44 results on '"Pierguidi, L."'
Search Results
2. Responsiveness to warning sensations and anxiety-related psychological traits modulate individual differences in preference for vegetable foods with varied sensory properties
- Author
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Pierguidi, L., Spinelli, S., Prescott, J., Monteleone, E., and Dinnella, C.
- Published
- 2023
- Full Text
- View/download PDF
3. Skin conductance responses to oral stimuli: The role of taste quality and intensity, and personality traits
- Author
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Spinelli, S., Pierguidi, L., Gavazzi, G., Dinnella, C., De Toffoli, A., Prescott, J., and Monteleone, E.
- Published
- 2023
- Full Text
- View/download PDF
4. Chemosensory quality and intensity reflected in implicit affective responses
- Author
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Pierguidi, L., Prescott, J., Spinelli, S., Dinnella, C., De Toffoli, A., White, T.L., and Monteleone, E.
- Published
- 2023
- Full Text
- View/download PDF
5. Erratum to “Skin conductance responses to oral stimuli: The role of taste quality and intensity, and personality traits”. [Food Qual. Prefer. 109 (2023) 104917]
- Author
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Spinelli, S., primary, Pierguidi, L., additional, Gavazzi, G., additional, Dinnella, C., additional, De Toffoli, A., additional, Prescott, J., additional, and Monteleone, E., additional
- Published
- 2024
- Full Text
- View/download PDF
6. Automatic and controlled attentional orienting in the elderly: A dual-process view of the positivity effect
- Author
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Gronchi, G., Righi, S., Pierguidi, L., Giovannelli, F., Murasecco, I., and Viggiano, M.P.
- Published
- 2018
- Full Text
- View/download PDF
7. A magnetization transfer study of mild and advanced Parkinson’s disease
- Author
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Tambasco, N, Belcastro, V, Sarchielli, P, Floridi, P, Pierguidi, L, Menichetti, C, Castrioto, A, Chiarini, P, Parnetti, L, Eusebi, P, Calabresi, P, and Rossi, A
- Published
- 2011
- Full Text
- View/download PDF
8. Individual variation in fungiform papillae density with different sizes and relevant associations with responsiveness to oral stimuli
- Author
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Piochi, M., primary, Pierguidi, L., additional, Torri, L., additional, Spinelli, S., additional, Monteleone, E., additional, Aprea, E., additional, Arena, E., additional, Borgogno, M., additional, Cravero, M.C., additional, Galassi, L., additional, Gatti, E., additional, Lozano, L., additional, Musi, V., additional, Piasentier, E., additional, Valli, E., additional, and Dinnella, C., additional
- Published
- 2019
- Full Text
- View/download PDF
9. Hyperhomocysteinemia recurrence in levodopa-treated Parkinsonʼs disease patients
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Belcastro, V., Pierguidi, L., Castrioto, A., Menichetti, C., Gorgone, G., Ientile, R., Pisani, F., Rossi, A., Calabresi, P., and Tambasco, N.
- Published
- 2010
- Full Text
- View/download PDF
10. Multiple attack study on the available triptans in Italy versus placebo
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Vollono, C., Capuano, A., Mei, D., Ferraro, D., Pierguidi, L., Evangelista, M., and Di Trapani, G.
- Published
- 2005
11. A magnetization transfer study ofmild and advanced Parkinson's disease
- Author
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Tambasco, N., Belcastro, V., Sarchielli, P., Floridi, P., Pierguidi, L., Menichetti, C., Castrioto, A., Chiarini, P., Parnetti, L., Eusebi, P., Calabresi, P., and Rossi, A.
- Published
- 2011
- Full Text
- View/download PDF
12. A magnetization transfer study of mild and advanced Parkinson’s disease
- Author
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Tambasco, N., Belcastro, V., Sarchielli, P., Floridi, P., Pierguidi, L., Menichetti, C., Castrioto, A., Chiarini, P., Parnetti, L., Eusebi, P., Calabresi, P., and Rossi, A.
- Published
- 2010
- Full Text
- View/download PDF
13. Hyperhomocysteinemia recurrence in levodopa-treated Parkinson's disease patients
- Author
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Belcastro, V, Pierguidi, L, Castrioto, Anna, Menichetti, C, Gorgone, G, Ientile, R, Pisani, F, Rossi, Aroldo, Calabresi, Paolo, and Tambasco, Nicola
- Published
- 2010
14. Levetiracetam in newly diagnosed late-onset post-stroke seizures: A prospectiveobservational study
- Author
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Belcastro, V, Costa, C, Galletti, F, Autuori, A, Pierguidi, L, Pisani, Francesco, Calabresi, P, and Parnetti, L.
- Published
- 2008
15. Antiepileptic drugs in migraine prophylaxis: state of the art
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Capuano, A., Catello Vollono, Mei, D., Pierguidi, L., Ferraro, D., and Di Trapani, G.
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Levetiracetam ,Cyclohexanecarboxylic Acids ,Migraine Disorders ,Nipecotic Acids ,Fructose ,Acetates ,Lamotrigine ,Topiramate ,Humans ,Amines ,Tiagabine ,gamma-Aminobutyric Acid ,Clinical Trials as Topic ,Triazines ,Valproic Acid ,Isoxazoles ,Piracetam ,United States ,Primary Prevention ,Zonisamide ,Practice Guidelines as Topic ,Anticonvulsants ,Gabapentin ,Excitatory Amino Acid Antagonists - Abstract
Antiepileptic drugs have proven their efficacy in the prophylactic treatment of migraine. Our study comprises a clinical trial that examines the efficacy of gabapentin and topiramate and a description of the pharmacologic characteristics and the efficacy of tiagabine, lamotrigine, levetiracetam and zonisamide. Antiepileptic drugs have multiple modes of action which can explain their efficacy in reducing neuronal excitability which is proven in epilepsy and postulated in migraine. The relationship between epilepsy and migraine has, in fact, been much debated but never convincingly proven. Antiepileptic drugs could be useful in migraine prophylaxis as some of these have determined a reduction in the monthly frequency and intensity of crises in subjects suffering from migraine with and without aura. These are the aims that have been proposed by the U.S. Headache Consortium Evidence-Based Guidelines. Further double-blind placebo-controlled studies are necessary in order to assess their safety and efficacy.
