474 results on '"Pieniazek, P."'
Search Results
2. Microplastic Analysis in Soil Using Ultra-High-Resolution UV–Vis–NIR Spectroscopy and Chemometric Modeling
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Lori Shelton Pieniazek, Michael L. McKinney, Jake A. Carr, and Lei Shen
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vermiculite ,plastic ,spectral ,quantitative ,polymer ,regression ,Biology (General) ,QH301-705.5 ,Microbiology ,QR1-502 ,Biochemistry ,QD415-436 - Abstract
The study of microplastics (MPs) in soils is impeded by similarities between plastic and non-plastic particles and the misidentification of MP by current analytical methods such as visual microscopic examination. Soil MPs pose serious ecological and public health risks because of their abundance, persistence, and ubiquity. Thus, reliable identification methods are badly needed for scientific study. One possible solution is UV–Vis–NIR spectroscopy, which has the ability to rapidly identify and quantify concentrations of soil microplastics. In this study, a full-range, field portable spectrometer (350–2500 nm) with ultra-high spectral resolution (1.5 nm, 3.0 nm, and 3.8 nm) identified three types of common plastics: low-density polyethylene (LDPE), polyvinyl chloride (PVC), and polypropylene (PP). Three sets of artificially MP-treated vermiculite soil samples were prepared for model prediction testing and validation: 150 samples for model calibration and 50 samples for model validation. A partial least square regression model using the spectral signatures for quantification of soil and MP mixtures was built with all three plastic polymers. Prediction R2 values of all three polymers showed promising results: polypropylene R2 = 0.943, polyvinyl chloride R2 = 0.983, and polyethylene R2 = 0.957. Our study supports previous work showing that combining ultra-high-resolution UV–Vis–NIR spectrometry with quantitative modeling can improve the accuracy and speed of MP identification and quantification in soil.
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- 2024
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3. Imaging modality-dependent carotid stenosis severity variations against intravascular ultrasound as a reference: Carotid Artery intravasculaRUltrasound Study (CARUS)
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Tekieli, Lukasz, Kablak-Ziembicka, Anna, Dabrowski, Wladyslaw, Dzierwa, Karolina, Moczulski, Zbigniew, Urbanczyk-Zawadzka, Malgorzata, Mazurek, Adam, Stefaniak, Justyna, Paluszek, Piotr, Krupinski, Maciej, Przewlocki, Tadeusz, Pieniazek, Piotr, and Musialek, Piotr
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- 2023
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4. Indoxyl sulfate induces apoptosis in mononuclear blood cells via mitochondrial pathway
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Pieniazek, Anna, Bernasinska-Slomczewska, Joanna, and Hikisz, Pawel
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- 2023
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5. ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organization
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Wu, Ambroise, Mazurkiewicz, Ewa, Donizy, Piotr, Kotowski, Krzysztof, Pieniazek, Małgorzata, Mazur, Antonina J., Czogalla, Aleksander, and Trombik, Tomasz
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- 2023
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6. Texture study of gluten-free cookies added with fibre by instrumental, image and sensory analysis
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Gagneten, Maite, Pieniazek, Facundo, Archaina, Diego, Messina, Valeria, Salvatori, Daniela, and Schebor, Carolina
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- 2023
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7. Modulation of the Human Erythrocyte Antioxidant System by the 5- and 6-Membered Heterocycle-Based Nitroxides
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Krzysztof Gwozdzinski, Stella Bujak-Pietrek, Anna Pieniazek, and Lukasz Gwozdzinski
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nitroxide ,red blood cells ,oxidative stress ,lactate dehydrogenase ,superoxide dismutase ,catalase ,Organic chemistry ,QD241-441 - Abstract
Nitroxides are stable radicals consisting of a nitroxyl group, >N-O•, which carries an unpaired electron. This group is responsible for the paramagnetic and antioxidant properties of these compounds. A recent study evaluated the effects of pyrrolidine and pyrroline derivatives of nitroxides on the antioxidant system of human red blood cells (RBCs). It showed that nitroxides caused an increase in the activity of superoxide dismutase (SOD) and the level of methemoglobin (MetHb) in cells (in pyrroline derivatives) but had no effect on the activity of catalase and lactate dehydrogenase. Nitroxides also reduced the concentration of ascorbic acid (AA) in cells but did not cause any oxidation of proteins or lipids. Interestingly, nitroxides initiated an increase in thiols in the plasma membranes and hemolysate. However, the study also revealed that nitroxides may have pro-oxidant properties. The drop in the AA concentration and the increase in the MetHb level and in SOD activity may indicate the pro-oxidant properties of nitroxides in red blood cells.
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- 2024
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8. Indoxyl sulfate induces apoptosis in mononuclear blood cells via mitochondrial pathway
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Anna Pieniazek, Joanna Bernasinska-Slomczewska, and Pawel Hikisz
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Medicine ,Science - Abstract
Abstract The consequence of chronic kidney disease is the accumulation of metabolic products called uremic toxins in the body. Indoxyl sulfate (IS) is a toxin with a high affinity for proteins. This study focuses on the deleterious effect of IS, especially apoptosis induction, in mononuclear blood cells (MNCs). Thus, in MNCs treated with IS at three different concentrations for 24 h, the survival, mitochondrial potential, caspases activity and expression, Bcl-2 and Bax protein expression, DNA damage, and PARP degradation were estimated. The study showed a decrease in survival and mitochondrial potential of MNCs treated with IS compared to the control. IS increased the activity of caspase 2-, 3-, 9-, and the expression of caspase 3-, and 9- in MNCs but does not affect the activity of caspase 6- and 8. The treatment of MNCs with IS also increased DNA damage and degradation of PARP. Indoxyl sulfate significantly influences the expression of Bcl-2 and Bax proteins. Indoxyl sulfate induces the programmed death of MNCs through the intrinsic mitochondrial apoptotic pathway. The observed cellular changes are mostly dose-dependent.
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- 2023
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9. ABCA1 transporter promotes the motility of human melanoma cells by modulating their plasma membrane organization
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Ambroise Wu, Ewa Mazurkiewicz, Piotr Donizy, Krzysztof Kotowski, Małgorzata Pieniazek, Antonina J. Mazur, Aleksander Czogalla, and Tomasz Trombik
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ABCA1 ,Cell motility ,Cholesterol ,Human melanoma ,Plasma membrane ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background Melanoma is one of the most aggressive and deadliest skin tumor. Cholesterol content in melanoma cells is elevated, and a portion of it accumulates into lipid rafts. Therefore, the plasma membrane cholesterol and its lateral organization might be directly linked with tumor development. ATP Binding Cassette A1 (ABCA1) transporter modulates physico-chemical properties of the plasma membrane by modifying cholesterol distribution. Several studies linked the activity of the transporter with a different outcome of tumor progression depending on which type. However, no direct link between human melanoma progression and ABCA1 activity has been reported yet. Methods An immunohistochemical study on the ABCA1 level in 110 patients-derived melanoma tumors was performed to investigate the potential association of the transporter with melanoma stage of progression and prognosis. Furthermore, proliferation, migration and invasion assays, extracellular-matrix degradation assay, immunochemistry on proteins involved in migration processes and a combination of biophysical microscopy analysis of the plasma membrane organization of Hs294T human melanoma wild type, control (scrambled), ABCA1 Knockout (ABCA1 KO) and ABCA1 chemically inactivated cells were used to study the impact of ABCA1 activity on human melanoma metastasis processes. Results The immunohistochemical analysis of clinical samples showed that high level of ABCA1 transporter in human melanoma is associated with a poor prognosis. Depletion or inhibition of ABCA1 impacts invasion capacities of aggressive melanoma cells. Loss of ABCA1 activity partially prevented cellular motility by affecting active focal adhesions formation via blocking clustering of phosphorylated focal adhesion kinases and active integrin β3. Moreover, ABCA1 activity regulated the lateral organization of the plasma membrane in melanoma cells. Disrupting this organization, by increasing the content of cholesterol, also blocked active focal adhesion formation. Conclusion Human melanoma cells reorganize their plasma membrane cholesterol content and organization via ABCA1 activity to promote motility processes and aggressiveness potential. Therefore, ABCA1 may contribute to tumor progression and poor prognosis, suggesting ABCA1 to be a potential metastatic marker in melanoma.
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- 2023
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10. Classification, quality characteristics, sensory perception and texture prediction of wooden breast myopathy in broilers from Argentina
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López, Tamara Anahí, García, Patricio Antonio, Tica, Lautaro, Pieniazek, Facundo, Schebor, Carolina, and Sosa, Natalia
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- 2023
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11. Baicalin and Baicalein Enhance Cytotoxicity, Proapoptotic Activity, and Genotoxicity of Doxorubicin and Docetaxel in MCF-7 Breast Cancer Cells
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Joanna Bernasinska-Slomczewska, Pawel Hikisz, Anna Pieniazek, and Aneta Koceva-Chyla
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baicalin ,baicalein ,doxorubicin ,docetaxel ,cytotoxicity ,apoptosis ,Organic chemistry ,QD241-441 - Abstract
Breast cancer is a major health concern and the leading cause of death among women worldwide. Standard treatment often involves surgery, radiotherapy, and chemotherapy, but these come with side effects and limitations. Researchers are exploring natural compounds like baicalin and baicalein, derived from the Scutellaria baicalensis plant, as potential complementary therapies. This study investigated the effects of baicalin and baicalein on the cytotoxic, proapoptotic, and genotoxic activity of doxorubicin and docetaxel, commonly used chemotherapeutic drugs for breast cancer. The analysis included breast cancer cells (MCF-7) and human endothelial cells (HUVEC-ST), to assess potential effects on healthy tissues. We have found that baicalin and baicalein demonstrated cytotoxicity towards both cell lines, with more potent effects observed in baicalein. Both flavonoids, baicalin (167 µmol/L) and baicalein (95 µmol/L), synergistically enhanced the cytotoxic, proapoptotic, and genotoxic activity of doxorubicin and docetaxel in breast cancer cells. In comparison, their effects on endothelial cells were mixed and depended on concentration and time. The results suggest that baicalin and baicalein might be promising complementary agents to improve the efficacy of doxorubicin and docetaxel anticancer activity. However, further research is needed to validate their safety and efficacy in clinical trials.
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- 2024
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12. Acrolein Induces Changes in Cell Membrane and Cytosol Proteins of Erythrocytes
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Michal Kopera, Krzysztof Gwozdzinski, and Anna Pieniazek
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acrolein ,erythrocytes ,lipid membrane fluidity ,thiol group ,amino group ,Organic chemistry ,QD241-441 - Abstract
High concentrations of acrolein (2-propenal) are found in polluted air and cigarette smoke, and may also be generated endogenously. Acrolein is also associated with the induction and progression of many diseases. The high reactivity of acrolein towards the thiol and amino groups of amino acids may cause damage to cell proteins. Acrolein may be responsible for the induction of oxidative stress in cells. We hypothesized that acrolein may contribute to the protein damage in erythrocytes, leading to the disruption of the structure of cell membranes. The lipid membrane fluidity, membrane cytoskeleton, and osmotic fragility were measured for erythrocytes incubated with acrolein for 24 h. The levels of thiol, amino, and carbonyl groups were determined in cell membrane and cytosol proteins. The level of non-enzymatic antioxidant potential (NEAC) and TBARS was also measured. The obtained research results showed that the exposure of erythrocytes to acrolein causes changes in the cell membrane and cytosol proteins. Acrolein stiffens the cell membrane of erythrocytes and increases their osmotic sensitivity. Moreover, it has been shown that erythrocytes treated with acrolein significantly reduce the non-enzymatic antioxidant potential of the cytosol compared to the control.
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- 2024
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13. Misclassification of carotid stenosis severity with area stenosis-based evaluation by computed tomography angiography: impact on erroneous indication to revascularization or patient (lesion) migration to a higher guideline recommendation class as per ESC/ESVS/ESO/SVS and CMS-FDA thresholds
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Lukasz Tekieli, Adam Mazurek, Karolina Dzierwa, Justyna Stefaniak, Anna Kablak-Ziembicka, Magdalena Knapik, Zbigniew Moczulski, R. Pawel Banys, Malgorzata Urbanczyk-Zawadzka, Wladyslaw Dabrowski, Maciej Krupinski, Piotr Paluszek, Ewa Weglarz, Łukasz Wiewiórka, Mariusz Trystula, Tadeusz Przewlocki, Piotr Pieniazek, and Piotr Musialek
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carotid stenosis severity ,percentage stenosis ,computed tomography angiography ,catheter angiography ,area stenosis ,diameter stenosis ,Medicine - Published
- 2023
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14. Factors Influencing Venous Remodeling in the Development of Varicose Veins of the Lower Limbs
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Lukasz Gwozdzinski, Anna Pieniazek, and Krzysztof Gwozdzinski
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chronic venous insufficiency ,varicose vein ,pathophysiology ,inflammation ,endothelial dysfunction ,oxidative damage ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
One of the early symptoms of chronic venous disease (CVD) is varicose veins (VV) of the lower limbs. There are many etiological environmental factors influencing the development of chronic venous insufficiency (CVI), although genetic factors and family history of the disease play a key role. All these factors induce changes in the hemodynamic in the venous system of the lower limbs leading to blood stasis, hypoxia, inflammation, oxidative stress, proteolytic activity of matrix metalloproteinases (MMPs), changes in microcirculation and, consequently, the remodeling of the venous wall. The aim of this review is to present current knowledge on CVD, including the pathophysiology and mechanisms related to vein wall remodeling. Particular emphasis has been placed on describing the role of inflammation and oxidative stress and the involvement of extracellular hemoglobin as pathogenetic factors of VV. Additionally, active substances used in the treatment of VV were discussed.
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- 2024
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15. The Effect of Piperidine Nitroxides on the Properties of Metalloproteins in Human Red Blood Cells
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Stella Bujak-Pietrek, Anna Pieniazek, Krzysztof Gwozdzinski, and Lukasz Gwozdzinski
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nitroxide ,red blood cells ,oxidative stress ,lactate dehydrogenase ,methemoglobin ,Organic chemistry ,QD241-441 - Abstract
Nitroxides are stable, low molecular-weight radicals containing a nitroxide group that has an unpaired electron. The presence of a nitroxide group determines their redox properties. The effect of the piperidine nitroxides, Tempo, Tempol, and Tempamine, on metalloproteins (hemoglobin, superoxide dismutase, catalase) and lactate dehydrogenase in red blood cells was investigated in this research. In addition, the level of lipid peroxidation and the level of protein carbonyl groups were examined as indicators of the effect of oxidative stress. Nitroxides increased superoxide dismutase activity and oxidized hemoglobin to methemoglobin, and also slightly decreased the catalase activity of red blood cells treated with nitroxides. Tempol significantly decreased lactate dehydrogenase activity. All three nitroxides had no effect on membrane lipid peroxidation and protein oxidation. Our results confirm that nitroxides have both antioxidant and prooxidative effects in human red blood cells. The piperidine nitroxides do not initiate the oxidation of proteins and lipids in the membranes of human red blood cells.
