bmp2, Shh, Bmp4, Krt17, Edar, and Gli1, though leaving the β-catenin-driven first dermal signal intact. Furthermore, we show that Prdm1 expressing cells not only act as a signaling center but also as a multipotent progenitor population contributing to the several lineages of the adult whisker. We confirm by genetic ablation experiments that the absence of macro vibrissae reverberates on the organization of nerve wiring in the mystacial pads and leads to the reorganization of the barrel cortex. We demonstrate that Lef1 acts upstream of Prdm1 and identify a primate-specific deletion of a Lef1 enhancer named Leaf. This loss may have been significant in the evolutionary process, leading to the progressive defunctionalization and disappearance of vibrissae in primates.
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