- Published
- 2004
16. Ophtalmoplegic migraine: a new case-report to suggest an inflammatory condition
- Author
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Pierguidi, L., Sarchielli, Paola, Alberti, A., Baldi, A., Mazzotta, Giovanni, and Capocchi, Giuseppe
- Subjects
ophtalmoplegic migraine - Published
- 2004
17. Adhesion molecules and integrin expression by lymphocytes in the internal jugular blood of migraine patients during attacks
- Author
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Sarchielli, Paola, Alberti, A., Mazzotta, Giovanni, Vaianella, L., Pierguidi, L., Floridi, A., and Gallai, Virgilio
- Subjects
adhesion molecules ,jugular blood ,headache attacks - Published
- 2003
18. Cerebrospinal fluid β-glucocerebrosidase activity is reduced in Dementia with Lewy Bodies
- Author
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Parnetti, L., primary, Balducci, C., additional, Pierguidi, L., additional, De Carlo, C., additional, Peducci, M., additional, D'Amore, C., additional, Padiglioni, C., additional, Mastrocola, S., additional, Persichetti, E., additional, Paciotti, S., additional, Bellomo, G., additional, Tambasco, N., additional, Rossi, A., additional, Beccari, T., additional, and Calabresi, P., additional
- Published
- 2009
- Full Text
- View/download PDF
19. Is retinal assessment useful in epileptic patients with hyperhomocysteinemia?
- Author
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Belcastro, V, primary, Striano, P, additional, Ciampa, C, additional, Pierguidi, L, additional, Napoli, M, additional, Freno, M C, additional, Tenore, R, additional, Striano, S, additional, Pisani, F, additional, and Trombetta, C J, additional
- Published
- 2008
- Full Text
- View/download PDF
20. Is retinal assessment useful in epileptic patients with hyperhomocysteinemia?
- Author
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Belcastro, V., Striano, P., Ciampa, C., Pierguidi, L., Napoli, M., Freno, M. C., Tenore, R., Striano, S., Pisani, F., and Trombetta, C. J.
- Subjects
PEOPLE with epilepsy ,RETINAL blood vessels ,HOMOCYSTEINE ,HIGH performance liquid chromatography ,SULFUR amino acids ,OPHTHALMOLOGY - Abstract
PurposeThe arteriole-to-venule ratio (AVR) is widely used for investigating subclinical cerebral microangiopathy. The possible occurrence of retinal vascular caliber changes was investigated in a population of hyperhomocysteinaemic (plasma total homocysteine (tHcy) >13 μmol/l) adult epileptic patients.MethodsRetinal photographs of cases and controls were evaluated for generalized narrowing of the retinal arterioles, measured as AVR, by graders masked to case–control status using standardized protocols. Plasma total homocysteine (tHcy) levels were assayed by HPLC.ResultsSixty-seven patients (36M/31F, 36.4±7.5 years of age; mean level of tHcy 22.8±11.4 μmol/l), and 75 control subjects (42M/33F, 35.4±8.5 years of age; mean level of tHcy 7.8±2.3 μmol/l) were enroled. No retinal caliber changes were detected in any patient and healthy subject. The analysis of retinal photographs failed to identify any difference in the venular diameters, arteriolar diameters, and AVR measurements between the two groups (P=0.98).Conclusionswe showed that adult hyperhomocysteinaemic epileptic patients do not show any changes in retinal vascular caliber assessed by the measurement of AVR.Eye (2009) 23, 1532–1534; doi:10.1038/eye.2008.326; published online 31 October 2008 [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
21. Combined influence of TAS2R38 genotype and PROP phenotype on the intensity of basic tastes, astringency and pungency in the Italian taste project
- Author
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Paolo Gasparini, Ella Pagliarini, Erminio Monteleone, John F. Prescott, Caterina Dinnella, Beverly J. Tepper, Luisa Torri, Lapo Pierguidi, Flavia Gasperi, Sara Spinelli, Maria Pina Concas, Antonietta Robino, Tullia Gallina Toschi, Robino, A., Concas, M. P., Spinelli, S., Pierguidi, L., Tepper, B. J., Gasparini, P., Prescott, J., Monteleone, E., Gallina Toschi, T., Torri, L., Pagliarini, E., Gasperi, F., Dinnella, C., Robino A., Concas M.P., Spinelli S., Pierguidi L., Tepper B.J., Gasparini P., Prescott J., Monteleone E., Gallina Toschi T., Torri L., Pagliarini E., Gasperi F., and Dinnella C.
- Subjects
Pungency ,Taste ,Nutrition and Dietetics ,Astringency ,Umami ,Biology ,Sweetness ,PROP phenotype ,Recursive partitioning ,TAS2R38 ,Tastes ,Phenotype ,humanities ,Intensity (physics) ,Settore AGR/15 - SCIENZE E TECNOLOGIE ALIMENTARI ,stomatognathic system ,Genotype ,Food Science ,Demography - Abstract
The combined influence of TAS2R38 genotype and PROP phenotype on oral sensations is still to be clarified. The present work investigates their influence on the intensity of basic tastes and somatosensory stimuli (capsaicin, aluminium sulphate), using a large cohort of 1117 individuals. The possible influences of gustin genotype and fungiform papillae density were also assessed. PROP phenotype was mainly associated with TAS2R38 genotype with AVI/AVI individuals reporting the lowest mean bitterness intensity (12.6 ± 1.26), and PAV/AVI individuals rating PROP lower (46.53 ± 0.93) than PAV/PAV individuals (54.14 ± 1.33). However, 25% of AVI/AVI subjects reported PROP bitterness perception higher than ‘moderate’ and small percentages of both PAV/PAV and PAV/AVI responded very little to PROP stimulation. PROP phenotype significantly affected ratings to all the tastant solutions with ST subjects giving the highest ratings and NT the lowest. An unexpected systematic effect of TAS2R38 diplotype on perceived intensity was found, with AVI/AVI individuals rating tastant solution intensity higher than PAV/AVI and PAV/PAV for all the stimuli. Recursive partitioning analysis was used to determine the influence of the explanatory variables (TAS2R38 diplotype, PROP status, age and gender) on intensity for each tastant solution. Regression trees indicated that TAS2R38 genotype is the most important variable for explaining differences in intensity of basic tastes and astringency, when compared to PROP responsiveness, gender, and age. Gender was the primary determinant of heightened perception of pungency. PROP status was the second most influential variable in all the models, with limited influence only on sweetness and umami perception. No significant variations of intensity of taste and somatosensory sensations were found in association to gustin polymorphism or fungiform papillae density. These findings call for a re-examination of the notion that the TAS2R38 gene uniquely controls PROP tasting and for future research devoted to a more in-depth genetic characterization of the AVI/AVI group and its possible associations with other polymorphisms.
- Published
- 2022
22. Phenol-Rich Food Acceptability: The Influence of Variations in Sweetness Optima and Sensory-Liking Patterns
- Author
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Caterina Dinnella, Elena Arena, Lapo Pierguidi, Erminio Monteleone, Isabella Endrizzi, Tullia Gallina Toschi, Cristina Proserpio, Luisa Torri, John F. Prescott, Ada Braghieri, Rossella Di Monaco, Sara Spinelli, Spinelli S., Prescott J., Pierguidi L., Dinnella C., Arena E., Braghieri A., Di Monaco R., Toschi T.G., Endrizzi I., Proserpio C., Torri L., Monteleone E., Spinelli, S., Prescott, J., Pierguidi, L., Dinnella, C., Arena, E., Braghieri, A., Di Monaco, R., Toschi, T. G., Endrizzi, I., Proserpio, C., Torri, L., and Monteleone, E.