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- 2023
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16. The Effect of Diosmin, Escin, and Bromelain on Human Endothelial Cells Derived from the Umbilical Vein and the Varicose Vein—A Preliminary Study
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Lukasz Gwozdzinski, Joanna Bernasinska-Slomczewska, Pawel Hikisz, Anna Wiktorowska-Owczarek, Edward Kowalczyk, and Anna Pieniazek
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varicose vein ,human endothelial cells ,diosmin ,escin ,bromelain ,Biology (General) ,QH301-705.5 - Abstract
In this study, we investigated the properties of human varicose vein (VV) endothelial cells (HVVEC) in comparison to the human umbilical vein endothelial cells (HUVEC). The cells were treated with three bioactive compounds with proven beneficial effects in the therapy of patients with VV, diosmin, escin, and bromelain. Two concentrations of tested drugs were used (1, 10 mg/mL), which did not affect the viability of either cell type. Escin led to a slight generation of reactive oxygen species in HUVEC cells. We observed a slight release of superoxide in HVVEC cells upon treatment with diosmin and escin. Diosmin and bromelain showed a tendency to release nitric oxide in HUVEC. Using membrane fluorescent probes, we demonstrated a reduced fluidity of HVVEC, which may lead to their increased adhesion, and, consequently, a much more frequent occurrence of venous thrombosis. For the first time, we show the mechanism of action of drugs used in VV therapy on endothelial cells derived from a VV. Studies with HVVEC have shown that tested drugs may lead to a reduction in the adhesive properties of these cells, and thus to a lower risk of thrombosis.
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- 2023
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17. Magnetic anisotropy induced by crystal distortion in Ge1-xMn xTe/PbTe//KCl (001) ferromagnetic semiconductor layers
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Knoff, W., Łusakowski, A., Domagała, J. Z., Minikayev, R., Taliashvili, B., Łusakowska, E., Pieniążek, A., Szczerbakow, A., and Story, T.
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Condensed Matter - Materials Science - Abstract
Ferromagnetic resonance (FMR) study of magnetic anisotropy is presented for thin layers of IV-VI diluted magnetic semiconductor Ge1-xMn xTe with x=0.14 grown by molecular beam epitaxy (MBE) on KCl (001) substrate with a thin PbTe buffer. Analysis of the angular dependence of the FMR resonant field reveals that an easy magnetization axis is located near to the normal to the layer plane and is controlled by two crystal distortions present in these rhombohedral Ge1-xMnxTe layers: the ferroelectric distortion with the relative shift of cation and anion sub-lattices along the [111] crystal direction and the biaxial in-plane, compressive strain due to thermal mismatch.
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- 2015
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18. Diosmin and Bromelain Stimulate Glutathione and Total Thiols Production in Red Blood Cells
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Lukasz Gwozdzinski, Joanna Bernasinska-Slomczewska, Anna Wiktorowska-Owczarek, Edward Kowalczyk, and Anna Pieniazek
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diosmin ,bromelain ,red blood cells ,oxidative stress ,Organic chemistry ,QD241-441 - Abstract
Diosmin and bromelain are bioactive compounds of plant origin with proven beneficial effects on the human cardiovascular system. We found that diosmin and bromelain slightly reduced total carbonyls levels and had no effect on TBARS levels, as well as slightly increased the total non-enzymatic antioxidant capacity in the RBCs at concentrations of 30 and 60 µg/mL. Diosmin and bromelain induced a significant increase in total thiols and glutathione in the RBCs. Examining the rheological properties of RBCs, we found that both compounds slightly reduce the internal viscosity of the RBCs. Using the MSL (maleimide spin label), we revealed that higher concentrations of bromelain led to a significant decrease in the mobility of this spin label attached to cytosolic thiols in the RBCs, as well as attached to hemoglobin at a higher concentration of diosmin, and for both concentrations of bromelain. Both compounds tended to decrease the cell membrane fluidity in the subsurface area, but not in the deeper regions. An increase in the glutathione concentration and the total level of thiol compounds promotes the protection of the RBCs against oxidative stress, suggesting that both compounds have a stabilizing effect on the cell membrane and improve the rheological properties of the RBCs.
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- 2023
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19. Outcome of the First wwPDB/CCDC/D3R Ligand Validation Workshop
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Adams, Paul D, Aertgeerts, Kathleen, Bauer, Cary, Bell, Jeffrey A, Berman, Helen M, Bhat, Talapady N, Blaney, Jeff M, Bolton, Evan, Bricogne, Gerard, Brown, David, Burley, Stephen K, Case, David A, Clark, Kirk L, Darden, Tom, Emsley, Paul, Feher, Victoria A, Feng, Zukang, Groom, Colin R, Harris, Seth F, Hendle, Jorg, Holder, Thomas, Joachimiak, Andrzej, Kleywegt, Gerard J, Krojer, Tobias, Marcotrigiano, Joseph, Mark, Alan E, Markley, John L, Miller, Matthew, Minor, Wladek, Montelione, Gaetano T, Murshudov, Garib, Nakagawa, Atsushi, Nakamura, Haruki, Nicholls, Anthony, Nicklaus, Marc, Nolte, Robert T, Padyana, Anil K, Peishoff, Catherine E, Pieniazek, Susan, Read, Randy J, Shao, Chenghua, Sheriff, Steven, Smart, Oliver, Soisson, Stephen, Spurlino, John, Stouch, Terry, Svobodova, Radka, Tempel, Wolfram, Terwilliger, Thomas C, Tronrud, Dale, Velankar, Sameer, Ward, Suzanna C, Warren, Gregory L, Westbrook, John D, Williams, Pamela, Yang, Huanwang, and Young, Jasmine
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Generic health relevance ,Crystallography ,X-Ray ,Data Curation ,Databases ,Protein ,Guidelines as Topic ,Ligands ,Models ,Molecular ,Protein Conformation ,Proteins ,Biophysics - Abstract
Crystallographic studies of ligands bound to biological macromolecules (proteins and nucleic acids) represent an important source of information concerning drug-target interactions, providing atomic level insights into the physical chemistry of complex formation between macromolecules and ligands. Of the more than 115,000 entries extant in the Protein Data Bank (PDB) archive, ∼75% include at least one non-polymeric ligand. Ligand geometrical and stereochemical quality, the suitability of ligand models for in silico drug discovery and design, and the goodness-of-fit of ligand models to electron-density maps vary widely across the archive. We describe the proceedings and conclusions from the first Worldwide PDB/Cambridge Crystallographic Data Center/Drug Design Data Resource (wwPDB/CCDC/D3R) Ligand Validation Workshop held at the Research Collaboratory for Structural Bioinformatics at Rutgers University on July 30-31, 2015. Experts in protein crystallography from academe and industry came together with non-profit and for-profit software providers for crystallography and with experts in computational chemistry and data archiving to discuss and make recommendations on best practices, as framed by a series of questions central to structural studies of macromolecule-ligand complexes. What data concerning bound ligands should be archived in the PDB? How should the ligands be best represented? How should structural models of macromolecule-ligand complexes be validated? What supplementary information should accompany publications of structural studies of biological macromolecules? Consensus recommendations on best practices developed in response to each of these questions are provided, together with some details regarding implementation. Important issues addressed but not resolved at the workshop are also enumerated.
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- 2016
20. A meta-analysis of the effect of stent design on clinical and radiologic outcomes of carotid artery stenting
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Bijuklic, K., Schofer, J., Bonati, L., Bosiers, M., Wauters, J., de Donato, G., Chisci, E., Setacci, C., Doig, D., Featherstone, R.L., Dobson, J., Brown, M.M., Eskandari, M.K., Giri, J., Grunwald, I.Q., Kühn, A.L., Han, D.K., Faries, P.L., Hernandez-Fernandez, F., Parrilla, G., Hornung, M., Sievert, H., Kono, K., Latacz, P., Ledwoch, J., Mudra, H., Maleux, G., Nolz, R., Ohki, T., Piazza, M., Pieniazek, P., Tekieli, L., Radak, D., Tanaskovic, S., Rasiova, M., Simonte, G., Fiorucci, B., Tietke, M.W.K., Ventoruzzo, G., de Vries, Evelien E., Meershoek, Armelle J.A., Vonken, Evert J., den Ruijter, Hester M., van den Berg, Jos C., and de Borst, Gert J.
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- 2019
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21. Simultaneous single-stage urgent cardiac surgery and endovascular carotid revascularization under open-chest cardiopulmonary bypass in extremely high-risk, unstable patients
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Dzierwa, K, primary, Kedziora, A, additional, Mazurek, A, additional, Tekieli, L, additional, Musial, R, additional, Dobrowolska, E, additional, Pieniazek, P, additional, Sobczynski, R, additional, Kapelak, B, additional, Kwiatkowski, T, additional, Trystula, M, additional, Piatek, J, additional, and Musialek, P, additional
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- 2023
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22. Comparison of drug-eluting and bare metal stents for extracranial vertebral artery stenting
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Damian Maciejewski, Piotr Pieniazek, Lukasz Tekieli, Piotr Paluszek, Tadeusz Przewlocki, Tomasz Tomaszewski, Roman Machnik, Mariusz Trystula, Jacek Legutko, and Anna Kablak-Ziembicka
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drug-eluting stent ,in-stent restenosis ,bare metal stent ,vertebral artery stenting ,Medicine - Published
- 2019
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23. The role of serial carotid intima-media thickness assessment as surrogate marker of atherosclerosis control in patients with recent myocardial infarction
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Jacek Gacoń, Tadeusz Przewłocki, Jakub Podolec, Rafal Badacz, Piotr Pieniazek, Wojciech Ryniewicz, Krzysztof Żmudka, and Anna Kabłak-Ziembicka
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carotid intima-media thickness ,coronary artery diseases ,symptom recurrence ,myocardial infarction ,atherosclerosis progression ,major adverse cerebral and coronary events ,Medicine - Published
- 2019
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24. Indoxyl Sulfate Induces Oxidative Changes in Plasma and Hemolysate
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Anna Pieniazek, Michal Kopera, Lukasz Gwozdzinski, and Krzysztof Gwozdzinski
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indoxyl sulfate ,hemolysate ,plasma ,oxidative stress ,Organic chemistry ,QD241-441 - Abstract
The deteriorating function of the kidneys in chronic kidney disease (CKD) is associated, among other things, with the retention of many unnecessary metabolic products in the body. Indoxyl sulfate (IS) belongs to the group of uremic toxins with a high protein binding affinity. Moreover, this compound can generate oxidative stress. We hypothesized that a high concentration of IS might induce oxidative changes in erythrocytes and plasma components, and could therefore contribute to CKD progression. In this study, we evaluated the influence of IS on the oxidative stress parameters in plasma and hemolysate. Moreover, as a result of the action of IS, we observed a decrease in the total antioxidant capacity and a change in the activity of catalase and superoxide dismutase in hemolysate and plasma. The obtained results indicate that IS induces oxidative damage to hemolysate and plasma components. Greater changes in the parameters of oxidative stress were observed in hemolysate than in plasma treated with indoxyl sulfate. The obtained results suggest that the increased concentration of IS in patients with chronic kidney disease may lead to a decrease in the lifespan of erythrocytes in their bloodstream.
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- 2022
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25. Alterations in the Plasma and Red Blood Cell Properties in Patients with Varicose Vein: A Pilot Study
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Lukasz Gwozdzinski, Anna Pieniazek, Joanna Bernasinska-Slomczewska, Pawel Hikisz, and Krzysztof Gwozdzinski
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
The varicose vein results from the inefficient functioning of the valves in the lower limb veins, making the blood flow slow down and leading to blood stasis and hypoxia. This type of vein dysfunction might be a result of the development of oxidative stress. We compared oxidative stress markers in the plasma and erythrocytes obtained from peripheral veins and varicose veins in the same patients (glutathione, nonenzymatic antioxidant capacity (NEAC), catalase (CAT) and acetylcholinesterase (AChE) activity, thiols, thiobarbituric acid-reactive substance (TBARS), and protein carbonyls). We found a decrease in NEAC in the plasma obtained from the varicose veins compared to the peripheral veins. We detected a decrease in thiols in the plasma, hemolysate, and plasma membranes and increase in protein carbonyl compounds and TBARS levels in the varicose veins. These changes were accompanied by a decrease in CAT and AChE activity. For the first time, our results show changes in the plasma, erythrocyte membrane, and hemolysate protein properties in varicose vein blood in contrast to the plasma and erythrocytes in peripheral vein blood from the same patients. The increased oxidative stress accompanying varicose vein disease might result from the local inefficiency of the antioxidant defense system.
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- 2021
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26. Differential Occurrence of Cuticular Wax and Its Role in Leaf Physiological Mechanisms of Three Edible Aroids of Northeast India
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Facundo Pieniazek, Madhumita Dasgupta, Valeria Messina, Mayengbam Premi Devi, Yumnam Indrani Devi, Sansuta Mohanty, Satyapriya Singh, Bibhuti Bhusan Sahoo, Potshangbam Nongdam, Gobinda Chandra Acharya, and Manas Ranjan Sahoo
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aroid leaves ,cuticular wax ,chlorophyll stability ,hydrophobicity ,physiological mechanisms ,sun protection factor ,Agriculture (General) ,S1-972 - Abstract
The localization of cuticular wax (CW) on the leaf epidermis and its interaction with the physiological mechanisms of three edible aroids, Alocasia, Colocasia, and Xanthosoma, were assessed. CW in the leaf tissues was visualized using scanning electron microscopy, which was higher in Colocasia (10.61 mg·dm−2) and Xanthosoma (11.36 mg·dm−2) than in Alocasia (1.36 mg·dm−2). Colocasia CW exhibited superhydrophobic properties with a higher static contact angle (CA) (>150°) than Xanthosoma (99.0°) and Alocasia (128.7°). The higher CW in Colocasia and Xanthosoma resulted in better leaf chlorophyll stability, moisture retention ability, and cellular membrane integrity compared to Alocasia. CW acted as a protecting barrier against deleterious solar radiation in terms of sun protection factor (SPF). The glossy appearance of wax crystals in the Alocasia leaf cuticles resulted in higher SPF. Overall, Colocasia CW highly influenced the qualitative and protective mechanisms of the leaf. Our study sheds light on the pivotal role of CW in the physiological properties of aroid leaves, which would be useful for the selection of wax-rich plants for augmenting future breeding strategies. The information would also be useful for further exploration of the industrial potential of superhydrophobic wax crystals obtained from edible aroids.