- Subjects
0301 basic medicine ,Male ,Sensory-liking pattern ,sensory-liking patterns ,Cluster Analysis ,Food science ,Big Five personality traits ,Personality trait ,media_common ,Nutrition and Dietetics ,digestive, oral, and skin physiology ,food and beverages ,04 agricultural and veterinary sciences ,Sweetness ,Middle Aged ,040401 food science ,Preference ,sweet liking ,Phenol-rich food ,Food, Fortified ,Taste Threshold ,Female ,Psychology ,lcsh:Nutrition. Foods and food supply ,Human ,Personality ,Adult ,Demographics ,Adolescent ,media_common.quotation_subject ,Food Preference ,lcsh:TX341-641 ,Context (language use) ,Sensory system ,Added sugar ,Individual difference ,Article ,Food Preferences ,03 medical and health sciences ,Young Adult ,0404 agricultural biotechnology ,stomatognathic system ,Humans ,PROP ,individual differences ,Aged ,030109 nutrition & dietetics ,Cluster Analysi ,Phenol ,Settore AGR/15 - SCIENZE E TECNOLOGIE ALIMENTARI ,phenol-rich foods ,Propylthiouracil ,Sweetening Agents ,phenol-rich foods, sweet liking, sensory-liking patterns, individual differences, personality traits, PROP ,personality traits ,Sweetening Agent ,Food Science - Abstract
The consumption of phenol-rich foods is limited by their prominent bitterness and astringency. This issue has been addressed by adding sweet tastes, which suppress bitterness, but this is not a complete solution since individuals also differ in their preference for sweetness. In this study, we aimed at identifying groups of consumers differing in sweetness optima and sensory-liking patterns. To this end, increasing concentrations of sucrose were added to a chocolate pudding base. This allowed us to (1) investigate if individual differences in sensory responses are associated with different sweet liking optima in a product context, (2) define the psychological and oro-sensory profile of sweet liker phenotypes derived using a product context, and (3) assess if individuals differing in sweet liking optima differ also in consumption and liking of phenol-rich foods and beverages as a function of their sensory properties (e.g., sweeter vs. more bitter and astringent products). Individuals (1208, 58.4% women, 18–69 years) were characterised for demographics, responsiveness to 6-n-propylthiouracil (PROP), personality traits and attitudes toward foods. Three clusters were identified based on correlations between sensory responses (sweetness, bitterness and astringency) and liking of the samples: liking was positively related to sweetness and negatively to bitterness and astringency in High and Moderate Sweet Likers, and the opposite in Inverted U-Shaped. Differences between clusters were found in age, gender and personality. Furthermore, the Inverted-U Shaped cluster was found to have overall healthier food behaviours and preferences, with higher liking and consumption of phenol-rich vegetables and beverages without added sugar. These findings point out the importance of identifying the individual sensory-liking patterns in order to develop more effective strategies to promote the acceptability of healthy phenol-rich foods.
- Published
- 2021
- Full Text
- View/download PDF
23. Individual variation in fungiform papillae density with different sizes and relevant associations with responsiveness to oral stimuli
- Author
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Lapo Pierguidi, Enrico Valli, Monica Borgogno, L. Galassi, Caterina Dinnella, Edi Piasentier, Maria Carla Cravero, Erminio Monteleone, Eugenio Aprea, V. Musi, Luisa Torri, Sara Spinelli, Maria Piochi, Elena Arena, L. Lozano, Edoardo Gatti, Piochi M., Pierguidi L., Torri L., Spinelli S., Monteleone E., Aprea E., Arena E., Borgogno M., Cravero M.C., Galassi L., Gatti E., Lozano L., Musi V., Piasentier E., Valli E., and Dinnella C.
- Subjects
Taste ,High density ,Biology ,Fungiform papillae pattern ,Automated image analysis ,Basic taste ,Animal science ,Age ,stomatognathic system ,Tongue ,medicine ,Low density ,PROP ,Large diameter ,Lingual papilla ,Pear juice ,Gender ,Basic tastes ,Nutrition and Dietetics ,Automated image analysi ,stomatognathic diseases ,medicine.anatomical_structure ,Settore AGR/15 - SCIENZE E TECNOLOGIE ALIMENTARI ,Food products ,Food Science - Abstract
The association of fungiform papillae (FP) density with responsiveness to oral sensations is controversial. It has been speculated that FP size is a relevant feature for oral responsiveness, thus partially accounting for inconsistencies between FP density and oral responsiveness. This study aims to evaluate factors affecting the number of FP with different diameter sizes (age, gender, PROP status) and to explore the relevant associations with responsiveness to oral sensations in water solutions and food products. Three-hundred-fifty subjects participating in the Italian Taste project were involved, balanced for gender (53% females) and age class (18-30, 35%; 31-45, 35%; 46-60, 30%). The automated image analysis of tongue pictures was used to detect FP and count their frequencies, distinguishing 11 diameter size classes (range from 0.3 to 1.05 mm). Frequencies were converted into FP densities (FP/cm 2 ) for each size. Taste responsiveness in water solutions (sour, bitter, salty, umami, sweet, astringent, pungent) and in three food products with varied intensity of target sensations (pear juice for sourness; bean puree for saltiness; tomato juice for pungency) was measured. Density of FP from all size classes decreased with age. Females showed higher FP density in size of 0.78-0.84 mm diameter, and males in the size of 0.44-0.49 mm. PROP status did not significantly affect the density of FP in any size class. Principal Component Analysis on individual FP density values of the 11 classes allowed identifying four subject groups with different FP patterns: high density and large diameter (HighLarge), high density and small diameter (HighSmall), low density and large diameter (LowLarge), low density and small diameter (LowSmall). FP pattern groups significantly affected oral responsiveness, the highest responsiveness was associated to the uniform pattern characterized by low density and small size FP.
- Published
- 2019
24. Is retinal assessment useful in epileptic patients with hyperhomocysteinemia?
- Author
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R Tenore, Vincenzo Belcastro, M C Freno, S. Striano, Costantino John Trombetta, P Striano, Francesco Pisani, Laura Pierguidi, M. Napoli, C Ciampa, Belcastro, V, Striano, Pasquale, Ciampa, C, Pierguidi, L, Napoli, M, Freno, Mc, Tenore, R, Striano, Salvatore, Pisani, F, and Trombetta, C. J.