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- 2022
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27. Texture of Bananas Submitted to Different Freeze Drying Cycle Applying Scanning Electron Microsocopy with Image Analysis Techniques
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Andino, Agustina Roa, Pieniazek, Facundo, and Messina, Valeria
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- 2019
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28. Alterations in the Properties of Red Blood Cells in Men with Coronary Artery Diseases after Comprehensive Cardiac Rehabilitation
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Krzysztof Gwozdzinski, Anna Pieniazek, Joanna Bernasinska-Slomczewska, Joanna Brzeszczynska, Robert Irzmanski, and Anna Jegier
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Purpose. Comprehensive cardiac rehabilitation (CCR) is a complex program aimed at improving the health status of patients with coronary artery disease (CAD), especially those who have been subjected to cardiac interventions (PCI and CABG).The aim of this study was to measure the changes in the properties of red blood cells (RBCs) in men with CAD after cardiac intervention and after participation in CCR program. Methods. In this study, we have investigated the influence of the physical training-based CCR program in 12 men with CAD, after PCI or CABG. The characteristics of RBCs including the basic morphology of RBCs, the conformational state of RBC membrane protein and hemoglobin, acetylcholinesterase activity, membrane fluidity, the osmotic fragility, and thiol concentration in membrane and in hemolysate were measured. Ascorbate concentration and reduced glutathione were also determined. The analysis was performed in men, before and after participation in CCR. The properties of RBCs were observed in connection with the exercise test, and parameters were evaluated before, immediately after, and 1 hour after the exercise test. Results. After CCR, a decrease in the mobility of erythrocyte membrane proteins was observed, which was accompanied by a decrease in lipid fluidity. In addition, immediately after the exercise test and 1 hour later, we measured a decrease in thiol level in hemolysate, but not in the plasma membrane. Unexpectedly, an increase in reduced glutathione concentration one hour after the exercise test after completing comprehensive cardiac rehabilitation was observed. Conclusion. CCR in men with CAD after cardiac intervention is connected with decreased membrane fluidity and decreased membrane protein mobility, which indicates that reduction of oxidative changes in these components occurs.
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- 2020
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29. Field assessment of behavioural responses of southern stingrays (Hypanus americanus) to acoustic stimuli
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Megan F. Mickle, Rachel H. Pieniazek, and Dennis M. Higgs
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elasmobranchs ,bioacoustics ,behaviour ,southern stingray ,threshold ,frequency ,Science - Abstract
The ability of elasmobranchs to detect and use sound cues has been heavily debated in previous research and has only recently received revived attention. To properly understand the importance of sound to elasmobranchs, assessing their responses to acoustic stimuli in a field setting is vital. Here, we establish a behavioural audiogram of free-swimming male and female southern stingrays (Hypanus americanus) exposed to low-frequency tones. We demonstrate that female stingrays exposed to tones (50–500 Hz) exhibit significant changes in swimming behaviours (increased time spent swimming, decreased rest time, increased surface breaches and increased side swimming with pectoral flapping) at 140 dB re 1 µPa (−2.08 to −2.40 dB re 1 m s−2) while males exposed to the same tones did not exhibit a change in these behaviours until 160 dB re 1 µPa (−1.13 to −1.21 dB re 1 m s−2). Our results are the first demonstration of field responses to sound in the Batoidea and show a distinct sensitivity to low-frequency acoustic inputs.
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- 2020
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30. Alpha-Enolase (ENO1) Correlates with Invasiveness of Cutaneous Melanoma—An In Vitro and a Clinical Study
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Miriam Hippner, Michal Majkowski, Przemyslaw Biecek, Teresa Szkudlarek, Aleksandra Simiczyjew, Malgorzata Pieniazek, Dorota Nowak, Arkadiusz Miazek, and Piotr Donizy
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ENO1 ,cutaneous melanoma ,melanoma cell lines ,immunohistochemistry ,Medicine (General) ,R5-920 - Abstract
Alpha-enolase (ENO1) is a glycolytic metalloenzyme, and its overexpression occurs in numerous cancers, contributing to cancer cell survival, proliferation, and maintenance of the Warburg effect. Patients with an overexpression of ENO1 have a poor prognosis. The aim of the present study was to investigate the prognostic significance of ENO1 in surgical resections from 112 melanoma patients and to assess its expression and enzymatic activity in normoxia and hypoxia in several melanoma cell lines. Overexpression of ENO1 in tumor cells from patients was correlated with unfavorable prognosticators such as Breslow thickness, Clark level, mitotic activity, and the presence of ulceration. The expression of ENO1 also positively correlated with a greater thickness of the neoplastic infiltrate and a worse long-term prognosis for patients with cutaneous melanoma. We report significantly higher expression of ENO1 in melanoma cell lines in comparison to normal melanocytes. To conclude, our in vitro and clinical models showed that overexpression of ENO1 promotes invasiveness of melanoma cells and correlates with aggressive clinical behavior. These observations open the way to further search of a potential prognostic and therapeutic target in cutaneous melanoma.
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- 2022
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31. Immunomodulatory therapy in children with paediatric inflammatory multisystem syndrome temporally associated with SARS-CoV-2 (PIMS-TS, MIS-C; RECOVERY): a randomised, controlled, open-label, platform trial
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Faust, Saul N, Haynes, Richard, Jones, Christine E, Staplin, Natalie, Whittaker, Elizabeth, Jaki, Thomas, Juszczak, Ed, Spata, Enti, Wan, Mandy, Bamford, Alasdair, Dimitri, Paul, Finn, Adam, Furness, John, Ramanan, Athimalaipet V, Gale, Christopher, Cathie, Katrina, Drysdale, Simon, Bernatoniene, Jolanta, Murray, Clare, Roehr, Charles C, Fleming, Paul, Riordan, Andrew, Bandi, Srini, Jyothish, Deepthi, Evans, Jennifer, Emonts, Marieke, Kelly, Dominic, Pathan, Nazima, Davies, Patrick, Hague, Rosie, Pollock, Louisa, Semple, Malcolm G, Peto, Leon, Baillie, J Kenneth, Buch, Maya, Jeffery, Katie, Knight, Marian, Lim, Wei Shen, Montgomery, Alan, Mukherjee, Aparna, Mumford, Andrew, Rowan, Kathryn, Thwaites, Guy, Mafham, Marion, Emberson, Jonathan, Landray, Martin J, Horby, Peter W, Chappell, Lucy, Day, Jeremy, Dung, DV, Day, Jeremy, Quang, NN, Binh, Prof, Burhan, E, Alisjahbana, B, Koirala, J, Basnet, S, Kestelyn, E, Basnyat, B, Gyanwali, P, Hamers, RL, Sandercock, P, Darbyshire, J, DeMets, D, Fowler, R, Lalloo, D, Munavvar, M, Roberts, I, Warris, A, Wittes, J, Craddock-Bamford, A, Barton, J, Basoglu, A, Brown, R, Brudlo, W, Denis, E, Fletcher, L, Howard, S, Musini, S, Taylor, K, Cui, G, Goodenough, B, King, A, Lay, M, Murray, D, Stevens, W, Wallendszus, K, Welsh, R, Crichton, C, Davies, J, Goldacre, R, Harper, C, Knight, F, Nunn, M, Salih, H, Welch, J, Campbell, M, Pessoa-Amorim, G, Zayed, M, Wiles, J, Bagley, G, Cameron, S, Chamberlain, S, Farrell, B, Freeman, H, Kennedy, A, Whitehouse, A, Wilkinson, S, Wood, C, Reith, C, Davies, K, Halls, H, Holland, L, Truell, R, Wilson, K, Lingwood, Emma, Howie, L, Lunn, M, Rodgers, Penelope, Amuasi, J, Baird, K, Bao, T, Basnyat, B, Bittaye, M, Bonney, J, D'Alessandro, U, Dhimal, M, Gyanwali, P, Hamers, RL, Huyen, T, Jagne, A, Karkey, A, Kestelyn, E, Maiga, O, Matey, E, Nadjm, B, Nel, J, Pant, S, Puspatriani, K, Rahardjani, M, Raijal, S, Rees, H, Rimainar, A, Roberts, C, Rocca, A, Shrestha, S, Usuf, E, Vidaillic, C, Wulandari, F, Alexander, A, Amezaga, M, Armah, C, Asghar, A, Aubrey, P, Barker-Williams, K, Barnard, A, Bartholomew, J, Beaumont-Jewell, D, Beddows, S, Beety, J, Birch, C, Boyle, P, Brend, M, Brookes, M, Callens, C, Campbell, D, Campbell, H, Cate, H, Chambers, E, Chapman, K, Coe, A, Collins, J, Crawshaw, S, Das, S, De Soyza, A, Denton, E, Dicks, P, Dolman, M, Donaldson, K, Dowden, L, Dowling, S, Drake, C, Duckles-Leech, H, Duffy, H, Gerdes, L, Gibbons, K, Gill, L, Gilmour, K, Goodwin, J, Graham, J, Green, C, Hall, C, Hall, A, Hampshire, K, Hanson, J, Harman, T, Harper, H, Harrison, S, Hicks, J, Hodgson, H, Hough, M, Jackson, S, Johnson, G, Kirk, J, Lemoine, N, Levett, C, Lock, S, Lockett, T, Logan, M, Lomme, K, Luo, J, McMillan, N, Membrey, H, Mguni, N, Minton, J, Monaghan, H, Murphy, S, Muzengi, N, Naz, M, Nickson, I, Odam, M, Ohia, U, Okell, E, Oliver, A, Pearson, K, Pine, K, Pleass, R, Porter, D, Porter, L, Potter, A, Poustie, V, Pratt, S, Price, A, Priest, A, Rodgers, A, Rook, C, Ryan, P, Sam, S, Sharratt, P, Sheffield, J, Shenton, J, Shirley, K, Skinner, T, Slade, H, Sutu, M, Taylor, J, Thomas, E, Tinkler, H, Unsworth, A, Usher, R, van't Hoff, W, Walker, S, Walters, J, Walton, E, Warmington, J, Wayman, E, Williams, P, Williamson, J, Williamson, D, Yelnoorkar, F, Knight, M, Hodson, K, Pavord, S, Williamson, C, Bowman, L, Chen, F, Clarke, R, Goonasekara, M, Herrington, W, Judge, P, Ng, S, Preiss, D, Reith, C, Sammons, E, Zhu, D, Arkley, C, Bowker, P, Bruce, J, Byrne-Watts, V, Chapman, G, Coleman, G, Denwood, T, Gray, J, Harrison, D, Mather, N, Pinches, H, Rees, A, Rees, S, Turner, G, Abani, O, Abbas, A, Abbas, F, Abbas, J, Abbas, K, Abbas, M, Abbasi, S, Abbass, H, Abbott, A, Abdallah, N, Abdelaziz, A, Abdelfattah, M, Abdelqader, B, Abdul, A, Abdul, B, Abdul, S, Abdul Rasheed, A, Abdulakeem, A, Abdul-Kadir, R, Abdullah, A, Abdulmumeen, A, Abdul-Raheem, R, Abdulshukkoor, N, Abdusamad, K, Abed El Khaleq, Y, Abedalla, M, Abeer Ul Amna, A, Abel, L, Abernethy, K, Abeywickrema, M, Abhinaya, C, Aboaba, A, Aboagye-Odei, A, Aboelela, H, Abo-Leyah, H, Abouelela, K, Abou-Haggar, A, Abouibrahim, M, Abousamra, A, Abouzaid, M, Abraham, M, Abraham, T, Abraheem, A, Abrams, J, Abrams, R, Abu, H, Abu-Arafeh, A, Abubacker, S, Abung, A, Abusamra, Y, Aceampong, Y, Achara, A, Acharya, D, Acheampong, S, Acheampong, P, Acheson, J, Achieng, S, Acosta, A, Acquah, R, Acton, C, Adabie-Ankrah, J, Adair, P, Adam, F, Adam, M, Adamali, H, Adamczyk, M, Adams, C, Adams, D, Adams, K, Adams, L, Adams, N, Adams, R, Adams, T, Adamu-Ikeme, L, Adati, K, Adcock, K, Addo, A, Adeagbo, O, Adebiyi, A, Adedeji, O, Adegeye, Y, Adegoke, K, Adell, V, Adenwalla, S, Adeoye, F, Adesemoye, O, Adewunmi, E, Adeyanju, A, Adeyemi, J, Adeyemo, T, Adhikary, R, Aditya, A, Adkins, G, Adnan, A, Aeron-Thomas, J, Affleck, D, Afnan, C, Afridi, M, Aftab, Z, Agarwal, M, Agbeko, R, Agbo, C, Aggarwal, S, Aghababaie, A, Aguilar