- Subjects
Adult ,Male ,Hyperhomocysteinemia ,medicine.medical_specialty ,complications ,Homocysteine ,Population ,Retina ,Central nervous system disease ,Epilepsy ,chemistry.chemical_compound ,Adult, Arterioles ,pathology, Epilepsy ,complications, Female, Humans, Hyperhomocysteinemia ,complications, Male, Middle Aged, Retina ,pathology, Retinal Vessels ,pathology, Venules ,pathology ,Venules ,Ophthalmology ,medicine ,Humans ,education ,Subclinical infection ,education.field_of_study ,business.industry ,Retinal Vessels ,Retinal ,Middle Aged ,medicine.disease ,Surgery ,Arterioles ,medicine.anatomical_structure ,chemistry ,Female ,business - Abstract
PURPOSE: The arteriole-to-venule ratio (AVR) is widely used for investigating subclinical cerebral microangiopathy. The possible occurrence of retinal vascular caliber changes was investigated in a population of hyperhomocysteinaemic (plasma total homocysteine (tHcy) >13 micromol/l) adult epileptic patients. METHODS: Retinal photographs of cases and controls were evaluated for generalized narrowing of the retinal arterioles, measured as AVR, by graders masked to case-control status using standardized protocols. Plasma total homocysteine (tHcy) levels were assayed by HPLC. RESULTS: Sixty-seven patients (36M/31F, 36.4+/-7.5 years of age; mean level of tHcy 22.8+/-11.4 micromol/l), and 75 control subjects (42M/33F, 35.4+/-8.5 years of age; mean level of tHcy 7.8+/-2.3 micromol/l) were enroled. No retinal caliber changes were detected in any patient and healthy subject. The analysis of retinal photographs failed to identify any difference in the venular diameters, arteriolar diameters, and AVR measurements between the two groups (P=0.98). CONCLUSIONS: We showed that adult hyperhomocysteinaemic epileptic patients do not show any changes in retinal vascular caliber assessed by the measurement of AVR.
- Published
- 2009
25. Markers of sensory dynamics in phenols-rich virgin olive oils under optimal storage conditions.
- Author
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Pierguidi L, Cecchi L, Dinnella C, Zanoni B, Spinelli S, Migliorini M, and Monteleone E
- Subjects
- Humans, Taste, Chromatography, High Pressure Liquid, Gas Chromatography-Mass Spectrometry, Male, Female, Adult, Biomarkers analysis, Iridoids analysis, Olive Oil chemistry, Phenols analysis, Food Storage methods, Volatile Organic Compounds analysis
- Abstract
Early changes in sensory quality of phenols-rich virgin olive oil (VOO) and their relationship with the chemical changes are less studied in the literature. Therefore, the objective of this study was to propose a predictive model of dynamics of sensory changes based on specific chemical markers. The evolution of the sensory quality of phenol-rich VOOs from Tuscan cultivars stored under optimal storage conditions (i.e., absence of light, no O
2 exposure, low temperature) was investigated using a multi-step methodological approach combining sensory (official sensory analysis (so-called Panel Test), Descriptive Analysis and Temporal Dominance of Sensation) and chemical measurements. The sensory map from descriptive data was related to the phenolic and volatile profiles, measured using HPLC-DAD and HS-SPME-GC-MS, respectively. A predictive model of the sensory changes over storage based on chemical compounds was developed. Results showed that very early changes involving phenolic and volatile compounds profiles occur in VOOs stored under optimal storage conditions, which turn in changes in sensory properties evaluated by the official panel test, the descriptive analysis and the temporal dominance of sensation. Furthermore, a chemical marker of sensory dynamics of oils during storage was identified as the ratio between two groups of secoiridoids. The proposed model, supported by the mentioned chemical marker, has the potential of improving the control of sensory changes in phenols-rich virgin olive oils during storage in optimal conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
26. Exploring the association between oral tactile sensitivity measures and phenotypic markers of oral responsiveness.
- Author
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Mani E, Ford R, Pierguidi L, Spinelli S, Ramsey I, Monteleone E, and Dinnella C
- Subjects
- Biomarkers, Female, Humans, Male, Taste Perception, Tongue, Taste Buds, Touch Perception
- Abstract
This study investigated the individual variability in oral tactile sensitivity considering touch, by means of Von Frey Hair monofilaments (VFH) and spatial resolution, using the grating orientation test (GOT). The relationships of the two measures with 6-n-propylthiouracil (PROP) responsiveness and fungiform papillae density and size were investigated. One hundred and forty-four subjects (48.6% women, aged 18-30) participated in the study. VFH and GOT thresholds were assessed by three-down/one-up staircase method. Responsiveness to 3.2 mM PROP was assessed on the general Labeled Magnitude Scale. Fungiform papillae density (FPD) and size were determined from automated counting. VFH thresholds appeared unsuitable to reveal individual variation in responsiveness to point-pressure on the tongue. The frequency of GOT thresholds approximated a normal distribution and covered the whole range of variation, thus indicating an ability to measure individual variation in oral tactile sensitivity. No significant linear correlations were found between any of the oral tactile sensitivity measures and PROP responsiveness, FPD total and size class. VFH and GOT thresholds were not significantly associated. Agglomerative hierarchical clustering was used to classify participants for their PROP responsiveness, total FPD and GOT threshold. Three clusters were identified, C1 (n = 67), Cl2 (n = 42), and Cl3 (n = 35), differing for PROP responsiveness and FPD only. Results encourage future studies to explore association between GOT and both perception and preference for different food texture. Furthermore, deeper investigation of individual variability in sensitivity to different types of oral tactile stimuli would be helpful to capture differences in tactile sensitivity among the most sensitive individuals., (© 2022 Wiley Periodicals LLC.)
- Published
- 2022
- Full Text
- View/download PDF
27. Remote testing: Sensory test during Covid-19 pandemic and beyond.
- Author
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Dinnella C, Pierguidi L, Spinelli S, Borgogno M, Gallina Toschi T, Predieri S, Lavezzi G, Trapani F, Tura M, Magli M, Bendini A, and Monteleone E
- Abstract
Restrictions adopted by many countries in 2020 due to Covid-19 pandemic had severe consequences on the management of sensory and consumer testing that strengthened the tendency to move data collection out of the laboratory. Remote sensory testing, organized at the assessor's home or workplace and carried out under the live online supervision of the panel leader, represents a trade-off between adequate control and the convenience of conducting testing out of the lab. The Italian Sensory Science Society developed the "Remote sensory testing" research project aimed at testing the effectiveness and validity of the sensory tests conducted remotely through a comparison with evaluations in a classical laboratory setting. Guidelines were developed to assist panel leaders in setting up and controlling the evaluation sessions in remote testing conditions. Different methods were considered: triangle and tetrad tests, Descriptive Analysis and Temporal Dominance of Sensations tests, all of which involved trained panels, and Check-All-That-Apply and hedonic tests with consumers. Remote sensory testing provided similar results to the lab testing in all the cases, with the exception of the tetrad test run at work. Findings suggest that remote sensory testing, if conducted in strict compliance with specifically developed sensory protocols, is a promising alternative to laboratory tests that can be applied with both trained assessors and consumers even beyond the global pandemic., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