Jimenez, L, Ahamed Sadiq, S, Ahammed Nazeer, M, Ahmed, A, Ahmed, B, Ahmed, F, Ahmed, H, Ahmed, I, Ahmed, K, Ahmed, L, Ahmad, M, Ahmed, N, Ahmed, O, Ahmed, R, Ahmad, S, Ahmed, S H, Ahmed Ali, R, Ahmed Mohamud, B, Ahmer, S, Ahonia, A, Aiken, C, Ail, D, Ainsworth, M, Aissa, M, Aitken, L, Ajay, B, Ajibode, A, Ajmi, A, Akhtar, N, Akili, S, Akinbiyi, B, Akindolie, O, Akinfenwa, Y, Akinkugbe, O, Akinpelu, I, Akram, M, Aktinade, O, Akudi, U, Al Aaraj, A, Al Balushi, A, Al Dakhola, M, Al Swaifi, A, Al-Abadi, E, Alabi, A, Aladangady, N, Alafifi, M, Alam, A, Alam, S, Al-Asadi, A, Alatzoglou, K, Albert, P, Albon, L, Alcala, A, Alcorn, G, Alcorn, S, Aldana, A, Alderdice, D, Aldesouki, A, Aldouri, R, Aldridge, J, Aldridge, N, Alegria, A, Alexander, C, Alexander, J, Alexander, P, Al-Fori, J, Alghazawi, L, Alhabsha, O, Al-Hakim, B, Alhameed, R, Al-Hayali, M, Al-Hity, S, Ali, A, Ali, F, Ali, J, Ali, M, Ali, N, Ali, O, Ali, R, Ali, S, Aliberti, E, Alin, J, Alina, A, Alipustain, A, Aliyuda, F, Alizaedeh, K, Al-Jibury, M, Al-Juboori, S, Al-Khalil, M, Alkhudhayri, A, Alkhusheh, M, Allan, F, Allan, N, Allanson, A, Allcock, R, Allen, E, Allen, J, Allen, K, Allen, L, Allen, P, Allen, R, Allen, S, Allen, T, Alli, A, Allison, K, Allman, B, Allsup, D, Allsop, H, Allsop, L, Almahroos, A, Al-Moasseb, H, Al-Obaidi, M, Alomari, L, Al-Rabahi, A, Al-Ramadhani, B, Al-Saadi, Z, Al-Sammarraie, R, Alshaer, I, Al-Shahi Salman, R, Al-Shamkhani, W, Alsheikh, F, Al-Sheklly, B, Altaf, S, Alty, A, Alvarez, M, Alvarez Corral, M, Alveyn, E, Alzetani, M, Amamou, S, Amar, N, Ambalavanan, S, Ambrogetti, R, Ambrose, C, Ameen, A, Ames, K, Amin, A, Amin, K, Amin, S, Amin, T, Amit, B, Amjad, A, Amjad, N, Amosun, V, Amsal, M, Amsha, K, Amutio Martin, N, Amy, P, Anada, A, Anand, A, Anandappa, S, Anantapatnaikuni, S, Anderson, E, Anderson, J, Anderson, L, Anderson, M, Anderson, N, Anderson, R, Anderson, S, Anderson, W, Andreou, P, Andrews, A, Andrews, J, Andrews, J, Aneke, K, Ang, A, Ang, W, Angel, T, Angela, A, Angelini, P, Anguvaa, L, Anichtchik, O, Anim-Somuah, M, Aniruddhan, K, Annett, J, Anning, L, Anstey, P, Anstey, R, Anthony, A, Anthony-Pillai, A, Antill, P, Antonnia, Z, Anu, V, Anwar, M, Apetri, E, Apostolopoulos, A, Appleby, S, Appleyard, D, Aquino, M, Araba, B, Aransiola, S, Araujo, M, Archer, A, Archer, D, Archer, S, Arcoria, D, Ardley, C, Arias, A, Aribike, O, Arimoto, R, Armata, I, Armistead, J, Armitage, A, Armstrong, C, Armstrong, M, Armstrong, S, Armstrong, W, Armtrong, P, Arndt, H, Arnison-Newgass, C, Arnold, D, Arnold, R, Arnott, A, Arora, D, Arora, R, Arora, K, Arora, P, Arter, A, Arumaithurai, A, Arya, A, Arya, R, Asandei, D, Asghar, M, Ashab, M, Ashbrook-Raby, C, Ashby, H, Ashcroft, J, Ashcroft, S, Asher, G, Ashfak, Z, Ashfaq, A, Ashish, A, Ashley, D, Ashman-Flavell, S, Ashok, S, Ashour, A, Ashraf, S, Ashraf, M, Ashraq, M, Ashton, D, Ashton, S, Ashworth, A, Ashworth, F, Ashworth, R, Aslam, A, Aslam, I, Aslam, S, Aslett, L, Asogan, H, Asrar, A, Assaf, O, Astin-Chamberlain, R, Athorne, D, Atkins, B, Atkins, C, Atkins, S, Atkinson, J, Atkinson, V, Atomode, A, Atraskiewicz, B, Attia, A, Attubato, E, Attwood, M, Auer, Z, Aujayeb, A, Aung, A, Aung, H, Aung, K, Aung, N, Aung, Y, Aung, Z, Austin, E, Austin, K, Auwal, A, Avari, M, Avery, M, Aveyard, N, Avis, J, Aviss, G, Avram, C, Avram, P, Awadelkareem, A, Awadzi, G, Awaly, M, Awan, A, Awoseyila, A, Aya, A, Ayaz, E, Ayers, A, Azam, J, Azeem, A, Azharuddin, M, Aziz, A, Aziz, G, Aziz, I, Aziz, N, Azkoul, A, Azman Shah, A, Azzopardi, G, Azzoug, H, Babatunde, F, Babi, M, Babiker, B, Babington, G, Babirecki, M, Babores, M, Babs-Osibodu, A, Bacciarelli, S, Bachar, R, Bachour, M, Bacon, G, Bacon, J, Badal, B, Bader, M, Badhan, G, Badhrinarayanan, S, Bae, J, Baggaley, A, Baggott, A, Bagmane, D, Bagshaw, L, Bahadori, K, Bailey, A, Bailey, J, Bailey, K, Bailey, L, Bailey, M, Bailey, P, Bailey, S, Baillie, H, Baillie, J, Bain, J, Bains, V, Baird, D, Baird, E, Baird, S, Baird, T, Baird, T, Baird, Y, Bajandouh, A, Baker, D, Baker, E, Baker, J, Baker, J, Baker, K, Baker, M, Baker, R, Baker, T, Baker, V, Bakere, H, Bakerly, N, Baker-Moffatt, M, Bakhai, A, Bakhtiar, N, Bakoulas, P, Bakthavatsalam, D, Balachandran, N, Balan, A, Balasingam, P, Balaskas, T, Balasubramania, M, Balatoni, N, Balcombe, A, Baldwin, A, Baldwin, C, Baldwin, D, Baldwin, F, Baldwin, A, Baldwin-Jones, R, Bale, N, Balfour, J, Ball, R, Ball, M, Ballard, K, Balluz, I, Balmforth, C, Balogh, E, Baltmr, A, Baluwala, A, Bambridge, G, Bamford, P, Bamford, A, Bamgboye, A, Bancroft, H, Bancroft, E, Banda, J, Bandaru, K, Bandla, N, Bandyopadhyam, S, Banerjee, A, Banerjee, R, Bani-Saad, O, Banks, H, Banks, L, Banks, P, Bann, C, Bannister, H, Bannister, O, Banton, L, Baptist, M, Baqai, T, Baral, A, Baramova, D, Barbe, R, Barbon, E, Barbosa, M, Barbour, J, Barclay, A, Barclay, A, Barclay, C, Bardsley, G, Bareford, S, Bari, S, Barker, A, Barker, D, Barker, E, Barker, H, Barker, J, Barker, L, Barker, O, Barker-Williams, K, Barkha, S, Barla, J, Barlow, G, Barlow, R, Barlow, V, Barnacle, J, Barnes, D, Barnes, N, Barnes, R, Barnes, T, Barnetson, C, Barnett, A, Barnett-Vanes, A, Barnsley, W, Barr, A, Barr, C, Barr, D, Barr, J, Barratt, N, Barratt, S, Barrera, M, Barrett, A, Barrett, F, Barrett, J, Barrett, S, Barrow, E, Bartlett, C, Bartlett, G, Bartlett, J, Bartlett, L, Bartley, S, Bartolmeu-Pires, S, Barton, A, Barton, G, Barton, L, Barton, R, Baruah, R, Baryschpolec, S, Bashir, H, Bashyal, A, Basker, B, Basran, A, Bassett, G, Bassett, J, Bassford, C, Bassoy, B, Bastion, V, Basumatary, A, Basvi, P, Bataduwaarachchi, V, Bate, T, Bateman, H, Bateman, K, Bateman, V, Bates, E, Bates, H, Bates, M, Bates, S, Batham, S, Batista, A, Batla, A, Batra, D, Batty, A, Batty, H, Batty, T, Baum, M, Baumbe, R, Bautista, C, Bawa, T, Bawa, F, Bawani, F, Bax, S, Baxter, M, Baxter, N, Baxter, Z, Bayes, H, Bayo, L-A, Bazari, F, Bazaz, R, Bazli, A, Beacham, L, Beadles, W, Beadon, K, Beak, P, Beale, A, Beard, K, Beardsal, K, Bearpark, J, Beasley, A, Beattie, S, Beaumont, K, Beaver, T, Beavis, S, Beazley, C, Beck, S, Beckett, V, Beckitt, R, Beckley, S, Beddall, 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Thompson, L, Thompson, M, Thompson, O, Thompson, R, Thompson, Y, Thomson, N, Thorburn, P, Thorn, N, Thorne, C, Thorne, N, Thornton, A, Thornton, D, Thornton, J, Thornton, R, Thornton, S, Thornton, T, Thorpe, S, Thorpe, C, Thorpe, N, Thozthumparambil, P, Thrasyvoulou, L, Thraves, H, Thueux, G, Thu-Ta, P, Thwaiotes, V, Tiberi, S, Tieger, S, Tierney, C, Tighe, M, Tilbey, S, Till, C, Tiller, A, Tiller, H, Timerick, J, Timlick, E, Timmins, A, Timmis, A, Timms, H, Timoroksa, A, Tinashe, S, Tingley, S, Tinker, N, Tinkler, M, Tipper, J, Tirumalai Adisesh, A, Tivenan, H, Tluchowska, K, T-Michael, H, Todd, A, Todd, J, Todd, S, Toffoletti, O, Tohfa, M, Tohill, S, Tolson, M, Tomasova, N, Tomlin, S, Tomlins, S, Tomlinson, J, Tomlinson, K, Tonkin, J, Tonna, I, Toohey, C, Topham, K, Topping, M, Torokwa, A, Torrance, C, Touma, O, Tous Sampol, L, Tousis, R, Tout, M, Tovey, P, Towersey, G, Townley, J, Tozer, R, Tran, M, Tranter, H, Travers, J, Travill, C, Traynor, S, Trethowan, L, Treus Gude, E, 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Rasool, M, Uriel, A, Urruela, S, Uru, H, Usher, J, Usher, M, Usher-Rea, A, Ustianowski, A, Uzu, H, Vaccari, L, Vaghela, U, Vaidya, A, Vail, D, Valecka, B, Valentine, J, Valeria, B, Vallabhaneni, P, Vallotton, N, Vamplew, L, Vamvakiti, E, Vamvakopoulos, J, van Bruggen, L, van de Venne, M, van der Meer, A, van der Stelt, N, van Koutrik, L, Van Loggerenberg, A, Vance-Daniel, J, Vancheeswaran, R, Vandeyoon, S, Vankayalapati, P, Vanmali, P, Vansomeren, C, Vara, S, Vardy, S, Varghese, A, Varghese, M, Varney, W, Varnier, G, Varouxaki, A, Varquez, R, Vasadi, V, Vassell, K, Vassell, O, Vasu, V, Vasudevan, V, Vatish, M, Vaughan, S, Vayalaman, H, Vayapooree, D, Vaz, C, Veale, N, Veerasamy, S, Velankar, S, Velauthar, L, Veli, N, Vella, N, Velugupati, A, Velusamy, A, Venables, I, Venditti, M, Venkataramakrishnan, R, Venn, R, Ventilacion, L, Vere, J, Veres, M, Vergnano, S, Verling, W, Verma, A, Vernall, R, Vernon, B, Vertue, M, Verueco, L, Verula, J, Vethanayagam, N, Vettikumaran, S, Veys, L, Vickers, C, Victor, S, Victoria, S, Vidler, J, Vijayakumar, B, Vijayaraghavan Nalini, V, Vilcinskaite, B, Vileito, A, Vilimiene, N, Vinall, L, Vinay, S, Vinayakarao, L, Vincent, O, Vincent, R, Virdee, P, Virgilio, E, Virk, A, Visentin, E, Vitaglione, M, Vithian, K, Vittoria, S, Vivekananthan, S, Vlad, E, Vlies, B, von Oven, L, Vooght, C, Vutipongsatorn, K, Vuylsteke, A, Vyras, E, W Hay, A, Wach, R, Wadams, B, Wadd, S, Waddington, N, Wade, P, Wadsley, J, Wadsworth, K, Wafa, S, Wagstaff, D, Wagstaff, L, Wahab, D, Wahbi, Z, Waheed Adigun, A, Waidyanatha, S, Waite, A, Wake, R, Wakefield, A, Wakeford, W, Wakinshaw, F, Waldeck, E, Walden, A, Walding, L, Waldron, A, Waldron, J, Wales, E, Wali, B, Walker, D, Walker, G, Walker, H, Walker, I, Walker, K, Walker, L, Walker, O, Walker, R, Walker, S, Wallace, G, Wallbutton, R, Wallen, J, Waller, A, Waller, R, Wallis, G, Wallis, L, Wallis, M, Walmsley, E, Walsh, D, Walsh, E, Walsh, L, Walstow, D, Walter, D, Walters, A, Walters, H, Walters, J, Walton, E, Walton, L, Walton, M, Walton, O, Walton, S, Wands, M, Wane, R, Wang, F, Wang, N, Wang, R, Wang, S, Warbrick, D, Warburton, S, Ward, C, Ward, D, Ward, E, Ward, H, Ward, J, Ward, L, Ward, N, Ward, R, Ward, T, Warden, S, Wardere, G, Wardle, S, Wardy, H, Waring, G, Waring, S, Warner, B, Warner, C, Warnock, L, Warran, S, Warren, J, Warren, L, Warren, R, Warren, Y, Warrender, D, Warren-Miell, H, Warwick, G, Wassall, H, Wasson, E, Wastson, E, Watchorn, H, Waterfall, H, Waters, A, Waters, D, Waterstone, M, Watkin, A, Watkins, C, Watkins, E, Watkins, K, Watkins, L, Watson, A, Watson, E, Watson, F, Watson, J, Watson, K, Watson, L, Watson, P, Watson, R, Watters, M, Watterson, D, Watts, D, Watts, J, Watts, M, Waugh, V, Wayman, E, Wayman, M, Wazir, A, Weatherhead, M, Weatherly, N, Webb, C, Webb, H, Webb, K, Webb, S, Websdale, C, Webster, D, Webster, I, Webster, J, Webster, T, Wedlin, J, Wee, L, Weerakoon, R, Weerasinghe, T, Weeratunga, J, Weetman, M, Wei, S, Weichert, I, Welch, E, Welch, H, Welch, L, Welch, S, Welham, B, Weller, S, Wellings, L, Wells, B, Wellstead, S, Welsh, B, Welters, I, Welton, R, Wenn, V, Wentworth, L, Wesonga, J, Wesseldine, K, West, J, West, M, West, R, West, S, Western, L, Westhead, R, Weston, H, Westwood, A, Westwood, K, Westwood, S, Wetherill, B, Wheaver, S, Wheeler, H, Whelan, B, Whelband, M, Whileman, A, Whitcher, A, White, A, White, B, White, C, White, D, White, J, White, K, White, M, White, N, White, S, White, T, Whitehead, C, Whitehorn, K, Whitehouse, C, Whitehouse, T, Whiteley, J, Whiteley, L, Whiteley, S, Whitham, R, Whitlingum, G, Whitmore, D, Whittam, L, Whittington, A, Whittle, H, Whittle, R, Wiafe, E, Wiblin, L, Wickens, O, Widdrington, J, Wieboldt, J, Wieringa, H, Wiesender, C, Wiffen, L, Wight, A, Wignall, A, Wignall, C, Wilce, A, Wilcock, D, Wilcock, E, Wilcox, L, Wild, B, Wild, L, Wild, S, Wilde, M, Wilding, L, Wilding, P, Wildsmith, T, Wileman, J, Wiles, K, Wilhelmsen, E, Wiliams, T, Wilkie, J, Wilkin, D, Wilkins, H, Wilkins, J, Wilkins, S, Wilkinson, I, Wilkinson, L, Wilkinson, N, Wilkinson, S, Wilkinson, T, Willetts, S, Williams, A, Williams, C, Williams, D, Williams, E, Williams, G, Williams, H, Williams, J, Williams, K, Williams, M, Williams, P, Williams, R, Williams, S, Williams, T, Williamson, A, Williamson, C, Williamson, H, Williamson, J, Williamson, R, Willis, E, Willis, H, Willis, J, Wills, L, Willsher, L, Willshire, C, Wilson, A, Wilson, B, Wilson, D, Willson, F, Wilson, I, Willson, J, Wilson, K, Wilson, K-A, Wilson, L, Wilson, M, Wilson, T, Win, K, Win, M, Win, T, Win, W, Winckworth, L, Winder, L, Winder, P, Winearl, S, Winmill, H, Winn, S, Winpenny, C, Winslow, H, Winter, H, Winter, J, Winter-Goodwin, B, Winterton, J, Winwood, H, Wischhusen, J, Wisdom, S, Wise, M, Wiselka, M, Wiseman, R, Wiseman, S, Wishart, S, Wishlade, T, Witele, E, Withers, N, Wixted, D, Wodehouse, T, Wolf, W, Wolff, N, Wolffsohn, K, Wolf-Roberts, R, Wolodimeroff, E, Wolstencroft, A, Wong, A, Wong, C, Wong, C H, Wong, E, Wong, J, Wong, K, Wong, M, Wong, N, Wong, S, Wong, T, Wood, A, Wood, D, Wood, F, Wood, G, Wood, H, Wood, J, Wood, L, Wood, M, Wood, S, Wood, T, Woodall, K, Woodfield, R, Woodford, C, Woodford, E, Woodford, J, Woodhead, L, Woodhead, T, Woodland, P, Woodman, M, Woodmansey, S, Woods, C, Woods, J, Woods, K, Woods, S, Woodward, Z, Woolcock, M, Wooldridge, G, Woolf, R, Woollard, C, Woollen, L, Woolley, E, Woolley, J, Woosey, D, Wootton, D, Wootton, J, Worley, D, Worton, S, Wraight, J, Wray, M, Wren, K, Wren, L, Wrey Brown, C, Wright, C, Wright, D, Wright, F, Wright, H, Wright, I, Wright, L, Wright, R, Wright, S, Wright, T, Wroe, C, Wroe, H, Wu, H, Wu, P, Wubetu, J, Wulandari, R, Wurie, S, Wyatt, C, Wyn-Griffiths, F, Wynter, I, Xavier, B, Xhikola, A, Xia, B, Xia, Z, Y Ellis, T, Yacoba, E, Yakubi, M, Yan, M, Yanagisawa, Y, Yang, F, Yang, Y, Yanney, M, Yap, W, Yaqoob, N, Yasmin, S, Yates, B, Yates, D, Yates, E, Yates, H, Yates, M, Yates, T, Ye, J, Yearwood Martin, C, Yein, K, Yelnoorkar, F, Yeoh, A, Yeung, C, Yew, P, Yewatkar, D, Ylquimiche Melly, L, Ynter, I, Yong, H, Yorke, J, Youens, J, Younes Ibrahim, A, Young, E, Young, G, Young, L, Yousafzar, A, Youssouf, S, Yousuf, A, Yu, C, Yuan, J, Yung, B, Yusef, D, Yusef, S, Yusuf, I, Zafar, A-M, Zagalo, S, Zaher, S, Zahoor, A, Zainab, M, Zak, T, Zaki, K, Zakir, N, Zalewska, K, Zamalloa, A, Zaman, M, Zaman, S, Zamikula, J, Zammit, L, Zammit-Mangion, M, Zawadzka, M, Zebracki, E, Zehnder, D, Zeidan, L, Zeinali, D, Zhang, J, Zhao, X, Zheng, D, Zia, M, Zibdeh, O, Zill-E-Huma, R, Zin, E, Zincone, E, Zindoga, G, Zinkin, E, Zinyemba, V, Zipitis, C, Zitter, L, Zmierczak, A, Zubikarai, G, Zubir, A, Zuhra, N, Zulaikha, R, Zulfikar, S, Zullo, C, and Zuriaga-Alvaro, A
- Abstract
Paediatric multisystem inflammatory syndrome temporally associated with SARS-CoV-2 (PIMS-TS), also known as multisystem inflammatory syndrome in children (MIS-C) emerged in April, 2020. The paediatric comparisons within the RECOVERY trial aimed to assess the effect of intravenous immunoglobulin or corticosteroids compared with usual care on duration of hospital stay for children with PIMS-TS and to compare tocilizumab (anti-IL-6 receptor monoclonal antibody) or anakinra (anti-IL-1 receptor antagonist) with usual care for those with inflammation refractory to initial treatment.
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- 2024
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32. Uremic Toxins and Their Relation with Oxidative Stress Induced in Patients with CKD
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Anna Pieniazek, Joanna Bernasinska-Slomczewska, and Lukasz Gwozdzinski
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chronic kdney disease ,uremic toxins ,oxidative stress ,carbamylation ,cardiovascular disease ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The presence of toxins is believed to be a major factor in the development of uremia in patients with chronic kidney disease (CKD) and end-stage renal disease (ESRD). Uremic toxins have been divided into 3 groups: small substances dissolved in water, medium molecules: peptides and low molecular weight proteins, and protein-bound toxins. One of the earliest known toxins is urea, the concentration of which was considered negligible in CKD patients. However, subsequent studies have shown that it can lead to increased production of reactive oxygen species (ROS), and induce insulin resistance in vitro and in vivo, as well as cause carbamylation of proteins, peptides, and amino acids. Other uremic toxins and their participation in the damage caused by oxidative stress to biological material are also presented. Macromolecules and molecules modified as a result of carbamylation, oxidative stress, and their adducts with uremic toxins, may lead to cardiovascular diseases, and increased risk of mortality in patients with CKD.
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- 2021
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33. Inhibition of glutamate receptors reduces the homocysteine-induced whole blood platelet aggregation but does not affect superoxide anion generation or platelet membrane fluidization
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Kamil Karolczak, Anna Pieniazek, and Cezary Watala
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excitotoxicity ,glutamate receptors ,homocysteine ,platelet aggregation ,Diseases of the blood and blood-forming organs ,RC633-647.5 - Abstract
Homocysteine (Hcy) is an excitotoxic amino acid. It is potentially possible to prevent Hcy-induced toxicity, including haemostatic impairments, by antagonizing glutaminergic receptors. Using impedance aggregometry with arachidonate and collagen as platelet agonists, we tested whether the blockade of platelet NMDA (N-methyl-D-aspartate), AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) and kainate receptors with their inhibitors: MK-801 (dizocilpine hydrogen maleate, [5R,10S]-[+]-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine), CNQX (7-nitro-2,3-dioxo-1,4-dihydroquinoxaline-6-carbonitrile) and UBP-302 (2-{[3-[(2S)-2-amino-2-carboxyethyl]-2,6-dioxo-3,6-dihydropyrimidin 1(2H)-yl]methyl}benzoic acid) may hamper Hcy-dependent platelet aggregation. All the tested compounds significantly inhibited Hcy-augmented aggregation of blood platelets stimulated either with arachidonate or collagen. Hcy stimulated the generation of superoxide anion in whole blood samples in a concentration-dependent manner; however, this process appeared as independent on ionotropic glutamate receptors, as well as on NADPH oxidase and protein kinase C, and was not apparently associated with the extent of either arachidonate- or collagen-dependent platelet aggregation. Moreover, Hcy acted as a significant fluidizer of surface (more hydrophilic) and inner (more hydrophobic) regions of platelet membrane lipid bilayer, when used at the concentration range from 10 to 50 µmol/l. However, this effect was independent on the Hcy action through glutamate ionotropic receptors, since there was no effects of MK-801, CNQX or UBP-302 on Hcy-mediated membrane fluidization. In conclusion, Hcy-induced changes in whole blood platelet aggregation are mediated through the ionotopic excitotoxic receptors, although the detailed mechanisms underlying such interactions remain to be elucidated.
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- 2017
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34. Genetic variation in Pneumocystis carinii isolates from different geographic regions: implications for transmission.
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Beard, CB, Carter, JL, Keely, SP, Huang, L, Pieniazek, NJ, Moura, IN, Roberts, JM, Hightower, AW, Bens, MS, Freeman, AR, Lee, S, Stringer, JR, Duchin, JS, del Rio, C, Rimland, D, Baughman, RP, Levy, DA, Dietz, VJ, Simon, P, and Navin, TR
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Biomedical and Clinical Sciences ,Clinical Sciences ,Pneumonia ,Genetics ,Pneumonia & Influenza ,Lung ,Infectious Diseases ,Clinical Research ,Infection ,AIDS-Related Opportunistic Infections ,DNA Primers ,Dihydropteroate Synthase ,Gene Frequency ,Genes ,rRNA ,Genetic Variation ,Genotype ,Humans ,Logistic Models ,Mitochondria ,Pneumocystis ,Pneumonia ,Pneumocystis ,RNA ,Ribosomal ,Sequence Analysis ,DNA ,United States ,Medical Microbiology ,Public Health and Health Services ,Microbiology ,Clinical sciences ,Epidemiology ,Health services and systems - Abstract
To study transmission patterns of Pneumocystis carinii pneumonia (PCP) in persons with AIDS, we evaluated P. carinii isolates from patients in five U.S. cities for variation at two independent genetic loci, the mitochondrial large subunit rRNA and dihydropteroate synthase. Fourteen unique multilocus genotypes were observed in 191 isolates that were examined at both loci. Mixed infections, accounting for 17.8% of cases, were associated with primary PCP. Genotype frequency distribution patterns varied by patients' place of diagnosis but not by place of birth. Genetic variation at the two loci suggests three probable characteristics of transmission: that most cases of PCP do not result from infections acquired early in life, that infections are actively acquired from a relatively common source (humans or the environment), and that humans, while not necessarily involved in direct infection of other humans, are nevertheless important in the transmission cycle of P. carinii f. sp. hominis.
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- 2000
35. Prognostic Role of Tumoral PD-L1 and IDO1 Expression, and Intratumoral CD8+ and FoxP3+ Lymphocyte Infiltrates in 132 Primary Cutaneous Merkel Cell Carcinomas
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Piotr Donizy, Cheng-Lin Wu, Janusz Kopczynski, Malgorzata Pieniazek, Przemyslaw Biecek, Janusz Ryś, and Mai P. Hoang
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Merkel cell carcinoma ,PD-L1 ,IDO1 ,CD8 ,FoxP3 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The association of immune markers and clinicopathologic features and patient outcome has not been extensively studied in Merkel cell carcinoma (MCC). We correlated tumoral PD-L1 and IDO1 expression, and intratumoral CD8+ and FoxP3+ lymphocytes count with clinicopathologic variables, Merkel cell polyomavirus (MCPyV) status, and patient outcomes in a series of 132 MCC. By univariate analyses, tumoral PD-L1 expression >1% and combined tumoral PD-L1 >1% and high intratumoral FoxP3+ lymphocyte count correlated with improved overall survival (OS) (p = 0.016, 0.0072), MCC-specific survival (MSS) (p = 0.019, 0.017), and progression-free survival (PFS) (p = 0.043, 0.004, respectively). High intratumoral CD8+ and FoxP3+ lymphocyte count correlated with longer MSS (p = 0.036) and improved PFS (p = 0.047), respectively. Ulceration correlated with worse OS and worse MSS. Age, male gender, and higher stage (3 and 4) significantly correlated with worse survival. MCPyV positivity correlated with immune response. By multivariate analyses, only ulceration and age remained as independent predictors of worse OS; gender and stage remained for shorter PFS. Tumoral PD-L1 expression and increased density of intratumoral CD8+ lymphocytes and FoxP+ lymphocytes may represent favorable prognosticators in a subset of MCCs. Tumoral PD-L1 expression correlated with intratumoral CD8+ and FoxP3+ lymphocytes, which is supportive of an adaptive immune response.