28. Phenol-Rich Food Acceptability: The Influence of Variations in Sweetness Optima and Sensory-Liking Patterns.
- Author
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Spinelli S, Prescott J, Pierguidi L, Dinnella C, Arena E, Braghieri A, Di Monaco R, Gallina Toschi T, Endrizzi I, Proserpio C, Torri L, and Monteleone E
- Subjects
- Adolescent, Adult, Aged, Cluster Analysis, Female, Humans, Male, Middle Aged, Personality, Propylthiouracil, Young Adult, Food Preferences psychology, Food, Fortified analysis, Phenol, Sweetening Agents analysis, Taste Threshold drug effects
- Abstract
The consumption of phenol-rich foods is limited by their prominent bitterness and astringency. This issue has been addressed by adding sweet tastes, which suppress bitterness, but this is not a complete solution since individuals also differ in their preference for sweetness. In this study, we aimed at identifying groups of consumers differing in sweetness optima and sensory-liking patterns. To this end, increasing concentrations of sucrose were added to a chocolate pudding base. This allowed us to (1) investigate if individual differences in sensory responses are associated with different sweet liking optima in a product context, (2) define the psychological and oro-sensory profile of sweet liker phenotypes derived using a product context, and (3) assess if individuals differing in sweet liking optima differ also in consumption and liking of phenol-rich foods and beverages as a function of their sensory properties (e.g., sweeter vs. more bitter and astringent products). Individuals (1208; 58.4% women, 18-69 years) were characterised for demographics, responsiveness to 6-n-propylthiouracil (PROP), personality traits and attitudes toward foods. Three clusters were identified based on correlations between sensory responses (sweetness, bitterness and astringency) and liking of the samples: liking was positively related to sweetness and negatively to bitterness and astringency in High and Moderate Sweet Likers, and the opposite in Inverted U-Shaped. Differences between clusters were found in age, gender and personality. Furthermore, the Inverted-U Shaped cluster was found to have overall healthier food behaviours and preferences, with higher liking and consumption of phenol-rich vegetables and beverages without added sugar. These findings point out the importance of identifying the individual sensory-liking patterns in order to develop more effective strategies to promote the acceptability of healthy phenol-rich foods.
- Published
- 2021
- Full Text
- View/download PDF
29. The Meaning of Emoji to Describe Food Experiences in Pre-Adolescents.
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Sick J, Monteleone E, Pierguidi L, Ares G, and Spinelli S
- Abstract
Ongoing research has shown that emoji can be used by children to discriminate food products, but it is unclear if they express emotions and how they are linked to emotional words. Little is known about how children interpret emoji in terms of their emotional meaning in the context of food. This study aimed at investigating the emotional meaning of emoji used to describe food experiences in 9-13-year-old pre-adolescents and to measure related age and gender differences. The meaning of 46 emoji used to describe food experience was explored by: mapping emoji according to similarities and differences in their emotional meaning using the projective mapping technique, and linking emoji with emotion words using a check-all-that-apply (CATA) format. The two tasks gave consistent results and showed that emoji were discriminated along the valence (positive vs. negative) and power (dominant vs. submissive) dimension, and to a lower extent along the arousal dimension (high vs. low activation). In general, negative emoji had more distinct meanings than positive emoji in both studies, but differences in nuances of meaning were found also among positive emoji. Girls and older pre-adolescents (12-13 years old (y.o.)) discriminated positive emoji slightly better than boys and younger pre-adolescents (9-11 y.o.). This suggests that girls and older pre-adolescents may be higher in emotional granularity (the ability to experience and discriminate emotions), particularly of positive emotions. The results of the present work can be used for the development of an emoji-based tool to measure emotions elicited by foods in pre-adolescents.
- Published
- 2020
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30. Validation of a low-cost EEG device in detecting neural correlates of social conformity.
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Pierguidi L, Guazzini A, Imbimbo E, Righi S, Sorelli M, and Bocchi L
- Subjects
- Event-Related Potentials, P300, Evoked Potentials, Humans, Social Conformity, Brain-Computer Interfaces, Electroencephalography instrumentation
- Abstract
The study of conformity from a neurobiological point of view has interested many authors: among them, Shestakova and colleagues (2013) have showed how conformity can be assessed through the analysis of event related potentials (ERPs). More specifically, the P300 component of the ERP was shown to be sensitive to the behavioral adjustment that an individual makes when not agreeing with the majority of a group. Starting from these contributions, in the present study, the famous experiment of Solomon Asch [1] was replicated online. The experiment was conducted on a sample of university students, using an innovative and low-cost tool capable of recording the brain signal (a wireless headset equipped with fourteen electrodes, called Emotiv EPOC). The present research aims to demonstrate how cheap and little sensitive tools enable the detection of ERP components that characterize social conformity in an ecological context.
- Published
- 2019
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31. Individual Variation in PROP Status, Fungiform Papillae Density, and Responsiveness to Taste Stimuli in a Large Population Sample.
- Author
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Dinnella C, Monteleone E, Piochi M, Spinelli S, Prescott J, Pierguidi L, Gasperi F, Laureati M, Pagliarini E, Predieri S, Torri L, Barbieri S, Valli E, Bianchi P, Braghieri A, Caro AD, Di Monaco R, Favotto S, and Moneta E
- Subjects
- Adolescent, Adult, Female, Food Preferences, Humans, Male, Middle Aged, Taste Buds physiology, Young Adult, Propylthiouracil pharmacology, Taste Buds drug effects, Taste Perception physiology
- Abstract
Despite considerable research investigating the role of 6-n-propylthiouracil bitterness perception and variation of fungiform papillae density in food perception, this relationship remains controversial as well as the association between the 2 phenotypes. Data from 1119 subjects (38.6% male; 18-60 years) enrolled in the Italian Taste project were analyzed. Responsiveness to the bitterness of 6-n-propylthiouracil was assessed on the general Labeled Magnitude Scale. Fungiform papillae density was determined from manual counting on digital images of the tongue. Solutions of tastes, astringent, and pungent sensations were prepared to be moderate/strong on a general Labeled Magnitude Scale. Four foods had tastants added to produce 4 variations in target sensations from weak to strong (pear juice: citric acid, sourness; chocolate pudding: sucrose, sweetness; bean purée: sodium chloride, saltiness; and tomato juice: capsaicin, pungency). Women gave ratings to 6-n-propylthiouracil and showed fungiform papillae density that was significantly higher than men. Both phenotype markers significantly decreased with age. No significant correlations were found between 6-n-propylthiouracil ratings and fungiform papillae density. Fungiform papillae density variation does not affect perceived intensity of solutions. Responsiveness to 6-n-propylthiouracil positively correlated to perceived intensity of most stimuli in solution. A significant effect of fungiform papillae density on perceived intensity of target sensation in foods was found in a few cases. Responsiveness to 6-n-propylthiouracil positively affected all taste intensities in subjects with low fungiform papillae density whereas there were no significant effects of 6-n-propylthiouracil in those with high fungiform papillae density. These data highlight a complex interplay between 6-n-propylthiouracil status and fungiform papillae density and the need of a critical reconsideration of their role in food perception and acceptability.
- Published
- 2018
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32. Emotional contexts modulate intentional memory suppression of neutral faces: Insights from ERPs.