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- 2021
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36. PARP1 as a Marker of an Aggressive Clinical Phenotype in Cutaneous Melanoma—A Clinical and an In Vitro Study
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Piotr Kupczyk, Aleksandra Simiczyjew, Jakub Marczuk, Ewelina Dratkiewicz, Artur Beberok, Jakub Rok, Malgorzata Pieniazek, Przemyslaw Biecek, Dmitry Nevozhay, Bartosz Slowikowski, Grzegorz Chodaczek, Dorota Wrzesniok, Dorota Nowak, and Piotr Donizy
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PARP1 ,cutaneous melanoma ,melanoma cell lines ,immunohistochemistry ,Cytology ,QH573-671 - Abstract
(1) Background: Poly(ADP-ribose) polymerase 1) (PARP1) is a pleiotropic enzyme involved in several cellular processes, e.g., DNA damage repair, regulation of mitosis, and immune response. Little is known about the role of PARP1 in melanoma development and progression. We aimed to investigate the prognostic significance of PARP1 expression in cutaneous melanoma through evaluation of mRNA and protein levels of PARP1 in normal melanocytes and melanoma cell lines, as well as in patients’ tissue material from surgical resections. (2) Methods: An in vitro model was based on two types of normal human melanocytes (HEMn-DP and HEMn-LP) and four melanoma cell lines (A375, WM1341D, Hs294T, and WM9). PARP1 mRNA gene expression was estimated using real-time polymerase chain reaction (RT-PCR), whereas the protein level of PARP1 was evaluated by fluorescence confocal microscopy and then confirmed by Western Blotting analysis. The expression of PARP1 was also assessed by immunohistochemistry in formalin-fixed paraffin-embedded tissues of 128 primary cutaneous melanoma patients and correlated with follow-up and clinicopathologic features. (3) Results: The in vitro study showed that melanoma cells exhibited significantly higher PARP1 expression at mRNA and protein levels than normal melanocytes. High PARP1 expression was also associated with the invasiveness of tumor cells. Elevated nuclear PARP1 expression in patients without nodal metastases strongly correlated with significantly shorter disease-free survival (p = 0.0015) and revealed a trend with shorter cancer-specific overall survival (p = 0.05). High PARP1 immunoreactivity in the lymph node-negative group of patients was significantly associated with higher Breslow tumor thickness, presence of ulceration, and a higher mitotic index (p = 0.0016, p = 0.023, and p < 0.001, respectively). In patients with nodal metastases, high PARP1 expression significantly correlated with the presence of microsatellitosis (p = 0.034), but we did not confirm the prognostic significance of PARP1 expression in these patients. In the entire analyzed group of patients (with and without nodal metastases at the time of diagnosis), PARP1 expression was associated with a high mitotic index (p = 0.001) and the presence of ulceration (p = 0.036). Moreover, in patients with elevated PARP1 expression, melanoma was more frequently located in the skin of the head and neck region (p = 0.015). In multivariate analysis, high PARP1 expression was an independent unfavorable prognosticator in lymph node-negative cutaneous melanoma patients. (4) Conclusions: In vitro molecular biology approaches demonstrated enhanced PARP1 expression in cutaneous melanoma. These results were confirmed by the immunohistochemical study with clinical parameter analysis, which showed that a high level of PARP1 correlated with unfavorable clinical outcome. These observations raise the potential role of PARP1 inhibitor-based therapy in cutaneous melanoma.
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- 2021
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37. Empagliflozin in patients admitted to hospital with COVID-19 (RECOVERY): a randomised, controlled, open-label, platform trial
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Abani, O, Abbas, A, Abbas, F, Abbas, J, Abbas, K, Abbas, M, Abbasi, S, Abbass, H, Abbott, A, Abdallah, N, Abdelaziz, A, Abdelfattah, M, Abdelqader, B, Abdul, A, Abdul, B, Abdul, S, Abdul Rasheed, A, Abdulakeem, A, Abdul-Kadir, R, Abdullah, A, Abdulmumeen, A, Abdul-Raheem, R, Abdulshukkoor, N, Abdusamad, K, Abed El Khaleq, Y, Abedalla, M, Abeer Ul Amna, A, Abel, L, Abernethy, K, Abeywickrema, M, Abhinaya, C, Abidin, A, Aboaba, A, Aboagye-Odei, A, Aboah, C, Aboelela, H, Abo-Leyah, H, Abouelela, K, Abou-Haggar, A, Abouibrahim, M, Abousamra, A, Abouzaid, M, Abraham, M, Abraham, T, Abraheem, A, Abrams, J, Abrams, R, Abu, HJ, Abu-Arafeh, A, Abubacker, SM, Abung, A, Abusamra, Y, Aceampong, Y, Achara, A, Acharya, D, Acheampong, F, Acheampong, P, Acheampong, S, Acheson, J, Achieng, S, Acosta, A, Acquah, R, Acton, C, Adabie-Ankrah, J, Adair, P, Adam, AS, Adam, F, Adam, M, Adamali, H, Adamczyk, M, Adams, C, Adams, D, Adams, K, Adams, L, Adams, N, Adams, R, Adams, T, Adamu-Ikeme, L, Adatia, K, Adcock, K, Addai-Boampong, L, Addo, A, Adeagbo, O, Adebiyi, A, Adedeji, O, Adegeye, Y, Adegoke, K, Adell, V, Adenwalla, S, Adeoye, FW, Adesemoye, OA, Adewunmi, EO, Adeyanju, A, Adeyemi, J, Adeyemo, T, Adhikari, B, Adhikari, SA, Adhikary, R, Aditya, A, Adjepong, P, Adkins, G, Adnan, A, Adriaanse, M, Aeron-Thomas, J, Affleck, D, Afnan, C, Afridi, M, Afrim, P, Afriyie, FA, Aftab, ZA, Afum-Adjei Awuah, A, Agarwal, M, Agasiya, PN, Agbeko, R, Agbo, C, Aggarwal, S, Aghababaie, A, Aguilar Jimenez, L, Agyekum, JA, Agyen, K, Ahadome, EK, Ahamed Sadiq, S, Ahammed Nazeer, MH, Ahmad, M, Ahmad, S, Ahmed, A, Ahmed, BAR, Ahmed, B, Ahmed, F, Ahmed, H, Ahmed, I, Ahmed, K, Ahmed, L, Ahmed, M, Ahmed, MC, Ahmed, MS, Ahmed, N, Ahmed, N, Ahmed, O, Ahmed, RA, Ahmed, R, Ahmed, R, Ahmed, S, Ahmed, SG, Ahmed, SH, Ahmed Ali, R, Ahmed Mohamud, B, Ahmed, S, Ahmer, S, Ahonia, A, Aidoo, C, Aiken, C, Ail, D, Ainsworth, M, Aissa, M, Aitken, L, Ajay, B, Ajibode, A, Ajmi, A, Akhtar, N, Akili, S, Akinbiyi, B, Akindolie, O, Akinfenwa, Y, Akinkugbe, O, Akinpelu, I, Akram, M, Aktinade, O, Akudi, U, Al Aaraj, ASAR, Al Balushi, A, Al Dakhola, M, Al Swaifi, A, Al-Abadi, E, Alabi, A, Aladangady, N, Alafifi, M, Alam, A, Alam, S, Al-Asadi, A, Alatzoglou, K, Albert, P, Albertus, A, Albon, L, Alcala, A, Alcorn, G, Alcorn, S, Aldana, A, Alderdice, D, Aldesouki, A, Aldouri, R, Aldridge, J, Aldridge, N, Ale, RM, Alegria, A, Alexander, A, Alexander, C, Alexander, J, Alexander, PDG, Al-fori, J, Alghazawi, L, Alhabsha, O, Al-Hakim, B, Alhameed, R, Al-Hayali, M, Al-Hity, S, Ali, A, Ali, FR, Ali, J, Ali, M, Ali, MS, Ali, N, Ali, O, Ali, R, Ali, S, Aliberti, E, Alin, J, Alina, A, Alipustain, A, Alisjahbana, B, Aliyuda, F, Alizadeh, K, Al-Jibury, M, Al-Juboori, S, Al-Khalil, M, Alkhudhayri, A, Alkhusheh, M, Allan, F, Allan, N, Allanson, A, Allcock, R, Allen, E, Allen, J, Allen, K, Allen, L, Allen, P, Allen, R, Allen, S, Allen, T, Alli, A, Allison, K, Allman, B, Allsop, HK, Allsop, L, Allsup, D, Almahroos, AFT, Al-Moasseb, H, Al-Obaidi, M, Alomari, L, Al-Rabahi, A, Al-Ramadhani, B, Al-Saadi, Z, Al-Sammarraie, R, Alshaer, I, Al-Shahi Salman, R, Al-Shamkhani, W, Alsheikh, F, Al-Sheklly, B, Altaf, S, Alty, A, Alvarez, M, Alvarez Corral, M, Alveyn, E, Alzetani, M, Amamou, S, Amar, N, Ambalavanan, S, Ambrogetti, R, Ambrose, C, Ameen, A, Amelia Ganefianty, A, Ames, K, Amezaga, MR, Amin, A, Amin, A, Amin, K, Amin, S, Amin, T, Amit, B, Amjad, A, Amjad, N, Amoah-Dankwa, J, Amoako-Adusei, A, Amosun, V, Amsal, M, Amsha, K, Amuasi, J, Amutio Martin, N, Amy, P, Anada, A, Anand, A, Anandappa, S, Anantapatnaikuni, SD, Andari, NKN, Anderson, E, Anderson, J, Anderson, L, Anderson, M, Anderson, N, Anderson, R, Anderson, S, Anderson, W, Andreou, P, Andrews, A, Andrews, J, Aneke, K, Ang, A, Ang, WW, Angel, T, Angela, A, Angelini, P, Anguvaa, L, Anichtchik, O, Anim-Somuah, M, Aniruddhan, K, Annett, J, Anning, L, Ansah, M, Anstey, PJ, Anstey, R, Anthony, A, Anthony-Pillai, A, Antill, P, Antonina, Z, Anu, V, Anwar, M, Anwar, S, Apetri, E, Apostolopoulos, A, Appleby, S, Appleyard, D, Aquino, MF, Araba, B, Aransiola, S, Araujo, M, Archer, A, Archer, D, Archer, S, Arcoria, D, Ardley, C, Arhin-Sam, G, Arias, A-M, Aribike, O, Arimoto, R, Arisanti, NLPE, Arkley, C, Armah, C, Armata, I, Armistead, J, Armitage, A, Armstrong, C, Armstrong, M, Armstrong, S, Armstrong, W, Armtrong, P, Arndt, H, Arnison-Newgass, C, Arnold, D, Arnold, R, Arnott, A, Arora, D, Arora, K, Arora, P, Arora, R, Arter, A, Arthur, A, Artini, NM, Arumaithurai, A, Arya, A, Arya, R, Aryal, D, Asandei, D, Asare, GA, Asghar, A, Asghar, M, Ashab, A, Ashbrook-Raby, C, Ashby, H, Ashcroft, J, Ashcroft, S, Asher, G, Ashfak, Z, Ashfaq, A, Asiamah, HA, Ashish, A, Ashley, D, Ashman-Flavell, S, Ashok, S, Ashour, A-E-A, Ashraf, MZ, Ashraf, S, Ashraq, MB, Ashton, D, Ashton, S, Ashworth, A, Ashworth, FJ, Ashworth, R, Aslam, A, Aslam, I, Aslam, S, Aslett, L, Asogan, H, Asrar, A, Assaf, O, Astin-Chamberlain, R, Atabudzi, YE, Athavale, P, Athorne, D, Atkins, B, Atkins, C, Atkins, S, Atkinson, J, Atkinson, V, Atomode, A, Atraskiewicz, B, Attia, AA, Attubato, E, Attwood, M, Aubrey, P, Auer, Z, Aujayeb, A, Aung, AT, Aung, H, Aung, HWW, Aung, KK, Aung, KT, Aung, N, Aung, Y, Aung, ZM, Austin, E, Austin, K, Auwal, A, Avari, M, Avery, M, Aveyard, N, Avis, J, Aviss, G, Avram, C, Avram, P, Awadelkareem, A, Awadzi, G, Awaly, M, Awan, A, Awisi, S, Aya, A, Ayaz, E, Ayerh, JM, Ayers, A, Azam, J, Azeem, A, Azharuddin, M, Aziz, A, Aziz, G, Aziz, I, Aziz, N, Azkoul, A, Azman Shah, A, Azzopardi, G, Azzoug, H, Babatunde, F, Babi, M, Babiker, B, Babington, G, Babirecki, M, Babores, M, Babs-Osibodu, AO, Bac, T, Bacciarelli, S, Bachar, R, Bachour, M-E, Bachti, A, Bacon, G, Bacon, J, Badal, B, Badat, A, Bader, M, Badhan, GR, Badhrinarayanan, S, Bae, JP, Baggaley, A, Baggott, A, Bagley, G, Bagmane, D, Bagshaw, L, Bahadori, K, Bahurupi, Y, Bailey, A, Bailey, J, Bailey, K, Bailey, L, Bailey, MA, Bailey, M, Bailey, P, Bailey, S, Baillie, H, Baillie, JK, Bain, J, Bains, V, Baird, D, Baird, E, Baird, K, Baird, S, Baird, T, Baird, Y, Bajandouh, A, Baker, DC, Baker, E, Baker, J, Baker, K, Baker, M, Baker, R, Baker, T-A, Baker, V, Bakere, H, Bakerly, N, Baker-Moffatt, M, Bakhai, A, Bakhtiar, N, Bakoulas, P, Bakthavatsalam, D, Balachandran, N, Balan, A, Balasingam, P, Balaskas, T, Balasubramaniam, M, Balatoni, N, Balcombe, A, Baldwin, A, Baldwin, A, Baldwin, C, Baldwin, D, Baldwin, F, Baldwin-Jones, R, Bale, N, Balfour, J, Ball, M, Ball, Ro, Ballard, K, Balluz, I, Balmforth, C, Balogh, E, Baltmr, A, Baluwala, A, Bambridge, G, Bamford, A, Bamford, P, Bamgboye, A, Bancroft, E, Bancroft, H, Banda, J, Bandaru, K, Bandi, S, Bandla, N, Bandyopadhyam, S, Banerjee, A, Banerjee, R, Bang, P, Baniya, S, Bani-Saad, O, Banks, H, Banks, L, Banks, P, Bann, C, Bannister, H, Bannister, O, Banton, L, Bao, T, Baptist, M, Baqai, T, Baral, AM, Baral, SC, Baramova, D, Barber, R, Barbon, E, Barbosa, M, Barbour, J, Barclay, A, Barclay, C, Bardsley, G, Bareford, S, Bari, S, Barimbing, M, Barker, A, Barker, D, Barker, E, Barker, H, Barker, J, Barker, L, Barker, O, Barker-Williams, K, Barkha, S, Barla, J, Barlow, G, Barlow, R, Barlow, V, Barnacle, J, Barnard, A, Barnes, D, Barnes, N, Barnes, R, Barnes, T, Barnetson, C, Barnett, A, Barnett-Vanes, A, Barning, PG, Barnsley, W, Barr, A, Barr, D, Barr, J, Barr, C, Barratt, N, Barratt, S, Barrera, M, Barrett, A, Barrett, Fi, Barrett, J, Barrett, S, Barrow, E, Bartholomew, J, Barthwal, MS, Bartlett, C, Bartlett, G, Bartlett, J, Bartlett, L, Bartley, S, Bartolmeu-Pires, S, Barton, A, Barton, G, Barton, J, Barton, L, Barton, R, Baruah, R, Baryschpolec, S, Bashir, H, Bashyal, A, Basker, B, Basnet, S, Basnyat, B, Basoglu, A, Basran, A, Bassett, J, Bassett, G, Bassford, C, Bassoy, B, Bastion, V, Bastola, A, Basumatary, A, Basvi, P, Batac, JA, Bataduwaarachchi, VR, Bate, T, Bateman, HJ, Bateman, K, Bateman, V, Bates, E, Bates, H, Bates, M, Bates, S, Batham, S, Batista, A, Batla, A, Batra, D, Batty, H, Batty, T, Batty, A, Baum, M, Baumber, R, Bautista, C, Bawa, F, Bawa, T, Bawani, FS, Bax, S, Baxter, M, Baxter, N, Baxter, Z, Bayes, H, Bayo, L-A, Bazari, F, Bazaz, R, Bazli, A, Beacham, L, Beadles, W, Beadon, K, Beak, P, Beale, A, Beard, K, Bearpark, J, Beasley, A, Beattie, S, Beaumont, K, Beaumont-Jewell, D, Beaver, T, Beavis, S, Beazley, C, Beck, S, Beckett, V, Beckitt, R, Beckley, S, Beddall, H, Beddows, S, Beeby, D, Beeby, S, Beech, G, Beecroft, M, Beer, N, Beer, Sa, Beety, J, Bega, G, Begg, A, Begg, S, Beghini, S, Begum, A, Begum, S, Begum, S, Behan, T, Behrouzi, R, Beishon, J, Beith, C, Belcher, J, Belfield, H, Belfield, K, Belgaumkar, A, Bell, D, Bell, G, Bell, J, Bell, L, Bell, N, Bell, P, Bell, S, Bellamu, J, Bellamy, M, Bellamy, T, Bellini, A, Bellis, A, Bellis, F, Bendall, L, Benesh, N, Benetti, N, Bengu, SA, Benham, L, Benison-Horner, G, Benkenstein, S, Benn, T, Bennett, A, Bennett, C, Bennett, D, Bennett, G, Bennett, K, Bennett, K, Bennett, L, Bennett, MR, Bennett, S, Bennion, K, Benoy, G, Benson, V, Bentley, A, Bentley, J, Benton, I, Beranova, E, Beresford, M, Bergin, C, Bergstrom, M, Bernatoniene, J, Berriman, T, Berry, Z, Best, F, Best, K, Bester, A-M, Beuvink, Y, Bevan, E, Bevins, S, Bewick, T, Bexley, A, Beyatli, S, Beynon, F, Bhadi, A, Bhagani, S, Bhakta, S, Bhalla, R, Bhandal, K, Bhandal, K, Bhandari, A, Bhandari, LN, Bhandari, S, Bhanich Supapol, J, Bhanot, A, Bhanot, R, Bhasin, S, Bhat, A, Bhat, P, Bhatnagar, R, Bhatt, K, Bhayani, J, Bhojwani, D, Bhuie, P, Bhuiyan, MS, Bhuiyan, S, Bibby, A, Bibi, F, Bibi, N, Bibi, S, Bicanic, T, Bidgood, S, Bigg, J, Biggs, S, Biju, A, Bikov, A, Billingham, S, Billings, J, Binh, P, Binns, A, BinRofaie, M, Bintcliffe, O, Birch, C, Birch, J, Birchall, K, Bird, S, Birt, M, Bishop, C, Bishop, K, Bishop, L, Bisnauthsing, K, Biswas, N, Bittaye, M, Biuk, S, Blachford, K, Black, E, Black, H, Black, K, Black, M, Black, P, Black, V, Blackgrove, H, Blackledge, B, Blackler, J, Blackley, S, Blackman, H, Blackstock, C, Blair, C, Blakemore, F, Blamey, H, Bland, A, Blane, S, Blankley, S, Blaxill, P, Blaylock, K, Blazeby, J, Blencowe, N, Bloom, B, Bloomfield, J, Bloss, A, Blowers, A, Blows, S, Bloxham, H, Blrd, S, Blundell, L, Blunsum, A, Blunt, M, Blunt, T, Blyth, I, Blyth, K, Blythe, A, Blythe, K, Boahen, KA, Boampoaa, M, Board, S, Boatemah, E, Bobie, B, Bobruk, K, Bodalia, PN, Bodasing, N, Boden, M, Bodenham, T, Boehmer, G, Boffito, M, Bohanan, K, Bohmova, K, Bohnacker, N, Bokhandi, S, Bokhar, M, Bokhari, S, Bokhari, SO, Bokobza, I, Boles, A, Bolger, C, Bonaconsa, C, Bond, C, Bond, H, Bond, S, Bond, T, Bone, A, Boniface, G, Bonney, J, Bonney, L, Booker, L, Boot, S, Boothroyd, M, Borbone, J, Borman, N, Bosence, S, Bostock, K, Botting, N, Bottrill, F, Bouattia, H, Bough, L, Boughton, H, Boult, Z, Boumrah, T, Bourke, M, Bourke, S, Bourne, M, Bousfield, R, Boustred, L, Bowes, A, Bowker, P, Bowker, T, Bowler, H, Bowman, L, Bowman, S, Bowmer, R, Bowring, A, Bowyer, H, Boyd, A, Boyd, J, Boyd, L, Boyer, N, Boyle, N, Boyle, P, Boyle, R, Boyles, L, Brace, L, Bracken, A, Bradder, J, Bradley, CJ, Bradley, P, Bradley-Potts, J, Bradshaw, L, Bradshaw, Z, Brady, C, Brady, R, Brady, S, Braga Sardo, P, Braganza, D, Braithwaite, M, Brammer, S, Branch, M, Brankin-Frisby, T, Brannigan, J, Brattan, S, Bray, F, Bray, N, Brazil, M, Brear, L, Brear, Tr, Brearey, S, Bremner, L, Brend, M, Bresges, C, Bressington, C, Bretland, G, Brewer, C, Bridgett, M, Bridgwood, G, Brigham, S, Bright, J, Brightling, C, Brigstock, T, Brimfield, L, Brinksman, P, Brinkworth, E, Brittain-Long, R, Britten, V, Britton, H, Broad, L, Broadhead, S, Broadhurst, R, Broadley, A, Broadway, M, Brockelsby, C, Brocken, M, Brockley, T, Brodsky, M, Brogan, F, Brohan, L, Brokke, F, Brolly, J, Bromley, D, Brooke-Ball, H, Brooker, V, Brookes, M, Brooking, D, Brooks, A, Brooks, D, Brooks, J, Brooks, K, Brooks, N, Brooks, P, Brooks, R, Brooks, S, Broom, M, Broomhead, N, Broughton, C, Broughton, N, Brouns, M, Brown, A, Brown, C, Brown, E, Brown, H, Brown, J, Brown, L, Brown, N, Brown, P, Brown, R, Brown, S, Brown, T, Browne, B, Browne, C, Browne, D, Browne, M, Brownlee, S, Brraka, A, Bruce, J, Bruce, M, Brudlo, W, Brunchi, A, Brunskill, N, Brunton, A, Brunton, M, Bryant, M, Bryden, E, Brzezicki, H, Buazon, A, Buch, MH, Buchan, R, Buchanan, R, Buche, D, Buck, A, Buck, L, Buckland, M, Buckley, C, Buckley, L, Buckley, P, Buckley, S, Buckman, C, Budds, A, Bugg, G, Bujazia, R, Bukhari, M, Bukhari, S, Bulbulia, R, Bull, A, Bull, D, Bull, K, Bull, R, Bull, Th, Bullock, E, Bullock, S, Bulteel, N, Bumunarachchi, K, Bungue-Tuble, R, Burbidge, O, Burchett, C, Burda, D, Burden, C, Burden, TG, Burgess, Mi, Burgess, R, Burgess, S, Burhan, E, Burhan, H, Burke, H, Burke, K, Burman, A, Burnard, S, Burnett, C, Burnett, S, Burns, A, Burns, C, Burns, J, Burns, K, Burrage, D, Burrows, K, Burston, C, Burton, A, Burton, B, Burton, F, Burton, H, Burton, M, Butar butar, M, Butcher, D, Butler, A, Butler, E, Butler, J, Butler, P, Butler, S, Butler, J, Butt, A-T, Butt, M, Butt, MM, Butterworth, C, Butterworth-Cowin, N, Buttery, R, Buttle, T, Button, H, Buttress, D, Bye, H, Byrne, J, Byrne, W, Byrne-Watts, V, Cabandugama, A, Cabrero, L, Caddy, S, Cade, R, Cadwgan, A, Cahilog, Z, Cahyareny, A, Cairney, D, Calderwood, J, Caldow, D, Cale, E, Calisti, G, Callaghan, D, Callaghan, J, Callens, C, Callum, D, Calver, C, Cambell-Kelly, M, Camburn, T, Cameron, DR, Cameron, E, Cameron, F, Cameron, S, Camm, C, Cammack, FD, Campbell, A, Campbell, B, Campbell, D, Campbell, H, Campbell, J, Campbell, K, Campbell, M, Campbell, R, Campbell, W, Campbell Hewson, Q, Camsooksai, J, Canclini, L, Candido, SM, Candlish, J, Caneja, C, Cann, A, Cann, J, Cannan, R, Cannon, A, Cannon, E, Cannon, M, Cannon, P, Cannons, V, Canonizado, E, Cantliff, J, Cap, N, Caplin, B, Capocci, S, Caponi, N, Capp, A, Capstick, R, Capstick, T, Caraenache, C, Card, A, Cardwell, M, Carey, C, Carey, R, Carley, S, Carlin, F, Carlin, T, Carmichael, S, Carmody, M, Carnahan, M, Caroline, C, Carpenter, J, Carr, S, Carrasco, A, Carrington, Z, Carroll, A, Carroll, P, Carson, R, Cart, C, Carter, E, Carter, J, Carter, M, Carter, N, Carter, P, Cartwright, D, Cartwright, J-A, Carty, C, Carty, L, Carungcong, J, Carver, C, Carver, E, Carver, R, Casey, S, Cassells, A, Castiello, T, Castle, G, Castles, B, Caswell, M, Catana, AM, Cate, H, Catelan Zborowski, A, Cathcart, S, Cathie, K, Catibog, D, Catley, C, Catlow, L, Caudwell, M, Cavazza, A, Cave, A, Cave, L, Cavinato, S, Cawa, F, Cawley, K, Caws, C, Cawthorne, K, Cendl, H, Century, H, Cernova, J, Cesay, M, Cetti, E, Chabane, S, Chablani, M, Chabo, C, Chacko, J, Chadwick, D, Chadwick, J, Chadwick, R, Chakkarapani, E, Chakraborty, A, Chakraborty, M, Chakravorty, M, Chalakova, P, Chalise, BS, Chalmers, J, Chalmers, R, Chamberlain, G, Chamberlain, S, Chambers, E, Chambers, J, Chambers, L, Chambers, N, Chan, A, Chan, C, Chan, E, Chan, M, Chan, K, Chan, P, Chan, R (P-C), Chan, XHS, Chandler, C, Chandler, H, Chandler, KJ, Chandler, S, Chandler, Z, Chandra, S, Chandran, N, Chandrasekaran, B, Chang, Y, Chanh, HQ, Chaplin, G, Chaplin, J, Chapman, G, Chapman, J, Chapman, K, Chapman, L, Chapman, M, Chapman, P, Chapman, T, Chappell, L, Charalambou, A, Charles, B, Charlton, D, Charlton, S, Chatar, K, Chatha, C, Chatterton, D, Chau, N, Chaube, R, Chaudhary, MYN, Chaudhary, B, Chaudhry, I, Chaudhry, Z, Chaudhuri, K, Chaudhuri, N, Chaudhury, M, Chauhan, A, Chauhan, RS, Chavasse, A, Chavasse, N, Chawla, V, Cheater, L, Cheaveau, J, Cheeld, C, Cheeseman, M, Chen, F, Chen, HM, Chen, T, Cheng, F, Cheng, LY, Cheng, Z, Chenoweth, H, Cheong, CH, Cherian, JJ, Cherian, S, Cherrie, M, Cheshire, H, Cheung, CK, Cheung, E, Cheung, K, Cheung, M, Cheyne, C, Chhabra, S, Chia, WL, Chiang, E, Chiapparino, A, Chicano, R, Chikara, G, Chikungwa, M, Chikwanha, ZA, Chilcott, G, Chilcott, S, Chilvers, A, Chimbo, P, Chin, KW, Chin, WJ, Chineka, R, Chingale, A, Chinonso, E, Chin-Saad, C, Chirgwin, M, Chisem, H, Chisenga, C, Chisholm, C, Chisnall, B, Chiswick, C, Chita, S, Chitalia, N, Chiu, M, Chiverton, L, Chivima, B, Chmiel, C, Choi, S, Choon Kon Yune, W, Chopra, M, Choudhary, V, Choudhury, O, Choudhury, S, Chow, B-L, Chowdhury, M, Chowdhury, S, Chrisopoulou, A, Christenssen, V, Christian, P, Christides, A, Christie, F, Christmas, D, Christoforou, G, Christopherson, T, Christou, A, Christy, M, Chrysostomou, P, Chua, Y, Chudgar, D, Chudleigh, R, Chukkambotla, S, Chukwu, ME, Chukwulobelu, I, Chung, CY, Church, E, Church, SR, Churchill, D, Cianci, N, Cicconi, P, Cinardo, P, Cipinova, Z, Cipriano, B, Clamp, S, Clancy, B, Clapham, M, Clare, E, Clare, S, Clark, A, Clark, C, Clark, D, Clark, E, Clark, F, Clark, G, Clark, J, Clark, K, Clark, L, Clark, M, Clark, N, Clark, P, Clark, R, Clark, T, Clark, Z, Clarke, A, Clarke, J, Clarke, P, Clarke, R, Clarke, S, Claxton, A, Claxton, L, Clay, K, Clayton, C, Clayton, E, Clayton, O, Clayton-Smith, J, Clearyb, B, Cleaver, C, Cleeton, R, Clement, I, Clemente de la Torre, C, Clements, J, Clements, S, Clenton, S, Cliff, S, Clifford, R, Clifford, S, Clive, A, Clouston, J, Clubb, V, Clueit, S, Clutterbuck, L, Clyne, A, Coakley, M, Coakley, PGL, Cobain, K, Cochrane, A, Cochrane, P, Cockayne, L, Cockerell, M, Cockerill, H, Cocks, S, Codling, R, Coe, A, Coetzee, S, Coey, D, Cohen, D, Cohen, J, Cohen, O, Cohn, M, Coke, L, Coker, O, Colbeck, N, Colbert, R, Cole, E, Cole, J, Coleman, G, Coleman, M, Coleman, N, Coles, H, Colin, M, Colino-Acevedo, A, Colley, J, Collie, K, Collier, A, Collier, D, Collier, H, Collingwood, T, Collini, P, Collins, E, Collins, J, Collins, K, Collins, M, Collins, N, Collins, S, Collins, V, Collinson, A, Collinson, B, Collinson, J, Collis, M, Colmar, M, Colton, HE, Colton, J, Colville, K, Colvin, C, Combes, E, Comer, D, Comerford, A, Concannon, D, Condliffe, A, Condliffe, R, Connell, E, Connell, L, Connell, N, Connelly, K, Connolly, G, Connor, E, Conroy, A, Conroy, K, Conteh, V, Convery, R, Conway, F, Conway, G, Conway, R, Conyngham, J-A, Cook, A, Cook, C, Cook, E, Cook, G, Cook, H, 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- Abstract
Empagliflozin has been proposed as a treatment for COVID-19 on the basis of its anti-inflammatory, metabolic, and haemodynamic effects. The RECOVERY trial aimed to assess its safety and efficacy in patients admitted to hospital with COVID-19.