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Pierguidi L, Righi S, Gronchi G, Marzi T, Caharel S, Giovannelli F, and Viggiano MP
- Subjects
- Adult, Electroencephalography, Facial Recognition physiology, Female, Humans, Intention, Male, Recognition, Psychology physiology, Young Adult, Affect physiology, Attention physiology, Evoked Potentials physiology, Mental Recall physiology, Pattern Recognition, Visual physiology
- Abstract
The main goal of present work is to gain new insight into the temporal dynamics underlying the voluntary memory control for neutral faces associated with neutral, positive and negative contexts. A directed forgetting (DF) procedure was used during the recording of EEG to answer the question whether is it possible to forget a face that has been encoded within a particular emotional context. A face-scene phase in which a neutral face was showed in a neutral or emotional scene (positive, negative) was followed by the voluntary memory cue (cue phase) indicating whether the face had to-be remember or to-be-forgotten (TBR and TBF). Memory for faces was then assessed with an old/new recognition task. Behaviorally, we found that it is harder to suppress faces-in-positive-scenes compared to faces-in-negative and neutral-scenes. The temporal information obtained by the ERPs showed: 1) during the face-scene phase, the Late Positive Potential (LPP), which indexes motivated emotional attention, was larger for faces-in-negative-scenes compared to faces-in-neutral-scenes. 2) Remarkably, during the cue phase, ERPs were significantly modulated by the emotional contexts. Faces-in-neutral scenes showed an ERP pattern that has been typically associated to DF effect whereas faces-in-positive-scenes elicited the reverse ERP pattern. Faces-in-negative scenes did not show differences in the DF-related neural activities but larger N1 amplitude for TBF vs. TBR faces may index early attentional deployment. These results support the hypothesis that the pleasantness or unpleasantness of the contexts (through attentional broadening and narrowing mechanisms, respectively) may modulate the effectiveness of intentional memory suppression for neutral information., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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33. Intravenous lacosamide as treatment option in post-stroke non convulsive status epilepticus in the elderly: a proof-of-concept, observational study.
- Author
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Belcastro V, Vidale S, Pierguidi L, Sironi L, Tancredi L, Striano P, Taborelli A, and Arnaboldi M
- Subjects
- Acetamides administration & dosage, Aged, Aged, 80 and over, Anticonvulsants administration & dosage, Female, Humans, Lacosamide, Male, Status Epilepticus etiology, Treatment Outcome, Acetamides therapeutic use, Anticonvulsants therapeutic use, Status Epilepticus drug therapy, Stroke complications
- Abstract
Purpose: To evaluate the efficacy and safety of intravenously administered lacosamide (iv LCM) in post-stroke non convulsive status epilepticus (NCSE) in elderly patients., Methods: We enrolled 16 patients (7 M/9 F; 77 ± 7 years of age) with NCSE. iv LCM was used in all the patients as initial treatment (i.e. patients were directly started on LCM) at a loading dose of 400 mg over 30 min, followed by a mean maintenance dose of 400 mg per day. iv LCM was considered as effective in patients who experience no NCSE for 24 h following treatment, as evaluated by EEG recording and clinical observation., Results: LCM was effective in treating NCSE in eight of the sixteen patients in whom epileptic activity disappeared (7/8) or was significantly reduced (1/8) within 45-60 min after administration. None of these patients relapsed in the following 24 h. No adverse events were observed. A partial anterior circulation syndrome (PACS) was present in 10 patients while a total anterior circulation syndrome (TACS) in six., Conclusions: This pilot study suggests that LCM exhibits safety and efficacy profiles which make it an optimal candidate as a first-choice drug against post-stroke NCSE in elderly patients. A prospective comparative trial is needed to confirm these preliminary data., (Copyright © 2013 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2013
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34. Recurrent hypothermia with hyperhidrosis in two siblings: familial Shapiro syndrome variant.
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Belcastro V, Striano P, Pierguidi L, Arnaboldi M, and Tambasco N
- Subjects
- Brain pathology, Child, Female, Humans, Magnetic Resonance Imaging, Male, Recurrence, Siblings, Young Adult, Agenesis of Corpus Callosum pathology, Hyperhidrosis pathology, Hypothermia pathology
- Published
- 2012
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35. Levetiracetam in brain ischemia: clinical implications in neuroprotection and prevention of post-stroke epilepsy.
- Author
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Belcastro V, Pierguidi L, and Tambasco N
- Subjects
- Animals, Brain Ischemia drug therapy, Clinical Trials as Topic, Disease Models, Animal, Epilepsy etiology, Humans, Levetiracetam, Piracetam therapeutic use, Anticonvulsants therapeutic use, Epilepsy prevention & control, Neuroprotective Agents therapeutic use, Piracetam analogs & derivatives, Stroke complications
- Abstract
Several new antiepileptic drugs (AEDs) have been introduced for clinical use recently. These new AEDs, like the classic AEDs, target multiple cellular sites both pre- and postsynaptically. The use of AEDs as a possible neuroprotective strategy in brain ischemia is receiving increasing attention and the antiepileptic drug levetiracetam, a 2S-(2-oxo-1-pyrrolidiny1) butanamide, belonging to the pyrrolidone family, could have a crucial role in regulation of epileptogenesis and neuroprotection. Recent observations suggest that levetiracetam is both safe and effective against post-stroke seizures. In this review, the potential neuroprotective role in brain ischemia and the therapeutic implications of levetiracetam in post-stroke epilepsy are discussed., (Copyright © 2010 The Japanese Society of Child Neurology. Published by Elsevier B.V. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
36. Decreased contralateral putamen [I]FP-CIT SPECT uptake in hyperglycemic hemichorea-hemiballismus.
- Author
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Belcastro V, Pierguidi L, Tambasco N, Sironi L, Sacco L, Corso A, Taborelli A, and Arnaboldi M
- Subjects
- Aged, 80 and over, Dyskinesias etiology, Humans, Hyperglycemia complications, Male, Dyskinesias diagnostic imaging, Dyskinesias pathology, Functional Laterality physiology, Putamen diagnostic imaging, Tomography, Emission-Computed, Single-Photon, Tropanes
- Published
- 2011
- Full Text
- View/download PDF
37. Ictal epileptic headache mimicking status migrainosus: EEG and DWI-MRI findings.
- Author
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Belcastro V, Striano P, Pierguidi L, Calabresi P, and Tambasco N
- Subjects
- Anticonvulsants therapeutic use, Diffusion Magnetic Resonance Imaging, Electroencephalography, Epilepsy drug therapy, Female, Headache drug therapy, Humans, Lorazepam therapeutic use, Magnetic Resonance Imaging, Young Adult, Epilepsy complications, Headache etiology, Migraine Disorders diagnosis, Seizures complications
- Abstract
We report a case of a patient with status migrainosus unresponsive to analgesic therapy in whom electroencephalographic recording revealed an epileptic origin. Intravenous administration of lorazepam induced the prompt resolution of the symptoms., (© 2010 American Headache Society.)
- Published
- 2011
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- View/download PDF
38. Cerebrospinal fluid beta-glucocerebrosidase activity is reduced in Dementia with Lewy Bodies.
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Parnetti L, Balducci C, Pierguidi L, De Carlo C, Peducci M, D'Amore C, Padiglioni C, Mastrocola S, Persichetti E, Paciotti S, Bellomo G, Tambasco N, Rossi A, Beccari T, and Calabresi P
- Subjects
- Aged, Alzheimer Disease cerebrospinal fluid, Alzheimer Disease enzymology, Dementia cerebrospinal fluid, Dementia enzymology, Female, Humans, Male, Middle Aged, alpha-Mannosidase cerebrospinal fluid, beta-Galactosidase cerebrospinal fluid, beta-Mannosidase cerebrospinal fluid, beta-N-Acetylhexosaminidases cerebrospinal fluid, Glucosylceramidase cerebrospinal fluid, Lewy Body Disease cerebrospinal fluid, Lewy Body Disease enzymology
- Abstract
The autophagy-lysosomal degradation pathway plays a role in the onset and progression of neurodegenerative diseases. Clinical and genetic studies indicate that mutations of beta-glucocerebrosidase represent genetic risk factors for synucleinopathies, including Parkinson's Disease (PD) and Dementia with Lewy Bodies (DLB). We recently found a decreased activity of lysosomal hydrolases, namely beta-glucocerebrosidase, in cerebrospinal fluid of PD patients. We have thus measured the activity of these enzymes - alpha-mannosidase (EC 3.2.1.24), beta-mannosidase (EC 3.2.1.25), beta-glucocerebrosidase (EC 3.2.1.45), beta-galactosidase (EC 3.2.1.23) and beta-hexosaminidase (EC 3.2.1.52) - in cerebrospinal fluid of patients suffering from DLB, Alzheimer's Disease (AD), Fronto-Temporal Dementia (FTD) and controls. Alpha-mannosidase activity showed a marked decrease across all the pathological groups as compared to controls. Conversely, beta-glucocerebrosidase activity was selectively reduced in DLB, further suggesting that this enzyme might specifically be impaired in synucleinopathies.
- Published
- 2009
- Full Text
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39. Levetiracetam in newly diagnosed late-onset post-stroke seizures: a prospective observational study.
- Author
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Belcastro V, Costa C, Galletti F, Autuori A, Pierguidi L, Pisani F, Calabresi P, and Parnetti L
- Subjects
- Aged, Aged, 80 and over, Aggression drug effects, Anticonvulsants administration & dosage, Anticonvulsants adverse effects, Cardiovascular Agents therapeutic use, Drug Interactions, Epilepsy etiology, Female, Fibrinolytic Agents therapeutic use, Gait Ataxia chemically induced, Humans, Levetiracetam, Male, Middle Aged, Piracetam administration & dosage, Piracetam adverse effects, Piracetam therapeutic use, Sleep Stages drug effects, Anticonvulsants therapeutic use, Brain Ischemia complications, Epilepsy drug therapy, Piracetam analogs & derivatives
- Abstract
Levetiracetam (LEV) monotherapy was investigated in 35 patients (pts) (16M/19F, 71.9+/-7.3 years of age) with late-onset post-stroke seizures (i.e. seizures occurring at least 2 weeks after an ischemic stroke) in a prospective open-label study. Overall, 27 pts (77.1%) achieved a condition of seizure freedom (defined as 1 year without seizures): 19 (54.3%) at a daily LEV dose of 1000mg, 7 (20.0%) at 1500mg, 1 (2.8%) at 2000mg. Four pts (11.4%) discontinued the drug because of intolerable side effects (drowsiness associated to gait disturbance in 1 pt, and aggressive behaviour in the remaining 3 pts); 3 pts were unresponsive at a dose of 3000mg, and 1 pt was lost at follow-up. These observations suggest that LEV exhibits safety and efficacy profiles which make it an optimal candidate as a first-choice drug against post-stroke seizures.
- Published
- 2008
- Full Text
- View/download PDF
40. Cerebrospinal fluid biomarkers in Parkinson's disease with dementia and dementia with Lewy bodies.
- Author
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Parnetti L, Tiraboschi P, Lanari A, Peducci M, Padiglioni C, D'Amore C, Pierguidi L, Tambasco N, Rossi A, and Calabresi P
- Subjects
- Aged, Alzheimer Disease cerebrospinal fluid, Analysis of Variance, Biomarkers cerebrospinal fluid, Case-Control Studies, Dementia complications, Female, Humans, Lewy Body Disease complications, Male, Middle Aged, Neuropsychological Tests, Parkinson Disease complications, Amyloid beta-Peptides cerebrospinal fluid, Dementia cerebrospinal fluid, Lewy Body Disease cerebrospinal fluid, Parkinson Disease cerebrospinal fluid, Peptide Fragments cerebrospinal fluid, tau Proteins cerebrospinal fluid
- Abstract
Background: Clinical criteria for differentiating Parkinson's disease (PD) with dementia (PDD) from dementia with Lewy bodies (DLB) are unsatisfactory. Their existence as distinct clinicopathologic entities is still debated, although the burden of Alzheimer's disease (AD) pathology seems higher in DLB. Thus, analysis of cerebrospinal fluid (CSF) biomarkers (beta-amyloid(1-42) [Abeta42], total tau, and hyperphosphorylated tau [p-tau]) in living subjects might provide significant pathophysiological information on these diseases., Methods: Cerebrospinal fluid biomarkers were measured in DLB (n = 19), PDD (n = 18), and AD (n = 23) subjects matched for age, sex, and dementia severity, as well as in PD (n = 20) and normal control subjects (n = 20)., Results: DLB showed the lowest mean CSF Abeta42 levels, with a negative association to dementia duration (rho = -.42, p = .07). In DLB patients, mean CSF total tau levels were significantly lower than in AD patients (508 +/- 387 vs. 960 +/- 619, respectively) but twofold to threefold higher than in PDD (286 +/- 184), PD (160 +/- 64), or normal control subjects (177 +/- 76), with a positive association to dementia severity (Mini-Mental State Examination: rho = -.54, p = .02; Milan Overall Dementia Assessment: rho = -.66, p = .002). PDD patients had mean CSF Abeta42 and total tau levels similar to those seen in PD patients. Hyperphosphorylated tau was significantly increased in the AD group only., Conclusions: Cerebrospinal fluid Abeta42 and total tau have a different behavior in DLB and PDD, being related to duration and severity of dementia in DLB alone. Hyperphosphorylated tau is not significantly altered in these conditions.
- Published
- 2008
- Full Text
- View/download PDF
41. Lysosomal hydrolases in cerebrospinal fluid from subjects with Parkinson's disease.
- Author
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Balducci C, Pierguidi L, Persichetti E, Parnetti L, Sbaragli M, Tassi C, Orlacchio A, Calabresi P, Beccari T, and Rossi A
- Subjects
- Aged, Chromatography, Ion Exchange methods, Female, Humans, Male, Middle Aged, Statistics, Nonparametric, Hyaluronoglucosaminidase cerebrospinal fluid, Parkinson Disease cerebrospinal fluid
- Abstract
Recent studies have shown a genetic association between glucocerebrosidase deficiencies and Parkinson's disease (PD). To further explore this issue the activity of beta-glucocerebrosidase and the activities of other lysosomal enzymes, alpha-mannosidase, beta-mannosidase, beta-hexosaminidase, and beta-galactosidase have been evaluated in the cerebrospinal fluid (CSF) of PD patients. The activities of alpha-mannosidase, beta-mannosidase, beta-glucocerebrosidase, and beta-hexosaminidase were substantially decreased in the CSF of PD patients, while levels of beta-galactosidase were essentially identical to controls. This study indicates that in PD several lysosomal hydrolases have decreased activities, further supporting a possible link between pathophysiological mechanisms underlying PD and lysosomal hydrolases., (Copyright 2007 Movement Disorder Society)
- Published
- 2007
- Full Text
- View/download PDF
42. Proinflammatory cytokines, adhesion molecules, and lymphocyte integrin expression in the internal jugular blood of migraine patients without aura assessed ictally.