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- 2023
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38. DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: a short-term supplementation trial with respect to GPX1 and SEPP1 polymorphism
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Jablonska, E., Raimondi, S., Gromadzinska, J., Reszka, E., Wieczorek, E., Krol, M. B., Smok-Pieniazek, A., Nocun, M., Stepnik, M., Socha, K., Borawska, M. H., and Wasowicz, W.
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- 2016
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39. Alterations in conformational state of albumin in plasma in chronic hemodialyzed patients.
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Anna Pieniazek, Lukasz Gwozdzinski, Zbigniew Zbrog, and Krzysztof Gwozdzinski
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Medicine ,Science - Abstract
In chronic hemodialyzed (CH) patients the balance between production of reactive oxygen species and antioxidant defense system is disturbed and shifted towards oxidative conditions. The properties of albumin in CH patients were studied before hemodialysis (HD) and post-HD.Two oxidants were applied, organic t-butyl hydroperoxide (t-BOOH) and inorganic hydroperoxide (H2O2), for oxidation of albumin molecules. By comparison, albumin from healthy donors was also modified by both oxidants. The thiol content in albumin was determined by the Ellman method. Albumin properties were evaluated with the spin labelling technique using two covalently bound spin labels, maleimide (MSL) and iodoacetamide (ISL), and fatty acid spin probe, 16-doxylstearic acid (16-DS).A decrease in thiols level in HD albumin was greater than in control albumin. The t-BOOH modified the microenvironment at the binding site of MSL and ISL in control albumin molecules to a greater extent than hydrogen peroxide. Control albumin treated with t-BOOH and H2O2 showed an increase in the mobility of 16-DS. However, no changes were observed in albumin from CH patients treated with either of the oxidizing agents.Both oxidants induced strong conformational changes in albumin from healthy volunteers, but were less effective or ineffective in modification of albumin derived from CH patients. These results show that albumin from CH patients is highly modified in vivo and is not vulnerable to oxidation in the same way as normal albumin.
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- 2018
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40. Dysregulation of markers of oxidative stress and DNA damage among nail technicians despite low exposure to volatile organic compounds
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Peter Grešner, Maciej Stepnik, Magdalena Beata Król, Radoslaw Swiercz, Anna Smok-Pieniazek, Ewa Twardowska, Jolanta Gromadzińska, and Wojciech Wasowicz
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occupational exposure ,exposure ,dna damage ,biomarker ,volatile organic compound ,dna ,dysregulation ,marker of oxidative stress ,nail technician ,voc ,Public aspects of medicine ,RA1-1270 - Abstract
OBJECTIVE: The study aimed to compare levels of selected biomarkers of oxidative stress and DNA damage and their correlation with occupational exposure to volatile organic compounds (VOC) among female nail technicians and a group of unexposed volunteers. METHODS: A panel of biomarkers of oxidative stress and DNA damage was assayed among 145 female nail technicians and 152 healthy female volunteers. Occupational exposure of nail technicians to VOC was assessed analyzing the VOC content in nail salon air samples. RESULTS: The level of occupational exposure of nail technicians to VOC was below the respective threshold limit values with combined airborne exposure to a mixture of VOC, reaching only 3.3% (range 0.2–33.3%) of the threshold limit. Despite that, nail technicians presented increased activity of glutathione peroxidase 1 (GPx1), plasma ceruloplasmin, and the GPx1/superoxide dismutase 1 ratio (P
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- 2015
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41. FREQUENCY OF CERVICAL AND INTRACRANIAL ARTERIES LESIONS AND ASSOCIATED CLINICAL SYMPTOMS IN PATIENTS WITH CONFIRMED RENAL FIBROMUSCULAR DYSPLASIA - ARCADIA-POL STUDY
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Swiatlowski, L., Warchol-Celinska, E., Kowalczyk, K., Januszewicz, M., Florczak, E., Prejbisz, A., Jozwik-Plebanek, K., Michalowska, I., Witowicz, H., Kurkowska-Jastrzebska, I., Adamczak, M., Pajak, I., Kabat, M., Odrowaz-Pieniazek, P., Tekieli, L., Wozakowska-Kaplon, B., Stefanczyk, L., Przewlocki, T., Januszewicz, A., and Szczerbo-Trojanowska, M.
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- 2018
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42. Applying organizational psychology as a design science: A method for predicting malfunctions in socio-technical systems (PreMiSTS)
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Chris W. Clegg, Mark A. Robinson, Matthew C. Davis, Lucy E. Bolton, Rebecca L. Pieniazek, and Alison McKay
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prediction ,socio-technical systems ,malfunctions ,accidents ,big data ,Drawing. Design. Illustration ,NC1-1940 ,Engineering design ,TA174 - Abstract
As a discipline, design science has traditionally focused on designing products and associated technical processes to improve usability and performance. Although significant progress has been made in these areas, little research has yet examined the role of human behaviour in the design of socio-technical systems (e.g., organizations). Here, we argue that applying organizational psychology as a design science can address this omission and enhance the capability of both disciplines. Specifically, we propose a method to predict malfunctions in socio-technical systems (PreMiSTS), thereby enabling them to be designed out or mitigated. We introduce this method, describe its nine stages, and illustrate its application with reference to two high-profile case studies of such malfunctions: (1) the severe breakdowns in patient care at the UK’s Mid-Staffordshire NHS Foundation Trust hospital in the period 2005–2009, and (2) the fatal Grayrigg rail accident in Cumbria, UK, in 2007. Having first identified the socio-technical and behavioural antecedents of these malfunctions, we then consider how the PreMiSTS method could be used to predict and prevent future malfunctions of this nature. Finally, we evaluate the method, consider its advantages and disadvantages, and suggest where it can be most usefully applied.
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- 2017
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43. The nucleotide excision repair protein XPC is essential for bulky DNA adducts to promote interleukin-6 expression via the activation of p38-SAPK
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Schreck, I, Grico, N, Hansjosten, I, Marquardt, C, Bormann, S, Seidel, A, Kvietkova, D L, Pieniazek, D, Segerbäck, D, Diabaté, S, van der Horst, G T J, Oesch-Bartlomowicz, B, Oesch, F, and Weiss, C
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- 2016
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44. Tubulinosema sp. Microsporidian Myositis in Immunosuppressed Patient
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Maria M. Choudhary, Maureen G. Metcalfe, Kathryn Arrambide, Caryn Bern, Govinda S. Visvesvara, Norman J. Pieniazek, Rebecca D. Bandea, Marlene DeLeon-Carnes, Patricia Adem, Moaz M. Choudhary, Sherif R. Zaki, and Musab U. Saeed
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fungal infections ,microsporidia ,Tubulinosema ,immunosuppression ,myositis ,dispatch ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
The Phylum Microsporidia comprises >1,200 species, only 15 of which are known to infect humans, including the genera Trachipleistophora, Pleistophora, and Brachiola. We report an infection by Tubulinosema sp. in an immunosuppressed patient.
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- 2011
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45. Prevalence of intestinal microsporidiosis in Human Immunodeficiency Virus-infected patients with diarrhea in major United States cities Prevalência de microsporidiose intestinal em pacientes infectados pelo HIV com diarréia nas principais cidades dos Estados Unidos da América do Norte
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Mark S. Dworkin, Susan E. Buskin, Arthur J. Davidson, David L. Cohn, Anne Morse, Jeffrey Inungu, Michael R. Adams, Scott B. McCombs, Jeffrey L. Jones, Hercules Moura, Govinda Visvesvara, Norman J. Pieniazek, and Thomas R. Navin
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Intestinal microsporidiosis ,Prevalence ,HIV-infected patients ,Diarrhea ,Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
To determine the prevalence of intestinal microsporidiosis in HIV-infected patients, we performed a prospective study of HIV-infected patients with diarrheal illnesses in three US hospitals and examined an observational database of HIV-infected patients in 10 US cities. Among 737 specimens from the three hospitals, results were positive for 11 (prevalence 1.5%); seven (64%) acquired HIV through male-to-male sexual contact, two (18%) through male-to-male sexual contact and injection drug use, and one (9%) through heterosexual contact; one (9%) had an undetermined mode of transmission. Median CD4 count within six months of diagnosis of microsporidiosis was 33 cells/µL (range 3 to 319 cells/µL). For the national observational database (n = 24,098), the overall prevalence of microsporidiosis was 0.16%. Prevalence of microsporidiosis among HIV-infected patients with diarrheal disease is low, and microsporidiosis is most often diagnosed in patients with very low CD4+ cell counts. Testing for microsporidia appears to be indicated, especially for patients with very low CD4+ cell counts.Para determinar a prevalência de microsporidiose intestinal em pacientes infectados pelo HIV foi realizado um estudo prospectivo em três hospitais dos Estados Unidos da América do Norte (EUA) e analizada uma base de dados nacional composta de dados coletados de pacientes infectados pelo HIV em 10 cidades dos EUA. De um total de 737 amostras de fezes de pacientes infectados pelo HIV que apresentavam diarréia, amostras de 11 pacientes (prevalência de 1,5%) foram positivas para microsporídios. Todos os positivos eram do sexo masculino e, entre eles, sete (64%) pacientes adquiriram a infecção pelo HIV através de relação homossexual, dois (18%) através de relação sexual e drogas injetáveis e um (9%) através de contato heterosexual, enquanto que em um paciente o modo de transmissão do HIV não foi determinado. A contagem média de linfócitos CD4 realizada até seis meses do diagnóstico de microsporidiose foi de 33 células/microlitro (3 a 319 células/microlitro). A análise da base de dados nacional (n = 24.098) mostrou uma prevalência de microsporidiose de 0,16%. A prevalência de microsporidiose em pacientes HIV-positivos com diarréia é baixa. Entretando, como a microsporidiose é mais frequentemente diagnosticada em pacientes com contagens de CD4 muito baixas, a indicação de pesquisa de microsporídios é justificada, especialmente para estes pacientes.
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- 2007
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46. Fetal radiation monitoring and dose minimization during intensity modulated radiation therapy for glioblastoma in pregnancy
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Horowitz, David P., Wang, Tony J. C., Wuu, Cheng-Shie, Feng, Wenzheng, Drassinower, Daphnie, Lasala, Anita, Pieniazek, Radoslaw, Cheng, Simon, Connolly, Eileen P., and Lassman, Andrew B.
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- 2014
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47. Mesenteric cysts in naevoid basal cell carcinoma syndrome: a mimic of metastatic disease
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Rajan, N., Brown, S., Ward, S., Hainsworth, P., Hodgkinson, P., Pieniazek, P., Husain, A., and Plummer, R.
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- 2016
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48. P5.18 TRANSRADIAL APPROACH FOR VERTEBRAL ARTERY STENTING: SINGLE-CENTER EXPERIENCE
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Damian Maciejewski, Lukasz Tekieli, Anna Kablak-Ziembicka, Karolina Dzierwa, Piotr Paluszek, Andrzej Brzychczy, Grzegorz Lewinski, Magdalena Wojcik-Pedziwiatr, Roman Machnik, and Piotr Pieniazek
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Specialties of internal medicine ,RC581-951 ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Objectives: Transradial vertebral artery (VA) angioplasty might be a viable alternative to the transfemoral approach in cases of peripheral artery disease or anatomical variations of the aortic arch. The purpose of our study was to evaluate the safety and efficacy of transradial stenting of symptomatic VA stenosis. Material and methods: Seventeen patients [67±8,4 years, 76% men, with >80% stenosis, 13 right-side, all symptomatic from posterior circulation (history of stroke, TIA or chronic ischemic symptoms)] with peripheral artery disease (PAD) or unsuccessful attempt via femoral access were scheduled for VA angioplasty by radial approach. Clinical and duplex ultrasound (DUS) follow-up were performed before discharge and 6, 12 and 24 months after VA stenting. Results: The technical success rate was 100%. In all cases VA angioplasty was performed with the use of single balloon-mounted stent (11 bare metal stents, 6 drug eluting stents). The mean NASCET VA stenosis was reduced from 87,5% to 5,9% (p
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- 2015
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49. P5.19 TRANSRADIAL APPROACH FOR CAROTID ARTERY STENTING: SINGLE-CENTER EXPERIENCE
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Damian Maciejewski, Lukasz Tekieli, Anna Kablak-Ziembicka, Piotr Paluszek, Mariusz Trystula, Karolina Dzierwa, Magdalena Wojcik-Pedziwiatr, Roman Machnik, Grzegorz Lewinski, and Piotr Pieniazek
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Specialties of internal medicine ,RC581-951 ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Objectives: The transfemoral approach is commonly used for carotid artery stenting (CAS) however in cases of severe peripheral vascular disease or unfavorable aortic arch anatomy, the transradial access remains a viable alternative. We report a series of patients with aorto-iliac disease or unsuccessful attempt via femoral access in whom transradial carotid artery stenting was performed. Material and methods: Sixteen patients (69±10,7 years, 75%men, with >70% stenosis, 10 left-side, 5 contralateral carotid occlusion, 9 with history of stroke or TIA) with peripheral artery disease (PAD) or unsuccessful attempt via femoral access were scheduled for carotid artery angioplasty by radial approach. Clinical and duplex ultrasound (DUS) follow-up were performed before discharge and 1, 12 and 24 months after carotid artery stenting. Results: The technical success rate was 87,5%. In two cases attempt via femoral and radial access were unsuccessful and the patients were treated by endarterectomy. In other cases CAS was performed with self-expanding bare metal stents. The mean NASCET carotid artery stenosis was reduced from 85% to 9,6% (p
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- 2015
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50. Archaeology across Frontiers and Borderlands : Fragmentation and Connectivity in the North Agean and the Central Balkans from the Bronze Age to the Iron Age
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Gimatzidis, Stefanos, Pieniążek, Magda, Mangaloğlu-Votruba, Sila, Gimatzidis, Stefanos, Pieniążek, Magda, and Mangaloğlu-Votruba, Sila
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- 2018
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