- Author
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Sarchielli P, Alberti A, Baldi A, Coppola F, Rossi C, Pierguidi L, Floridi A, and Calabresi P
- Subjects
- Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, Integrin alpha4beta1 blood, Interleukin-1 blood, Interleukin-4 blood, Interleukin-6 blood, Jugular Veins, Lymphocyte Function-Associated Antigen-1 blood, Lymphocytes immunology, Time Factors, Tumor Necrosis Factor-alpha analysis, Cytokines blood, Integrins blood, Intercellular Adhesion Molecule-1 blood, Lymphocytes metabolism, Migraine without Aura blood, Vascular Cell Adhesion Molecule-1 blood
- Abstract
Objective: The aim of the present research was to verify the levels of the soluble adhesion molecules sL- and sE-selectins, intercellular adhesion molecule (sICAM)-1, and vascular cell adhesion molecule-1 in serial samples of internal jugular venous blood taken from migraine patients without aura (MWoA) during attacks. The expression of leukocyte function antigen (LFA)-1 and very late activation antigen (VLA)-4 was also assessed on lymphocytes obtained from jugular venous blood. Levels of certain proinflammatory cytokines (tumor necrosis factor-alpha[TNF-alpha], interleukin-1beta[IL-1beta], IL-4, and IL-6) were also determined and correlated with those of adhesion molecules., Patients and Methods: Seven MWoA patients were admitted in the hospital during attacks and blood samples were taken immediately after catheter insertion, at 1, 2, and 4 hours after attack onset, and within 2 hours after its termination. The levels of adhesion molecules and cytokines were measured with ELISA method. The expression of LFA-1 and VLA-4 was assessed by flow cytometry., Results: A parallel transient increase of sICAM-1, TNF-alpha, and IL-6 was observed in the first 2 hours after attack onset compared with the time of catheter insertion (P < .0001, <.001, and <.003, respectively). The proportion of CD4+ and CD8+ T-cells expressing high levels of LFA-1 showed instead a progressive down-regulation with significantly lower percentages at 2 and 4 hours after attack onset (P < .01 and <.022, respectively). No variation in the percentage of VLA-4 expressing cells was observed at any time of the study., Conclusions: The transient increase in sICAM-1 and TNF-alpha found in the internal jugular blood of MWoA patients assessed ictally can be induced by sensory neuropeptides released from activated trigeminal endings. The progressive decrease in sICAM-1 levels during attacks and the down-regulation of LFA-1 expression by lymphocytes could antagonize their transvascular migration, supporting the hypothesis of sterile inflammation in the dura mater during migraine attacks.
- Published
- 2006
- Full Text
- View/download PDF
43. Chemokine levels in the jugular venous blood of migraine without aura patients during attacks.
- Author
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Sarchielli P, Alberti A, Vaianella L, Pierguidi L, Floridi A, Mazzotta G, Floridi A, and Gallai V
- Subjects
- Adult, Chemokine CCL5 blood, Female, Humans, Interleukin-8 blood, Jugular Veins, Male, Calcitonin Gene-Related Peptide blood, Chemokines blood, Cyclic AMP blood, Migraine without Aura blood
- Abstract
Objective: To investigate changes in the levels of calcitonin gene-related peptide and its intracellular messenger cyclic adenosine monophosphate in serial samples of internal jugular blood taken from migraine patients without aura assessed during attacks, and to assess their relationship with the levels of IL-8, MCP-1, and RANTES in the same samples., Background: Calcitonin gene-related peptide, the marker of trigeminovascular activation, is released in both the internal and external jugular venous blood of migraine patients during attacks. Experimental evidence demonstrated that when released from C-type sensory neurons in inflammatory pain models, it differentially induced expression of neutrophil chemotactic chemokine IL-8, but not monocyte chemotactic chemokine MCP-1 or lymphocyte chemotactic chemokine RANTES. These chemokines were never investigated in migraine., Design/methods: Eight migraine without aura patients were admitted to the hospital during the attacks. Internal jugular venous blood samples were taken immediately after catheter insertion, at the 1st, 2nd, and 4th hours after attack onset, and within 2 hours from its cessation. The levels of the sensory neuropeptide calcitonin gene-related peptide and the messenger cyclic adenosine monophosphate were measured by RIA method, and those of IL-8, MCP-1, and RANTES were measured by ELISA method., Results: Higher calcitonin gene-related peptide levels were found in the internal jugular venous blood of migraine without aura patients compared with the time of catheter insertion (ANOVA: P<.0001) with a peak at the first hour (52.6+/-9.2 ng/mL). A transient increase in IL-8 was observed at the 2nd and 4th hours (P<.01 and P<.002, respectively), whereas no changes in the levels of MCP-1 and RANTES were found at any time of the study. The increase in IL-8 was accompanied by a parallel increase in cyclic adenosine monophosphate., Conclusions: The present study confirms previous findings of an increase in calcitonin gene-related peptide in internal jugular venous blood of migraine without aura patients during attacks. The transient increase in the levels of IL-8 concurs with the results of recent experimental research showing a calcitonin gene-related peptide-induced activation of IL-8 gene expression, but not RANTES and MCP-1, via the transcriptional factor AP-2, which mediates transduction in response to cyclic adenosine monophosphate. Although IL-8 is transiently increased during migraine attacks, an accumulation of leukocytes secondary to neurogenic inflammation is unlikely, as it is for other inflammatory events, because they are self limiting. Other events, including nitric oxide production, may contribute to counteract meningeal transvascular leukocyte migration during migraine attacks, as suggested by the model of sterile inflammation.
- Published
- 2004
- Full Text
- View/download PDF
44. [Acute experimental hepatic insufficiency caused by total ischemia of the liver: effect of peritoneal dialysis].
- Author
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Cassina I, Reggiori G, Tagliatesta GL, Saba V, Addis F, Mortellaro C, Rusca A, Ferretti A, Pierguidi L, Valenti G, Miradoli R, and Quarto di Palo F
- Subjects
- Alanine Transaminase blood, Ammonia blood, Animals, Aspartate Aminotransferases blood, Creatinine blood, Dogs, Electroencephalography, Female, Male, Ischemia therapy, Liver blood supply, Liver Diseases therapy, Peritoneal Dialysis
- Published
- 1979
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